Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023451.01.T01 | XP_003630199.1 | 85.774 | 239 | 28 | 3 | 1 | 236 | 1 | 236 | 6.89E-134 | 397 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023451.01.T01 | Q9FLF4 | 41.767 | 249 | 119 | 9 | 1 | 236 | 1 | 236 | 2.16E-41 | 152 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023451.01.T01 | G7LD36 | 85.774 | 239 | 28 | 3 | 1 | 236 | 1 | 236 | 3.29e-134 | 397 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048058.01 | MsG0480023451.01 | 0.801343 | 9.415038e-49 | 4.485568e-46 |
| MsG0080048461.01 | MsG0480023451.01 | 0.824070 | 9.851957e-54 | 8.635386e-51 |
| MsG0180000116.01 | MsG0480023451.01 | 0.800593 | 1.340288e-48 | 6.265188e-46 |
| MsG0180000197.01 | MsG0480023451.01 | 0.844750 | 6.229050e-59 | 1.015608e-55 |
| MsG0180000295.01 | MsG0480023451.01 | 0.824957 | 6.090820e-54 | 5.476488e-51 |
| MsG0180000402.01 | MsG0480023451.01 | 0.832797 | 7.700258e-56 | 8.699127e-53 |
| MsG0180000555.01 | MsG0480023451.01 | 0.844393 | 7.774365e-59 | 1.253252e-55 |
| MsG0180000585.01 | MsG0480023451.01 | 0.814615 | 1.407419e-51 | 9.489063e-49 |
| MsG0180000741.01 | MsG0480023451.01 | 0.805857 | 1.090041e-49 | 5.829628e-47 |
| MsG0180000821.01 | MsG0480023451.01 | 0.817147 | 3.833622e-52 | 2.769703e-49 |
| MsG0180000822.01 | MsG0480023451.01 | 0.800755 | 1.241662e-48 | 5.828112e-46 |
| MsG0180001566.01 | MsG0480023451.01 | 0.804627 | 1.972985e-49 | 1.022209e-46 |
| MsG0180001775.01 | MsG0480023451.01 | 0.815530 | 8.817807e-52 | 6.094941e-49 |
| MsG0180001854.01 | MsG0480023451.01 | 0.869235 | 3.603159e-66 | 1.342280e-62 |
| MsG0180002273.01 | MsG0480023451.01 | 0.816254 | 6.079633e-52 | 4.286485e-49 |
| MsG0180002952.01 | MsG0480023451.01 | 0.804954 | 1.685501e-49 | 8.807497e-47 |
| MsG0180004222.01 | MsG0480023451.01 | 0.800327 | 1.518056e-48 | 7.048887e-46 |
| MsG0180004357.01 | MsG0480023451.01 | 0.809664 | 1.692179e-50 | 9.995560e-48 |
| MsG0180004486.01 | MsG0480023451.01 | 0.830670 | 2.577137e-55 | 2.733835e-52 |
| MsG0180004689.01 | MsG0480023451.01 | 0.803175 | 3.951807e-49 | 1.972533e-46 |
| MsG0180005443.01 | MsG0480023451.01 | 0.806268 | 8.934000e-50 | 4.828371e-47 |
| MsG0180005445.01 | MsG0480023451.01 | 0.812449 | 4.215338e-51 | 2.681191e-48 |
| MsG0180005451.01 | MsG0480023451.01 | 0.804207 | 2.412847e-49 | 1.236684e-46 |
| MsG0180005493.01 | MsG0480023451.01 | 0.826560 | 2.536805e-54 | 2.387280e-51 |
| MsG0180005507.01 | MsG0480023451.01 | 0.869443 | 3.084425e-66 | 1.157952e-62 |
| MsG0180005708.01 | MsG0480023451.01 | 0.811560 | 6.586232e-51 | 4.090063e-48 |
| MsG0180005921.01 | MsG0480023451.01 | 0.801637 | 8.197521e-49 | 3.934422e-46 |
| MsG0180006061.01 | MsG0480023451.01 | 0.825263 | 5.156612e-54 | 4.676924e-51 |
| MsG0280010614.01 | MsG0480023451.01 | 0.818182 | 2.239697e-52 | 1.664718e-49 |
| MsG0280010816.01 | MsG0480023451.01 | 0.838715 | 2.438759e-57 | 3.294411e-54 |
| MsG0280011105.01 | MsG0480023451.01 | 0.858171 | 9.891169e-63 | 2.501958e-59 |
| MsG0280011258.01 | MsG0480023451.01 | 0.808739 | 2.671163e-50 | 1.539250e-47 |
| MsG0280011334.01 | MsG0480023451.01 | 0.843713 | 1.183023e-58 | 1.866983e-55 |
| MsG0280011410.01 | MsG0480023451.01 | 0.810245 | 1.268541e-50 | 7.607908e-48 |
| MsG0380011663.01 | MsG0480023451.01 | 0.803880 | 2.822491e-49 | 1.434620e-46 |
| MsG0380012033.01 | MsG0480023451.01 | 0.803732 | 3.028681e-49 | 1.533548e-46 |
| MsG0380012057.01 | MsG0480023451.01 | 0.819003 | 1.458729e-52 | 1.109208e-49 |
| MsG0380014118.01 | MsG0480023451.01 | 0.802431 | 5.629974e-49 | 2.757415e-46 |
