Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010058.01.T01 | AES66638.1 | 95.405 | 457 | 20 | 1 | 1 | 456 | 1 | 457 | 0 | 905 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010058.01.T01 | Q147F9 | 52.518 | 417 | 196 | 2 | 25 | 439 | 30 | 446 | 2.53E-156 | 454 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010058.01.T01 | G7IH73 | 95.405 | 457 | 20 | 1 | 1 | 456 | 1 | 457 | 0.0 | 905 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000470.01 | MsG0280010058.01 | 0.805900 | 1.067707e-49 | 5.716248e-47 |
| MsG0180004765.01 | MsG0280010058.01 | 0.823028 | 1.726765e-53 | 1.469774e-50 |
| MsG0180005185.01 | MsG0280010058.01 | 0.802014 | 6.857945e-49 | 3.323254e-46 |
| MsG0280006601.01 | MsG0280010058.01 | 0.808152 | 3.564328e-50 | 2.022790e-47 |
| MsG0280007955.01 | MsG0280010058.01 | 0.820372 | 7.101683e-53 | 5.609157e-50 |
| MsG0280010058.01 | MsG0280010109.01 | 0.802449 | 5.582055e-49 | 2.735100e-46 |
| MsG0280010058.01 | MsG0380014103.01 | 0.813435 | 2.562861e-51 | 1.673597e-48 |
| MsG0280010058.01 | MsG0380014495.01 | 0.809101 | 2.234470e-50 | 1.300211e-47 |
| MsG0280010058.01 | MsG0380015332.01 | 0.805634 | 1.214531e-49 | 6.458000e-47 |
| MsG0280010058.01 | MsG0580027947.01 | 0.802053 | 6.732187e-49 | 3.265586e-46 |
| MsG0280010058.01 | MsG0680030447.01 | 0.803525 | 3.344021e-49 | 1.684311e-46 |
| MsG0280010058.01 | MsG0680031264.01 | 0.802749 | 4.839404e-49 | 2.389540e-46 |
| MsG0280010058.01 | MsG0680032112.01 | 0.814833 | 1.259407e-51 | 8.540921e-49 |
| MsG0280010058.01 | MsG0780037054.01 | 0.801898 | 7.247146e-49 | 3.501579e-46 |
| MsG0280010058.01 | MsG0780039170.01 | 0.814211 | 1.728901e-51 | 1.152936e-48 |
| MsG0280010058.01 | MsG0880042311.01 | 0.807949 | 3.936911e-50 | 2.222182e-47 |
| MsG0280010058.01 | MsG0880043480.01 | 0.803145 | 4.008898e-49 | 1.999399e-46 |
| MsG0280010058.01 | MsG0880044210.01 | 0.804503 | 2.093722e-49 | 1.081289e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010058.01.T01 | MTR_2g076690 | 95.405 | 457 | 20 | 1 | 1 | 456 | 1 | 457 | 0.0 | 905 |
| MsG0280010058.01.T01 | MTR_2g076840 | 83.778 | 450 | 69 | 4 | 7 | 455 | 1 | 447 | 0.0 | 780 |
| MsG0280010058.01.T01 | MTR_2g076840 | 65.632 | 419 | 92 | 7 | 44 | 460 | 361 | 729 | 4.73e-171 | 498 |
| MsG0280010058.01.T01 | MTR_2g076920 | 79.792 | 480 | 70 | 5 | 1 | 460 | 1 | 473 | 0.0 | 777 |
| MsG0280010058.01.T01 | MTR_2g076710 | 84.340 | 447 | 62 | 3 | 3 | 442 | 1 | 446 | 0.0 | 773 |
| MsG0280010058.01.T01 | MTR_2g076970 | 81.153 | 451 | 83 | 2 | 7 | 456 | 1 | 450 | 0.0 | 765 |
| MsG0280010058.01.T01 | MTR_2g076900 | 79.826 | 461 | 84 | 4 | 7 | 460 | 1 | 459 | 0.0 | 757 |
| MsG0280010058.01.T01 | MTR_2g076990 | 82.785 | 395 | 65 | 1 | 65 | 456 | 1 | 395 | 0.0 | 670 |
| MsG0280010058.01.T01 | MTR_8g076920 | 46.004 | 463 | 233 | 6 | 6 | 454 | 15 | 474 | 2.12e-148 | 432 |
