AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003805.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003805.01.T01 MTR_1g069715 99.462 186 1 0 9 194 492 677 6.05e-127 372
MsG0180003805.01.T01 MTR_7g097170 81.720 186 34 0 9 194 27 212 1.73e-97 281
MsG0180003805.01.T01 MTR_2g047875 72.165 194 50 1 3 192 21 214 2.08e-95 276
MsG0180003805.01.T01 MTR_8g019700 47.682 151 79 0 10 160 29 179 2.48e-33 119
MsG0180003805.01.T01 MTR_2g041190 46.358 151 81 0 10 160 29 179 3.09e-33 118
MsG0180003805.01.T01 MTR_4g057565 30.435 161 112 0 3 163 25 185 6.66e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003805.01.T01 AT1G03950 78.804 184 39 0 9 192 27 210 2.98e-104 298
MsG0180003805.01.T01 AT5G44560 75.773 194 37 1 9 192 7 200 3.20e-102 293
MsG0180003805.01.T01 AT5G44560 75.773 194 37 1 9 192 27 220 5.46e-102 293
MsG0180003805.01.T01 AT5G44560 82.895 152 26 0 9 160 27 178 3.79e-89 259
MsG0180003805.01.T01 AT5G44560 77.419 124 28 0 9 132 27 150 3.01e-65 198
MsG0180003805.01.T01 AT2G06530 42.784 194 100 2 10 192 9 202 3.32e-35 123
MsG0180003805.01.T01 AT2G06530 42.784 194 100 2 10 192 30 223 5.76e-35 123
MsG0180003805.01.T01 AT5G22950 26.829 205 136 2 3 193 25 229 6.21e-11 60.1

Find 37 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGAACTCTTGAATAATTT+TGG 0.113072 1:-68503497 None:intergenic
CCATTTGTGCTGATTGTTTC+TGG 0.121554 1:-68503516 None:intergenic
CTTCTCGATGTCATCAATTT+CGG 0.344181 1:-68505549 None:intergenic
GGCAATGGAAGCTATGAATA+AGG 0.394968 1:+68502938 MsG0180003805.01.T01:CDS
TTTGAGCTCGACTACCTTGA+AGG 0.406647 1:-68502738 None:intergenic
CAACAAATTGCTAACCTTCA+AGG 0.417491 1:+68502724 MsG0180003805.01.T01:CDS
CGGCGTGGATGTTGCTTCAC+AGG 0.421005 1:+68504996 MsG0180003805.01.T01:CDS
CCAGGCAGTTGGTTAGGCTT+AGG 0.424613 1:+68502701 MsG0180003805.01.T01:CDS
TGATGACATCGAGAAGCGTT+TGG 0.440202 1:+68505556 MsG0180003805.01.T01:CDS
CCAGAAACAATCAGCACAAA+TGG 0.451902 1:+68503516 MsG0180003805.01.T01:CDS
CGGCATGAAAGGTGCTAGTA+AGG 0.457818 1:+68502917 MsG0180003805.01.T01:CDS
TACATTACCTCTAGTGGCAT+TGG 0.462651 1:-68502017 None:intergenic
TCTATTTCTGCCGGCATGAA+AGG 0.465107 1:+68502906 MsG0180003805.01.T01:CDS
TGGCAGGTACTCGATGAAAT+CGG 0.471009 1:+68504976 MsG0180003805.01.T01:intron
GACAGAAGATCTGACAAATC+AGG 0.492127 1:+68503692 MsG0180003805.01.T01:CDS
TATTAGCCAGGCAGTTGGTT+AGG 0.498748 1:+68502695 MsG0180003805.01.T01:CDS
GATAAAGAAAACTGCTAAGA+CGG 0.504385 1:+68502308 MsG0180003805.01.T01:CDS
GCACAGTCTTCTATTTCTGC+CGG 0.512916 1:+68502897 MsG0180003805.01.T01:CDS
ATTAATTAGATGTATTCCAC+AGG 0.522510 1:+68502144 MsG0180003805.01.T01:intron
AACTGCTAAGACGGGAAATG+AGG 0.530892 1:+68502317 MsG0180003805.01.T01:CDS
CAGTTATCAGCAGCTCCAAA+AGG 0.534197 1:+68505125 MsG0180003805.01.T01:intron
ATAAAGAAAACTGCTAAGAC+GGG 0.538319 1:+68502309 MsG0180003805.01.T01:CDS
AGTAATTACATTACCTCTAG+TGG 0.549933 1:-68502023 None:intergenic
