AlfalfaGEDB | MsG0280007743.01

M. sativa cultivar ZhongmuNo.1 / MsG0280007743.01

KEGG: K19706
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007743.01.T01	KAG4946146.1	72.65	117	15	1	10	109	69	185	6.28E-52	175

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007743.01.T01	O48916	67.797	59	19	0	13	71	1	59	3.07E-21	87.8

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007743.01.T01	A0A3L6RDJ1	80.412	97	19	0	13	109	1	97	3.64e-45	152

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048809.01	MsG0280007743.01	0.823579	1.283780e-53	1.109796e-50
MsG0080049027.01	MsG0280007743.01	0.858763	6.588027e-63	1.700034e-59
MsG0180000240.01	MsG0280007743.01	0.807216	5.632565e-50	3.119204e-47
MsG0180001576.01	MsG0280007743.01	0.840155	1.031051e-57	1.455923e-54
MsG0180002750.01	MsG0280007743.01	0.804063	2.586244e-49	1.320742e-46
MsG0180003189.01	MsG0280007743.01	-0.817722	2.846265e-52	2.089018e-49
MsG0180003246.01	MsG0280007743.01	0.808954	2.402363e-50	1.392320e-47
MsG0180003357.01	MsG0280007743.01	-0.834556	2.798721e-56	3.333334e-53
MsG0180004599.01	MsG0280007743.01	-0.809645	1.707851e-50	1.008325e-47
MsG0180005220.01	MsG0280007743.01	-0.800565	1.357674e-48	6.342058e-46
MsG0180005240.01	MsG0280007743.01	-0.807139	5.847595e-50	3.231710e-47
MsG0180005354.01	MsG0280007743.01	-0.804372	2.229317e-49	1.147510e-46
MsG0180005446.01	MsG0280007743.01	-0.826199	3.092354e-54	2.880513e-51
MsG0180005515.01	MsG0280007743.01	0.838240	3.233585e-57	4.304436e-54
MsG0180005881.01	MsG0280007743.01	0.863098	3.171334e-64	9.501806e-61
MsG0280006306.01	MsG0280007743.01	0.810762	9.812403e-51	5.966284e-48
MsG0280006360.01	MsG0280007743.01	-0.800364	1.491803e-48	6.933410e-46
MsG0280006634.01	MsG0280007743.01	-0.815769	7.800720e-52	5.427671e-49
MsG0280006669.01	MsG0280007743.01	-0.855636	5.523369e-62	1.283582e-58
MsG0280007165.01	MsG0280007743.01	0.835758	1.392357e-56	1.719079e-53
MsG0280007743.01	MsG0280007864.01	0.832127	1.128619e-55	1.249851e-52
MsG0280007743.01	MsG0280008928.01	-0.812529	4.050614e-51	2.581693e-48
MsG0280007743.01	MsG0280008929.01	-0.811273	7.603196e-51	4.685794e-48
MsG0280007743.01	MsG0280011059.01	0.802598	5.200400e-49	2.557794e-46
MsG0280007743.01	MsG0280011073.01	-0.804335	2.270093e-49	1.167353e-46
MsG0280007743.01	MsG0280011287.01	-0.816218	6.191924e-52	4.361391e-49
MsG0280007743.01	MsG0280011376.01	-0.811770	5.927584e-51	3.701545e-48
MsG0280007743.01	MsG0380011895.01	-0.802528	5.374279e-49	2.638578e-46
MsG0280007743.01	MsG0380012184.01	-0.861503	9.799111e-64	2.779045e-60
MsG0280007743.01	MsG0380013853.01	-0.805534	1.274289e-49	6.758187e-47
MsG0280007743.01	MsG0380014225.01	0.814893	1.221892e-51	8.299514e-49
MsG0280007743.01	MsG0380014551.01	0.801695	7.976228e-49	3.833750e-46
MsG0280007743.01	MsG0380014866.01	-0.836604	8.488070e-57	1.074935e-53
MsG0280007743.01	MsG0380015166.01	-0.800297	1.539895e-48	7.144788e-46
MsG0280007743.01	MsG0380015215.01	0.806467	8.111561e-50	4.406043e-47
MsG0280007743.01	MsG0380015745.01	-0.804974	1.669229e-49	8.726787e-47
MsG0280007743.01	MsG0380015847.01	0.806552	7.781026e-50	4.235776e-47
MsG0280007743.01	MsG0380015849.01	0.825310	5.027381e-54	4.565677e-51
MsG0280007743.01	MsG0380016118.01	0.803627	3.185597e-49	1.608605e-46
MsG0280007743.01	MsG0380016182.01	-0.847981	8.195535e-60	1.482008e-56
MsG0280007743.01	MsG0380017263.01	0.853612	2.129750e-61	4.628718e-58
MsG0280007743.01	MsG0380017265.01	0.809468	1.864231e-50	1.095408e-47
