AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580027655.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027655.01.T01 MTR_5g063720 93.427 213 13 1 1 212 1 213 2.35e-139 388
MsG0580027655.01.T01 MTR_3g074450 53.913 230 74 11 1 211 1 217 2.10e-59 186
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027655.01.T01 AT1G78170 35.217 230 105 9 1 204 1 212 8.69e-25 97.4
MsG0580027655.01.T01 AT1G22250 43.478 115 51 3 111 211 85 199 3.42e-16 74.3
MsG0580027655.01.T01 AT4G08910 74.359 39 10 0 158 196 159 197 1.05e-15 73.2

Find 33 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGATCAAGAATTCTTTA+AGG 0.147055 5:+65319896 MsG0580027655.01.T01:CDS
GATTACATAGACTTATTTAT+TGG 0.200096 5:-65320678 None:intergenic
CTTCACAAAAGAAGAGAAAA+TGG 0.266395 5:+65319859 MsG0580027655.01.T01:CDS
GCGAAGTTCTATATCAAATA+TGG 0.295622 5:-65319949 None:intergenic
ATCAATTAGCAAGAATAAAA+AGG 0.319819 5:+65320430 MsG0580027655.01.T01:CDS
TATTATATATTTCAAACAAA+AGG 0.340498 5:+65320725 MsG0580027655.01.T01:three_prime_UTR
CAAGAACAACCACTTCTAAT+TGG 0.345178 5:+65320654 MsG0580027655.01.T01:three_prime_UTR
AAGGATCTTACACATGATCT+AGG 0.356603 5:+65319915 MsG0580027655.01.T01:CDS
GGATGCATGAATTTGCAATT+AGG 0.374086 5:-65320569 None:intergenic
AAACCACTCGTGTTTGAATA+TGG 0.381186 5:+65319776 MsG0580027655.01.T01:exon
CAAATTATACAACTCCTTAG+AGG 0.387146 5:-65320769 None:intergenic
GCAACAATACCTCAATGTTC+AGG 0.426385 5:+65320001 MsG0580027655.01.T01:CDS
TTAATCTACCTCTACCTCTA+AGG 0.434008 5:+65320755 MsG0580027655.01.T01:three_prime_UTR
ATTATATATTTCAAACAAAA+GGG 0.459409 5:+65320726 MsG0580027655.01.T01:three_prime_UTR
AATTTGGAAGGGTTCTGATC+TGG 0.466878 5:-65320596 None:intergenic
GAAGGGTTCTGATCTGGTGG+TGG 0.491999 5:-65320590 None:intergenic
TGCCATTTATCTGAAGGAAA+AGG 0.492962 5:-65319981 None:intergenic
AAGAATTCTTGATCAGAAAG+TGG 0.498028 5:-65319891 None:intergenic
AAGGAGTTGTATAATTTGAC+TGG 0.504963 5:+65320774 MsG0580027655.01.T01:three_prime_UTR
TTCACAAAAGAAGAGAAAAT+GGG 0.522716 5:+65319860 MsG0580027655.01.T01:CDS
ATACAACTCCTTAGAGGTAG+AGG 0.527574 5:-65320763 None:intergenic
TATTGTTGCCATTTATCTGA+AGG 0.533510 5:-65319987 None:intergenic
AGGATCTTACACATGATCTA+GGG 0.553583 5:+65319916 MsG0580027655.01.T01:CDS
TGTGAAGCATCAAAATCTGT+AGG 0.557840 5:-65319843 None:intergenic
CACATGTCAAATTAAGCTCA+AGG 0.558418 5:-65320349 None:intergenic
AATTTGCAATTAGGACAAGA+AGG 0.564845 5:-65320560 None:intergenic
TTTATTGGACCAATTAGAAG+TGG 0.571145 5:-65320663 None:intergenic
TTGGAAGGGTTCTGATCTGG+TGG 0.597114 5:-65320593 None:intergenic
TGGTTATCATCATCATCAGA+TGG 0.614533 5:+65320464 MsG0580027655.01.T01:CDS
TATTATATACCTGAACATTG+AGG 0.617071 5:-65320010 None:intergenic
ACATAAATTTATGATACACA+AGG 0.669913 5:-65320830 None:intergenic
AGACCATATTCAAACACGAG+TGG 0.676013 5:-65319779 None:intergenic
TTGTATGAAGTGTCACATGT+TGG 0.683627 5:+65320517 MsG0580027655.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATATATTTCAAACAAAA+GGG + Chr5:65320726-65320745 MsG0580027655.01.T01:three_prime_UTR 10.0%
!! TATTATATATTTCAAACAAA+AGG + Chr5:65320725-65320744 MsG0580027655.01.T01:three_prime_UTR 10.0%
!! AAAATGAAATATATTAACCT+CGG - Chr5:65320089-65320108 None:intergenic 15.0%
!! ACATAAATTTATGATACACA+AGG - Chr5:65320833-65320852 None:intergenic 20.0%
!! ATAAAATATTATTGCTACCT+CGG - Chr5:65320135-65320154 None:intergenic 20.0%
!! ATCAATTAGCAAGAATAAAA+AGG + Chr5:65320430-65320449 MsG0580027655.01.T01:CDS 20.0%
!! GATTACATAGACTTATTTAT+TGG - Chr5:65320681-65320700 None:intergenic 20.0%
! ATAAAAAGGATTCTTCATCA+TGG + Chr5:65320444-65320463 MsG0580027655.01.T01:CDS 25.0%
! CGAAAATGTGCTTATAAAAA+GGG - Chr5:65320045-65320064 None:intergenic 25.0%
! TATTATATACCTGAACATTG+AGG - Chr5:65320013-65320032 None:intergenic 25.0%
! TTCAAAATAAATTAGAACCG+AGG + Chr5:65320115-65320134 MsG0580027655.01.T01:intron 25.0%
! TTCACAAAAGAAGAGAAAAT+GGG + Chr5:65319860-65319879 MsG0580027655.01.T01:CDS 25.0%
! TTCTCTTCAAAAATTTGGAA+GGG - Chr5:65320610-65320629 None:intergenic 25.0%
! TTCTGATCAAGAATTCTTTA+AGG + Chr5:65319896-65319915 MsG0580027655.01.T01:CDS 25.0%
AAGAATTCTTGATCAGAAAG+TGG - Chr5:65319894-65319913 None:intergenic 30.0%
