Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019380.01.T01 | XP_003590733.2 | 98.333 | 120 | 2 | 0 | 1 | 120 | 1 | 120 | 1.57E-80 | 242 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019380.01.T01 | G7I2P6 | 98.333 | 120 | 2 | 0 | 1 | 120 | 1 | 120 | 7.49e-81 | 242 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047791.01 | MsG0480019380.01 | 0.846487 | 2.106337e-59 | 3.631458e-56 |
| MsG0080048152.01 | MsG0480019380.01 | 0.808905 | 2.461102e-50 | 1.424491e-47 |
| MsG0080048197.01 | MsG0480019380.01 | 0.836298 | 1.015545e-56 | 1.274651e-53 |
| MsG0080048687.01 | MsG0480019380.01 | 0.805697 | 1.177702e-49 | 6.272762e-47 |
| MsG0080048784.01 | MsG0480019380.01 | 0.805294 | 1.430735e-49 | 7.541477e-47 |
| MsG0080048843.01 | MsG0480019380.01 | 0.820278 | 7.463128e-53 | 5.879113e-50 |
| MsG0080048968.01 | MsG0480019380.01 | 0.811796 | 5.853230e-51 | 3.657553e-48 |
| MsG0180000318.01 | MsG0480019380.01 | -0.819866 | 9.272472e-53 | 7.221340e-50 |
| MsG0180000355.01 | MsG0480019380.01 | 0.815668 | 8.214245e-52 | 5.699380e-49 |
| MsG0180000384.01 | MsG0480019380.01 | 0.828736 | 7.618214e-55 | 7.637351e-52 |
| MsG0180000568.01 | MsG0480019380.01 | 0.809930 | 1.482878e-50 | 8.820642e-48 |
| MsG0180000611.01 | MsG0480019380.01 | 0.816273 | 6.020467e-52 | 4.247025e-49 |
| MsG0180000614.01 | MsG0480019380.01 | 0.822608 | 2.162613e-53 | 1.819141e-50 |
| MsG0180002317.01 | MsG0480019380.01 | -0.806040 | 9.977783e-50 | 5.361008e-47 |
| MsG0180002482.01 | MsG0480019380.01 | 0.801558 | 8.509413e-49 | 4.076029e-46 |
| MsG0180002547.01 | MsG0480019380.01 | 0.823761 | 1.164111e-53 | 1.011650e-50 |
| MsG0180003174.01 | MsG0480019380.01 | 0.815323 | 9.806649e-52 | 6.739788e-49 |
| MsG0180003493.01 | MsG0480019380.01 | 0.800845 | 1.190497e-48 | 5.600966e-46 |
| MsG0180003651.01 | MsG0480019380.01 | 0.821608 | 3.688800e-53 | 3.015229e-50 |
| MsG0180003697.01 | MsG0480019380.01 | 0.804261 | 2.352113e-49 | 1.207206e-46 |
| MsG0180003793.01 | MsG0480019380.01 | 0.856528 | 3.027073e-62 | 7.247768e-59 |
| MsG0180003922.01 | MsG0480019380.01 | 0.816470 | 5.438276e-52 | 3.856756e-49 |
| MsG0180004009.01 | MsG0480019380.01 | 0.811561 | 6.581664e-51 | 4.087424e-48 |
| MsG0180004128.01 | MsG0480019380.01 | 0.809134 | 2.198172e-50 | 1.280251e-47 |
| MsG0180004130.01 | MsG0480019380.01 | 0.835341 | 1.775073e-56 | 2.164600e-53 |
| MsG0180004160.01 | MsG0480019380.01 | 0.802563 | 5.288174e-49 | 2.598562e-46 |
| MsG0180004398.01 | MsG0480019380.01 | 0.842392 | 2.659761e-58 | 4.027402e-55 |
| MsG0180004481.01 | MsG0480019380.01 | 0.804186 | 2.437719e-49 | 1.248750e-46 |
| MsG0180004482.01 | MsG0480019380.01 | 0.800928 | 1.145051e-48 | 5.398507e-46 |
| MsG0180004854.01 | MsG0480019380.01 | 0.815690 | 8.124508e-52 | 5.640416e-49 |
| MsG0180004861.01 | MsG0480019380.01 | 0.813923 | 2.001847e-51 | 1.324554e-48 |
| MsG0180005193.01 | MsG0480019380.01 | 0.804847 | 1.774585e-49 | 9.247503e-47 |
| MsG0180005268.01 | MsG0480019380.01 | 0.806900 | 6.570550e-50 | 3.608877e-47 |
| MsG0180005303.01 | MsG0480019380.01 | -0.801977 | 6.980139e-49 | 3.379115e-46 |
| MsG0180005334.01 | MsG0480019380.01 | 0.845435 | 4.069524e-59 | 6.781019e-56 |
| MsG0180005382.01 | MsG0480019380.01 | 0.803220 | 3.867476e-49 | 1.932629e-46 |
| MsG0180005424.01 | MsG0480019380.01 | 0.822053 | 2.910003e-53 | 2.408909e-50 |
| MsG0180005913.01 | MsG0480019380.01 | 0.823907 | 1.075854e-53 | 9.387322e-51 |