MsG0280007743.01	MsG0380017512.01	-0.801526	8.637897e-49	4.134285e-46
MsG0280007743.01	MsG0380017625.01	-0.811351	7.312223e-51	4.515623e-48
MsG0280007743.01	MsG0480020327.01	0.862053	6.653404e-64	1.922347e-60
MsG0280007743.01	MsG0480021776.01	0.813764	2.169787e-51	1.429492e-48
MsG0280007743.01	MsG0480022239.01	-0.811646	6.309686e-51	3.926965e-48
MsG0280007743.01	MsG0480022243.01	-0.807974	3.889036e-50	2.196625e-47
MsG0280007743.01	MsG0480022246.01	-0.826347	2.852069e-54	2.667867e-51
MsG0280007743.01	MsG0480022248.01	-0.829760	4.297132e-55	4.440211e-52
MsG0280007743.01	MsG0480022392.01	0.800649	1.305090e-48	6.109592e-46
MsG0280007743.01	MsG0480022739.01	0.825873	3.697567e-54	3.412415e-51
MsG0280007743.01	MsG0480023974.01	0.803862	2.846399e-49	1.446102e-46
MsG0280007743.01	MsG0580024930.01	0.840010	1.124774e-57	1.581239e-54
MsG0280007743.01	MsG0580026488.01	0.804889	1.739476e-49	9.074695e-47
MsG0280007743.01	MsG0580028117.01	0.803415	3.524207e-49	1.769939e-46
MsG0280007743.01	MsG0580029514.01	0.839302	1.718622e-57	2.363454e-54
MsG0280007743.01	MsG0580029999.01	0.841117	5.771563e-58	8.395167e-55
MsG0280007743.01	MsG0680031377.01	0.819338	1.223686e-52	9.389490e-50
MsG0280007743.01	MsG0680031446.01	0.817311	3.522473e-52	2.556228e-49
MsG0280007743.01	MsG0680031815.01	-0.814722	1.332813e-51	9.012110e-49
MsG0280007743.01	MsG0680032516.01	-0.841958	3.464314e-58	5.173803e-55
MsG0280007743.01	MsG0680035707.01	-0.826798	2.225759e-54	2.108904e-51
MsG0280007743.01	MsG0780036978.01	-0.819131	1.364331e-52	1.040965e-49
MsG0280007743.01	MsG0780037082.01	-0.811435	7.010980e-51	4.339624e-48
MsG0280007743.01	MsG0780037330.01	-0.834488	2.912327e-56	3.461432e-53
MsG0280007743.01	MsG0780038907.01	-0.809396	1.931809e-50	1.132985e-47
MsG0280007743.01	MsG0780039867.01	0.810272	1.251989e-50	7.513681e-48
MsG0280007743.01	MsG0780039964.01	-0.807174	5.750674e-50	3.181125e-47
MsG0280007743.01	MsG0780040375.01	0.858811	6.376144e-63	1.647966e-59
MsG0280007743.01	MsG0780041015.01	0.852238	5.263068e-61	1.092706e-57
MsG0280007743.01	MsG0780041729.01	0.819628	1.051001e-52	8.130432e-50
MsG0280007743.01	MsG0880042743.01	-0.800057	1.722538e-48	7.943482e-46
MsG0280007743.01	MsG0880043885.01	-0.806905	6.554523e-50	3.600545e-47
MsG0280007743.01	MsG0880044389.01	0.821531	3.842385e-53	3.134017e-50
MsG0280007743.01	MsG0880046384.01	0.813716	2.223086e-51	1.462807e-48
MsG0280007743.01	MsG0880046877.01	0.801137	1.037747e-48	4.918608e-46
MsG0280007743.01	MsG0880046963.01	0.800575	1.351428e-48	6.314516e-46
MsG0280007743.01	MsG0880047152.01	-0.814032	1.893593e-51	1.256683e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007743.01.T01	MTR_2g031370	97.959	49	1	61	109	150	198	1.46e-29	106
MsG0280007743.01.T01	MTR_2g031370	79.661	59	12	13	71	1	59	1.01e-26	99.4
MsG0280007743.01.T01	MTR_2g031370	97.959	49	1	61	109	162	210	1.47e-29	107
MsG0280007743.01.T01	MTR_2g031370	80.328	61	12	11	71	11	71	4.27e-28	103
MsG0280007743.01.T01	MTR_4g094522	78.431	51	11	13	63	1	51	1.62e-23	87.8
MsG0280007743.01.T01	MTR_4g094525	75.000	48	12	13	60	1	48	7.23e-20	81.6
MsG0280007743.01.T01	MTR_4g094525	70.833	48	14	62	109	151	198	3.20e-18	77.0
MsG0280007743.01.T01	MTR_4g094525	70.833	48	14	62	109	120	167	1.42e-18	77.4

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007743.01.T01	AT2G34770	67.797	59	19	13	71	1	59	3.13e-22	87.8
MsG0280007743.01.T01	AT2G34770	69.388	49	15	61	109	150	198	2.10e-18	77.8
