AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009142.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009142.01.T01 MTR_2g054880 99.123 228 2 0 1 228 1 228 1.89e-170 474
MsG0280009142.01.T01 MTR_1g113940 44.762 210 100 6 18 219 3 204 1.44e-50 168
MsG0280009142.01.T01 MTR_4g119900 45.918 196 81 3 26 212 142 321 6.63e-49 168
MsG0280009142.01.T01 MTR_4g119900 45.918 196 81 3 26 212 142 321 7.71e-49 168
MsG0280009142.01.T01 MTR_2g436780 44.949 198 84 3 26 214 144 325 4.08e-48 166
MsG0280009142.01.T01 MTR_2g436780 44.949 198 84 3 26 214 144 325 4.99e-48 166
MsG0280009142.01.T01 MTR_6g091770 45.263 190 90 4 26 214 65 241 4.90e-47 160
MsG0280009142.01.T01 MTR_3g013960 38.991 218 98 5 25 223 255 456 8.45e-37 136
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009142.01.T01 AT1G04870 80.189 212 42 0 17 228 26 237 5.63e-131 375
MsG0280009142.01.T01 AT1G04870 75.373 134 33 0 95 228 1 134 2.43e-75 229
MsG0280009142.01.T01 AT3G20020 47.090 189 87 2 26 214 83 258 2.04e-51 172
MsG0280009142.01.T01 AT3G20020 47.090 189 87 2 26 214 83 258 2.21e-51 172
MsG0280009142.01.T01 AT4G29510 45.263 190 90 3 26 214 72 248 1.55e-47 161
MsG0280009142.01.T01 AT3G06930 41.860 215 113 5 2 212 121 327 3.89e-47 163
MsG0280009142.01.T01 AT3G06930 41.860 215 113 5 2 212 121 327 4.58e-47 163
MsG0280009142.01.T01 AT2G19670 48.684 152 77 1 26 176 48 199 5.15e-46 157
MsG0280009142.01.T01 AT5G49020 45.213 188 94 4 26 212 149 328 1.40e-45 159
MsG0280009142.01.T01 AT5G49020 45.213 188 94 4 26 212 151 330 1.55e-45 159
MsG0280009142.01.T01 AT3G20020 40.796 201 83 4 15 214 71 236 1.17e-41 146
MsG0280009142.01.T01 AT3G12270 39.048 210 100 5 25 222 244 437 1.08e-36 136

Find 62 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGCGTAGTCGACTTCTTT+GGG 0.258466 2:+46245448 None:intergenic
GAAAATTGTGTAGGTTGTTT+TGG 0.265556 2:-46245245 MsG0280009142.01.T01:intron
TTCTACTATCCCAGTCCTAA+TGG 0.297541 2:+46243093 None:intergenic
ATTGGCGTAGTCGACTTCTT+TGG 0.303065 2:+46245447 None:intergenic
GATGAAACAAAAGCCTTCTA+TGG 0.355790 2:-46243019 MsG0280009142.01.T01:CDS
AACGCCGCCGCTGGTGGCCG+AGG 0.392661 2:-46245481 MsG0280009142.01.T01:CDS
TTGGATGTTGGTGCTGGAAG+TGG 0.397747 2:-46245226 MsG0280009142.01.T01:CDS
GCTCATATCAACACCATAGA+AGG 0.404592 2:+46243006 None:intergenic
GGAGTAGGTGCAGAAGTAAT+TGG 0.411108 2:+46245429 None:intergenic
CAAACACCACTTTAAAGATA+AGG 0.429315 2:-46245332 MsG0280009142.01.T01:intron
CAGGTTGTTGAGGTTATTGA+GGG 0.437694 2:-46245094 MsG0280009142.01.T01:CDS
TCTACTATCCCAGTCCTAAT+GGG 0.449075 2:+46243094 None:intergenic
TGGATGTTGGTGCTGGAAGT+GGG 0.452123 2:-46245225 MsG0280009142.01.T01:CDS
GAAGTGGGATTCTTGCTATA+TGG 0.455599 2:-46245210 MsG0280009142.01.T01:CDS
GCATGTGGATGGCACCCATT+AGG 0.455721 2:-46243108 MsG0280009142.01.T01:CDS
CATACAAACAAACCTTTCTC+AGG 0.456902 2:+46245049 None:intergenic
GCAGGTTGTTGAGGTTATTG+AGG 0.459237 2:-46245095 MsG0280009142.01.T01:CDS
GACCGCTGGCTCAAACCAAC+AGG 0.460638 2:-46244145 MsG0280009142.01.T01:CDS
GATGTTACATTGCCTGAGAA+AGG 0.461263 2:-46245061 MsG0280009142.01.T01:intron
