AlfalfaGEDB | MsG0780041224.01

M. sativa cultivar ZhongmuNo.1 / MsG0780041224.01

KEGG: K02917
GO: GO:0003735 GO:0005622 GO:0005840 GO:0006412
PFAM: PF01247

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041224.01.T01	GFY83575.1	66.667	81	26	1	1	80	1	81	2.01E-30	114

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041224.01.T01	Q9LMK0	82.456	57	10	0	1	57	1	57	6.15E-27	97.4

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041224.01.T01	A0A7J0EB01	66.667	81	26	1	1	80	1	81	9.58e-31	114

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048971.01	MsG0780041224.01	0.868389	6.770079e-66	2.444568e-62
MsG0180000139.01	MsG0780041224.01	0.865839	4.413512e-65	1.456525e-61
MsG0180000339.01	MsG0780041224.01	0.834492	2.904669e-56	3.452789e-53
MsG0180000575.01	MsG0780041224.01	0.905833	2.825722e-80	4.654158e-76
MsG0180000614.01	MsG0780041224.01	0.802374	5.784194e-49	2.828874e-46
MsG0180000743.01	MsG0780041224.01	0.862362	5.349704e-64	1.562487e-60
MsG0180003117.01	MsG0780041224.01	0.811782	5.894229e-51	3.681781e-48
MsG0180003651.01	MsG0780041224.01	0.842758	2.126844e-58	3.256788e-55
MsG0180003793.01	MsG0780041224.01	0.832564	8.796885e-56	9.869663e-53
MsG0180004129.01	MsG0780041224.01	0.815357	9.634574e-52	6.627851e-49
MsG0180004130.01	MsG0780041224.01	0.811746	5.999307e-51	3.743922e-48
MsG0180004481.01	MsG0780041224.01	0.842855	2.003786e-58	3.077877e-55
MsG0180004482.01	MsG0780041224.01	0.835686	1.452106e-56	1.789082e-53
MsG0180004509.01	MsG0780041224.01	0.878156	3.525908e-69	1.821491e-65
MsG0180004854.01	MsG0780041224.01	0.833997	3.865591e-56	4.527278e-53
MsG0180005426.01	MsG0780041224.01	0.817608	3.019239e-52	2.209010e-49
MsG0180005659.01	MsG0780041224.01	0.864364	1.282357e-64	4.015614e-61
MsG0280006424.01	MsG0780041224.01	0.801637	8.197521e-49	3.934422e-46
MsG0780040511.01	MsG0780041224.01	0.810628	1.048679e-50	6.353480e-48
MsG0780040782.01	MsG0780041224.01	0.870884	1.040991e-66	4.116273e-63
MsG0780040900.01	MsG0780041224.01	0.819044	1.428121e-52	1.087089e-49
MsG0780041048.01	MsG0780041224.01	0.820270	7.493456e-53	5.901698e-50
MsG0780041147.01	MsG0780041224.01	0.840204	1.001054e-57	1.415694e-54
MsG0780041223.01	MsG0780041224.01	0.832219	1.071043e-55	1.189305e-52
MsG0780041224.01	MsG0780041462.01	0.842585	2.364432e-58	3.601506e-55
MsG0780041224.01	MsG0880041967.01	0.868812	4.941419e-66	1.812015e-62
MsG0780041224.01	MsG0880042125.01	0.842390	2.662240e-58	4.030877e-55
MsG0780041224.01	MsG0880042131.01	0.812083	5.068246e-51	3.191709e-48
MsG0780041224.01	MsG0880044711.01	0.802221	6.217541e-49	3.028806e-46
MsG0780041224.01	MsG0880046575.01	0.802044	6.761068e-49	3.278784e-46
MsG0780041224.01	MsG0880046631.01	0.835546	1.575741e-56	1.933032e-53
MsG0780041224.01	MsG0880046879.01	0.849938	2.344281e-60	4.517643e-57
MsG0780041224.01	MsG0880047427.01	0.803029	4.236318e-49	2.106566e-46
MsG0280010776.01	MsG0780041224.01	0.819374	1.201086e-52	9.225476e-50
MsG0280010995.01	MsG0780041224.01	0.858534	7.712486e-63	1.974631e-59
MsG0280011234.01	MsG0780041224.01	0.819131	1.364562e-52	1.041118e-49
MsG0380012073.01	MsG0780041224.01	0.821730	3.456247e-53	2.835005e-50
MsG0680031488.01	MsG0780041224.01	0.830129	3.493249e-55	3.647701e-52
MsG0680031628.01	MsG0780041224.01	0.808114	3.630651e-50	2.058297e-47
