Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022913.01.T01 | XP_006580103.1 | 83.721 | 129 | 20 | 1 | 1 | 129 | 1 | 128 | 6.69E-71 | 218 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022913.01.T01 | I1K3I2 | 83.721 | 129 | 20 | 1 | 1 | 129 | 1 | 128 | 3.20e-71 | 218 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048971.01 | MsG0480022913.01 | 0.830245 | 3.272965e-55 | 3.429324e-52 |
| MsG0180000139.01 | MsG0480022913.01 | 0.874795 | 5.107987e-68 | 2.330320e-64 |
| MsG0180000339.01 | MsG0480022913.01 | 0.833646 | 4.732417e-56 | 5.483860e-53 |
| MsG0180000575.01 | MsG0480022913.01 | 0.869372 | 3.254057e-66 | 1.218452e-62 |
| MsG0180000614.01 | MsG0480022913.01 | 0.805943 | 1.045777e-49 | 5.605109e-47 |
| MsG0180000743.01 | MsG0480022913.01 | 0.852516 | 4.385018e-61 | 9.185480e-58 |
| MsG0180002547.01 | MsG0480022913.01 | 0.829021 | 6.497331e-55 | 6.568291e-52 |
| MsG0180003651.01 | MsG0480022913.01 | 0.837995 | 3.739504e-57 | 4.941843e-54 |
| MsG0180003793.01 | MsG0480022913.01 | 0.820467 | 6.754986e-53 | 5.349295e-50 |
| MsG0180004129.01 | MsG0480022913.01 | 0.820448 | 6.822747e-53 | 5.400093e-50 |
| MsG0180004130.01 | MsG0480022913.01 | 0.829245 | 5.732949e-55 | 5.834971e-52 |
| MsG0180004481.01 | MsG0480022913.01 | 0.846966 | 1.558070e-59 | 2.727746e-56 |
| MsG0180004482.01 | MsG0480022913.01 | 0.843025 | 1.805536e-58 | 2.788000e-55 |
| MsG0180004509.01 | MsG0480022913.01 | 0.870233 | 1.703275e-66 | 6.577110e-63 |
| MsG0180004854.01 | MsG0480022913.01 | 0.818929 | 1.516437e-52 | 1.150773e-49 |
| MsG0180004861.01 | MsG0480022913.01 | 0.823922 | 1.067198e-53 | 9.315626e-51 |
| MsG0180005426.01 | MsG0480022913.01 | 0.810136 | 1.339450e-50 | 8.010118e-48 |
| MsG0180005659.01 | MsG0480022913.01 | 0.863476 | 2.422549e-64 | 7.352924e-61 |
| MsG0180005851.01 | MsG0480022913.01 | 0.812177 | 4.833449e-51 | 3.051663e-48 |
| MsG0280006422.01 | MsG0480022913.01 | 0.819645 | 1.041994e-52 | 8.064566e-50 |
| MsG0280010776.01 | MsG0480022913.01 | 0.842264 | 2.875565e-58 | 4.336274e-55 |
| MsG0280011339.01 | MsG0480022913.01 | 0.802296 | 6.000511e-49 | 2.928887e-46 |
| MsG0280011387.01 | MsG0480022913.01 | 0.806392 | 8.409562e-50 | 4.559031e-47 |
| MsG0380012073.01 | MsG0480022913.01 | 0.836130 | 1.120939e-56 | 1.399628e-53 |
| MsG0380012108.01 | MsG0480022913.01 | 0.810111 | 1.355701e-50 | 8.102585e-48 |
| MsG0480018477.01 | MsG0480022913.01 | 0.810129 | 1.343642e-50 | 8.033888e-48 |