| MsG0280010776.01 | MsG0480019380.01 | 0.866673 | 2.400234e-65 | 8.158357e-62 |
| MsG0280010801.01 | MsG0480019380.01 | 0.801350 | 9.387087e-49 | 4.473010e-46 |
| MsG0280011371.01 | MsG0480019380.01 | 0.806223 | 9.132555e-50 | 4.929962e-47 |
| MsG0280011387.01 | MsG0480019380.01 | 0.813846 | 2.081439e-51 | 1.374367e-48 |
| MsG0380011614.01 | MsG0480019380.01 | 0.831528 | 1.586506e-55 | 1.726307e-52 |
| MsG0380011704.01 | MsG0480019380.01 | 0.823531 | 1.317467e-53 | 1.137337e-50 |
| MsG0380011747.01 | MsG0480019380.01 | 0.861761 | 8.174449e-64 | 2.337951e-60 |
| MsG0380012073.01 | MsG0480019380.01 | 0.818909 | 1.532418e-52 | 1.162208e-49 |
| MsG0380012570.01 | MsG0480019380.01 | 0.801232 | 9.920409e-49 | 4.713195e-46 |
| MsG0380012661.01 | MsG0480019380.01 | -0.812846 | 3.451865e-51 | 2.218325e-48 |
| MsG0380013085.01 | MsG0480019380.01 | 0.812225 | 4.718438e-51 | 2.983083e-48 |
| MsG0380014175.01 | MsG0480019380.01 | 0.838244 | 3.226841e-57 | 4.296015e-54 |
| MsG0480018477.01 | MsG0480019380.01 | 0.836388 | 9.636058e-57 | 1.212549e-53 |
| MsG0480018906.01 | MsG0480019380.01 | 0.817538 | 3.130810e-52 | 2.286293e-49 |
| MsG0480018911.01 | MsG0480019380.01 | 0.800677 | 1.287974e-48 | 6.033737e-46 |
| MsG0480019163.01 | MsG0480019380.01 | -0.829645 | 4.584923e-55 | 4.721117e-52 |
| MsG0480019380.01 | MsG0480021006.01 | 0.822754 | 2.000037e-53 | 1.689245e-50 |
| MsG0480019380.01 | MsG0480021037.01 | 0.821591 | 3.723000e-53 | 3.041710e-50 |
| MsG0480019380.01 | MsG0480021077.01 | -0.822907 | 1.842870e-53 | 1.563196e-50 |
| MsG0480019380.01 | MsG0480021490.01 | -0.801534 | 8.607104e-49 | 4.120395e-46 |
| MsG0480019380.01 | MsG0480021706.01 | 0.841659 | 4.155849e-58 | 6.148246e-55 |
| MsG0480019380.01 | MsG0480021768.01 | 0.805672 | 1.192221e-49 | 6.345627e-47 |
| MsG0480019380.01 | MsG0480021776.01 | -0.829094 | 6.237168e-55 | 6.319468e-52 |
| MsG0480019380.01 | MsG0480022035.01 | 0.835756 | 1.394435e-56 | 1.721519e-53 |
| MsG0480019380.01 | MsG0480022223.01 | 0.829565 | 4.793249e-55 | 4.923818e-52 |
| MsG0480019380.01 | MsG0480022493.01 | 0.827359 | 1.634319e-54 | 1.574368e-51 |
| MsG0480019380.01 | MsG0480022780.01 | 0.853672 | 2.047457e-61 | 4.458273e-58 |
| MsG0480019380.01 | MsG0480022781.01 | -0.807779 | 4.277988e-50 | 2.404031e-47 |
| MsG0480019380.01 | MsG0480022960.01 | 0.822730 | 2.025611e-53 | 1.709677e-50 |
| MsG0480019380.01 | MsG0480023221.01 | 0.829103 | 6.207828e-55 | 6.291081e-52 |
| MsG0480019380.01 | MsG0480023867.01 | 0.801272 | 9.735019e-49 | 4.629775e-46 |
| MsG0480019380.01 | MsG0580024084.01 | 0.851182 | 1.048579e-60 | 2.104915e-57 |
| MsG0480019380.01 | MsG0580024191.01 | 0.844107 | 9.276242e-59 | 1.482242e-55 |
| MsG0480019380.01 | MsG0580024619.01 | 0.811235 | 7.747794e-51 | 4.770337e-48 |
| MsG0480019380.01 | MsG0580025268.01 | 0.852248 | 5.228910e-61 | 1.085979e-57 |
| MsG0480019380.01 | MsG0580025400.01 | 0.833264 | 5.892186e-56 | 6.750181e-53 |
| MsG0480019380.01 | MsG0580025756.01 | 0.826111 | 3.245770e-54 | 3.015704e-51 |
| MsG0480019380.01 | MsG0580026301.01 | 0.804272 | 2.339666e-49 | 1.201165e-46 |
| MsG0480019380.01 | MsG0580027361.01 | 0.803212 | 3.881948e-49 | 1.939475e-46 |
| MsG0480019380.01 | MsG0580028044.01 | 0.837675 | 4.519692e-57 | 5.914656e-54 |
| MsG0480019380.01 | MsG0580028094.01 | 0.801749 | 7.773229e-49 | 3.741416e-46 |
