Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004497.01.T01 | XP_003591003.1 | 83.893 | 298 | 16 | 3 | 1 | 268 | 262 | 557 | 1.29E-147 | 434 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004497.01.T01 | Q8H181 | 50.196 | 255 | 84 | 7 | 1 | 218 | 310 | 558 | 2.49E-68 | 225 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004497.01.T01 | G7I6V9 | 83.893 | 298 | 16 | 3 | 1 | 268 | 262 | 557 | 6.15e-148 | 434 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0180004497.01.T01 | TF | Trihelix |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000210.01 | MsG0180004497.01 | 0.808134 | 3.595897e-50 | 2.039660e-47 |
| MsG0180003741.01 | MsG0180004497.01 | 0.832065 | 1.169126e-55 | 1.292476e-52 |
| MsG0180004046.01 | MsG0180004497.01 | 0.802935 | 4.430630e-49 | 2.197928e-46 |
| MsG0180004497.01 | MsG0280006937.01 | 0.802676 | 5.010967e-49 | 2.469517e-46 |
| MsG0180004497.01 | MsG0480018200.01 | 0.800227 | 1.591364e-48 | 7.370143e-46 |
| MsG0180004497.01 | MsG0580024443.01 | 0.804413 | 2.186009e-49 | 1.126362e-46 |
| MsG0180004497.01 | MsG0580027655.01 | 0.818412 | 1.986919e-52 | 1.486352e-49 |
| MsG0180004497.01 | MsG0580028570.01 | 0.828450 | 8.930971e-55 | 8.880606e-52 |
| MsG0180004497.01 | MsG0580029310.01 | 0.809477 | 1.855895e-50 | 1.090767e-47 |
| MsG0180004497.01 | MsG0680030784.01 | 0.814770 | 1.301064e-51 | 8.807912e-49 |
| MsG0180004497.01 | MsG0780038847.01 | 0.838982 | 2.080934e-57 | 2.833728e-54 |
| MsG0180004497.01 | MsG0780038948.01 | 0.802956 | 4.386222e-49 | 2.177092e-46 |
| MsG0180004497.01 | MsG0780041259.01 | 0.828052 | 1.114128e-54 | 1.095032e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004497.01.T01 | MTR_1g081180 | 83.893 | 298 | 16 | 3 | 1 | 268 | 262 | 557 | 1.56e-151 | 434 |
| MsG0180004497.01.T01 | MTR_7g114860 | 52.941 | 238 | 83 | 7 | 1 | 218 | 251 | 479 | 3.56e-65 | 211 |
| MsG0180004497.01.T01 | MTR_1g098900 | 35.294 | 255 | 126 | 5 | 1 | 218 | 291 | 543 | 2.27e-43 | 156 |
| MsG0180004497.01.T01 | MTR_1g098900 | 40.000 | 100 | 57 | 2 | 116 | 215 | 46 | 142 | 5.61e-18 | 83.6 |
| MsG0180004497.01.T01 | MTR_2g026725 | 34.510 | 255 | 144 | 5 | 1 | 239 | 125 | 372 | 3.05e-36 | 132 |
| MsG0180004497.01.T01 | MTR_1g098870 | 35.714 | 224 | 129 | 4 | 1 | 218 | 247 | 461 | 9.31e-34 | 129 |
| MsG0180004497.01.T01 | MTR_1g098870 | 40.217 | 92 | 52 | 2 | 127 | 218 | 55 | 143 | 1.90e-15 | 76.3 |