TTCCTCTGCTCCTCAGCAAA+AGG 0.461816 2:+46242971 None:intergenic
GTCAGGATTGGTTTGGCGGT+AGG 0.462470 2:+46245568 None:intergenic
TCATATGTGCTCGTGACCGC+TGG 0.468854 2:-46244159 MsG0280009142.01.T01:CDS
CTTTGGGGACGGCGGTGCCT+CGG 0.475277 2:+46245464 None:intergenic
GGGCGATTACCAGTCAGCTA+TGG 0.480043 2:-46243062 MsG0280009142.01.T01:CDS
AGCTCAAGCAGGTGCAAAGA+AGG 0.480270 2:-46245185 MsG0280009142.01.T01:CDS
TTAGGTCAGGATTGGTTTGG+CGG 0.494154 2:+46245564 None:intergenic
TGGGATAGTAGAACAAAAGT+TGG 0.503612 2:-46243083 MsG0280009142.01.T01:CDS
ACCAGTCAGCTATGGGCGAT+TGG 0.509747 2:-46243054 MsG0280009142.01.T01:CDS
TCTTCCGAAAACGCCGCCGC+TGG 0.511121 2:-46245490 MsG0280009142.01.T01:CDS
CTCCTGTTGGTTTGAGCCAG+CGG 0.511819 2:+46244143 None:intergenic
GGTGCCTCGGCCACCAGCGG+CGG 0.514143 2:+46245477 None:intergenic
TCCGAAAACGCCGCCGCTGG+TGG 0.516593 2:-46245487 MsG0280009142.01.T01:CDS
GGTTATTGAGGGTTCTGTTG+AGG 0.518633 2:-46245083 MsG0280009142.01.T01:CDS
AATACTTACATCACTCCTGT+TGG 0.521476 2:+46244130 None:intergenic
GCAGAGGAAATACTATCTAC+AGG 0.521867 2:-46242957 MsG0280009142.01.T01:CDS
TGGATGGCACCCATTAGGAC+TGG 0.527259 2:-46243103 MsG0280009142.01.T01:CDS
TGTTATCATCTCTGAATGGA+TGG 0.530239 2:-46244218 MsG0280009142.01.T01:CDS
GGCGATTACCAGTCAGCTAT+GGG 0.531624 2:-46243061 MsG0280009142.01.T01:CDS
GCCAATCGCCCATAGCTGAC+TGG 0.534934 2:+46243053 None:intergenic
TACTAACCTTATCTTTAAAG+TGG 0.534994 2:+46245326 None:intergenic
GCTCTCAGATCGTGTTCGTA+TGG 0.535538 2:-46245383 MsG0280009142.01.T01:CDS
GGCGGTGCCTCGGCCACCAG+CGG 0.543715 2:+46245474 None:intergenic
TAATCTTGAGCAGGTTGTTG+AGG 0.544159 2:-46245104 MsG0280009142.01.T01:CDS
GAAATCGAATAATCTTGAGC+AGG 0.548053 2:-46245113 MsG0280009142.01.T01:CDS
GCTATATGGTCAGCTCAAGC+AGG 0.548853 2:-46245196 MsG0280009142.01.T01:CDS
GGGATAGTAGAACAAAAGTT+GGG 0.555999 2:-46243082 MsG0280009142.01.T01:CDS
TGGCGTAGTCGACTTCTTTG+GGG 0.561352 2:+46245449 None:intergenic
TAGTCATGCTCGCATGTGGA+TGG 0.569852 2:-46243119 MsG0280009142.01.T01:CDS
TTGGTGATAGAGAAAGGAGT+AGG 0.571481 2:+46245414 None:intergenic
ATCCACATGCGAGCATGACT+AGG 0.578485 2:+46243121 None:intergenic
GTAGTCGACTTCTTTGGGGA+CGG 0.581667 2:+46245453 None:intergenic
GGATGGCACCCATTAGGACT+GGG 0.582000 2:-46243102 MsG0280009142.01.T01:CDS
ATCCTAGTCATGCTCGCATG+TGG 0.586013 2:-46243123 MsG0280009142.01.T01:CDS
TTGATGTTATCATCTCTGAA+TGG 0.604535 2:-46244222 MsG0280009142.01.T01:CDS
GGTTCTTACTCACTCAGCGG+TGG 0.619536 2:+46245522 None:intergenic
TGTTGAAGCTACTAAGATGT+CGG 0.627273 2:-46245155 MsG0280009142.01.T01:CDS
GTCGACTTCTTTGGGGACGG+CGG 0.634616 2:+46245456 None:intergenic
GCTGAGTGAGTAAGAACCAT+GGG 0.636916 2:-46245517 MsG0280009142.01.T01:exon
CGCTGAGTGAGTAAGAACCA+TGG 0.649117 2:-46245518 MsG0280009142.01.T01:exon
TGGTGTTGATATGAGCACTT+TGG 0.661843 2:-46242999 MsG0280009142.01.T01:CDS
GTTATCATCTCTGAATGGAT+GGG 0.664829 2:-46244217 MsG0280009142.01.T01:CDS
CATGGTTCTTACTCACTCAG+CGG 0.701342 2:+46245519 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTATGAATATTTCAAAA+AGG - Chr2:46243849-46243868 MsG0280009142.01.T01:intron 10.0%