| MsG0380014701.01 | MsG0480023451.01 | 0.800867 | 1.178077e-48 | 5.545573e-46 |
| MsG0380014903.01 | MsG0480023451.01 | 0.807486 | 4.938170e-50 | 2.753921e-47 |
| MsG0480018640.01 | MsG0480023451.01 | 0.802979 | 4.337562e-49 | 2.154153e-46 |
| MsG0480018846.01 | MsG0480023451.01 | 0.826998 | 1.993514e-54 | 1.899984e-51 |
| MsG0480020242.01 | MsG0480023451.01 | 0.864529 | 1.139006e-64 | 3.587357e-61 |
| MsG0480021778.01 | MsG0480023451.01 | 0.800054 | 1.725587e-48 | 7.956781e-46 |
| MsG0480021784.01 | MsG0480023451.01 | 0.807438 | 5.054975e-50 | 2.815508e-47 |
| MsG0480021870.01 | MsG0480023451.01 | 0.826575 | 2.515839e-54 | 2.368533e-51 |
| MsG0480021900.01 | MsG0480023451.01 | 0.867305 | 1.509524e-65 | 5.245699e-62 |
| MsG0480022094.01 | MsG0480023451.01 | 0.810565 | 1.082219e-50 | 6.545481e-48 |
| MsG0480022115.01 | MsG0480023451.01 | 0.807693 | 4.462522e-50 | 2.501981e-47 |
| MsG0480022228.01 | MsG0480023451.01 | 0.813663 | 2.283500e-51 | 1.500374e-48 |
| MsG0480022406.01 | MsG0480023451.01 | 0.824637 | 7.246197e-54 | 6.456106e-51 |
| MsG0480022451.01 | MsG0480023451.01 | 0.822340 | 2.496407e-53 | 2.083739e-50 |
| MsG0480022597.01 | MsG0480023451.01 | 0.856393 | 3.316630e-62 | 7.904672e-59 |
| MsG0480022598.01 | MsG0480023451.01 | 0.872869 | 2.282206e-67 | 9.700068e-64 |
| MsG0480022738.01 | MsG0480023451.01 | 0.822581 | 2.193959e-53 | 1.844023e-50 |
| MsG0480023421.01 | MsG0480023451.01 | 0.810997 | 8.725418e-51 | 5.339274e-48 |
| MsG0480023451.01 | MsG0480023670.01 | 0.865794 | 4.561494e-65 | 1.502932e-61 |
| MsG0480023451.01 | MsG0480023718.01 | 0.900720 | 5.525163e-78 | 7.232977e-74 |
| MsG0480023451.01 | MsG0480023756.01 | 0.815142 | 1.075705e-51 | 7.356061e-49 |
| MsG0480023451.01 | MsG0480024010.01 | 0.843045 | 1.783365e-58 | 2.755529e-55 |
| MsG0480023451.01 | MsG0580024157.01 | 0.877326 | 6.872181e-69 | 3.441875e-65 |
| MsG0480023451.01 | MsG0580024205.01 | 0.802610 | 5.169388e-49 | 2.543337e-46 |
| MsG0480023451.01 | MsG0580024418.01 | 0.818620 | 1.782151e-52 | 1.340667e-49 |
| MsG0480023451.01 | MsG0580024477.01 | 0.821444 | 4.025070e-53 | 3.275128e-50 |
| MsG0480023451.01 | MsG0580024682.01 | 0.801474 | 8.850975e-49 | 4.230803e-46 |
| MsG0480023451.01 | MsG0580024960.01 | 0.836278 | 1.027595e-56 | 1.288973e-53 |
| MsG0480023451.01 | MsG0580025008.01 | 0.829750 | 4.321471e-55 | 4.463963e-52 |
| MsG0480023451.01 | MsG0580025025.01 | 0.809149 | 2.182165e-50 | 1.271397e-47 |
| MsG0480023451.01 | MsG0580025206.01 | 0.840395 | 8.922628e-58 | 1.269332e-54 |
| MsG0480023451.01 | MsG0580025532.01 | 0.836753 | 7.779069e-57 | 9.896875e-54 |
| MsG0480023451.01 | MsG0580025555.01 | 0.848271 | 6.818077e-60 | 1.244453e-56 |
| MsG0480023451.01 | MsG0580025900.01 | 0.827579 | 1.447137e-54 | 1.402674e-51 |
| MsG0480023451.01 | MsG0580027208.01 | 0.803108 | 4.080544e-49 | 2.033156e-46 |
| MsG0480023451.01 | MsG0580027659.01 | 0.803773 | 2.969923e-49 | 1.505376e-46 |
| MsG0480023451.01 | MsG0580027748.01 | 0.800295 | 1.540813e-48 | 7.148768e-46 |
| MsG0480023451.01 | MsG0580027827.01 | 0.806109 | 9.649423e-50 | 5.193724e-47 |
| MsG0480023451.01 | MsG0580027832.01 | 0.800608 | 1.330796e-48 | 6.223328e-46 |
| MsG0480023451.01 | MsG0580028517.01 | 0.800056 | 1.724061e-48 | 7.950131e-46 |
| MsG0480023451.01 | MsG0580029198.01 | 0.815847 | 7.494154e-52 | 5.224793e-49 |
| MsG0480023451.01 | MsG0580029592.01 | 0.812499 | 4.110593e-51 | 2.617879e-48 |
| MsG0480023451.01 | MsG0680030447.01 | 0.815705 | 8.059942e-52 | 5.597986e-49 |
| MsG0480023451.01 | MsG0680030677.01 | 0.819761 | 9.799982e-53 | 7.609932e-50 |
