Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000994.01.T01 | GAU27493.1 | 86.159 | 289 | 30 | 3 | 1 | 279 | 1 | 289 | 1.67E-171 | 485 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000994.01.T01 | Q700C7 | 44.109 | 331 | 114 | 7 | 19 | 278 | 14 | 344 | 2.14E-70 | 224 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000994.01.T01 | A0A2Z6MBN5 | 86.159 | 289 | 30 | 3 | 1 | 279 | 1 | 289 | 7.96e-172 | 485 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0180000994.01.T01 | TF | bHLH |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048357.01 | MsG0180000994.01 | 0.832232 | 1.062997e-55 | 1.180843e-52 |
| MsG0180000632.01 | MsG0180000994.01 | 0.813544 | 2.426327e-51 | 1.589098e-48 |
| MsG0180000994.01 | MsG0180001200.01 | 0.825868 | 3.706423e-54 | 3.420230e-51 |
| MsG0180000994.01 | MsG0380011501.01 | 0.801490 | 8.784567e-49 | 4.200755e-46 |
| MsG0180000994.01 | MsG0480018909.01 | 0.845612 | 3.642785e-59 | 6.104375e-56 |
| MsG0180000994.01 | MsG0480024022.01 | 0.804826 | 1.792573e-49 | 9.336173e-47 |
| MsG0180000994.01 | MsG0580029418.01 | 0.807188 | 5.709593e-50 | 3.159559e-47 |
| MsG0180000994.01 | MsG0680031707.01 | 0.822056 | 2.905352e-53 | 2.405265e-50 |
| MsG0180000994.01 | MsG0680035683.01 | 0.843627 | 1.247331e-58 | 1.963426e-55 |
| MsG0180000994.01 | MsG0780040966.01 | 0.820353 | 7.173344e-53 | 5.662586e-50 |
| MsG0180000994.01 | MsG0880041858.01 | 0.832593 | 8.653582e-56 | 9.716854e-53 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000994.01.T01 | MTR_1g028470 | 91.007 | 278 | 20 | 1 | 1 | 278 | 1 | 273 | 2.61e-175 | 485 |
| MsG0180000994.01.T01 | MTR_3g090850 | 57.929 | 309 | 81 | 13 | 15 | 278 | 5 | 309 | 6.89e-98 | 290 |
| MsG0180000994.01.T01 | MTR_2g022280 | 49.198 | 187 | 74 | 2 | 92 | 278 | 1 | 166 | 7.30e-54 | 173 |
| MsG0180000994.01.T01 | MTR_0194s0050 | 38.655 | 238 | 123 | 4 | 43 | 279 | 145 | 360 | 1.44e-45 | 158 |
| MsG0180000994.01.T01 | MTR_3g099620 | 36.123 | 227 | 127 | 3 | 54 | 278 | 79 | 289 | 2.21e-36 | 132 |
| MsG0180000994.01.T01 | MTR_7g113830 | 38.318 | 214 | 108 | 4 | 66 | 278 | 93 | 283 | 3.43e-35 | 129 |
| MsG0180000994.01.T01 | MTR_1g082480 | 39.487 | 195 | 89 | 4 | 91 | 278 | 135 | 307 | 9.73e-34 | 125 |
| MsG0180000994.01.T01 | MTR_1g082480 | 39.487 | 195 | 89 | 4 | 91 | 278 | 135 | 307 | 2.36e-33 | 124 |
| MsG0180000994.01.T01 | MTR_8g066770 | 36.410 | 195 | 90 | 5 | 91 | 278 | 113 | 280 | 1.11e-32 | 122 |
| MsG0180000994.01.T01 | MTR_5g064950 | 32.117 | 274 | 137 | 8 | 27 | 278 | 23 | 269 | 1.10e-28 | 111 |
| MsG0180000994.01.T01 | MTR_5g064950 | 32.117 | 274 | 137 | 8 | 27 | 278 | 23 | 269 | 2.99e-28 | 110 |
| MsG0180000994.01.T01 | MTR_8g024210 | 30.435 | 253 | 133 | 4 | 40 | 278 | 115 | 338 | 1.03e-24 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000994.01.T01 | AT5G53210 | 44.411 | 331 | 113 | 7 | 19 | 278 | 14 | 344 | 6.02e-72 | 225 |
