AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180002317.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 36 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTTGGGTCTTCAATAAT+CGG 0.326782 1:-36633772 None:intergenic
TAGTACCTTAACATTTCATT+TGG 0.348520 1:-36633787 None:intergenic
TTATCTCCTTGCTATATTGA+AGG 0.410951 1:-36633714 None:intergenic
AGTACCTTAACATTTCATTT+GGG 0.420726 1:-36633786 None:intergenic
CTTCTACGTATCAGTTAGAT+TGG 0.423908 1:+36632574 MsG0180002317.01.T01:exon
GGGTCTTCAATAATCGGACT+AGG 0.455946 1:-36633766 None:intergenic
AAGACCCAAATGAAATGTTA+AGG 0.462842 1:+36633782 MsG0180002317.01.T01:three_prime_UTR
CAATCTAACTGATACGTAGA+AGG 0.467504 1:-36632573 None:intergenic
CCCTCTCAAACCTCCGATGC+CGG 0.476944 1:+36632402 MsG0180002317.01.T01:exon
GCTTCTCGTATGGCCAAAGT+TGG 0.481940 1:+36633741 MsG0180002317.01.T01:three_prime_UTR
CTTAGCGCCGTCTCCGGCAT+CGG 0.487035 1:-36632415 None:intergenic
ATCGGACTAGGGCCCAACTT+TGG 0.488711 1:-36633754 None:intergenic
CGACTCTGAAGCAACGCAAA+CGG 0.501415 1:-36632519 None:intergenic
CGGAGATTGATGATGCTGAT+TGG 0.511466 1:+36632469 MsG0180002317.01.T01:CDS
TAAGCTCGGAGGTGGAGATC+CGG 0.518121 1:+36632449 MsG0180002317.01.T01:CDS
CCTCGTGATCTTAGATCAGT+TGG 0.519655 1:+36630813 MsG0180002317.01.T01:five_prime_UTR
ATCGAAGTTAAGCTCGGAGG+TGG 0.533414 1:+36632441 MsG0180002317.01.T01:CDS
GATTGGCGAAGAATTGCATA+TGG 0.534205 1:+36632486 MsG0180002317.01.T01:CDS
CAATCAGCATCATCAATCTC+CGG 0.538504 1:-36632468 None:intergenic
GATAACCTTAGCGCCGTCTC+CGG 0.547965 1:-36632421 None:intergenic
CTTCTCGTATGGCCAAAGTT+GGG 0.548461 1:+36633742 MsG0180002317.01.T01:three_prime_UTR
TTGCGTTGCTTCAGAGTCGC+CGG 0.548587 1:+36632523 MsG0180002317.01.T01:CDS
AGATAACATCGCTTCTCGTA+TGG 0.554244 1:+36633731 MsG0180002317.01.T01:three_prime_UTR
TCCGGCATCGGAGGTTTGAG+AGG 0.555047 1:-36632403 None:intergenic
GTGATCTTAGATCAGTTGGT+AGG 0.559754 1:+36630817 MsG0180002317.01.T01:five_prime_UTR
TGATCTTAGATCAGTTGGTA+GGG 0.573904 1:+36630818 MsG0180002317.01.T01:five_prime_UTR
GGTCTTCAATAATCGGACTA+GGG 0.584981 1:-36633765 None:intergenic
CGATGCCGGAGACGGCGCTA+AGG 0.585070 1:+36632416 MsG0180002317.01.T01:exon
AGCGCCGTCTCCGGCATCGG+AGG 0.594499 1:-36632412 None:intergenic
CAAACCTCCGATGCCGGAGA+CGG 0.600136 1:+36632408 MsG0180002317.01.T01:exon
CCGGCATCGGAGGTTTGAGA+GGG 0.600200 1:-36632402 None:intergenic
ACATAGAAGAAATCGCACTC+CGG 0.611098 1:-36632542 None:intergenic
AAGGTTATCGAAGTTAAGCT+CGG 0.635323 1:+36632435 MsG0180002317.01.T01:CDS
GCATGTCCTTCAATATAGCA+AGG 0.645616 1:+36633708 MsG0180002317.01.T01:three_prime_UTR