AGATGCCATCGATGATGTCT+TGG 0.557065 1:+68503647 MsG0180003805.01.T01:CDS
TAAAATATTAGCCAGGCAGT+TGG 0.568904 1:+68502690 MsG0180003805.01.T01:CDS
TGACATCGAGAAGCGTTTGG+CGG 0.572824 1:+68505559 MsG0180003805.01.T01:CDS
AGAATACTGAAAATGTCAGC+AGG 0.574074 1:+68505162 MsG0180003805.01.T01:CDS
CTGCGACTAAAATATTAGCC+AGG 0.584824 1:+68502683 MsG0180003805.01.T01:CDS
GAAAGGTGCTAGTAAGGCAA+TGG 0.588411 1:+68502923 MsG0180003805.01.T01:CDS
CTTACTAGCACCTTTCATGC+CGG 0.602682 1:-68502916 None:intergenic
CCTAAGCCTAACCAACTGCC+TGG 0.602720 1:-68502701 None:intergenic
GGTAGTCGAGCTCAAATGAG+AGG 0.622813 1:+68502745 MsG0180003805.01.T01:CDS
ATTATCCAAGACATCATCGA+TGG 0.636937 1:-68503652 None:intergenic
GAAATGGCCAATGCCACTAG+AGG 0.655907 1:+68502010 None:intergenic
TGATGTCTTGGATAATGATG+AGG 0.669181 1:+68503659 MsG0180003805.01.T01:CDS
AGGCATAGCAACTCACACAC+AGG 0.671175 1:+68502765 MsG0180003805.01.T01:CDS
GGTACTCGATGAAATCGGCG+TGG 0.702596 1:+68504981 MsG0180003805.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTACAAAAAACAATTGA+TGG + Chr1:68504936-68504955 MsG0180003805.01.T01:intron 15.0%
!! CTAATTAATTAATACAGTAA+GGG - Chr1:68502133-68502152 None:intergenic 15.0%
!! TCTAATTAATTAATACAGTA+AGG - Chr1:68502134-68502153 None:intergenic 15.0%
!! TTAAAGCTAAGATTTAAATT+TGG - Chr1:68503937-68503956 None:intergenic 15.0%
!! TTTAATTGGATGTTTATTAA+TGG + Chr1:68503149-68503168 MsG0180003805.01.T01:intron 15.0%
!!! AAATACTTTAAATTTTTGGT+TGG + Chr1:68504800-68504819 MsG0180003805.01.T01:intron 15.0%
!!! TATTAGCAAATTACAATTTT+AGG - Chr1:68504555-68504574 None:intergenic 15.0%
!!! TGGAAATTTTATTTACTTTT+TGG + Chr1:68504956-68504975 MsG0180003805.01.T01:intron 15.0%
!! GAAATCAATAGAAAATTACA+TGG + Chr1:68504033-68504052 MsG0180003805.01.T01:intron 20.0%
!! GTTTGATATCAAAATAGTTA+AGG - Chr1:68504728-68504747 None:intergenic 20.0%
!! TAAAAGGAAATTAATGCATT+GGG + Chr1:68503299-68503318 MsG0180003805.01.T01:intron 20.0%
!! TGGTTAATTAATTTCTTACT+TGG + Chr1:68503829-68503848 MsG0180003805.01.T01:intron 20.0%
!!! AATAAGTTGAAAACAACTTA+TGG + Chr1:68502506-68502525 MsG0180003805.01.T01:intron 20.0%
!!! AATATACTTCTAAAAACAAG+TGG + Chr1:68504571-68504590 MsG0180003805.01.T01:intron 20.0%
!!! AATTTTATTTACTTTTTGGC+AGG + Chr1:68504960-68504979 MsG0180003805.01.T01:intron 20.0%
!!! ACTTGATTTGCTTAAAAATT+AGG - Chr1:68504771-68504790 None:intergenic 20.0%
!!! ATATACTTCTAAAAACAAGT+GGG + Chr1:68504572-68504591 MsG0180003805.01.T01:intron 20.0%
!!! GAGATAAAGTTGATATTTAT+AGG + Chr1:68503207-68503226 MsG0180003805.01.T01:intron 20.0%
!!! TAATTTTGGTACTTTGTTAT+CGG + Chr1:68502819-68502838 MsG0180003805.01.T01:intron 20.0%
!!! TGTTTGTTCTATATGATTTT+AGG + Chr1:68504166-68504185 MsG0180003805.01.T01:intron 20.0%
!!! TTCTCATGTTTTTTATGATT+AGG + Chr1:68502266-68502285 MsG0180003805.01.T01:intron 20.0%
! AAGAAATTATTTGCAGCAAA+TGG + Chr1:68503459-68503478 MsG0180003805.01.T01:intron 25.0%
