AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031694.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031694.01.T01 MTR_6g029390 52.747 91 41 2 1 90 1 90 1.21e-21 81.3
MsG0680031694.01.T01 MTR_5g098200 51.667 60 29 0 1 60 1 60 3.08e-13 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031694.01.T01 AT5G14602 63.415 41 15 0 1 41 1 41 3.83e-13 59.3
MsG0680031694.01.T01 AT5G18150 82.759 29 5 0 1 29 1 29 8.22e-13 58.5

Find 28 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGATGATTCAAATGGATTT+TGG 0.092886 6:-24757286 MsG0680031694.01.T01:CDS
AGAGAGAGATGTGTCCTTTA+AGG 0.173917 6:-24757959 MsG0680031694.01.T01:exon
ATTGAAATCCTCTAAGAATT+AGG 0.190751 6:-24757194 MsG0680031694.01.T01:exon
ATTGAGTCCCTAATTCTTAG+AGG 0.300143 6:+24757186 None:intergenic
AACACCAAGATGAACCTTAA+AGG 0.300317 6:+24757945 None:intergenic
GATCTTCACCAAAGATGATT+TGG 0.305001 6:-24757878 MsG0680031694.01.T01:CDS
GGTGTTCTTCTCTGCGGTTC+TGG 0.325709 6:-24757928 MsG0680031694.01.T01:CDS
TTGAAATCCTCTAAGAATTA+GGG 0.358948 6:-24757193 MsG0680031694.01.T01:exon
TAAAATTTGTACAAGAATGT+TGG 0.359414 6:+24758052 None:intergenic
ATCACAAGCCAAATCATCTT+TGG 0.376408 6:+24757870 None:intergenic
TTCTGGCAGGTTACTTTGCA+TGG 0.389470 6:-24757911 MsG0680031694.01.T01:CDS
AAAATTTGTACAAGAATGTT+GGG 0.392593 6:+24758053 None:intergenic
TGGCTAGTGGAAGGTATCTA+TGG 0.397595 6:-24757250 MsG0680031694.01.T01:CDS
GTGGGTTCAAGAATAATCTT+TGG 0.400788 6:-24757140 MsG0680031694.01.T01:three_prime_UTR
TTCTTCTCTGCGGTTCTGGC+AGG 0.404540 6:-24757924 MsG0680031694.01.T01:CDS
GTGTCATAAATCCTTGTTTG+TGG 0.445301 6:-24757159 MsG0680031694.01.T01:three_prime_UTR
TTTGTACAAGAATGTTGGGT+TGG 0.456163 6:+24758057 None:intergenic
GTGTCCTTTAAGGTTCATCT+TGG 0.474313 6:-24757949 MsG0680031694.01.T01:CDS
AGGTATCTATGGAGAATTTG+AGG 0.480912 6:-24757239 MsG0680031694.01.T01:CDS
TGTCATAAATCCTTGTTTGT+GGG 0.513684 6:-24757158 MsG0680031694.01.T01:three_prime_UTR
GGTATCTATGGAGAATTTGA+GGG 0.560940 6:-24757238 MsG0680031694.01.T01:CDS
GGCTTGTGATGATTCAATTG+AGG 0.570775 6:-24757857 MsG0680031694.01.T01:CDS
AGGTTACTTTGCATGGAGAA+CGG 0.580884 6:-24757904 MsG0680031694.01.T01:CDS
GTATTCGTTGACATGGCTAG+TGG 0.585469 6:-24757263 MsG0680031694.01.T01:CDS
TATTCTTGAACCCACAAACA+AGG 0.585639 6:+24757148 None:intergenic
CATCTTGGTGTTCTTCTCTG+CGG 0.597461 6:-24757934 MsG0680031694.01.T01:CDS
TCGTTGACATGGCTAGTGGA+AGG 0.632760 6:-24757259 MsG0680031694.01.T01:CDS