MsG0680032770.01	MsG0780041224.01	0.844984	5.389350e-59	8.852137e-56
MsG0680032809.01	MsG0780041224.01	0.823543	1.309291e-53	1.130623e-50
MsG0680033699.01	MsG0780041224.01	0.827180	1.803307e-54	1.727940e-51
MsG0480018477.01	MsG0780041224.01	0.810930	9.020904e-51	5.510105e-48
MsG0480021006.01	MsG0780041224.01	0.806819	6.834424e-50	3.746080e-47
MsG0480021159.01	MsG0780041224.01	0.838932	2.143250e-57	2.914261e-54
MsG0480022836.01	MsG0780041224.01	0.816189	6.288077e-52	4.425418e-49
MsG0480022913.01	MsG0780041224.01	0.872694	2.611737e-67	1.103390e-63
MsG0480022960.01	MsG0780041224.01	0.822111	2.821816e-53	2.339794e-50
MsG0480023149.01	MsG0780041224.01	0.801233	9.919950e-49	4.712983e-46
MsG0480023221.01	MsG0780041224.01	0.832512	9.061977e-56	1.015132e-52
MsG0480023399.01	MsG0780041224.01	0.832320	1.011277e-55	1.126208e-52
MsG0480023467.01	MsG0780041224.01	0.818302	2.104307e-52	1.569220e-49
MsG0480023771.01	MsG0780041224.01	0.860359	2.182459e-63	5.949729e-60
MsG0480023844.01	MsG0780041224.01	0.820289	7.419114e-53	5.846115e-50
MsG0580024369.01	MsG0780041224.01	0.808559	2.918604e-50	1.674028e-47
MsG0580024754.01	MsG0780041224.01	0.812428	4.261285e-51	2.708809e-48
MsG0380015027.01	MsG0780041224.01	0.800146	1.652888e-48	7.639273e-46
MsG0380015435.01	MsG0780041224.01	0.819875	9.230146e-53	7.190098e-50
MsG0380016290.01	MsG0780041224.01	0.817060	4.010576e-52	2.890713e-49
MsG0380016559.01	MsG0780041224.01	0.805950	1.041958e-49	5.585608e-47
MsG0380016775.01	MsG0780041224.01	0.827285	1.702050e-54	1.635921e-51
MsG0380016778.01	MsG0780041224.01	0.810183	1.308496e-50	7.834392e-48
MsG0380016860.01	MsG0780041224.01	0.803149	4.000231e-49	1.995358e-46
MsG0380017382.01	MsG0780041224.01	0.886554	3.090509e-72	2.217869e-68
MsG0380017857.01	MsG0780041224.01	0.818206	2.212442e-52	1.645494e-49
MsG0580029132.01	MsG0780041224.01	0.829473	5.046889e-55	5.170483e-52
MsG0280006483.01	MsG0780041224.01	0.808986	2.365372e-50	1.372049e-47
MsG0280006819.01	MsG0780041224.01	0.800428	1.448178e-48	6.741553e-46
MsG0280006836.01	MsG0780041224.01	0.866267	3.230389e-65	1.082253e-61
MsG0280006993.01	MsG0780041224.01	0.875487	2.965732e-68	1.387039e-64
MsG0280007634.01	MsG0780041224.01	0.807408	5.130149e-50	2.855233e-47
MsG0280007787.01	MsG0780041224.01	0.804773	1.839184e-49	9.565402e-47
MsG0280009372.01	MsG0780041224.01	0.808406	3.145566e-50	1.797117e-47
MsG0280009375.01	MsG0780041224.01	0.832142	1.118949e-55	1.239679e-52
MsG0280009591.01	MsG0780041224.01	0.866382	2.970990e-65	9.993126e-62
MsG0280010383.01	MsG0780041224.01	0.821053	4.954392e-53	3.988060e-50
MsG0280010525.01	MsG0780041224.01	0.838434	2.881948e-57	3.859824e-54
MsG0780036546.01	MsG0780041224.01	0.810738	9.930048e-51	6.033882e-48
MsG0780036822.01	MsG0780041224.01	0.832800	7.689174e-56	8.687182e-53
MsG0780038400.01	MsG0780041224.01	0.852434	4.627030e-61	9.667619e-58
MsG0780038587.01	MsG0780041224.01	0.845861	3.117569e-59	5.267419e-56
MsG0780038909.01	MsG0780041224.01	0.807981	3.876106e-50	2.189692e-47
MsG0780039844.01	MsG0780041224.01	0.833763	4.424017e-56	5.144714e-53
MsG0780039848.01	MsG0780041224.01	0.802183	6.329852e-49	3.080517e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041224.01.T01	MTR_0699s0010	89.474	57	6	1	57	1	57	1.13e-30	104
MsG0780041224.01.T01	MTR_0699s0020	89.474	57	6	1	57	1	57	1.29e-30	104