| MsG0480019380.01 | MsG0580028582.01 | 0.810472 | 1.133285e-50 | 6.837599e-48 |
| MsG0480019380.01 | MsG0580028597.01 | 0.845357 | 4.272640e-59 | 7.101906e-56 |
| MsG0480019380.01 | MsG0580029717.01 | 0.832498 | 9.134277e-56 | 1.022799e-52 |
| MsG0480019380.01 | MsG0680030350.01 | 0.856655 | 2.777376e-62 | 6.676391e-59 |
| MsG0480019380.01 | MsG0680030555.01 | 0.801813 | 7.541270e-49 | 3.635850e-46 |
| MsG0480019380.01 | MsG0680031628.01 | 0.809967 | 1.456343e-50 | 8.671118e-48 |
| MsG0480019380.01 | MsG0680031967.01 | 0.851439 | 8.866625e-61 | 1.794814e-57 |
| MsG0480019380.01 | MsG0680032510.01 | -0.805120 | 1.555783e-49 | 8.164240e-47 |
| MsG0480019380.01 | MsG0680032762.01 | 0.818326 | 2.077965e-52 | 1.550635e-49 |
| MsG0480019380.01 | MsG0680035765.01 | 0.816059 | 6.722474e-52 | 4.714456e-49 |
| MsG0480019380.01 | MsG0680035877.01 | 0.823898 | 1.080987e-53 | 9.429750e-51 |
| MsG0480019380.01 | MsG0680035888.01 | 0.821936 | 3.097855e-53 | 2.555927e-50 |
| MsG0480019380.01 | MsG0780036186.01 | 0.850760 | 1.378515e-60 | 2.728953e-57 |
| MsG0480019380.01 | MsG0780036240.01 | 0.833597 | 4.867392e-56 | 5.632021e-53 |
| MsG0480019380.01 | MsG0780036964.01 | 0.828475 | 8.805653e-55 | 8.762058e-52 |
| MsG0480019380.01 | MsG0780037093.01 | 0.817677 | 2.913387e-52 | 2.135605e-49 |
| MsG0480019380.01 | MsG0780038309.01 | -0.805335 | 1.403187e-49 | 7.403833e-47 |
| MsG0480019380.01 | MsG0780038400.01 | 0.861352 | 1.089943e-63 | 3.075267e-60 |
| MsG0480019380.01 | MsG0780038892.01 | 0.847858 | 8.862981e-60 | 1.596537e-56 |
| MsG0480019380.01 | MsG0780039258.01 | 0.802584 | 5.233575e-49 | 2.573221e-46 |
| MsG0480019380.01 | MsG0780039340.01 | 0.816680 | 4.880066e-52 | 3.480848e-49 |
| MsG0480019380.01 | MsG0780039848.01 | 0.834176 | 3.486279e-56 | 4.104320e-53 |
| MsG0480019380.01 | MsG0780039943.01 | 0.802843 | 4.627493e-49 | 2.290450e-46 |
| MsG0480019380.01 | MsG0780040070.01 | 0.833306 | 5.752652e-56 | 6.599044e-53 |
| MsG0480019380.01 | MsG0780040569.01 | 0.852052 | 5.944824e-61 | 1.227048e-57 |
| MsG0480019380.01 | MsG0780040718.01 | 0.821729 | 3.458314e-53 | 2.836652e-50 |
| MsG0480019380.01 | MsG0780041137.01 | 0.813832 | 2.096120e-51 | 1.383544e-48 |
| MsG0480019380.01 | MsG0780041223.01 | 0.874804 | 5.070940e-68 | 2.314320e-64 |
| MsG0480019380.01 | MsG0780041268.01 | 0.809662 | 1.693666e-50 | 1.000394e-47 |
| MsG0480019380.01 | MsG0780041435.01 | 0.817728 | 2.837184e-52 | 2.082750e-49 |
| MsG0480019380.01 | MsG0880042033.01 | 0.846412 | 2.207668e-59 | 3.796955e-56 |
| MsG0480019380.01 | MsG0880042128.01 | 0.822977 | 1.774425e-53 | 1.508142e-50 |
| MsG0480019380.01 | MsG0880042129.01 | 0.819972 | 8.768840e-53 | 6.849611e-50 |
| MsG0480019380.01 | MsG0880042519.01 | 0.820601 | 6.294295e-53 | 5.002396e-50 |
| MsG0480019380.01 | MsG0880042795.01 | 0.845404 | 4.148672e-59 | 6.906098e-56 |
| MsG0480019380.01 | MsG0880042991.01 | 0.817749 | 2.806341e-52 | 2.061307e-49 |
| MsG0480019380.01 | MsG0880043027.01 | -0.823006 | 1.747046e-53 | 1.486116e-50 |
| MsG0480019380.01 | MsG0880045010.01 | -0.805908 | 1.063765e-49 | 5.696278e-47 |
| MsG0480019380.01 | MsG0880045656.01 | 0.808988 | 2.362306e-50 | 1.370382e-47 |
| MsG0480019380.01 | MsG0880045684.01 | 0.848386 | 6.337078e-60 | 1.160962e-56 |
| MsG0480019380.01 | MsG0880046177.01 | 0.818048 | 2.402374e-52 | 1.778949e-49 |
| MsG0480019380.01 | MsG0880046963.01 | -0.825523 | 4.476257e-54 | 4.089747e-51 |