MsG0280007743.01.T01	AT4G20870	60.000	60	24	13	72	1	60	1.83e-20	83.2
MsG0280007743.01.T01	AT4G20870	53.846	52	24	58	109	147	198	1.59e-13	64.7
MsG0280007743.01.T01	AT4G20870	60.000	60	24	13	72	1	60	1.83e-20	83.2
MsG0280007743.01.T01	AT4G20870	53.846	52	24	58	109	147	198	1.59e-13	64.7

Find 37 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTATTTGGCGGTGGCTTAC+TGG	0.121516	2:+19876592	MsG0280007743.01.T01:CDS
TCAACTACTCCTGCTTTATT+TGG	0.215512	2:+19876578	MsG0280007743.01.T01:CDS
TTTCGGGGATGTTCAGTCTT+TGG	0.281707	2:-19876662	None:intergenic
GACCATGGTGCAAGTAATAA+TGG	0.337316	2:-19876637	None:intergenic
GTTCTATAGGTTGGACATCT+TGG	0.372108	2:+19874185	MsG0280007743.01.T01:intron
TTATTTGGCGGTGGCTTACT+GGG	0.435203	2:+19876593	MsG0280007743.01.T01:CDS
ACTACTCCTGCTTTATTTGG+CGG	0.450026	2:+19876581	MsG0280007743.01.T01:CDS
TCCCCGTTTCTTTCAAAATG+AGG	0.458220	2:+19874256	MsG0280007743.01.T01:CDS
AAAGTCCTGGGCAACCATCT+TGG	0.468070	2:-19873958	None:intergenic
TCAAATCAACTACAAAGTCC+TGG	0.474615	2:-19873971	None:intergenic
GGACCTTCCTTGCTAACAAT+GGG	0.475219	2:-19874236	None:intergenic
CAGCCCATTGTTAGCAAGGA+AGG	0.502882	2:+19874233	MsG0280007743.01.T01:CDS
CAGCACCAAGATGGTTGCCC+AGG	0.518070	2:+19873953	MsG0280007743.01.T01:CDS
AAGCCTGTACCTGGAAAACA+AGG	0.518297	2:-19874001	None:intergenic
GGGACCTTCCTTGCTAACAA+TGG	0.525396	2:-19874237	None:intergenic
AGCAGGAGTAGTTGAAGGGG+TGG	0.532131	2:-19876570	None:intergenic
ACCATGGTGCAAGTAATAAT+GGG	0.532977	2:-19876636	None:intergenic
ACTCCTGCTTTATTTGGCGG+TGG	0.533512	2:+19876584	MsG0280007743.01.T01:CDS
AATAAAGCAGGAGTAGTTGA+AGG	0.548583	2:-19876575	None:intergenic
CAAATCAACTACAAAGTCCT+GGG	0.550444	2:-19873970	None:intergenic
TTTGGCGTTCAGCACCAAGA+TGG	0.551359	2:+19873944	MsG0280007743.01.T01:CDS
CTTTCAAAATGAGGTTCTAG+AGG	0.555614	2:+19874265	MsG0280007743.01.T01:CDS
TTGGTGAAGCTTATGAAGAG+TGG	0.555652	2:+19874204	MsG0280007743.01.T01:CDS
AAGCCACCGCCAAATAAAGC+AGG	0.556526	2:-19876587	None:intergenic
TCATCAGCCCATTGTTAGCA+AGG	0.566774	2:+19874229	MsG0280007743.01.T01:CDS
CATCCACGGTTAAAGGAGGA+AGG	0.566845	2:-19876705	None:intergenic
GTTCAGTCTTTGGCTGACCA+TGG	0.566911	2:-19876652	None:intergenic
TGGTGAAGCTTATGAAGAGT+GGG	0.567564	2:+19874205	MsG0280007743.01.T01:CDS
TGGACTCCAGTTCTGGAACG+TGG	0.587302	2:+19876535	MsG0280007743.01.T01:intron
ATAAAGCAGGAGTAGTTGAA+GGG	0.615938	2:-19876574	None:intergenic
GCCTGTACCTGGAAAACAAG+GGG	0.619950	2:-19873999	None:intergenic
ACCCATTATTACTTGCACCA+TGG	0.623569	2:+19876635	MsG0280007743.01.T01:CDS
TGAACATCCCCGAAACCTGA+AGG	0.626671	2:+19876670	MsG0280007743.01.T01:CDS
ATCCACGGTTAAAGGAGGAA+GGG	0.633458	2:-19876704	None:intergenic
TTGCCATCCACGGTTAAAGG+AGG	0.641183	2:-19876709	None:intergenic
TAAAGCAGGAGTAGTTGAAG+GGG	0.675171	2:-19876573	None:intergenic
AGCCTGTACCTGGAAAACAA+GGG	0.717048	2:-19874000	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATTGAACAAATTTAAAAA+AGG	-	Chr2:19875899-19875918	None:intergenic	10.0%
!!	CTTCAAATAATAATAATTTA+AGG	+	Chr2:19876240-19876259	MsG0280007743.01.T01:intron	10.0%
!!!	TTAATTTTATGTGTAAATTT+AGG	+	Chr2:19874093-19874112	MsG0280007743.01.T01:intron	10.0%
!!!	TTTATAAAAAAACAAGTTTT+TGG	+	Chr2:19874399-19874418	MsG0280007743.01.T01:intron	10.0%
!!!	TTTTTTAAATTTGTTCAATT+TGG	+	Chr2:19875898-19875917	MsG0280007743.01.T01:intron	10.0%
!!	CATTGTTTAAAAACATAATT+TGG	+	Chr2:19876078-19876097	MsG0280007743.01.T01:intron	15.0%
!!	TAATGTGGTTAATTATTAAA+AGG	-	Chr2:19874781-19874800	None:intergenic	15.0%
