Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024930.01.T01 | AES94893.1 | 56.923 | 65 | 23 | 1 | 4 | 68 | 147 | 206 | 5.74E-11 | 66.6 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048065.01 | MsG0580024930.01 | -0.818380 | 2.020055e-52 | 1.509818e-49 |
| MsG0080048727.01 | MsG0580024930.01 | -0.802727 | 4.889992e-49 | 2.413189e-46 |
| MsG0080048773.01 | MsG0580024930.01 | -0.838343 | 3.043319e-57 | 4.064188e-54 |
| MsG0080048802.01 | MsG0580024930.01 | -0.846868 | 1.657737e-59 | 2.892937e-56 |
| MsG0080048849.01 | MsG0580024930.01 | -0.853770 | 1.918158e-61 | 4.190513e-58 |
| MsG0080048865.01 | MsG0580024930.01 | -0.806672 | 7.342004e-50 | 4.008745e-47 |
| MsG0080048878.01 | MsG0580024930.01 | -0.830041 | 3.671056e-55 | 3.823526e-52 |
| MsG0080048938.01 | MsG0580024930.01 | -0.804002 | 2.662146e-49 | 1.357369e-46 |
| MsG0080049011.01 | MsG0580024930.01 | -0.849195 | 3.778458e-60 | 7.108247e-57 |
| MsG0080049027.01 | MsG0580024930.01 | 0.818985 | 1.473080e-52 | 1.119567e-49 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024930.01.T01 | MTR_5g019550 | 56.923 | 65 | 23 | 1 | 4 | 68 | 147 | 206 | 6.95e-15 | 66.6 |
| MsG0580024930.01.T01 | MTR_5g019550 | 56.923 | 65 | 23 | 1 | 4 | 68 | 231 | 290 | 1.65e-14 | 66.2 |
Find 24 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATTAAAAGTAGGGGAAGG+AGG | 0.584415 | 5:-11761723 | None:intergenic |
| ATAAGATTAAAAGTAGGGGA+AGG | 0.548784 | 5:-11761726 | None:intergenic |
| ATCATATCCATCGAACTCTG+GGG | 0.720256 | 5:-11761772 | None:intergenic |
| CGATGAGTCAGATGCTTCTA+CGG | 0.496132 | 5:+11761646 | None:intergenic |
| CGTATGATGAAGCCATGCAA+TGG | 0.591870 | 5:+11761796 | MsG0580024930.01.T01:CDS |
| CTACGGTGGACTCTGGTAGG+TGG | 0.546694 | 5:+11761663 | MsG0580024930.01.T01:CDS |
| CTCAGATTTGACTTTGAGAA+TGG | 0.428051 | 5:+11761851 | MsG0580024930.01.T01:CDS |
| CTTCTACGGTGGACTCTGGT+AGG | 0.522842 | 5:+11761660 | MsG0580024930.01.T01:CDS |
| GAAGCCATGCAATGGAGTGC+AGG | 0.564764 | 5:+11761804 | MsG0580024930.01.T01:CDS |
| GATCACTCCCCAGAGTTCGA+TGG | 0.577805 | 5:+11761765 | MsG0580024930.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATAAGATTAAAAGTAGGGGA+AGG | - | Chr5:11761729-11761748 | None:intergenic | 30.0% | |
| TATCATATCCATCGAACTCT+GGG | - | Chr5:11761776-11761795 | None:intergenic | 35.0% | |
| AGATTAAAAGTAGGGGAAGG+AGG | - | Chr5:11761726-11761745 | None:intergenic | 40.0% | |
| ATCATATCCATCGAACTCTG+GGG | - | Chr5:11761775-11761794 | None:intergenic | 40.0% | |
| GTATCATATCCATCGAACTC+TGG | - | Chr5:11761777-11761796 | None:intergenic | 40.0% | |
| CGTATGATGAAGCCATGCAA+TGG | + | Chr5:11761796-11761815 | MsG0580024930.01.T01:CDS | 45.0% | |
| TTCAGACAACTAACTGCCCA+TGG | + | Chr5:11761693-11761712 | MsG0580024930.01.T01:CDS | 45.0% | |
| TCAGCTACTGAGAGAGGTAG+AGG | + | Chr5:11761828-11761847 | MsG0580024930.01.T01:CDS | 50.0% | |
| TGAACCTGCACTCCATTGCA+TGG | - | Chr5:11761811-11761830 | None:intergenic | 50.0% | |
| GAAGCCATGCAATGGAGTGC+AGG | + | Chr5:11761804-11761823 | MsG0580024930.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 11761657 | 11761884 | 11761657 | ID=MsG0580024930.01;Name=MsG0580024930.01 |
| Chr5 | mRNA | 11761657 | 11761884 | 11761657 | ID=MsG0580024930.01.T01;Parent=MsG0580024930.01;Name=MsG0580024930.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|75 |
| Chr5 | exon | 11761657 | 11761884 | 11761657 | ID=MsG0580024930.01.T01:exon:3159;Parent=MsG0580024930.01.T01 |
| Chr5 | CDS | 11761657 | 11761884 | 11761657 | ID=MsG0580024930.01.T01:cds;Parent=MsG0580024930.01.T01 |
| Gene Sequence |
>MsG0580024930.01.T01 ATGCTTCTACGGTGGACTCTGGTAGGTGGGTTAGTTTTCAGACAACTAACTGCCCATGGTGCAGCTCCTCCTTCCCCTACTTTTAATCTTATGAAACAGCACAATGCAGATCACTCCCCAGAGTTCGATGGATATGATACGTATGATGAAGCCATGCAATGGAGTGCAGGTTCAGCTACTGAGAGAGGTAGAGGCTCAGATTTGACTTTGAGAATGGCAGAGTCGTGA |
| Protein sequence |
>MsG0580024930.01.T01 MLLRWTLVGGLVFRQLTAHGAAPPSPTFNLMKQHNADHSPEFDGYDTYDEAMQWSAGSATERGRGSDLTLRMAES* |
