AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038847.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038847.01.T01 MTR_7g063360 91.071 56 5 0 12 67 211 266 6.44e-30 105
MsG0780038847.01.T01 MTR_7g063390 77.193 57 13 0 11 67 210 266 2.76e-25 94.0
MsG0780038847.01.T01 MTR_7g063270 71.429 49 14 0 18 66 167 215 2.64e-19 77.4
MsG0780038847.01.T01 MTR_7g063320 72.340 47 13 0 21 67 219 265 5.13e-19 77.4
MsG0780038847.01.T01 MTR_7g063270 71.429 49 14 0 18 66 216 264 7.09e-19 77.0
MsG0780038847.01.T01 MTR_7g063260 73.913 46 12 0 21 66 220 265 8.43e-18 74.3
MsG0780038847.01.T01 MTR_7g063330 72.340 47 13 0 20 66 217 263 3.61e-17 72.8
MsG0780038847.01.T01 MTR_3g068155 59.574 47 19 0 21 67 219 265 5.61e-15 67.0
MsG0780038847.01.T01 MTR_4g064887 56.000 50 22 0 18 67 212 261 1.82e-14 65.5
MsG0780038847.01.T01 MTR_3g068065 60.870 46 18 0 21 66 217 262 3.33e-14 64.7
MsG0780038847.01.T01 MTR_3g068070 57.447 47 20 0 21 67 215 261 5.59e-14 64.3
MsG0780038847.01.T01 MTR_3g068085 54.000 50 23 0 18 67 212 261 8.99e-14 63.5
MsG0780038847.01.T01 MTR_4g064883 55.102 49 22 0 19 67 212 260 1.02e-13 63.5
MsG0780038847.01.T01 MTR_3g068175 57.447 47 20 0 21 67 217 263 1.61e-13 62.8
MsG0780038847.01.T01 MTR_3g068175 57.447 47 20 0 21 67 221 267 1.66e-13 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038847.01.T01 AT5G06060 65.957 47 16 0 21 67 209 255 4.96e-18 75.1
MsG0780038847.01.T01 AT2G29260 48.485 66 27 2 8 67 250 314 1.48e-15 68.9
MsG0780038847.01.T01 AT2G29320 60.870 46 18 0 22 67 197 242 4.54e-15 67.0
MsG0780038847.01.T01 AT2G29310 60.870 46 18 0 22 67 206 251 9.34e-15 66.2
MsG0780038847.01.T01 AT2G29320 60.870 46 18 0 22 67 215 260 1.16e-14 65.9
MsG0780038847.01.T01 AT2G30670 63.043 46 17 0 22 67 208 253 1.56e-14 65.5
MsG0780038847.01.T01 AT2G29370 50.769 65 27 1 8 67 198 262 2.64e-14 65.1
MsG0780038847.01.T01 AT2G29340 61.364 44 17 0 24 67 210 253 2.95e-14 64.7
MsG0780038847.01.T01 AT2G29340 61.364 44 17 0 24 67 210 253 4.53e-14 64.7
MsG0780038847.01.T01 AT2G29310 60.870 46 18 0 22 67 208 253 5.70e-14 63.9
MsG0780038847.01.T01 AT2G29330 60.870 46 18 0 22 67 208 253 1.16e-13 63.2
MsG0780038847.01.T01 AT1G07450 60.870 46 18 0 22 67 208 253 1.22e-13 63.2
MsG0780038847.01.T01 AT2G29290 65.000 40 14 0 28 67 197 236 1.58e-13 62.8
MsG0780038847.01.T01 AT1G07440 59.091 44 18 0 24 67 214 257 2.10e-13 62.8
MsG0780038847.01.T01 AT2G29300 60.000 45 18 0 22 66 209 253 2.42e-13 62.8
MsG0780038847.01.T01 AT2G29290 65.000 40 14 0 28 67 214 253 2.82e-13 62.4
MsG0780038847.01.T01 AT2G29360 58.696 46 19 0 22 67 217 262 2.89e-13 62.4
MsG0780038847.01.T01 AT2G29300 58.696 46 19 0 22 67 209 254 3.42e-13 62.0
