AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005426.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005426.01.T01 MTR_1g100960 100.000 111 0 0 29 139 1 111 1.07e-74 218
MsG0180005426.01.T01 MTR_6g053730 96.396 111 4 0 29 139 1 111 5.56e-72 211
MsG0180005426.01.T01 MTR_1g100960 100.000 101 0 0 29 129 1 101 1.06e-67 200
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005426.01.T01 AT3G53740 80.180 111 22 0 29 139 1 111 7.58e-58 175
MsG0180005426.01.T01 AT3G53740 80.180 111 22 0 29 139 1 111 7.58e-58 175
MsG0180005426.01.T01 AT3G53740 80.180 111 22 0 29 139 1 111 7.58e-58 175
MsG0180005426.01.T01 AT2G37600 82.692 104 18 0 36 139 8 111 5.93e-56 171
MsG0180005426.01.T01 AT2G37600 82.692 104 18 0 36 139 8 111 5.93e-56 171
MsG0180005426.01.T01 AT5G02450 83.019 106 17 1 35 139 3 108 1.23e-55 169
MsG0180005426.01.T01 AT3G53740 72.072 111 22 1 29 139 1 102 1.43e-48 151

Find 50 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCGCACTCTCTCTGGTGTT+AGG 0.277881 1:+91712318 MsG0180005426.01.T01:CDS
AAACCAGTACTTGGTTGTTT+AGG 0.306770 1:-91712482 None:intergenic
CTTCTTCTTTGCACGTTTGT+GGG 0.313110 1:-91713142 None:intergenic
TCTTCTTCTTTGCACGTTTG+TGG 0.365751 1:-91713143 None:intergenic
TTTATTTGTGACAGGAGAAA+TGG 0.397262 1:+91712457 MsG0180005426.01.T01:intron
AAAAGACAAGAGGGCACTTA+AGG 0.398330 1:+91713098 MsG0180005426.01.T01:CDS
CCGCACTCTCTCTGGTGTTA+GGG 0.410246 1:+91712319 MsG0180005426.01.T01:CDS
CCATTCCACCGCACTCTCTC+TGG 0.423239 1:+91712311 MsG0180005426.01.T01:CDS
TCCACGTCCTTCAGCTCGCA+AGG 0.427898 1:+91712553 MsG0180005426.01.T01:CDS
TGCAGGGCTGGTGGTGGAGC+AGG 0.430321 1:+91713313 MsG0180005426.01.T01:intron
AGCAGGAGATAAGAAGAAAT+AGG 0.434078 1:+91713330 MsG0180005426.01.T01:CDS
CATCGTCACAAAGAAGGAAT+TGG 0.434230 1:+91712529 MsG0180005426.01.T01:CDS
GAAGTGAAGAAGCTAACCTT+AGG 0.445240 1:-91712289 None:intergenic
CCAAGTACTGGTTTGTTCGT+TGG 0.458873 1:+91712491 MsG0180005426.01.T01:CDS
AAGGTTGCAAAGAGAAAGCT+TGG 0.461674 1:+91713117 MsG0180005426.01.T01:CDS
CCAACGAACAAACCAGTACT+TGG 0.464574 1:-91712491 None:intergenic
CCAGAGAGAGTGCGGTGGAA+TGG 0.480153 1:-91712311 None:intergenic
AACCTCATTAGAGAGGTTGC+TGG 0.487721 1:+91713030 MsG0180005426.01.T01:CDS
ATCACTGAGTTGCTCAAGGT+TGG 0.503868 1:+91713075 MsG0180005426.01.T01:CDS
TCAATGTGCAGGGCTGGTGG+TGG 0.508415 1:+91713307 MsG0180005426.01.T01:intron
AGGAGATGTCTAATGTCCTC+AGG 0.510350 1:+91713169 MsG0180005426.01.T01:CDS
ACCTCATTAGAGAGGTTGCT+GGG 0.511316 1:+91713031 MsG0180005426.01.T01:CDS
CCCTTGCGAGCTGAAGGACG+TGG 0.511446 1:-91712554 None:intergenic
TCTGAGATCACCGTCACTAC+AGG 0.512556 1:+91712344 MsG0180005426.01.T01:CDS
TTTGTTATGGCTTCACATGA+TGG 0.517974 1:+91713364 MsG0180005426.01.T01:three_prime_UTR
