Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027655.01.T01 | XP_003615100.1 | 93.427 | 213 | 13 | 1 | 1 | 212 | 1 | 213 | 1.94E-135 | 388 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048065.01 | MsG0580027655.01 | 0.807133 | 5.865983e-50 | 3.241332e-47 |
| MsG0080048660.01 | MsG0580027655.01 | 0.800759 | 1.239817e-48 | 5.819903e-46 |
| MsG0080048773.01 | MsG0580027655.01 | 0.848676 | 5.265846e-60 | 9.738829e-57 |
| MsG0080048849.01 | MsG0580027655.01 | 0.821990 | 3.008993e-53 | 2.486430e-50 |
| MsG0080048878.01 | MsG0580027655.01 | 0.825533 | 4.451835e-54 | 4.068708e-51 |
| MsG0080049040.01 | MsG0580027655.01 | 0.814763 | 1.305154e-51 | 8.834233e-49 |
| MsG0080049068.01 | MsG0580027655.01 | 0.816920 | 4.311808e-52 | 3.095862e-49 |
| MsG0180000355.01 | MsG0580027655.01 | 0.809769 | 1.606443e-50 | 9.515711e-48 |
| MsG0180000404.01 | MsG0580027655.01 | 0.820973 | 5.167424e-53 | 4.150361e-50 |
| MsG0180000534.01 | MsG0580027655.01 | 0.804064 | 2.584053e-49 | 1.319682e-46 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027655.01.T01 | MTR_5g063720 | 93.427 | 213 | 13 | 1 | 1 | 212 | 1 | 213 | 2.35e-139 | 388 |
| MsG0580027655.01.T01 | MTR_3g074450 | 53.913 | 230 | 74 | 11 | 1 | 211 | 1 | 217 | 2.10e-59 | 186 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027655.01.T01 | AT1G78170 | 35.217 | 230 | 105 | 9 | 1 | 204 | 1 | 212 | 8.69e-25 | 97.4 |
| MsG0580027655.01.T01 | AT1G22250 | 43.478 | 115 | 51 | 3 | 111 | 211 | 85 | 199 | 3.42e-16 | 74.3 |
| MsG0580027655.01.T01 | AT4G08910 | 74.359 | 39 | 10 | 0 | 158 | 196 | 159 | 197 | 1.05e-15 | 73.2 |
Find 33 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCACTCGTGTTTGAATA+TGG | 0.381186 | 5:+65319776 | MsG0580027655.01.T01:exon |
| AAGAATTCTTGATCAGAAAG+TGG | 0.498028 | 5:-65319891 | None:intergenic |
| AAGGAGTTGTATAATTTGAC+TGG | 0.504963 | 5:+65320774 | MsG0580027655.01.T01:three_prime_UTR |
| AAGGATCTTACACATGATCT+AGG | 0.356603 | 5:+65319915 | MsG0580027655.01.T01:CDS |
| AATTTGCAATTAGGACAAGA+AGG | 0.564845 | 5:-65320560 | None:intergenic |
| AATTTGGAAGGGTTCTGATC+TGG | 0.466878 | 5:-65320596 | None:intergenic |
| ACATAAATTTATGATACACA+AGG | 0.669913 | 5:-65320830 | None:intergenic |
| AGACCATATTCAAACACGAG+TGG | 0.676013 | 5:-65319779 | None:intergenic |
| AGGATCTTACACATGATCTA+GGG | 0.553583 | 5:+65319916 | MsG0580027655.01.T01:CDS |
| ATACAACTCCTTAGAGGTAG+AGG | 0.527574 | 5:-65320763 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGAATTCTTGATCAGAAAG+TGG | - | Chr5:65319894-65319913 | None:intergenic | 30.0% | |
| AAGGAGTTGTATAATTTGAC+TGG | + | Chr5:65320774-65320793 | MsG0580027655.01.T01:three_prime_UTR | 30.0% | |