MsG0780041224.01.T01	MTR_5g081705	85.965	57	8	1	57	26	82	3.49e-30	103
MsG0780041224.01.T01	MTR_5g081705	85.965	57	8	1	57	26	82	5.22e-30	103

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041224.01.T01	AT1G07070	82.456	57	10	1	57	1	57	6.27e-28	97.4
MsG0780041224.01.T01	AT1G74270	82.456	57	10	1	57	1	57	7.98e-28	97.4
MsG0780041224.01.T01	AT3G55750	78.571	56	12	2	57	1	56	5.23e-26	92.8
MsG0780041224.01.T01	AT1G41880	76.786	56	13	2	57	1	56	8.00e-26	92.0
MsG0780041224.01.T01	AT1G41880	76.786	56	13	2	57	1	56	8.00e-26	92.0

Find 54 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGTGTTCACTCCTTCAATT+TGG	0.242520	7:-88435798	None:intergenic
CTGTTTAGACTGTTCTTTGA+TGG	0.273778	7:+88437922	MsG0780041224.01.T01:three_prime_UTR
TCCATCTGCGTTTGAGTTGC+CGG	0.303207	7:+88435096	MsG0780041224.01.T01:five_prime_UTR
TGTTGCAGATTCAGGTGATT+AGG	0.325385	7:+88437826	MsG0780041224.01.T01:intron
GCCCCATATGCAGCGGTAAT+GGG	0.380708	7:-88435891	None:intergenic
CCAGAGATGTGTTTGGGTAC+TGG	0.398125	7:-88435774	None:intergenic
GAGTTTACCTCATATATTGC+TGG	0.402889	7:-88436100	None:intergenic
ATTTGGACCAGAGATGTGTT+TGG	0.405595	7:-88435781	None:intergenic
GAACCCATTACCGCTGCATA+TGG	0.409627	7:+88435888	MsG0780041224.01.T01:CDS
TGCCCCATATGCAGCGGTAA+TGG	0.410420	7:-88435892	None:intergenic
GACGTCCCTTCACCATCTTC+CGG	0.411385	7:-88435115	None:intergenic
GACGTCAAGGAGAGCGTGTC+AGG	0.412439	7:+88435132	MsG0780041224.01.T01:CDS
CCTGTCGGTTTGAGAGTGTT+TGG	0.414415	7:+88435068	MsG0780041224.01.T01:five_prime_UTR
TTTGGACCAGAGATGTGTTT+GGG	0.422500	7:-88435780	None:intergenic
CAGCACCATGTGTGTGGACT+TGG	0.449535	7:+88437987	MsG0780041224.01.T01:three_prime_UTR
GATGGAATGTTGTGTACTGC+TGG	0.453584	7:+88437940	MsG0780041224.01.T01:three_prime_UTR
AACCCATTACCGCTGCATAT+GGG	0.459805	7:+88435889	MsG0780041224.01.T01:CDS
ACATCTCTGGTCCAAATTGA+AGG	0.462436	7:+88435787	MsG0780041224.01.T01:CDS
TACGTGAGGGGTACAATCCT+TGG	0.470557	7:+88435655	MsG0780041224.01.T01:CDS
GGTTCCATTCTTCTTCACCT+TGG	0.492441	7:-88435870	None:intergenic
GTACAATCCTTGGATACAAA+AGG	0.498010	7:+88435665	MsG0780041224.01.T01:CDS
GAAGATGGTAGCTGGTATGC+TGG	0.512456	7:+88435823	MsG0780041224.01.T01:CDS
AAGGCCAAGGTGAAGAAGAA+TGG	0.517743	7:+88435866	MsG0780041224.01.T01:CDS
GTTTGGGTACTGGTTTACTT+TGG	0.518411	7:-88435764	None:intergenic
ATACCAGCTACCATCTTCCT+TGG	0.520678	7:-88435818	None:intergenic
GCGTTTGAGTTGCCGGAAGA+TGG	0.523180	7:+88435103	MsG0780041224.01.T01:exon
GCATATGGGGCAAGGTTACA+AGG	0.526923	7:+88435903	MsG0780041224.01.T01:CDS
CCAGTACCCAAACACATCTC+TGG	0.530530	7:+88435774	MsG0780041224.01.T01:CDS
TTGTATTAGGCTTTACGTGA+GGG	0.533599	7:+88435642	MsG0780041224.01.T01:intron
ACCCATTACCGCTGCATATG+GGG	0.539984	7:+88435890	MsG0780041224.01.T01:CDS
TTTGTATTAGGCTTTACGTG+AGG	0.553810	7:+88435641	MsG0780041224.01.T01:intron
TCCGGCAACTCAAACGCAGA+TGG	0.554512	7:-88435097	None:intergenic
AGTTTACCTCATATATTGCT+GGG	0.555512	7:-88436099	None:intergenic
AGCATATATCTACAAGGCCA+AGG	0.571107	7:+88435853	MsG0780041224.01.T01:CDS
GGAGTGAACACCAAGGAAGA+TGG	0.572693	7:+88435808	MsG0780041224.01.T01:CDS
ACACCAAGGAAGATGGTAGC+TGG	0.588664	7:+88435815	MsG0780041224.01.T01:CDS
GTACATAAATACTCTTACGC+GGG	0.591589	7:-88436077	None:intergenic
CCAAACACTCTCAAACCGAC+AGG	0.599002	7:-88435068	None:intergenic
TACAGTCAGCACCATGTGTG+TGG	0.604128	7:+88437981	MsG0780041224.01.T01:three_prime_UTR