!!! ATAAGAAAATATTGTTTTTT+CGG - Chr2:46244021-46244040 MsG0280009142.01.T01:intron 10.0%
!! AACATTTAACAATTCATATA+AGG + Chr2:46244191-46244210 None:intergenic 15.0%
!! TATACTATATATGAAAAATG+TGG - Chr2:46244558-46244577 MsG0280009142.01.T01:intron 15.0%
!! TTAACCATAAATTATCAATT+TGG + Chr2:46243962-46243981 None:intergenic 15.0%
!! TTATATGAATTGTTAAATGT+TGG - Chr2:46244190-46244209 MsG0280009142.01.T01:CDS 15.0%
!! TTATTAAGTGGTTAAATATA+GGG - Chr2:46243935-46243954 MsG0280009142.01.T01:intron 15.0%
!!! TAAGAAAATATTGTTTTTTC+GGG - Chr2:46244022-46244041 MsG0280009142.01.T01:intron 15.0%
!! GTTATTAAGTGGTTAAATAT+AGG - Chr2:46243934-46243953 MsG0280009142.01.T01:intron 20.0%
!! TAATGATAAGAAAATTGTGT+AGG - Chr2:46243242-46243261 MsG0280009142.01.T01:intron 20.0%
!!! ATTTTTACATTGACTTAACA+TGG - Chr2:46245146-46245165 MsG0280009142.01.T01:CDS 20.0%
!!! CTTTAGTTTTCAATAGAATA+GGG - Chr2:46244671-46244690 MsG0280009142.01.T01:intron 20.0%
!!! TATTTTCTTATAATAGTGAC+CGG + Chr2:46244012-46244031 None:intergenic 20.0%
!!! TCTTTAGTTTTCAATAGAAT+AGG - Chr2:46244670-46244689 MsG0280009142.01.T01:intron 20.0%
!!! TGTTATGATATTTGATAATG+CGG - Chr2:46243726-46243745 MsG0280009142.01.T01:intron 20.0%
! AAGTCAATGTAAAAATAGCA+TGG + Chr2:46245142-46245161 None:intergenic 25.0%
! AGTAACATTTGACAAAATCA+AGG + Chr2:46243876-46243895 None:intergenic 25.0%
! ATGAATTGTTAAATGTTGGT+TGG - Chr2:46244194-46244213 MsG0280009142.01.T01:CDS 25.0%
! ATTACCTATTTGAGAGTTTA+GGG - Chr2:46244100-46244119 MsG0280009142.01.T01:intron 25.0%
! CAAAACCTTACAACTTAAAA+GGG + Chr2:46243684-46243703 None:intergenic 25.0%
! TCAAAACCTTACAACTTAAA+AGG + Chr2:46243685-46243704 None:intergenic 25.0%
! TGACATGTATATCAATGATT+TGG - Chr2:46245218-46245237 MsG0280009142.01.T01:CDS 25.0%
! TTAGTATCCTTAACTTAACT+GGG - Chr2:46243187-46243206 MsG0280009142.01.T01:intron 25.0%
! TTTGTTAATGAGCAAAACTT+TGG - Chr2:46245298-46245317 MsG0280009142.01.T01:intron 25.0%
!! TACTAACCTTATCTTTAAAG+TGG + Chr2:46243173-46243192 None:intergenic 25.0%
!!! GATTCAGATTTGTTAACATT+AGG + Chr2:46245072-46245091 None:intergenic 25.0%
!!! TAAGGTTTTGAATTAAATCG+TGG - Chr2:46243694-46243713 MsG0280009142.01.T01:intron 25.0%
!!! TAGTTCTAGTTTAAGTTGTA+AGG - Chr2:46243478-46243497 MsG0280009142.01.T01:intron 25.0%
!!! TTAAGTTGTAAGGTTTTAGA+GGG - Chr2:46243488-46243507 MsG0280009142.01.T01:intron 25.0%
!!! TTTAAGTTGTAAGGTTTTAG+AGG - Chr2:46243487-46243506 MsG0280009142.01.T01:intron 25.0%
!!! TTTGATAATGCGGTTTTATT+TGG - Chr2:46243736-46243755 MsG0280009142.01.T01:intron 25.0%
AAATTTGTCTCCGAATTTGT+AGG - Chr2:46243805-46243824 MsG0280009142.01.T01:intron 30.0%
AACTTAATTGATACTTCCTC+CGG - Chr2:46243990-46244009 MsG0280009142.01.T01:intron 30.0%
ACTGTAACACTGTTGTAAAA+CGG - Chr2:46244779-46244798 MsG0280009142.01.T01:intron 30.0%
ATACAGACACAAACACTTTA+AGG + Chr2:46244245-46244264 None:intergenic 30.0%
ATGTATCTGGACATCAATTA+AGG - Chr2:46244051-46244070 MsG0280009142.01.T01:intron 30.0%
CTGACCTAAAAACATAAACT+TGG - Chr2:46242946-46242965 MsG0280009142.01.T01:CDS 30.0%