| MsG0480021006.01 | MsG0480022913.01 | 0.821091 | 4.853434e-53 | 3.910841e-50 |
| MsG0480021453.01 | MsG0480022913.01 | 0.811309 | 7.465699e-51 | 4.605389e-48 |
| MsG0480022696.01 | MsG0480022913.01 | 0.801593 | 8.369949e-49 | 4.012644e-46 |
| MsG0480022795.01 | MsG0480022913.01 | 0.800987 | 1.113606e-48 | 5.258407e-46 |
| MsG0480022913.01 | MsG0480022960.01 | 0.834335 | 3.181407e-56 | 3.763926e-53 |
| MsG0480022913.01 | MsG0480023149.01 | 0.804649 | 1.951610e-49 | 1.011760e-46 |
| MsG0480022913.01 | MsG0480023221.01 | 0.839933 | 1.177671e-57 | 1.651467e-54 |
| MsG0480022913.01 | MsG0480023399.01 | 0.817551 | 3.109373e-52 | 2.271414e-49 |
| MsG0480022913.01 | MsG0480023771.01 | 0.864553 | 1.119379e-64 | 3.528484e-61 |
| MsG0480022913.01 | MsG0480023844.01 | 0.809745 | 1.625445e-50 | 9.622192e-48 |
| MsG0480022913.01 | MsG0480023968.01 | 0.823175 | 1.595768e-53 | 1.363834e-50 |
| MsG0480022913.01 | MsG0580027361.01 | 0.802824 | 4.669292e-49 | 2.310063e-46 |
| MsG0480022913.01 | MsG0580029132.01 | 0.821985 | 3.017979e-53 | 2.493453e-50 |
| MsG0480022913.01 | MsG0580029488.01 | 0.809918 | 1.492291e-50 | 8.873579e-48 |
| MsG0480022913.01 | MsG0680031488.01 | 0.836063 | 1.165547e-56 | 1.452352e-53 |
| MsG0480022913.01 | MsG0680031628.01 | 0.803596 | 3.232167e-49 | 1.630923e-46 |
| MsG0480022913.01 | MsG0680032770.01 | 0.812547 | 4.012275e-51 | 2.558569e-48 |
| MsG0480022913.01 | MsG0680032809.01 | 0.834171 | 3.496864e-56 | 4.116105e-53 |
| MsG0480022913.01 | MsG0680035888.01 | 0.826288 | 2.945138e-54 | 2.750221e-51 |
| MsG0480022913.01 | MsG0780036240.01 | 0.804342 | 2.261879e-49 | 1.163354e-46 |
| MsG0480022913.01 | MsG0780036546.01 | 0.846455 | 2.149482e-59 | 3.702125e-56 |
| MsG0480022913.01 | MsG0780038400.01 | 0.865993 | 3.946454e-65 | 1.309438e-61 |
| MsG0480022913.01 | MsG0780038587.01 | 0.846839 | 1.688105e-59 | 2.943378e-56 |
| MsG0480022913.01 | MsG0780039844.01 | 0.824561 | 7.549304e-54 | 6.711018e-51 |
| MsG0480022913.01 | MsG0780039848.01 | 0.819971 | 8.776434e-53 | 6.855204e-50 |
| MsG0480022913.01 | MsG0780039998.01 | 0.812147 | 4.907390e-51 | 3.095845e-48 |
| MsG0480022913.01 | MsG0780040511.01 | 0.807220 | 5.622194e-50 | 3.113813e-47 |
| MsG0480022913.01 | MsG0780041223.01 | 0.844320 | 8.133882e-59 | 1.308226e-55 |
| MsG0480022913.01 | MsG0780041224.01 | 0.872694 | 2.611737e-67 | 1.103390e-63 |