| MsG0480023451.01 | MsG0680030684.01 | 0.803289 | 3.742394e-49 | 1.873397e-46 |
| MsG0480023451.01 | MsG0680030685.01 | 0.803060 | 4.174277e-49 | 2.077308e-46 |
| MsG0480023451.01 | MsG0680032831.01 | 0.820078 | 8.295067e-53 | 6.498850e-50 |
| MsG0480023451.01 | MsG0680034757.01 | 0.808496 | 3.010358e-50 | 1.723870e-47 |
| MsG0480023451.01 | MsG0680034843.01 | 0.802183 | 6.332173e-49 | 3.081613e-46 |
| MsG0480023451.01 | MsG0680035656.01 | 0.834786 | 2.450843e-56 | 2.938517e-53 |
| MsG0480023451.01 | MsG0680035716.01 | 0.812845 | 3.453032e-51 | 2.219021e-48 |
| MsG0480023451.01 | MsG0780036548.01 | 0.800153 | 1.647050e-48 | 7.613669e-46 |
| MsG0480023451.01 | MsG0780036630.01 | 0.848185 | 7.201522e-60 | 1.310662e-56 |
| MsG0480023451.01 | MsG0780036635.01 | 0.817966 | 2.506788e-52 | 1.852124e-49 |
| MsG0480023451.01 | MsG0780036773.01 | 0.827603 | 1.428121e-54 | 1.385203e-51 |
| MsG0480023451.01 | MsG0780036950.01 | 0.800175 | 1.629945e-48 | 7.538776e-46 |
| MsG0480023451.01 | MsG0780037123.01 | 0.814476 | 1.511328e-51 | 1.015164e-48 |
| MsG0480023451.01 | MsG0780037146.01 | 0.807891 | 4.049659e-50 | 2.282270e-47 |
| MsG0480023451.01 | MsG0780037630.01 | 0.803248 | 3.817283e-49 | 1.908891e-46 |
| MsG0480023451.01 | MsG0780037975.01 | 0.848628 | 5.428489e-60 | 1.002291e-56 |
| MsG0480023451.01 | MsG0780038113.01 | 0.802932 | 4.437481e-49 | 2.201153e-46 |
| MsG0480023451.01 | MsG0780038117.01 | 0.838264 | 3.187780e-57 | 4.246773e-54 |
| MsG0480023451.01 | MsG0780038386.01 | 0.831033 | 2.099897e-55 | 2.251403e-52 |
| MsG0480023451.01 | MsG0780038602.01 | 0.808268 | 3.366979e-50 | 1.916666e-47 |
| MsG0480023451.01 | MsG0780038644.01 | 0.800146 | 1.652888e-48 | 7.639273e-46 |
| MsG0480023451.01 | MsG0780038829.01 | 0.803230 | 3.850000e-49 | 1.924340e-46 |
| MsG0480023451.01 | MsG0780038937.01 | 0.825243 | 5.213508e-54 | 4.725822e-51 |
| MsG0480023451.01 | MsG0780039654.01 | 0.825330 | 4.972791e-54 | 4.518871e-51 |
| MsG0480023451.01 | MsG0780039655.01 | 0.862403 | 5.194632e-64 | 1.519421e-60 |
| MsG0480023451.01 | MsG0780039712.01 | 0.802306 | 5.973772e-49 | 2.916575e-46 |
| MsG0480023451.01 | MsG0780040047.01 | 0.811335 | 7.370504e-51 | 4.549771e-48 |
| MsG0480023451.01 | MsG0780040176.01 | 0.818228 | 2.187407e-52 | 1.627796e-49 |
| MsG0480023451.01 | MsG0780040389.01 | 0.802541 | 5.342243e-49 | 2.623755e-46 |
| MsG0480023451.01 | MsG0780040488.01 | 0.807307 | 5.388794e-50 | 2.991350e-47 |
| MsG0480023451.01 | MsG0780040516.01 | 0.818539 | 1.859772e-52 | 1.395996e-49 |
| MsG0480023451.01 | MsG0780040788.01 | 0.807718 | 4.408058e-50 | 2.473185e-47 |
| MsG0480023451.01 | MsG0780040947.01 | 0.814671 | 1.368058e-51 | 9.237419e-49 |
| MsG0480023451.01 | MsG0780041764.01 | 0.808142 | 3.581588e-50 | 2.032020e-47 |
| MsG0480023451.01 | MsG0780041787.01 | 0.860475 | 2.013142e-63 | 5.510019e-60 |
| MsG0480023451.01 | MsG0880042547.01 | 0.821787 | 3.352903e-53 | 2.754833e-50 |
| MsG0480023451.01 | MsG0880043489.01 | 0.854610 | 1.097923e-61 | 2.465981e-58 |
| MsG0480023451.01 | MsG0880044075.01 | 0.812608 | 3.891983e-51 | 2.485664e-48 |
| MsG0480023451.01 | MsG0880045185.01 | 0.803414 | 3.526548e-49 | 1.771061e-46 |
| MsG0480023451.01 | MsG0880045372.01 | 0.802278 | 6.052756e-49 | 2.952906e-46 |
| MsG0480023451.01 | MsG0880045639.01 | 0.873829 | 1.085673e-67 | 4.778298e-64 |
| MsG0480023451.01 | MsG0880045782.01 | 0.817490 | 3.210545e-52 | 2.341454e-49 |
| MsG0480023451.01 | MsG0880045914.01 | 0.833660 | 4.694860e-56 | 5.442566e-53 |
| MsG0480023451.01 | MsG0880046027.01 | 0.802545 | 5.332669e-49 | 2.619304e-46 |