GATTACCTATTTGAGAGTTT+AGG - Chr2:46244099-46244118 MsG0280009142.01.T01:intron 30.0%
GGTTAATCAGTATTGTTAAG+TGG - Chr2:46244692-46244711 MsG0280009142.01.T01:intron 30.0%
GTTAGTATCCTTAACTTAAC+TGG - Chr2:46243186-46243205 MsG0280009142.01.T01:intron 30.0%
TAAACCCTAAACTCTCAAAT+AGG + Chr2:46244107-46244126 None:intergenic 30.0%
TAATCAGTATTGTTAAGTGG+TGG - Chr2:46244695-46244714 MsG0280009142.01.T01:intron 30.0%
TACAAATTCGGAGACAAATT+TGG + Chr2:46243806-46243825 None:intergenic 30.0%
TTGATGTTATCATCTCTGAA+TGG - Chr2:46244274-46244293 MsG0280009142.01.T01:intron 30.0%
! AATTTGGTGATTTACTCTCA+AGG + Chr2:46243790-46243809 None:intergenic 30.0%
! AGCATGGTAATGTGAAAATT+GGG + Chr2:46245126-46245145 None:intergenic 30.0%
! CAAACACCACTTTAAAGATA+AGG - Chr2:46243164-46243183 MsG0280009142.01.T01:intron 30.0%
! GAAAATTGTGTAGGTTGTTT+TGG - Chr2:46243251-46243270 MsG0280009142.01.T01:intron 30.0%
! GCAACCAAGTTTATGTTTTT+AGG + Chr2:46242953-46242972 None:intergenic 30.0%
! GGGTCCAAATTGATAATTTA+TGG - Chr2:46243955-46243974 MsG0280009142.01.T01:intron 30.0%
! TAGCATGGTAATGTGAAAAT+TGG + Chr2:46245127-46245146 None:intergenic 30.0%
! TATTTGAGAGTTTAGGGTTT+AGG - Chr2:46244106-46244125 MsG0280009142.01.T01:intron 30.0%
!! AAACACTTTAAGGCAATCAA+AGG + Chr2:46244235-46244254 None:intergenic 30.0%
!! AATGATTTGGTAAACTGTGA+AGG - Chr2:46245231-46245250 MsG0280009142.01.T01:CDS 30.0%
!! TTCTTTTTGGTGATAGAGAA+AGG + Chr2:46243091-46243110 None:intergenic 30.0%
!!! CAAGTTTATGTTTTTAGGTC+AGG + Chr2:46242948-46242967 None:intergenic 30.0%
!!! GTTTTTTTAATTCACCTCAG+TGG + Chr2:46244858-46244877 None:intergenic 30.0%
!!! TAAGTTGTAAGGTTTTAGAG+GGG - Chr2:46243489-46243508 MsG0280009142.01.T01:intron 30.0%
!!! TCTAGTTTTTCAAATGAGTG+AGG + Chr2:46244473-46244492 None:intergenic 30.0%
!!! TTATGTTTTTAGGTCAGGAT+TGG + Chr2:46242943-46242962 None:intergenic 30.0%
AAAAAACCCTCTTTCGTTTC+TGG + Chr2:46244401-46244420 None:intergenic 35.0%
AAAAACCCTCTTTCGTTTCT+GGG + Chr2:46244400-46244419 None:intergenic 35.0%
AATACTTACATCACTCCTGT+TGG + Chr2:46244369-46244388 None:intergenic 35.0%
ACATGGATAGCAGAAAAAAG+TGG + Chr2:46244821-46244840 None:intergenic 35.0%
AGCCTCTTGTGTAAAAAACA+GGG - Chr2:46243579-46243598 MsG0280009142.01.T01:intron 35.0%
ATACATGTCAGTATCTCTGT+TGG + Chr2:46245208-46245227 None:intergenic 35.0%
ATCCTTAACTTAACTGGGTT+TGG - Chr2:46243192-46243211 MsG0280009142.01.T01:intron 35.0%
ATGTGAAAATTGGGGTAGTT+AGG + Chr2:46245117-46245136 None:intergenic 35.0%
CATACAAACAAACCTTTCTC+AGG + Chr2:46243450-46243469 None:intergenic 35.0%
GAAATCGAATAATCTTGAGC+AGG - Chr2:46243383-46243402 MsG0280009142.01.T01:intron 35.0%
GATGAAACAAAAGCCTTCTA+TGG - Chr2:46245477-46245496 MsG0280009142.01.T01:CDS 35.0%
GGGATAGTAGAACAAAAGTT+GGG - Chr2:46245414-46245433 MsG0280009142.01.T01:CDS 35.0%
GGTTTCAGATGTCTTAGTTA+TGG + Chr2:46243769-46243788 None:intergenic 35.0%
GTTAATGAGCAAAACTTTGG+TGG - Chr2:46245301-46245320 MsG0280009142.01.T01:intron 35.0%
GTTATCATCTCTGAATGGAT+GGG - Chr2:46244279-46244298 MsG0280009142.01.T01:intron 35.0%
TCCTTAACTTAACTGGGTTT+GGG - Chr2:46243193-46243212 MsG0280009142.01.T01:intron 35.0%
