AlfalfaGEDB | MsG0280009591.01

M. sativa cultivar ZhongmuNo.1 / MsG0280009591.01

KEGG: K17771
GO: GO:0005742 GO:0030150
PFAM: PF08038

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009591.01.T02	AFK43929.1	97.222	36	1	0	1	36	35	70	4.03E-16	74.7
MsG0280009591.01.T01	AFK43929.1	98.571	70	1	0	1	70	1	70	7.12E-42	141

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009591.01.T01	Q9ASY8	65.385	52	18	0	19	70	24	75	1.12E-21	83.2
MsG0280009591.01.T02	O82067	80	35	7	0	2	36	38	72	2.16E-14	63.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009591.01.T01	I3T329	98.571	70	1	0	1	70	1	70	3.40e-42	141
MsG0280009591.01.T02	I3T329	97.222	36	1	0	1	36	35	70	1.92e-16	74.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000575.01	MsG0280009591.01	0.826633	2.437042e-54	2.298216e-51
MsG0180000743.01	MsG0280009591.01	0.803850	2.863599e-49	1.454377e-46
MsG0180004509.01	MsG0280009591.01	0.805041	1.616428e-49	8.464874e-47
MsG0180005426.01	MsG0280009591.01	0.807801	4.233496e-50	2.380361e-47
MsG0180005659.01	MsG0280009591.01	0.813521	2.454217e-51	1.606361e-48
MsG0180005851.01	MsG0280009591.01	0.821026	5.024365e-53	4.041461e-50
MsG0280006819.01	MsG0280009591.01	0.842892	1.959460e-58	3.013176e-55
MsG0280006836.01	MsG0280009591.01	0.815694	8.109185e-52	5.630266e-49
MsG0280009375.01	MsG0280009591.01	0.805055	1.605678e-49	8.411746e-47
MsG0280009591.01	MsG0280010995.01	0.835477	1.640199e-56	2.008028e-53
MsG0280009591.01	MsG0280011234.01	0.822653	2.110892e-53	1.777754e-50
MsG0280009591.01	MsG0380017382.01	0.813393	2.619110e-51	1.708353e-48
MsG0280009591.01	MsG0480018497.01	-0.812056	5.135985e-51	3.231873e-48
MsG0280009591.01	MsG0480022913.01	0.834104	3.634172e-56	4.269766e-53
MsG0280009591.01	MsG0480023399.01	0.807144	5.833250e-50	3.224262e-47
MsG0280009591.01	MsG0680032770.01	0.825385	4.824587e-54	4.390739e-51
MsG0280009591.01	MsG0680033699.01	0.812572	3.963278e-51	2.528824e-48
MsG0280009591.01	MsG0780038587.01	0.820209	7.741471e-53	6.086693e-50
MsG0280009591.01	MsG0780041147.01	0.808434	3.103041e-50	1.774118e-47
MsG0280009591.01	MsG0780041224.01	0.866382	2.970990e-65	9.993126e-62
MsG0280009591.01	MsG0880045704.01	-0.806497	7.993347e-50	4.345081e-47
MsG0280009591.01	MsG0880046879.01	0.809094	2.242371e-50	1.304538e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009591.01.T02	MTR_2g067370	97.222	36	1	0	1	36	35	70	4.88e-20	74.7
MsG0280009591.01.T02	MTR_3g462060	94.444	36	2	0	1	36	35	70	9.96e-20	73.9
MsG0280009591.01.T02	MTR_3g055500	91.667	36	3	0	1	36	35	70	1.81e-18	72.4
MsG0280009591.01.T02	MTR_3g061970	77.273	44	9	1	1	43	35	78	8.21e-17	67.4
MsG0280009591.01.T01	MTR_2g067370	98.571	70	1	0	1	70	1	70	8.62e-46	141
MsG0280009591.01.T01	MTR_3g462060	88.571	70	8	0	1	70	1	70	8.39e-41	128
MsG0280009591.01.T01	MTR_3g055500	85.714	70	10	0	1	70	1	70	2.44e-39	127
MsG0280009591.01.T01	MTR_3g061970	79.221	77	15	1	1	76	1	77	4.20e-37	120

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009591.01.T02	AT5G41685	69.444	36	11	1	36	40	75	2.19e-14	60.5
MsG0280009591.01.T02	AT1G64220	72.222	36	10	1	36	42	77	3.15e-13	57.8
MsG0280009591.01.T01	AT5G41685	65.385	52	18	19	70	24	75	1.14e-22	83.2
MsG0280009591.01.T01	AT1G64220	69.767	43	13	28	70	35	77	6.68e-18	70.9