| MsG0180000994.01.T01 | AT5G53210 | 44.109 | 331 | 114 | 7 | 19 | 278 | 14 | 344 | 2.19e-71 | 224 |
| MsG0180000994.01.T01 | AT3G06120 | 45.213 | 188 | 87 | 3 | 92 | 278 | 1 | 173 | 3.89e-45 | 151 |
| MsG0180000994.01.T01 | AT3G24140 | 40.758 | 211 | 113 | 2 | 69 | 279 | 177 | 375 | 2.72e-44 | 155 |
| MsG0180000994.01.T01 | AT3G24140 | 40.758 | 211 | 113 | 2 | 69 | 279 | 177 | 375 | 2.85e-44 | 155 |
| MsG0180000994.01.T01 | AT1G72210 | 35.500 | 200 | 84 | 5 | 91 | 278 | 124 | 290 | 1.03e-29 | 114 |
| MsG0180000994.01.T01 | AT1G72210 | 35.500 | 200 | 84 | 5 | 91 | 278 | 124 | 290 | 1.04e-29 | 114 |
| MsG0180000994.01.T01 | AT1G22490 | 35.638 | 188 | 97 | 3 | 91 | 278 | 114 | 277 | 4.35e-26 | 104 |
| MsG0180000994.01.T01 | AT1G22490 | 35.638 | 188 | 97 | 3 | 91 | 278 | 114 | 277 | 1.64e-25 | 103 |
| MsG0180000994.01.T01 | AT4G01460 | 35.450 | 189 | 101 | 2 | 90 | 278 | 113 | 280 | 2.98e-24 | 99.8 |
| MsG0180000994.01.T01 | AT4G01460 | 35.450 | 189 | 101 | 2 | 90 | 278 | 113 | 280 | 2.98e-24 | 99.8 |
| MsG0180000994.01.T01 | AT2G46810 | 32.804 | 189 | 95 | 2 | 91 | 278 | 120 | 277 | 7.68e-21 | 90.9 |
| MsG0180000994.01.T01 | AT2G46810 | 32.275 | 189 | 96 | 2 | 91 | 278 | 120 | 277 | 1.23e-20 | 89.7 |
| MsG0180000994.01.T01 | AT5G65320 | 34.000 | 200 | 108 | 4 | 79 | 278 | 93 | 268 | 1.96e-20 | 89.0 |
| MsG0180000994.01.T01 | AT5G65320 | 34.000 | 200 | 108 | 4 | 79 | 278 | 93 | 268 | 2.08e-20 | 89.0 |
| MsG0180000994.01.T01 | AT2G46810 | 32.275 | 189 | 96 | 2 | 91 | 278 | 180 | 337 | 3.32e-20 | 89.4 |
| MsG0180000994.01.T01 | AT2G46810 | 32.275 | 189 | 96 | 2 | 91 | 278 | 192 | 349 | 4.56e-20 | 89.0 |
| MsG0180000994.01.T01 | AT3G61950 | 31.638 | 177 | 85 | 2 | 91 | 265 | 126 | 268 | 4.87e-19 | 85.1 |
| MsG0180000994.01.T01 | AT3G61950 | 31.638 | 177 | 85 | 2 | 91 | 265 | 126 | 268 | 5.47e-19 | 85.5 |
| MsG0180000994.01.T01 | AT3G61950 | 31.638 | 177 | 85 | 2 | 91 | 265 | 177 | 319 | 7.72e-19 | 85.5 |
| MsG0180000994.01.T01 | AT3G61950 | 31.638 | 177 | 85 | 2 | 91 | 265 | 177 | 319 | 8.44e-19 | 85.5 |
| MsG0180000994.01.T01 | AT5G46690 | 56.164 | 73 | 30 | 1 | 91 | 161 | 87 | 159 | 1.28e-18 | 84.0 |
| MsG0180000994.01.T01 | AT5G46690 | 56.164 | 73 | 30 | 1 | 91 | 161 | 87 | 159 | 2.62e-18 | 83.6 |
Find 34 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTTGTTTCTTGGATTCT+AGG | 0.149526 | 1:+14230053 | None:intergenic |
| GCTATACTTGTTCACTATTA+AGG | 0.194492 | 1:-14229681 | MsG0180000994.01.T01:CDS |
| TATGGAGTTCTTGGACTAAT+TGG | 0.233920 | 1:+14229971 | None:intergenic |
| AACCGTTTCGTCACGAATTC+CGG | 0.237314 | 1:-14229789 | MsG0180000994.01.T01:CDS |
| CTCATTGATGTTATCATTGA+TGG | 0.240583 | 1:+14229883 | None:intergenic |
| AGCCATTGATATGGAGTTCT+TGG | 0.282669 | 1:+14229962 | None:intergenic |