! AAGGAAATACTATGGTTTAT+AGG - Chr1:68504059-68504078 None:intergenic 25.0%
! ACATGAGAAACTTAAACAAT+AGG - Chr1:68502255-68502274 None:intergenic 25.0%
! AGTCTGTTTAGAAAAGTTTA+CGG - Chr1:68502550-68502569 None:intergenic 25.0%
! ATTAATTAGATGTATTCCAC+AGG + Chr1:68502144-68502163 MsG0180003805.01.T01:intron 25.0%
! CTAAAAGGAAATTAATGCAT+TGG + Chr1:68503298-68503317 MsG0180003805.01.T01:intron 25.0%
! CTAAACTTCTTCAAAAACTA+AGG - Chr1:68505233-68505252 None:intergenic 25.0%
! TAAGTTGTGAAGAATTTACT+TGG + Chr1:68505396-68505415 MsG0180003805.01.T01:intron 25.0%
! TACAATAGCAAGATGATATT+TGG + Chr1:68503058-68503077 MsG0180003805.01.T01:intron 25.0%
! TGATATTTATAGGTTATTGC+CGG + Chr1:68503217-68503236 MsG0180003805.01.T01:intron 25.0%
! TTCTTACTTGGTTATTTACA+TGG + Chr1:68503841-68503860 MsG0180003805.01.T01:intron 25.0%
! TTGATTATATTTGATGTCCA+TGG + Chr1:68502982-68503001 MsG0180003805.01.T01:intron 25.0%
! TTGGATGTTTATTAATGGAA+TGG + Chr1:68503154-68503173 MsG0180003805.01.T01:intron 25.0%
! TTTCCTTCTTAAGAGTTATA+AGG + Chr1:68504072-68504091 MsG0180003805.01.T01:intron 25.0%
! TTTCTTTAAATTCAGCACAA+CGG - Chr1:68503445-68503464 None:intergenic 25.0%
!! ACATAGAAGCAGTAATTTTT+GGG - Chr1:68504301-68504320 None:intergenic 25.0%
!! ATAGAAAGTAAGAGTATAGA+TGG + Chr1:68504868-68504887 MsG0180003805.01.T01:intron 25.0%
!! TACATAGAAGCAGTAATTTT+TGG - Chr1:68504302-68504321 None:intergenic 25.0%
!! TAGAAAGTAAGAGTATAGAT+GGG + Chr1:68504869-68504888 MsG0180003805.01.T01:intron 25.0%
!!! AAAATGAAGCTTTTGTTTTG+CGG + Chr1:68503104-68503123 MsG0180003805.01.T01:intron 25.0%
!!! AACTTTCTTTAAGACATAGA+GGG - Chr1:68505321-68505340 None:intergenic 25.0%
!!! AATTTTGGTACTTTGTTATC+GGG + Chr1:68502820-68502839 MsG0180003805.01.T01:intron 25.0%
!!! ACAAATTGGTGCATTTTAAT+TGG + Chr1:68503135-68503154 MsG0180003805.01.T01:intron 25.0%
!!! ACACGATTTTTGTTTTATCT+AGG + Chr1:68504678-68504697 MsG0180003805.01.T01:intron 25.0%
!!! ATTTTTTCTAAAGAACACGT+GGG + Chr1:68504439-68504458 MsG0180003805.01.T01:intron 25.0%
!!! CAGTGGTTTTATTTTAGTAT+TGG + Chr1:68503027-68503046 MsG0180003805.01.T01:intron 25.0%
!!! TATTTTTTCTAAAGAACACG+TGG + Chr1:68504438-68504457 MsG0180003805.01.T01:intron 25.0%
!!! TTTGTTTTGATACTTCCTTT+TGG - Chr1:68505143-68505162 None:intergenic 25.0%
AAGTTGCACATATGATTCTA+CGG + Chr1:68504895-68504914 MsG0180003805.01.T01:intron 30.0%
ACAGGCATAGAAAAAGAAAT+TGG + Chr1:68502162-68502181 MsG0180003805.01.T01:CDS 30.0%
ACTATTAATTCCTAAGGGAT+CGG - Chr1:68503898-68503917 None:intergenic 30.0%
ACTTCTTCAAAAACTAAGGA+TGG - Chr1:68505229-68505248 None:intergenic 30.0%
AGTAATTACATTACCTCTAG+TGG - Chr1:68502026-68502045 None:intergenic 30.0%
ATAAAGAAAACTGCTAAGAC+GGG + Chr1:68502309-68502328 MsG0180003805.01.T01:CDS 30.0%
ATACAGTAAGGGATTATAAG+TGG - Chr1:68502122-68502141 None:intergenic 30.0%
ATGTATGGTAAACATAGATG+TGG - Chr1:68505446-68505465 None:intergenic 30.0%