| MsG0480022913.01 | MsG0780041462.01 | 0.836369 | 9.743082e-57 | 1.225350e-53 |
| MsG0480022913.01 | MsG0880041967.01 | 0.827123 | 1.861183e-54 | 1.780277e-51 |
| MsG0480022913.01 | MsG0880042519.01 | 0.846592 | 1.971813e-59 | 3.410871e-56 |
| MsG0480022913.01 | MsG0880042991.01 | 0.820714 | 5.927560e-53 | 4.726171e-50 |
| MsG0480022913.01 | MsG0880043477.01 | 0.810457 | 1.141948e-50 | 6.886663e-48 |
| MsG0480022913.01 | MsG0880046575.01 | 0.806709 | 7.209902e-50 | 3.940529e-47 |
| MsG0480022913.01 | MsG0880046631.01 | 0.805604 | 1.232146e-49 | 6.546371e-47 |
| MsG0480022913.01 | MsG0880047427.01 | 0.843640 | 1.237531e-58 | 1.948771e-55 |
| MsG0380015027.01 | MsG0480022913.01 | 0.840997 | 6.204662e-58 | 8.992072e-55 |
| MsG0380015435.01 | MsG0480022913.01 | 0.821837 | 3.264648e-53 | 2.686168e-50 |
| MsG0380016067.01 | MsG0480022913.01 | 0.829487 | 5.007916e-55 | 5.132680e-52 |
| MsG0380016290.01 | MsG0480022913.01 | 0.826740 | 2.297803e-54 | 2.173515e-51 |
| MsG0380016559.01 | MsG0480022913.01 | 0.843353 | 1.476779e-58 | 2.304484e-55 |
| MsG0380016560.01 | MsG0480022913.01 | 0.837177 | 6.060818e-57 | 7.809979e-54 |
| MsG0380016775.01 | MsG0480022913.01 | 0.842940 | 1.902534e-58 | 2.929938e-55 |
| MsG0380016778.01 | MsG0480022913.01 | 0.827829 | 1.260308e-54 | 1.230574e-51 |
| MsG0380017382.01 | MsG0480022913.01 | 0.846846 | 1.680089e-59 | 2.930048e-56 |
| MsG0380017857.01 | MsG0480022913.01 | 0.809180 | 2.149500e-50 | 1.253399e-47 |
| MsG0380017947.01 | MsG0480022913.01 | 0.832083 | 1.157299e-55 | 1.280010e-52 |
| MsG0480018110.01 | MsG0480022913.01 | 0.801837 | 7.457361e-49 | 3.597571e-46 |
| MsG0280006483.01 | MsG0480022913.01 | 0.810149 | 1.330273e-50 | 7.958006e-48 |
| MsG0280006993.01 | MsG0480022913.01 | 0.855665 | 5.416604e-62 | 1.260071e-58 |
| MsG0280007787.01 | MsG0480022913.01 | 0.816133 | 6.469956e-52 | 4.546417e-49 |
| MsG0280008118.01 | MsG0480022913.01 | 0.808082 | 3.688886e-50 | 2.089529e-47 |
| MsG0280009375.01 | MsG0480022913.01 | 0.832512 | 9.060076e-56 | 1.014925e-52 |
| MsG0280009591.01 | MsG0480022913.01 | 0.834104 | 3.634172e-56 | 4.269766e-53 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022913.01.T01 | MTR_8g079498 | 97.938 | 97 | 2 | 0 | 16 | 112 | 16 | 112 | 2.91e-66 | 197 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022913.01.T01 | AT4G22000 | 68.376 | 117 | 36 | 1 | 1 | 117 | 1 | 116 | 4.92e-54 | 166 |