| CTAATTGGTAAGATTATTCT+AGG | 0.295024 | 1:+14229986 | None:intergenic |
| CATTGTTGTAGCCATTGATA+TGG | 0.334687 | 1:+14229953 | None:intergenic |
| ATATTCAATGAAACTGAGTT+TGG | 0.348570 | 1:-14230728 | MsG0180000994.01.T01:CDS |
| AACAAGGTGTCAAGATCTAT+TGG | 0.363851 | 1:+14230761 | None:intergenic |
| ACCGTTTCGTCACGAATTCC+GGG | 0.393233 | 1:-14229788 | MsG0180000994.01.T01:CDS |
| TGTGACTTCTTCAATTATAG+AGG | 0.399402 | 1:-14230555 | MsG0180000994.01.T01:CDS |
| ATGGAAGAAGCTGAAGAAGT+TGG | 0.426375 | 1:+14229902 | None:intergenic |
| GGACTTAGTGGTAAAGGTCT+TGG | 0.429539 | 1:+14230007 | None:intergenic |
| TTCGTCACGAATTCCGGGGC+AGG | 0.440139 | 1:-14229783 | MsG0180000994.01.T01:CDS |
| AAGATTATTCTAGGACTTAG+TGG | 0.440624 | 1:+14229995 | None:intergenic |
| ATTCTAGGACTTAGTGGTAA+AGG | 0.492814 | 1:+14230001 | None:intergenic |
| CCCCGGAATTCGTGACGAAA+CGG | 0.495669 | 1:+14229787 | None:intergenic |
| TGTCGCGAATTCAACATCTT+CGG | 0.496233 | 1:-14229858 | MsG0180000994.01.T01:CDS |
| GTACAAAATGGAAACTCAAA+AGG | 0.509601 | 1:-14230786 | None:intergenic |
| GTCCAAGAACTCCATATCAA+TGG | 0.523072 | 1:-14229964 | MsG0180000994.01.T01:CDS |
| ATCTTCGGTTGCTGATGTTG+AGG | 0.535328 | 1:-14229843 | MsG0180000994.01.T01:CDS |
| TGAAACTGAGTTTGGTTCAG+AGG | 0.550414 | 1:-14230720 | MsG0180000994.01.T01:CDS |
| TTGGAGATGGCTCAGATGAA+AGG | 0.570619 | 1:+14229921 | None:intergenic |
| ACATCGTATGTCGCATATCA+CGG | 0.587471 | 1:-14230513 | MsG0180000994.01.T01:CDS |
| GATCAAGCATCAATAGTTGA+AGG | 0.590664 | 1:-14230118 | MsG0180000994.01.T01:CDS |
| AATAGTGAACAAGTATAGCA+TGG | 0.592880 | 1:+14229685 | None:intergenic |
| GAAGCTGAAGAAGTTGGAGA+TGG | 0.605113 | 1:+14229908 | None:intergenic |
| ATTATTCTAAGAGCCTGCCC+CGG | 0.625886 | 1:+14229770 | None:intergenic |
| CCGTTTCGTCACGAATTCCG+GGG | 0.670932 | 1:-14229787 | MsG0180000994.01.T01:CDS |
| ATAGAGGAACAAAACAACGA+TGG | 0.690625 | 1:-14230539 | MsG0180000994.01.T01:CDS |
| CAATGGCTACAACAATGTGG+TGG | 0.701552 | 1:-14229947 | MsG0180000994.01.T01:CDS |
| TATCAATGGCTACAACAATG+TGG | 0.711948 | 1:-14229950 | MsG0180000994.01.T01:CDS |
| GAATATATCAGATAACAACA+AGG | 0.741545 | 1:+14230745 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTTTAAATGAAACTAGTT+AGG | - | Chr1:14230098-14230117 | MsG0180000994.01.T01:CDS | 20.0% |
| !!! | ATTATTGAAGTTTTCCAAAA+TGG | + | Chr1:14229767-14229786 | None:intergenic | 20.0% |
| !!! | GTTTTAAATGAAACTAGTTA+GGG | - | Chr1:14230099-14230118 | MsG0180000994.01.T01:CDS | 20.0% |
| !!! | TTTTAAATGAAACTAGTTAG+GGG | - | Chr1:14230100-14230119 | MsG0180000994.01.T01:CDS | 20.0% |
| ! | ATATTCAATGAAACTGAGTT+TGG | - | Chr1:14229724-14229743 | MsG0180000994.01.T01:CDS | 25.0% |
| ! | ATTACTCTTCTATATGGTTT+AGG | - | Chr1:14230309-14230328 | MsG0180000994.01.T01:intron | 25.0% |