TGTATTAGGCTTTACGTGAG+GGG	0.604275	7:+88435643	MsG0780041224.01.T01:intron
AGTTGCCGGAAGATGGTGAA+GGG	0.605695	7:+88435110	MsG0780041224.01.T01:exon
GGCAAGGTTACAAGGCCACA+TGG	0.606069	7:+88435911	MsG0780041224.01.T01:CDS
AAGATGGTGAAGGGACGTCA+AGG	0.612261	7:+88435119	MsG0780041224.01.T01:exon
TATGTACCCAGCAATATATG+AGG	0.621760	7:+88436093	MsG0780041224.01.T01:three_prime_UTR
TGATTAGGTCAGTAGAGTTG+CGG	0.623086	7:+88437841	MsG0780041224.01.T01:three_prime_UTR
AAGAAAAGCATATATCTACA+AGG	0.630318	7:+88435847	MsG0780041224.01.T01:CDS
GGTACATAAATACTCTTACG+CGG	0.639809	7:-88436078	None:intergenic
GATAACCAAGTCCACACACA+TGG	0.640711	7:-88437992	None:intergenic
TTACCGCTGCATATGGGGCA+AGG	0.645005	7:+88435895	MsG0780041224.01.T01:CDS
GAGTTGCCGGAAGATGGTGA+AGG	0.649061	7:+88435109	MsG0780041224.01.T01:exon
GAACAGTCTAAACAGAAGAA+CGG	0.650910	7:-88437914	None:intergenic
AAAATCCTAAAATCGCCTGT+CGG	0.675633	7:+88435053	MsG0780041224.01.T01:five_prime_UTR
TAACCTTGCCCCATATGCAG+CGG	0.677626	7:-88435898	None:intergenic
AATTGAAGGAGTGAACACCA+AGG	0.776962	7:+88435801	MsG0780041224.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTATGTTTAAAGAATTAA+TGG	-	Chr7:88436750-88436769	None:intergenic	10.0%
!!	AATTTAATTAAGGAAAATTT+AGG	+	Chr7:88437364-88437383	MsG0780041224.01.T01:intron	10.0%
!!	CAAAAAAAAAAAAAAAAAAC+AGG	-	Chr7:88436721-88436740	None:intergenic	10.0%
!!	TAAAGAATAAAAAGAATTAA+TGG	+	Chr7:88437389-88437408	MsG0780041224.01.T01:intron	10.0%
!!!	AATTTTGCAATTTATAAATA+GGG	+	Chr7:88437732-88437751	MsG0780041224.01.T01:intron	10.0%
!!!	ATATATATTAGCTTATTATA+TGG	-	Chr7:88435611-88435630	None:intergenic	10.0%
!!!	ATATTTATTTTAATGATAGT+AGG	-	Chr7:88435337-88435356	None:intergenic	10.0%
!!!	TAATTTTGCAATTTATAAAT+AGG	+	Chr7:88437731-88437750	MsG0780041224.01.T01:intron	10.0%
!!!	TTTTTTTTTAATCATCATTT+GGG	+	Chr7:88436008-88436027	MsG0780041224.01.T01:intron	10.0%
!!!	TTTTTTTTTTAATCATCATT+TGG	+	Chr7:88436007-88436026	MsG0780041224.01.T01:intron	10.0%
!!	ATTATGTTTAATTCCTTAAA+AGG	+	Chr7:88437329-88437348	MsG0780041224.01.T01:intron	15.0%
!!!	AAACATGAAAATTTTCAAAT+TGG	-	Chr7:88435521-88435540	None:intergenic	15.0%
!!!	ATCATTGTTTTTAATTCATT+TGG	+	Chr7:88435280-88435299	MsG0780041224.01.T01:intron	15.0%
!!!	ATTTTTGTTTCATTTGTATT+AGG	+	Chr7:88435629-88435648	MsG0780041224.01.T01:intron	15.0%
!!!	GATCTCATTTTTTTTATTTA+TGG	-	Chr7:88436938-88436957	None:intergenic	15.0%
!!!	TATCAATAATACCTTTTTTT+TGG	-	Chr7:88437043-88437062	None:intergenic	15.0%
!!!	TTAATTATTACCATTGATTT+GGG	-	Chr7:88435978-88435997	None:intergenic	15.0%
!!!	TTAATTCTTTAATCCTTTTA+AGG	-	Chr7:88437345-88437364	None:intergenic	15.0%
!!!	TTTTATTGAATCATCATTTT+AGG	+	Chr7:88437218-88437237	MsG0780041224.01.T01:intron	15.0%
!!!	TTTTTTTAAGAATAGAAAAG+TGG	+	Chr7:88437007-88437026	MsG0780041224.01.T01:intron	15.0%
!!	AAAAAAAAAAAACAGGTTTG+AGG	+	Chr7:88437424-88437443	MsG0780041224.01.T01:intron	20.0%
!!	AAAAAAAAAAAACAGGTTTG+AGG	-	Chr7:88437443-88437424	None:intergenic	20.0%
!!	AAAAAGAATCGTGTATTTAA+TGG	-	Chr7:88436880-88436899	None:intergenic	20.0%
!!	AAATCCATATTTCTTCATTA+TGG	-	Chr7:88436852-88436871	None:intergenic	20.0%
!!	ACGTTTCTTAATTAAAAAGA+GGG	+	Chr7:88436980-88436999	MsG0780041224.01.T01:intron	20.0%
!!	GATTGAATATGTAGTATTAT+AGG	-	Chr7:88436960-88436979	None:intergenic	20.0%