| MsG0280010058.01.T01 | MTR_5g020980 | 45.648 | 471 | 242 | 7 | 1 | 461 | 7 | 473 | 3.39e-146 | 427 |
| MsG0280010058.01.T01 | MTR_5g020990 | 42.222 | 450 | 233 | 7 | 21 | 446 | 36 | 482 | 1.16e-118 | 356 |
| MsG0280010058.01.T01 | MTR_4g107370 | 41.649 | 485 | 227 | 10 | 16 | 454 | 15 | 489 | 1.96e-115 | 349 |
| MsG0280010058.01.T01 | MTR_1g022250 | 42.451 | 457 | 225 | 8 | 16 | 444 | 12 | 458 | 3.24e-112 | 339 |
| MsG0280010058.01.T01 | MTR_2g030420 | 40.570 | 456 | 244 | 6 | 16 | 450 | 15 | 464 | 2.02e-111 | 337 |
| MsG0280010058.01.T01 | MTR_7g008040 | 39.869 | 459 | 228 | 8 | 16 | 434 | 10 | 460 | 7.22e-107 | 328 |
| MsG0280010058.01.T01 | MTR_6g009720 | 38.961 | 462 | 231 | 9 | 16 | 433 | 14 | 468 | 2.84e-105 | 323 |
| MsG0280010058.01.T01 | MTR_6g009770 | 40.460 | 435 | 203 | 9 | 46 | 434 | 23 | 447 | 3.34e-103 | 317 |
| MsG0280010058.01.T01 | MTR_6g009553 | 39.825 | 457 | 211 | 11 | 30 | 434 | 4 | 448 | 3.42e-103 | 316 |
| MsG0280010058.01.T01 | MTR_6g009520 | 40.693 | 462 | 217 | 12 | 16 | 434 | 14 | 461 | 3.52e-103 | 317 |
| MsG0280010058.01.T01 | MTR_6g042290 | 39.207 | 454 | 238 | 11 | 5 | 434 | 12 | 451 | 5.45e-101 | 311 |
| MsG0280010058.01.T01 | MTR_4g128260 | 36.929 | 482 | 251 | 9 | 16 | 459 | 10 | 476 | 8.64e-101 | 311 |
| MsG0280010058.01.T01 | MTR_6g009540 | 39.059 | 489 | 244 | 12 | 16 | 458 | 12 | 492 | 7.63e-99 | 306 |
| MsG0280010058.01.T01 | MTR_1g022245 | 37.872 | 470 | 240 | 9 | 7 | 435 | 1 | 459 | 4.47e-98 | 304 |
| MsG0280010058.01.T01 | MTR_2g061820 | 35.876 | 485 | 248 | 12 | 14 | 456 | 3 | 466 | 5.28e-96 | 299 |
| MsG0280010058.01.T01 | MTR_3g103150 | 50.896 | 279 | 128 | 5 | 162 | 434 | 1 | 276 | 1.30e-90 | 277 |
| MsG0280010058.01.T01 | MTR_6g009510 | 36.795 | 443 | 219 | 9 | 5 | 434 | 12 | 406 | 2.23e-87 | 275 |
| MsG0280010058.01.T01 | MTR_6g009660 | 37.947 | 419 | 216 | 13 | 36 | 434 | 17 | 411 | 8.34e-78 | 250 |
| MsG0280010058.01.T01 | MTR_3g092060 | 34.037 | 379 | 212 | 10 | 68 | 436 | 66 | 416 | 1.39e-68 | 227 |
| MsG0280010058.01.T01 | MTR_5g021020 | 34.722 | 432 | 230 | 11 | 10 | 434 | 19 | 405 | 3.90e-67 | 221 |
| MsG0280010058.01.T01 | MTR_7g117950 | 33.146 | 356 | 200 | 8 | 65 | 414 | 61 | 384 | 2.42e-64 | 215 |
| MsG0280010058.01.T01 | MTR_8g027200 | 29.847 | 392 | 213 | 11 | 54 | 434 | 48 | 388 | 9.15e-48 | 171 |
| MsG0280010058.01.T01 | MTR_5g021000 | 62.857 | 105 | 37 | 1 | 332 | 434 | 1 | 105 | 1.63e-36 | 132 |
| MsG0280010058.01.T01 | MTR_2g076820 | 92.424 | 66 | 5 | 0 | 365 | 430 | 1 | 66 | 1.92e-36 | 129 |
| MsG0280010058.01.T01 | MTR_5g020720 | 42.778 | 180 | 75 | 4 | 264 | 434 | 25 | 185 | 2.39e-31 | 119 |
| MsG0280010058.01.T01 | MTR_1g011680 | 41.045 | 134 | 79 | 0 | 306 | 439 | 3 | 136 | 2.26e-29 | 113 |
| MsG0280010058.01.T01 | MTR_2g076910 | 68.000 | 75 | 23 | 1 | 7 | 81 | 1 | 74 | 5.46e-28 | 106 |