MsG0780038847.01.T01 AT2G29350 56.522 46 20 0 22 67 177 222 7.83e-13 60.8
MsG0780038847.01.T01 AT2G29350 56.522 46 20 0 22 67 215 260 2.30e-12 59.7
MsG0780038847.01.T01 AT2G29150 60.000 40 16 0 28 67 222 261 8.79e-12 58.2

Find 20 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTGTTATAGAAATTAAC+TGG 0.353373 7:+53968947 MsG0780038847.01.T01:intron
GGATAATGTTGTCGAGGATA+TGG 0.392949 7:+53968976 MsG0780038847.01.T01:CDS
GATACAGTGGCGGATCTCCT+TGG 0.491297 7:-53968188 None:intergenic
GCCAATGCTGATATATCCTT+AGG 0.509059 7:-53969031 None:intergenic
ACCTAAGGATATATCAGCAT+TGG 0.530498 7:+53969030 MsG0780038847.01.T01:CDS
TGGTCGGATTGGAGAACCTA+AGG 0.554317 7:+53969015 MsG0780038847.01.T01:CDS
CTAACACCTAGTGGTCGGAT+TGG 0.555165 7:+53969004 MsG0780038847.01.T01:CDS
CCAGTGATGTGTGATGCAGC+TGG 0.556867 7:-53969070 None:intergenic
GGTTCTCCAATCCGACCACT+AGG 0.556909 7:-53969010 None:intergenic
CCAGCTGCATCACACATCAC+TGG 0.560488 7:+53969070 MsG0780038847.01.T01:CDS
GGATCTCCTTGGAGACCCAT+AGG 0.574980 7:-53968177 None:intergenic
CAAATTATAGCTGCAGATGG+AGG 0.585129 7:+53969094 MsG0780038847.01.T01:CDS
GGACAAATTATAGCTGCAGA+TGG 0.588163 7:+53969091 MsG0780038847.01.T01:CDS
CACAATGGATAATGTTGTCG+AGG 0.608245 7:+53968970 MsG0780038847.01.T01:CDS
GTGGCACCTATGGGTCTCCA+AGG 0.609564 7:+53968171 None:intergenic
ATAGAAATTAACTGGCACAA+TGG 0.645596 7:+53968955 MsG0780038847.01.T01:intron
TGTCTCTAACACCTAGTGGT+CGG 0.659290 7:+53968999 MsG0780038847.01.T01:CDS
TGAGATCTCACGAGATACAG+TGG 0.666039 7:-53968201 None:intergenic
ATGGTGTCTCTAACACCTAG+TGG 0.670463 7:+53968995 MsG0780038847.01.T01:CDS
GATCTCACGAGATACAGTGG+CGG 0.768719 7:-53968198 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAAAATAAAAACATCT+TGG + Chr7:53968764-53968783 MsG0780038847.01.T01:intron 10.0%
!! TAACAACTTAATAACATTAA+GGG - Chr7:53968462-53968481 None:intergenic 15.0%
!! TATACATACAAGTTTAAATT+AGG - Chr7:53968895-53968914 None:intergenic 15.0%
!! TTAACAACTTAATAACATTA+AGG - Chr7:53968463-53968482 None:intergenic 15.0%
!! AACAACTTAATAACATTAAG+GGG - Chr7:53968461-53968480 None:intergenic 20.0%
!! ACAAGAGATTATAAACATTA+TGG - Chr7:53968934-53968953 None:intergenic 20.0%
!! ACTATAAAAGGATAATTGAA+AGG - Chr7:53968333-53968352 None:intergenic 20.0%
!!! GTTATTAAGTTGTTAACTAT+GGG + Chr7:53968467-53968486 MsG0780038847.01.T01:intron 20.0%
!!! TGTTATTAAGTTGTTAACTA+TGG + Chr7:53968466-53968485 MsG0780038847.01.T01:intron 20.0%
! AAAGTTTAATATCAAGCAGA+AGG - Chr7:53968397-53968416 None:intergenic 25.0%
! AAGTTTAATATCAAGCAGAA+GGG - Chr7:53968396-53968415 None:intergenic 25.0%
! ACAACTTAATAACATTAAGG+GGG - Chr7:53968460-53968479 None:intergenic 25.0%
! ACTATGATACTTAATGTAGA+AGG + Chr7:53968245-53968264 MsG0780038847.01.T01:intron 25.0%