CAACCTTATAACTCTTAAGA+AGG - Chr1:68504078-68504097 None:intergenic 30.0%
CATCACTACAGAGTAATTTA+TGG - Chr1:68503401-68503420 None:intergenic 30.0%
CTAGCACTATTAATTCCTAA+GGG - Chr1:68503903-68503922 None:intergenic 30.0%
CTCTTAAGAAGGAAATACTA+TGG - Chr1:68504067-68504086 None:intergenic 30.0%
GAAAATCTCAGCTATACATT+GGG + Chr1:68503804-68503823 MsG0180003805.01.T01:intron 30.0%
GATAAAGAAAACTGCTAAGA+CGG + Chr1:68502308-68502327 MsG0180003805.01.T01:CDS 30.0%
GCTAATAGCTAAATTGACAA+GGG + Chr1:68504822-68504841 MsG0180003805.01.T01:intron 30.0%
GGAAACTTTGCAATCTTAAA+AGG - Chr1:68503243-68503262 None:intergenic 30.0%
GTACATTAGCAAATGTATCT+AGG + Chr1:68504596-68504615 MsG0180003805.01.T01:intron 30.0%
GTTGTATCATGCATAAGTAT+TGG + Chr1:68504221-68504240 MsG0180003805.01.T01:intron 30.0%
TACCACAAACATAAAGACAT+AGG + Chr1:68504274-68504293 MsG0180003805.01.T01:intron 30.0%
TAGTAATCCATAATCCAGTT+AGG + Chr1:68503965-68503984 MsG0180003805.01.T01:intron 30.0%
TATCTTGTTCCTAGTGATTT+AGG + Chr1:68502358-68502377 MsG0180003805.01.T01:intron 30.0%
TATTAGCTAGTTACACCTTT+AGG - Chr1:68504418-68504437 None:intergenic 30.0%
TCACAAGAAATTCACATGTA+TGG - Chr1:68505461-68505480 None:intergenic 30.0%
TGAAAATCTCAGCTATACAT+TGG + Chr1:68503803-68503822 MsG0180003805.01.T01:intron 30.0%
! AATTTCTTTTTCTATGCCTG+TGG - Chr1:68502163-68502182 None:intergenic 30.0%
! CAATTTTGTGGTATTCCTAA+AGG + Chr1:68504400-68504419 MsG0180003805.01.T01:intron 30.0%
! CTGGAACTCTTGAATAATTT+TGG - Chr1:68503500-68503519 None:intergenic 30.0%
! GAGATTGTTCTTTTGCTAAA+AGG + Chr1:68503283-68503302 MsG0180003805.01.T01:intron 30.0%
!! ACTTTCTTTAAGACATAGAG+GGG - Chr1:68505320-68505339 None:intergenic 30.0%
!! ATTTTGGTACTTTGTTATCG+GGG + Chr1:68502821-68502840 MsG0180003805.01.T01:intron 30.0%
!! GAACTTTCTTTAAGACATAG+AGG - Chr1:68505322-68505341 None:intergenic 30.0%
!! GTTGTTTTCATAAGTCATCT+TGG + Chr1:68502471-68502490 MsG0180003805.01.T01:intron 30.0%
!!! ATAATTTTGGCTTGCTTTGT+AGG - Chr1:68503487-68503506 None:intergenic 30.0%
!!! CTAGCTTACTTGTTTTAAAG+TGG - Chr1:68504342-68504361 None:intergenic 30.0%
!!! TATAGTCCCACTTGTTTTTT+TGG + Chr1:68504487-68504506 MsG0180003805.01.T01:intron 30.0%
!!! TTCTGTTTTATTTACACTCC+TGG + Chr1:68505102-68505121 MsG0180003805.01.T01:intron 30.0%
AACTAAGGATGGAGGAAAAA+TGG - Chr1:68505218-68505237 None:intergenic 35.0%
ACGCTGCATAAATTGACATT+AGG + Chr1:68503373-68503392 MsG0180003805.01.T01:intron 35.0%
ACTTTGCAATCTTAAAAGGC+CGG - Chr1:68503239-68503258 None:intergenic 35.0%
AGAATACTGAAAATGTCAGC+AGG + Chr1:68505162-68505181 MsG0180003805.01.T01:CDS 35.0%
AGGAAATTAATGCATTGGGA+AGG + Chr1:68503303-68503322 MsG0180003805.01.T01:intron 35.0%
ATCTGACAAATCAGGTACTA+AGG + Chr1:68503700-68503719 MsG0180003805.01.T01:intron 35.0%
ATGTCAATTTATGCAGCGTT+AGG - Chr1:68503372-68503391 None:intergenic 35.0%
ATTATCCAAGACATCATCGA+TGG - Chr1:68503655-68503674 None:intergenic 35.0%