CCAACTGATCTAAGATCACG+AGG 0.671333 1:-36630813 None:intergenic
GTTATCGAAGTTAAGCTCGG+AGG 0.743966 1:+36632438 MsG0180002317.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAGAAATTATAATATTAG+AGG + Chr1:36633115-36633134 MsG0180002317.01.T01:intron 10.0%
!! TTAGAAATTATAATATTAGA+GGG + Chr1:36633116-36633135 MsG0180002317.01.T01:intron 10.0%
!!! TTTATATTTGTTTTACAATT+AGG + Chr1:36633680-36633699 MsG0180002317.01.T01:intron 10.0%
!! TCAATTTATCAAATTATTAC+CGG - Chr1:36632657-36632676 None:intergenic 15.0%
!!! ATTTCAATTTGAAATTCTTA+TGG + Chr1:36632753-36632772 MsG0180002317.01.T01:intron 15.0%
!!! GTTTTTGCTAATAAAATTTT+CGG - Chr1:36630915-36630934 None:intergenic 15.0%
!!! TTAATTTGCTAATTTGATTT+AGG + Chr1:36632986-36633005 MsG0180002317.01.T01:intron 15.0%
!!! TTTCAATTTGAAATTCTTAT+GGG + Chr1:36632754-36632773 MsG0180002317.01.T01:intron 15.0%
!!! TTTTTTATTCATCAAAGTTT+GGG + Chr1:36633507-36633526 MsG0180002317.01.T01:intron 15.0%
!!! TTTTTTTATTCATCAAAGTT+TGG + Chr1:36633506-36633525 MsG0180002317.01.T01:intron 15.0%
!! AACCCAAAATCTTATTATTA+CGG - Chr1:36633616-36633635 None:intergenic 20.0%
!! AATAAAATTTAGGTGTAACT+AGG + Chr1:36631958-36631977 MsG0180002317.01.T01:intron 20.0%
!! TTTCAATTATAATGTGTTGT+TGG + Chr1:36632944-36632963 MsG0180002317.01.T01:intron 20.0%
!!! AAAGGTATATTAGTAACTTT+GGG - Chr1:36631240-36631259 None:intergenic 20.0%
!!! AACCGTAATAATAAGATTTT+GGG + Chr1:36633611-36633630 MsG0180002317.01.T01:intron 20.0%
!!! AATACGTAATAGTTTTTTGT+GGG - Chr1:36631294-36631313 None:intergenic 20.0%
!!! ATAATGCTCTTTTTTTTTTG+TGG - Chr1:36630886-36630905 None:intergenic 20.0%
!!! ATATTTGTTTTACAATTAGG+TGG + Chr1:36633683-36633702 MsG0180002317.01.T01:intron 20.0%
!!! TAAAGGTATATTAGTAACTT+TGG - Chr1:36631241-36631260 None:intergenic 20.0%
!!! TAAATGATATTTTACTATGC+TGG + Chr1:36632685-36632704 MsG0180002317.01.T01:intron 20.0%
!!! TAACCGTAATAATAAGATTT+TGG + Chr1:36633610-36633629 MsG0180002317.01.T01:intron 20.0%
!!! TATTTGTTTTACAATTAGGT+GGG + Chr1:36633684-36633703 MsG0180002317.01.T01:intron 20.0%
!!! TTCAATTTGAAATTCTTATG+GGG + Chr1:36632755-36632774 MsG0180002317.01.T01:intron 20.0%
!!! TTCGCATTTGATTATAATTT+TGG - Chr1:36632013-36632032 None:intergenic 20.0%
! AAAAATTAAAACCAGAGATC+AGG - Chr1:36633489-36633508 None:intergenic 25.0%
! AAGGTATATTAGTAACTTTG+GGG - Chr1:36631239-36631258 None:intergenic 25.0%
! AGATATAGACATTAATGAGT+TGG + Chr1:36633301-36633320 MsG0180002317.01.T01:intron 25.0%
! AGTACCTTAACATTTCATTT+GGG - Chr1:36633789-36633808 None:intergenic 25.0%
