AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024930.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024930.01.T01 MTR_5g019550 56.923 65 23 1 4 68 147 206 6.95e-15 66.6
MsG0580024930.01.T01 MTR_5g019550 56.923 65 23 1 4 68 231 290 1.65e-14 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 24 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAGATTTGACTTTGAGAA+TGG 0.428051 5:+11761851 MsG0580024930.01.T01:CDS
GTTTCATAAGATTAAAAGTA+GGG 0.455775 5:-11761731 None:intergenic
GGAAGGAGGAGCTGCACCAT+GGG 0.477201 5:-11761709 None:intergenic
GTATCATATCCATCGAACTC+TGG 0.485353 5:-11761774 None:intergenic
TATCATATCCATCGAACTCT+GGG 0.486116 5:-11761773 None:intergenic
CGATGAGTCAGATGCTTCTA+CGG 0.496132 5:+11761646 None:intergenic
TCAGCTACTGAGAGAGGTAG+AGG 0.507163 5:+11761828 MsG0580024930.01.T01:CDS
CTTCTACGGTGGACTCTGGT+AGG 0.522842 5:+11761660 MsG0580024930.01.T01:CDS
CTACGGTGGACTCTGGTAGG+TGG 0.546694 5:+11761663 MsG0580024930.01.T01:CDS
ATAAGATTAAAAGTAGGGGA+AGG 0.548784 5:-11761726 None:intergenic
TACGGTGGACTCTGGTAGGT+GGG 0.563236 5:+11761664 MsG0580024930.01.T01:CDS
GAAGCCATGCAATGGAGTGC+AGG 0.564764 5:+11761804 MsG0580024930.01.T01:CDS
GGGAAGGAGGAGCTGCACCA+TGG 0.569302 5:-11761710 None:intergenic
GATCACTCCCCAGAGTTCGA+TGG 0.577805 5:+11761765 MsG0580024930.01.T01:CDS
TTCAGACAACTAACTGCCCA+TGG 0.578107 5:+11761693 MsG0580024930.01.T01:CDS
GATGCTTCTACGGTGGACTC+TGG 0.578522 5:+11761656 None:intergenic
TGTTTCATAAGATTAAAAGT+AGG 0.581701 5:-11761732 None:intergenic
AGATTAAAAGTAGGGGAAGG+AGG 0.584415 5:-11761723 None:intergenic
CGTATGATGAAGCCATGCAA+TGG 0.591870 5:+11761796 MsG0580024930.01.T01:CDS
TGAACCTGCACTCCATTGCA+TGG 0.609662 5:-11761808 None:intergenic
GCAGGTTCAGCTACTGAGAG+AGG 0.625141 5:+11761822 MsG0580024930.01.T01:CDS
TTTCATAAGATTAAAAGTAG+GGG 0.628709 5:-11761730 None:intergenic
TGAGTCAGATGCTTCTACGG+TGG 0.636061 5:+11761649 None:intergenic
ATCATATCCATCGAACTCTG+GGG 0.720256 5:-11761772 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTTCATAAGATTAAAAGTA+GGG - Chr5:11761734-11761753 None:intergenic 20.0%
!! TGTTTCATAAGATTAAAAGT+AGG - Chr5:11761735-11761754 None:intergenic 20.0%
!! TTTCATAAGATTAAAAGTAG+GGG - Chr5:11761733-11761752 None:intergenic 20.0%
ATAAGATTAAAAGTAGGGGA+AGG - Chr5:11761729-11761748 None:intergenic 30.0%
TATCATATCCATCGAACTCT+GGG - Chr5:11761776-11761795 None:intergenic 35.0%
! CTCAGATTTGACTTTGAGAA+TGG + Chr5:11761851-11761870 MsG0580024930.01.T01:CDS 35.0%
AGATTAAAAGTAGGGGAAGG+AGG - Chr5:11761726-11761745 None:intergenic 40.0%
ATCATATCCATCGAACTCTG+GGG - Chr5:11761775-11761794 None:intergenic 40.0%
GTATCATATCCATCGAACTC+TGG - Chr5:11761777-11761796 None:intergenic 40.0%
CGTATGATGAAGCCATGCAA+TGG + Chr5:11761796-11761815 MsG0580024930.01.T01:CDS 45.0%
TTCAGACAACTAACTGCCCA+TGG + Chr5:11761693-11761712 MsG0580024930.01.T01:CDS 45.0%
TCAGCTACTGAGAGAGGTAG+AGG + Chr5:11761828-11761847 MsG0580024930.01.T01:CDS 50.0%
TGAACCTGCACTCCATTGCA+TGG - Chr5:11761811-11761830 None:intergenic 50.0%
GAAGCCATGCAATGGAGTGC+AGG + Chr5:11761804-11761823 MsG0580024930.01.T01:CDS 55.0%
GATCACTCCCCAGAGTTCGA+TGG + Chr5:11761765-11761784 MsG0580024930.01.T01:CDS 55.0%
GCAGGTTCAGCTACTGAGAG+AGG + Chr5:11761822-11761841 MsG0580024930.01.T01:CDS 55.0%
! CTTCTACGGTGGACTCTGGT+AGG + Chr5:11761660-11761679 MsG0580024930.01.T01:CDS 55.0%
! TACGGTGGACTCTGGTAGGT+GGG + Chr5:11761664-11761683 MsG0580024930.01.T01:CDS 55.0%
GGAAGGAGGAGCTGCACCAT+GGG - Chr5:11761712-11761731 None:intergenic 60.0%
! CTACGGTGGACTCTGGTAGG+TGG + Chr5:11761663-11761682 MsG0580024930.01.T01:CDS 60.0%
GGGAAGGAGGAGCTGCACCA+TGG - Chr5:11761713-11761732 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 11761657 11761884 11761657 ID=MsG0580024930.01;Name=MsG0580024930.01
Chr5 mRNA 11761657 11761884 11761657 ID=MsG0580024930.01.T01;Parent=MsG0580024930.01;Name=MsG0580024930.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|75
Chr5 exon 11761657 11761884 11761657 ID=MsG0580024930.01.T01:exon:3159;Parent=MsG0580024930.01.T01
Chr5 CDS 11761657 11761884 11761657 ID=MsG0580024930.01.T01:cds;Parent=MsG0580024930.01.T01
Gene Sequence

>MsG0580024930.01.T01

ATGCTTCTACGGTGGACTCTGGTAGGTGGGTTAGTTTTCAGACAACTAACTGCCCATGGTGCAGCTCCTCCTTCCCCTACTTTTAATCTTATGAAACAGCACAATGCAGATCACTCCCCAGAGTTCGATGGATATGATACGTATGATGAAGCCATGCAATGGAGTGCAGGTTCAGCTACTGAGAGAGGTAGAGGCTCAGATTTGACTTTGAGAATGGCAGAGTCGTGA

Protein sequence

>MsG0580024930.01.T01

MLLRWTLVGGLVFRQLTAHGAAPPSPTFNLMKQHNADHSPEFDGYDTYDEAMQWSAGSATERGRGSDLTLRMAES*