!!!	AATTTGTATTTAATTTCATG+TGG	+	Chr2:19876204-19876223	MsG0280007743.01.T01:intron	15.0%
!!!	ACAAAATTTTAATGTTCTAT+AGG	+	Chr2:19874172-19874191	MsG0280007743.01.T01:intron	15.0%
!!!	ATTTGTATTTAATTTCATGT+GGG	+	Chr2:19876205-19876224	MsG0280007743.01.T01:intron	15.0%
!!!	ATTTTTCTTATTATGACAAA+TGG	+	Chr2:19876004-19876023	MsG0280007743.01.T01:intron	15.0%
!!!	CAAATTATGTTTTTAAACAA+TGG	-	Chr2:19876080-19876099	None:intergenic	15.0%
!!!	CAATAAAGTATTTGAAAATT+CGG	-	Chr2:19875619-19875638	None:intergenic	15.0%
!!!	TTTCTAGTTATATAACTTTT+GGG	+	Chr2:19874691-19874710	MsG0280007743.01.T01:intron	15.0%
!!!	TTTTCTAGTTATATAACTTT+TGG	+	Chr2:19874690-19874709	MsG0280007743.01.T01:intron	15.0%
!!	AAAAAAACTAATTACTCTGT+TGG	-	Chr2:19875710-19875729	None:intergenic	20.0%
!!	ACTTAAATAGTAAGATTAGA+TGG	+	Chr2:19876029-19876048	MsG0280007743.01.T01:intron	20.0%
!!	ATATTGACATATCTATATTG+AGG	-	Chr2:19876443-19876462	None:intergenic	20.0%
!!	CAATATAGATATGTCAATAT+TGG	+	Chr2:19876443-19876462	MsG0280007743.01.T01:intron	20.0%
!!	GTATATAACTCTATACAAAT+GGG	+	Chr2:19874994-19875013	MsG0280007743.01.T01:intron	20.0%
!!	GTTTAAAAACATAATTTGGA+AGG	+	Chr2:19876082-19876101	MsG0280007743.01.T01:intron	20.0%
!!	TGTATATAACTCTATACAAA+TGG	+	Chr2:19874993-19875012	MsG0280007743.01.T01:intron	20.0%
!!!	AACAAATAAATTGTTGTTTC+TGG	+	Chr2:19876299-19876318	MsG0280007743.01.T01:intron	20.0%
!!!	AATTTTAATGTTCTATAGGT+TGG	+	Chr2:19874176-19874195	MsG0280007743.01.T01:intron	20.0%
!!!	AGATAGCGAAATAATTTTTT+CGG	-	Chr2:19874491-19874510	None:intergenic	20.0%
!!!	ATTTGATGAAAATTTGTTGT+AGG	-	Chr2:19875958-19875977	None:intergenic	20.0%
!!!	ATTTTATAACCTTCTTTGAT+AGG	+	Chr2:19876265-19876284	MsG0280007743.01.T01:intron	20.0%
!!!	CATGTATTTTATTACGATAA+TGG	-	Chr2:19875222-19875241	None:intergenic	20.0%
!!!	CGAATTTTCAAATACTTTAT+TGG	+	Chr2:19875617-19875636	MsG0280007743.01.T01:intron	20.0%
!!!	CTGTTTTAAAAACAAATATC+CGG	-	Chr2:19876143-19876162	None:intergenic	20.0%
!!!	TAAGTTGATTTAAGAAATGT+TGG	+	Chr2:19875175-19875194	MsG0280007743.01.T01:intron	20.0%
!!!	TACATAACATTTGAAATCTA+AGG	-	Chr2:19874068-19874087	None:intergenic	20.0%
!!!	TTTTAATGTGGAAAAGAAAA+CGG	-	Chr2:19875095-19875114	None:intergenic	20.0%
!!!	TTTTTATCATGAATTCTTCT+TGG	+	Chr2:19875667-19875686	MsG0280007743.01.T01:intron	20.0%
!!!	TTTTTGAAAGAGGATTTTTA+CGG	-	Chr2:19874030-19874049	None:intergenic	20.0%
!	AAAATTCCATGAAGTAATGT+TGG	+	Chr2:19876498-19876517	MsG0280007743.01.T01:intron	25.0%
!	ATAAATCCAATATCGTTAAC+TGG	-	Chr2:19874384-19874403	None:intergenic	25.0%
!	ATATGGCAAAAAATAGATAC+CGG	-	Chr2:19875590-19875609	None:intergenic	25.0%
!	CACATGAACAATATAAGATT+AGG	-	Chr2:19874642-19874661	None:intergenic	25.0%
!	GAAAAAAAATTGAACCTGAT+TGG	-	Chr2:19875936-19875955	None:intergenic	25.0%
!	TATCGTAATAAAATACATGC+CGG	+	Chr2:19875223-19875242	MsG0280007743.01.T01:intron	25.0%
!	TGAAAATTCGGCATATAATA+TGG	-	Chr2:19875607-19875626	None:intergenic	25.0%
!	TGTCTCACTATATAAAAAGT+GGG	+	Chr2:19875265-19875284	MsG0280007743.01.T01:intron	25.0%
!	TTCATCAAATTACTCCTTTA+AGG	+	Chr2:19875968-19875987	MsG0280007743.01.T01:intron	25.0%
!!!	CATTTAACCAAAAAGCATTA+CGG	-	Chr2:19875455-19875474	None:intergenic	25.0%
!!!	GTTATATAACTTTTGGGTAA+AGG	+	Chr2:19874697-19874716	MsG0280007743.01.T01:intron	25.0%
!!!	TTTGGTTAAATGTTGCTTTA+GGG	+	Chr2:19875463-19875482	MsG0280007743.01.T01:intron	25.0%
!!!	TTTTGAAAGAGGATTTTTAC+GGG	-	Chr2:19874029-19874048	None:intergenic	25.0%
!!!	TTTTGGTTAAATGTTGCTTT+AGG	+	Chr2:19875462-19875481	MsG0280007743.01.T01:intron	25.0%