! ATTATGTAACTCAGTTTATG+TGG - Chr1:36631483-36631502 None:intergenic 25.0%
! GTTTACGTACATATTCTAAA+AGG - Chr1:36631560-36631579 None:intergenic 25.0%
! TAGTACCTTAACATTTCATT+TGG - Chr1:36633790-36633809 None:intergenic 25.0%
! TGATGCATGATAAAACATTA+TGG + Chr1:36633333-36633352 MsG0180002317.01.T01:intron 25.0%
!! CATGTTTTCATGTAAACAAA+TGG + Chr1:36633435-36633454 MsG0180002317.01.T01:intron 25.0%
!! TGATTTAAGTTTACGTAGAA+GGG - Chr1:36631623-36631642 None:intergenic 25.0%
!! TTAGATGAAGAAGACAATTT+TGG + Chr1:36631919-36631938 MsG0180002317.01.T01:intron 25.0%
!! TTGATTTAAGTTTACGTAGA+AGG - Chr1:36631624-36631643 None:intergenic 25.0%
!!! ATTCAATTGACGATTTTAGT+AGG + Chr1:36631813-36631832 MsG0180002317.01.T01:intron 25.0%
!!! GAATACGTAATAGTTTTTTG+TGG - Chr1:36631295-36631314 None:intergenic 25.0%
AAGACCCAAATGAAATGTTA+AGG + Chr1:36633782-36633801 MsG0180002317.01.T01:three_prime_UTR 30.0%
ACAACAACTATAAACATGCA+TGG + Chr1:36631677-36631696 MsG0180002317.01.T01:intron 30.0%
AGGTGTAACTAGGATTATAT+GGG + Chr1:36631968-36631987 MsG0180002317.01.T01:intron 30.0%
ATTAATCGAGTACGTATCAA+TGG + Chr1:36631779-36631798 MsG0180002317.01.T01:intron 30.0%
CAGTAAAAACATAGTAACTC+CGG + Chr1:36632053-36632072 MsG0180002317.01.T01:intron 30.0%
GACAATGCGTAATATATGTA+AGG + Chr1:36630840-36630859 MsG0180002317.01.T01:intron 30.0%
GTATGTAAACTCAGTTTACA+TGG - Chr1:36631404-36631423 None:intergenic 30.0%
TAGGTGTAACTAGGATTATA+TGG + Chr1:36631967-36631986 MsG0180002317.01.T01:intron 30.0%
TATAATATTAGAGGGAGAGT+TGG + Chr1:36633124-36633143 MsG0180002317.01.T01:intron 30.0%
TCAGAATGATAATAAGCGAA+GGG + Chr1:36631885-36631904 MsG0180002317.01.T01:intron 30.0%
TCATTTGGGTCTTCAATAAT+CGG - Chr1:36633775-36633794 None:intergenic 30.0%
TTACATACACCATGTAAACT+GGG + Chr1:36631517-36631536 MsG0180002317.01.T01:intron 30.0%
TTCAGAATGATAATAAGCGA+AGG + Chr1:36631884-36631903 MsG0180002317.01.T01:intron 30.0%
TTTACATACACCATGTAAAC+TGG + Chr1:36631516-36631535 MsG0180002317.01.T01:intron 30.0%
! ACGTATTTAAGTTTACACGT+AGG - Chr1:36631598-36631617 None:intergenic 30.0%
! CTTTCTCTTATAGCTTTTCT+AGG - Chr1:36633283-36633302 None:intergenic 30.0%
! TATTGATTGCATATACATGG+TGG + Chr1:36631836-36631855 MsG0180002317.01.T01:intron 30.0%
! TTATCTCCTTGCTATATTGA+AGG - Chr1:36633717-36633736 None:intergenic 30.0%
!! AGGTATTGATTGCATATACA+TGG + Chr1:36631833-36631852 MsG0180002317.01.T01:intron 30.0%
!! GTTGTTGTTATGTTTTTCGT+TGG + Chr1:36633406-36633425 MsG0180002317.01.T01:intron 30.0%
!!! ATGCTCTTTTTTTTTTGTGG+TGG - Chr1:36630883-36630902 None:intergenic 30.0%