!!	GTCAACAAAAAAAAAAAAAC+AGG	+	Chr7:88437417-88437436	MsG0780041224.01.T01:intron	20.0%
!!	GTTTATTTGTACCAAAAAAA+AGG	+	Chr7:88437029-88437048	MsG0780041224.01.T01:intron	20.0%
!!	TATCTAATATGAGAACTATA+TGG	+	Chr7:88436468-88436487	MsG0780041224.01.T01:intron	20.0%
!!	TGCTTACATATTTACAAATA+TGG	+	Chr7:88436258-88436277	MsG0780041224.01.T01:intron	20.0%
!!!	AATTGTTTGTCTTTTCTTTT+TGG	+	Chr7:88436140-88436159	MsG0780041224.01.T01:intron	20.0%
!!!	GTTAATTATTACCATTGATT+TGG	-	Chr7:88435979-88435998	None:intergenic	20.0%
!!!	TAATTATTACCATTGATTTG+GGG	-	Chr7:88435977-88435996	None:intergenic	20.0%
!	ACTATATGGTTTCTAAATCA+TGG	+	Chr7:88436482-88436501	MsG0780041224.01.T01:intron	25.0%
!	CACGTTTCTTAATTAAAAAG+AGG	+	Chr7:88436979-88436998	MsG0780041224.01.T01:intron	25.0%
!	CTCTATGATAACATAGTTTA+TGG	+	Chr7:88437621-88437640	MsG0780041224.01.T01:intron	25.0%
!	TAGTTTACAGAGTAAAGTAA+AGG	+	Chr7:88435397-88435416	MsG0780041224.01.T01:intron	25.0%
!	TTTATCTATCATGATTCATG+AGG	+	Chr7:88436403-88436422	MsG0780041224.01.T01:intron	25.0%
!!	AAGAAAAGCATATATCTACA+AGG	+	Chr7:88435847-88435866	MsG0780041224.01.T01:CDS	25.0%
!!	ACTTTTCTAATGAAAATGTC+TGG	+	Chr7:88435691-88435710	MsG0780041224.01.T01:intron	25.0%
!!	TATCAGACACTTATATTTTG+TGG	+	Chr7:88435493-88435512	MsG0780041224.01.T01:intron	25.0%
!!	TTTTCTATCATAGATCAGAT+AGG	-	Chr7:88436521-88436540	None:intergenic	25.0%
!!!	AATTATTACCATTGATTTGG+GGG	-	Chr7:88435976-88435995	None:intergenic	25.0%
!!!	TTTTTTTTTGTTGCAGATTC+AGG	+	Chr7:88437818-88437837	MsG0780041224.01.T01:intron	25.0%
	AAATATGTAAGCACGTAAAC+AGG	-	Chr7:88436251-88436270	None:intergenic	30.0%
	AAGAATTGATGTCTACATGA+AGG	-	Chr7:88436356-88436375	None:intergenic	30.0%
	AGTTTACCTCATATATTGCT+GGG	-	Chr7:88436102-88436121	None:intergenic	30.0%
	ATCTGAAAATCAACTGTATC+TGG	-	Chr7:88435470-88435489	None:intergenic	30.0%
	CTCTCGTATTTCTTCATTTA+TGG	+	Chr7:88437273-88437292	MsG0780041224.01.T01:intron	30.0%
	TACTTTCTCTTGATGTTACT+TGG	+	Chr7:88437794-88437813	MsG0780041224.01.T01:intron	30.0%
	TCGTGTTTGGATTAATTTCA+GGG	+	Chr7:88436052-88436071	MsG0780041224.01.T01:intron	30.0%
	TGTGCCATAATGAAGAAATA+TGG	+	Chr7:88436845-88436864	MsG0780041224.01.T01:intron	30.0%
	TGTTATCTTAATCTCGTGTT+TGG	+	Chr7:88436039-88436058	MsG0780041224.01.T01:intron	30.0%
!	AAAAGACGTTTTTATCCCTT+CGG	-	Chr7:88437558-88437577	None:intergenic	30.0%
!	AAGCATTTTCTCATTTGATG+GGG	+	Chr7:88436205-88436224	MsG0780041224.01.T01:intron	30.0%
!	AAGTTTACCTTTTGTATCCA+AGG	-	Chr7:88435675-88435694	None:intergenic	30.0%
!	CAAGCATTTTCTCATTTGAT+GGG	+	Chr7:88436204-88436223	MsG0780041224.01.T01:intron	30.0%
!!	CAAACAATTCAAAAGCATCT+AGG	-	Chr7:88436129-88436148	None:intergenic	30.0%
!!	TTCTTATGCTCTAGAGTTAT+GGG	+	Chr7:88436426-88436445	MsG0780041224.01.T01:intron	30.0%
	AAAATCCTAAAATCGCCTGT+CGG	+	Chr7:88435053-88435072	MsG0780041224.01.T01:five_prime_UTR	35.0%
	AAATGTCTGGTTAGATGCTT+TGG	+	Chr7:88435704-88435723	MsG0780041224.01.T01:intron	35.0%
	CATCTGCAATCAGTAGTTAA+TGG	-	Chr7:88437245-88437264	None:intergenic	35.0%
	CCAAGGAAATCTGAAAACTT+CGG	-	Chr7:88437469-88437488	None:intergenic	35.0%
	CCATCAAATGAGAAAATGCT+TGG	-	Chr7:88436206-88436225	None:intergenic	35.0%
	CTCGTGTTTGGATTAATTTC+AGG	+	Chr7:88436051-88436070	MsG0780041224.01.T01:intron	35.0%
	CTGTTTAGACTGTTCTTTGA+TGG	+	Chr7:88437922-88437941	MsG0780041224.01.T01:three_prime_UTR	35.0%