	AAAACACCAACATTACTTCA+TGG	-	Chr2:19876507-19876526	None:intergenic	30.0%
	AACAGAGTGAAGTATAAGAA+TGG	+	Chr2:19876158-19876177	MsG0280007743.01.T01:intron	30.0%
	AACAGCTATATTATTGTTCC+CGG	+	Chr2:19875568-19875587	MsG0280007743.01.T01:intron	30.0%
	AACATGCCAATCAAAATCAA+AGG	-	Chr2:19874726-19874745	None:intergenic	30.0%
	AAGATTAGGAGTGATTATTC+AGG	-	Chr2:19874628-19874647	None:intergenic	30.0%
	AATAATATAGCTGTTGCAGT+AGG	-	Chr2:19875563-19875582	None:intergenic	30.0%
	ACATTAAAACAAAGACCTAC+TGG	+	Chr2:19875106-19875125	MsG0280007743.01.T01:intron	30.0%
	ACCATACATTATCTTCTACA+TGG	+	Chr2:19875491-19875510	MsG0280007743.01.T01:intron	30.0%
	AGATTAGGAGTGATTATTCA+GGG	-	Chr2:19874627-19874646	None:intergenic	30.0%
	AGTTTCATCATGTGAGAAAA+AGG	-	Chr2:19874550-19874569	None:intergenic	30.0%
	ATCTTCATTTGCTTGTAATG+TGG	-	Chr2:19874796-19874815	None:intergenic	30.0%
	CAACTTATGATTTCATGCTA+TGG	-	Chr2:19875162-19875181	None:intergenic	30.0%
	GTGTCTCACTATATAAAAAG+TGG	+	Chr2:19875264-19875283	MsG0280007743.01.T01:intron	30.0%
	TATACAAACCAACATACAAC+TGG	-	Chr2:19874979-19874998	None:intergenic	30.0%
	TATGGCAAAAAATAGATACC+GGG	-	Chr2:19875589-19875608	None:intergenic	30.0%
	TTCATGCTATGGAAACAAAA+GGG	-	Chr2:19875151-19875170	None:intergenic	30.0%
	TTGAAATCTAAGGAATGACA+TGG	-	Chr2:19874058-19874077	None:intergenic	30.0%
	TTTCATGCTATGGAAACAAA+AGG	-	Chr2:19875152-19875171	None:intergenic	30.0%
!	AAGTTTACCGTAATGCTTTT+TGG	+	Chr2:19875445-19875464	MsG0280007743.01.T01:intron	30.0%
!	ACTTGAATTTTCCCTTTTGA+GGG	-	Chr2:19875735-19875754	None:intergenic	30.0%
!	CATGAATTCTTCTTGGTAAT+AGG	+	Chr2:19875674-19875693	MsG0280007743.01.T01:intron	30.0%
!	TCTTCTTGGTAATAGGAATT+TGG	+	Chr2:19875681-19875700	MsG0280007743.01.T01:intron	30.0%
!	TGGTAAAAATGTTTAGAGCA+AGG	+	Chr2:19874419-19874438	MsG0280007743.01.T01:intron	30.0%
!!	AACCTCATTTTGAAAGAAAC+GGG	-	Chr2:19874261-19874280	None:intergenic	30.0%
!!	AGGTTTCCTTTGATTTTGAT+TGG	+	Chr2:19874717-19874736	MsG0280007743.01.T01:intron	30.0%
!!	CATGGAGAAATTTTTGAAAG+AGG	-	Chr2:19874040-19874059	None:intergenic	30.0%
!!	GAACCTCATTTTGAAAGAAA+CGG	-	Chr2:19874262-19874281	None:intergenic	30.0%
!!	TGTTGGTGTTTTGTATTTCT+TGG	+	Chr2:19876515-19876534	MsG0280007743.01.T01:intron	30.0%
!!	TTGGTTAAATGTTGCTTTAG+GGG	+	Chr2:19875464-19875483	MsG0280007743.01.T01:intron	30.0%
!!!	AAGTACAGCTTTTAAAACCT+AGG	+	Chr2:19875780-19875799	MsG0280007743.01.T01:intron	30.0%
!!!	AGTAGGTCTTTGTTTTAATG+TGG	-	Chr2:19875107-19875126	None:intergenic	30.0%
!!!	AGTTTTTTTTCCCCTCAAAA+GGG	+	Chr2:19875721-19875740	MsG0280007743.01.T01:intron	30.0%
!!!	CACATGTCTACTTTTTATCA+GGG	+	Chr2:19874513-19874532	MsG0280007743.01.T01:intron	30.0%
!!!	TAATTTTTTCGGAGGAACTA+CGG	-	Chr2:19874480-19874499	None:intergenic	30.0%
!!!	TAGCGAAATAATTTTTTCGG+AGG	-	Chr2:19874488-19874507	None:intergenic	30.0%
!!!	TAGTTTTTTTTCCCCTCAAA+AGG	+	Chr2:19875720-19875739	MsG0280007743.01.T01:intron	30.0%
	AAAATTGAACCTGATTGGTG+AGG	-	Chr2:19875931-19875950	None:intergenic	35.0%
	AATAAAGCAGGAGTAGTTGA+AGG	-	Chr2:19876578-19876597	None:intergenic	35.0%
	ACCATGGTGCAAGTAATAAT+GGG	-	Chr2:19876639-19876658	None:intergenic	35.0%
	AGAGTGAAGTATAAGAATGG+TGG	+	Chr2:19876161-19876180	MsG0280007743.01.T01:intron	35.0%
	ATAAAGCAGGAGTAGTTGAA+GGG	-	Chr2:19876577-19876596	None:intergenic	35.0%
	ATTTGGAAGGAAAATCACCA+TGG	+	Chr2:19876095-19876114	MsG0280007743.01.T01:intron	35.0%
	CAAATCAACTACAAAGTCCT+GGG	-	Chr2:19873973-19873992	None:intergenic	35.0%