! AGAAAAACTTGAAGCTAAAT+TGG + Chr7:53968811-53968830 MsG0780038847.01.T01:intron 25.0%
! TATTCTGACACTACTATAAA+AGG - Chr7:53968345-53968364 None:intergenic 25.0%
! TGCTTGATATTAAACTTTGT+TGG + Chr7:53968399-53968418 MsG0780038847.01.T01:intron 25.0%
!! CTCTTGTTATAGAAATTAAC+TGG + Chr7:53968947-53968966 MsG0780038847.01.T01:intron 25.0%
AGAATATCTAATGTGCTTCA+TGG + Chr7:53968359-53968378 MsG0780038847.01.T01:intron 30.0%
ATAATTGAAAGGATCCTTCA+TGG - Chr7:53968322-53968341 None:intergenic 30.0%
ATAGAAATTAACTGGCACAA+TGG + Chr7:53968955-53968974 MsG0780038847.01.T01:intron 30.0%
TTCTCAAACTCAAGATAGAT+TGG + Chr7:53968856-53968875 MsG0780038847.01.T01:intron 30.0%
! TTATGTCATACTCTTTGAGA+AGG + Chr7:53968552-53968571 MsG0780038847.01.T01:intron 30.0%
AATGCGCCAATATTTCACAA+TGG - Chr7:53968530-53968549 None:intergenic 35.0%
ACCTAAGGATATATCAGCAT+TGG + Chr7:53969030-53969049 MsG0780038847.01.T01:CDS 35.0%
AGCATATGCTAATAGAGCAT+AGG - Chr7:53968664-53968683 None:intergenic 35.0%
ATGCTAATAGAGCATAGGAA+AGG - Chr7:53968659-53968678 None:intergenic 35.0%
CTAGCTAAACTGAAAGCTAA+CGG + Chr7:53968594-53968613 MsG0780038847.01.T01:intron 35.0%
GTAGGACCATTGTGAAATAT+TGG + Chr7:53968521-53968540 MsG0780038847.01.T01:intron 35.0%
TAAATTGGCACCAGTCAAAA+CGG + Chr7:53968826-53968845 MsG0780038847.01.T01:intron 35.0%
TATTTCACAATGGTCCTACA+AGG - Chr7:53968520-53968539 None:intergenic 35.0%
! ACTAGACTAGTTCCTTATGA+AGG - Chr7:53968697-53968716 None:intergenic 35.0%
! AGCATAGGAAAGGCATAAAA+GGG - Chr7:53968649-53968668 None:intergenic 35.0%
!! GAATGAAAATCCGTTTTGAC+TGG - Chr7:53968839-53968858 None:intergenic 35.0%
CAAATTATAGCTGCAGATGG+AGG + Chr7:53969094-53969113 MsG0780038847.01.T01:CDS 40.0%
CACAATGGATAATGTTGTCG+AGG + Chr7:53968970-53968989 MsG0780038847.01.T01:CDS 40.0%
GCCAATGCTGATATATCCTT+AGG - Chr7:53969034-53969053 None:intergenic 40.0%
GCTCTATTAGCATATGCTCT+AGG + Chr7:53968666-53968685 MsG0780038847.01.T01:intron 40.0%
GGACAAATTATAGCTGCAGA+TGG + Chr7:53969091-53969110 MsG0780038847.01.T01:CDS 40.0%
GGATAATGTTGTCGAGGATA+TGG + Chr7:53968976-53968995 MsG0780038847.01.T01:CDS 40.0%
GTCCTACAAGGTTAGCATAA+GGG - Chr7:53968508-53968527 None:intergenic 40.0%
! GAGCATAGGAAAGGCATAAA+AGG - Chr7:53968650-53968669 None:intergenic 40.0%
! GCATAGGAAAGGCATAAAAG+GGG - Chr7:53968648-53968667 None:intergenic 40.0%
AAAAGCCGAGTTTGGAGGTT+TGG + Chr7:53968289-53968308 MsG0780038847.01.T01:intron 45.0%
AACCCAAGAAAAGCCGAGTT+TGG + Chr7:53968281-53968300 MsG0780038847.01.T01:intron 45.0%
ATGGTGTCTCTAACACCTAG+TGG + Chr7:53968995-53969014 MsG0780038847.01.T01:CDS 45.0%
CACCCTTATGCTAACCTTGT+AGG + Chr7:53968503-53968522 MsG0780038847.01.T01:intron 45.0%
CCTTACCAAACCTCCAAACT+CGG - Chr7:53968297-53968316 None:intergenic 45.0%