CAACAAATTGCTAACCTTCA+AGG + Chr1:68502724-68502743 MsG0180003805.01.T01:CDS 35.0%
CCTAGCACTATTAATTCCTA+AGG - Chr1:68503904-68503923 None:intergenic 35.0%
CCTATGCTTATTATTCATGC+AGG + Chr1:68502866-68502885 MsG0180003805.01.T01:intron 35.0%
CCTGCATGAATAATAAGCAT+AGG - Chr1:68502869-68502888 None:intergenic 35.0%
CCTTAGGAATTAATAGTGCT+AGG + Chr1:68503901-68503920 MsG0180003805.01.T01:intron 35.0%
CTTCTCGATGTCATCAATTT+CGG - Chr1:68505552-68505571 None:intergenic 35.0%
GACAGTAAGAAAATGCAGTA+TGG - Chr1:68505194-68505213 None:intergenic 35.0%
GCAATGGCATCAAATTTCAA+AGG - Chr1:68505525-68505544 None:intergenic 35.0%
GGCTAATAGCTAAATTGACA+AGG + Chr1:68504821-68504840 MsG0180003805.01.T01:intron 35.0%
GGTAAACATAGATGTGGATT+CGG - Chr1:68505440-68505459 None:intergenic 35.0%
GTACCAAAATTAGCATCAGA+TGG - Chr1:68502811-68502830 None:intergenic 35.0%
GTCCTATGTCTTTATGTTTG+TGG - Chr1:68504279-68504298 None:intergenic 35.0%
TAAAATATTAGCCAGGCAGT+TGG + Chr1:68502690-68502709 MsG0180003805.01.T01:CDS 35.0%
TAAAGTGGCAGTACATTACT+TGG - Chr1:68504327-68504346 None:intergenic 35.0%
TAAATTCAGCACAACGGAAA+TGG - Chr1:68503439-68503458 None:intergenic 35.0%
TAACACACGTAGAACAACAT+AGG + Chr1:68504118-68504137 MsG0180003805.01.T01:intron 35.0%
TCTTCAAAAACTAAGGATGG+AGG - Chr1:68505226-68505245 None:intergenic 35.0%
TGATGTCTTGGATAATGATG+AGG + Chr1:68503659-68503678 MsG0180003805.01.T01:CDS 35.0%
TGCAGCAATGTATCATGTAT+CGG - Chr1:68503009-68503028 None:intergenic 35.0%
TTAATAGTGCTAGGAAATGC+AGG + Chr1:68503910-68503929 MsG0180003805.01.T01:intron 35.0%
TTTCCAATGAATGATCGTGA+AGG + Chr1:68503258-68503277 MsG0180003805.01.T01:intron 35.0%
! CTGAGATTTTCAGCAATTCT+GGG - Chr1:68503795-68503814 None:intergenic 35.0%
! GCAATTCTGGGAAATTAATC+AGG - Chr1:68503783-68503802 None:intergenic 35.0%
! GCTAGCAAGTTACAATTTTG+TGG + Chr1:68504388-68504407 MsG0180003805.01.T01:intron 35.0%
! TTTAAAAGAGTACAGACGAC+TGG - Chr1:68505056-68505075 None:intergenic 35.0%
! TTTCAATCTTTTGCAGAGAG+AGG - Chr1:68503087-68503106 None:intergenic 35.0%
!! CTTTCTTTAAGACATAGAGG+GGG - Chr1:68505319-68505338 None:intergenic 35.0%
!! TTTCTTTAAGACATAGAGGG+GGG - Chr1:68505318-68505337 None:intergenic 35.0%
!! TTTTGGTACTTTGTTATCGG+GGG + Chr1:68502822-68502841 MsG0180003805.01.T01:intron 35.0%
ACAGATTCTGCCATTAGATG+TGG - Chr1:68503600-68503619 None:intergenic 40.0%
ACGCAGTCCAAAAAAACAAG+TGG - Chr1:68504497-68504516 None:intergenic 40.0%
CAAACAGAGCCTAAATCACT+AGG - Chr1:68502370-68502389 None:intergenic 40.0%
CATCCTTCACGATCATTCAT+TGG - Chr1:68503264-68503283 None:intergenic 40.0%
CCAGAAACAATCAGCACAAA+TGG + Chr1:68503516-68503535 MsG0180003805.01.T01:CDS 40.0%
CGCAGTCCAAAAAAACAAGT+GGG - Chr1:68504496-68504515 None:intergenic 40.0%
CTAGTGATTTAGGCTCTGTT+TGG + Chr1:68502368-68502387 MsG0180003805.01.T01:intron 40.0%
CTGCGACTAAAATATTAGCC+AGG + Chr1:68502683-68502702 MsG0180003805.01.T01:CDS 40.0%
