Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001854.01.T01 | XP_013462384.1 | 92.683 | 123 | 4 | 1 | 10 | 132 | 193 | 310 | 2.64E-72 | 233 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001854.01.T01 | Q9C518 | 52.294 | 109 | 38 | 5 | 10 | 110 | 123 | 225 | 1.63E-14 | 72 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001854.01.T01 | A0A072V348 | 92.683 | 123 | 4 | 1 | 10 | 132 | 193 | 310 | 1.26e-72 | 233 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000402.01 | MsG0180001854.01 | 0.802405 | 5.699383e-49 | 2.789583e-46 |
| MsG0180001854.01 | MsG0180005507.01 | 0.814789 | 1.288360e-51 | 8.726909e-49 |
| MsG0180001854.01 | MsG0280006692.01 | 0.818072 | 2.372065e-52 | 1.757687e-49 |
| MsG0180001854.01 | MsG0280007764.01 | 0.804206 | 2.413958e-49 | 1.237221e-46 |
| MsG0180001854.01 | MsG0280011334.01 | 0.801879 | 7.310851e-49 | 3.530763e-46 |
| MsG0180001854.01 | MsG0380017055.01 | 0.809058 | 2.282731e-50 | 1.326708e-47 |
| MsG0180001854.01 | MsG0480020242.01 | 0.806656 | 7.397902e-50 | 4.037589e-47 |
| MsG0180001854.01 | MsG0480022598.01 | 0.808339 | 3.250872e-50 | 1.854027e-47 |
| MsG0180001854.01 | MsG0480023451.01 | 0.869235 | 3.603159e-66 | 1.342280e-62 |
| MsG0180001854.01 | MsG0480023670.01 | 0.821898 | 3.161349e-53 | 2.605607e-50 |
| MsG0180001854.01 | MsG0480023718.01 | 0.824137 | 9.502063e-54 | 8.344514e-51 |
| MsG0180001854.01 | MsG0580024157.01 | 0.822797 | 1.954836e-53 | 1.652949e-50 |
| MsG0180001854.01 | MsG0580024477.01 | 0.800076 | 1.707628e-48 | 7.878439e-46 |
| MsG0180001854.01 | MsG0580025206.01 | 0.813048 | 3.117205e-51 | 2.014160e-48 |
| MsG0180001854.01 | MsG0580025532.01 | 0.812980 | 3.227026e-51 | 2.081274e-48 |
| MsG0180001854.01 | MsG0580025555.01 | 0.823005 | 1.748086e-53 | 1.486948e-50 |
| MsG0180001854.01 | MsG0680032831.01 | 0.806139 | 9.507643e-50 | 5.121466e-47 |
| MsG0180001854.01 | MsG0780036630.01 | 0.801315 | 9.543799e-49 | 4.543660e-46 |
| MsG0180001854.01 | MsG0780039655.01 | 0.824050 | 9.956915e-54 | 8.722042e-51 |
| MsG0180001854.01 | MsG0780041764.01 | 0.802066 | 6.692863e-49 | 3.247529e-46 |
| MsG0180001854.01 | MsG0880046560.01 | 0.817187 | 3.756384e-52 | 2.716753e-49 |
| MsG0180001854.01 | MsG0880047337.01 | 0.811435 | 7.012354e-51 | 4.340435e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001854.01.T01 | MTR_2g006150 | 92.683 | 123 | 4 | 1 | 10 | 132 | 193 | 310 | 3.20e-76 | 233 |
| MsG0180001854.01.T01 | MTR_2g006150 | 92.683 | 123 | 4 | 1 | 10 | 132 | 193 | 310 | 1.56e-75 | 232 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001854.01.T01 | AT1G58100 | 52.294 | 109 | 38 | 5 | 10 | 110 | 99 | 201 | 1.64e-15 | 72.0 |
| MsG0180001854.01.T01 | AT1G58100 | 52.294 | 109 | 38 | 5 | 10 | 110 | 123 | 225 | 1.66e-15 | 72.0 |
Find 19 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACCAACACGCGCCTTTGTT+AGG | 0.309870 | 1:-28282118 | MsG0180001854.01.T01:CDS |
| GTTGTTGTTGTTGCTGTTGT+TGG | 0.373330 | 1:+28282074 | None:intergenic |
| CTTATGAATGCTGATCAAAT+TGG | 0.428980 | 1:-28282049 | MsG0180001854.01.T01:CDS |
| ACCAACACGCGCCTTTGTTA+GGG | 0.449441 | 1:-28282117 | MsG0180001854.01.T01:CDS |
| AGAGTGTAGAGCCGCTGCTA+CGG | 0.485412 | 1:+28282221 | None:intergenic |
| ACGAGAATTACATGAGAAAA+CGG | 0.506668 | 1:-28282012 | MsG0180001854.01.T01:CDS |
| GTTGTTGTTGCTGTTGTTGG+TGG | 0.514677 | 1:+28282077 | None:intergenic |
| CAAAATGAAAGTGGCGGTGA+TGG | 0.520198 | 1:-28281953 | MsG0180001854.01.T01:CDS |
