AlfalfaGEDB | MsG0280009142.01

M. sativa cultivar ZhongmuNo.1 / MsG0280009142.01

KEGG: K11434
GO: GO:0006479 GO:0008168
PFAM: PF06325

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009142.01.T01	MCH80342.1	96.581	234	6	1	1	232	1	234	1.35E-167	472

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009142.01.T01	Q9MAT5	80.189	212	42	0	17	228	26	237	5.53E-130	375

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009142.01.T01	A0A072V8M2	99.123	228	2	0	1	228	1	228	7.47e-167	474
MsG0280009142.01.T01	A0A392LZ82	96.581	234	6	1	1	232	1	234	6.44e-168	472

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048944.01	MsG0280009142.01	0.867929	9.521640e-66	3.382243e-62
MsG0180000882.01	MsG0280009142.01	0.829626	4.634436e-55	4.769225e-52
MsG0180004991.01	MsG0280009142.01	0.816635	4.995178e-52	3.558579e-49
MsG0180005337.01	MsG0280009142.01	0.816218	6.194458e-52	4.363126e-49
MsG0280006305.01	MsG0280009142.01	0.841338	5.049889e-58	7.396399e-55
MsG0280009111.01	MsG0280009142.01	0.831798	1.360824e-55	1.492800e-52
MsG0280009141.01	MsG0280009142.01	0.828997	6.585489e-55	6.652614e-52
MsG0280009142.01	MsG0380017330.01	0.805668	1.194753e-49	6.358368e-47
MsG0280009142.01	MsG0380017836.01	0.835436	1.680310e-56	2.054634e-53
MsG0280009142.01	MsG0480023240.01	0.801722	7.873255e-49	3.786936e-46

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009142.01.T01	MTR_2g054880	99.123	228	2	0	1	228	1	228	1.89e-170	474
MsG0280009142.01.T01	MTR_1g113940	44.762	210	100	6	18	219	3	204	1.44e-50	168
MsG0280009142.01.T01	MTR_4g119900	45.918	196	81	3	26	212	142	321	6.63e-49	168
MsG0280009142.01.T01	MTR_4g119900	45.918	196	81	3	26	212	142	321	7.71e-49	168
MsG0280009142.01.T01	MTR_2g436780	44.949	198	84	3	26	214	144	325	4.08e-48	166
MsG0280009142.01.T01	MTR_2g436780	44.949	198	84	3	26	214	144	325	4.99e-48	166
MsG0280009142.01.T01	MTR_6g091770	45.263	190	90	4	26	214	65	241	4.90e-47	160
MsG0280009142.01.T01	MTR_3g013960	38.991	218	98	5	25	223	255	456	8.45e-37	136

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009142.01.T01	AT1G04870	80.189	212	42	0	17	228	26	237	5.63e-131	375
MsG0280009142.01.T01	AT1G04870	75.373	134	33	0	95	228	1	134	2.43e-75	229
MsG0280009142.01.T01	AT3G20020	47.090	189	87	2	26	214	83	258	2.04e-51	172
MsG0280009142.01.T01	AT3G20020	47.090	189	87	2	26	214	83	258	2.21e-51	172
MsG0280009142.01.T01	AT4G29510	45.263	190	90	3	26	214	72	248	1.55e-47	161
MsG0280009142.01.T01	AT3G06930	41.860	215	113	5	2	212	121	327	3.89e-47	163
MsG0280009142.01.T01	AT3G06930	41.860	215	113	5	2	212	121	327	4.58e-47	163
MsG0280009142.01.T01	AT2G19670	48.684	152	77	1	26	176	48	199	5.15e-46	157
MsG0280009142.01.T01	AT5G49020	45.213	188	94	4	26	212	149	328	1.40e-45	159
MsG0280009142.01.T01	AT5G49020	45.213	188	94	4	26	212	151	330	1.55e-45	159