!!! TCTTTTTTTTTTGTGGTGGT+CGG - Chr1:36630879-36630898 None:intergenic 30.0%
AAACTAAGCATAGGTGGTTT+AGG + Chr1:36633173-36633192 MsG0180002317.01.T01:intron 35.0%
AAGAACACGAAATTCACGAA+TGG - Chr1:36632167-36632186 None:intergenic 35.0%
AAGATTACTCTACTGATGAC+CGG - Chr1:36632075-36632094 None:intergenic 35.0%
AAGGTTATCGAAGTTAAGCT+CGG + Chr1:36632435-36632454 MsG0180002317.01.T01:CDS 35.0%
CAATCTAACTGATACGTAGA+AGG - Chr1:36632576-36632595 None:intergenic 35.0%
CTTCTACGTATCAGTTAGAT+TGG + Chr1:36632574-36632593 MsG0180002317.01.T01:exon 35.0%
GAAATTCTTACATTGAGACG+TGG + Chr1:36632628-36632647 MsG0180002317.01.T01:intron 35.0%
GGGTGTAACTTGGATTATTT+GGG - Chr1:36631219-36631238 None:intergenic 35.0%
GGTGTAACTAGGATTATATG+GGG + Chr1:36631969-36631988 MsG0180002317.01.T01:intron 35.0%
GGTGTAACTTGGATTATTTG+GGG - Chr1:36631218-36631237 None:intergenic 35.0%
TCACGAATGGCGAATATATT+CGG - Chr1:36632154-36632173 None:intergenic 35.0%
! AATGTGTTGTTGGATTTGCT+TGG + Chr1:36632954-36632973 MsG0180002317.01.T01:intron 35.0%
! CTAACCAAATTTGTTTACGC+GGG - Chr1:36632116-36632135 None:intergenic 35.0%
! GCTAACCAAATTTGTTTACG+CGG - Chr1:36632117-36632136 None:intergenic 35.0%
! TGATCTTAGATCAGTTGGTA+GGG + Chr1:36630818-36630837 MsG0180002317.01.T01:five_prime_UTR 35.0%
!!! CTTTTTTTTTTGTGGTGGTC+GGG - Chr1:36630878-36630897 None:intergenic 35.0%
!!! TTTTTTTTTTGTGGTGGTCG+GGG - Chr1:36630877-36630896 None:intergenic 35.0%
AACTATGTGTGTCTCAGTGT+CGG + Chr1:36632864-36632883 MsG0180002317.01.T01:intron 40.0%
AATGGCGAATATATTCGGCT+TGG - Chr1:36632149-36632168 None:intergenic 40.0%
ACACCATGTAAACTGGGTTT+AGG + Chr1:36631523-36631542 MsG0180002317.01.T01:intron 40.0%
ACACCTAAACCCAGTTTACA+TGG - Chr1:36631529-36631548 None:intergenic 40.0%
ACATAGAAGAAATCGCACTC+CGG - Chr1:36632545-36632564 None:intergenic 40.0%
AGATAACATCGCTTCTCGTA+TGG + Chr1:36633731-36633750 MsG0180002317.01.T01:three_prime_UTR 40.0%
AGTAAGGAGAGTAGATCAGA+TGG + Chr1:36633225-36633244 MsG0180002317.01.T01:intron 40.0%
CAATCAGCATCATCAATCTC+CGG - Chr1:36632471-36632490 None:intergenic 40.0%
GATTGCGAGAGAATTGTTGT+TGG - Chr1:36632289-36632308 None:intergenic 40.0%
GATTGGCGAAGAATTGCATA+TGG + Chr1:36632486-36632505 MsG0180002317.01.T01:CDS 40.0%
GCATGTCCTTCAATATAGCA+AGG + Chr1:36633708-36633727 MsG0180002317.01.T01:three_prime_UTR 40.0%
GGGGTGTAACTTGGATTATT+TGG - Chr1:36631220-36631239 None:intergenic 40.0%
GGTCTTCAATAATCGGACTA+GGG - Chr1:36633768-36633787 None:intergenic 40.0%
GTGATCTTAGATCAGTTGGT+AGG + Chr1:36630817-36630836 MsG0180002317.01.T01:five_prime_UTR 40.0%
