Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029879.01.T01 | KAE9458337.1 | 38.323 | 167 | 57 | 5 | 1 | 167 | 1 | 121 | 1.12E-20 | 92.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029879.01.T01 | A0A6A4LV76 | 38.323 | 167 | 57 | 5 | 1 | 167 | 1 | 121 | 5.33e-21 | 92.4 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048016.01 | MsG0580029879.01 | 0.840393 | 8.935086e-58 | 1.271004e-54 |
| MsG0080048065.01 | MsG0580029879.01 | 0.846306 | 2.360346e-59 | 4.044890e-56 |
| MsG0080048152.01 | MsG0580029879.01 | 0.815484 | 9.028869e-52 | 6.233280e-49 |
| MsG0080048463.01 | MsG0580029879.01 | 0.843509 | 1.341572e-58 | 2.103709e-55 |
| MsG0080048464.01 | MsG0580029879.01 | 0.853019 | 3.150975e-61 | 6.712289e-58 |
| MsG0080048683.01 | MsG0580029879.01 | 0.840860 | 6.741587e-58 | 9.729927e-55 |
| MsG0080048748.01 | MsG0580029879.01 | 0.805196 | 1.500164e-49 | 7.887450e-47 |
| MsG0080048769.01 | MsG0580029879.01 | 0.805522 | 1.281687e-49 | 6.795248e-47 |
| MsG0080048802.01 | MsG0580029879.01 | 0.815744 | 7.902569e-52 | 5.494701e-49 |
| MsG0080048826.01 | MsG0580029879.01 | 0.829346 | 5.419038e-55 | 5.532013e-52 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029879.01.T01 | MTR_5g094130 | 97.059 | 34 | 1 | 0 | 1 | 34 | 1 | 34 | 1.21e-15 | 68.2 |
Find 32 sgRNAs with CRISPR-Local
Find 153 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGTTATGCGCGACAAAAC+AGG | 0.427009 | 5:-103308418 | None:intergenic |
| AAGTTGTTGTTTATTCCCAT+AGG | 0.534082 | 5:+103306503 | MsG0580029879.01.T01:CDS |
| ACAAGAATTTGCTTCAACAA+AGG | 0.488569 | 5:+103306085 | MsG0580029879.01.T01:CDS |
| ACACCAAGGTAAGCTTAATG+AGG | 0.571144 | 5:-103308455 | None:intergenic |
| AGTTGTTGTTTATTCCCATA+GGG | 0.521107 | 5:+103306504 | MsG0580029879.01.T01:CDS |
| ATAAAACAAATCAAACACCA+AGG | 0.719652 | 5:-103308469 | None:intergenic |
| ATAGTTCCCATCTTGAGTGA+TGG | 0.596385 | 5:+103308532 | MsG0580029879.01.T01:CDS |
| ATGAACAGACCCCTTAGTTT+TGG | 0.330361 | 5:-103306338 | None:intergenic |
| ATGATAAGTATGCTAAAATT+TGG | 0.185786 | 5:+103306476 | MsG0580029879.01.T01:intron |
| ATGCGATGATTCCGATGAGT+AGG | 0.593429 | 5:-103306054 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACAAAAGTTTATCGACACA+AGG | - | Chr5:103308326-103308345 | None:intergenic | 30.0% | |
| AAGTGAATAGATTAAGCTTG+GGG | - | Chr5:103307356-103307375 | None:intergenic | 30.0% | |
| AATCTTGTCCAGTATAATCA+AGG | + | Chr5:103308169-103308188 | MsG0580029879.01.T01:intron | 30.0% | |
| AGAAGTGAATAGATTAAGCT+TGG | - | Chr5:103307358-103307377 | None:intergenic | 30.0% | |
| AGTAGACAAAAGAATATGTC+AGG | + | Chr5:103307582-103307601 | MsG0580029879.01.T01:intron | 30.0% | |