	CCACAGGTTTCTAATTAGAT+AGG	-	Chr2:19875865-19875884	None:intergenic	35.0%
	CCTATCTAATTAGAAACCTG+TGG	+	Chr2:19875862-19875881	MsG0280007743.01.T01:intron	35.0%
	CCTGATAAAAAGTAGACATG+TGG	-	Chr2:19874515-19874534	None:intergenic	35.0%
	GCCATGTAGAAGATAATGTA+TGG	-	Chr2:19875495-19875514	None:intergenic	35.0%
	GGAAAATTCAAGTCTCACAT+TGG	+	Chr2:19875742-19875761	MsG0280007743.01.T01:intron	35.0%
	GTGTTGTAGTAGTACAAAAC+TGG	-	Chr2:19876354-19876373	None:intergenic	35.0%
	TCAAATCAACTACAAAGTCC+TGG	-	Chr2:19873974-19873993	None:intergenic	35.0%
	TCACATGATGAAACTTCACT+GGG	+	Chr2:19874555-19874574	MsG0280007743.01.T01:intron	35.0%
	TCTAACTCAGCCTTACAAAA+CGG	-	Chr2:19875825-19875844	None:intergenic	35.0%
	TCTTAAGCTCCTATCAAAGA+AGG	-	Chr2:19876277-19876296	None:intergenic	35.0%
	TTCGATGTGAAACTCCTTAA+AGG	-	Chr2:19875985-19876004	None:intergenic	35.0%
	TTTGGAAGGAAAATCACCAT+GGG	+	Chr2:19876096-19876115	MsG0280007743.01.T01:intron	35.0%
!	CTTGAATTTTCCCTTTTGAG+GGG	-	Chr2:19875734-19875753	None:intergenic	35.0%
!	CTTTCAAAATGAGGTTCTAG+AGG	+	Chr2:19874265-19874284	MsG0280007743.01.T01:CDS	35.0%
!	GACTTGAATTTTCCCTTTTG+AGG	-	Chr2:19875736-19875755	None:intergenic	35.0%
!	TAGATGGATTCTTTGTAACG+TGG	+	Chr2:19876045-19876064	MsG0280007743.01.T01:intron	35.0%
!	TCAACTACTCCTGCTTTATT+TGG	+	Chr2:19876578-19876597	MsG0280007743.01.T01:CDS	35.0%
!	TGCTTTGAGTAACAGCTTAT+TGG	+	Chr2:19875406-19875425	MsG0280007743.01.T01:intron	35.0%
!!	ACCTCATTTTGAAAGAAACG+GGG	-	Chr2:19874260-19874279	None:intergenic	35.0%
!!	CCTTACAAAACGGACTAAAA+AGG	-	Chr2:19875815-19875834	None:intergenic	35.0%
!!	CCTTTTTAGTCCGTTTTGTA+AGG	+	Chr2:19875812-19875831	MsG0280007743.01.T01:intron	35.0%
!!!	CCACATGTCTACTTTTTATC+AGG	+	Chr2:19874512-19874531	MsG0280007743.01.T01:intron	35.0%
	AACAAGTCTCAAACCATCCA+TGG	-	Chr2:19875534-19875553	None:intergenic	40.0%
	AATATAGCTGTTGCAGTAGG+AGG	-	Chr2:19875560-19875579	None:intergenic	40.0%
	ACAACTGGAAGCCAAATAAC+TGG	-	Chr2:19874964-19874983	None:intergenic	40.0%
	ACAAGTCTCAAACCATCCAT+GGG	-	Chr2:19875533-19875552	None:intergenic	40.0%
	ACCCATTATTACTTGCACCA+TGG	+	Chr2:19876635-19876654	MsG0280007743.01.T01:CDS	40.0%
	AGATAGGACAGTCTTTGCTT+TGG	-	Chr2:19875849-19875868	None:intergenic	40.0%
	ATATAGCTGTTGCAGTAGGA+GGG	-	Chr2:19875559-19875578	None:intergenic	40.0%
	CTCACATGATGAAACTTCAC+TGG	+	Chr2:19874554-19874573	MsG0280007743.01.T01:intron	40.0%
	GACCATGGTGCAAGTAATAA+TGG	-	Chr2:19876640-19876659	None:intergenic	40.0%
	GATACAAGACTTAGCTGTTG+TGG	-	Chr2:19874308-19874327	None:intergenic	40.0%
	GCTGTTACTCAAAGCAGATA+TGG	-	Chr2:19875402-19875421	None:intergenic	40.0%
	GGTAGACCAGTTAACGATAT+TGG	+	Chr2:19874375-19874394	MsG0280007743.01.T01:intron	40.0%
	GTGGGACTTACATGATTTAG+TGG	+	Chr2:19875283-19875302	MsG0280007743.01.T01:intron	40.0%
	TAAAGCAGGAGTAGTTGAAG+GGG	-	Chr2:19876576-19876595	None:intergenic	40.0%
	TATTTCTTGGACTCCAGTTC+TGG	+	Chr2:19876528-19876547	MsG0280007743.01.T01:intron	40.0%
	TCCCCGTTTCTTTCAAAATG+AGG	+	Chr2:19874256-19874275	MsG0280007743.01.T01:CDS	40.0%
	TGGTGAAGCTTATGAAGAGT+GGG	+	Chr2:19874205-19874224	MsG0280007743.01.T01:CDS	40.0%
	TTGGTGAAGCTTATGAAGAG+TGG	+	Chr2:19874204-19874223	MsG0280007743.01.T01:CDS	40.0%
!	CAGCTAAGTCTTGTATCACT+TGG	+	Chr2:19874311-19874330	MsG0280007743.01.T01:intron	40.0%
!	GTTCTATAGGTTGGACATCT+TGG	+	Chr2:19874185-19874204	MsG0280007743.01.T01:intron	40.0%
!	TGGTTCTTGGCATTTTTGTG+TGG	+	Chr2:19875046-19875065	MsG0280007743.01.T01:intron	40.0%
!	TTTGGCTTCCAGTTGTATGT+TGG	+	Chr2:19874968-19874987	MsG0280007743.01.T01:intron	40.0%