TGCATATACATGGTGGTTTG+TGG + Chr1:36631843-36631862 MsG0180002317.01.T01:intron 40.0%
TTAGCCTAACCTCATTGCAA+GGG + Chr1:36633531-36633550 MsG0180002317.01.T01:intron 40.0%
TTTAGCCTAACCTCATTGCA+AGG + Chr1:36633530-36633549 MsG0180002317.01.T01:intron 40.0%
! AACTTGGATTATTTGGGGTG+TGG - Chr1:36631213-36631232 None:intergenic 40.0%
! ACTTGGATTATTTGGGGTGT+GGG - Chr1:36631212-36631231 None:intergenic 40.0%
! GAAAGCACAGTGATTTCAAG+AGG - Chr1:36633560-36633579 None:intergenic 40.0%
! GCATATACATGGTGGTTTGT+GGG + Chr1:36631844-36631863 MsG0180002317.01.T01:intron 40.0%
!! AGTAACTTTGGGGTGTAACT+TGG - Chr1:36631229-36631248 None:intergenic 40.0%
!! CGAATATATTCGGCTTGGTT+TGG - Chr1:36632144-36632163 None:intergenic 40.0%
!! GACTTGCTGATTCTGTAGTA+AGG + Chr1:36633209-36633228 MsG0180002317.01.T01:intron 40.0%
!! TTTGGGTTGACAATCATGTG+CGG + Chr1:36633628-36633647 MsG0180002317.01.T01:intron 40.0%
AAGGAGAGTAGATCAGATGG+AGG + Chr1:36633228-36633247 MsG0180002317.01.T01:intron 45.0%
AGCAGGACATGTAGTAAGGA+TGG + Chr1:36633147-36633166 MsG0180002317.01.T01:intron 45.0%
AGGACATGTAGTAAGGATGG+TGG + Chr1:36633150-36633169 MsG0180002317.01.T01:intron 45.0%
AGGAGAGTAGATCAGATGGA+GGG + Chr1:36633229-36633248 MsG0180002317.01.T01:intron 45.0%
CCTCGTGATCTTAGATCAGT+TGG + Chr1:36630813-36630832 MsG0180002317.01.T01:five_prime_UTR 45.0%
CCTTCCCGCGTAAACAAATT+TGG + Chr1:36632109-36632128 MsG0180002317.01.T01:intron 45.0%
CTTCTCGTATGGCCAAAGTT+GGG + Chr1:36633742-36633761 MsG0180002317.01.T01:three_prime_UTR 45.0%
GGGTCTTCAATAATCGGACT+AGG - Chr1:36633769-36633788 None:intergenic 45.0%
GTATCCCTTGCAATGAGGTT+AGG - Chr1:36633538-36633557 None:intergenic 45.0%
GTGTGTGCACTGTTGACAAT+AGG + Chr1:36633657-36633676 MsG0180002317.01.T01:intron 45.0%
GTTATCGAAGTTAAGCTCGG+AGG + Chr1:36632438-36632457 MsG0180002317.01.T01:CDS 45.0%
TGAAGTTCTGTCCTGATCTC+TGG + Chr1:36633475-36633494 MsG0180002317.01.T01:intron 45.0%
TGGTGGAAACTAAGCATAGG+TGG + Chr1:36633167-36633186 MsG0180002317.01.T01:intron 45.0%
TTACATTGAGACGTGGACAC+CGG + Chr1:36632635-36632654 MsG0180002317.01.T01:intron 45.0%
TTGAGAGGGTTCTGCGAAAA+GGG - Chr1:36632391-36632410 None:intergenic 45.0%
TTGGAGCAGGACATGTAGTA+AGG + Chr1:36633143-36633162 MsG0180002317.01.T01:intron 45.0%
TTTGAGAGGGTTCTGCGAAA+AGG - Chr1:36632392-36632411 None:intergenic 45.0%
! AGTGCCTTCAATCTGAAGTC+TGG + Chr1:36632895-36632914 MsG0180002317.01.T01:intron 45.0%
! CCAAATTTGTTTACGCGGGA+AGG - Chr1:36632112-36632131 None:intergenic 45.0%
! CCAACTGATCTAAGATCACG+AGG - Chr1:36630816-36630835 None:intergenic 45.0%