TGGGATAGTAGAACAAAAGT+TGG - Chr2:46245413-46245432 MsG0280009142.01.T01:CDS 35.0%
TGTGAAAATTGGGGTAGTTA+GGG + Chr2:46245116-46245135 None:intergenic 35.0%
TGTTATCATCTCTGAATGGA+TGG - Chr2:46244278-46244297 MsG0280009142.01.T01:intron 35.0%
TGTTGAAGCTACTAAGATGT+CGG - Chr2:46243341-46243360 MsG0280009142.01.T01:intron 35.0%
TTTCTTATAATAGTGACCGG+AGG + Chr2:46244009-46244028 None:intergenic 35.0%
! AGTGGCTCTATTATAGTGAA+GGG + Chr2:46245039-46245058 None:intergenic 35.0%
! GCATGGTAATGTGAAAATTG+GGG + Chr2:46245125-46245144 None:intergenic 35.0%
! GGGACTAGATTGTTATTAAG+TGG - Chr2:46243923-46243942 MsG0280009142.01.T01:intron 35.0%
! GTTTTACAACAGTGTTACAG+TGG + Chr2:46244780-46244799 None:intergenic 35.0%
! TCTGGTTCATGAAAATGAGA+TGG - Chr2:46245256-46245275 MsG0280009142.01.T01:intron 35.0%
! TGGTGTTAATTGCAGAAACA+TGG + Chr2:46244838-46244857 None:intergenic 35.0%
! TTTTCAAATGAGTGAGGAGT+AGG + Chr2:46244467-46244486 None:intergenic 35.0%
!! TAGGGGTTTTGTGTTATGAA+GGG + Chr2:46245098-46245117 None:intergenic 35.0%
!! TTAGGGGTTTTGTGTTATGA+AGG + Chr2:46245099-46245118 None:intergenic 35.0%
!! TTGATAGCGTCCTACAAATT+CGG + Chr2:46243818-46243837 None:intergenic 35.0%
!! TTTAGGGTTTAGGACTAGTT+TGG - Chr2:46244116-46244135 MsG0280009142.01.T01:intron 35.0%
!! TTTCGGGTTACTTATGTATC+TGG - Chr2:46244038-46244057 MsG0280009142.01.T01:intron 35.0%
!!! GATCTGAGAGCATTTCTTTT+TGG + Chr2:46243104-46243123 None:intergenic 35.0%
!!! TACCCTGTTTTTTACACAAG+AGG + Chr2:46243584-46243603 None:intergenic 35.0%
!!! TTTTTAGGTCAGGATTGGTT+TGG + Chr2:46242938-46242957 None:intergenic 35.0%
ACATTAGGTGAATAGAGCAG+TGG + Chr2:46245057-46245076 None:intergenic 40.0%
ACCCAAACCCAGTTAAGTTA+AGG + Chr2:46243197-46243216 None:intergenic 40.0%
ACTGCGTACAATACACCAAA+TGG - Chr2:46243606-46243625 MsG0280009142.01.T01:intron 40.0%
AGTTTATCCGATAGTCTGTG+AGG - Chr2:46243902-46243921 MsG0280009142.01.T01:intron 40.0%
CAATTGTTGCCACAACATCA+AGG + Chr2:46244156-46244175 None:intergenic 40.0%
CAGCCTCTTGTGTAAAAAAC+AGG - Chr2:46243578-46243597 MsG0280009142.01.T01:intron 40.0%
CAGGTTGTTGAGGTTATTGA+GGG - Chr2:46243402-46243421 MsG0280009142.01.T01:intron 40.0%
CGTACAATACACCAAATGGT+GGG - Chr2:46243610-46243629 MsG0280009142.01.T01:intron 40.0%
GATAGAAAACTCTCCTCCAA+GGG - Chr2:46245009-46245028 MsG0280009142.01.T01:intron 40.0%
GATGTTACATTGCCTGAGAA+AGG - Chr2:46243435-46243454 MsG0280009142.01.T01:intron 40.0%
GCAGAGGAAATACTATCTAC+AGG - Chr2:46245539-46245558 MsG0280009142.01.T01:five_prime_UTR 40.0%
GCTCATATCAACACCATAGA+AGG + Chr2:46245493-46245512 None:intergenic 40.0%
GGCAATCAAAGGATACACAA+AGG + Chr2:46244224-46244243 None:intergenic 40.0%
GTAATGTGCTGGAAATAGCA+TGG + Chr2:46245186-46245205 None:intergenic 40.0%
GTGAAAATTGGGGTAGTTAG+GGG + Chr2:46245115-46245134 None:intergenic 40.0%
GTTGTTGTCATGTGACTGAA+AGG - Chr2:46243534-46243553 MsG0280009142.01.T01:intron 40.0%
GTTTATCCGATAGTCTGTGA+GGG - Chr2:46243903-46243922 MsG0280009142.01.T01:intron 40.0%
TAATCTTGAGCAGGTTGTTG+AGG - Chr2:46243392-46243411 MsG0280009142.01.T01:intron 40.0%