!!	AAAACGGACTAAAAAGGCGA+GGG	-	Chr2:19875809-19875828	None:intergenic	40.0%
!!	ACTACTCCTGCTTTATTTGG+CGG	+	Chr2:19876581-19876600	MsG0280007743.01.T01:CDS	40.0%
!!	GTGCTTTTACCTTCAGGTTT+CGG	-	Chr2:19876682-19876701	None:intergenic	40.0%
!!	TGCTTTTACCTTCAGGTTTC+GGG	-	Chr2:19876681-19876700	None:intergenic	40.0%
!!!	TGAGACACACCCTGTTTTTA+GGG	-	Chr2:19875252-19875271	None:intergenic	40.0%
	AAAGGATGACTGACATCCAC+AGG	-	Chr2:19875881-19875900	None:intergenic	45.0%
	AAGCCTGTACCTGGAAAACA+AGG	-	Chr2:19874004-19874023	None:intergenic	45.0%
	AATCACCATGGGCACAACAT+TGG	+	Chr2:19876107-19876126	MsG0280007743.01.T01:intron	45.0%
	ACCACCATCAGAGCTATATG+AGG	-	Chr2:19875030-19875049	None:intergenic	45.0%
	AGCCTGTACCTGGAAAACAA+GGG	-	Chr2:19874003-19874022	None:intergenic	45.0%
	CAAGTCTCAAACCATCCATG+GGG	-	Chr2:19875532-19875551	None:intergenic	45.0%
	CAGAGAGAGATCTAACCAGT+AGG	-	Chr2:19875124-19875143	None:intergenic	45.0%
	GGACCTTCCTTGCTAACAAT+GGG	-	Chr2:19874239-19874258	None:intergenic	45.0%
	GGTGGGCAATACCAGTTATT+TGG	+	Chr2:19874950-19874969	MsG0280007743.01.T01:intron	45.0%
	TCATCAGCCCATTGTTAGCA+AGG	+	Chr2:19874229-19874248	MsG0280007743.01.T01:CDS	45.0%
	TTATTTGGCGGTGGCTTACT+GGG	+	Chr2:19876593-19876612	MsG0280007743.01.T01:CDS	45.0%
	TTTATTTGGCGGTGGCTTAC+TGG	+	Chr2:19876592-19876611	MsG0280007743.01.T01:CDS	45.0%
	TTTCGGGGATGTTCAGTCTT+TGG	-	Chr2:19876665-19876684	None:intergenic	45.0%
!	CAACATTGGCTGTTTGAAGC+CGG	+	Chr2:19876121-19876140	MsG0280007743.01.T01:intron	45.0%
!!	ATAGCTCTGATGGTGGTTCT+TGG	+	Chr2:19875033-19875052	MsG0280007743.01.T01:intron	45.0%
!!	ATGCCGGTTTCCCTAAAAAC+AGG	+	Chr2:19875239-19875258	MsG0280007743.01.T01:intron	45.0%
!!	CAAAACGGACTAAAAAGGCG+AGG	-	Chr2:19875810-19875829	None:intergenic	45.0%
!!	CTGTCCTCATATAGCTCTGA+TGG	+	Chr2:19875023-19875042	MsG0280007743.01.T01:intron	45.0%
!!	GCTTTTACCTTCAGGTTTCG+GGG	-	Chr2:19876680-19876699	None:intergenic	45.0%
!!	GTGAGACACACCCTGTTTTT+AGG	-	Chr2:19875253-19875272	None:intergenic	45.0%
!!	TCCTCATATAGCTCTGATGG+TGG	+	Chr2:19875026-19875045	MsG0280007743.01.T01:intron	45.0%
!!	TGCCGGTTTCCCTAAAAACA+GGG	+	Chr2:19875240-19875259	MsG0280007743.01.T01:intron	45.0%
!!!	AGGGAAGTGCTTTTACCTTC+AGG	-	Chr2:19876688-19876707	None:intergenic	45.0%
!!!	CACCCTGTTTTTAGGGAAAC+CGG	-	Chr2:19875245-19875264	None:intergenic	45.0%
!!!	GAATAAGCCCCTTGTTTTCC+AGG	+	Chr2:19873992-19874011	MsG0280007743.01.T01:CDS	45.0%
	AAAGTCCTGGGCAACCATCT+TGG	-	Chr2:19873961-19873980	None:intergenic	50.0%
	AACAGCCAATGTTGTGCCCA+TGG	-	Chr2:19876115-19876134	None:intergenic	50.0%
	AAGCCACCGCCAAATAAAGC+AGG	-	Chr2:19876590-19876609	None:intergenic	50.0%
	CAGCCCATTGTTAGCAAGGA+AGG	+	Chr2:19874233-19874252	MsG0280007743.01.T01:CDS	50.0%
	GAGCTATATGAGGACAGCTG+AGG	-	Chr2:19875020-19875039	None:intergenic	50.0%
	GCCTGTACCTGGAAAACAAG+GGG	-	Chr2:19874002-19874021	None:intergenic	50.0%
	GGGACCTTCCTTGCTAACAA+TGG	-	Chr2:19874240-19874259	None:intergenic	50.0%
	GGTTTCTCACCTCACCAATC+AGG	+	Chr2:19875919-19875938	MsG0280007743.01.T01:intron	50.0%
	GTTCAGTCTTTGGCTGACCA+TGG	-	Chr2:19876655-19876674	None:intergenic	50.0%
	TAAAAAGGCGAGGGTTGCCT+AGG	-	Chr2:19875800-19875819	None:intergenic	50.0%
	TATTGCCCACCAGACAGTGA+GGG	-	Chr2:19874941-19874960	None:intergenic	50.0%
	TGAACATCCCCGAAACCTGA+AGG	+	Chr2:19876670-19876689	MsG0280007743.01.T01:CDS	50.0%
	TTTGGCGTTCAGCACCAAGA+TGG	+	Chr2:19873944-19873963	MsG0280007743.01.T01:CDS	50.0%
!	AACCATCCATGGGGTGTTTG+TGG	-	Chr2:19875523-19875542	None:intergenic	50.0%