! GGTGGTTTGTGGGTGAAAAA+GGG + Chr1:36631854-36631873 MsG0180002317.01.T01:intron 45.0%
! GTGCCTTCAATCTGAAGTCT+GGG + Chr1:36632896-36632915 MsG0180002317.01.T01:intron 45.0%
! GTGCCTTCAATCTGAAGTGT+CGG + Chr1:36632707-36632726 MsG0180002317.01.T01:intron 45.0%
! GTGGTTTGTGGGTGAAAAAG+GGG + Chr1:36631855-36631874 MsG0180002317.01.T01:intron 45.0%
! TGGTGGTTTGTGGGTGAAAA+AGG + Chr1:36631853-36631872 MsG0180002317.01.T01:intron 45.0%
!! AAGAGGTATCCCTTGCAATG+AGG - Chr1:36633543-36633562 None:intergenic 45.0%
!! AGAATTGTTGTTGGTTCGCG+CGG - Chr1:36632280-36632299 None:intergenic 45.0%
!! ATATTCGGCTTGGTTTGGCT+CGG - Chr1:36632139-36632158 None:intergenic 45.0%
!! CGGAGATTGATGATGCTGAT+TGG + Chr1:36632469-36632488 MsG0180002317.01.T01:CDS 45.0%
!! GGATGGTGGAAACTAAGCAT+AGG + Chr1:36633164-36633183 MsG0180002317.01.T01:intron 45.0%
!!! TGAATTTTTATTTTAATTAA+AGG - Chr1:36631258-36631277 None:intergenic 5.0%
!!! TGTAAATTTTAATAAAATTT+AGG + Chr1:36631948-36631967 MsG0180002317.01.T01:intron 5.0%
ACTAAGTGGGGTAGGCTACA+TGG - Chr1:36633370-36633389 None:intergenic 50.0%
ATCGAAGTTAAGCTCGGAGG+TGG + Chr1:36632441-36632460 MsG0180002317.01.T01:CDS 50.0%
ATGTAGCCTACCCCACTTAG+TGG + Chr1:36633369-36633388 MsG0180002317.01.T01:intron 50.0%
ATTAGAGGGAGAGTTGGAGC+AGG + Chr1:36633130-36633149 MsG0180002317.01.T01:intron 50.0%
CCACTTAGTGGGATAAGGCT+CGG + Chr1:36633381-36633400 MsG0180002317.01.T01:intron 50.0%
CTACCCCACTTAGTGGGATA+AGG + Chr1:36633376-36633395 MsG0180002317.01.T01:intron 50.0%
CTTATCCCACTAAGTGGGGT+AGG - Chr1:36633378-36633397 None:intergenic 50.0%
GCACCCAGACTTCAGATTGA+AGG - Chr1:36632902-36632921 None:intergenic 50.0%
GCTTCTCGTATGGCCAAAGT+TGG + Chr1:36633741-36633760 MsG0180002317.01.T01:three_prime_UTR 50.0%
GGATTAGGTTCCGTTGTCGA+CGG - Chr1:36632251-36632270 None:intergenic 50.0%
GTTTACGCGGGAAGGAGTTA+TGG - Chr1:36632104-36632123 None:intergenic 50.0%
TGTAGCCTACCCCACTTAGT+GGG + Chr1:36633370-36633389 MsG0180002317.01.T01:intron 50.0%
TGTGGGTGAAAAAGGGGTGA+GGG + Chr1:36631861-36631880 MsG0180002317.01.T01:intron 50.0%
TTGTGGGTGAAAAAGGGGTG+AGG + Chr1:36631860-36631879 MsG0180002317.01.T01:intron 50.0%
! CATTTTGTGGAGTGGGAGTG+TGG - Chr1:36632218-36632237 None:intergenic 50.0%
! CGAGCCTTATCCCACTAAGT+GGG - Chr1:36633383-36633402 None:intergenic 50.0%
! GAGCCTTATCCCACTAAGTG+GGG - Chr1:36633382-36633401 None:intergenic 50.0%
! GCACCGACACTTCAGATTGA+AGG - Chr1:36632713-36632732 None:intergenic 50.0%
! GGAGTGTGGTTAGGGTTTTG+GGG - Chr1:36632204-36632223 None:intergenic 50.0%
! TGGGGGACATTTTGTGGAGT+GGG - Chr1:36632225-36632244 None:intergenic 50.0%