Find 18 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCTGAGAAATTTGTGGTTT+TGG	0.210299	2:+54868558	None:intergenic
ACAAAGGTTATTGCTCACTA+TGG	0.348329	2:-54868621	MsG0280009591.01.T01:CDS
ATTCCTTTGATTATCATCAT+TGG	0.388372	2:-54868594	MsG0280009591.01.T01:CDS
CTGAGAAGCTGAGAAATTTG+TGG	0.449278	2:+54868552	None:intergenic
GAAAAGAAGAATGGCGCCTA+GGG	0.517130	2:-54868742	None:intergenic
CTGTGAAGGAATGGACAACA+TGG	0.524007	2:-54868656	MsG0280009591.01.T01:CDS
TGATCGATTCTGTGAAGGAA+TGG	0.539433	2:-54868665	MsG0280009591.01.T01:CDS
TTTGCTTTCAGAGAAACCCT+AGG	0.540311	2:+54868726	None:intergenic
AGGGTTTCTCTGAAAGCAAA+GGG	0.547703	2:-54868723	MsG0280009591.01.T01:CDS
AGAAAAGAAGAATGGCGCCT+AGG	0.558230	2:-54868743	None:intergenic
AATGTGCAACATCGATTACA+CGG	0.568459	2:+54868528	None:intergenic
TAGGGTTTCTCTGAAAGCAA+AGG	0.590299	2:-54868724	MsG0280009591.01.T01:CDS
ATACCAATGATGATAATCAA+AGG	0.596226	2:+54868591	None:intergenic
ATCAGTGATCGATTCTGTGA+AGG	0.598645	2:-54868670	MsG0280009591.01.T01:CDS
TGTGAAGGAATGGACAACAT+GGG	0.649737	2:-54868655	MsG0280009591.01.T01:CDS
ATGTGCAACATCGATTACAC+GGG	0.672040	2:+54868529	None:intergenic
ATGGGGTATGAAGAAAACAA+AGG	0.679010	2:-54868637	MsG0280009591.01.T01:CDS
GTGAAGGAATGGACAACATG+GGG	0.691902	2:-54868654	MsG0280009591.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATACCAATGATGATAATCAA+AGG	+	Chr2:54868656-54868675	None:intergenic	25.0%
!	ATTCCTTTGATTATCATCAT+TGG	-	Chr2:54868650-54868669	MsG0280009591.01.T01:CDS	25.0%
	ACAAAGGTTATTGCTCACTA+TGG	-	Chr2:54868623-54868642	MsG0280009591.01.T01:CDS	35.0%
	ATGGGGTATGAAGAAAACAA+AGG	-	Chr2:54868607-54868626	MsG0280009591.01.T01:CDS	35.0%
!	AATGTGCAACATCGATTACA+CGG	+	Chr2:54868719-54868738	None:intergenic	35.0%
!	AGCTGAGAAATTTGTGGTTT+TGG	+	Chr2:54868689-54868708	None:intergenic	35.0%
	CTGAGAAGCTGAGAAATTTG+TGG	+	Chr2:54868695-54868714	None:intergenic	40.0%
	TGTGAAGGAATGGACAACAT+GGG	-	Chr2:54868589-54868608	MsG0280009591.01.T01:CDS	40.0%
	TTTGCTTTCAGAGAAACCCT+AGG	+	Chr2:54868521-54868540	None:intergenic	40.0%
!	AGGGTTTCTCTGAAAGCAAA+GGG	-	Chr2:54868521-54868540	MsG0280009591.01.T01:CDS	40.0%
!	ATCAGTGATCGATTCTGTGA+AGG	-	Chr2:54868574-54868593	MsG0280009591.01.T01:CDS	40.0%
!	ATGTGCAACATCGATTACAC+GGG	+	Chr2:54868718-54868737	None:intergenic	40.0%
!	TAGGGTTTCTCTGAAAGCAA+AGG	-	Chr2:54868520-54868539	MsG0280009591.01.T01:CDS	40.0%
!	TGATCGATTCTGTGAAGGAA+TGG	-	Chr2:54868579-54868598	MsG0280009591.01.T01:CDS	40.0%
	CTGTGAAGGAATGGACAACA+TGG	-	Chr2:54868588-54868607	MsG0280009591.01.T01:CDS	45.0%
	GTGAAGGAATGGACAACATG+GGG	-	Chr2:54868590-54868609	MsG0280009591.01.T01:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	54868512	54868754	54868512	ID=MsG0280009591.01;Name=MsG0280009591.01
Chr2	mRNA	54868512	54868754	54868512	ID=MsG0280009591.01.T01;Parent=MsG0280009591.01;Name=MsG0280009591.01.T01;_AED=0.34;_eAED=0.34;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|80
Chr2	exon	54868512	54868754	54868512	ID=MsG0280009591.01.T01:exon:20690;Parent=MsG0280009591.01.T01
Chr2	CDS	54868512	54868754	54868512	ID=MsG0280009591.01.T01:cds;Parent=MsG0280009591.01.T01
Chr2	mRNA	54868512	54868652	54868512	ID=MsG0280009591.01.T02;Parent=MsG0280009591.01;Name=MsG0280009591.01.T02;_AED=0.41;_eAED=0.41;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|46
Chr2	exon	54868512	54868652	54868512	ID=MsG0280009591.01.T02:exon:20691;Parent=MsG0280009591.01.T02
Chr2	CDS	54868512	54868652	54868512	ID=MsG0280009591.01.T02:cds;Parent=MsG0280009591.01.T02

Gene Sequence

>MsG0280009591.01.T02

ATGAAGAAAACAAAGGTTATTGCTCACTATGGTTTCATTCCTTTGATTATCATCATTGGTATGAATTCTGATCCAAAACCACAAATTTCTCAGCTTCTCAGCCCGTGTAATCGATGTTGCACATTTCACTTGCTTTTCTGA

>MsG0280009591.01.T01

ATGGCGCCTAGGGTTTCTCTGAAAGCAAAGGGAAAGAGCTCAAAGAAGACAGCAGAAGATCGATCAGTGATCGATTCTGTGAAGGAATGGACAACATGGGGTATGAAGAAAACAAAGGTTATTGCTCACTATGGTTTCATTCCTTTGATTATCATCATTGGTATGAATTCTGATCCAAAACCACAAATTTCTCAGCTTCTCAGCCCGTGTAATCGATGTTGCACATTTCACTTGCTTTTCTGA

Protein sequence

>MsG0280009591.01.T02

MKKTKVIAHYGFIPLIIIIGMNSDPKPQISQLLSPCNRCCTFHLLF*

>MsG0280009591.01.T01

MAPRVSLKAKGKSSKKTAEDRSVIDSVKEWTTWGMKKTKVIAHYGFIPLIIIIGMNSDPKPQISQLLSPCNRCCTFHLLF*