| MsG0480019380.01 | MsG0880047212.01 | 0.859278 | 4.618598e-63 | 1.213063e-59 |
| MsG0480019380.01 | MsG0880047427.01 | 0.824139 | 9.489674e-54 | 8.334313e-51 |
| MsG0480019380.01 | MsG0880047428.01 | 0.805564 | 1.256264e-49 | 6.667596e-47 |
| MsG0480019380.01 | MsG0880047743.01 | 0.800511 | 1.392788e-48 | 6.497101e-46 |
| MsG0380015027.01 | MsG0480019380.01 | 0.867162 | 1.677196e-65 | 5.797520e-62 |
| MsG0380015118.01 | MsG0480019380.01 | 0.842088 | 3.200517e-58 | 4.799752e-55 |
| MsG0380015435.01 | MsG0480019380.01 | 0.819228 | 1.296848e-52 | 9.921138e-50 |
| MsG0380015466.01 | MsG0480019380.01 | 0.813565 | 2.399930e-51 | 1.572684e-48 |
| MsG0380015598.01 | MsG0480019380.01 | -0.841309 | 5.139150e-58 | 7.520202e-55 |
| MsG0380015818.01 | MsG0480019380.01 | 0.827441 | 1.562099e-54 | 1.508285e-51 |
| MsG0380016290.01 | MsG0480019380.01 | 0.826293 | 2.936749e-54 | 2.742783e-51 |
| MsG0380016347.01 | MsG0480019380.01 | -0.812888 | 3.380255e-51 | 2.174821e-48 |
| MsG0380016505.01 | MsG0480019380.01 | 0.812782 | 3.565495e-51 | 2.287504e-48 |
| MsG0380016559.01 | MsG0480019380.01 | 0.809613 | 1.735331e-50 | 1.023708e-47 |
| MsG0380016560.01 | MsG0480019380.01 | 0.829801 | 4.200681e-55 | 4.345840e-52 |
| MsG0380016671.01 | MsG0480019380.01 | 0.814147 | 1.786387e-51 | 1.189236e-48 |
| MsG0380017023.01 | MsG0480019380.01 | 0.812632 | 3.844934e-51 | 2.457273e-48 |
| MsG0380018033.01 | MsG0480019380.01 | 0.837832 | 4.117996e-57 | 5.414904e-54 |
| MsG0480018110.01 | MsG0480019380.01 | 0.805418 | 1.348015e-49 | 7.127989e-47 |
| MsG0280006852.01 | MsG0480019380.01 | 0.808893 | 2.476462e-50 | 1.432885e-47 |
| MsG0280006993.01 | MsG0480019380.01 | 0.819960 | 8.827826e-53 | 6.893231e-50 |
| MsG0280007193.01 | MsG0480019380.01 | -0.803245 | 3.821832e-49 | 1.911045e-46 |
| MsG0280007440.01 | MsG0480019380.01 | 0.826958 | 2.038054e-54 | 1.940218e-51 |
| MsG0280007847.01 | MsG0480019380.01 | 0.860033 | 2.737591e-63 | 7.380736e-60 |
| MsG0280008118.01 | MsG0480019380.01 | 0.825071 | 5.725476e-54 | 5.164599e-51 |
| MsG0280008429.01 | MsG0480019380.01 | 0.820703 | 5.961433e-53 | 4.751674e-50 |
| MsG0280009141.01 | MsG0480019380.01 | 0.825892 | 3.658380e-54 | 3.378117e-51 |
| MsG0280009230.01 | MsG0480019380.01 | 0.800728 | 1.257707e-48 | 5.899407e-46 |
| MsG0280009575.01 | MsG0480019380.01 | 0.827961 | 1.171524e-54 | 1.148427e-51 |
| MsG0280010013.01 | MsG0480019380.01 | 0.835412 | 1.703520e-56 | 2.081607e-53 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019380.01.T01 | MTR_1g073190 | 98.333 | 120 | 2 | 0 | 1 | 120 | 1 | 120 | 1.90e-84 | 242 |
| MsG0480019380.01.T01 | MTR_1g073190 | 98.058 | 103 | 2 | 0 | 1 | 103 | 1 | 103 | 5.42e-71 | 207 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019380.01.T01 | AT1G48200 | 46.610 | 118 | 59 | 3 | 5 | 120 | 3 | 118 | 8.42e-25 | 91.3 |
Find 35 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAGCGACTGCCGCCATTTC+TGG | 0.140183 | 4:+19308604 | MsG0480019380.01.T01:five_prime_UTR |
| CAGATCTCAGATCATTATTT+TGG | 0.170430 | 4:-19309990 | None:intergenic |
| AGTCTATTAGGAACACTCTT+TGG | 0.246535 | 4:+19309033 | MsG0480019380.01.T01:CDS |
| GTTTCGTTATTCAGTGAAAT+TGG | 0.261172 | 4:+19308536 | None:intergenic |
| CCTAATAGACTTATTAGTTT+TGG | 0.270865 | 4:-19309021 | None:intergenic |
| CCAAAACTAATAAGTCTATT+AGG | 0.315405 | 4:+19309021 | MsG0480019380.01.T01:CDS |
| GCAAGGCTCTATGAAATTTA+CGG | 0.323891 | 4:+19309113 | MsG0480019380.01.T01:CDS |