| ATGATACAAACACATTGCTT+TGG | + | Chr5:103308219-103308238 | MsG0580029879.01.T01:intron | 30.0% | |
| GAAGTGAATAGATTAAGCTT+GGG | - | Chr5:103307357-103307376 | None:intergenic | 30.0% | |
| GATTATACTGGACAAGATTA+TGG | - | Chr5:103308168-103308187 | None:intergenic | 30.0% | |
| GCACAAATAAACAGTGAAAA+TGG | + | Chr5:103307502-103307521 | MsG0580029879.01.T01:intron | 30.0% | |
| TGAAGCAAATTCTTGTAGAA+AGG | - | Chr5:103306081-103306100 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 103306025 | 103308561 | 103306025 | ID=MsG0580029879.01;Name=MsG0580029879.01 |
| Chr5 | mRNA | 103306025 | 103308561 | 103306025 | ID=MsG0580029879.01.T01;Parent=MsG0580029879.01;Name=MsG0580029879.01.T01;_AED=0.45;_eAED=0.75;_QI=0|0|0.2|1|0|0|5|0|167 |
| Chr5 | exon | 103306025 | 103306146 | 103306025 | ID=MsG0580029879.01.T01:exon:35192;Parent=MsG0580029879.01.T01 |
| Chr5 | exon | 103306336 | 103306413 | 103306336 | ID=MsG0580029879.01.T01:exon:35193;Parent=MsG0580029879.01.T01 |
| Chr5 | exon | 103306484 | 103306541 | 103306484 | ID=MsG0580029879.01.T01:exon:35194;Parent=MsG0580029879.01.T01 |
| Chr5 | exon | 103306968 | 103307015 | 103306968 | ID=MsG0580029879.01.T01:exon:35195;Parent=MsG0580029879.01.T01 |
| Chr5 | exon | 103308364 | 103308561 | 103308364 | ID=MsG0580029879.01.T01:exon:35196;Parent=MsG0580029879.01.T01 |
| Chr5 | CDS | 103306025 | 103306146 | 103306025 | ID=MsG0580029879.01.T01:cds;Parent=MsG0580029879.01.T01 |
| Chr5 | CDS | 103306336 | 103306413 | 103306336 | ID=MsG0580029879.01.T01:cds;Parent=MsG0580029879.01.T01 |
| Chr5 | CDS | 103306484 | 103306541 | 103306484 | ID=MsG0580029879.01.T01:cds;Parent=MsG0580029879.01.T01 |
| Chr5 | CDS | 103306968 | 103307015 | 103306968 | ID=MsG0580029879.01.T01:cds;Parent=MsG0580029879.01.T01 |
| Chr5 | CDS | 103308364 | 103308561 | 103308364 | ID=MsG0580029879.01.T01:cds;Parent=MsG0580029879.01.T01 |
| Gene Sequence |
>MsG0580029879.01.T01 ATGGGTTTCGTTGTTACCTCGCTAATCTTCCTACTCATCGGAATCATCGCATGCCTTTCTACAAGAATTTGCTTCAACAAAGGACCTTCTGCTAATCTGTATGCGCCAAACTTCAATATATTTGCCAAAACTAAGGGGTCTGTTCATAATCTGTTTTCGGCTTATTTTCATAAGCTCTCGAGAATGTCTAATGAAAACAGTATGCTAAAATTTGGAAATAAGTTGTTGTTTATTCCCATAGGGTCTAAGATAAAAAGGATTTCACCTAACTTTGGTTCTTACATCAACAATATGCTGCTGGATGATATGGTACATTTCCTTTTGGTCCCGGTACTAGTTCTAGCTTGTGAACAGTCATGTCCTGTTTTGTCGCGCATAACCTTACTCTTTTCTTCTCCCTCATTAAGCTTACCTTGGTGTTTGATTTGTTTTATCAGGTGGGCGATTGTATATCTGGCACAGATGAACCCACTCATAGTTCCCATCTTGAGTGATGGCGAATAA |
| Protein sequence |
>MsG0580029879.01.T01 MGFVVTSLIFLLIGIIACLSTRICFNKGPSANLYAPNFNIFAKTKGSVHNLFSAYFHKLSRMSNENSMLKFGNKLLFIPIGSKIKRISPNFGSYINNMLLDDMVHFLLVPVLVLACEQSCPVLSRITLLFSSPSLSLPWCLICFIRWAIVYLAQMNPLIVPILSDGE* |