| AATGTGCTTATAAAAAGGGA+CGG | - | Chr5:65320041-65320060 | None:intergenic | 30.0% | |
| AATTTGCAATTAGGACAAGA+AGG | - | Chr5:65320563-65320582 | None:intergenic | 30.0% | |
| CAAATTATACAACTCCTTAG+AGG | - | Chr5:65320772-65320791 | None:intergenic | 30.0% | |
| CCTTGTTCTCTTCAAAAATT+TGG | - | Chr5:65320615-65320634 | None:intergenic | 30.0% | |
| CTTCACAAAAGAAGAGAAAA+TGG | + | Chr5:65319859-65319878 | MsG0580027655.01.T01:CDS | 30.0% | |
| GCGAAAATGTGCTTATAAAA+AGG | - | Chr5:65320046-65320065 | None:intergenic | 30.0% | |
| GCGAAGTTCTATATCAAATA+TGG | - | Chr5:65319952-65319971 | None:intergenic | 30.0% | |
| GTTCTCTTCAAAAATTTGGA+AGG | - | Chr5:65320611-65320630 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 65319741 | 65320881 | 65319741 | ID=MsG0580027655.01;Name=MsG0580027655.01 |
| Chr5 | mRNA | 65319741 | 65320881 | 65319741 | ID=MsG0580027655.01.T01;Parent=MsG0580027655.01;Name=MsG0580027655.01.T01;_AED=0.18;_eAED=0.18;_QI=54|1|1|1|1|1|2|229|212 |
| Chr5 | exon | 65319741 | 65320022 | 65319741 | ID=MsG0580027655.01.T01:exon:4225;Parent=MsG0580027655.01.T01 |
| Chr5 | exon | 65320242 | 65320881 | 65320242 | ID=MsG0580027655.01.T01:exon:4226;Parent=MsG0580027655.01.T01 |
| Chr5 | five_prime_UTR | 65319741 | 65319794 | 65319741 | ID=MsG0580027655.01.T01:five_prime_utr;Parent=MsG0580027655.01.T01 |
| Chr5 | CDS | 65319795 | 65320022 | 65319795 | ID=MsG0580027655.01.T01:cds;Parent=MsG0580027655.01.T01 |
| Chr5 | CDS | 65320242 | 65320652 | 65320242 | ID=MsG0580027655.01.T01:cds;Parent=MsG0580027655.01.T01 |
| Chr5 | three_prime_UTR | 65320653 | 65320881 | 65320653 | ID=MsG0580027655.01.T01:three_prime_utr;Parent=MsG0580027655.01.T01 |
| Gene Sequence |
>MsG0580027655.01.T01 ATGGTCTCACTTCACAAAGCACTAGCCATGAGTCCAAAAAAAGAATCTCCTACAGATTTTGATGCTTCACAAAAGAAGAGAAAATGGGAAGAATCACCACTTTCTGATCAAGAATTCTTTAAGGATCTTACACATGATCTAGGGAAAAGAAAATCCATATTTGATATAGAACTTCGCCTCAAAACACCTTTTCCTTCAGATAAATGGCAACAATACCTCAATGTTCAGTCAGGGCAAATACAATTGTGTAACACAAAGATGAATAAGAAAACACAAGAATCAAAAAGAGAGTACCCTTTACCTGAAAAATCATCTCTTGGTCATTTAAGCTTAGACCTTGAGCTTAATTTGACATGTGAATCATTGAAGAAAAAAGAAGAAAGTTATTTTGAAAAGAAGAATAATCTAGTGAATGAATCAATTAGCAAGAATAAAAAGGATTCTTCATCATGGTTATCATCATCATCAGATGGTGATGATTACAAAGAAATGATTGCAACAGTTTGTATGAAGTGTCACATGTTGGTAATGCTTTGCAAATCATCTCCTTCTTGTCCTAATTGCAAATTCATGCATCCACCACCAGATCAGAACCCTTCCAAATTTTTGAAGAGAACAAGGTTTAGTTTCTTGACATAA |
| Protein sequence |
>MsG0580027655.01.T01 MVSLHKALAMSPKKESPTDFDASQKKRKWEESPLSDQEFFKDLTHDLGKRKSIFDIELRLKTPFPSDKWQQYLNVQSGQIQLCNTKMNKKTQESKREYPLPEKSSLGHLSLDLELNLTCESLKKKEESYFEKKNNLVNESISKNKKDSSSWLSSSSDGDDYKEMIATVCMKCHMLVMLCKSSPSCPNCKFMHPPPDQNPSKFLKRTRFSFLT* |