| MsG0180004497.01.T01 | MTR_6g035370 | 59.783 | 92 | 35 | 1 | 127 | 218 | 464 | 553 | 2.55e-29 | 116 |
| MsG0180004497.01.T01 | MTR_6g035370 | 39.796 | 98 | 56 | 2 | 120 | 217 | 48 | 142 | 4.29e-17 | 81.3 |
| MsG0180004497.01.T01 | MTR_1g492790 | 36.090 | 133 | 72 | 4 | 90 | 212 | 68 | 197 | 1.28e-16 | 79.7 |
| MsG0180004497.01.T01 | MTR_1g060990 | 39.785 | 93 | 52 | 3 | 127 | 218 | 131 | 220 | 1.15e-13 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004497.01.T01 | AT5G28300 | 50.196 | 255 | 84 | 7 | 1 | 218 | 310 | 558 | 2.54e-69 | 225 |
| MsG0180004497.01.T01 | AT1G76890 | 40.175 | 229 | 112 | 6 | 1 | 218 | 272 | 486 | 6.69e-43 | 154 |
| MsG0180004497.01.T01 | AT1G76890 | 46.591 | 88 | 44 | 2 | 125 | 212 | 39 | 123 | 6.63e-18 | 83.6 |
| MsG0180004497.01.T01 | AT1G76880 | 35.745 | 235 | 132 | 3 | 1 | 218 | 265 | 497 | 1.33e-38 | 142 |
| MsG0180004497.01.T01 | AT1G76880 | 38.182 | 110 | 65 | 2 | 120 | 229 | 54 | 160 | 1.30e-16 | 79.7 |
| MsG0180004497.01.T01 | AT5G47660 | 37.037 | 216 | 120 | 5 | 1 | 216 | 189 | 388 | 1.18e-28 | 112 |
| MsG0180004497.01.T01 | AT1G33240 | 57.778 | 90 | 36 | 1 | 127 | 216 | 435 | 522 | 4.23e-28 | 113 |
| MsG0180004497.01.T01 | AT1G33240 | 43.820 | 89 | 47 | 2 | 127 | 215 | 62 | 147 | 1.03e-18 | 85.9 |
| MsG0180004497.01.T01 | AT1G33240 | 57.778 | 90 | 36 | 1 | 127 | 216 | 435 | 522 | 4.51e-28 | 113 |
| MsG0180004497.01.T01 | AT1G33240 | 43.820 | 89 | 47 | 2 | 127 | 215 | 62 | 147 | 1.00e-18 | 85.9 |
| MsG0180004497.01.T01 | AT1G33240 | 57.778 | 90 | 36 | 1 | 127 | 216 | 396 | 483 | 5.06e-28 | 113 |
| MsG0180004497.01.T01 | AT3G10000 | 40.000 | 95 | 54 | 2 | 127 | 220 | 88 | 180 | 8.77e-16 | 77.0 |
| MsG0180004497.01.T01 | AT5G03680 | 42.857 | 91 | 48 | 3 | 127 | 216 | 119 | 206 | 6.14e-15 | 74.7 |
| MsG0180004497.01.T01 | AT3G10000 | 36.170 | 94 | 49 | 2 | 127 | 220 | 88 | 170 | 9.16e-13 | 68.2 |
Find 51 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAAGGCTATATGAAGAAAT+AGG | 0.236435 | 1:+78904517 | None:intergenic |
| CTCTCTCGCTGCTCGATATA+AGG | 0.299308 | 1:+78904500 | None:intergenic |
| TCCTTCTTCTTCTACTACTT+TGG | 0.306363 | 1:-78904563 | MsG0180004497.01.T01:CDS |
| AATGCCAACACTTCATCTTT+TGG | 0.317647 | 1:+78904457 | None:intergenic |
| TTTCAAGAGTTTCCGGATTA+GGG | 0.337138 | 1:+78904626 | None:intergenic |
| TCACAAGGAATGTTGGAGTT+AGG | 0.345818 | 1:-78904355 | MsG0180004497.01.T01:CDS |