CCACGTCCTTCAGCTCGCAA+GGG 0.521414 1:+91712554 MsG0180005426.01.T01:CDS
TTGTTATGGCTTCACATGAT+GGG 0.522554 1:+91713365 MsG0180005426.01.T01:three_prime_UTR
AGGCCACATCGTCACAAAGA+AGG 0.551215 1:+91712523 MsG0180005426.01.T01:CDS
GCTCCTAAACAACCAAGTAC+TGG 0.551611 1:+91712479 MsG0180005426.01.T01:CDS
CACGTCCTTCAGCTCGCAAG+GGG 0.561496 1:+91712555 MsG0180005426.01.T01:CDS
ATTCCTTCTTTGTGACGATG+TGG 0.577252 1:-91712526 None:intergenic
TTGTTCGTTGGATTGAACAA+AGG 0.583526 1:+91712503 MsG0180005426.01.T01:CDS
TCAGTGATACGCTTCTCGTA+AGG 0.584616 1:-91713060 None:intergenic
AAGTGAAGAAGCTAACCTTA+GGG 0.585753 1:-91712288 None:intergenic
ACCCAGCAACCTCTCTAATG+AGG 0.601885 1:-91713032 None:intergenic
TGTGAGGAACCTCATTAGAG+AGG 0.602916 1:+91713023 MsG0180005426.01.T01:CDS
TCGTTCAGAAAACAAGCAAG+AGG 0.606732 1:+91712992 MsG0180005426.01.T01:intron
CCCTAACACCAGAGAGAGTG+CGG 0.614155 1:-91712319 None:intergenic
ACGTGCAAAGAAGAAGAGAG+AGG 0.618868 1:+91713149 MsG0180005426.01.T01:CDS
GCAAGAGGGTGCACTTTGTG+AGG 0.622900 1:+91713007 MsG0180005426.01.T01:CDS
GAAATGAATACCTGTAGTGA+CGG 0.626819 1:-91712354 None:intergenic
CTAATGTCCTCAGGAAGATG+AGG 0.627117 1:+91713178 MsG0180005426.01.T01:CDS
CAAGGTTGGAAAAGACAAGA+GGG 0.628294 1:+91713089 MsG0180005426.01.T01:CDS
GCGTATCACTGAGTTGCTCA+AGG 0.629950 1:+91713071 MsG0180005426.01.T01:CDS
CGTTCAGAAAACAAGCAAGA+GGG 0.632672 1:+91712993 MsG0180005426.01.T01:intron
AGATCCTCGAAAGAACCCTA+AGG 0.650922 1:+91712273 None:intergenic
TCAAGGTTGGAAAAGACAAG+AGG 0.663036 1:+91713088 MsG0180005426.01.T01:CDS
GAACAAACCTCATCTTCCTG+AGG 0.694769 1:-91713185 None:intergenic
ACGTCCTTCAGCTCGCAAGG+GGG 0.706112 1:+91712556 MsG0180005426.01.T01:CDS
TAACACCAGAGAGAGTGCGG+TGG 0.731080 1:-91712316 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTAAATCTAACATAAAA+TGG - Chr1:91712618-91712637 None:intergenic 10.0%
!!! ATTTTATGTTAGATTTAAAT+TGG + Chr1:91712617-91712636 MsG0180005426.01.T01:intron 10.0%
!!! TAGATATCAAAAGATTTTAT+GGG + Chr1:91713528-91713547 MsG0180005426.01.T01:three_prime_UTR 15.0%
!!! TTTATGGGTTTATATTTTTA+AGG + Chr1:91713543-91713562 MsG0180005426.01.T01:three_prime_UTR 15.0%
!! AACGAATAAAAACAATCTTT+AGG - Chr1:91712977-91712996 None:intergenic 20.0%
!! ACGAATAAAAACAATCTTTA+GGG - Chr1:91712976-91712995 None:intergenic 20.0%
!!! AAATTGGAATTATTAGCTTA+AGG + Chr1:91712633-91712652 MsG0180005426.01.T01:intron 20.0%
!!! CTAGATATCAAAAGATTTTA+TGG + Chr1:91713527-91713546 MsG0180005426.01.T01:three_prime_UTR 20.0%
!!! TTTGTTATTATTGTTATGCT+TGG + Chr1:91713215-91713234 MsG0180005426.01.T01:intron 20.0%
! AATAGAAGGAGAAATAATGT+TGG + Chr1:91712858-91712877 MsG0180005426.01.T01:intron 25.0%
! ATAGAAGGAGAAATAATGTT+GGG + Chr1:91712859-91712878 MsG0180005426.01.T01:intron 25.0%