Find 33 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTTCATCTCCTTCCTTTC+AGG | 0.120263 | 4:-78493618 | None:intergenic |
| CGTAAAGAAGTTCTTCTTGC+TGG | 0.300933 | 4:+78493576 | MsG0480022913.01.T01:CDS |
| TTCTTCTTGCTGGAGAAGAT+TGG | 0.378509 | 4:+78493586 | MsG0480022913.01.T01:CDS |
| AGCTGCTAGTTCAGCGGTAA+AGG | 0.432878 | 4:+78493413 | MsG0480022913.01.T01:CDS |
| GAAAAGGACATAAGGTTGAT+AGG | 0.434665 | 4:+78493646 | MsG0480022913.01.T01:CDS |
| GGCAAACACTGCTAGGTTGA+TGG | 0.439655 | 4:+78493686 | MsG0480022913.01.T01:CDS |
| TTAGTATTAGTGCTCGGCAT+AGG | 0.464094 | 4:+78493544 | MsG0480022913.01.T01:CDS |
| ATGAGTTTGCCAAGGGAGTG+TGG | 0.500959 | 4:+78493490 | MsG0480022913.01.T01:CDS |
| TTGTAAGCAAGCAACATATC+CGG | 0.503383 | 4:-78493717 | None:intergenic |
| TAGTATTAGTGCTCGGCATA+GGG | 0.503438 | 4:+78493545 | MsG0480022913.01.T01:CDS |
| TGAGTTTGCCAAGGGAGTGT+GGG | 0.513187 | 4:+78493491 | MsG0480022913.01.T01:CDS |
| GAGATGAAGACGAGGCGAAA+AGG | 0.513253 | 4:+78493630 | MsG0480022913.01.T01:CDS |
| TTTGCTAACTTCTCGCGCCA+TGG | 0.520110 | 4:-78493459 | None:intergenic |
| AAGATAAATAGGGTTATGGC+AGG | 0.525263 | 4:+78493387 | None:intergenic |
| TGCCGAGCACTAATACTAAG+AGG | 0.532897 | 4:-78493540 | None:intergenic |
| GGTATTGGGAGCTAGGGGCA+TGG | 0.536258 | 4:+78493514 | MsG0480022913.01.T01:CDS |
| GAGTTTGCCAAGGGAGTGTG+GGG | 0.545739 | 4:+78493492 | MsG0480022913.01.T01:CDS |
| GACGAGGCGAAAAGGACATA+AGG | 0.547643 | 4:+78493638 | MsG0480022913.01.T01:CDS |
| AGAAGAGGGCAAACACTGCT+AGG | 0.550721 | 4:+78493679 | MsG0480022913.01.T01:CDS |
| AAAGTGGGCGAAGAAGATGA+AGG | 0.570139 | 4:+78493746 | MsG0480022913.01.T01:CDS |
| AAGATGAAGGAAGAAGAGAA+AGG | 0.572789 | 4:+78493759 | MsG0480022913.01.T01:CDS |
| CTACAGAGTATCAATATCCA+TGG | 0.573029 | 4:+78493442 | MsG0480022913.01.T01:CDS |
| CGAGAAGTTAGCAAAATACA+AGG | 0.575656 | 4:+78493467 | MsG0480022913.01.T01:CDS |
| TAGGATAGCTGCGGAGAAGA+GGG | 0.580076 | 4:+78493665 | MsG0480022913.01.T01:CDS |
| AGCCTCTTAGTATTAGTGCT+CGG | 0.583490 | 4:+78493538 | MsG0480022913.01.T01:CDS |
| TAGGTTGATGGATAAGATGC+CGG | 0.589131 | 4:+78493698 | MsG0480022913.01.T01:CDS |
| ATAGGATAGCTGCGGAGAAG+AGG | 0.590998 | 4:+78493664 | MsG0480022913.01.T01:CDS |
| ATACAAGGATGAGTTTGCCA+AGG | 0.595001 | 4:+78493482 | MsG0480022913.01.T01:CDS |
| TACAAGGATGAGTTTGCCAA+GGG | 0.634320 | 4:+78493483 | MsG0480022913.01.T01:CDS |
| TAAGGTTGATAGGATAGCTG+CGG | 0.683219 | 4:+78493656 | MsG0480022913.01.T01:CDS |