	GAAAATCAACTGTATCTGGT+TGG	-	Chr7:88435466-88435485	None:intergenic	35.0%
	GAACAGTCTAAACAGAAGAA+CGG	-	Chr7:88437917-88437936	None:intergenic	35.0%
	GAGTTTACCTCATATATTGC+TGG	-	Chr7:88436103-88436122	None:intergenic	35.0%
	GGTACATAAATACTCTTACG+CGG	-	Chr7:88436081-88436100	None:intergenic	35.0%
	GTACAATCCTTGGATACAAA+AGG	+	Chr7:88435665-88435684	MsG0780041224.01.T01:CDS	35.0%
	GTACATAAATACTCTTACGC+GGG	-	Chr7:88436080-88436099	None:intergenic	35.0%
	TAACTGCCGAATTATGAAAC+CGG	+	Chr7:88436607-88436626	MsG0780041224.01.T01:intron	35.0%
	TAACTGCCGAATTATGAAAC+CGG	-	Chr7:88436626-88436607	None:intergenic	35.0%
	TATGTACCCAGCAATATATG+AGG	+	Chr7:88436093-88436112	MsG0780041224.01.T01:three_prime_UTR	35.0%
	TTAACTACCGAGGAAAACTT+CGG	+	Chr7:88436636-88436655	MsG0780041224.01.T01:intron	35.0%
	TTAACTACCGAGGAAAACTT+CGG	-	Chr7:88436655-88436636	None:intergenic	35.0%
	TTGTATTAGGCTTTACGTGA+GGG	+	Chr7:88435642-88435661	MsG0780041224.01.T01:intron	35.0%
	TTTGTATTAGGCTTTACGTG+AGG	+	Chr7:88435641-88435660	MsG0780041224.01.T01:intron	35.0%
!	AAAAGACGTTTTTACCCCTT+CGG	+	Chr7:88436580-88436599	MsG0780041224.01.T01:intron	35.0%
!	CCAAGCATTTTCTCATTTGA+TGG	+	Chr7:88436203-88436222	MsG0780041224.01.T01:intron	35.0%
!!	GTTCTTATGCTCTAGAGTTA+TGG	+	Chr7:88436425-88436444	MsG0780041224.01.T01:intron	35.0%
!!!	GGATGATTTTTAGTAGCTCA+AGG	+	Chr7:88437862-88437881	MsG0780041224.01.T01:three_prime_UTR	35.0%
	AATTGAAGGAGTGAACACCA+AGG	+	Chr7:88435801-88435820	MsG0780041224.01.T01:CDS	40.0%
	ACAATACCACTGTTACCATG+TGG	-	Chr7:88435929-88435948	None:intergenic	40.0%
	ACAGGTCAGAAGTTTCAAAC+AGG	-	Chr7:88436233-88436252	None:intergenic	40.0%
	ACATCTCTGGTCCAAATTGA+AGG	+	Chr7:88435787-88435806	MsG0780041224.01.T01:CDS	40.0%
	AGCATATATCTACAAGGCCA+AGG	+	Chr7:88435853-88435872	MsG0780041224.01.T01:CDS	40.0%
	ATTTGGACCAGAGATGTGTT+TGG	-	Chr7:88435784-88435803	None:intergenic	40.0%
	CCGAGGAAATCTGAAAACTT+CGG	+	Chr7:88436669-88436688	MsG0780041224.01.T01:intron	40.0%
	TATAAATAGGGCCTCTTCTC+TGG	+	Chr7:88437744-88437763	MsG0780041224.01.T01:intron	40.0%
	TCAAATCTCCCCCAAATCAA+TGG	+	Chr7:88435965-88435984	MsG0780041224.01.T01:intron	40.0%
	TGATTAGGTCAGTAGAGTTG+CGG	+	Chr7:88437841-88437860	MsG0780041224.01.T01:three_prime_UTR	40.0%
	TGGTGTTCACTCCTTCAATT+TGG	-	Chr7:88435801-88435820	None:intergenic	40.0%
	TGTATTAGGCTTTACGTGAG+GGG	+	Chr7:88435643-88435662	MsG0780041224.01.T01:intron	40.0%
	TGTTGCAGATTCAGGTGATT+AGG	+	Chr7:88437826-88437845	MsG0780041224.01.T01:intron	40.0%
	TTTGGACCAGAGATGTGTTT+GGG	-	Chr7:88435783-88435802	None:intergenic	40.0%
!	AAACAGGTTTGAGGTTTCCT+CGG	+	Chr7:88437433-88437452	MsG0780041224.01.T01:intron	40.0%
!	AAACAGGTTTGAGGTTTCCT+CGG	-	Chr7:88437452-88437433	None:intergenic	40.0%
!	CCGAAGTTTTCAGATTTCCT+CGG	-	Chr7:88437485-88437466	None:intergenic	40.0%
!	CCGAAGTTTTCAGATTTCCT+TGG	+	Chr7:88437466-88437485	MsG0780041224.01.T01:intron	40.0%
!	CTGTAGCCGGTTTCATAATT+CGG	+	Chr7:88437522-88437541	MsG0780041224.01.T01:intron	40.0%
!	TGCTGTTGACACATTAGCTT+TGG	+	Chr7:88436542-88436561	MsG0780041224.01.T01:intron	40.0%
!	TGCTGTTGACACATTAGCTT+TGG	-	Chr7:88436561-88436542	None:intergenic	40.0%
!	TTAACTGCCGAAGTTTTCCT+CGG	+	Chr7:88437492-88437511	MsG0780041224.01.T01:intron	40.0%
!	TTAACTGCCGAAGTTTTCCT+CGG	-	Chr7:88437511-88437492	None:intergenic	40.0%
!!	GGGGAGATTTGACTTGAATT+TGG	-	Chr7:88435957-88435976	None:intergenic	40.0%