| MsG0480023451.01 | MsG0880046264.01 | 0.817589 | 3.049652e-52 | 2.230045e-49 |
| MsG0480023451.01 | MsG0880046560.01 | 0.867679 | 1.145675e-65 | 4.034047e-62 |
| MsG0480023451.01 | MsG0880046561.01 | 0.825977 | 3.492115e-54 | 3.232377e-51 |
| MsG0480023451.01 | MsG0880046762.01 | 0.814405 | 1.566591e-51 | 1.050284e-48 |
| MsG0480023451.01 | MsG0880046967.01 | 0.808320 | 3.282560e-50 | 1.871141e-47 |
| MsG0480023451.01 | MsG0880047337.01 | 0.848459 | 6.049071e-60 | 1.110929e-56 |
| MsG0380015289.01 | MsG0480023451.01 | 0.821479 | 3.950022e-53 | 3.217229e-50 |
| MsG0380015702.01 | MsG0480023451.01 | 0.842349 | 2.730632e-58 | 4.128921e-55 |
| MsG0380015878.01 | MsG0480023451.01 | 0.834294 | 3.258003e-56 | 3.849903e-53 |
| MsG0380016510.01 | MsG0480023451.01 | 0.813167 | 2.935355e-51 | 1.902686e-48 |
| MsG0380017055.01 | MsG0480023451.01 | 0.844994 | 5.355701e-59 | 8.799162e-56 |
| MsG0380017177.01 | MsG0480023451.01 | 0.827148 | 1.835524e-54 | 1.757044e-51 |
| MsG0380017232.01 | MsG0480023451.01 | 0.801431 | 9.033275e-49 | 4.313181e-46 |
| MsG0380017233.01 | MsG0480023451.01 | 0.815339 | 9.724505e-52 | 6.686490e-49 |
| MsG0380017266.01 | MsG0480023451.01 | -0.818943 | 1.505481e-52 | 1.142872e-49 |
| MsG0380017631.01 | MsG0480023451.01 | 0.818056 | 2.391516e-52 | 1.771335e-49 |
| MsG0280006692.01 | MsG0480023451.01 | 0.890755 | 7.393682e-74 | 6.302257e-70 |
| MsG0280007048.01 | MsG0480023451.01 | 0.815618 | 8.429696e-52 | 5.841202e-49 |
| MsG0280007208.01 | MsG0480023451.01 | 0.805395 | 1.363030e-49 | 7.202995e-47 |
| MsG0280007481.01 | MsG0480023451.01 | 0.844980 | 5.402023e-59 | 8.872094e-56 |
| MsG0280007484.01 | MsG0480023451.01 | 0.854738 | 1.007757e-61 | 2.273055e-58 |
| MsG0280007740.01 | MsG0480023451.01 | 0.833507 | 5.124713e-56 | 5.913922e-53 |
| MsG0280007764.01 | MsG0480023451.01 | 0.820054 | 8.397944e-53 | 6.575108e-50 |
| MsG0280008039.01 | MsG0480023451.01 | 0.814680 | 1.362075e-51 | 9.199114e-49 |
| MsG0280008270.01 | MsG0480023451.01 | 0.840044 | 1.102040e-57 | 1.550963e-54 |
| MsG0280008363.01 | MsG0480023451.01 | 0.807312 | 5.375561e-50 | 2.984387e-47 |
| MsG0280008615.01 | MsG0480023451.01 | 0.803723 | 3.042587e-49 | 1.540182e-46 |
| MsG0280009417.01 | MsG0480023451.01 | 0.820055 | 8.394776e-53 | 6.572726e-50 |
| MsG0280010170.01 | MsG0480023451.01 | 0.801278 | 9.710241e-49 | 4.618640e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023451.01.T01 | MTR_8g092870 | 85.774 | 239 | 28 | 3 | 1 | 236 | 1 | 236 | 8.34e-138 | 397 |
| MsG0480023451.01.T01 | MTR_3g095730 | 51.429 | 245 | 101 | 7 | 1 | 235 | 1 | 237 | 4.90e-69 | 221 |
| MsG0480023451.01.T01 | MTR_5g077510 | 34.507 | 142 | 78 | 7 | 37 | 169 | 62 | 197 | 5.45e-12 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023451.01.T01 | AT5G64660 | 41.767 | 249 | 119 | 9 | 1 | 236 | 1 | 236 | 2.20e-42 | 152 |
| MsG0480023451.01.T01 | AT3G18710 | 39.526 | 253 | 131 | 6 | 13 | 255 | 9 | 249 | 3.17e-42 | 151 |
| MsG0480023451.01.T01 | AT5G09800 | 43.284 | 201 | 102 | 4 | 37 | 235 | 38 | 228 | 1.35e-35 | 133 |
Find 76 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACCGTTCCGAGTTTATTC+CGG | 0.314022 | 4:+85305021 | MsG0480023451.01.T01:CDS |
| CATGGGACGTCGAGGGAAAT+AGG | 0.334835 | 4:-85305047 | None:intergenic |
| GGAGGGATTCGTGTGCGTTC+TGG | 0.338842 | 4:+85305417 | MsG0480023451.01.T01:CDS |
| ACGAAAGTGCGTTCTCCATT+AGG | 0.348641 | 4:-85305757 | None:intergenic |
| GAAACGGAACAAGGCGAAAC+TGG | 0.354390 | 4:+85305802 | MsG0480023451.01.T01:CDS |
