Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsG0780040966.01.T01 | XP_003625674.1 | 100 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 3.26E-39 | 133 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsG0780040966.01.T01 | Q7XXN8 | 72.727 | 44 | 12 | 0 | 19 | 62 | 4 | 47 | 5.31E-19 | 75.1 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsG0780040966.01.T01 | G7L3V3 | 100.000 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 1.56e-39 | 133 |
Gene ID | Type | Classification |
---|
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsG0080048357.01 | MsG0780040966.01 | 0.901606 | 2.260243e-78 | 3.077150e-74 |
MsG0180000404.01 | MsG0780040966.01 | 0.803305 | 3.714930e-49 | 1.860399e-46 |
MsG0180000632.01 | MsG0780040966.01 | 0.848165 | 7.294582e-60 | 1.326725e-56 |
MsG0180000994.01 | MsG0780040966.01 | 0.820353 | 7.173344e-53 | 5.662586e-50 |
MsG0180001200.01 | MsG0780040966.01 | 0.865328 | 6.395637e-65 | 2.072594e-61 |
MsG0180001771.01 | MsG0780040966.01 | 0.846166 | 2.576219e-59 | 4.395041e-56 |
MsG0180003431.01 | MsG0780040966.01 | 0.817088 | 3.953296e-52 | 2.851505e-49 |
MsG0180003741.01 | MsG0780040966.01 | 0.861470 | 1.003434e-63 | 2.842437e-60 |
MsG0180005113.01 | MsG0780040966.01 | 0.808839 | 2.542458e-50 | 1.468990e-47 |
MsG0180005540.01 | MsG0780040966.01 | 0.834146 | 3.548404e-56 | 4.173635e-53 |
MsG0780040286.01 | MsG0780040966.01 | 0.812209 | 4.756830e-51 | 3.005963e-48 |
MsG0780040432.01 | MsG0780040966.01 | 0.819780 | 9.703455e-53 | 7.538972e-50 |
MsG0780040846.01 | MsG0780040966.01 | 0.867280 | 1.536898e-65 | 5.335505e-62 |
MsG0780040966.01 | MsG0780041205.01 | 0.804416 | 2.183534e-49 | 1.125158e-46 |
MsG0780040966.01 | MsG0780041259.01 | 0.843252 | 1.570822e-58 | 2.443261e-55 |
MsG0780040966.01 | MsG0780041676.01 | 0.824763 | 6.768063e-54 | 6.051782e-51 |
MsG0780040966.01 | MsG0780041698.01 | 0.838513 | 2.749619e-57 | 3.691321e-54 |
MsG0780040966.01 | MsG0880041858.01 | 0.863847 | 1.858071e-64 | 5.713754e-61 |
MsG0780040966.01 | MsG0880042442.01 | 0.846843 | 1.683951e-59 | 2.936535e-56 |
MsG0780040966.01 | MsG0880042958.01 | 0.813039 | 3.131694e-51 | 2.023055e-48 |
MsG0780040966.01 | MsG0880042959.01 | 0.815885 | 7.351780e-52 | 5.130815e-49 |
MsG0780040966.01 | MsG0880045585.01 | 0.833699 | 4.588892e-56 | 5.325743e-53 |
MsG0780040966.01 | MsG0880045586.01 | 0.835115 | 2.024730e-56 | 2.451891e-53 |
MsG0780040966.01 | MsG0880046636.01 | 0.807696 | 4.455593e-50 | 2.498317e-47 |
MsG0780040966.01 | MsG0880046942.01 | 0.804299 | 2.309563e-49 | 1.186559e-46 |
MsG0280010839.01 | MsG0780040966.01 | 0.804215 | 2.404057e-49 | 1.232420e-46 |
MsG0380011496.01 | MsG0780040966.01 | 0.848618 | 5.465375e-60 | 1.008765e-56 |
MsG0380011501.01 | MsG0780040966.01 | 0.852518 | 4.380266e-61 | 9.175978e-58 |
MsG0380011675.01 | MsG0780040966.01 | 0.823163 | 1.605751e-53 | 1.371911e-50 |
