AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040966.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040966.01.T01 MTR_7g101850 100.000 64 0 0 1 64 1 64 3.95e-43 133
MsG0780040966.01.T01 MTR_1g063830 57.143 63 26 1 1 62 3 65 2.41e-20 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040966.01.T01 AT2G36985 72.727 44 12 0 19 62 4 47 5.42e-20 75.1
MsG0780040966.01.T01 AT3G53232 70.588 34 10 0 24 57 11 44 1.21e-12 56.6

Find 10 sgRNAs with CRISPR-Local

Find 10 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGCTGATGAGTACTATTT+TGG 0.208088 7:-84943197 None:intergenic
TGACCAAGTGACTACACTTT+TGG 0.325622 7:-84943257 None:intergenic
TGTGATCCGTGCAAGTCTTT+TGG 0.334940 7:+84943234 MsG0780040966.01.T01:CDS
AAAATCATTCATGGCAGTAA+AGG 0.489511 7:+84943155 None:intergenic
TGGCCAAAAGTGTAGTCACT+TGG 0.560139 7:+84943254 MsG0780040966.01.T01:CDS
CATCAGCATCAAGAACAAGA+AGG 0.581729 7:+84943210 MsG0780040966.01.T01:CDS
ACAAAAGCATGGCGATGCAA+CGG 0.595123 7:-84943311 None:intergenic
ATCAGCATCAAGAACAAGAA+GGG 0.607865 7:+84943211 MsG0780040966.01.T01:CDS
CTCATGATGACACAAAAGCA+TGG 0.644566 7:-84943322 None:intergenic
TAAAGGAAATTAGCAGCACA+AGG 0.665511 7:+84943172 MsG0780040966.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATCAGCATCAAGAACAAGAA+GGG + Chr7:84943211-84943230 MsG0780040966.01.T01:CDS 35.0%
TAAAGGAAATTAGCAGCACA+AGG + Chr7:84943172-84943191 MsG0780040966.01.T01:CDS 35.0%
! GATGCTGATGAGTACTATTT+TGG - Chr7:84943200-84943219 None:intergenic 35.0%
CATCAGCATCAAGAACAAGA+AGG + Chr7:84943210-84943229 MsG0780040966.01.T01:CDS 40.0%
CTCATGATGACACAAAAGCA+TGG - Chr7:84943325-84943344 None:intergenic 40.0%
! TGACCAAGTGACTACACTTT+TGG - Chr7:84943260-84943279 None:intergenic 40.0%
! TTTTGGCCAAAAGACTTGCA+CGG - Chr7:84943243-84943262 None:intergenic 40.0%
! ACAAAAGCATGGCGATGCAA+CGG - Chr7:84943314-84943333 None:intergenic 45.0%
! TGGCCAAAAGTGTAGTCACT+TGG + Chr7:84943254-84943273 MsG0780040966.01.T01:CDS 45.0%
! TGTGATCCGTGCAAGTCTTT+TGG + Chr7:84943234-84943253 MsG0780040966.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr7 gene 84943165 84943359 84943165 ID=MsG0780040966.01;Name=MsG0780040966.01
Chr7 mRNA 84943165 84943359 84943165 ID=MsG0780040966.01.T01;Parent=MsG0780040966.01;Name=MsG0780040966.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|64
Chr7 exon 84943165 84943359 84943165 ID=MsG0780040966.01.T01:exon:40932;Parent=MsG0780040966.01.T01
Chr7 CDS 84943165 84943359 84943165 ID=MsG0780040966.01.T01:cds;Parent=MsG0780040966.01.T01
Gene Sequence

>MsG0780040966.01.T01

ATGGCAGTAAAGGAAATTAGCAGCACAAGGAACCAAAATAGTACTCATCAGCATCAAGAACAAGAAGGGTGTGATCCGTGCAAGTCTTTTGGCCAAAAGTGTAGTCACTTGGTCAAGAAACAGCGTGCAAAATTCTACATTCTTCGCCGTTGCATCGCCATGCTTTTGTGTCATCATGAGCGTTCTGAGCATTGA

Protein sequence

>MsG0780040966.01.T01

MAVKEISSTRNQNSTHQHQEQEGCDPCKSFGQKCSHLVKKQRAKFYILRRCIAMLLCHHERSEH*