AAGGAGTTGTATAATTTGAC+TGG + Chr5:65320774-65320793 MsG0580027655.01.T01:three_prime_UTR 30.0%
AATGTGCTTATAAAAAGGGA+CGG - Chr5:65320041-65320060 None:intergenic 30.0%
AATTTGCAATTAGGACAAGA+AGG - Chr5:65320563-65320582 None:intergenic 30.0%
CAAATTATACAACTCCTTAG+AGG - Chr5:65320772-65320791 None:intergenic 30.0%
CCTTGTTCTCTTCAAAAATT+TGG - Chr5:65320615-65320634 None:intergenic 30.0%
CTTCACAAAAGAAGAGAAAA+TGG + Chr5:65319859-65319878 MsG0580027655.01.T01:CDS 30.0%
GCGAAAATGTGCTTATAAAA+AGG - Chr5:65320046-65320065 None:intergenic 30.0%
GCGAAGTTCTATATCAAATA+TGG - Chr5:65319952-65319971 None:intergenic 30.0%
GTTCTCTTCAAAAATTTGGA+AGG - Chr5:65320611-65320630 None:intergenic 30.0%
TTACCTGAAAAATCATCTCT+TGG + Chr5:65320311-65320330 MsG0580027655.01.T01:CDS 30.0%
TTTATTGGACCAATTAGAAG+TGG - Chr5:65320666-65320685 None:intergenic 30.0%
! TAAATTTTCTCATGCAGTCA+GGG + Chr5:65320225-65320244 MsG0580027655.01.T01:intron 30.0%
! TATTGTTGCCATTTATCTGA+AGG - Chr5:65319990-65320009 None:intergenic 30.0%
! TTAAATTTTCTCATGCAGTC+AGG + Chr5:65320224-65320243 MsG0580027655.01.T01:intron 30.0%
!! CCAAATTTTTGAAGAGAACA+AGG + Chr5:65320612-65320631 MsG0580027655.01.T01:CDS 30.0%
!!! AGAGATGATTTTTCAGGTAA+AGG - Chr5:65320311-65320330 None:intergenic 30.0%
!!! AGATTCTTTTTTTGGACTCA+TGG - Chr5:65319823-65319842 None:intergenic 30.0%
!!! GAGATGATTTTTCAGGTAAA+GGG - Chr5:65320310-65320329 None:intergenic 30.0%
!!! TCTGTAGGAGATTCTTTTTT+TGG - Chr5:65319831-65319850 None:intergenic 30.0%
AAACCACTCGTGTTTGAATA+TGG + Chr5:65319776-65319795 MsG0580027655.01.T01:exon 35.0%
AAGGATCTTACACATGATCT+AGG + Chr5:65319915-65319934 MsG0580027655.01.T01:CDS 35.0%
AGGATCTTACACATGATCTA+GGG + Chr5:65319916-65319935 MsG0580027655.01.T01:CDS 35.0%
CAAGAACAACCACTTCTAAT+TGG + Chr5:65320654-65320673 MsG0580027655.01.T01:three_prime_UTR 35.0%
CACATGTCAAATTAAGCTCA+AGG - Chr5:65320352-65320371 None:intergenic 35.0%
GGATGCATGAATTTGCAATT+AGG - Chr5:65320572-65320591 None:intergenic 35.0%
TGGTTATCATCATCATCAGA+TGG + Chr5:65320464-65320483 MsG0580027655.01.T01:CDS 35.0%
TTAATCTACCTCTACCTCTA+AGG + Chr5:65320755-65320774 MsG0580027655.01.T01:three_prime_UTR 35.0%
! CACCTTTTCCTTCAGATAAA+TGG + Chr5:65319979-65319998 MsG0580027655.01.T01:CDS 35.0%
! TGACCAAGAGATGATTTTTC+AGG - Chr5:65320317-65320336 None:intergenic 35.0%
! TGCCATTTATCTGAAGGAAA+AGG - Chr5:65319984-65320003 None:intergenic 35.0%
! TGTGAAGCATCAAAATCTGT+AGG - Chr5:65319846-65319865 None:intergenic 35.0%
! TTGTATGAAGTGTCACATGT+TGG + Chr5:65320517-65320536 MsG0580027655.01.T01:CDS 35.0%
!! CTAGTTTTTAATTAGAGCCG+AGG + Chr5:65320069-65320088 MsG0580027655.01.T01:intron 35.0%
AGACCATATTCAAACACGAG+TGG - Chr5:65319782-65319801 None:intergenic 40.0%
ATACAACTCCTTAGAGGTAG+AGG - Chr5:65320766-65320785 None:intergenic 40.0%
GCAACAATACCTCAATGTTC+AGG + Chr5:65320001-65320020 MsG0580027655.01.T01:CDS 40.0%
! AATTTGGAAGGGTTCTGATC+TGG - Chr5:65320599-65320618 None:intergenic 40.0%
!!! CTGAAGGAAAAGGTGTTTTG+AGG - Chr5:65319974-65319993 None:intergenic 40.0%
TTCTCTCCAGTAGTTGTGAG+CGG - Chr5:65319742-65319761 None:intergenic 45.0%
! TTGGAAGGGTTCTGATCTGG+TGG - Chr5:65320596-65320615 None:intergenic 50.0%
! GAAGGGTTCTGATCTGGTGG+TGG - Chr5:65320593-65320612 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 65319741 65320881 65319741 ID=MsG0580027655.01;Name=MsG0580027655.01
Chr5 mRNA 65319741 65320881 65319741 ID=MsG0580027655.01.T01;Parent=MsG0580027655.01;Name=MsG0580027655.01.T01;_AED=0.18;_eAED=0.18;_QI=54|1|1|1|1|1|2|229|212
Chr5 exon 65319741 65320022 65319741 ID=MsG0580027655.01.T01:exon:4225;Parent=MsG0580027655.01.T01
Chr5 exon 65320242 65320881 65320242 ID=MsG0580027655.01.T01:exon:4226;Parent=MsG0580027655.01.T01
Chr5 five_prime_UTR 65319741 65319794 65319741 ID=MsG0580027655.01.T01:five_prime_utr;Parent=MsG0580027655.01.T01
Chr5 CDS 65319795 65320022 65319795 ID=MsG0580027655.01.T01:cds;Parent=MsG0580027655.01.T01
Chr5 CDS 65320242 65320652 65320242 ID=MsG0580027655.01.T01:cds;Parent=MsG0580027655.01.T01
Chr5 three_prime_UTR 65320653 65320881 65320653 ID=MsG0580027655.01.T01:three_prime_utr;Parent=MsG0580027655.01.T01
Gene Sequence