!	TTACGGGTTAAGCCTGTACC+TGG	-	Chr2:19874013-19874032	None:intergenic	50.0%
!!	ACTCCTGCTTTATTTGGCGG+TGG	+	Chr2:19876584-19876603	MsG0280007743.01.T01:CDS	50.0%
!!	GTATCACTTGGTGTACGCGT+GGG	+	Chr2:19874323-19874342	MsG0280007743.01.T01:intron	50.0%
!!	TATCACTTGGTGTACGCGTG+GGG	+	Chr2:19874324-19874343	MsG0280007743.01.T01:intron	50.0%
!!	TGTATCACTTGGTGTACGCG+TGG	+	Chr2:19874322-19874341	MsG0280007743.01.T01:intron	50.0%
	AGCAGGAGTAGTTGAAGGGG+TGG	-	Chr2:19876573-19876592	None:intergenic	55.0%
	AGTCCTTGACCCTCACTGTC+TGG	+	Chr2:19874929-19874948	MsG0280007743.01.T01:intron	55.0%
	CACCACAAACACCCCATGGA+TGG	+	Chr2:19875518-19875537	MsG0280007743.01.T01:intron	55.0%
	CTTGACCCTCACTGTCTGGT+GGG	+	Chr2:19874933-19874952	MsG0280007743.01.T01:intron	55.0%
	GCTTCACCACGTTCCAGAAC+TGG	-	Chr2:19876544-19876563	None:intergenic	55.0%
	GGAGATTGCTAGGCTAGGCT+AGG	+	Chr2:19874349-19874368	MsG0280007743.01.T01:intron	55.0%
	GTATTGCCCACCAGACAGTG+AGG	-	Chr2:19874942-19874961	None:intergenic	55.0%
	TTGCTAGGCTAGGCTAGGCT+AGG	+	Chr2:19874354-19874373	MsG0280007743.01.T01:intron	55.0%
!	CATCCATGGGGTGTTTGTGG+TGG	-	Chr2:19875520-19875539	None:intergenic	55.0%
!!	ATCACTTGGTGTACGCGTGG+GGG	+	Chr2:19874325-19874344	MsG0280007743.01.T01:intron	55.0%
!!	TGGACTCCAGTTCTGGAACG+TGG	+	Chr2:19876535-19876554	MsG0280007743.01.T01:intron	55.0%
!!!	GCCCCTTGTTTTCCAGGTAC+AGG	+	Chr2:19873998-19874017	MsG0280007743.01.T01:intron	55.0%
	CAGCACCAAGATGGTTGCCC+AGG	+	Chr2:19873953-19873972	MsG0280007743.01.T01:CDS	60.0%
	CCACCAGACAGTGAGGGTCA+AGG	-	Chr2:19874935-19874954	None:intergenic	60.0%
	CCTTGACCCTCACTGTCTGG+TGG	+	Chr2:19874932-19874951	MsG0280007743.01.T01:intron	60.0%
	CTGCCACCACAAACACCCCA+TGG	+	Chr2:19875514-19875533	MsG0280007743.01.T01:intron	60.0%
	GGGGTGGAGATTGCTAGGCT+AGG	+	Chr2:19874344-19874363	MsG0280007743.01.T01:intron	60.0%
	GCGTGGGGGTGGAGATTGCT+AGG	+	Chr2:19874339-19874358	MsG0280007743.01.T01:intron	65.0%
!!	ACTTGGTGTACGCGTGGGGG+TGG	+	Chr2:19874328-19874347	MsG0280007743.01.T01:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	19873927	19876718	19873927	ID=MsG0280007743.01;Name=MsG0280007743.01
Chr2	mRNA	19873927	19876718	19873927	ID=MsG0280007743.01.T01;Parent=MsG0280007743.01;Name=MsG0280007743.01.T01;_AED=0.46;_eAED=0.53;_QI=0\|0.5\|0.33\|1\|0\|0\|3\|0\|117
Chr2	exon	19873927	19874013	19873927	ID=MsG0280007743.01.T01:exon:10259;Parent=MsG0280007743.01.T01
Chr2	exon	19874194	19874286	19874194	ID=MsG0280007743.01.T01:exon:10260;Parent=MsG0280007743.01.T01
Chr2	exon	19876545	19876718	19876545	ID=MsG0280007743.01.T01:exon:10261;Parent=MsG0280007743.01.T01
Chr2	CDS	19873927	19874013	19873927	ID=MsG0280007743.01.T01:cds;Parent=MsG0280007743.01.T01
Chr2	CDS	19874194	19874286	19874194	ID=MsG0280007743.01.T01:cds;Parent=MsG0280007743.01.T01
Chr2	CDS	19876545	19876718	19876545	ID=MsG0280007743.01.T01:cds;Parent=MsG0280007743.01.T01

Gene Sequence	>MsG0280007743.01.T01 ATTAACACTTTTTTATTTTTGGCGTTCAGCACCAAGATGGTTGCCCAGGACTTTGTAGTTGATTTGAATAAGCCCCTTGTTTTCCAGGTTGGACATCTTGGTGAAGCTTATGAAGAGTGGGTTCATCAGCCCATTGTTAGCAAGGAAGGTCCCCGTTTCTTTCAAAATGAGGTTCTAGAGTTCTGGAACGTGGTGAAGCTCTTTTCCACCCCTTCAACTACTCCTGCTTTATTTGGCGGTGGCTTACTGGGTTATGTAATTTATGATTGCACCCATTATTACTTGCACCATGGTCAGCCAAAGACTGAACATCCCCGAAACCTGAAGGTAAAAGCACTTCCCTTCCTCCTTTAA
Protein sequence	>MsG0280007743.01.T01 INTFLFLAFSTKMVAQDFVVDLNKPLVFQVGHLGEAYEEWVHQPIVSKEGPRFFQNEVLEFWNVVKLFSTPSTTPALFGGGLLGYVIYDCTHYYLHHGQPKTEHPRNLKVKALPFLL*