| GGTTGTTATTGCGTTGGATT+TGG | 0.360751 | 4:+85305498 | MsG0480023451.01.T01:CDS |
| AACTCGGTGACGCCGTTAGA+AGG | 0.386589 | 4:-85305856 | None:intergenic |
| GGAGAACGCACTTTCGTGTT+TGG | 0.389136 | 4:+85305763 | MsG0480023451.01.T01:CDS |
| TCGGTGTATAACGGAGATTC+CGG | 0.396983 | 4:-85305256 | None:intergenic |
| GCACACGAATCCCTCCGTTT+TGG | 0.409841 | 4:-85305410 | None:intergenic |
| ATCTCCGGGGTCTCTGCAAC+CGG | 0.417635 | 4:+85305071 | MsG0480023451.01.T01:CDS |
| AGCGCCGTCGTGTGTGGTGG+CGG | 0.420683 | 4:+85305961 | MsG0480023451.01.T01:CDS |
| CTCGACGTCCCATGAATCTC+CGG | 0.421623 | 4:+85305056 | MsG0480023451.01.T01:CDS |
| CCAAGGATTTCGTTCCGAAC+CGG | 0.425782 | 4:+85305176 | MsG0480023451.01.T01:CDS |
| GAACCGAATCGGACCAGATC+TGG | 0.426727 | 4:-85305216 | None:intergenic |
| GCAGAGACCCCGGAGATTCA+TGG | 0.428745 | 4:-85305065 | None:intergenic |
| GACTCGGTGGCGAACCGAAT+CGG | 0.451818 | 4:-85305227 | None:intergenic |
| TTGAGTCAGGTAGTGAGAAC+CGG | 0.463717 | 4:+85305323 | MsG0480023451.01.T01:CDS |
| GAGTGTGGAATTGCTTGAAC+AGG | 0.467131 | 4:+85305477 | MsG0480023451.01.T01:CDS |
| TCGACGTCCCATGAATCTCC+GGG | 0.467656 | 4:+85305057 | MsG0480023451.01.T01:CDS |
| CATCCAGATCTGGTCCGATT+CGG | 0.468264 | 4:+85305213 | MsG0480023451.01.T01:CDS |
| ATCCAACGCTGGCTCGATAA+CGG | 0.470884 | 4:+85305115 | MsG0480023451.01.T01:CDS |
| ATTAGGTTCTGATCTTTCTC+CGG | 0.473675 | 4:-85305740 | None:intergenic |
| CCGGTTCGGAACGAAATCCT+TGG | 0.487110 | 4:-85305176 | None:intergenic |
| AGCCTCTGTAACGTCCGGTT+CGG | 0.488322 | 4:-85305190 | None:intergenic |
| GGAGAAAGATCAGAACCTAA+TGG | 0.501306 | 4:+85305742 | MsG0480023451.01.T01:CDS |
| TACAGAGGCTCATCCAGATC+TGG | 0.511051 | 4:+85305203 | MsG0480023451.01.T01:CDS |
| TAGCAATCTCAACGTCGAAA+CGG | 0.512266 | 4:+85305786 | MsG0480023451.01.T01:CDS |
| GGTGTTGAAACTTATAGAAA+CGG | 0.512499 | 4:+85305901 | MsG0480023451.01.T01:CDS |
| ACGGTGTCGTCGACAAAGGA+AGG | 0.515605 | 4:+85305920 | MsG0480023451.01.T01:CDS |
| TACCGTTATCGAGCCAGCGT+TGG | 0.518220 | 4:-85305117 | None:intergenic |
| TTTCTTGATAATGTTGATGG+AGG | 0.529867 | 4:+85305445 | MsG0480023451.01.T01:CDS |
| CTCAACGTCGAAACGGAACA+AGG | 0.536307 | 4:+85305793 | MsG0480023451.01.T01:CDS |
| AGAAACGGTGTCGTCGACAA+AGG | 0.541994 | 4:+85305916 | MsG0480023451.01.T01:CDS |
| CAGAGACCCCGGAGATTCAT+GGG | 0.542388 | 4:-85305064 | None:intergenic |
| GGATGAGCCTCTGTAACGTC+CGG | 0.544595 | 4:-85305195 | None:intergenic |
| TCAACAGACTCGGTGTATAA+CGG | 0.556543 | 4:-85305265 | None:intergenic |
| TGAACAGGTTGTTATTGCGT+TGG | 0.556947 | 4:+85305492 | MsG0480023451.01.T01:CDS |
| TGAAGAATCTGCATAGTGGC+GGG | 0.558784 | 4:-85305151 | None:intergenic |
| AGTCGCTTGTGAAAATTGTA+CGG | 0.565379 | 4:+85305350 | MsG0480023451.01.T01:CDS |
| GAGATTCCGGCGAGTCGACT+CGG | 0.566820 | 4:-85305243 | None:intergenic |
| GGTCTTCTCGAACCTTCTAA+CGG | 0.571682 | 4:+85305844 | MsG0480023451.01.T01:CDS |
| TCTGCATAGTGGCGGGACAA+AGG | 0.573420 | 4:-85305144 | None:intergenic |
| GAGTTTATGTGTTTCTGTGA+CGG | 0.574001 | 4:+85305874 | MsG0480023451.01.T01:CDS |
| GGTGTTGAAATTCATAGCGG+TGG | 0.581421 | 4:+85305651 | MsG0480023451.01.T01:CDS |
| ATGCGAAGCGCCGTCGTGTG+TGG | 0.582842 | 4:+85305955 | MsG0480023451.01.T01:CDS |
| GTCGACAAAGGAAGGGAGGT+CGG | 0.586477 | 4:+85305928 | MsG0480023451.01.T01:CDS |
| TGGAGGAGTTGAACAGAGTG+TGG | 0.586843 | 4:+85305462 | MsG0480023451.01.T01:CDS |
| CGAAGCGCCGTCGTGTGTGG+TGG | 0.591962 | 4:+85305958 | MsG0480023451.01.T01:CDS |
| GGTTCATCTCGGAGCAGTGA+AGG | 0.592190 | 4:+85305823 | MsG0480023451.01.T01:CDS |
| AAGGCGAAACTGGTTCATCT+CGG | 0.598473 | 4:+85305812 | MsG0480023451.01.T01:CDS |