| MsG0280010058.01.T01 | MTR_5g021010 | 50.476 | 105 | 44 | 3 | 338 | 434 | 17 | 121 | 1.80e-21 | 90.1 |
| MsG0280010058.01.T01 | MTR_2g076830 | 76.596 | 47 | 11 | 0 | 403 | 449 | 9 | 55 | 7.43e-17 | 75.1 |
| MsG0280010058.01.T01 | MTR_3g062510 | 26.943 | 193 | 127 | 6 | 216 | 400 | 171 | 357 | 2.96e-14 | 74.7 |
| MsG0280010058.01.T01 | MTR_3g062510 | 28.485 | 165 | 106 | 4 | 216 | 372 | 171 | 331 | 4.33e-14 | 73.9 |
| MsG0280010058.01.T01 | MTR_3g117230 | 30.047 | 213 | 130 | 8 | 190 | 392 | 129 | 332 | 8.18e-14 | 73.6 |
| MsG0280010058.01.T01 | MTR_3g112260 | 26.855 | 283 | 184 | 10 | 190 | 458 | 126 | 399 | 1.54e-13 | 72.4 |
| MsG0280010058.01.T01 | MTR_4g094270 | 29.310 | 174 | 111 | 5 | 210 | 375 | 163 | 332 | 9.18e-13 | 70.1 |
| MsG0280010058.01.T01 | MTR_7g089040 | 31.579 | 152 | 89 | 5 | 228 | 371 | 408 | 552 | 1.91e-12 | 70.1 |
| MsG0280010058.01.T01 | MTR_7g010800 | 28.495 | 186 | 116 | 6 | 201 | 376 | 203 | 381 | 2.35e-12 | 69.7 |
| MsG0280010058.01.T01 | MTR_8g099605 | 30.178 | 169 | 102 | 4 | 221 | 375 | 179 | 345 | 9.62e-12 | 67.8 |
| MsG0280010058.01.T01 | MTR_8g099605 | 30.357 | 168 | 101 | 4 | 222 | 375 | 421 | 586 | 1.18e-11 | 67.8 |
| MsG0280010058.01.T01 | MTR_7g101870 | 24.194 | 248 | 144 | 9 | 161 | 372 | 136 | 375 | 3.09e-11 | 66.2 |
| MsG0280010058.01.T01 | MTR_7g101870 | 28.409 | 176 | 114 | 5 | 205 | 372 | 480 | 651 | 5.13e-11 | 65.5 |
| MsG0280010058.01.T01 | MTR_1g063380 | 29.341 | 167 | 106 | 5 | 213 | 371 | 487 | 649 | 4.05e-11 | 65.9 |
| MsG0280010058.01.T01 | MTR_1g113750 | 41.053 | 95 | 50 | 3 | 365 | 453 | 16 | 110 | 4.92e-11 | 60.5 |
| MsG0280010058.01.T01 | MTR_2g095210 | 29.341 | 167 | 106 | 5 | 213 | 371 | 415 | 577 | 5.74e-11 | 65.5 |
| MsG0280010058.01.T01 | MTR_3g023890 | 26.389 | 216 | 138 | 6 | 211 | 416 | 105 | 309 | 6.90e-11 | 65.1 |
| MsG0280010058.01.T01 | MTR_3g462930 | 30.460 | 174 | 109 | 5 | 204 | 369 | 304 | 473 | 6.95e-11 | 65.1 |
| MsG0280010058.01.T01 | MTR_3g104480 | 32.121 | 165 | 90 | 8 | 218 | 369 | 232 | 387 | 8.04e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010058.01.T01 | AT3G50940 | 52.518 | 417 | 196 | 2 | 25 | 439 | 30 | 446 | 2.57e-157 | 454 |
| MsG0280010058.01.T01 | AT3G50930 | 48.430 | 446 | 210 | 5 | 23 | 451 | 74 | 516 | 1.90e-149 | 438 |
| MsG0280010058.01.T01 | AT2G18193 | 43.556 | 450 | 217 | 7 | 16 | 446 | 20 | 451 | 6.48e-127 | 377 |
| MsG0280010058.01.T01 | AT5G17740 | 40.741 | 459 | 258 | 4 | 16 | 461 | 19 | 476 | 2.83e-122 | 367 |
| MsG0280010058.01.T01 | AT2G18190 | 42.366 | 465 | 238 | 6 | 4 | 449 | 5 | 458 | 1.04e-121 | 364 |
| MsG0280010058.01.T01 | AT5G17760 | 41.632 | 478 | 250 | 7 | 10 | 462 | 9 | 482 | 1.96e-121 | 364 |
| MsG0280010058.01.T01 | AT5G17730 | 41.060 | 453 | 245 | 10 | 10 | 446 | 9 | 455 | 4.29e-119 | 357 |
| MsG0280010058.01.T01 | AT3G28600 | 40.909 | 462 | 218 | 9 | 16 | 440 | 9 | 452 | 1.31e-112 | 340 |