GTATCTATGGAGAATTTGAG+GGG 0.679719 6:-24757237 MsG0680031694.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTATATAATTGATCT+TGG - Chr6:24757470-24757489 MsG0680031694.01.T01:intron 10.0%
!!! TTATTTTTAATTTTTGTGTT+TGG - Chr6:24757886-24757905 MsG0680031694.01.T01:CDS 10.0%
!! AAAATTAAAATTCCAAGATT+TGG + Chr6:24757552-24757571 None:intergenic 15.0%
!! AATTAATAACTAATGTTGAT+TGG - Chr6:24757613-24757632 MsG0680031694.01.T01:intron 15.0%
!!! AACATAACTTTAAAAGATAA+AGG + Chr6:24757498-24757517 None:intergenic 15.0%
!!! ATTAATAACTAATGTTGATT+GGG - Chr6:24757614-24757633 MsG0680031694.01.T01:intron 15.0%
!!! TTGATATTTTTTGTTTTATG+AGG + Chr6:24757147-24757166 None:intergenic 15.0%
!! AAAATTTGTACAAGAATGTT+GGG + Chr6:24757188-24757207 None:intergenic 20.0%
!! TAAAATTTGTACAAGAATGT+TGG + Chr6:24757189-24757208 None:intergenic 20.0%
!! TTAGATGATGTTTATTGTTA+AGG - Chr6:24757640-24757659 MsG0680031694.01.T01:intron 20.0%
!!! AAAAATCACATTGTTGTTTA+AGG - Chr6:24757243-24757262 MsG0680031694.01.T01:CDS 20.0%
!!! TAAATCTTGGAATTTTCTTT+GGG - Chr6:24757828-24757847 MsG0680031694.01.T01:CDS 20.0%
!!! TTAAATCTTGGAATTTTCTT+TGG - Chr6:24757827-24757846 MsG0680031694.01.T01:CDS 20.0%
!!! TTGTACAAATTTTAATTCGT+GGG - Chr6:24757195-24757214 MsG0680031694.01.T01:CDS 20.0%
! AGAAAAATCATCTTTCAAGA+AGG - Chr6:24757405-24757424 MsG0680031694.01.T01:intron 25.0%
! AGATGATATGCTAATTGATT+TGG - Chr6:24757735-24757754 MsG0680031694.01.T01:intron 25.0%
! ATTCACCAAAATATGTTGAT+TGG - Chr6:24757782-24757801 MsG0680031694.01.T01:intron 25.0%
! GATGTTTATTGTTAAGGTTT+GGG - Chr6:24757646-24757665 MsG0680031694.01.T01:intron 25.0%
! TGATGTTTATTGTTAAGGTT+TGG - Chr6:24757645-24757664 MsG0680031694.01.T01:intron 25.0%
! TTAATCATGGGAATAATGAT+TGG - Chr6:24757706-24757725 MsG0680031694.01.T01:intron 25.0%
!! AAATCTTGGAATTTTCTTTG+GGG - Chr6:24757829-24757848 MsG0680031694.01.T01:CDS 25.0%
!! AACTCATCAAAATGTTGATT+GGG - Chr6:24757925-24757944 MsG0680031694.01.T01:CDS 25.0%
!! ATTGAAATCCTCTAAGAATT+AGG - Chr6:24758044-24758063 MsG0680031694.01.T01:five_prime_UTR 25.0%
!! CAACAATGTGATTTTTATGA+AGG + Chr6:24757239-24757258 None:intergenic 25.0%
!! GATGATATGCTAATTGATTT+GGG - Chr6:24757736-24757755 MsG0680031694.01.T01:intron 25.0%
!! TAAACCCAATCAACATATTT+TGG + Chr6:24757790-24757809 None:intergenic 25.0%
!! TTCACCAAAATATGTTGATT+GGG - Chr6:24757783-24757802 MsG0680031694.01.T01:intron 25.0%
!! TTGAAATCCTCTAAGAATTA+GGG - Chr6:24758045-24758064 MsG0680031694.01.T01:five_prime_UTR 25.0%
