AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880042129.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042129.01.T01 MTR_8g012380 96.875 32 1 0 4 35 58 89 1.19e-15 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042129.01.T01 AT3G25220 81.250 32 6 0 4 35 60 91 2.85e-11 57.0

Find 15 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCATCGTTGGATTCTCTTT+TGG 0.252016 8:+4157059 None:intergenic
AAATATTTAATTGTCTTTGC+AGG 0.304612 8:-4157961 None:intergenic
CCACCAAGCTCAAAATCAAT+TGG 0.428908 8:+4157888 None:intergenic
AATATTTAATTGTCTTTGCA+GGG 0.474811 8:-4157960 None:intergenic
GCAAAAGTTTACCTCACAAA+GGG 0.505987 8:-4157157 MsG0880042129.01.T01:CDS
ACGCGAAGTCTTCCCATCGT+TGG 0.520687 8:+4157047 None:intergenic
AGCAAAAGTTTACCTCACAA+AGG 0.577448 8:-4157158 MsG0880042129.01.T01:CDS
CTTCGTGATGTTATTACTCG+CGG 0.587339 8:-4157021 MsG0880042129.01.T01:CDS
CCAAAAGAGAATCCAACGAT+GGG 0.597201 8:-4157059 MsG0880042129.01.T01:CDS
AGAATCAAATACAGTTCCAT+CGG 0.607844 8:+4157929 None:intergenic
GCCAAAAGAGAATCCAACGA+TGG 0.613654 8:-4157060 MsG0880042129.01.T01:CDS
GGTGGTGGTCAAGTGATCAA+AGG 0.629184 8:-4157870 MsG0880042129.01.T01:CDS
TTGCAGGGAAAACTCACCGA+TGG 0.633072 8:-4157945 MsG0880042129.01.T01:CDS
CTGTTCGTGATCCCTTTGTG+AGG 0.652527 8:+4157146 None:intergenic
AAAAGAACGAGAGTTATGTG+TGG 0.717867 8:+4157836 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGCTCAAATTTAATTAATT+TGG + Chr8:4157391-4157410 None:intergenic 15.0%
!! ATGATTTAATAGTTAAGACA+GGG - Chr8:4157567-4157586 MsG0880042129.01.T01:intron 20.0%
!! CTTAACTATTAAATCATATC+TGG + Chr8:4157564-4157583 None:intergenic 20.0%
!! TATGATTTAATAGTTAAGAC+AGG - Chr8:4157566-4157585 MsG0880042129.01.T01:intron 20.0%
!! TATTTAATCTAAATCCTTGA+TGG + Chr8:4157533-4157552 None:intergenic 20.0%
!! TTCTACTAAGCATATAATAT+AGG + Chr8:4157450-4157469 None:intergenic 20.0%
!!! AAATTTTCTTAACATCCAAA+AGG + Chr8:4157675-4157694 None:intergenic 20.0%
!!! AATTTTCTTAACATCCAAAA+GGG + Chr8:4157674-4157693 None:intergenic 20.0%
! AGGATATAAACTAGTTCAAA+AGG + Chr8:4157430-4157449 None:intergenic 25.0%
! ATTGCAAAATGAAAGCTAAA+AGG - Chr8:4157206-4157225 MsG0880042129.01.T01:intron 25.0%
! GTTAAAATAGCAGATCATAA+TGG - Chr8:4157752-4157771 MsG0880042129.01.T01:intron 25.0%
AGAATCAAATACAGTTCCAT+CGG + Chr8:4157039-4157058 None:intergenic 30.0%
! ATTCTCTTTTGGCAGAAAAA+AGG + Chr8:4157898-4157917 None:intergenic 30.0%