| GTCTATTAGGAACACTCTTT+GGG | 0.365365 | 4:+19309034 | MsG0480019380.01.T01:CDS |
| TAGAGCCTTGCCAAGGGAAC+TGG | 0.374122 | 4:-19309101 | None:intergenic |
| CACAGTGATCAATGTTGGTT+TGG | 0.374270 | 4:+19308914 | MsG0480019380.01.T01:CDS |
| GAAAACTGACTTACCAGAAA+TGG | 0.387735 | 4:-19308617 | None:intergenic |
| ATAATGTAATTGTTTGCATA+AGG | 0.396584 | 4:+19310168 | MsG0480019380.01.T01:three_prime_UTR |
| TTAGGAACACTCTTTGGGAT+TGG | 0.416748 | 4:+19309039 | MsG0480019380.01.T01:CDS |
| CCAGAAATGGCGGCAGTCGC+TGG | 0.418221 | 4:-19308604 | None:intergenic |
| CTGAGATCTGAATTGCTGTC+AGG | 0.427672 | 4:+19310003 | MsG0480019380.01.T01:exon |
| TGCCGAAGCAGCGTCTGATT+CGG | 0.436762 | 4:+19309076 | MsG0480019380.01.T01:CDS |
| AGATGCTAATCCAGCTGGTC+CGG | 0.453182 | 4:+19309967 | MsG0480019380.01.T01:CDS |
| AGCCGAATCAGACGCTGCTT+CGG | 0.457423 | 4:-19309078 | None:intergenic |
| TGATTCGGCTCCAGTTCCCT+TGG | 0.500860 | 4:+19309091 | MsG0480019380.01.T01:CDS |
| CGGCTCCAGTTCCCTTGGCA+AGG | 0.507167 | 4:+19309096 | MsG0480019380.01.T01:CDS |
| GGATTAGCATCTTTCCTCAC+AGG | 0.533126 | 4:-19309956 | None:intergenic |
| TGAAGCTTTAGAAGCTGAGA+AGG | 0.533991 | 4:+19308989 | MsG0480019380.01.T01:CDS |
| TGGCAAGTAGATGTAGAACT+TGG | 0.534881 | 4:+19310116 | MsG0480019380.01.T01:three_prime_UTR |
| CAAGTAGATGTAGAACTTGG+AGG | 0.541262 | 4:+19310119 | MsG0480019380.01.T01:three_prime_UTR |
| TGAGATCTGAATTGCTGTCA+GGG | 0.552769 | 4:+19310004 | MsG0480019380.01.T01:exon |
| ACAGTGATCAATGTTGGTTT+GGG | 0.553382 | 4:+19308915 | MsG0480019380.01.T01:CDS |
| TGTTGCAGAGATAGAGACAA+AGG | 0.559862 | 4:-19308569 | None:intergenic |
| AGTAACACAGTGATCAATGT+TGG | 0.560249 | 4:+19308909 | MsG0480019380.01.T01:CDS |
| CCTCCTCAACAATCTGCACC+TGG | 0.565711 | 4:+19309146 | MsG0480019380.01.T01:CDS |
| AGGAAAGATGCTAATCCAGC+TGG | 0.568309 | 4:+19309962 | MsG0480019380.01.T01:CDS |
| CCAGGTGCAGATTGTTGAGG+AGG | 0.576080 | 4:-19309146 | None:intergenic |
| TTACCAGGTGCAGATTGTTG+AGG | 0.618119 | 4:-19309149 | None:intergenic |
| AATTTCATAGAGCCTTGCCA+AGG | 0.629006 | 4:-19309108 | None:intergenic |
| AACTGACTTACCAGAAATGG+CGG | 0.636737 | 4:-19308614 | None:intergenic |
| ATTTCATAGAGCCTTGCCAA+GGG | 0.656909 | 4:-19309107 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATCATATTATTTTTTCAT+TGG | + | Chr4:19310096-19310115 | MsG0480019380.01.T01:three_prime_UTR | 10.0% |
| !! | ATTTCAAAGTATGAAAAATT+AGG | + | Chr4:19308707-19308726 | MsG0480019380.01.T01:intron | 15.0% |
| !! | GTATAATAAAATATCTAACT+TGG | - | Chr4:19309831-19309850 | None:intergenic | 15.0% |
| !! | TTTCAAAGTATGAAAAATTA+GGG | + | Chr4:19308708-19308727 | MsG0480019380.01.T01:intron | 15.0% |
| !! | AATGATTGTATGCATTTATA+TGG | + | Chr4:19309236-19309255 | MsG0480019380.01.T01:intron | 20.0% |
| !! | AATTAGGGTTATGAAAAATT+AGG | + | Chr4:19308723-19308742 | MsG0480019380.01.T01:intron | 20.0% |
| !! | ATAATGTAATTGTTTGCATA+AGG | + | Chr4:19310168-19310187 | MsG0480019380.01.T01:three_prime_UTR | 20.0% |
| !! | CAATGATAGAAAAAACAAAA+GGG | - | Chr4:19308797-19308816 | None:intergenic | 20.0% |
| !!! | CTTTTGTTTTTTCTATCATT+GGG | + | Chr4:19308796-19308815 | MsG0480019380.01.T01:intron | 20.0% |
| !!! | GATATTTTATTATACCAATC+AGG | + | Chr4:19309836-19309855 | MsG0480019380.01.T01:intron | 20.0% |
| ! | AAAAATTGAATTACATCTGG+AGG | - | Chr4:19309535-19309554 | None:intergenic | 25.0% |