!!! AGATGATTCAAATGGATTTT+GGG - Chr6:24757953-24757972 MsG0680031694.01.T01:CDS 25.0%
!!! ATTTTTTGTTTTATGAGGAG+AGG + Chr6:24757142-24757161 None:intergenic 25.0%
!!! CTTGTACAAATTTTAATTCG+TGG - Chr6:24757194-24757213 MsG0680031694.01.T01:exon 25.0%
!!! GGAAGATTTTGATTTCAAAA+AGG - Chr6:24757426-24757445 MsG0680031694.01.T01:intron 25.0%
!!! GTGTTTGGTTTTTTAATCAT+GGG - Chr6:24757694-24757713 MsG0680031694.01.T01:intron 25.0%
!!! TGTGTTTGGTTTTTTAATCA+TGG - Chr6:24757693-24757712 MsG0680031694.01.T01:intron 25.0%
GAACTCATCAAAATGTTGAT+TGG - Chr6:24757924-24757943 MsG0680031694.01.T01:CDS 30.0%
TCTTTGTGTTTGATTGTGTT+TGG - Chr6:24757679-24757698 MsG0680031694.01.T01:intron 30.0%
! GAGGTTTTCATGTTAAATCT+TGG - Chr6:24757815-24757834 MsG0680031694.01.T01:CDS 30.0%
! TGTCATAAATCCTTGTTTGT+GGG - Chr6:24758080-24758099 MsG0680031694.01.T01:five_prime_UTR 30.0%
!! CAGATGATTCAAATGGATTT+TGG - Chr6:24757952-24757971 MsG0680031694.01.T01:CDS 30.0%
AACACCAAGATGAACCTTAA+AGG + Chr6:24757296-24757315 None:intergenic 35.0%
AGGTATCTATGGAGAATTTG+AGG - Chr6:24757999-24758018 MsG0680031694.01.T01:five_prime_UTR 35.0%
ATCACAAGCCAAATCATCTT+TGG + Chr6:24757371-24757390 None:intergenic 35.0%
ATTGAGTCCCTAATTCTTAG+AGG + Chr6:24758055-24758074 None:intergenic 35.0%
GATCTTCACCAAAGATGATT+TGG - Chr6:24757360-24757379 MsG0680031694.01.T01:intron 35.0%
GGTATCTATGGAGAATTTGA+GGG - Chr6:24758000-24758019 MsG0680031694.01.T01:five_prime_UTR 35.0%
GTATCTATGGAGAATTTGAG+GGG - Chr6:24758001-24758020 MsG0680031694.01.T01:five_prime_UTR 35.0%
GTGGGTTCAAGAATAATCTT+TGG - Chr6:24758098-24758117 MsG0680031694.01.T01:five_prime_UTR 35.0%
GTTGATTGGGTTTATAGATG+AGG - Chr6:24757796-24757815 MsG0680031694.01.T01:intron 35.0%
TAGTCTACGAATCCAAATCT+TGG - Chr6:24757537-24757556 MsG0680031694.01.T01:intron 35.0%
TATTCTTGAACCCACAAACA+AGG + Chr6:24758093-24758112 None:intergenic 35.0%
TTTGTACAAGAATGTTGGGT+TGG + Chr6:24757184-24757203 None:intergenic 35.0%
! GGGTTTTCAGATGATTCAAA+TGG - Chr6:24757945-24757964 MsG0680031694.01.T01:CDS 35.0%
! GTGTCATAAATCCTTGTTTG+TGG - Chr6:24758079-24758098 MsG0680031694.01.T01:five_prime_UTR 35.0%
!! ATTTTGGGTATTCGTTGACA+TGG - Chr6:24757968-24757987 MsG0680031694.01.T01:exon 35.0%
!! TTGTTTTATGAGGAGAGGAA+AGG + Chr6:24757137-24757156 None:intergenic 35.0%
AGAGAGAGATGTGTCCTTTA+AGG - Chr6:24757279-24757298 MsG0680031694.01.T01:CDS 40.0%
GGCTTGTGATGATTCAATTG+AGG - Chr6:24757381-24757400 MsG0680031694.01.T01:intron 40.0%
