AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004046.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004046.01.T01 MTR_1g073620 100.000 126 0 0 1 126 1 126 4.15e-90 256
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004046.01.T01 AT5G64816 83.333 120 20 0 1 120 1 120 1.50e-74 218
MsG0180004046.01.T01 AT5G64816 83.333 120 20 0 1 120 1 120 1.50e-74 218
MsG0180004046.01.T01 AT5G64816 83.333 120 20 0 1 120 1 120 1.50e-74 218
MsG0180004046.01.T01 AT5G64816 83.333 120 20 0 1 120 1 120 1.50e-74 218

Find 24 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATATTTGGTGGTCACTTT+TGG 0.138181 1:-72465243 MsG0180004046.01.T01:CDS
TTCAAAGAGGATGTTGAAAA+AGG 0.272946 1:-72465084 MsG0180004046.01.T01:CDS
ACCAACATCAAGTACCTAAT+TGG 0.307555 1:-72464956 MsG0180004046.01.T01:CDS
GATACTTGTGTTACTGTTTG+TGG 0.347747 1:-72465032 MsG0180004046.01.T01:CDS
GCAGTCCCTGTCGTTGTTGC+AGG 0.348680 1:-72465215 MsG0180004046.01.T01:CDS
TATCAGAGATGGGAGATATT+TGG 0.353189 1:-72465256 None:intergenic
ATTGGAATGACACATGTCTT+AGG 0.381625 1:-72464938 MsG0180004046.01.T01:CDS
AGACATGTGTCATTCCAATT+AGG 0.386451 1:+72464942 None:intergenic
GTAACACAAGTATCAGGAAT+TGG 0.406394 1:+72465041 None:intergenic
CAAACAGTAACACAAGTATC+AGG 0.430522 1:+72465035 None:intergenic
GCTTGTCCTGCAACAACGAC+AGG 0.451940 1:+72465209 None:intergenic
TCCAATTAGGTACTTGATGT+TGG 0.474351 1:+72464955 None:intergenic
AAGCATTTAGAGTTAAGAAA+AGG 0.483524 1:-72465190 MsG0180004046.01.T01:CDS
TGCTGAAGCTTGTGCTCGTA+CGG 0.484272 1:-72464988 MsG0180004046.01.T01:CDS
AGCAAAGGTTGAAGAGTGCA+AGG 0.518677 1:-72465157 MsG0180004046.01.T01:CDS
AAAGGAATGCAGAGGAGCAA+AGG 0.541153 1:-72465172 MsG0180004046.01.T01:CDS
AGAGTGCAAGGGGAAGAGAA+AGG 0.552353 1:-72465145 MsG0180004046.01.T01:CDS
CAGAGATGGGAGATATTTGG+TGG 0.589138 1:-72465253 None:intergenic
CAAAGGTTGAAGAGTGCAAG+GGG 0.608201 1:-72465155 MsG0180004046.01.T01:CDS
GCAAAGGTTGAAGAGTGCAA+GGG 0.610878 1:-72465156 MsG0180004046.01.T01:CDS
AGTTAAGAAAAGGAATGCAG+AGG 0.619043 1:-72465180 MsG0180004046.01.T01:CDS
CTTGTCCTGCAACAACGACA+GGG 0.623317 1:+72465210 None:intergenic
GGAATGACACATGTCTTAGG+AGG 0.635618 1:-72464935 MsG0180004046.01.T01:CDS
AAAGGGTCTGCACTTCAAAG+AGG 0.655293 1:-72465097 MsG0180004046.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGCATTTAGAGTTAAGAAA+AGG - Chr1:72464948-72464967 MsG0180004046.01.T01:CDS 25.0%
!! ATGAAATTTTCGTTTGTGAA+AGG - Chr1:72465023-72465042 MsG0180004046.01.T01:CDS 25.0%
!! TGAAATTTTCGTTTGTGAAA+GGG - Chr1:72465024-72465043 MsG0180004046.01.T01:CDS 25.0%
TTCAAAGAGGATGTTGAAAA+AGG - Chr1:72465054-72465073 MsG0180004046.01.T01:CDS 30.0%
AAGAGGATGTTGAAAAAGGT+TGG - Chr1:72465058-72465077 MsG0180004046.01.T01:CDS 35.0%
ACCAACATCAAGTACCTAAT+TGG - Chr1:72465182-72465201 MsG0180004046.01.T01:CDS 35.0%