! ATTAATTCATAAGCTTGTCA+AGG + Chr1:91712766-91712785 MsG0180005426.01.T01:intron 25.0%
! CATTATTTCATTCATTCCTA+TGG - Chr1:91712907-91712926 None:intergenic 25.0%
! CGAATAAAAACAATCTTTAG+GGG - Chr1:91712975-91712994 None:intergenic 25.0%
! GTTATTATGATAAACATTCC+CGG + Chr1:91713244-91713263 MsG0180005426.01.T01:intron 25.0%
! TTAAGAAAAAACTGCTTATG+AGG + Chr1:91712793-91712812 MsG0180005426.01.T01:intron 25.0%
! TTATGAGGATGATATTTATG+AGG + Chr1:91712808-91712827 MsG0180005426.01.T01:intron 25.0%
! TTATTGTTATGCTTGGTATT+TGG + Chr1:91713222-91713241 MsG0180005426.01.T01:intron 25.0%
! TTTGTTTGTTTATTTGTGAC+AGG + Chr1:91712449-91712468 MsG0180005426.01.T01:intron 25.0%
!! TTAGATATTTTCAATGTGCA+GGG + Chr1:91713297-91713316 MsG0180005426.01.T01:intron 25.0%
!! TTTAGATATTTTCAATGTGC+AGG + Chr1:91713296-91713315 MsG0180005426.01.T01:intron 25.0%
!!! GGTTTTGTTTACTTTTGTTA+TGG + Chr1:91713351-91713370 MsG0180005426.01.T01:exon 25.0%
CTTTGTATTAAGGATCTGTT+TGG + Chr1:91712692-91712711 MsG0180005426.01.T01:intron 30.0%
TAGGGAGATATATGAATAGA+AGG + Chr1:91712844-91712863 MsG0180005426.01.T01:intron 30.0%
TATGCTCGTACTTTGTATTA+AGG + Chr1:91712682-91712701 MsG0180005426.01.T01:intron 30.0%
TATTAAGGATCTGTTTGGAT+TGG + Chr1:91712697-91712716 MsG0180005426.01.T01:intron 30.0%
TATTTCATTCATTCCTATGG+TGG - Chr1:91712904-91712923 None:intergenic 30.0%
TTTATTTGTGACAGGAGAAA+TGG + Chr1:91712457-91712476 MsG0180005426.01.T01:intron 30.0%
! ATGAGGATGACATTTTTGTT+AGG + Chr1:91712825-91712844 MsG0180005426.01.T01:intron 30.0%
! TGAGGATGACATTTTTGTTA+GGG + Chr1:91712826-91712845 MsG0180005426.01.T01:intron 30.0%
! TTTTTGCATTCTGTTCATCT+TGG + Chr1:91712943-91712962 MsG0180005426.01.T01:intron 30.0%
AAACCAGTACTTGGTTGTTT+AGG - Chr1:91712485-91712504 None:intergenic 35.0%
AAGTGAAGAAGCTAACCTTA+GGG - Chr1:91712291-91712310 None:intergenic 35.0%
AGCAGGAGATAAGAAGAAAT+AGG + Chr1:91713330-91713349 MsG0180005426.01.T01:CDS 35.0%
ATACACTATCTATCCACCAT+AGG + Chr1:91712888-91712907 MsG0180005426.01.T01:intron 35.0%
ATTGAGATAATCGAAGAACG+AGG - Chr1:91712384-91712403 None:intergenic 35.0%
CAATAACAAGAACATGTTCC+GGG - Chr1:91713265-91713284 None:intergenic 35.0%
CCAATAACAAGAACATGTTC+CGG - Chr1:91713266-91713285 None:intergenic 35.0%
GAAATGAATACCTGTAGTGA+CGG - Chr1:91712357-91712376 None:intergenic 35.0%
TTGTTATGGCTTCACATGAT+GGG + Chr1:91713365-91713384 MsG0180005426.01.T01:three_prime_UTR 35.0%
TTGTTCGTTGGATTGAACAA+AGG + Chr1:91712503-91712522 MsG0180005426.01.T01:CDS 35.0%
! TTTGTTATGGCTTCACATGA+TGG + Chr1:91713364-91713383 MsG0180005426.01.T01:three_prime_UTR 35.0%
AAGGTTGCAAAGAGAAAGCT+TGG + Chr1:91713117-91713136 MsG0180005426.01.T01:CDS 40.0%