| GAGCTAGGGTTTACCGGTAT+TGG | 0.348770 | 1:+78904592 | None:intergenic |
| GAACAAAGAGCTTGAGTTGA+TGG | 0.361917 | 1:-78904813 | MsG0180004497.01.T01:CDS |
| AGCAGCGAGAGAGACGATAT+TGG | 0.377007 | 1:-78904490 | MsG0180004497.01.T01:CDS |
| GAAGAAGGAAGGGTTGAGCT+AGG | 0.386310 | 1:+78904577 | None:intergenic |
| CAACAACAAGGCACCACTTT+GGG | 0.393210 | 1:-78904386 | MsG0180004497.01.T01:CDS |
| TTCTTCTTCTACTACTTTGG+TGG | 0.410965 | 1:-78904560 | MsG0180004497.01.T01:CDS |
| AGGGTTGAGCTAGGGTTTAC+CGG | 0.424547 | 1:+78904586 | None:intergenic |
| AGTTTAGTAGAGAAGAAGCT+TGG | 0.425700 | 1:-78904859 | MsG0180004497.01.T01:CDS |
| TTGAGCAATCTTTATAATCA+AGG | 0.426028 | 1:-78904208 | MsG0180004497.01.T01:CDS |
| TAAGGCTATATGAAGAAATA+GGG | 0.437015 | 1:+78904518 | None:intergenic |
| GAGAATTTCACAAGGAATGT+TGG | 0.441400 | 1:-78904362 | MsG0180004497.01.T01:CDS |
| CAACAACAACAATGAAGAAA+AGG | 0.456418 | 1:-78904419 | MsG0180004497.01.T01:CDS |
| CACCTGCACCAGCACCATCT+TGG | 0.460031 | 1:+78904089 | None:intergenic |
| AAGAAGGAAGGGTTGAGCTA+GGG | 0.460441 | 1:+78904578 | None:intergenic |
| TTGCTCAATAGATGAAAGTA+AGG | 0.463276 | 1:+78904223 | None:intergenic |
| CTCAAGTGGGGCCTCCAAGC+TGG | 0.484767 | 1:-78904060 | MsG0180004497.01.T01:CDS |
| CAAAGGATGCTAATAGGAAA+AGG | 0.488346 | 1:-78904267 | MsG0180004497.01.T01:CDS |
| GCAACAACAAGGCACCACTT+TGG | 0.490808 | 1:-78904387 | MsG0180004497.01.T01:CDS |
| GCACATGAACAAGCTATTGC+TGG | 0.507249 | 1:-78904791 | MsG0180004497.01.T01:CDS |
| GCAGGTGAGTCTTCTCAAGT+GGG | 0.514949 | 1:-78904073 | MsG0180004497.01.T01:CDS |
| GCACTCACAAAACCCTAATC+CGG | 0.519084 | 1:-78904638 | MsG0180004497.01.T01:CDS |
| AACCAAGATGGTGCTGGTGC+AGG | 0.525566 | 1:-78904091 | MsG0180004497.01.T01:CDS |
| TGCAGGTGAGTCTTCTCAAG+TGG | 0.532154 | 1:-78904074 | MsG0180004497.01.T01:CDS |
| AAGTAGTAGAAGAAGAAGGA+AGG | 0.534716 | 1:+78904566 | None:intergenic |
| GAGACGATATTGGTAGAAGA+TGG | 0.544390 | 1:-78904480 | MsG0180004497.01.T01:CDS |
| CAAAGAGGTGCAAAGAGAAG+TGG | 0.549838 | 1:-78904318 | MsG0180004497.01.T01:CDS |
| TAGTGAAATTACCAGCTTGG+AGG | 0.560803 | 1:+78904049 | None:intergenic |
| ACCAAAGTAGTAGAAGAAGA+AGG | 0.573800 | 1:+78904562 | None:intergenic |
| ATGGCCAAAAGATGAAGTGT+TGG | 0.578682 | 1:-78904461 | MsG0180004497.01.T01:CDS |
| AAAGAGGTGCAAAGAGAAGT+GGG | 0.586402 | 1:-78904317 | MsG0180004497.01.T01:CDS |