CTACAAGGTTAGCATAAGGG+TGG - Chr7:53968505-53968524 None:intergenic 45.0%
CTGTATCTCGTGAGATCTCA+TGG + Chr7:53968204-53968223 MsG0780038847.01.T01:intron 45.0%
GGTCCTACAAGGTTAGCATA+AGG - Chr7:53968509-53968528 None:intergenic 45.0%
TGAGATCTCACGAGATACAG+TGG - Chr7:53968204-53968223 None:intergenic 45.0%
TGTCTCTAACACCTAGTGGT+CGG + Chr7:53968999-53969018 MsG0780038847.01.T01:CDS 45.0%
! CTCCAAACTCGGCTTTTCTT+GGG - Chr7:53968286-53968305 None:intergenic 45.0%
! CTCTAGGAGTCTCCTTCATA+AGG + Chr7:53968682-53968701 MsG0780038847.01.T01:intron 45.0%
! GGTTTGGTAAGGAACCATGA+AGG + Chr7:53968305-53968324 MsG0780038847.01.T01:intron 45.0%
CCAAGAAAAGCCGAGTTTGG+AGG + Chr7:53968284-53968303 MsG0780038847.01.T01:intron 50.0%
CGTGAGATCTCATGGCAACA+TGG + Chr7:53968212-53968231 MsG0780038847.01.T01:intron 50.0%
CTAACACCTAGTGGTCGGAT+TGG + Chr7:53969004-53969023 MsG0780038847.01.T01:CDS 50.0%
GAGATCTCATGGCAACATGG+TGG + Chr7:53968215-53968234 MsG0780038847.01.T01:intron 50.0%
GATCTCACGAGATACAGTGG+CGG - Chr7:53968201-53968220 None:intergenic 50.0%
! CCGAGTTTGGAGGTTTGGTA+AGG + Chr7:53968294-53968313 MsG0780038847.01.T01:intron 50.0%
! CCTCCAAACTCGGCTTTTCT+TGG - Chr7:53968287-53968306 None:intergenic 50.0%
! TGGTCGGATTGGAGAACCTA+AGG + Chr7:53969015-53969034 MsG0780038847.01.T01:CDS 50.0%
CCAGCTGCATCACACATCAC+TGG + Chr7:53969070-53969089 MsG0780038847.01.T01:CDS 55.0%
CCAGTGATGTGTGATGCAGC+TGG - Chr7:53969073-53969092 None:intergenic 55.0%
GGTTCTCCAATCCGACCACT+AGG - Chr7:53969013-53969032 None:intergenic 55.0%
!! GATACAGTGGCGGATCTCCT+TGG - Chr7:53968191-53968210 None:intergenic 55.0%
! CTCATGGCAACATGGTGGCG+TGG + Chr7:53968220-53968239 MsG0780038847.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 53968180 53969129 53968180 ID=MsG0780038847.01;Name=MsG0780038847.01
Chr7 mRNA 53968180 53969129 53968180 ID=MsG0780038847.01.T01;Parent=MsG0780038847.01;Name=MsG0780038847.01.T01;_AED=0.50;_eAED=0.58;_QI=0|0|0|1|0|0|2|0|67
Chr7 exon 53968180 53968212 53968180 ID=MsG0780038847.01.T01:exon:7551;Parent=MsG0780038847.01.T01
Chr7 exon 53968959 53969129 53968959 ID=MsG0780038847.01.T01:exon:7552;Parent=MsG0780038847.01.T01
Chr7 CDS 53968180 53968212 53968180 ID=MsG0780038847.01.T01:cds;Parent=MsG0780038847.01.T01
Chr7 CDS 53968959 53969129 53968959 ID=MsG0780038847.01.T01:cds;Parent=MsG0780038847.01.T01
Gene Sequence

>MsG0780038847.01.T01

ATGGGTCTCCAAGGAGATCCGCCACTGTATCTCAAATTAACTGGCACAATGGATAATGTTGTCGAGGATATGGTGTCTCTAACACCTAGTGGTCGGATTGGAGAACCTAAGGATATATCAGCATTGGCTGCTTTTCTTTGTCTTCCAGCTGCATCACACATCACTGGACAAATTATAGCTGCAGATGGAGGCTTCACTATTTAA

Protein sequence

>MsG0780038847.01.T01

MGLQGDPPLYLKLTGTMDNVVEDMVSLTPSGRIGEPKDISALAAFLCLPAASHITGQIIAADGGFTI*