ATTCCTTCTTTGTGACGATG+TGG - Chr1:91712529-91712548 None:intergenic 40.0%
CAAGGTTGGAAAAGACAAGA+GGG + Chr1:91713089-91713108 MsG0180005426.01.T01:CDS 40.0%
CATCGTCACAAAGAAGGAAT+TGG + Chr1:91712529-91712548 MsG0180005426.01.T01:CDS 40.0%
CATTCTGTTCATCTTGGATG+TGG + Chr1:91712949-91712968 MsG0180005426.01.T01:intron 40.0%
CGTTCAGAAAACAAGCAAGA+GGG + Chr1:91712993-91713012 MsG0180005426.01.T01:intron 40.0%
CTTCTTCTTTGCACGTTTGT+GGG - Chr1:91713145-91713164 None:intergenic 40.0%
GAAGTGAAGAAGCTAACCTT+AGG - Chr1:91712292-91712311 None:intergenic 40.0%
TCAAGGTTGGAAAAGACAAG+AGG + Chr1:91713088-91713107 MsG0180005426.01.T01:CDS 40.0%
TCGTTCAGAAAACAAGCAAG+AGG + Chr1:91712992-91713011 MsG0180005426.01.T01:intron 40.0%
TCTTCTTCTTTGCACGTTTG+TGG - Chr1:91713146-91713165 None:intergenic 40.0%
! ATATTTTCAATGTGCAGGGC+TGG + Chr1:91713301-91713320 MsG0180005426.01.T01:intron 40.0%
! CCGGAACATGTTCTTGTTAT+TGG + Chr1:91713263-91713282 MsG0180005426.01.T01:intron 40.0%
!! AAAAGACAAGAGGGCACTTA+AGG + Chr1:91713098-91713117 MsG0180005426.01.T01:CDS 40.0%
AACCTCATTAGAGAGGTTGC+TGG + Chr1:91713030-91713049 MsG0180005426.01.T01:CDS 45.0%
ACCTCATTAGAGAGGTTGCT+GGG + Chr1:91713031-91713050 MsG0180005426.01.T01:CDS 45.0%
ACGTGCAAAGAAGAAGAGAG+AGG + Chr1:91713149-91713168 MsG0180005426.01.T01:CDS 45.0%
AGGAGATGTCTAATGTCCTC+AGG + Chr1:91713169-91713188 MsG0180005426.01.T01:CDS 45.0%
ATCACTGAGTTGCTCAAGGT+TGG + Chr1:91713075-91713094 MsG0180005426.01.T01:CDS 45.0%
CCAACGAACAAACCAGTACT+TGG - Chr1:91712494-91712513 None:intergenic 45.0%
CTAATGTCCTCAGGAAGATG+AGG + Chr1:91713178-91713197 MsG0180005426.01.T01:CDS 45.0%
GAACAAACCTCATCTTCCTG+AGG - Chr1:91713188-91713207 None:intergenic 45.0%
GCTCCTAAACAACCAAGTAC+TGG + Chr1:91712479-91712498 MsG0180005426.01.T01:CDS 45.0%
TCAGTGATACGCTTCTCGTA+AGG - Chr1:91713063-91713082 None:intergenic 45.0%
TGTGAGGAACCTCATTAGAG+AGG + Chr1:91713023-91713042 MsG0180005426.01.T01:CDS 45.0%
!! CCAAGTACTGGTTTGTTCGT+TGG + Chr1:91712491-91712510 MsG0180005426.01.T01:CDS 45.0%
ACCCAGCAACCTCTCTAATG+AGG - Chr1:91713035-91713054 None:intergenic 50.0%
AGGCCACATCGTCACAAAGA+AGG + Chr1:91712523-91712542 MsG0180005426.01.T01:CDS 50.0%
GCGTATCACTGAGTTGCTCA+AGG + Chr1:91713071-91713090 MsG0180005426.01.T01:CDS 50.0%
! TCTGAGATCACCGTCACTAC+AGG + Chr1:91712344-91712363 MsG0180005426.01.T01:CDS 50.0%
! TTTTCAATGTGCAGGGCTGG+TGG + Chr1:91713304-91713323 MsG0180005426.01.T01:intron 50.0%
ACCGCACTCTCTCTGGTGTT+AGG + Chr1:91712318-91712337 MsG0180005426.01.T01:CDS 55.0%
CCCTAACACCAGAGAGAGTG+CGG - Chr1:91712322-91712341 None:intergenic 55.0%
! CCGCACTCTCTCTGGTGTTA+GGG + Chr1:91712319-91712338 MsG0180005426.01.T01:CDS 55.0%
! GCAAGAGGGTGCACTTTGTG+AGG + Chr1:91713007-91713026 MsG0180005426.01.T01:CDS 55.0%