| ATATAGTGAAATTACCAGCT+TGG | 0.588322 | 1:+78904046 | None:intergenic |
| AGTAGTAGAAGAAGAAGGAA+GGG | 0.592188 | 1:+78904567 | None:intergenic |
| GAAATAGGGTTGTTCTTGCG+AGG | 0.598239 | 1:+78904532 | None:intergenic |
| TGTGAAATTCTCTCCCAAAG+TGG | 0.599247 | 1:+78904373 | None:intergenic |
| AGCTTGGAAGAAACAAGAGA+TGG | 0.599559 | 1:-78904843 | MsG0180004497.01.T01:CDS |
| CTTTGGGAGAGAATTTCACA+AGG | 0.610777 | 1:-78904370 | MsG0180004497.01.T01:CDS |
| GATGAAAACCAAGATGGTGC+TGG | 0.618902 | 1:-78904097 | MsG0180004497.01.T01:CDS |
| AACAACAATGAAGAAAAGGA+AGG | 0.621045 | 1:-78904415 | MsG0180004497.01.T01:CDS |
| AGAAAGCAATAACGTGAATG+TGG | 0.625602 | 1:-78904155 | MsG0180004497.01.T01:CDS |
| CAGGTGAGTCTTCTCAAGTG+GGG | 0.628435 | 1:-78904072 | MsG0180004497.01.T01:CDS |
| ACACAAGATGAAAACCAAGA+TGG | 0.647031 | 1:-78904103 | MsG0180004497.01.T01:CDS |
| TGTTGGCATTGATAAACTTG+AGG | 0.669533 | 1:-78904444 | MsG0180004497.01.T01:CDS |
| GATACAAGAGAAGTGCAAAG+AGG | 0.678975 | 1:-78904333 | MsG0180004497.01.T01:CDS |
| GGAAGGAAATAGCAACAACA+AGG | 0.682423 | 1:-78904398 | MsG0180004497.01.T01:CDS |
| GAATGTGGCTGAAGAAAATG+TGG | 0.683811 | 1:-78904140 | MsG0180004497.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATAAGGCTATATGAAGAAAT+AGG | + | Chr1:78904469-78904488 | None:intergenic | 25.0% |
| ! | TAAGGCTATATGAAGAAATA+GGG | + | Chr1:78904468-78904487 | None:intergenic | 25.0% |
| ! | TTGAGCAATCTTTATAATCA+AGG | - | Chr1:78904775-78904794 | MsG0180004497.01.T01:intron | 25.0% |
| AAAAACTCTTCAACCAATAC+CGG | - | Chr1:78904378-78904397 | MsG0180004497.01.T01:CDS | 30.0% | |
| AACAACAATGAAGAAAAGGA+AGG | - | Chr1:78904568-78904587 | MsG0180004497.01.T01:CDS | 30.0% | |
| CAACAACAACAATGAAGAAA+AGG | - | Chr1:78904564-78904583 | MsG0180004497.01.T01:CDS | 30.0% | |
| CAAGTACTTCAGAAAAACAA+AGG | - | Chr1:78904699-78904718 | MsG0180004497.01.T01:intron | 30.0% | |
| GAAAAACAAAGGATGCTAAT+AGG | - | Chr1:78904710-78904729 | MsG0180004497.01.T01:intron | 30.0% | |
| TTGCTCAATAGATGAAAGTA+AGG | + | Chr1:78904763-78904782 | None:intergenic | 30.0% | |
| !!! | AGAGTTTTTTCAAGAGTTTC+CGG | + | Chr1:78904367-78904386 | None:intergenic | 30.0% |
| AAGTAGTAGAAGAAGAAGGA+AGG | + | Chr1:78904420-78904439 | None:intergenic | 35.0% | |
| ACACAAGATGAAAACCAAGA+TGG | - | Chr1:78904880-78904899 | MsG0180004497.01.T01:CDS | 35.0% | |