| TCGCCACCGAGTCGACTCGC+CGG | 0.598739 | 4:+85305237 | MsG0480023451.01.T01:CDS |
| GGTTTGAAGAATCTGCATAG+TGG | 0.603561 | 4:-85305155 | None:intergenic |
| CGGAGATTCATGGGACGTCG+AGG | 0.606472 | 4:-85305055 | None:intergenic |
| AACTCCGGTTGCAGAGACCC+CGG | 0.607373 | 4:-85305075 | None:intergenic |
| ATGTGTTTCTGTGACGGAGA+AGG | 0.608428 | 4:+85305880 | MsG0480023451.01.T01:CDS |
| TTGATTCTTGTTCTTCAACA+AGG | 0.611383 | 4:+85305598 | MsG0480023451.01.T01:CDS |
| TTGAAGAATCTGCATAGTGG+CGG | 0.623643 | 4:-85305152 | None:intergenic |
| TTGAACGTCGTAGGTAACTC+CGG | 0.632151 | 4:-85305090 | None:intergenic |
| TATGCAGATTCTTCAAACCA+AGG | 0.633645 | 4:+85305159 | MsG0480023451.01.T01:CDS |
| TTCCGAACCGGACGTTACAG+AGG | 0.635065 | 4:+85305188 | MsG0480023451.01.T01:CDS |
| GGAGATTCATGGGACGTCGA+GGG | 0.635081 | 4:-85305054 | None:intergenic |
| ATAGGACACCGGAATAAACT+CGG | 0.640762 | 4:-85305029 | None:intergenic |
| ATCACGTCTATCAACAGACT+CGG | 0.644157 | 4:-85305275 | None:intergenic |
| GACGTTCAAGTATCCAACGC+TGG | 0.651960 | 4:+85305104 | MsG0480023451.01.T01:CDS |
| GTCACAGAAACACATAAACT+CGG | 0.652220 | 4:-85305872 | None:intergenic |
| ATCGCCGCCACCACACACGA+CGG | 0.660437 | 4:-85305965 | None:intergenic |
| CGGTGTCGTCGACAAAGGAA+GGG | 0.661079 | 4:+85305921 | MsG0480023451.01.T01:CDS |
| GTTGGATACTTGAACGTCGT+AGG | 0.669626 | 4:-85305099 | None:intergenic |
| ACACCGGAATAAACTCGGAA+CGG | 0.672519 | 4:-85305024 | None:intergenic |
| CGACGTCCCATGAATCTCCG+GGG | 0.694002 | 4:+85305058 | MsG0480023451.01.T01:CDS |
| ATTCCGGCGAGTCGACTCGG+TGG | 0.696812 | 4:-85305240 | None:intergenic |
| TGTCGTCGACAAAGGAAGGG+AGG | 0.709533 | 4:+85305924 | MsG0480023451.01.T01:CDS |
| ATTGCTCAATCAAATCACGC+CGG | 0.719116 | 4:+85305721 | MsG0480023451.01.T01:CDS |
| CGTCGAGGGAAATAGGACAC+CGG | 0.726868 | 4:-85305040 | None:intergenic |
| TAGGGTGTTGAAATTCATAG+CGG | 0.751361 | 4:+85305648 | MsG0480023451.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTTTCTTGATAATGTTGA+TGG | + | Chr4:85305442-85305461 | MsG0480023451.01.T01:CDS | 25.0% |
| TTGATTCTTGTTCTTCAACA+AGG | + | Chr4:85305598-85305617 | MsG0480023451.01.T01:CDS | 30.0% | |
| TTGCGAATCAAAAATTGCAT+CGG | + | Chr4:85305624-85305643 | MsG0480023451.01.T01:CDS | 30.0% | |
| ! | GGTGTTGAAACTTATAGAAA+CGG | + | Chr4:85305901-85305920 | MsG0480023451.01.T01:CDS | 30.0% |
| ! | TGAAGAGAATTGTGTTTTTC+TGG | + | Chr4:85305387-85305406 | MsG0480023451.01.T01:CDS | 30.0% |
| !! | AATCAAAAATTGCATCGGTT+AGG | + | Chr4:85305629-85305648 | MsG0480023451.01.T01:CDS | 30.0% |
| !! | ATCAAAAATTGCATCGGTTA+GGG | + | Chr4:85305630-85305649 | MsG0480023451.01.T01:CDS | 30.0% |
| !! | TTTCTTGATAATGTTGATGG+AGG | + | Chr4:85305445-85305464 | MsG0480023451.01.T01:CDS | 30.0% |
| AGCTTCTAGTAGCTGAAAAA+AGG | + | Chr4:85305686-85305705 | MsG0480023451.01.T01:CDS | 35.0% | |
| AGTCGCTTGTGAAAATTGTA+CGG | + | Chr4:85305350-85305369 | MsG0480023451.01.T01:CDS | 35.0% | |
| GTCACAGAAACACATAAACT+CGG | - | Chr4:85305875-85305894 | None:intergenic | 35.0% | |
| TATGCAGATTCTTCAAACCA+AGG | + | Chr4:85305159-85305178 | MsG0480023451.01.T01:CDS | 35.0% | |
| ! | ATTAGGTTCTGATCTTTCTC+CGG | - | Chr4:85305743-85305762 | None:intergenic | 35.0% |
| ! | GAGTTTATGTGTTTCTGTGA+CGG | + | Chr4:85305874-85305893 | MsG0480023451.01.T01:CDS | 35.0% |
| !! | ATCGATTTTGAAGAGCAAGA+AGG | + | Chr4:85305546-85305565 | MsG0480023451.01.T01:CDS | 35.0% |
| !! | TAGGGTGTTGAAATTCATAG+CGG | + | Chr4:85305648-85305667 | MsG0480023451.01.T01:CDS | 35.0% |
| !! | TTGTGTTTTTCTGGCCAAAA+CGG | + | Chr4:85305396-85305415 | MsG0480023451.01.T01:CDS | 35.0% |