| ! | AAAGAACAAATAATGAGAAG+AGG | - | Chr4:19309593-19309612 | None:intergenic | 25.0% |
| ! | AATTTGATGAATTGTTTGAG+AGG | + | Chr4:19309403-19309422 | MsG0480019380.01.T01:intron | 25.0% |
| ! | AGAGATAAAAACAAATTACC+AGG | - | Chr4:19309167-19309186 | None:intergenic | 25.0% |
| ! | ATTTCAGGAGAGAAAAAAAA+TGG | + | Chr4:19308842-19308861 | MsG0480019380.01.T01:intron | 25.0% |
| ! | ATTTGATGAATTGTTTGAGA+GGG | + | Chr4:19309404-19309423 | MsG0480019380.01.T01:intron | 25.0% |
| ! | CCAAAACTAATAAGTCTATT+AGG | + | Chr4:19309021-19309040 | MsG0480019380.01.T01:CDS | 25.0% |
| ! | CCAATGATAGAAAAAACAAA+AGG | - | Chr4:19308798-19308817 | None:intergenic | 25.0% |
| ! | CCATGTAAAAGAAATTTACA+TGG | - | Chr4:19309768-19309787 | None:intergenic | 25.0% |
| !! | CCAGATGTAATTCAATTTTT+GGG | + | Chr4:19309535-19309554 | MsG0480019380.01.T01:intron | 25.0% |
| !! | CCATGTAAATTTCTTTTACA+TGG | + | Chr4:19309765-19309784 | MsG0480019380.01.T01:intron | 25.0% |
| !! | TCCAGATGTAATTCAATTTT+TGG | + | Chr4:19309534-19309553 | MsG0480019380.01.T01:intron | 25.0% |
| !! | TTTTCTTCAATTCTTTCATC+TGG | + | Chr4:19308666-19308685 | MsG0480019380.01.T01:intron | 25.0% |
| !!! | AGGTTTCTACAATCATTTTT+CGG | + | Chr4:19309316-19309335 | MsG0480019380.01.T01:intron | 25.0% |
| !!! | ATAAAGTATTTTCTGAGAGT+AGG | + | Chr4:19310059-19310078 | MsG0480019380.01.T01:three_prime_UTR | 25.0% |
| !!! | CCTAATAGACTTATTAGTTT+TGG | - | Chr4:19309024-19309043 | None:intergenic | 25.0% |
| !!! | CCTTTTGTTTTTTCTATCAT+TGG | + | Chr4:19308795-19308814 | MsG0480019380.01.T01:intron | 25.0% |
| !!! | TTTTCATACTTTGAAATGAG+CGG | - | Chr4:19308704-19308723 | None:intergenic | 25.0% |
| ACCACAACAGAAGTAATTAT+AGG | - | Chr4:19309923-19309942 | None:intergenic | 30.0% | |
| ACCTATAATTACTTCTGTTG+TGG | + | Chr4:19309919-19309938 | MsG0480019380.01.T01:intron | 30.0% | |
| ATAGGTAAGAAATCATGACA+TGG | - | Chr4:19309905-19309924 | None:intergenic | 30.0% | |
| ATCTTGTAAAGCAGTAGTAA+AGG | + | Chr4:19309790-19309809 | MsG0480019380.01.T01:intron | 30.0% | |
| ATTATACCAATCAGGTGAAT+TGG | + | Chr4:19309844-19309863 | MsG0480019380.01.T01:intron | 30.0% | |
| CAAAACGTGTAACCAAATAT+TGG | + | Chr4:19309559-19309578 | MsG0480019380.01.T01:intron | 30.0% | |
| CATGGTAGTTGTTGTAAATT+TGG | - | Chr4:19309750-19309769 | None:intergenic | 30.0% | |
| CCCAAAAATTGAATTACATC+TGG | - | Chr4:19309538-19309557 | None:intergenic | 30.0% | |
| TAGCATTAACCAATTCAATG+AGG | + | Chr4:19309197-19309216 | MsG0480019380.01.T01:intron | 30.0% | |
| TGTAAGAAACTCATTGATAG+TGG | + | Chr4:19309491-19309510 | MsG0480019380.01.T01:intron | 30.0% | |
| TTCTCTCCTGAAATCAAAAA+GGG | - | Chr4:19308836-19308855 | None:intergenic | 30.0% | |
| TTGTTATACACATAGGTTAC+TGG | + | Chr4:19309628-19309647 | MsG0480019380.01.T01:intron | 30.0% | |
| TTTACTACTGCTTTACAAGA+TGG | - | Chr4:19309791-19309810 | None:intergenic | 30.0% | |
| TTTCTCTCCTGAAATCAAAA+AGG | - | Chr4:19308837-19308856 | None:intergenic | 30.0% | |
| TTTGTTCTTTGCTTTGATTG+TGG | + | Chr4:19309603-19309622 | MsG0480019380.01.T01:intron | 30.0% | |
| ! | CAATATTTGGTTACACGTTT+TGG | - | Chr4:19309561-19309580 | None:intergenic | 30.0% |
| ! | CAGATCTCAGATCATTATTT+TGG | - | Chr4:19309993-19310012 | None:intergenic | 30.0% |
| ! | TTCTATCATTGGGTTGTAAT+GGG | + | Chr4:19308806-19308825 | MsG0480019380.01.T01:intron | 30.0% |
| ! | TTTCTATCATTGGGTTGTAA+TGG | + | Chr4:19308805-19308824 | MsG0480019380.01.T01:intron | 30.0% |