! CTTAAGATTTTACTCCCTTT+TGG - Chr8:4157657-4157676 MsG0880042129.01.T01:intron 30.0%
!! AATCCAATTGATTTTGAGCT+TGG - Chr8:4157074-4157093 MsG0880042129.01.T01:CDS 30.0%
AAAAGAACGAGAGTTATGTG+TGG + Chr8:4157132-4157151 None:intergenic 35.0%
AACATATAGTTCACGCTCTT+TGG + Chr8:4157735-4157754 None:intergenic 35.0%
ACACCATATCAATCTGAGAA+TGG - Chr8:4157695-4157714 MsG0880042129.01.T01:intron 35.0%
AGCAAAAGTTTACCTCACAA+AGG - Chr8:4157807-4157826 MsG0880042129.01.T01:CDS 35.0%
CACCATATCAATCTGAGAAT+GGG - Chr8:4157696-4157715 MsG0880042129.01.T01:intron 35.0%
GCAAAAGTTTACCTCACAAA+GGG - Chr8:4157808-4157827 MsG0880042129.01.T01:CDS 35.0%
GTCTCCTATGACAAGTAAAA+TGG - Chr8:4157345-4157364 MsG0880042129.01.T01:intron 35.0%
TTCCCATTCTCAGATTGATA+TGG + Chr8:4157701-4157720 None:intergenic 35.0%
! CTCTCGTTCTTTTGAATTTC+TGG - Chr8:4157139-4157158 MsG0880042129.01.T01:CDS 35.0%
!! TCAGCCATTTTACTTGTCAT+AGG + Chr8:4157352-4157371 None:intergenic 35.0%
!!! AAAAGCCTTTTTCGACAAAG+GGG + Chr8:4157620-4157639 None:intergenic 35.0%
!!! ACAAAAGCCTTTTTCGACAA+AGG + Chr8:4157622-4157641 None:intergenic 35.0%
!!! CAAAAGCCTTTTTCGACAAA+GGG + Chr8:4157621-4157640 None:intergenic 35.0%
AACTACATGCAAAGCCATCA+AGG - Chr8:4157516-4157535 MsG0880042129.01.T01:intron 40.0%
AGTAGAAACCTGTGTAAGGA+GGG - Chr8:4157463-4157482 MsG0880042129.01.T01:intron 40.0%
CCAAAAGAGAATCCAACGAT+GGG - Chr8:4157906-4157925 MsG0880042129.01.T01:CDS 40.0%
CCACCAAGCTCAAAATCAAT+TGG + Chr8:4157080-4157099 None:intergenic 40.0%
CTTCGTGATGTTATTACTCG+CGG - Chr8:4157944-4157963 MsG0880042129.01.T01:CDS 40.0%
GCTTAGTAGAAACCTGTGTA+AGG - Chr8:4157459-4157478 MsG0880042129.01.T01:intron 40.0%
GCTTTGCATGTAGTTTCTCT+GGG + Chr8:4157511-4157530 None:intergenic 40.0%
TAATGAAATAGCTGCTGTGC+TGG - Chr8:4157172-4157191 MsG0880042129.01.T01:intron 40.0%
TAGTAGAAACCTGTGTAAGG+AGG - Chr8:4157462-4157481 MsG0880042129.01.T01:intron 40.0%
! CTTTTGGCAGAAAAAAGGAG+TGG + Chr8:4157893-4157912 None:intergenic 40.0%
!! ATTGATTTTGAGCTTGGTGG+TGG - Chr8:4157080-4157099 MsG0880042129.01.T01:CDS 40.0%
!! CCAATTGATTTTGAGCTTGG+TGG - Chr8:4157077-4157096 MsG0880042129.01.T01:CDS 40.0%
!! TGGCTGATTTGTAACATGTG+AGG - Chr8:4157365-4157384 MsG0880042129.01.T01:intron 40.0%
AACAACTACCCTCCTTACAC+AGG + Chr8:4157474-4157493 None:intergenic 45.0%
ATCATTGTAGCTCACGGAGA+GGG - Chr8:4157290-4157309 MsG0880042129.01.T01:intron 45.0%