! TAACACCAGAGAGAGTGCGG+TGG - Chr1:91712319-91712338 None:intergenic 55.0%
ACGTCCTTCAGCTCGCAAGG+GGG + Chr1:91712556-91712575 MsG0180005426.01.T01:CDS 60.0%
CACGTCCTTCAGCTCGCAAG+GGG + Chr1:91712555-91712574 MsG0180005426.01.T01:CDS 60.0%
CCACGTCCTTCAGCTCGCAA+GGG + Chr1:91712554-91712573 MsG0180005426.01.T01:CDS 60.0%
CCATTCCACCGCACTCTCTC+TGG + Chr1:91712311-91712330 MsG0180005426.01.T01:CDS 60.0%
TCCACGTCCTTCAGCTCGCA+AGG + Chr1:91712553-91712572 MsG0180005426.01.T01:CDS 60.0%
! CCAGAGAGAGTGCGGTGGAA+TGG - Chr1:91712314-91712333 None:intergenic 60.0%
! TCAATGTGCAGGGCTGGTGG+TGG + Chr1:91713307-91713326 MsG0180005426.01.T01:intron 60.0%
CCCTTGCGAGCTGAAGGACG+TGG - Chr1:91712557-91712576 None:intergenic 65.0%
CTGACCCCCTTGCGAGCTGA+AGG - Chr1:91712563-91712582 None:intergenic 65.0%
!! TGCAGGGCTGGTGGTGGAGC+AGG + Chr1:91713313-91713332 MsG0180005426.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 91712287 91713580 91712287 ID=MsG0180005426.01;Name=MsG0180005426.01
Chr1 mRNA 91712287 91713580 91712287 ID=MsG0180005426.01.T01;Parent=MsG0180005426.01;Name=MsG0180005426.01.T01;_AED=0.28;_eAED=0.28;_QI=0|1|1|1|0.66|0.5|4|229|139
Chr1 exon 91712287 91712365 91712287 ID=MsG0180005426.01.T01:exon:48593;Parent=MsG0180005426.01.T01
Chr1 exon 91712471 91712577 91712471 ID=MsG0180005426.01.T01:exon:48594;Parent=MsG0180005426.01.T01
Chr1 exon 91713000 91713199 91713000 ID=MsG0180005426.01.T01:exon:48595;Parent=MsG0180005426.01.T01
Chr1 exon 91713318 91713580 91713318 ID=MsG0180005426.01.T01:exon:48596;Parent=MsG0180005426.01.T01
Chr1 CDS 91712287 91712365 91712287 ID=MsG0180005426.01.T01:cds;Parent=MsG0180005426.01.T01
Chr1 CDS 91712471 91712577 91712471 ID=MsG0180005426.01.T01:cds;Parent=MsG0180005426.01.T01
Chr1 CDS 91713000 91713199 91713000 ID=MsG0180005426.01.T01:cds;Parent=MsG0180005426.01.T01
Chr1 CDS 91713318 91713351 91713318 ID=MsG0180005426.01.T01:cds;Parent=MsG0180005426.01.T01
Chr1 three_prime_UTR 91713352 91713580 91713352 ID=MsG0180005426.01.T01:three_prime_utr;Parent=MsG0180005426.01.T01
Gene Sequence

>MsG0180005426.01.T01

ACCCTAAGGTTAGCTTCTTCACTTCCATTCCACCGCACTCTCTCTGGTGTTAGGGTTTCTGAGATCACCGTCACTACAGGAGAAATGGCTCCTAAACAACCAAGTACTGGTTTGTTCGTTGGATTGAACAAAGGCCACATCGTCACAAAGAAGGAATTGGCTCCACGTCCTTCAGCTCGCAAGGGGAAAACAAGCAAGAGGGTGCACTTTGTGAGGAACCTCATTAGAGAGGTTGCTGGGTTTGCTCCTTACGAGAAGCGTATCACTGAGTTGCTCAAGGTTGGAAAAGACAAGAGGGCACTTAAGGTTGCAAAGAGAAAGCTTGGAACCCACAAACGTGCAAAGAAGAAGAGAGAGGAGATGTCTAATGTCCTCAGGAAGATGAGGGCTGGTGGTGGAGCAGGAGATAAGAAGAAATAG

Protein sequence

>MsG0180005426.01.T01

TLRLASSLPFHRTLSGVRVSEITVTTGEMAPKQPSTGLFVGLNKGHIVTKKELAPRPSARKGKTSKRVHFVRNLIREVAGFAPYEKRITELLKVGKDKRALKVAKRKLGTHKRAKKKREEMSNVLRKMRAGGGAGDKKK*