CTTGTTCAAGGTTAAGGGTT+AGG + Chr1:68503185-68503204 MsG0180003805.01.T01:intron 40.0%
GACAGAAGATCTGACAAATC+AGG + Chr1:68503692-68503711 MsG0180003805.01.T01:CDS 40.0%
GCAGCAATGTATCATGTATC+GGG - Chr1:68503008-68503027 None:intergenic 40.0%
GGCAATGGAAGCTATGAATA+AGG + Chr1:68502938-68502957 MsG0180003805.01.T01:CDS 40.0%
GTTCGTGTTTCCACATCTAA+TGG + Chr1:68503587-68503606 MsG0180003805.01.T01:intron 40.0%
TACATGATACATTGCTGCAG+TGG + Chr1:68503010-68503029 MsG0180003805.01.T01:intron 40.0%
TGAGTCACCTAACTGGATTA+TGG - Chr1:68503975-68503994 None:intergenic 40.0%
TTGCACATATGATTCTACGG+AGG + Chr1:68504898-68504917 MsG0180003805.01.T01:intron 40.0%
TTGCGGAAGGTCTTACAAAT+TGG + Chr1:68503121-68503140 MsG0180003805.01.T01:intron 40.0%
! CCAAGCTTGTTCAAGGTTAA+GGG + Chr1:68503180-68503199 MsG0180003805.01.T01:intron 40.0%
! GCTGAGATTTTCAGCAATTC+TGG - Chr1:68503796-68503815 None:intergenic 40.0%
! GGTCCATCTGATGCTAATTT+TGG + Chr1:68502805-68502824 MsG0180003805.01.T01:intron 40.0%
! TATCATCATGCACCTTTTGC+AGG + Chr1:68502660-68502679 MsG0180003805.01.T01:intron 40.0%
! TCCAAGCTTGTTCAAGGTTA+AGG + Chr1:68503179-68503198 MsG0180003805.01.T01:intron 40.0%
! TTTGGTACTTTGTTATCGGG+GGG + Chr1:68502823-68502842 MsG0180003805.01.T01:intron 40.0%
!! CCATTTGTGCTGATTGTTTC+TGG - Chr1:68503519-68503538 None:intergenic 40.0%
!!! TGAAGCTTTTGTTTTGCGGA+AGG + Chr1:68503108-68503127 MsG0180003805.01.T01:intron 40.0%
AACTGCTAAGACGGGAAATG+AGG + Chr1:68502317-68502336 MsG0180003805.01.T01:CDS 45.0%
ACACAGGTACATTGCTTGAG+TGG + Chr1:68502781-68502800 MsG0180003805.01.T01:intron 45.0%
AGATGCCATCGATGATGTCT+TGG + Chr1:68503647-68503666 MsG0180003805.01.T01:CDS 45.0%
ATCGGAACACAGGATGTTAC+AGG - Chr1:68503880-68503899 None:intergenic 45.0%
ATGTATCATGTATCGGGCCA+TGG - Chr1:68503002-68503021 None:intergenic 45.0%
CAGTTATCAGCAGCTCCAAA+AGG + Chr1:68505125-68505144 MsG0180003805.01.T01:intron 45.0%
CATCAATTTCGGAGCTGCAA+TGG - Chr1:68505541-68505560 None:intergenic 45.0%
CCCTTAACCTTGAACAAGCT+TGG - Chr1:68503183-68503202 None:intergenic 45.0%
CTTACTAGCACCTTTCATGC+CGG - Chr1:68502919-68502938 None:intergenic 45.0%
GTTCTACGTGTGTTATCCCT+GGG - Chr1:68504113-68504132 None:intergenic 45.0%
TATTAGCCAGGCAGTTGGTT+AGG + Chr1:68502695-68502714 MsG0180003805.01.T01:CDS 45.0%
TCTCAGCTATACATTGGGCA+TGG + Chr1:68503809-68503828 MsG0180003805.01.T01:intron 45.0%
TGGCAGGTACTCGATGAAAT+CGG + Chr1:68504976-68504995 MsG0180003805.01.T01:intron 45.0%
TGTTCTACGTGTGTTATCCC+TGG - Chr1:68504114-68504133 None:intergenic 45.0%
TTCTACGTGTGTTATCCCTG+GGG - Chr1:68504112-68504131 None:intergenic 45.0%
TTTGAGCTCGACTACCTTGA+AGG - Chr1:68502741-68502760 None:intergenic 45.0%
! ATGGTGTCCAAGCTTGTTCA+AGG + Chr1:68503173-68503192 MsG0180003805.01.T01:intron 45.0%
! GTTGCATTTTATGCACCCCA+GGG + Chr1:68504094-68504113 MsG0180003805.01.T01:intron 45.0%