| GCTTCTTCAACAAGCGAAGC+CGG | 0.522046 | 1:-28282271 | MsG0180001854.01.T01:CDS |
| AACAACAATCAAAATGAAAG+TGG | 0.537872 | 1:-28281962 | MsG0180001854.01.T01:CDS |
| TGTTGTTGGTGGTGTTGATG+AGG | 0.542903 | 1:+28282088 | None:intergenic |
| ACCCTAACAAAGGCGCGTGT+TGG | 0.563265 | 1:+28282116 | None:intergenic |
| GAAAGTGGCGGTGATGGTGA+TGG | 0.632003 | 1:-28281947 | MsG0180001854.01.T01:CDS |
| GTTGAGCGTTGAGAAATCGC+CGG | 0.633923 | 1:+28282252 | None:intergenic |
| AACGTCTCACTCCGTAGCAG+CGG | 0.639266 | 1:-28282232 | MsG0180001854.01.T01:CDS |
| AACAATCAAAATGAAAGTGG+CGG | 0.649002 | 1:-28281959 | MsG0180001854.01.T01:CDS |
| AGAGGAGCTGATTTCGACGA+CGG | 0.652609 | 1:+28282193 | None:intergenic |
| TGAGGATGAAACCCTAACAA+AGG | 0.670241 | 1:+28282106 | None:intergenic |
| AGCGCGCTATGAAAACTGAG+AGG | 0.721312 | 1:+28282175 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGATTTGTTTTGAAACTTT+TGG | + | Chr1:28282256-28282275 | None:intergenic | 20.0% |
| !!! | TGATTTGTTTTGAAACTTTT+GGG | + | Chr1:28282255-28282274 | None:intergenic | 20.0% |
| ! | AACAACAATCAAAATGAAAG+TGG | - | Chr1:28282204-28282223 | MsG0180001854.01.T01:CDS | 25.0% |
| AACAATCAAAATGAAAGTGG+CGG | - | Chr1:28282207-28282226 | MsG0180001854.01.T01:CDS | 30.0% | |
| ACGAGAATTACATGAGAAAA+CGG | - | Chr1:28282154-28282173 | MsG0180001854.01.T01:CDS | 30.0% | |
| CTTATGAATGCTGATCAAAT+TGG | - | Chr1:28282117-28282136 | MsG0180001854.01.T01:CDS | 30.0% | |
| TGAGGATGAAACCCTAACAA+AGG | + | Chr1:28282063-28282082 | None:intergenic | 40.0% | |
| ! | GTTGTTGTTGTTGCTGTTGT+TGG | + | Chr1:28282095-28282114 | None:intergenic | 40.0% |
| CAAAATGAAAGTGGCGGTGA+TGG | - | Chr1:28282213-28282232 | MsG0180001854.01.T01:CDS | 45.0% | |
| ! | GTTGTTGTTGCTGTTGTTGG+TGG | + | Chr1:28282092-28282111 | None:intergenic | 45.0% |
| !! | TGTTGTTGGTGGTGTTGATG+AGG | + | Chr1:28282081-28282100 | None:intergenic | 45.0% |
| ACCAACACGCGCCTTTGTTA+GGG | - | Chr1:28282049-28282068 | MsG0180001854.01.T01:CDS | 50.0% | |
| AGCGCGCTATGAAAACTGAG+AGG | + | Chr1:28281994-28282013 | None:intergenic | 50.0% | |
| GCTTCTTCAACAAGCGAAGC+CGG | - | Chr1:28281895-28281914 | MsG0180001854.01.T01:CDS | 50.0% | |
| TACCAACACGCGCCTTTGTT+AGG | - | Chr1:28282048-28282067 | MsG0180001854.01.T01:CDS | 50.0% | |
| ! | AGAGGAGCTGATTTCGACGA+CGG | + | Chr1:28281976-28281995 | None:intergenic | 50.0% |
| ! | TTTTCATAGCGCGCTTGCAC+TGG | - | Chr1:28281999-28282018 | MsG0180001854.01.T01:CDS | 50.0% |
| !! | GTTGAGCGTTGAGAAATCGC+CGG | + | Chr1:28281917-28281936 | None:intergenic | 50.0% |
| AACGTCTCACTCCGTAGCAG+CGG | - | Chr1:28281934-28281953 | MsG0180001854.01.T01:CDS | 55.0% | |
| AGAGTGTAGAGCCGCTGCTA+CGG | + | Chr1:28281948-28281967 | None:intergenic | 55.0% | |
| ! | ACCCTAACAAAGGCGCGTGT+TGG | + | Chr1:28282053-28282072 | None:intergenic | 55.0% |
| ! | GAAAGTGGCGGTGATGGTGA+TGG | - | Chr1:28282219-28282238 | MsG0180001854.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 28281892 | 28282296 | 28281892 | ID=MsG0180001854.01;Name=MsG0180001854.01 |
| Chr1 | mRNA | 28281892 | 28282296 | 28281892 | ID=MsG0180001854.01.T01;Parent=MsG0180001854.01;Name=MsG0180001854.01.T01;_AED=0.41;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|134 |
| Chr1 | exon | 28281892 | 28282296 | 28281892 | ID=MsG0180001854.01.T01:exon:18741;Parent=MsG0180001854.01.T01 |
| Chr1 | CDS | 28281892 | 28282296 | 28281892 | ID=MsG0180001854.01.T01:cds;Parent=MsG0180001854.01.T01 |
| Gene Sequence |
| Protein sequence |