| AGGAAAAGCTGCTAGTTCAG+CGG | 0.684482 | 4:+78493407 | MsG0480022913.01.T01:CDS |
| AAAGGAAGGAGATGAAGACG+AGG | 0.696241 | 4:+78493622 | MsG0480022913.01.T01:CDS |
| AGTTTGCCAAGGGAGTGTGG+GGG | 0.755828 | 4:+78493493 | MsG0480022913.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGCTTACAAGAAGAAAAAGT+GGG | + | Chr4:78493731-78493750 | MsG0480022913.01.T01:CDS | 30.0% | |
| TTGCTTACAAGAAGAAAAAG+TGG | + | Chr4:78493730-78493749 | MsG0480022913.01.T01:CDS | 30.0% | |
| AAGATGAAGGAAGAAGAGAA+AGG | + | Chr4:78493759-78493778 | MsG0480022913.01.T01:CDS | 35.0% | |
| CGAGAAGTTAGCAAAATACA+AGG | + | Chr4:78493467-78493486 | MsG0480022913.01.T01:CDS | 35.0% | |
| TTGTAAGCAAGCAACATATC+CGG | - | Chr4:78493720-78493739 | None:intergenic | 35.0% | |
| ! | ATTGGACTTTTGATCCTGAA+AGG | + | Chr4:78493604-78493623 | MsG0480022913.01.T01:CDS | 35.0% |
| ! | CTACAGAGTATCAATATCCA+TGG | + | Chr4:78493442-78493461 | MsG0480022913.01.T01:CDS | 35.0% |
| ! | GAAAAGGACATAAGGTTGAT+AGG | + | Chr4:78493646-78493665 | MsG0480022913.01.T01:CDS | 35.0% |
| AGCCTCTTAGTATTAGTGCT+CGG | + | Chr4:78493538-78493557 | MsG0480022913.01.T01:CDS | 40.0% | |
| ATACAAGGATGAGTTTGCCA+AGG | + | Chr4:78493482-78493501 | MsG0480022913.01.T01:CDS | 40.0% | |
| CGTAAAGAAGTTCTTCTTGC+TGG | + | Chr4:78493576-78493595 | MsG0480022913.01.T01:CDS | 40.0% | |
| TACAAGGATGAGTTTGCCAA+GGG | + | Chr4:78493483-78493502 | MsG0480022913.01.T01:CDS | 40.0% | |
| TAGTATTAGTGCTCGGCATA+GGG | + | Chr4:78493545-78493564 | MsG0480022913.01.T01:CDS | 40.0% | |
| TTAGTATTAGTGCTCGGCAT+AGG | + | Chr4:78493544-78493563 | MsG0480022913.01.T01:CDS | 40.0% | |
| TTCTTCTTGCTGGAGAAGAT+TGG | + | Chr4:78493586-78493605 | MsG0480022913.01.T01:CDS | 40.0% | |
| ! | GACTTTTGATCCTGAAAGGA+AGG | + | Chr4:78493608-78493627 | MsG0480022913.01.T01:CDS | 40.0% |
| !! | TAAGGTTGATAGGATAGCTG+CGG | + | Chr4:78493656-78493675 | MsG0480022913.01.T01:CDS | 40.0% |
| !! | TAGGTTGATGGATAAGATGC+CGG | + | Chr4:78493698-78493717 | MsG0480022913.01.T01:CDS | 40.0% |
| AAAGGAAGGAGATGAAGACG+AGG | + | Chr4:78493622-78493641 | MsG0480022913.01.T01:CDS | 45.0% | |
| AAAGTGGGCGAAGAAGATGA+AGG | + | Chr4:78493746-78493765 | MsG0480022913.01.T01:CDS | 45.0% | |
| AGGAAAAGCTGCTAGTTCAG+CGG | + | Chr4:78493407-78493426 | MsG0480022913.01.T01:CDS | 45.0% | |
| GTCTTCATCTCCTTCCTTTC+AGG | - | Chr4:78493621-78493640 | None:intergenic | 45.0% | |
| TGCCGAGCACTAATACTAAG+AGG | - | Chr4:78493543-78493562 | None:intergenic | 45.0% | |