| ! | CAACTAATTACTCTTCTATA+TGG | - | Chr1:14230303-14230322 | MsG0180000994.01.T01:intron | 25.0% |
| ! | CTAATTGGTAAGATTATTCT+AGG | + | Chr1:14230469-14230488 | None:intergenic | 25.0% |
| ! | GAATATATCAGATAACAACA+AGG | + | Chr1:14229710-14229729 | None:intergenic | 25.0% |
| ! | TTACTCTTCTATATGGTTTA+GGG | - | Chr1:14230310-14230329 | MsG0180000994.01.T01:intron | 25.0% |
| !!! | AAGATGTTGTTTTTGAGTTT+TGG | + | Chr1:14229810-14229829 | None:intergenic | 25.0% |
| !!! | GTAAACTTTTCTTTGTTTCT+TGG | + | Chr1:14230410-14230429 | None:intergenic | 25.0% |
| AATAGTGAACAAGTATAGCA+TGG | + | Chr1:14230770-14230789 | None:intergenic | 30.0% | |
| CATAAACCAGACCAAATTTA+TGG | - | Chr1:14230134-14230153 | MsG0180000994.01.T01:intron | 30.0% | |
| CTCATTGATGTTATCATTGA+TGG | + | Chr1:14230572-14230591 | None:intergenic | 30.0% | |
| GCTATACTTGTTCACTATTA+AGG | - | Chr1:14230771-14230790 | MsG0180000994.01.T01:CDS | 30.0% | |
| TGTGACTTCTTCAATTATAG+AGG | - | Chr1:14229897-14229916 | MsG0180000994.01.T01:CDS | 30.0% | |
| ! | ACTCTTTTGACATAAAAGCA+AGG | + | Chr1:14230024-14230043 | None:intergenic | 30.0% |
| ! | AGAGGATCTATTTTCCATTT+TGG | - | Chr1:14229750-14229769 | MsG0180000994.01.T01:CDS | 30.0% |
| ! | TTCTTTGTTTCTTGGATTCT+AGG | + | Chr1:14230402-14230421 | None:intergenic | 30.0% |
| !! | AAGATTATTCTAGGACTTAG+TGG | + | Chr1:14230460-14230479 | None:intergenic | 30.0% |
| !! | GAGTTTTGGTCATTGATAAT+GGG | + | Chr1:14229796-14229815 | None:intergenic | 30.0% |
| !! | TGAGTTTTGGTCATTGATAA+TGG | + | Chr1:14229797-14229816 | None:intergenic | 30.0% |
| ATAGAGGAACAAAACAACGA+TGG | - | Chr1:14229913-14229932 | MsG0180000994.01.T01:CDS | 35.0% | |
| CATTGTTGTAGCCATTGATA+TGG | + | Chr1:14230502-14230521 | None:intergenic | 35.0% | |
| GATCAAGCATCAATAGTTGA+AGG | - | Chr1:14230334-14230353 | MsG0180000994.01.T01:intron | 35.0% | |
| TATCAATGGCTACAACAATG+TGG | - | Chr1:14230502-14230521 | MsG0180000994.01.T01:CDS | 35.0% | |
| TATGGAGTTCTTGGACTAAT+TGG | + | Chr1:14230484-14230503 | None:intergenic | 35.0% | |
| ! | AACAAGGTGTCAAGATCTAT+TGG | + | Chr1:14229694-14229713 | None:intergenic | 35.0% |
| ! | GATGTTGAGGTTAAGTTTTG+TGG | - | Chr1:14230622-14230641 | MsG0180000994.01.T01:CDS | 35.0% |
| ! | TGCTCATTTTCAGAAACTTG+AGG | + | Chr1:14229841-14229860 | None:intergenic | 35.0% |
| !! | ATTCTAGGACTTAGTGGTAA+AGG | + | Chr1:14230454-14230473 | None:intergenic | 35.0% |
| ACATCGTATGTCGCATATCA+CGG | - | Chr1:14229939-14229958 | MsG0180000994.01.T01:CDS | 40.0% | |
| ACCAGACCAAATTTATGGTC+TGG | - | Chr1:14230139-14230158 | MsG0180000994.01.T01:intron | 40.0% | |
| ACCAGACCATAAATTTGGTC+TGG | + | Chr1:14230143-14230162 | None:intergenic | 40.0% | |
| ACTGGACCAGACCATAAATT+TGG | + | Chr1:14230148-14230167 | None:intergenic | 40.0% | |
| AGCCATTGATATGGAGTTCT+TGG | + | Chr1:14230493-14230512 | None:intergenic | 40.0% | |