| ACCAAAGTAGTAGAAGAAGA+AGG | + | Chr1:78904424-78904443 | None:intergenic | 35.0% | |
| AGAAAGCAATAACGTGAATG+TGG | - | Chr1:78904828-78904847 | MsG0180004497.01.T01:CDS | 35.0% | |
| AGTAGTAGAAGAAGAAGGAA+GGG | + | Chr1:78904419-78904438 | None:intergenic | 35.0% | |
| AGTTTAGTAGAGAAGAAGCT+TGG | - | Chr1:78904124-78904143 | MsG0180004497.01.T01:CDS | 35.0% | |
| CAAAGGATGCTAATAGGAAA+AGG | - | Chr1:78904716-78904735 | MsG0180004497.01.T01:intron | 35.0% | |
| GAGAATTTCACAAGGAATGT+TGG | - | Chr1:78904621-78904640 | MsG0180004497.01.T01:CDS | 35.0% | |
| TCCTTCTTCTTCTACTACTT+TGG | - | Chr1:78904420-78904439 | MsG0180004497.01.T01:CDS | 35.0% | |
| TTCTTCTTCTACTACTTTGG+TGG | - | Chr1:78904423-78904442 | MsG0180004497.01.T01:CDS | 35.0% | |
| TTTCAAGAGTTTCCGGATTA+GGG | + | Chr1:78904360-78904379 | None:intergenic | 35.0% | |
| ! | AATGCCAACACTTCATCTTT+TGG | + | Chr1:78904529-78904548 | None:intergenic | 35.0% |
| !! | TGTTGGCATTGATAAACTTG+AGG | - | Chr1:78904539-78904558 | MsG0180004497.01.T01:CDS | 35.0% |
| !! | TTTTCAAGAGTTTCCGGATT+AGG | + | Chr1:78904361-78904380 | None:intergenic | 35.0% |
| AGCTTGGAAGAAACAAGAGA+TGG | - | Chr1:78904140-78904159 | MsG0180004497.01.T01:CDS | 40.0% | |
| AGTGAAGAGACATTGAGCTA+AGG | + | Chr1:78904280-78904299 | None:intergenic | 40.0% | |
| ATGGCCAAAAGATGAAGTGT+TGG | - | Chr1:78904522-78904541 | MsG0180004497.01.T01:CDS | 40.0% | |
| GAATGTGGCTGAAGAAAATG+TGG | - | Chr1:78904843-78904862 | MsG0180004497.01.T01:CDS | 40.0% | |
| GAGACGATATTGGTAGAAGA+TGG | - | Chr1:78904503-78904522 | MsG0180004497.01.T01:CDS | 40.0% | |
| GGAAGGAAATAGCAACAACA+AGG | - | Chr1:78904585-78904604 | MsG0180004497.01.T01:CDS | 40.0% | |
| TCACAAGGAATGTTGGAGTT+AGG | - | Chr1:78904628-78904647 | MsG0180004497.01.T01:CDS | 40.0% | |
| TGTGAAATTCTCTCCCAAAG+TGG | + | Chr1:78904613-78904632 | None:intergenic | 40.0% | |
| ! | AAAGAGGTGCAAAGAGAAGT+GGG | - | Chr1:78904666-78904685 | MsG0180004497.01.T01:CDS | 40.0% |
| ! | CTTTGGGAGAGAATTTCACA+AGG | - | Chr1:78904613-78904632 | MsG0180004497.01.T01:CDS | 40.0% |
| ! | GATACAAGAGAAGTGCAAAG+AGG | - | Chr1:78904650-78904669 | MsG0180004497.01.T01:CDS | 40.0% |
| !! | GAACAAAGAGCTTGAGTTGA+TGG | - | Chr1:78904170-78904189 | MsG0180004497.01.T01:CDS | 40.0% |
| AAGAAGGAAGGGTTGAGCTA+GGG | + | Chr1:78904408-78904427 | None:intergenic | 45.0% | |
| GAAATAGGGTTGTTCTTGCG+AGG | + | Chr1:78904454-78904473 | None:intergenic | 45.0% | |