AGTTAAGAAAAGGAATGCAG+AGG - Chr1:72464958-72464977 MsG0180004046.01.T01:CDS 35.0%
ATTGGAATGACACATGTCTT+AGG - Chr1:72465200-72465219 MsG0180004046.01.T01:CDS 35.0%
CAAACAGTAACACAAGTATC+AGG + Chr1:72465106-72465125 None:intergenic 35.0%
GTAACACAAGTATCAGGAAT+TGG + Chr1:72465100-72465119 None:intergenic 35.0%
TCCAATTAGGTACTTGATGT+TGG + Chr1:72465186-72465205 None:intergenic 35.0%
! AGACATGTGTCATTCCAATT+AGG + Chr1:72465199-72465218 None:intergenic 35.0%
! GATACTTGTGTTACTGTTTG+TGG - Chr1:72465106-72465125 MsG0180004046.01.T01:CDS 35.0%
!! AGATATTTGGTGGTCACTTT+TGG - Chr1:72464895-72464914 MsG0180004046.01.T01:CDS 35.0%
!!! ATATTTGGTGGTCACTTTTG+GGG - Chr1:72464897-72464916 MsG0180004046.01.T01:CDS 35.0%
!!! GATATTTGGTGGTCACTTTT+GGG - Chr1:72464896-72464915 MsG0180004046.01.T01:CDS 35.0%
!!! TATTTGGTGGTCACTTTTGG+GGG - Chr1:72464898-72464917 MsG0180004046.01.T01:CDS 40.0%
AAAGGAATGCAGAGGAGCAA+AGG - Chr1:72464966-72464985 MsG0180004046.01.T01:CDS 45.0%
AAAGGGTCTGCACTTCAAAG+AGG - Chr1:72465041-72465060 MsG0180004046.01.T01:CDS 45.0%
GGAATGACACATGTCTTAGG+AGG - Chr1:72465203-72465222 MsG0180004046.01.T01:CDS 45.0%
! AGCAAAGGTTGAAGAGTGCA+AGG - Chr1:72464981-72465000 MsG0180004046.01.T01:CDS 45.0%
! CAAAGGTTGAAGAGTGCAAG+GGG - Chr1:72464983-72465002 MsG0180004046.01.T01:CDS 45.0%
! GCAAAGGTTGAAGAGTGCAA+GGG - Chr1:72464982-72465001 MsG0180004046.01.T01:CDS 45.0%
AGAGTGCAAGGGGAAGAGAA+AGG - Chr1:72464993-72465012 MsG0180004046.01.T01:CDS 50.0%
CTTGTCCTGCAACAACGACA+GGG + Chr1:72464931-72464950 None:intergenic 50.0%
TGCTGAAGCTTGTGCTCGTA+CGG - Chr1:72465150-72465169 MsG0180004046.01.T01:CDS 50.0%
GCTTGTCCTGCAACAACGAC+AGG + Chr1:72464932-72464951 None:intergenic 55.0%
GCAGTCCCTGTCGTTGTTGC+AGG - Chr1:72464923-72464942 MsG0180004046.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 72464890 72465270 72464890 ID=MsG0180004046.01;Name=MsG0180004046.01
Chr1 mRNA 72464890 72465270 72464890 ID=MsG0180004046.01.T01;Parent=MsG0180004046.01;Name=MsG0180004046.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|126
Chr1 exon 72464890 72465270 72464890 ID=MsG0180004046.01.T01:exon:5061;Parent=MsG0180004046.01.T01
Chr1 CDS 72464890 72465270 72464890 ID=MsG0180004046.01.T01:cds;Parent=MsG0180004046.01.T01
Gene Sequence

>MsG0180004046.01.T01

ATGGGAGATATTTGGTGGTCACTTTTGGGGGCAGCAGTCCCTGTCGTTGTTGCAGGACAAGCATTTAGAGTTAAGAAAAGGAATGCAGAGGAGCAAAGGTTGAAGAGTGCAAGGGGAAGAGAAAGGAGTTCTGATGAAATTTTCGTTTGTGAAAGGGTCTGCACTTCAAAGAGGATGTTGAAAAAGGTTGGCTCTTTCTCAAAAGATCCAATTCCTGATACTTGTGTTACTGTTTGTGGTGTGTCTGATCTTGACGCGTGTGCTGAAGCTTGTGCTCGTACGGTTTGTGTTAACCAACATCAAGTACCTAATTGGAATGACACATGTCTTAGGAGGTGTCAGAGTGAATGCTTGAAACTCTCTTCTCAGTCTTCTCAGTAG

Protein sequence

>MsG0180004046.01.T01

MGDIWWSLLGAAVPVVVAGQAFRVKKRNAEEQRLKSARGRERSSDEIFVCERVCTSKRMLKKVGSFSKDPIPDTCVTVCGVSDLDACAEACARTVCVNQHQVPNWNDTCLRRCQSECLKLSSQSSQ*