AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038443.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038443.01.T01 MTR_7g056133 98.969 97 1 0 1 97 122 218 1.62e-60 184
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038443.01.T01 AT3G07190 67.033 91 30 0 1 91 122 212 6.35e-15 67.4
MsG0780038443.01.T01 AT5G48660 67.347 98 31 1 1 97 46 143 1.21e-14 65.1
MsG0780038443.01.T01 AT5G48660 67.347 98 31 1 1 97 122 219 3.34e-14 65.5
MsG0780038443.01.T01 AT5G42570 39.080 87 53 0 5 91 118 204 1.29e-11 58.5

Find 15 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTTGAGCCTGAAGATTT+TGG 0.137576 7:+46931025 None:intergenic
AACTCTCTTGTCTTTCTCTT+CGG 0.340250 7:+46931131 None:intergenic
TCTAACAGAAGGTTTGAAAA+AGG 0.345623 7:-46931166 MsG0780038443.01.T01:CDS
GGAAGACAACCAAAATCTTC+AGG 0.401724 7:-46931034 MsG0780038443.01.T01:CDS
CAAAATCTTCAGGCTCAAGC+AGG 0.486517 7:-46931024 MsG0780038443.01.T01:CDS
AAATGTCGAGCTCAAAGAAA+AGG 0.491750 7:-46931238 MsG0780038443.01.T01:CDS
GCTTGAATATGACAGACTCT+TGG 0.508233 7:-46931055 MsG0780038443.01.T01:CDS
GAACTTTCAACTCTAACAGA+AGG 0.533214 7:-46931177 MsG0780038443.01.T01:CDS
AAAGGAGGAGAAAGCTTCCA+AGG 0.549039 7:-46931220 MsG0780038443.01.T01:CDS
GGGAGCTTCAAAAGAAGAAG+TGG 0.560241 7:-46931277 MsG0780038443.01.T01:CDS
GAAAGACAAGAGAGTTGAGA+CGG 0.586053 7:-46931124 MsG0780038443.01.T01:CDS
GCAGGGAGGCATAAGAGTTG+AGG 0.597943 7:-46931006 None:intergenic
AAAATCTTCAGGCTCAAGCA+GGG 0.625078 7:-46931023 MsG0780038443.01.T01:CDS
TGTCGAGCTCAAAGAAAAGG+AGG 0.645126 7:-46931235 MsG0780038443.01.T01:CDS
ATCTTCAGGCTCAAGCAGGG+AGG 0.695143 7:-46931020 MsG0780038443.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGGTTTGAAAAAGGTAAAGA+TGG - Chr7:46931130-46931149 MsG0780038443.01.T01:CDS 30.0%
TCTAACAGAAGGTTTGAAAA+AGG - Chr7:46931121-46931140 MsG0780038443.01.T01:CDS 30.0%
AAATGTCGAGCTCAAAGAAA+AGG - Chr7:46931049-46931068 MsG0780038443.01.T01:CDS 35.0%
AACTCTCTTGTCTTTCTCTT+CGG + Chr7:46931159-46931178 None:intergenic 35.0%
GAACTTTCAACTCTAACAGA+AGG - Chr7:46931110-46931129 MsG0780038443.01.T01:CDS 35.0%
!! GCTCGACATTTTCTTTTTTG+AGG + Chr7:46931040-46931059 None:intergenic 35.0%
!! GTAAATCTGCAGCTTGTTTT+TGG + Chr7:46931211-46931230 None:intergenic 35.0%
AAAATCTTCAGGCTCAAGCA+GGG - Chr7:46931264-46931283 MsG0780038443.01.T01:CDS 40.0%
GAAAGACAAGAGAGTTGAGA+CGG - Chr7:46931163-46931182 MsG0780038443.01.T01:CDS 40.0%
GCTTGAATATGACAGACTCT+TGG - Chr7:46931232-46931251 MsG0780038443.01.T01:CDS 40.0%
GGAAGACAACCAAAATCTTC+AGG - Chr7:46931253-46931272 MsG0780038443.01.T01:CDS 40.0%
!! CTTCAGCTGTTTTATCTCCT+TGG + Chr7:46931087-46931106 None:intergenic 40.0%
!!! AATCTGCAGCTTGTTTTTGG+AGG + Chr7:46931208-46931227 None:intergenic 40.0%
!!! ATCTGCAGCTTGTTTTTGGA+GGG + Chr7:46931207-46931226 None:intergenic 40.0%
AAAGGAGGAGAAAGCTTCCA+AGG - Chr7:46931067-46931086 MsG0780038443.01.T01:CDS 45.0%
CAAAATCTTCAGGCTCAAGC+AGG - Chr7:46931263-46931282 MsG0780038443.01.T01:CDS 45.0%
GGGAGCTTCAAAAGAAGAAG+TGG - Chr7:46931010-46931029 MsG0780038443.01.T01:CDS 45.0%
TGTCGAGCTCAAAGAAAAGG+AGG - Chr7:46931052-46931071 MsG0780038443.01.T01:CDS 45.0%
! CTGCTTGAGCCTGAAGATTT+TGG + Chr7:46931265-46931284 None:intergenic 45.0%
ATCTTCAGGCTCAAGCAGGG+AGG - Chr7:46931267-46931286 MsG0780038443.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 46931008 46931301 46931008 ID=MsG0780038443.01;Name=MsG0780038443.01
Chr7 mRNA 46931008 46931301 46931008 ID=MsG0780038443.01.T01;Parent=MsG0780038443.01;Name=MsG0780038443.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|97
Chr7 exon 46931008 46931301 46931008 ID=MsG0780038443.01.T01:exon:4852;Parent=MsG0780038443.01.T01
Chr7 CDS 46931008 46931301 46931008 ID=MsG0780038443.01.T01:cds;Parent=MsG0780038443.01.T01
Gene Sequence

>MsG0780038443.01.T01

ATGGGAGCTTCAAAAGAAGAAGTGGAGAACCTCAAAAAAGAAAATGTCGAGCTCAAAGAAAAGGAGGAGAAAGCTTCCAAGGAGATAAAACAGCTGAAGAAAGAACTTTCAACTCTAACAGAAGGTTTGAAAAAGGTAAAGATGGAATCCGAAGAGAAAGACAAGAGAGTTGAGACGGCTGATGCTCATGTTGCTTCCCTCCAAAAACAAGCTGCAGATTTACTGCTTGAATATGACAGACTCTTGGAAGACAACCAAAATCTTCAGGCTCAAGCAGGGAGGCATAAGAGTTGA

Protein sequence

>MsG0780038443.01.T01

MGASKEEVENLKKENVELKEKEEKASKEIKQLKKELSTLTEGLKKVKMESEEKDKRVETADAHVASLQKQAADLLLEYDRLLEDNQNLQAQAGRHKS*