TACAACTTAAAAGGGCAACC+CGG + Chr2:46243676-46243695 None:intergenic 40.0%
TACGCAAAACCTTGATGTTG+TGG - Chr2:46244144-46244163 MsG0280009142.01.T01:CDS 40.0%
TCTACTATCCCAGTCCTAAT+GGG + Chr2:46245405-46245424 None:intergenic 40.0%
TTATCGTTCACGTTCTCGTA+CGG + Chr2:46244905-46244924 None:intergenic 40.0%
TTCTACTATCCCAGTCCTAA+TGG + Chr2:46245406-46245425 None:intergenic 40.0%
TTCTGCAATTAACACCACTG+AGG - Chr2:46244841-46244860 MsG0280009142.01.T01:intron 40.0%
TTCTTCCCAGAAACGAAAGA+GGG - Chr2:46244392-46244411 MsG0280009142.01.T01:intron 40.0%
TTTCTTCCCAGAAACGAAAG+AGG - Chr2:46244391-46244410 MsG0280009142.01.T01:intron 40.0%
! AACTTAACTGGGTTTGGGTT+TGG - Chr2:46243198-46243217 MsG0280009142.01.T01:intron 40.0%
! ACTTAACTGGGTTTGGGTTT+GGG - Chr2:46243199-46243218 MsG0280009142.01.T01:intron 40.0%
! AGCAAAACTTTGGTGGCTTT+CGG - Chr2:46245308-46245327 MsG0280009142.01.T01:intron 40.0%
! CAGTGGCTCTATTATAGTGA+AGG + Chr2:46245040-46245059 None:intergenic 40.0%
! GAAGTGGGATTCTTGCTATA+TGG - Chr2:46243286-46243305 MsG0280009142.01.T01:intron 40.0%
! TTGGTGATAGAGAAAGGAGT+AGG + Chr2:46243085-46243104 None:intergenic 40.0%
!! GGTTTTGTGTTATGAAGGGA+AGG + Chr2:46245094-46245113 None:intergenic 40.0%
!! TATCTCTGTTGGTAATGTGC+TGG + Chr2:46245197-46245216 None:intergenic 40.0%
!! TGGTAAACTGTGAAGGTATC+TGG - Chr2:46245238-46245257 MsG0280009142.01.T01:intron 40.0%
!! TGGTGTTGATATGAGCACTT+TGG - Chr2:46245497-46245516 MsG0280009142.01.T01:CDS 40.0%
!!! GGTTGCCCTTTTAAGTTGTA+AGG - Chr2:46243676-46243695 MsG0280009142.01.T01:intron 40.0%
!!! GTGTAGGTTGTTTTGGATGT+TGG - Chr2:46243258-46243277 MsG0280009142.01.T01:intron 40.0%
AAAGGTCACAGGTTAAGTCC+TGG - Chr2:46243552-46243571 MsG0280009142.01.T01:intron 45.0%
CATGGTTCTTACTCACTCAG+CGG + Chr2:46242980-46242999 None:intergenic 45.0%
CGATAGAAAACTCTCCTCCA+AGG - Chr2:46245008-46245027 MsG0280009142.01.T01:intron 45.0%
GCAGGTTGTTGAGGTTATTG+AGG - Chr2:46243401-46243420 MsG0280009142.01.T01:intron 45.0%
GCGTACAATACACCAAATGG+TGG - Chr2:46243609-46243628 MsG0280009142.01.T01:intron 45.0%
GCTGAGTGAGTAAGAACCAT+GGG - Chr2:46242979-46242998 MsG0280009142.01.T01:CDS 45.0%
GGAGTAGGTGCAGAAGTAAT+TGG + Chr2:46243070-46243089 None:intergenic 45.0%
GGTTATTGAGGGTTCTGTTG+AGG - Chr2:46243413-46243432 MsG0280009142.01.T01:intron 45.0%
TATAGTGAAGGGCAAACCCT+TGG + Chr2:46245028-46245047 None:intergenic 45.0%
TCATGTGACTGAAAGGTCAC+AGG - Chr2:46243541-46243560 MsG0280009142.01.T01:intron 45.0%
TCTAGTCCCTCACAGACTAT+CGG + Chr2:46243912-46243931 None:intergenic 45.0%
! ATTGGCGTAGTCGACTTCTT+TGG + Chr2:46243052-46243071 None:intergenic 45.0%
! TTACACAAGAGGCTGTTTCC+AGG + Chr2:46243573-46243592 None:intergenic 45.0%
! TTGGCGTAGTCGACTTCTTT+GGG + Chr2:46243051-46243070 None:intergenic 45.0%
! TTTGGCAAAGCCTTTTGCTG+AGG - Chr2:46245515-46245534 MsG0280009142.01.T01:exon 45.0%
!! TTAGGTCAGGATTGGTTTGG+CGG + Chr2:46242935-46242954 None:intergenic 45.0%
!!! AGGTTTTAGAGGGGTAACCT+TGG - Chr2:46243498-46243517 MsG0280009142.01.T01:intron 45.0%
!!! GTTGTTTTGGATGTTGGTGC+TGG - Chr2:46243264-46243283 MsG0280009142.01.T01:intron 45.0%
