AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006753.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006753.01.T01 MTR_8g066600 80.303 66 11 1 43 108 17 80 3.62e-31 105
MsG0280006753.01.T01 MTR_2g014320 79.688 64 11 1 43 106 17 78 9.00e-30 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006753.01.T01 AT1G74340 72.222 54 15 0 55 108 27 80 2.01e-23 86.3
MsG0280006753.01.T01 AT1G74340 72.222 54 15 0 55 108 27 80 2.01e-23 86.3

Find 17 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCTCTTATGCATATTTAT+TGG 0.211849 2:-5849798 MsG0280006753.01.T01:CDS
ACATTATGCCTTCTTCTTCT+TGG 0.225894 2:+5849758 None:intergenic
TATTCACTTATTATACGTTT+TGG 0.247618 2:-5850011 MsG0280006753.01.T01:CDS
GCCTCTTATGCATATTTATT+GGG 0.258483 2:-5849797 MsG0280006753.01.T01:CDS
GGATTAATATGGCATATTCT+TGG 0.312708 2:+5849847 None:intergenic
GATTAATATGGCATATTCTT+GGG 0.324167 2:+5849848 None:intergenic
AGAGCAACACCAGCAGAAAC+AGG 0.394145 2:+5849825 None:intergenic
ACCCAATAAATATGCATAAG+AGG 0.411050 2:+5849796 None:intergenic
GAATATCGATAAGCTTATAA+AGG 0.417351 2:+5850029 None:intergenic
AGCAGAAACAGGGATTAATA+TGG 0.417601 2:+5849836 None:intergenic
ATATTAATCCCTGTTTCTGC+TGG 0.419614 2:-5849834 MsG0280006753.01.T01:CDS
GAATTAGCAGACAGAGCAGT+TGG 0.476638 2:-5850063 MsG0280006753.01.T01:CDS
AAATGATCATCATCTACAAA+GGG 0.512690 2:+5849891 None:intergenic
ATTAATATGGCATATTCTTG+GGG 0.547808 2:+5849849 None:intergenic
GAAATGATCATCATCTACAA+AGG 0.583886 2:+5849890 None:intergenic
GCTGAAATCCAAGAAGAAGA+AGG 0.589308 2:-5849766 MsG0280006753.01.T01:CDS
GAGCAACACCAGCAGAAACA+GGG 0.653237 2:+5849826 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAAGTATTTTAAATGTATTA+TGG - Chr2:5849925-5849944 MsG0280006753.01.T01:intron 15.0%
!!! CATAATACATTTAAAATACT+TGG + Chr2:5849927-5849946 None:intergenic 15.0%
!! AAAAACAATAGGAATCAAAA+TGG - Chr2:5849803-5849822 MsG0280006753.01.T01:CDS 20.0%
!!! ATTCACTTATTATACGTTTT+GGG - Chr2:5849878-5849897 MsG0280006753.01.T01:CDS 20.0%
!!! TATTCACTTATTATACGTTT+TGG - Chr2:5849877-5849896 MsG0280006753.01.T01:CDS 20.0%
! AAATGATCATCATCTACAAA+GGG + Chr2:5850000-5850019 None:intergenic 25.0%
! ATTAATATGGCATATTCTTG+GGG + Chr2:5850042-5850061 None:intergenic 25.0%
! CACAGTACAATAAACTTATT+AGG + Chr2:5849968-5849987 None:intergenic 25.0%
! CTTATTAGGAAGTTCAAATA+AGG + Chr2:5849954-5849973 None:intergenic 25.0%
! GAATATCGATAAGCTTATAA+AGG + Chr2:5849862-5849881 None:intergenic 25.0%