| ATAGGACACCGGAATAAACT+CGG | - | Chr4:85305032-85305051 | None:intergenic | 40.0% | |
| ATCACGTCTATCAACAGACT+CGG | - | Chr4:85305278-85305297 | None:intergenic | 40.0% | |
| ATTGCTCAATCAAATCACGC+CGG | + | Chr4:85305721-85305740 | MsG0480023451.01.T01:CDS | 40.0% | |
| GGAGAAAGATCAGAACCTAA+TGG | + | Chr4:85305742-85305761 | MsG0480023451.01.T01:CDS | 40.0% | |
| GGTTTGAAGAATCTGCATAG+TGG | - | Chr4:85305158-85305177 | None:intergenic | 40.0% | |
| TAGCAATCTCAACGTCGAAA+CGG | + | Chr4:85305786-85305805 | MsG0480023451.01.T01:CDS | 40.0% | |
| TTGAAGAATCTGCATAGTGG+CGG | - | Chr4:85305155-85305174 | None:intergenic | 40.0% | |
| ! | GGTTGTTATTGCGTTGGATT+TGG | + | Chr4:85305498-85305517 | MsG0480023451.01.T01:CDS | 40.0% |
| ! | TGAACAGGTTGTTATTGCGT+TGG | + | Chr4:85305492-85305511 | MsG0480023451.01.T01:CDS | 40.0% |
| !! | TCAACAGACTCGGTGTATAA+CGG | - | Chr4:85305268-85305287 | None:intergenic | 40.0% |
| !!! | GCTATCACTGATTTTGAGTC+AGG | + | Chr4:85305310-85305329 | MsG0480023451.01.T01:CDS | 40.0% |
| ACACCGGAATAAACTCGGAA+CGG | - | Chr4:85305027-85305046 | None:intergenic | 45.0% | |
| CAACCGTTCCGAGTTTATTC+CGG | + | Chr4:85305021-85305040 | MsG0480023451.01.T01:CDS | 45.0% | |
| GAGTGTGGAATTGCTTGAAC+AGG | + | Chr4:85305477-85305496 | MsG0480023451.01.T01:CDS | 45.0% | |
| GGTCTTCTCGAACCTTCTAA+CGG | + | Chr4:85305844-85305863 | MsG0480023451.01.T01:CDS | 45.0% | |
| GTTGGATACTTGAACGTCGT+AGG | - | Chr4:85305102-85305121 | None:intergenic | 45.0% | |
| TGAAGAATCTGCATAGTGGC+GGG | - | Chr4:85305154-85305173 | None:intergenic | 45.0% | |
| TTGAACGTCGTAGGTAACTC+CGG | - | Chr4:85305093-85305112 | None:intergenic | 45.0% | |
| TTGAGTCAGGTAGTGAGAAC+CGG | + | Chr4:85305323-85305342 | MsG0480023451.01.T01:CDS | 45.0% | |
| ! | AAGGCGAAACTGGTTCATCT+CGG | + | Chr4:85305812-85305831 | MsG0480023451.01.T01:CDS | 45.0% |
| ! | ACGAAAGTGCGTTCTCCATT+AGG | - | Chr4:85305760-85305779 | None:intergenic | 45.0% |
| ! | ATGTGTTTCTGTGACGGAGA+AGG | + | Chr4:85305880-85305899 | MsG0480023451.01.T01:CDS | 45.0% |
| ! | GGTGTTGAAATTCATAGCGG+TGG | + | Chr4:85305651-85305670 | MsG0480023451.01.T01:CDS | 45.0% |
| ! | TCGGTGTATAACGGAGATTC+CGG | - | Chr4:85305259-85305278 | None:intergenic | 45.0% |
| ! | TTTTCACAAGCGACTCGAAC+CGG | - | Chr4:85305345-85305364 | None:intergenic | 45.0% |
| !! | GTTTTTCTGGCCAAAACGGA+GGG | + | Chr4:85305400-85305419 | MsG0480023451.01.T01:CDS | 45.0% |
| !! | TGTTTTTCTGGCCAAAACGG+AGG | + | Chr4:85305399-85305418 | MsG0480023451.01.T01:CDS | 45.0% |
| CATCCAGATCTGGTCCGATT+CGG | + | Chr4:85305213-85305232 | MsG0480023451.01.T01:CDS | 50.0% | |
| CCAAGGATTTCGTTCCGAAC+CGG | + | Chr4:85305176-85305195 | MsG0480023451.01.T01:CDS | 50.0% | |
| CTCAACGTCGAAACGGAACA+AGG | + | Chr4:85305793-85305812 | MsG0480023451.01.T01:CDS | 50.0% | |
| GAAACGGAACAAGGCGAAAC+TGG | + | Chr4:85305802-85305821 | MsG0480023451.01.T01:CDS | 50.0% | |
| GACGTTCAAGTATCCAACGC+TGG | + | Chr4:85305104-85305123 | MsG0480023451.01.T01:CDS | 50.0% | |
| GGAGAACGCACTTTCGTGTT+TGG | + | Chr4:85305763-85305782 | MsG0480023451.01.T01:CDS | 50.0% | |
| TACAGAGGCTCATCCAGATC+TGG | + | Chr4:85305203-85305222 | MsG0480023451.01.T01:CDS | 50.0% | |
| TGGAGGAGTTGAACAGAGTG+TGG | + | Chr4:85305462-85305481 | MsG0480023451.01.T01:CDS | 50.0% | |
| !! | AGAAACGGTGTCGTCGACAA+AGG | + | Chr4:85305916-85305935 | MsG0480023451.01.T01:CDS | 50.0% |
| !! | ATCCAACGCTGGCTCGATAA+CGG | + | Chr4:85305115-85305134 | MsG0480023451.01.T01:CDS | 50.0% |
| AGCCTCTGTAACGTCCGGTT+CGG | - | Chr4:85305193-85305212 | None:intergenic | 55.0% | |