| AGAAGAGGGCAAACACTGCT+AGG | + | Chr4:78493679-78493698 | MsG0480022913.01.T01:CDS | 50.0% | |
| AGCTGCTAGTTCAGCGGTAA+AGG | + | Chr4:78493413-78493432 | MsG0480022913.01.T01:CDS | 50.0% | |
| ATAGGATAGCTGCGGAGAAG+AGG | + | Chr4:78493664-78493683 | MsG0480022913.01.T01:CDS | 50.0% | |
| ATGAGTTTGCCAAGGGAGTG+TGG | + | Chr4:78493490-78493509 | MsG0480022913.01.T01:CDS | 50.0% | |
| GACGAGGCGAAAAGGACATA+AGG | + | Chr4:78493638-78493657 | MsG0480022913.01.T01:CDS | 50.0% | |
| GAGATGAAGACGAGGCGAAA+AGG | + | Chr4:78493630-78493649 | MsG0480022913.01.T01:CDS | 50.0% | |
| TAGGATAGCTGCGGAGAAGA+GGG | + | Chr4:78493665-78493684 | MsG0480022913.01.T01:CDS | 50.0% | |
| TTTGCTAACTTCTCGCGCCA+TGG | - | Chr4:78493462-78493481 | None:intergenic | 50.0% | |
| ! | GGCAAACACTGCTAGGTTGA+TGG | + | Chr4:78493686-78493705 | MsG0480022913.01.T01:CDS | 50.0% |
| ! | TGAGTTTGCCAAGGGAGTGT+GGG | + | Chr4:78493491-78493510 | MsG0480022913.01.T01:CDS | 50.0% |
| ! | AGTTTGCCAAGGGAGTGTGG+GGG | + | Chr4:78493493-78493512 | MsG0480022913.01.T01:CDS | 55.0% |
| ! | GAGTTTGCCAAGGGAGTGTG+GGG | + | Chr4:78493492-78493511 | MsG0480022913.01.T01:CDS | 55.0% |
| !! | CAAGGGAGTGTGGGGGTATT+GGG | + | Chr4:78493500-78493519 | MsG0480022913.01.T01:CDS | 55.0% |
| !! | TGTGGGGGTATTGGGAGCTA+GGG | + | Chr4:78493508-78493527 | MsG0480022913.01.T01:CDS | 55.0% |
| CCAATACCCCCACACTCCCT+TGG | - | Chr4:78493502-78493521 | None:intergenic | 60.0% | |
| ! | CCAAGGGAGTGTGGGGGTAT+TGG | + | Chr4:78493499-78493518 | MsG0480022913.01.T01:CDS | 60.0% |
| ! | GGTATTGGGAGCTAGGGGCA+TGG | + | Chr4:78493514-78493533 | MsG0480022913.01.T01:CDS | 60.0% |
| !! | GTGGGGGTATTGGGAGCTAG+GGG | + | Chr4:78493509-78493528 | MsG0480022913.01.T01:CDS | 60.0% |
| !! | GTGTGGGGGTATTGGGAGCT+AGG | + | Chr4:78493507-78493526 | MsG0480022913.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 78493402 | 78493791 | 78493402 | ID=MsG0480022913.01;Name=MsG0480022913.01 |
| Chr4 | mRNA | 78493402 | 78493791 | 78493402 | ID=MsG0480022913.01.T01;Parent=MsG0480022913.01;Name=MsG0480022913.01.T01;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|129 |
| Chr4 | exon | 78493402 | 78493791 | 78493402 | ID=MsG0480022913.01.T01:exon:23461;Parent=MsG0480022913.01.T01 |
| Chr4 | CDS | 78493402 | 78493791 | 78493402 | ID=MsG0480022913.01.T01:cds;Parent=MsG0480022913.01.T01 |
| Gene Sequence |
| Protein sequence |