!!	GTTTGGGTACTGGTTTACTT+TGG	-	Chr7:88435767-88435786	None:intergenic	40.0%
!!	TTGACACATTAGCTTTGGCT+AGG	+	Chr7:88436547-88436566	MsG0780041224.01.T01:intron	40.0%
!!	TTGACACATTAGCTTTGGCT+AGG	-	Chr7:88436566-88436547	None:intergenic	40.0%
	AACCCATTACCGCTGCATAT+GGG	+	Chr7:88435889-88435908	MsG0780041224.01.T01:CDS	45.0%
	AAGGCCAAGGTGAAGAAGAA+TGG	+	Chr7:88435866-88435885	MsG0780041224.01.T01:CDS	45.0%
	ATACCAGCTACCATCTTCCT+TGG	-	Chr7:88435821-88435840	None:intergenic	45.0%
	ATTCGGCAGTTAACTACCGA+AGG	+	Chr7:88437539-88437558	MsG0780041224.01.T01:intron	45.0%
	ATTCGGCAGTTAACTACCGA+AGG	-	Chr7:88437558-88437539	None:intergenic	45.0%
	GATAACCAAGTCCACACACA+TGG	-	Chr7:88437995-88438014	None:intergenic	45.0%
	GATGGAATGTTGTGTACTGC+TGG	+	Chr7:88437940-88437959	MsG0780041224.01.T01:three_prime_UTR	45.0%
	GGTTCCATTCTTCTTCACCT+TGG	-	Chr7:88435873-88435892	None:intergenic	45.0%
	TTCGGCAGTTAACTACCGAA+GGG	+	Chr7:88437540-88437559	MsG0780041224.01.T01:intron	45.0%
	TTCGGCAGTTAACTACCGAA+GGG	-	Chr7:88437559-88437540	None:intergenic	45.0%
!	CTGCAGCCGGTTTCATAATT+CGG	-	Chr7:88436616-88436635	None:intergenic	45.0%
!	GTGTGTGGACTTGGTTATCT+GGG	+	Chr7:88437996-88438015	MsG0780041224.01.T01:three_prime_UTR	45.0%
!	TCAAACCGACAGGCGATTTT+AGG	-	Chr7:88435061-88435080	None:intergenic	45.0%
!	TGTGTGTGGACTTGGTTATC+TGG	+	Chr7:88437995-88438014	MsG0780041224.01.T01:three_prime_UTR	45.0%
!!	ATTAAAGAATTAATTTAATA+AGG	-	Chr7:88436775-88436794	None:intergenic	5.0%
!!	TAAAGAATTAAATTTAATTA+AGG	+	Chr7:88437354-88437373	MsG0780041224.01.T01:intron	5.0%
!!!	TTAATTCTTTAATTATTTTA+AGG	+	Chr7:88436782-88436801	MsG0780041224.01.T01:intron	5.0%
	AAGATGGTGAAGGGACGTCA+AGG	+	Chr7:88435119-88435138	MsG0780041224.01.T01:exon	50.0%
	ACAAGGCCACATGGTAACAG+TGG	+	Chr7:88435920-88435939	MsG0780041224.01.T01:intron	50.0%
	ACACCAAGGAAGATGGTAGC+TGG	+	Chr7:88435815-88435834	MsG0780041224.01.T01:CDS	50.0%
	ACCCATTACCGCTGCATATG+GGG	+	Chr7:88435890-88435909	MsG0780041224.01.T01:CDS	50.0%
	ACTTCGGCAGTTAACTGCCA+AGG	-	Chr7:88437486-88437505	None:intergenic	50.0%
	AGTTGCCGGAAGATGGTGAA+GGG	+	Chr7:88435110-88435129	MsG0780041224.01.T01:exon	50.0%
	CCAAACACTCTCAAACCGAC+AGG	-	Chr7:88435071-88435090	None:intergenic	50.0%
	CCAGAGATGTGTTTGGGTAC+TGG	-	Chr7:88435777-88435796	None:intergenic	50.0%
	CCAGTACCCAAACACATCTC+TGG	+	Chr7:88435774-88435793	MsG0780041224.01.T01:CDS	50.0%
	CCTCGGTAGTTAACTGTAGC+CGG	+	Chr7:88437509-88437528	MsG0780041224.01.T01:intron	50.0%
	GAACCCATTACCGCTGCATA+TGG	+	Chr7:88435888-88435907	MsG0780041224.01.T01:CDS	50.0%
	GAAGATGGTAGCTGGTATGC+TGG	+	Chr7:88435823-88435842	MsG0780041224.01.T01:CDS	50.0%
	GCATATGGGGCAAGGTTACA+AGG	+	Chr7:88435903-88435922	MsG0780041224.01.T01:CDS	50.0%
	GGAGTGAACACCAAGGAAGA+TGG	+	Chr7:88435808-88435827	MsG0780041224.01.T01:CDS	50.0%
	TAACCTTGCCCCATATGCAG+CGG	-	Chr7:88435901-88435920	None:intergenic	50.0%
	TACAGTCAGCACCATGTGTG+TGG	+	Chr7:88437981-88438000	MsG0780041224.01.T01:three_prime_UTR	50.0%
	TCGGCAGTTAACTACCGAAG+GGG	-	Chr7:88437560-88437541	None:intergenic	50.0%
!	TACGTGAGGGGTACAATCCT+TGG	+	Chr7:88435655-88435674	MsG0780041224.01.T01:CDS	50.0%
!!	CCTGTCGGTTTGAGAGTGTT+TGG	+	Chr7:88435068-88435087	MsG0780041224.01.T01:five_prime_UTR	50.0%
!!	TCCATCTGCGTTTGAGTTGC+CGG	+	Chr7:88435096-88435115	MsG0780041224.01.T01:five_prime_UTR	50.0%