MsG0680031342.01 | MsG0780040966.01 | 0.828158 | 1.050494e-54 | 1.035700e-51 |
MsG0680031707.01 | MsG0780040966.01 | 0.877649 | 5.306171e-69 | 2.688830e-65 |
MsG0680031959.01 | MsG0780040966.01 | 0.844260 | 8.438516e-59 | 1.354707e-55 |
MsG0680033143.01 | MsG0780040966.01 | 0.838385 | 2.967060e-57 | 3.967704e-54 |
MsG0680034835.01 | MsG0780040966.01 | 0.813286 | 2.764380e-51 | 1.797759e-48 |
MsG0480018909.01 | MsG0780040966.01 | 0.889540 | 2.209844e-73 | 1.790343e-69 |
MsG0480019279.01 | MsG0780040966.01 | 0.819243 | 1.286361e-52 | 9.845566e-50 |
MsG0480020064.01 | MsG0780040966.01 | 0.804630 | 1.969605e-49 | 1.020549e-46 |
MsG0480020096.01 | MsG0780040966.01 | 0.824238 | 8.992968e-54 | 7.920129e-51 |
MsG0480024022.01 | MsG0780040966.01 | 0.852443 | 4.601125e-61 | 9.615680e-58 |
MsG0580024094.01 | MsG0780040966.01 | 0.800364 | 1.492369e-48 | 6.935953e-46 |
MsG0580025466.01 | MsG0780040966.01 | 0.805188 | 1.505834e-49 | 7.915636e-47 |
MsG0580026000.01 | MsG0780040966.01 | 0.811421 | 7.061760e-51 | 4.369329e-48 |
MsG0380016465.01 | MsG0780040966.01 | 0.887510 | 1.339641e-72 | 9.991054e-69 |
MsG0380016466.01 | MsG0780040966.01 | 0.816429 | 5.555402e-52 | 3.935299e-49 |
MsG0380017823.01 | MsG0780040966.01 | 0.833252 | 5.934644e-56 | 6.796551e-53 |
MsG0380017869.01 | MsG0780040966.01 | 0.833461 | 5.262358e-56 | 6.064322e-53 |
MsG0380017974.01 | MsG0780040966.01 | 0.815153 | 1.069724e-51 | 7.317447e-49 |
MsG0580027925.01 | MsG0780040966.01 | 0.837627 | 4.648853e-57 | 6.074515e-54 |
MsG0580029310.01 | MsG0780040966.01 | 0.843642 | 1.236234e-58 | 1.946831e-55 |
MsG0580029405.01 | MsG0780040966.01 | 0.845206 | 4.694013e-59 | 7.764205e-56 |
MsG0580029406.01 | MsG0780040966.01 | 0.823301 | 1.491259e-53 | 1.279034e-50 |
MsG0580029417.01 | MsG0780040966.01 | 0.877719 | 5.016659e-69 | 2.548964e-65 |
MsG0580029418.01 | MsG0780040966.01 | 0.889229 | 2.920001e-73 | 2.335573e-69 |
MsG0280007305.01 | MsG0780040966.01 | 0.860891 | 1.505783e-63 | 4.180337e-60 |
MsG0280007414.01 | MsG0780040966.01 | 0.843615 | 1.256350e-58 | 1.976858e-55 |
MsG0280008390.01 | MsG0780040966.01 | 0.826871 | 2.138852e-54 | 2.030836e-51 |
MsG0280008391.01 | MsG0780040966.01 | 0.833260 | 5.906476e-56 | 6.765910e-53 |
MsG0280008392.01 | MsG0780040966.01 | 0.833515 | 5.102625e-56 | 5.889992e-53 |
MsG0280008395.01 | MsG0780040966.01 | 0.851853 | 6.768603e-61 | 1.388138e-57 |
MsG0280008397.01 | MsG0780040966.01 | 0.809408 | 1.920451e-50 | 1.126704e-47 |
MsG0280010163.01 | MsG0780040966.01 | 0.800386 | 1.476981e-48 | 6.868206e-46 |
MsG0280010320.01 | MsG0780040966.01 | 0.832527 | 8.986779e-56 | 1.007110e-52 |
MsG0680035658.01 | MsG0780040966.01 | 0.836861 | 7.302033e-57 | 9.320004e-54 |
MsG0680035683.01 | MsG0780040966.01 | 0.819635 | 1.047051e-52 | 8.101717e-50 |
MsG0780036148.01 | MsG0780040966.01 | 0.829724 | 4.384752e-55 | 4.525755e-52 |