!!! TTTAAAAATATTTATTTTGT+AGG - Chr2:46245349-46245368 MsG0280009142.01.T01:CDS 5.0%
AGCTCAAGCAGGTGCAAAGA+AGG - Chr2:46243311-46243330 MsG0280009142.01.T01:intron 50.0%
ATCCACATGCGAGCATGACT+AGG + Chr2:46245378-46245397 None:intergenic 50.0%
ATCCTAGTCATGCTCGCATG+TGG - Chr2:46245373-46245392 MsG0280009142.01.T01:CDS 50.0%
CAACTTTACCAGTTGCGCCA+AGG + Chr2:46243518-46243537 None:intergenic 50.0%
CGCTGAGTGAGTAAGAACCA+TGG - Chr2:46242978-46242997 MsG0280009142.01.T01:CDS 50.0%
GCTATATGGTCAGCTCAAGC+AGG - Chr2:46243300-46243319 MsG0280009142.01.T01:intron 50.0%
GCTCTCAGATCGTGTTCGTA+TGG - Chr2:46243113-46243132 MsG0280009142.01.T01:CDS 50.0%
GGCGATTACCAGTCAGCTAT+GGG - Chr2:46245435-46245454 MsG0280009142.01.T01:CDS 50.0%
TAGTCATGCTCGCATGTGGA+TGG - Chr2:46245377-46245396 MsG0280009142.01.T01:CDS 50.0%
TTCCTCTGCTCCTCAGCAAA+AGG + Chr2:46245528-46245547 None:intergenic 50.0%
! GTAGTCGACTTCTTTGGGGA+CGG + Chr2:46243046-46243065 None:intergenic 50.0%
! TGGCGTAGTCGACTTCTTTG+GGG + Chr2:46243050-46243069 None:intergenic 50.0%
!! TGGATGTTGGTGCTGGAAGT+GGG - Chr2:46243271-46243290 MsG0280009142.01.T01:intron 50.0%
!! TTGGATGTTGGTGCTGGAAG+TGG - Chr2:46243270-46243289 MsG0280009142.01.T01:intron 50.0%
!!! GTTTTCGGAAGAAGTGCCCA+TGG + Chr2:46242998-46243017 None:intergenic 50.0%
ACCAGTCAGCTATGGGCGAT+TGG - Chr2:46245442-46245461 MsG0280009142.01.T01:CDS 55.0%
ACTAAAGCTCCCGCATACGC+AGG + Chr2:46243650-46243669 None:intergenic 55.0%
AGTGAAGGGCAAACCCTTGG+AGG + Chr2:46245025-46245044 None:intergenic 55.0%
CTAAAGCTCCCGCATACGCA+GGG + Chr2:46243649-46243668 None:intergenic 55.0%
GCATGTGGATGGCACCCATT+AGG - Chr2:46245388-46245407 MsG0280009142.01.T01:CDS 55.0%
GGATGGCACCCATTAGGACT+GGG - Chr2:46245394-46245413 MsG0280009142.01.T01:CDS 55.0%
GGGCGATTACCAGTCAGCTA+TGG - Chr2:46245434-46245453 MsG0280009142.01.T01:CDS 55.0%
GGTTCTTACTCACTCAGCGG+TGG + Chr2:46242977-46242996 None:intergenic 55.0%
TCATATGTGCTCGTGACCGC+TGG - Chr2:46244337-46244356 MsG0280009142.01.T01:intron 55.0%
TGGATGGCACCCATTAGGAC+TGG - Chr2:46245393-46245412 MsG0280009142.01.T01:CDS 55.0%
! AGCCTTTTGCTGAGGAGCAG+AGG - Chr2:46245523-46245542 MsG0280009142.01.T01:five_prime_UTR 55.0%
! ATGCGGGAGCTTTAGTGCAC+CGG - Chr2:46243654-46243673 MsG0280009142.01.T01:intron 55.0%
!! CTCCTGTTGGTTTGAGCCAG+CGG + Chr2:46244356-46244375 None:intergenic 55.0%
AGGGGTAACCTTGGCGCAAC+TGG - Chr2:46243507-46243526 MsG0280009142.01.T01:intron 60.0%
CAGGAAGGGGTCCCACCATT+TGG + Chr2:46243624-46243643 None:intergenic 60.0%
CATACGCAGGGTCCAGGAAG+GGG + Chr2:46243637-46243656 None:intergenic 60.0%
CTTCCTGGACCCTGCGTATG+CGG - Chr2:46243637-46243656 MsG0280009142.01.T01:intron 60.0%
GCATACGCAGGGTCCAGGAA+GGG + Chr2:46243638-46243657 None:intergenic 60.0%
GCCAATCGCCCATAGCTGAC+TGG + Chr2:46245446-46245465 None:intergenic 60.0%
TTCCTGGACCCTGCGTATGC+GGG - Chr2:46243638-46243657 MsG0280009142.01.T01:intron 60.0%
! CAAATGGTGGGACCCCTTCC+TGG - Chr2:46243622-46243641 MsG0280009142.01.T01:intron 60.0%
! GTCGACTTCTTTGGGGACGG+CGG + Chr2:46243043-46243062 None:intergenic 60.0%