GCCAAAAGAGAATCCAACGA+TGG - Chr8:4157905-4157924 MsG0880042129.01.T01:CDS 45.0%
GGCTTTGCATGTAGTTTCTC+TGG + Chr8:4157512-4157531 None:intergenic 45.0%
GTCAACCCCTTTGTCGAAAA+AGG - Chr8:4157612-4157631 MsG0880042129.01.T01:intron 45.0%
TGCCAGATCATTGTAGCTCA+CGG - Chr8:4157284-4157303 MsG0880042129.01.T01:intron 45.0%
TGGCAGAAAAAAGGAGTGGT+GGG + Chr8:4157889-4157908 None:intergenic 45.0%
TTGCATGTAGTTTCTCTGGG+TGG + Chr8:4157508-4157527 None:intergenic 45.0%
TTGGCAGAAAAAAGGAGTGG+TGG + Chr8:4157890-4157909 None:intergenic 45.0%
! CCCATCGTTGGATTCTCTTT+TGG + Chr8:4157909-4157928 None:intergenic 45.0%
ACGGAGAGGGTTGTTGATGT+TGG - Chr8:4157303-4157322 MsG0880042129.01.T01:intron 50.0%
CTCCGTGAGCTACAATGATC+TGG + Chr8:4157289-4157308 None:intergenic 50.0%
CTGTTCGTGATCCCTTTGTG+AGG + Chr8:4157822-4157841 None:intergenic 50.0%
GATCATTGTAGCTCACGGAG+AGG - Chr8:4157289-4157308 MsG0880042129.01.T01:intron 50.0%
TTGCAGGGAAAACTCACCGA+TGG - Chr8:4157020-4157039 MsG0880042129.01.T01:CDS 50.0%
! GGTGGTGGTCAAGTGATCAA+AGG - Chr8:4157095-4157114 MsG0880042129.01.T01:CDS 50.0%
ACGCGAAGTCTTCCCATCGT+TGG + Chr8:4157921-4157940 None:intergenic 55.0%
CGACAAAGGGGTTGACTGCA+TGG + Chr8:4157608-4157627 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 4157014 4157973 4157014 ID=MsG0880042129.01;Name=MsG0880042129.01
Chr8 mRNA 4157014 4157973 4157014 ID=MsG0880042129.01.T01;Parent=MsG0880042129.01;Name=MsG0880042129.01.T01;_AED=0.50;_eAED=0.86;_QI=0|0|0|1|0|0|2|0|112
Chr8 exon 4157800 4157973 4157800 ID=MsG0880042129.01.T01:exon:2507;Parent=MsG0880042129.01.T01
Chr8 exon 4157014 4157178 4157014 ID=MsG0880042129.01.T01:exon:2506;Parent=MsG0880042129.01.T01
Chr8 CDS 4157800 4157973 4157800 ID=MsG0880042129.01.T01:cds;Parent=MsG0880042129.01.T01
Chr8 CDS 4157014 4157178 4157014 ID=MsG0880042129.01.T01:cds;Parent=MsG0880042129.01.T01
Gene Sequence

>MsG0880042129.01.T01

TTGTCTTTGCAGGGAAAACTCACCGATGGAACTGTATTTGATTCTAGCTTTGAAAGAAATAATCCAATTGATTTTGAGCTTGGTGGTGGTCAAGTGATCAAAGGTAAGCGCACAGCCACACATAACTCTCGTTCTTTTGAATTTCTGGTTTCATTAGATAATGAAATAGCTGCTCAAAAGTTTACCTCACAAAGGGATCACGAACAGTTATATGAGTATCGAGTAACTGTAGCAGAAATCACTTTTTTCATCCCACCACTCCTTTTTTCTGCCAAAAGAGAATCCAACGATGGGAAGACTTCGCGTTCGCTTCGTGATGTTATTACTCGCGGCAAATAG

Protein sequence

>MsG0880042129.01.T01

LSLQGKLTDGTVFDSSFERNNPIDFELGGGQVIKGKRTATHNSRSFEFLVSLDNEIAAQKFTSQRDHEQLYEYRVTVAEITFFIPPLLFSAKRESNDGKTSRSLRDVITRGK*