| !! | TGTGGATTTGTTATACACAT+AGG | + | Chr4:19309621-19309640 | MsG0480019380.01.T01:intron | 30.0% |
| !!! | TTCTGGTTAGTTTTGACAAA+GGG | + | Chr4:19309723-19309742 | MsG0480019380.01.T01:intron | 30.0% |
| AACTAACCAGAACAAAGATG+AGG | - | Chr4:19309715-19309734 | None:intergenic | 35.0% | |
| AGATGAGGAGCAAATAACAA+TGG | - | Chr4:19309700-19309719 | None:intergenic | 35.0% | |
| AGTAACACAGTGATCAATGT+TGG | + | Chr4:19308909-19308928 | MsG0480019380.01.T01:CDS | 35.0% | |
| AGTCTATTAGGAACACTCTT+TGG | + | Chr4:19309033-19309052 | MsG0480019380.01.T01:CDS | 35.0% | |
| CAGTAGTAAAGGTTTGATGT+TGG | + | Chr4:19309801-19309820 | MsG0480019380.01.T01:intron | 35.0% | |
| GAGGAACAAACACCAATATT+TGG | - | Chr4:19309574-19309593 | None:intergenic | 35.0% | |
| GCAAGGCTCTATGAAATTTA+CGG | + | Chr4:19309113-19309132 | MsG0480019380.01.T01:CDS | 35.0% | |
| GTCTATTAGGAACACTCTTT+GGG | + | Chr4:19309034-19309053 | MsG0480019380.01.T01:CDS | 35.0% | |
| TAACCAATTCAATGAGGCAA+TGG | + | Chr4:19309203-19309222 | MsG0480019380.01.T01:intron | 35.0% | |
| TCTCTCCTGAAATCAAAAAG+GGG | - | Chr4:19308835-19308854 | None:intergenic | 35.0% | |
| ! | GAAAACTGACTTACCAGAAA+TGG | - | Chr4:19308620-19308639 | None:intergenic | 35.0% |
| ! | GTTGAGTTTGAAGTGATGTT+AGG | - | Chr4:19308645-19308664 | None:intergenic | 35.0% |
| ! | TATAAGCTTTTAGCGTTGCA+TGG | + | Chr4:19310030-19310049 | MsG0480019380.01.T01:three_prime_UTR | 35.0% |
| !! | ACAGTGATCAATGTTGGTTT+GGG | + | Chr4:19308915-19308934 | MsG0480019380.01.T01:CDS | 35.0% |
| !!! | GTTCTGGTTAGTTTTGACAA+AGG | + | Chr4:19309722-19309741 | MsG0480019380.01.T01:intron | 35.0% |
| !!! | TCTCAGATCATTATTTTGGC+CGG | - | Chr4:19309989-19310008 | None:intergenic | 35.0% |
| AATTTCATAGAGCCTTGCCA+AGG | - | Chr4:19309111-19309130 | None:intergenic | 40.0% | |
| ACAACTCGCAGGAAACTATA+TGG | - | Chr4:19309344-19309363 | None:intergenic | 40.0% | |
| ATTCAATGAGGCAATGGAAG+TGG | + | Chr4:19309209-19309228 | MsG0480019380.01.T01:intron | 40.0% | |
| ATTTCATAGAGCCTTGCCAA+GGG | - | Chr4:19309110-19309129 | None:intergenic | 40.0% | |
| CAAGTAGATGTAGAACTTGG+AGG | + | Chr4:19310119-19310138 | MsG0480019380.01.T01:three_prime_UTR | 40.0% | |
| CTTCCATTGCCTCATTGAAT+TGG | - | Chr4:19309209-19309228 | None:intergenic | 40.0% | |
| GATTCACCAATTCACCTGAT+TGG | - | Chr4:19309853-19309872 | None:intergenic | 40.0% | |
| TCCATTGTGCATCGAAAATG+TGG | - | Chr4:19309875-19309894 | None:intergenic | 40.0% | |
| TGAGATCTGAATTGCTGTCA+GGG | + | Chr4:19310004-19310023 | MsG0480019380.01.T01:exon | 40.0% | |
| TGGCAAGTAGATGTAGAACT+TGG | + | Chr4:19310116-19310135 | MsG0480019380.01.T01:three_prime_UTR | 40.0% | |
| TGTTGCAGAGATAGAGACAA+AGG | - | Chr4:19308572-19308591 | None:intergenic | 40.0% | |
| TTTGCTCCTCATCTTTGTTC+TGG | + | Chr4:19309706-19309725 | MsG0480019380.01.T01:intron | 40.0% | |
| ! | AACTGACTTACCAGAAATGG+CGG | - | Chr4:19308617-19308636 | None:intergenic | 40.0% |
| ! | ACTTCTGTTGTGGTTTTTCC+AGG | + | Chr4:19309929-19309948 | MsG0480019380.01.T01:intron | 40.0% |
| ! | CACAGTGATCAATGTTGGTT+TGG | + | Chr4:19308914-19308933 | MsG0480019380.01.T01:CDS | 40.0% |
| ! | TCATTGCTAGCTTGCATGAT+AGG | + | Chr4:19309296-19309315 | MsG0480019380.01.T01:intron | 40.0% |
| ! | TCCACATTTTCGATGCACAA+TGG | + | Chr4:19309871-19309890 | MsG0480019380.01.T01:intron | 40.0% |
| !! | TGAAGCTTTAGAAGCTGAGA+AGG | + | Chr4:19308989-19309008 | MsG0480019380.01.T01:CDS | 40.0% |