! TGCGGGAGCTTTAGTGCACC+GGG - Chr2:46243655-46243674 MsG0280009142.01.T01:intron 60.0%
!! GACCGCTGGCTCAAACCAAC+AGG - Chr2:46244351-46244370 MsG0280009142.01.T01:intron 60.0%
CGCATACGCAGGGTCCAGGA+AGG + Chr2:46243639-46243658 None:intergenic 65.0%
TCTTCCGAAAACGCCGCCGC+TGG - Chr2:46243006-46243025 MsG0280009142.01.T01:CDS 65.0%
CTCCCGCATACGCAGGGTCC+AGG + Chr2:46243643-46243662 None:intergenic 70.0%
TCCGAAAACGCCGCCGCTGG+TGG - Chr2:46243009-46243028 MsG0280009142.01.T01:CDS 70.0%
! CTTTGGGGACGGCGGTGCCT+CGG + Chr2:46243035-46243054 None:intergenic 70.0%
! GCCACCAGCGGCGGCGTTTT+CGG + Chr2:46243013-46243032 None:intergenic 70.0%
! AACGCCGCCGCTGGTGGCCG+AGG - Chr2:46243015-46243034 MsG0280009142.01.T01:CDS 80.0%
! GGCGGTGCCTCGGCCACCAG+CGG + Chr2:46243025-46243044 None:intergenic 80.0%
! GGTGCCTCGGCCACCAGCGG+CGG + Chr2:46243022-46243041 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr2 gene 46242931 46245587 46242931 ID=MsG0280009142.01;Name=MsG0280009142.01
Chr2 mRNA 46242931 46245587 46242931 ID=MsG0280009142.01.T01;Parent=MsG0280009142.01;Name=MsG0280009142.01.T01;_AED=0.48;_eAED=0.49;_QI=66|1|0.75|1|0.66|1|4|0|234
Chr2 exon 46245333 46245587 46245333 ID=MsG0280009142.01.T01:exon:5114;Parent=MsG0280009142.01.T01
Chr2 exon 46245062 46245254 46245062 ID=MsG0280009142.01.T01:exon:5115;Parent=MsG0280009142.01.T01
Chr2 exon 46244139 46244244 46244139 ID=MsG0280009142.01.T01:exon:5116;Parent=MsG0280009142.01.T01
Chr2 exon 46242931 46243147 46242931 ID=MsG0280009142.01.T01:exon:5117;Parent=MsG0280009142.01.T01
Chr2 five_prime_UTR 46245522 46245587 46245522 ID=MsG0280009142.01.T01:five_prime_utr;Parent=MsG0280009142.01.T01
Chr2 CDS 46245333 46245521 46245333 ID=MsG0280009142.01.T01:cds;Parent=MsG0280009142.01.T01
Chr2 CDS 46245062 46245254 46245062 ID=MsG0280009142.01.T01:cds;Parent=MsG0280009142.01.T01
Chr2 CDS 46244139 46244244 46244139 ID=MsG0280009142.01.T01:cds;Parent=MsG0280009142.01.T01
Chr2 CDS 46242931 46243147 46242931 ID=MsG0280009142.01.T01:cds;Parent=MsG0280009142.01.T01
Gene Sequence

>MsG0280009142.01.T01

ATGGGCACTTCTTCCGAAAACGCCGCCGCTGGTGGCCGAGGCACCGCCGTCCCCAAAGAAGTCGACTACGCCAATTACTTCTGCACCTACTCCTTTCTCTATCACCAAAAAGAAATGCTCTCAGATCGTGTTCGTATGGATGCTTACTTCAACGCTGTTTTTGAAAACAAACACCACTTTAAAGATAAGGTTGTTTTGGATGTTGGTGCTGGAAGTGGGATTCTTGCTATATGGTCAGCTCAAGCAGGTGCAAAGAAGGTTTATGCTGTTGAAGCTACTAAGATGTCGGAACATGCTCGTGCGCTTGTGAAATCGAATAATCTTGAGCAGGTTGTTGAGGTTATTGAGGGTTCTGTTGAGGATGTTACATTGCCTGAGAAAGTTGATGTTATCATCTCTGAATGGATGGGTTATTTTCTTTTGCGAGAATCTATGTTTGATTCTGTCATATGTGCTCGTGACCGCTGGCTCAAACCAACAGGAGTGATGTATCCTAGTCATGCTCGCATGTGGATGGCACCCATTAGGACTGGGATAGTAGAACAAAAGTTGGGCGATTACCAGTCAGCTATGGGCGATTGGCACAACTTTGTCGATGAAACAAAAGCCTTCTATGGTGTTGATATGAGCACTTTGGCAAAGCCTTTTGCTGAGGAGCAGAGGAAATACTATCTACAGGCAAGTCATATACTTTGTACATCTTAA
Protein sequence

>MsG0280009142.01.T01

MGTSSENAAAGGRGTAVPKEVDYANYFCTYSFLYHQKEMLSDRVRMDAYFNAVFENKHHFKDKVVLDVGAGSGILAIWSAQAGAKKVYAVEATKMSEHARALVKSNNLEQVVEVIEGSVEDVTLPEKVDVIISEWMGYFLLRESMFDSVICARDRWLKPTGVMYPSHARMWMAPIRTGIVEQKLGDYQSAMGDWHNFVDETKAFYGVDMSTLAKPFAEEQRKYYLQASHILCTS*