! TTGGGGGACATTTTGTGGAG+TGG - Chr1:36632226-36632245 None:intergenic 50.0%
!! CGACTCTGAAGCAACGCAAA+CGG - Chr1:36632522-36632541 None:intergenic 50.0%
!! GAGTGTGGTTAGGGTTTTGG+GGG - Chr1:36632203-36632222 None:intergenic 50.0%
!! GGGAGTGTGGTTAGGGTTTT+GGG - Chr1:36632205-36632224 None:intergenic 50.0%
!! TGGGAGTGTGGTTAGGGTTT+TGG - Chr1:36632206-36632225 None:intergenic 50.0%
!!! AGTGTGGTTAGGGTTTTGGG+GGG - Chr1:36632202-36632221 None:intergenic 50.0%
GATAACCTTAGCGCCGTCTC+CGG - Chr1:36632424-36632443 None:intergenic 55.0%
GTTCCGTTGTCGACGGTGTT+GGG - Chr1:36632244-36632263 None:intergenic 55.0%
GTTCTGCGAAAAGGGAGACG+CGG - Chr1:36632383-36632402 None:intergenic 55.0%
TAAGCTCGGAGGTGGAGATC+CGG + Chr1:36632449-36632468 MsG0180002317.01.T01:CDS 55.0%
TTCGCGCGGAGAATGGGATT+AGG - Chr1:36632266-36632285 None:intergenic 55.0%
TTGCGTTGCTTCAGAGTCGC+CGG + Chr1:36632523-36632542 MsG0180002317.01.T01:CDS 55.0%
! ATCGGACTAGGGCCCAACTT+TGG - Chr1:36633757-36633776 None:intergenic 55.0%
! CCGAGCCTTATCCCACTAAG+TGG - Chr1:36633384-36633403 None:intergenic 55.0%
! CTGAAGTCTGGGTGCTGCAT+AGG + Chr1:36632907-36632926 MsG0180002317.01.T01:intron 55.0%
! GTGGAGTGGGAGTGTGGTTA+GGG - Chr1:36632212-36632231 None:intergenic 55.0%
! TGTGGAGTGGGAGTGTGGTT+AGG - Chr1:36632213-36632232 None:intergenic 55.0%
! TTCCGTTGTCGACGGTGTTG+GGG - Chr1:36632243-36632262 None:intergenic 55.0%
!! CGGTGTTGGGGGACATTTTG+TGG - Chr1:36632231-36632250 None:intergenic 55.0%
!! CTGAAGTGTCGGTGCTGAGT+AGG + Chr1:36632718-36632737 MsG0180002317.01.T01:intron 55.0%
!! TGTTGGTTCGCGCGGAGAAT+GGG - Chr1:36632272-36632291 None:intergenic 55.0%
!! TTGTTGGTTCGCGCGGAGAA+TGG - Chr1:36632273-36632292 None:intergenic 55.0%
AGACTGGACGGGTGAGGTTG+TGG - Chr1:36632323-36632342 None:intergenic 60.0%
CAAACCTCCGATGCCGGAGA+CGG + Chr1:36632408-36632427 MsG0180002317.01.T01:exon 60.0%
CCCTCTCAAACCTCCGATGC+CGG + Chr1:36632402-36632421 MsG0180002317.01.T01:exon 60.0%
GAGATTGCGAGGCGAGAGAC+TGG - Chr1:36632339-36632358 None:intergenic 60.0%
GGTTCCGTTGTCGACGGTGT+TGG - Chr1:36632245-36632264 None:intergenic 60.0%
TCCCCCAACACCGTCGACAA+CGG + Chr1:36632238-36632257 MsG0180002317.01.T01:intron 60.0%
TCTCTCGCCTCGCAATCTCC+TGG + Chr1:36632340-36632359 MsG0180002317.01.T01:intron 60.0%
TGCGAAAAGGGAGACGCGGA+GGG - Chr1:36632379-36632398 None:intergenic 60.0%
TTGCGAGGCGAGAGACTGGA+CGG - Chr1:36632335-36632354 None:intergenic 60.0%
! CCGGCATCGGAGGTTTGAGA+GGG - Chr1:36632405-36632424 None:intergenic 60.0%
! GGGTGAGGTTGTGGTGGAGA+GGG - Chr1:36632314-36632333 None:intergenic 60.0%