! GATTAATATGGCATATTCTT+GGG + Chr2:5850043-5850062 None:intergenic 25.0%
ACCCAATAAATATGCATAAG+AGG + Chr2:5850095-5850114 None:intergenic 30.0%
GAAATGATCATCATCTACAA+AGG + Chr2:5850001-5850020 None:intergenic 30.0%
GCCTCTTATGCATATTTATT+GGG - Chr2:5850091-5850110 MsG0280006753.01.T01:CDS 30.0%
GGATTAATATGGCATATTCT+TGG + Chr2:5850044-5850063 None:intergenic 30.0%
TGCCTCTTATGCATATTTAT+TGG - Chr2:5850090-5850109 MsG0280006753.01.T01:CDS 30.0%
!! TGTGTATTTTTGTTGTCAAC+AGG - Chr2:5849776-5849795 MsG0280006753.01.T01:CDS 30.0%
AGCAGAAACAGGGATTAATA+TGG + Chr2:5850055-5850074 None:intergenic 35.0%
CAACAGGAAGCAAAAACAAT+AGG - Chr2:5849792-5849811 MsG0280006753.01.T01:CDS 35.0%
!! ATATTAATCCCTGTTTCTGC+TGG - Chr2:5850054-5850073 MsG0280006753.01.T01:CDS 35.0%
GCTGAAATCCAAGAAGAAGA+AGG - Chr2:5850122-5850141 MsG0280006753.01.T01:CDS 40.0%
TACTTGGCATGTTACTAACC+AGG + Chr2:5849911-5849930 None:intergenic 40.0%
!!! TACGTTTTGGGTTATCATCC+TGG - Chr2:5849890-5849909 MsG0280006753.01.T01:CDS 40.0%
GAATTAGCAGACAGAGCAGT+TGG - Chr2:5849825-5849844 MsG0280006753.01.T01:CDS 45.0%
AGAGCAACACCAGCAGAAAC+AGG + Chr2:5850066-5850085 None:intergenic 50.0%
GAGCAACACCAGCAGAAACA+GGG + Chr2:5850065-5850084 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr2 gene 5849761 5850149 5849761 ID=MsG0280006753.01;Name=MsG0280006753.01
Chr2 mRNA 5849761 5850149 5849761 ID=MsG0280006753.01.T01;Parent=MsG0280006753.01;Name=MsG0280006753.01.T01;_AED=0.45;_eAED=0.52;_QI=0|0|0|1|0|0|2|0|108
Chr2 exon 5849999 5850149 5849999 ID=MsG0280006753.01.T01:exon:1383;Parent=MsG0280006753.01.T01
Chr2 exon 5849761 5849936 5849761 ID=MsG0280006753.01.T01:exon:1382;Parent=MsG0280006753.01.T01
Chr2 CDS 5849999 5850149 5849999 ID=MsG0280006753.01.T01:cds;Parent=MsG0280006753.01.T01
Chr2 CDS 5849761 5849936 5849761 ID=MsG0280006753.01.T01:cds;Parent=MsG0280006753.01.T01
Gene Sequence

>MsG0280006753.01.T01

ATGCTAACAGTCATCTGTGTATTTTTGTTGTCAACAGGAAGCAAAAACAATAGGAATCAAAATGGAATTAGCAGACAGAGCAGTTGGATTTCTATTGTCCTTTATAAGCTTATCGATATTCACTTATTATACGTTTTGGGTTATCATCCTGTTTATTGTACTGTGCTCTTTCAGCCCTTTGTAGATGATGATCATTTCGTACACAAGTATTTCTTGCCCCAAGAATATGCCATATTAATCCCTGTTTCTGCTGGTGTTGCTCTTATTTGCCTCTTATGCATATTTATTGGGTTCGTGATGCTGAAATCCAAGAAGAAGAAGGCATAA

Protein sequence

>MsG0280006753.01.T01

MLTVICVFLLSTGSKNNRNQNGISRQSSWISIVLYKLIDIHLLYVLGYHPVYCTVLFQPFVDDDHFVHKYFLPQEYAILIPVSAGVALICLLCIFIGFVMLKSKKKKA*