| ATGGAAGAAGCTGAAGAAGT+TGG | + | Chr1:14230553-14230572 | None:intergenic | 40.0% | |
| ATTGCATCAACCGTAGACTA+CGG | + | Chr1:14230196-14230215 | None:intergenic | 40.0% | |
| GTCCAAGAACTCCATATCAA+TGG | - | Chr1:14230488-14230507 | MsG0180000994.01.T01:CDS | 40.0% | |
| TAACTACGAACCGTAGTCTA+CGG | - | Chr1:14230183-14230202 | MsG0180000994.01.T01:intron | 40.0% | |
| TAGACTACGGTTCGTAGTTA+GGG | + | Chr1:14230183-14230202 | None:intergenic | 40.0% | |
| TGTCGCGAATTCAACATCTT+CGG | - | Chr1:14230594-14230613 | MsG0180000994.01.T01:CDS | 40.0% | |
| ! | TGAAACTGAGTTTGGTTCAG+AGG | - | Chr1:14229732-14229751 | MsG0180000994.01.T01:CDS | 40.0% |
| AACCGTTTCGTCACGAATTC+CGG | - | Chr1:14230663-14230682 | MsG0180000994.01.T01:CDS | 45.0% | |
| AGACTACGGTTCGTAGTTAG+GGG | + | Chr1:14230182-14230201 | None:intergenic | 45.0% | |
| ATCTTCGGTTGCTGATGTTG+AGG | - | Chr1:14230609-14230628 | MsG0180000994.01.T01:CDS | 45.0% | |
| CAATGGCTACAACAATGTGG+TGG | - | Chr1:14230505-14230524 | MsG0180000994.01.T01:CDS | 45.0% | |
| GAAGCTGAAGAAGTTGGAGA+TGG | + | Chr1:14230547-14230566 | None:intergenic | 45.0% | |
| GGACTTAGTGGTAAAGGTCT+TGG | + | Chr1:14230448-14230467 | None:intergenic | 45.0% | |
| GTAGACTACGGTTCGTAGTT+AGG | + | Chr1:14230184-14230203 | None:intergenic | 45.0% | |
| TTGGAGATGGCTCAGATGAA+AGG | + | Chr1:14230534-14230553 | None:intergenic | 45.0% | |
| ! | ATTATTCTAAGAGCCTGCCC+CGG | + | Chr1:14230685-14230704 | None:intergenic | 45.0% |
| ! | TTAGGGGTGTGCAAAAGAAC+TGG | + | Chr1:14230166-14230185 | None:intergenic | 45.0% |
| !!! | TAATTTTAATCTTAATAAAA+AGG | + | Chr1:14230240-14230259 | None:intergenic | 5.0% |
| ACCGTTTCGTCACGAATTCC+GGG | - | Chr1:14230664-14230683 | MsG0180000994.01.T01:CDS | 50.0% | |
| CCCCGGAATTCGTGACGAAA+CGG | + | Chr1:14230668-14230687 | None:intergenic | 55.0% | |
| CCGTTTCGTCACGAATTCCG+GGG | - | Chr1:14230665-14230684 | MsG0180000994.01.T01:CDS | 55.0% | |
| TTCGTCACGAATTCCGGGGC+AGG | - | Chr1:14230669-14230688 | MsG0180000994.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 14229673 | 14230801 | 14229673 | ID=MsG0180000994.01;Name=MsG0180000994.01 |
| Chr1 | mRNA | 14229673 | 14230801 | 14229673 | ID=MsG0180000994.01.T01;Parent=MsG0180000994.01;Name=MsG0180000994.01.T01;_AED=0.32;_eAED=0.32;_QI=0|0|0|1|1|1|2|0|279 |
| Chr1 | exon | 14230433 | 14230801 | 14230433 | ID=MsG0180000994.01.T01:exon:9761;Parent=MsG0180000994.01.T01 |
| Chr1 | exon | 14229673 | 14230143 | 14229673 | ID=MsG0180000994.01.T01:exon:9760;Parent=MsG0180000994.01.T01 |
| Chr1 | CDS | 14230433 | 14230801 | 14230433 | ID=MsG0180000994.01.T01:cds;Parent=MsG0180000994.01.T01 |
| Chr1 | CDS | 14229673 | 14230143 | 14229673 | ID=MsG0180000994.01.T01:cds;Parent=MsG0180000994.01.T01 |
| Gene Sequence |
| Protein sequence |