| !! | TTAGGAACACTCTTTGGGAT+TGG | + | Chr4:19309039-19309058 | MsG0480019380.01.T01:CDS | 40.0% |
| !!! | GGTGCTGTTTTTGCAATTCT+GGG | + | Chr4:19308936-19308955 | MsG0480019380.01.T01:CDS | 40.0% |
| AGGAAAGATGCTAATCCAGC+TGG | + | Chr4:19309962-19309981 | MsG0480019380.01.T01:CDS | 45.0% | |
| ATTACATCTGGAGGAGAGAG+CGG | - | Chr4:19309526-19309545 | None:intergenic | 45.0% | |
| CTGAGATCTGAATTGCTGTC+AGG | + | Chr4:19310003-19310022 | MsG0480019380.01.T01:exon | 45.0% | |
| TTACCAGGTGCAGATTGTTG+AGG | - | Chr4:19309152-19309171 | None:intergenic | 45.0% | |
| ! | AACACAAAAGCACAACTCGC+AGG | - | Chr4:19309355-19309374 | None:intergenic | 45.0% |
| ! | GGATTAGCATCTTTCCTCAC+AGG | - | Chr4:19309959-19309978 | None:intergenic | 45.0% |
| ! | TTTTTCCAGGAGATCCTGTG+AGG | + | Chr4:19309942-19309961 | MsG0480019380.01.T01:intron | 45.0% |
| !!! | GGGTGCTGTTTTTGCAATTC+TGG | + | Chr4:19308935-19308954 | MsG0480019380.01.T01:CDS | 45.0% |
| !!! | GGTCACCCCTTTTTGATTTC+AGG | + | Chr4:19308827-19308846 | MsG0480019380.01.T01:intron | 45.0% |
| AGATGCTAATCCAGCTGGTC+CGG | + | Chr4:19309967-19309986 | MsG0480019380.01.T01:CDS | 50.0% | |
| TCTTTCCTCACAGGATCTCC+TGG | - | Chr4:19309950-19309969 | None:intergenic | 50.0% | |
| !!! | ATTATTTTGGCCGGACCAGC+TGG | - | Chr4:19309980-19309999 | None:intergenic | 50.0% |
| AGCCGAATCAGACGCTGCTT+CGG | - | Chr4:19309081-19309100 | None:intergenic | 55.0% | |
| CCAGGTGCAGATTGTTGAGG+AGG | - | Chr4:19309149-19309168 | None:intergenic | 55.0% | |
| CCTCCTCAACAATCTGCACC+TGG | + | Chr4:19309146-19309165 | MsG0480019380.01.T01:CDS | 55.0% | |
| TAGAGCCTTGCCAAGGGAAC+TGG | - | Chr4:19309104-19309123 | None:intergenic | 55.0% | |
| TGCCGAAGCAGCGTCTGATT+CGG | + | Chr4:19309076-19309095 | MsG0480019380.01.T01:CDS | 55.0% | |
| ! | TGATTCGGCTCCAGTTCCCT+TGG | + | Chr4:19309091-19309110 | MsG0480019380.01.T01:CDS | 55.0% |
| CCAGAAATGGCGGCAGTCGC+TGG | - | Chr4:19308607-19308626 | None:intergenic | 65.0% | |
| CCAGCGACTGCCGCCATTTC+TGG | + | Chr4:19308604-19308623 | MsG0480019380.01.T01:five_prime_UTR | 65.0% | |
| CGGCTCCAGTTCCCTTGGCA+AGG | + | Chr4:19309096-19309115 | MsG0480019380.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 19308541 | 19310254 | 19308541 | ID=MsG0480019380.01;Name=MsG0480019380.01 |
| Chr4 | mRNA | 19308541 | 19310254 | 19308541 | ID=MsG0480019380.01.T01;Parent=MsG0480019380.01;Name=MsG0480019380.01.T01;_AED=0.47;_eAED=0.47;_QI=97|1|1|1|0.5|0.66|3|248|120 |
| Chr4 | exon | 19308541 | 19308625 | 19308541 | ID=MsG0480019380.01.T01:exon:9415;Parent=MsG0480019380.01.T01 |
| Chr4 | exon | 19308849 | 19309167 | 19308849 | ID=MsG0480019380.01.T01:exon:9416;Parent=MsG0480019380.01.T01 |
| Chr4 | exon | 19309951 | 19310254 | 19309951 | ID=MsG0480019380.01.T01:exon:9417;Parent=MsG0480019380.01.T01 |
| Chr4 | five_prime_UTR | 19308541 | 19308625 | 19308541 | ID=MsG0480019380.01.T01:five_prime_utr;Parent=MsG0480019380.01.T01 |
| Chr4 | five_prime_UTR | 19308849 | 19308860 | 19308849 | ID=MsG0480019380.01.T01:five_prime_utr;Parent=MsG0480019380.01.T01 |
| Chr4 | CDS | 19308861 | 19309167 | 19308861 | ID=MsG0480019380.01.T01:cds;Parent=MsG0480019380.01.T01 |
| Chr4 | CDS | 19309951 | 19310006 | 19309951 | ID=MsG0480019380.01.T01:cds;Parent=MsG0480019380.01.T01 |
| Chr4 | three_prime_UTR | 19310007 | 19310254 | 19310007 | ID=MsG0480019380.01.T01:three_prime_utr;Parent=MsG0480019380.01.T01 |
| Gene Sequence |
| Protein sequence |