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Find 62 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACGCCGCCGCTGGTGGCCG+AGG	0.392661	2:-46245481	MsG0280009142.01.T01:CDS
AATACTTACATCACTCCTGT+TGG	0.521476	2:+46244130	None:intergenic
ACCAGTCAGCTATGGGCGAT+TGG	0.509747	2:-46243054	MsG0280009142.01.T01:CDS
AGCTCAAGCAGGTGCAAAGA+AGG	0.480270	2:-46245185	MsG0280009142.01.T01:CDS
ATCCACATGCGAGCATGACT+AGG	0.578485	2:+46243121	None:intergenic
ATCCTAGTCATGCTCGCATG+TGG	0.586013	2:-46243123	MsG0280009142.01.T01:CDS
ATTGGCGTAGTCGACTTCTT+TGG	0.303065	2:+46245447	None:intergenic
CAAACACCACTTTAAAGATA+AGG	0.429315	2:-46245332	MsG0280009142.01.T01:intron
CAGGTTGTTGAGGTTATTGA+GGG	0.437694	2:-46245094	MsG0280009142.01.T01:CDS
CATACAAACAAACCTTTCTC+AGG	0.456902	2:+46245049	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATTTGTCTCCGAATTTGT+AGG	-	Chr2:46243805-46243824	MsG0280009142.01.T01:intron	30.0%
AACTTAATTGATACTTCCTC+CGG	-	Chr2:46243990-46244009	MsG0280009142.01.T01:intron	30.0%
ACTGTAACACTGTTGTAAAA+CGG	-	Chr2:46244779-46244798	MsG0280009142.01.T01:intron	30.0%
ATACAGACACAAACACTTTA+AGG	+	Chr2:46244245-46244264	None:intergenic	30.0%
ATGTATCTGGACATCAATTA+AGG	-	Chr2:46244051-46244070	MsG0280009142.01.T01:intron	30.0%
CTGACCTAAAAACATAAACT+TGG	-	Chr2:46242946-46242965	MsG0280009142.01.T01:CDS	30.0%
GATTACCTATTTGAGAGTTT+AGG	-	Chr2:46244099-46244118	MsG0280009142.01.T01:intron	30.0%
GGTTAATCAGTATTGTTAAG+TGG	-	Chr2:46244692-46244711	MsG0280009142.01.T01:intron	30.0%
GTTAGTATCCTTAACTTAAC+TGG	-	Chr2:46243186-46243205	MsG0280009142.01.T01:intron	30.0%
TAAACCCTAAACTCTCAAAT+AGG	+	Chr2:46244107-46244126	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	46242931	46245587	46242931	ID=MsG0280009142.01;Name=MsG0280009142.01
Chr2	mRNA	46242931	46245587	46242931	ID=MsG0280009142.01.T01;Parent=MsG0280009142.01;Name=MsG0280009142.01.T01;_AED=0.48;_eAED=0.49;_QI=66\|1\|0.75\|1\|0.66\|1\|4\|0\|234
Chr2	exon	46245333	46245587	46245333	ID=MsG0280009142.01.T01:exon:5114;Parent=MsG0280009142.01.T01
Chr2	exon	46245062	46245254	46245062	ID=MsG0280009142.01.T01:exon:5115;Parent=MsG0280009142.01.T01
Chr2	exon	46244139	46244244	46244139	ID=MsG0280009142.01.T01:exon:5116;Parent=MsG0280009142.01.T01
Chr2	exon	46242931	46243147	46242931	ID=MsG0280009142.01.T01:exon:5117;Parent=MsG0280009142.01.T01
Chr2	five_prime_UTR	46245522	46245587	46245522	ID=MsG0280009142.01.T01:five_prime_utr;Parent=MsG0280009142.01.T01
Chr2	CDS	46245333	46245521	46245333	ID=MsG0280009142.01.T01:cds;Parent=MsG0280009142.01.T01
Chr2	CDS	46245062	46245254	46245062	ID=MsG0280009142.01.T01:cds;Parent=MsG0280009142.01.T01
Chr2	CDS	46244139	46244244	46244139	ID=MsG0280009142.01.T01:cds;Parent=MsG0280009142.01.T01
Chr2	CDS	46242931	46243147	46242931	ID=MsG0280009142.01.T01:cds;Parent=MsG0280009142.01.T01

Gene Sequence

>MsG0280009142.01.T01

ATGGGCACTTCTTCCGAAAACGCCGCCGCTGGTGGCCGAGGCACCGCCGTCCCCAAAGAAGTCGACTACGCCAATTACTTCTGCACCTACTCCTTTCTCTATCACCAAAAAGAAATGCTCTCAGATCGTGTTCGTATGGATGCTTACTTCAACGCTGTTTTTGAAAACAAACACCACTTTAAAGATAAGGTTGTTTTGGATGTTGGTGCTGGAAGTGGGATTCTTGCTATATGGTCAGCTCAAGCAGGTGCAAAGAAGGTTTATGCTGTTGAAGCTACTAAGATGTCGGAACATGCTCGTGCGCTTGTGAAATCGAATAATCTTGAGCAGGTTGTTGAGGTTATTGAGGGTTCTGTTGAGGATGTTACATTGCCTGAGAAAGTTGATGTTATCATCTCTGAATGGATGGGTTATTTTCTTTTGCGAGAATCTATGTTTGATTCTGTCATATGTGCTCGTGACCGCTGGCTCAAACCAACAGGAGTGATGTATCCTAGTCATGCTCGCATGTGGATGGCACCCATTAGGACTGGGATAGTAGAACAAAAGTTGGGCGATTACCAGTCAGCTATGGGCGATTGGCACAACTTTGTCGATGAAACAAAAGCCTTCTATGGTGTTGATATGAGCACTTTGGCAAAGCCTTTTGCTGAGGAGCAGAGGAAATACTATCTACAGGCAAGTCATATACTTTGTACATCTTAA

Protein sequence

>MsG0280009142.01.T01

MGTSSENAAAGGRGTAVPKEVDYANYFCTYSFLYHQKEMLSDRVRMDAYFNAVFENKHHFKDKVVLDVGAGSGILAIWSAQAGAKKVYAVEATKMSEHARALVKSNNLEQVVEVIEGSVEDVTLPEKVDVIISEWMGYFLLRESMFDSVICARDRWLKPTGVMYPSHARMWMAPIRTGIVEQKLGDYQSAMGDWHNFVDETKAFYGVDMSTLAKPFAEEQRKYYLQASHILCTS*