! TTGGTACTTTGTTATCGGGG+GGG + Chr1:68502824-68502843 MsG0180003805.01.T01:intron 45.0%
! TTTTAGTCGCAGCCTGCAAA+AGG - Chr1:68502675-68502694 None:intergenic 45.0%
!! ATGTTGCTTCACAGGTTGGT+TGG + Chr1:68505004-68505023 MsG0180003805.01.T01:intron 45.0%
!! GAAAGGTGCTAGTAAGGCAA+TGG + Chr1:68502923-68502942 MsG0180003805.01.T01:CDS 45.0%
!! GCACAGTCTTCTATTTCTGC+CGG + Chr1:68502897-68502916 MsG0180003805.01.T01:CDS 45.0%
!! TCTATTTCTGCCGGCATGAA+AGG + Chr1:68502906-68502925 MsG0180003805.01.T01:CDS 45.0%
!! TGATGACATCGAGAAGCGTT+TGG + Chr1:68505556-68505575 MsG0180003805.01.T01:CDS 45.0%
!!! GTGGGAGTATTTTAGTCAGC+TGG + Chr1:68504457-68504476 MsG0180003805.01.T01:intron 45.0%
!!! AAAAATTTAAAGTATTTTTA+AGG - Chr1:68504797-68504816 None:intergenic 5.0%
!!! TTAAAAATACTTTAAATTTT+TGG + Chr1:68504796-68504815 MsG0180003805.01.T01:intron 5.0%
!!! TTTACATTTTTTTTTTTTTT+TGG + Chr1:68502086-68502105 MsG0180003805.01.T01:intron 5.0%
AGGCATAGCAACTCACACAC+AGG + Chr1:68502765-68502784 MsG0180003805.01.T01:CDS 50.0%
ATCCTGTGTTCCGATCCCTT+AGG + Chr1:68503885-68503904 MsG0180003805.01.T01:intron 50.0%
CAGGTACATTGCTTGAGTGG+TGG + Chr1:68502784-68502803 MsG0180003805.01.T01:intron 50.0%
GGTAGTCGAGCTCAAATGAG+AGG + Chr1:68502745-68502764 MsG0180003805.01.T01:CDS 50.0%
TCTCAGCTGAGTCACCTAAC+TGG - Chr1:68503982-68504001 None:intergenic 50.0%
TTCCTAAGGGATCGGAACAC+AGG - Chr1:68503890-68503909 None:intergenic 50.0%
! GGTTGCATTTTATGCACCCC+AGG + Chr1:68504093-68504112 MsG0180003805.01.T01:intron 50.0%
! GTGGATGTTGCTTCACAGGT+TGG + Chr1:68505000-68505019 MsG0180003805.01.T01:intron 50.0%
! TACTTTGTTATCGGGGGGGT+GGG + Chr1:68502828-68502847 MsG0180003805.01.T01:intron 50.0%
!! CGGCATGAAAGGTGCTAGTA+AGG + Chr1:68502917-68502936 MsG0180003805.01.T01:CDS 50.0%
!! TGACATCGAGAAGCGTTTGG+CGG + Chr1:68505559-68505578 MsG0180003805.01.T01:CDS 50.0%
AGACATAGAGGGGGGACTGT+TGG - Chr1:68505310-68505329 None:intergenic 55.0%
CCAGGCAGTTGGTTAGGCTT+AGG + Chr1:68502701-68502720 MsG0180003805.01.T01:CDS 55.0%
CCTAAGCCTAACCAACTGCC+TGG - Chr1:68502704-68502723 None:intergenic 55.0%
GGGGGACTGTTGGAATGGAT+GGG - Chr1:68505300-68505319 None:intergenic 55.0%
GGTACTCGATGAAATCGGCG+TGG + Chr1:68504981-68505000 MsG0180003805.01.T01:intron 55.0%
TAGAGGGGGGACTGTTGGAA+TGG - Chr1:68505305-68505324 None:intergenic 55.0%
TGGAGCTGCTGATAACTGCC+AGG - Chr1:68505123-68505142 None:intergenic 55.0%
! GTACTTTGTTATCGGGGGGG+TGG + Chr1:68502827-68502846 MsG0180003805.01.T01:intron 55.0%
GGGGGGACTGTTGGAATGGA+TGG - Chr1:68505301-68505320 None:intergenic 60.0%
! CGGCGTGGATGTTGCTTCAC+AGG + Chr1:68504996-68505015 MsG0180003805.01.T01:CDS 60.0%
CGCGCGCGCACACACACACT+TGG + Chr1:68504644-68504663 MsG0180003805.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 68502020 68505598 68502020 ID=MsG0180003805.01;Name=MsG0180003805.01
Chr1 mRNA 68502020 68505598 68502020 ID=MsG0180003805.01.T01;Parent=MsG0180003805.01;Name=MsG0180003805.01.T01;_AED=0.45;_eAED=0.47;_QI=0|0|0|1|1|1|10|0|194