	ACTTCGGCAGTTAACTGCCG+AGG	+	Chr7:88436652-88436671	MsG0780041224.01.T01:intron	55.0%
	ACTTCGGCAGTTAACTGCCG+AGG	-	Chr7:88436671-88436652	None:intergenic	55.0%
	CAGCACCATGTGTGTGGACT+TGG	+	Chr7:88437987-88438006	MsG0780041224.01.T01:three_prime_UTR	55.0%
	CCGGCTACAGTTAACTACCG+AGG	-	Chr7:88437512-88437531	None:intergenic	55.0%
	CCTCGGTAGTTAACTGCAGC+CGG	-	Chr7:88436629-88436648	None:intergenic	55.0%
	GACGTCCCTTCACCATCTTC+CGG	-	Chr7:88435118-88435137	None:intergenic	55.0%
	GAGTTGCCGGAAGATGGTGA+AGG	+	Chr7:88435109-88435128	MsG0780041224.01.T01:exon	55.0%
	GCCCCATATGCAGCGGTAAT+GGG	-	Chr7:88435894-88435913	None:intergenic	55.0%
	GGCAAGGTTACAAGGCCACA+TGG	+	Chr7:88435911-88435930	MsG0780041224.01.T01:CDS	55.0%
	TCCGGCAACTCAAACGCAGA+TGG	-	Chr7:88435100-88435119	None:intergenic	55.0%
	TGCCCCATATGCAGCGGTAA+TGG	-	Chr7:88435895-88435914	None:intergenic	55.0%
	TTACCGCTGCATATGGGGCA+AGG	+	Chr7:88435895-88435914	MsG0780041224.01.T01:CDS	55.0%
!	GTGCTGCTAGACCAGAGAAG+AGG	-	Chr7:88437758-88437777	None:intergenic	55.0%
!!	GCGTTTGAGTTGCCGGAAGA+TGG	+	Chr7:88435103-88435122	MsG0780041224.01.T01:exon	55.0%
	CCGGCTGCAGTTAACTACCG+AGG	+	Chr7:88436626-88436645	MsG0780041224.01.T01:intron	60.0%
	CTCTGGTCTAGCAGCACGCA+TGG	+	Chr7:88437761-88437780	MsG0780041224.01.T01:intron	60.0%
!	GACGTCAAGGAGAGCGTGTC+AGG	+	Chr7:88435132-88435151	MsG0780041224.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	88435044	88438015	88435044	ID=MsG0780041224.01;Name=MsG0780041224.01
Chr7	mRNA	88435044	88438015	88435044	ID=MsG0780041224.01.T01;Parent=MsG0780041224.01;Name=MsG0780041224.01.T01;_AED=0.47;_eAED=0.47;_QI=78\|0.75\|0.8\|1\|0.5\|0.4\|5\|211\|83
Chr7	exon	88435044	88435153	88435044	ID=MsG0780041224.01.T01:exon:41498;Parent=MsG0780041224.01.T01
Chr7	exon	88435651	88435686	88435651	ID=MsG0780041224.01.T01:exon:41499;Parent=MsG0780041224.01.T01
Chr7	exon	88435762	88435932	88435762	ID=MsG0780041224.01.T01:exon:41500;Parent=MsG0780041224.01.T01
Chr7	exon	88436073	88436114	88436073	ID=MsG0780041224.01.T01:exon:41501;Parent=MsG0780041224.01.T01
Chr7	exon	88437834	88438015	88437834	ID=MsG0780041224.01.T01:exon:41502;Parent=MsG0780041224.01.T01
Chr7	five_prime_UTR	88435044	88435121	88435044	ID=MsG0780041224.01.T01:five_prime_utr;Parent=MsG0780041224.01.T01
Chr7	CDS	88435122	88435153	88435122	ID=MsG0780041224.01.T01:cds;Parent=MsG0780041224.01.T01
Chr7	CDS	88435651	88435686	88435651	ID=MsG0780041224.01.T01:cds;Parent=MsG0780041224.01.T01
Chr7	CDS	88435762	88435932	88435762	ID=MsG0780041224.01.T01:cds;Parent=MsG0780041224.01.T01
Chr7	CDS	88436073	88436085	88436073	ID=MsG0780041224.01.T01:cds;Parent=MsG0780041224.01.T01
Chr7	three_prime_UTR	88436086	88436114	88436086	ID=MsG0780041224.01.T01:three_prime_utr;Parent=MsG0780041224.01.T01
Chr7	three_prime_UTR	88437834	88438015	88437834	ID=MsG0780041224.01.T01:three_prime_utr;Parent=MsG0780041224.01.T01

Gene Sequence	>MsG0780041224.01.T01 ATGGTGAAGGGACGTCAAGGAGAGCGTGTCAGGCTTTACGTGAGGGGTACAATCCTTGGATACAAAAGATCCAAAGTAAACCAGTACCCAAACACATCTCTGGTCCAAATTGAAGGAGTGAACACCAAGGAAGATGGTAGCTGGTATGCTGGCAAGAAAAGCATATATCTACAAGGCCAAGGTGAAGAAGAATGGAACCCATTACCGCTGCATATGGGGCAAGGTTACAAGGCCACATGGGTTCCCGCGTAA
Protein sequence	>MsG0780041224.01.T01 MVKGRQGERVRLYVRGTILGYKRSKVNQYPNTSLVQIEGVNTKEDGSWYAGKKSIYLQGQGEEEWNPLPLHMGQGYKATWVPA*