! TCCGGCATCGGAGGTTTGAG+AGG - Chr1:36632406-36632425 None:intergenic 60.0%
! TCCGTTGTCGACGGTGTTGG+GGG - Chr1:36632242-36632261 None:intergenic 60.0%
CTCGCAATCTCCTGGTCCCG+CGG + Chr1:36632348-36632367 MsG0180002317.01.T01:intron 65.0%
CTGCGAAAAGGGAGACGCGG+AGG - Chr1:36632380-36632399 None:intergenic 65.0%
CTGGACGGGTGAGGTTGTGG+TGG - Chr1:36632320-36632339 None:intergenic 65.0%
CTTAGCGCCGTCTCCGGCAT+CGG - Chr1:36632418-36632437 None:intergenic 65.0%
TGCGAGGCGAGAGACTGGAC+GGG - Chr1:36632334-36632353 None:intergenic 65.0%
! CGGGTGAGGTTGTGGTGGAG+AGG - Chr1:36632315-36632334 None:intergenic 65.0%
AGACGCGGAGGGAGTTGCCG+CGG - Chr1:36632368-36632387 None:intergenic 70.0%
CGATGCCGGAGACGGCGCTA+AGG + Chr1:36632416-36632435 MsG0180002317.01.T01:exon 70.0%
CGCGGGACCAGGAGATTGCG+AGG - Chr1:36632350-36632369 None:intergenic 70.0%
GGCGAGAGACTGGACGGGTG+AGG - Chr1:36632329-36632348 None:intergenic 70.0%
GACGCGGAGGGAGTTGCCGC+GGG - Chr1:36632367-36632386 None:intergenic 75.0%
GAGGGAGTTGCCGCGGGACC+AGG - Chr1:36632361-36632380 None:intergenic 75.0%
! AGCGCCGTCTCCGGCATCGG+AGG - Chr1:36632415-36632434 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr1 gene 36630805 36633822 36630805 ID=MsG0180002317.01;Name=MsG0180002317.01
Chr1 mRNA 36630805 36633822 36630805 ID=MsG0180002317.01.T01;Parent=MsG0180002317.01;Name=MsG0180002317.01.T01;_AED=0.50;_eAED=1.00;_QI=70|0|0|1|0|0.33|3|125|57
Chr1 exon 36630805 36630856 36630805 ID=MsG0180002317.01.T01:exon:107;Parent=MsG0180002317.01.T01
Chr1 exon 36632400 36632595 36632400 ID=MsG0180002317.01.T01:exon:106;Parent=MsG0180002317.01.T01
Chr1 exon 36633702 36633822 36633702 ID=MsG0180002317.01.T01:exon:105;Parent=MsG0180002317.01.T01
Chr1 five_prime_UTR 36630805 36630856 36630805 ID=MsG0180002317.01.T01:five_prime_utr;Parent=MsG0180002317.01.T01
Chr1 five_prime_UTR 36632400 36632417 36632400 ID=MsG0180002317.01.T01:five_prime_utr;Parent=MsG0180002317.01.T01
Chr1 CDS 36632418 36632591 36632418 ID=MsG0180002317.01.T01:cds;Parent=MsG0180002317.01.T01
Chr1 three_prime_UTR 36632592 36632595 36632592 ID=MsG0180002317.01.T01:three_prime_utr;Parent=MsG0180002317.01.T01
Chr1 three_prime_UTR 36633702 36633822 36633702 ID=MsG0180002317.01.T01:three_prime_utr;Parent=MsG0180002317.01.T01
Gene Sequence

>MsG0180002317.01.T01

ATGCCGGAGACGGCGCTAAGGTTATCGAAGTTAAGCTCGGAGGTGGAGATCCGGAGATTGATGATGCTGATTGGCGAAGAATTGCATATGGATCTGTTGCTCCGTTTGCGTTGCTTCAGAGTCGCCGGAGTGCGATTTCTTCTATGTCGAAGTCTCCTTCTACGTATCAGTTAG
Protein sequence

>MsG0180002317.01.T01

MPETALRLSKLSSEVEIRRLMMLIGEELHMDLLLRLRCFRVAGVRFLLCRSLLLRIS*