! AGGTTACTTTGCATGGAGAA+CGG - Chr6:24757334-24757353 MsG0680031694.01.T01:intron 40.0%
!! GTGTCCTTTAAGGTTCATCT+TGG - Chr6:24757289-24757308 MsG0680031694.01.T01:intron 40.0%
!!! CTTGGAATTTTCTTTGGGGT+TGG - Chr6:24757833-24757852 MsG0680031694.01.T01:CDS 40.0%
GTATTCGTTGACATGGCTAG+TGG - Chr6:24757975-24757994 MsG0680031694.01.T01:five_prime_UTR 45.0%
! TTCTGGCAGGTTACTTTGCA+TGG - Chr6:24757327-24757346 MsG0680031694.01.T01:intron 45.0%
!! CATCTTGGTGTTCTTCTCTG+CGG - Chr6:24757304-24757323 MsG0680031694.01.T01:intron 45.0%
!! TGGCTAGTGGAAGGTATCTA+TGG - Chr6:24757988-24758007 MsG0680031694.01.T01:five_prime_UTR 45.0%
!!! AAAAAATAATATTTTTTTGA+AGG + Chr6:24757457-24757476 None:intergenic 5.0%
!!! AAAAATAATATTTTTTTGAA+GGG + Chr6:24757456-24757475 None:intergenic 5.0%
TCGTTGACATGGCTAGTGGA+AGG - Chr6:24757979-24757998 MsG0680031694.01.T01:five_prime_UTR 50.0%
GGTGTTCTTCTCTGCGGTTC+TGG - Chr6:24757310-24757329 MsG0680031694.01.T01:intron 55.0%
! TTCTTCTCTGCGGTTCTGGC+AGG - Chr6:24757314-24757333 MsG0680031694.01.T01:intron 55.0%
Chromosome Type Strat End Strand Name
Chr6 gene 24757103 24758157 24757103 ID=MsG0680031694.01;Name=MsG0680031694.01
Chr6 mRNA 24757103 24758157 24757103 ID=MsG0680031694.01.T01;Parent=MsG0680031694.01;Name=MsG0680031694.01.T01;_AED=0.04;_eAED=0.04;_QI=184|0|0.5|1|0|0|2|92|90
Chr6 exon 24757813 24758157 24757813 ID=MsG0680031694.01.T01:exon:12218;Parent=MsG0680031694.01.T01
Chr6 exon 24757103 24757306 24757103 ID=MsG0680031694.01.T01:exon:12219;Parent=MsG0680031694.01.T01
Chr6 five_prime_UTR 24757974 24758157 24757974 ID=MsG0680031694.01.T01:five_prime_utr;Parent=MsG0680031694.01.T01
Chr6 CDS 24757813 24757973 24757813 ID=MsG0680031694.01.T01:cds;Parent=MsG0680031694.01.T01
Chr6 CDS 24757195 24757306 24757195 ID=MsG0680031694.01.T01:cds;Parent=MsG0680031694.01.T01
Chr6 three_prime_UTR 24757103 24757194 24757103 ID=MsG0680031694.01.T01:three_prime_utr;Parent=MsG0680031694.01.T01
Gene Sequence

>MsG0680031694.01.T01

ATGTGTCCTTTAAGGTTCATCTTGGTGTTCTTCTCTGCGGTTCTGGCAGGTTACTTTGCATGGAGAACGGTTAGATCTTCACCAAAGATGATTTGGCTTGTGATGATTCAATTGAGGAAGAAAAATCATCTTTCAAGAAGGAAGATTTTGATTTCAAAAAGGATGATTCAAATGGATTTTGGGTATTCGTTGACATGGCTAGTGGAAGGTATCTATGGAGAATTTGAGGGGCAAACAAACAAAGATGAAGAATTGAAATCCTCTAAGAATTAG

Protein sequence

>MsG0680031694.01.T01

MCPLRFILVFFSAVLAGYFAWRTVRSSPKMIWLVMIQLRKKNHLSRRKILISKRMIQMDFGYSLTWLVEGIYGEFEGQTNKDEELKSSKN*