>MsG0580027655.01.T01

ATGGTCTCACTTCACAAAGCACTAGCCATGAGTCCAAAAAAAGAATCTCCTACAGATTTTGATGCTTCACAAAAGAAGAGAAAATGGGAAGAATCACCACTTTCTGATCAAGAATTCTTTAAGGATCTTACACATGATCTAGGGAAAAGAAAATCCATATTTGATATAGAACTTCGCCTCAAAACACCTTTTCCTTCAGATAAATGGCAACAATACCTCAATGTTCAGTCAGGGCAAATACAATTGTGTAACACAAAGATGAATAAGAAAACACAAGAATCAAAAAGAGAGTACCCTTTACCTGAAAAATCATCTCTTGGTCATTTAAGCTTAGACCTTGAGCTTAATTTGACATGTGAATCATTGAAGAAAAAAGAAGAAAGTTATTTTGAAAAGAAGAATAATCTAGTGAATGAATCAATTAGCAAGAATAAAAAGGATTCTTCATCATGGTTATCATCATCATCAGATGGTGATGATTACAAAGAAATGATTGCAACAGTTTGTATGAAGTGTCACATGTTGGTAATGCTTTGCAAATCATCTCCTTCTTGTCCTAATTGCAAATTCATGCATCCACCACCAGATCAGAACCCTTCCAAATTTTTGAAGAGAACAAGGTTTAGTTTCTTGACATAA

Protein sequence

>MsG0580027655.01.T01

MVSLHKALAMSPKKESPTDFDASQKKRKWEESPLSDQEFFKDLTHDLGKRKSIFDIELRLKTPFPSDKWQQYLNVQSGQIQLCNTKMNKKTQESKREYPLPEKSSLGHLSLDLELNLTCESLKKKEESYFEKKNNLVNESISKNKKDSSSWLSSSSDGDDYKEMIATVCMKCHMLVMLCKSSPSCPNCKFMHPPPDQNPSKFLKRTRFSFLT*