Chr1 exon 68502020 68502031 68502020 ID=MsG0180003805.01.T01:exon:51877;Parent=MsG0180003805.01.T01
Chr1 exon 68502153 68502200 68502153 ID=MsG0180003805.01.T01:exon:51878;Parent=MsG0180003805.01.T01
Chr1 exon 68502288 68502338 68502288 ID=MsG0180003805.01.T01:exon:51879;Parent=MsG0180003805.01.T01
Chr1 exon 68502682 68502786 68502682 ID=MsG0180003805.01.T01:exon:51880;Parent=MsG0180003805.01.T01
Chr1 exon 68502888 68502959 68502888 ID=MsG0180003805.01.T01:exon:51881;Parent=MsG0180003805.01.T01
Chr1 exon 68503475 68503546 68503475 ID=MsG0180003805.01.T01:exon:51882;Parent=MsG0180003805.01.T01
Chr1 exon 68503633 68503713 68503633 ID=MsG0180003805.01.T01:exon:51883;Parent=MsG0180003805.01.T01
Chr1 exon 68504982 68505017 68504982 ID=MsG0180003805.01.T01:exon:51884;Parent=MsG0180003805.01.T01
Chr1 exon 68505128 68505183 68505128 ID=MsG0180003805.01.T01:exon:51885;Parent=MsG0180003805.01.T01
Chr1 exon 68505547 68505598 68505547 ID=MsG0180003805.01.T01:exon:51886;Parent=MsG0180003805.01.T01
Chr1 CDS 68502020 68502031 68502020 ID=MsG0180003805.01.T01:cds;Parent=MsG0180003805.01.T01
Chr1 CDS 68502153 68502200 68502153 ID=MsG0180003805.01.T01:cds;Parent=MsG0180003805.01.T01
Chr1 CDS 68502288 68502338 68502288 ID=MsG0180003805.01.T01:cds;Parent=MsG0180003805.01.T01
Chr1 CDS 68502682 68502786 68502682 ID=MsG0180003805.01.T01:cds;Parent=MsG0180003805.01.T01
Chr1 CDS 68502888 68502959 68502888 ID=MsG0180003805.01.T01:cds;Parent=MsG0180003805.01.T01
Chr1 CDS 68503475 68503546 68503475 ID=MsG0180003805.01.T01:cds;Parent=MsG0180003805.01.T01
Chr1 CDS 68503633 68503713 68503633 ID=MsG0180003805.01.T01:cds;Parent=MsG0180003805.01.T01
Chr1 CDS 68504982 68505017 68504982 ID=MsG0180003805.01.T01:cds;Parent=MsG0180003805.01.T01
Chr1 CDS 68505128 68505183 68505128 ID=MsG0180003805.01.T01:cds;Parent=MsG0180003805.01.T01
Chr1 CDS 68505547 68505598 68505547 ID=MsG0180003805.01.T01:cds;Parent=MsG0180003805.01.T01
Gene Sequence

>MsG0180003805.01.T01

ATGCCACTAGAGATGTATTCCACAGGCATAGAAAAAGAAATTGGAGCATTACAACTTGAAGAAAAGAAGCTAGTTGCTGAGATAAAGAAAACTGCTAAGACGGGAAATGAGGCTGCGACTAAAATATTAGCCAGGCAGTTGGTTAGGCTTAGGCAACAAATTGCTAACCTTCAAGGTAGTCGAGCTCAAATGAGAGGCATAGCAACTCACACACAGGCATTGTATGCACAGTCTTCTATTTCTGCCGGCATGAAAGGTGCTAGTAAGGCAATGGAAGCTATGAATAAGCAAATGGCGCCTACAAAGCAAGCCAAAATTATTCAAGAGTTCCAGAAACAATCAGCACAAATGGATATAACTACTGAAATGATGTCAGATGCCATCGATGATGTCTTGGATAATGATGAGGCTGAAGACGAGACAGAAGATCTGACAAATCAGGTACTCGATGAAATCGGCGTGGATGTTGCTTCACAGTTATCAGCAGCTCCAAAAGGAAGTATCAAAACAAAGAATACTGAAAATGTCAGCAGCTCCGAAATTGATGACATCGAGAAGCGTTTGGCGGCCCTTAGAAACCCCTAA
Protein sequence

>MsG0180003805.01.T01

MPLEMYSTGIEKEIGALQLEEKKLVAEIKKTAKTGNEAATKILARQLVRLRQQIANLQGSRAQMRGIATHTQALYAQSSISAGMKGASKAMEAMNKQMAPTKQAKIIQEFQKQSAQMDITTEMMSDAIDDVLDNDEAEDETEDLTNQVLDEIGVDVASQLSAAPKGSIKTKNTENVSSSEIDDIEKRLAALRNP*