| GATGAAAACCAAGATGGTGC+TGG | - | Chr1:78904886-78904905 | MsG0180004497.01.T01:CDS | 45.0% | |
| GCACATGAACAAGCTATTGC+TGG | - | Chr1:78904192-78904211 | MsG0180004497.01.T01:CDS | 45.0% | |
| GCACTCACAAAACCCTAATC+CGG | - | Chr1:78904345-78904364 | MsG0180004497.01.T01:CDS | 45.0% | |
| ! | CAAAGAGGTGCAAAGAGAAG+TGG | - | Chr1:78904665-78904684 | MsG0180004497.01.T01:CDS | 45.0% |
| ! | CAACAACAAGGCACCACTTT+GGG | - | Chr1:78904597-78904616 | MsG0180004497.01.T01:CDS | 45.0% |
| AGCAGCGAGAGAGACGATAT+TGG | - | Chr1:78904493-78904512 | MsG0180004497.01.T01:CDS | 50.0% | |
| AGGGTTGAGCTAGGGTTTAC+CGG | + | Chr1:78904400-78904419 | None:intergenic | 50.0% | |
| CAGGTGAGTCTTCTCAAGTG+GGG | - | Chr1:78904911-78904930 | MsG0180004497.01.T01:CDS | 50.0% | |
| CTCTCTCGCTGCTCGATATA+AGG | + | Chr1:78904486-78904505 | None:intergenic | 50.0% | |
| GAAGAAGGAAGGGTTGAGCT+AGG | + | Chr1:78904409-78904428 | None:intergenic | 50.0% | |
| GAGCTAGGGTTTACCGGTAT+TGG | + | Chr1:78904394-78904413 | None:intergenic | 50.0% | |
| GCAGGTGAGTCTTCTCAAGT+GGG | - | Chr1:78904910-78904929 | MsG0180004497.01.T01:CDS | 50.0% | |
| TGCAGGTGAGTCTTCTCAAG+TGG | - | Chr1:78904909-78904928 | MsG0180004497.01.T01:CDS | 50.0% | |
| ! | GCAACAACAAGGCACCACTT+TGG | - | Chr1:78904596-78904615 | MsG0180004497.01.T01:CDS | 50.0% |
| !! | AACCAAGATGGTGCTGGTGC+AGG | - | Chr1:78904892-78904911 | MsG0180004497.01.T01:CDS | 55.0% |
| CACCTGCACCAGCACCATCT+TGG | + | Chr1:78904897-78904916 | None:intergenic | 60.0% | |
| CTCAAGTGGGGCCTCCAAGC+TGG | - | Chr1:78904923-78904942 | MsG0180004497.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 78904057 | 78904948 | 78904057 | ID=MsG0180004497.01;Name=MsG0180004497.01 |
| Chr1 | mRNA | 78904057 | 78904948 | 78904057 | ID=MsG0180004497.01.T01;Parent=MsG0180004497.01;Name=MsG0180004497.01.T01;_AED=0.32;_eAED=0.32;_QI=0|0|0|1|1|1|2|0|268 |
| Chr1 | exon | 78904777 | 78904948 | 78904777 | ID=MsG0180004497.01.T01:exon:13065;Parent=MsG0180004497.01.T01 |
| Chr1 | exon | 78904057 | 78904691 | 78904057 | ID=MsG0180004497.01.T01:exon:13064;Parent=MsG0180004497.01.T01 |
| Chr1 | CDS | 78904777 | 78904948 | 78904777 | ID=MsG0180004497.01.T01:cds;Parent=MsG0180004497.01.T01 |
| Chr1 | CDS | 78904057 | 78904691 | 78904057 | ID=MsG0180004497.01.T01:cds;Parent=MsG0180004497.01.T01 |
| Gene Sequence |
| Protein sequence |