MsG0780036178.01 | MsG0780040966.01 | 0.817632 | 2.982239e-52 | 2.183336e-49 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsG0780040966.01.T01 | MTR_7g101850 | 100.000 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 3.95e-43 | 133 |
MsG0780040966.01.T01 | MTR_1g063830 | 57.143 | 63 | 26 | 1 | 1 | 62 | 3 | 65 | 2.41e-20 | 76.3 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsG0780040966.01.T01 | AT2G36985 | 72.727 | 44 | 12 | 0 | 19 | 62 | 4 | 47 | 5.42e-20 | 75.1 |
MsG0780040966.01.T01 | AT3G53232 | 70.588 | 34 | 10 | 0 | 24 | 57 | 11 | 44 | 1.21e-12 | 56.6 |
Find 10 sgRNAs with CRISPR-Local
Find 10 sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
GATGCTGATGAGTACTATTT+TGG | 0.208088 | 7:-84943197 | None:intergenic |
TGACCAAGTGACTACACTTT+TGG | 0.325622 | 7:-84943257 | None:intergenic |
TGTGATCCGTGCAAGTCTTT+TGG | 0.334940 | 7:+84943234 | MsG0780040966.01.T01:CDS |
AAAATCATTCATGGCAGTAA+AGG | 0.489511 | 7:+84943155 | None:intergenic |
TGGCCAAAAGTGTAGTCACT+TGG | 0.560139 | 7:+84943254 | MsG0780040966.01.T01:CDS |
CATCAGCATCAAGAACAAGA+AGG | 0.581729 | 7:+84943210 | MsG0780040966.01.T01:CDS |
ACAAAAGCATGGCGATGCAA+CGG | 0.595123 | 7:-84943311 | None:intergenic |
ATCAGCATCAAGAACAAGAA+GGG | 0.607865 | 7:+84943211 | MsG0780040966.01.T01:CDS |
CTCATGATGACACAAAAGCA+TGG | 0.644566 | 7:-84943322 | None:intergenic |
TAAAGGAAATTAGCAGCACA+AGG | 0.665511 | 7:+84943172 | MsG0780040966.01.T01:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|---|---|---|---|---|
ATCAGCATCAAGAACAAGAA+GGG | + | Chr7:84943211-84943230 | MsG0780040966.01.T01:CDS | 35.0% | |
TAAAGGAAATTAGCAGCACA+AGG | + | Chr7:84943172-84943191 | MsG0780040966.01.T01:CDS | 35.0% | |
! | GATGCTGATGAGTACTATTT+TGG | - | Chr7:84943200-84943219 | None:intergenic | 35.0% |
CATCAGCATCAAGAACAAGA+AGG | + | Chr7:84943210-84943229 | MsG0780040966.01.T01:CDS | 40.0% | |
CTCATGATGACACAAAAGCA+TGG | - | Chr7:84943325-84943344 | None:intergenic | 40.0% | |
! | TGACCAAGTGACTACACTTT+TGG | - | Chr7:84943260-84943279 | None:intergenic | 40.0% |
! | TTTTGGCCAAAAGACTTGCA+CGG | - | Chr7:84943243-84943262 | None:intergenic | 40.0% |
! | ACAAAAGCATGGCGATGCAA+CGG | - | Chr7:84943314-84943333 | None:intergenic | 45.0% |
! | TGGCCAAAAGTGTAGTCACT+TGG | + | Chr7:84943254-84943273 | MsG0780040966.01.T01:CDS | 45.0% |
! | TGTGATCCGTGCAAGTCTTT+TGG | + | Chr7:84943234-84943253 | MsG0780040966.01.T01:CDS | 45.0% |
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr7 | gene | 84943165 | 84943359 | 84943165 | ID=MsG0780040966.01;Name=MsG0780040966.01 |
Chr7 | mRNA | 84943165 | 84943359 | 84943165 | ID=MsG0780040966.01.T01;Parent=MsG0780040966.01;Name=MsG0780040966.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|64 |
Chr7 | exon | 84943165 | 84943359 | 84943165 | ID=MsG0780040966.01.T01:exon:40932;Parent=MsG0780040966.01.T01 |
Chr7 | CDS | 84943165 | 84943359 | 84943165 | ID=MsG0780040966.01.T01:cds;Parent=MsG0780040966.01.T01 |
Gene Sequence |
Protein sequence |