| CAGAGACCCCGGAGATTCAT+GGG | - | Chr4:85305067-85305086 | None:intergenic | 55.0% | |
| CATGGGACGTCGAGGGAAAT+AGG | - | Chr4:85305050-85305069 | None:intergenic | 55.0% | |
| CGTCGAGGGAAATAGGACAC+CGG | - | Chr4:85305043-85305062 | None:intergenic | 55.0% | |
| CTCGACGTCCCATGAATCTC+CGG | + | Chr4:85305056-85305075 | MsG0480023451.01.T01:CDS | 55.0% | |
| GAACCGAATCGGACCAGATC+TGG | - | Chr4:85305219-85305238 | None:intergenic | 55.0% | |
| GGAGATTCATGGGACGTCGA+GGG | - | Chr4:85305057-85305076 | None:intergenic | 55.0% | |
| GGATGAGCCTCTGTAACGTC+CGG | - | Chr4:85305198-85305217 | None:intergenic | 55.0% | |
| GGTTCATCTCGGAGCAGTGA+AGG | + | Chr4:85305823-85305842 | MsG0480023451.01.T01:CDS | 55.0% | |
| GTCGACAAAGGAAGGGAGGT+CGG | + | Chr4:85305928-85305947 | MsG0480023451.01.T01:CDS | 55.0% | |
| TACCGTTATCGAGCCAGCGT+TGG | - | Chr4:85305120-85305139 | None:intergenic | 55.0% | |
| TCGACGTCCCATGAATCTCC+GGG | + | Chr4:85305057-85305076 | MsG0480023451.01.T01:CDS | 55.0% | |
| TGTCGTCGACAAAGGAAGGG+AGG | + | Chr4:85305924-85305943 | MsG0480023451.01.T01:CDS | 55.0% | |
| TTCCGAACCGGACGTTACAG+AGG | + | Chr4:85305188-85305207 | MsG0480023451.01.T01:CDS | 55.0% | |
| ! | AACTCGGTGACGCCGTTAGA+AGG | - | Chr4:85305859-85305878 | None:intergenic | 55.0% |
| ! | GCACACGAATCCCTCCGTTT+TGG | - | Chr4:85305413-85305432 | None:intergenic | 55.0% |
| ! | TCTGCATAGTGGCGGGACAA+AGG | - | Chr4:85305147-85305166 | None:intergenic | 55.0% |
| !! | ACGGTGTCGTCGACAAAGGA+AGG | + | Chr4:85305920-85305939 | MsG0480023451.01.T01:CDS | 55.0% |
| !! | CCGGTTCGGAACGAAATCCT+TGG | - | Chr4:85305179-85305198 | None:intergenic | 55.0% |
| !! | CGGTGTCGTCGACAAAGGAA+GGG | + | Chr4:85305921-85305940 | MsG0480023451.01.T01:CDS | 55.0% |
| AACTCCGGTTGCAGAGACCC+CGG | - | Chr4:85305078-85305097 | None:intergenic | 60.0% | |
| ATCTCCGGGGTCTCTGCAAC+CGG | + | Chr4:85305071-85305090 | MsG0480023451.01.T01:CDS | 60.0% | |
| CGACGTCCCATGAATCTCCG+GGG | + | Chr4:85305058-85305077 | MsG0480023451.01.T01:CDS | 60.0% | |
| CGGAGATTCATGGGACGTCG+AGG | - | Chr4:85305058-85305077 | None:intergenic | 60.0% | |
| GAGATTCCGGCGAGTCGACT+CGG | - | Chr4:85305246-85305265 | None:intergenic | 60.0% | |
| GCAGAGACCCCGGAGATTCA+TGG | - | Chr4:85305068-85305087 | None:intergenic | 60.0% | |
| GGAGGGATTCGTGTGCGTTC+TGG | + | Chr4:85305417-85305436 | MsG0480023451.01.T01:CDS | 60.0% | |
| !! | GACTCGGTGGCGAACCGAAT+CGG | - | Chr4:85305230-85305249 | None:intergenic | 60.0% |
| ATCGCCGCCACCACACACGA+CGG | - | Chr4:85305968-85305987 | None:intergenic | 65.0% | |
| !! | ATGCGAAGCGCCGTCGTGTG+TGG | + | Chr4:85305955-85305974 | MsG0480023451.01.T01:CDS | 65.0% |
| !! | ATTCCGGCGAGTCGACTCGG+TGG | - | Chr4:85305243-85305262 | None:intergenic | 65.0% |
| AGCGCCGTCGTGTGTGGTGG+CGG | + | Chr4:85305961-85305980 | MsG0480023451.01.T01:CDS | 70.0% | |
| !! | CGAAGCGCCGTCGTGTGTGG+TGG | + | Chr4:85305958-85305977 | MsG0480023451.01.T01:CDS | 70.0% |
| !! | TCGCCACCGAGTCGACTCGC+CGG | + | Chr4:85305237-85305256 | MsG0480023451.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 85304998 | 85305993 | 85304998 | ID=MsG0480023451.01;Name=MsG0480023451.01 |
| Chr4 | mRNA | 85304998 | 85305993 | 85304998 | ID=MsG0480023451.01.T01;Parent=MsG0480023451.01;Name=MsG0480023451.01.T01;_AED=0.24;_eAED=0.24;_QI=0|-1|0|1|-1|1|1|0|331 |
| Chr4 | exon | 85304998 | 85305993 | 85304998 | ID=MsG0480023451.01.T01:exon:2838;Parent=MsG0480023451.01.T01 |
| Chr4 | CDS | 85304998 | 85305993 | 85304998 | ID=MsG0480023451.01.T01:cds;Parent=MsG0480023451.01.T01 |
| Gene Sequence |
| Protein sequence |