| MsG0280010058.01.T01 | AT3G28510 | 40.263 | 457 | 230 | 8 | 16 | 437 | 13 | 461 | 1.74e-111 | 339 |
| MsG0280010058.01.T01 | AT3G28540 | 40.044 | 457 | 218 | 8 | 16 | 431 | 13 | 454 | 2.11e-109 | 333 |
| MsG0280010058.01.T01 | AT3G28540 | 40.044 | 457 | 218 | 8 | 16 | 431 | 13 | 454 | 2.69e-109 | 333 |
| MsG0280010058.01.T01 | AT3G28610 | 39.792 | 480 | 219 | 12 | 3 | 440 | 1 | 452 | 2.63e-108 | 329 |
| MsG0280010058.01.T01 | AT4G05380 | 41.015 | 473 | 236 | 11 | 3 | 446 | 1 | 459 | 2.98e-108 | 328 |
| MsG0280010058.01.T01 | AT3G28580 | 40.043 | 467 | 231 | 12 | 1 | 430 | 1 | 455 | 9.72e-108 | 328 |
| MsG0280010058.01.T01 | AT4G25835 | 39.359 | 437 | 222 | 8 | 16 | 419 | 10 | 436 | 4.08e-105 | 322 |
| MsG0280010058.01.T01 | AT5G40010 | 40.088 | 454 | 227 | 9 | 16 | 430 | 13 | 460 | 1.06e-103 | 319 |
| MsG0280010058.01.T01 | AT5G40000 | 38.958 | 480 | 239 | 10 | 7 | 450 | 4 | 465 | 1.04e-101 | 312 |
| MsG0280010058.01.T01 | AT5G17750 | 40.342 | 409 | 204 | 10 | 5 | 402 | 11 | 390 | 5.42e-101 | 308 |
| MsG0280010058.01.T01 | AT1G43910 | 37.792 | 471 | 245 | 8 | 4 | 442 | 1 | 455 | 2.94e-100 | 308 |
| MsG0280010058.01.T01 | AT5G57480 | 35.792 | 461 | 249 | 8 | 16 | 436 | 10 | 463 | 3.48e-100 | 310 |
| MsG0280010058.01.T01 | AT3G28570 | 38.622 | 479 | 242 | 13 | 2 | 455 | 3 | 454 | 1.20e-98 | 304 |
| MsG0280010058.01.T01 | AT3G28570 | 41.766 | 419 | 203 | 9 | 51 | 455 | 56 | 447 | 1.26e-98 | 303 |
| MsG0280010058.01.T01 | AT3G28600 | 38.901 | 455 | 198 | 8 | 16 | 440 | 9 | 413 | 2.63e-97 | 300 |
| MsG0280010058.01.T01 | AT4G30250 | 35.941 | 473 | 248 | 11 | 7 | 436 | 1 | 461 | 1.62e-96 | 300 |
| MsG0280010058.01.T01 | AT3G28520 | 39.278 | 471 | 243 | 8 | 18 | 454 | 1 | 462 | 1.65e-96 | 299 |
| MsG0280010058.01.T01 | AT4G30250 | 35.941 | 473 | 248 | 11 | 7 | 436 | 3 | 463 | 1.97e-96 | 300 |
| MsG0280010058.01.T01 | AT3G28580 | 37.885 | 454 | 237 | 11 | 1 | 430 | 1 | 433 | 5.86e-96 | 298 |
| MsG0280010058.01.T01 | AT2G46620 | 31.723 | 476 | 268 | 12 | 4 | 454 | 1 | 444 | 8.93e-75 | 243 |
| MsG0280010058.01.T01 | AT5G17760 | 39.329 | 328 | 180 | 5 | 10 | 322 | 9 | 332 | 4.60e-72 | 231 |
| MsG0280010058.01.T01 | AT3G29800 | 32.524 | 412 | 213 | 12 | 54 | 448 | 40 | 403 | 9.53e-64 | 213 |
| MsG0280010058.01.T01 | AT4G05340 | 60.294 | 68 | 27 | 0 | 322 | 389 | 26 | 93 | 3.17e-20 | 85.9 |
| MsG0280010058.01.T01 | AT3G28560 | 32.727 | 165 | 90 | 4 | 63 | 214 | 55 | 211 | 7.74e-18 | 83.6 |
| MsG0280010058.01.T01 | AT5G58290 | 26.341 | 205 | 137 | 6 | 204 | 400 | 152 | 350 | 9.96e-15 | 76.3 |
| MsG0280010058.01.T01 | AT1G45000 | 28.521 | 284 | 178 | 12 | 190 | 458 | 126 | 399 | 5.73e-14 | 73.9 |
| MsG0280010058.01.T01 | AT5G43010 | 30.256 | 195 | 118 | 7 | 190 | 375 | 126 | 311 | 6.44e-14 | 73.6 |
| MsG0280010058.01.T01 | AT5G19990 | 29.310 | 174 | 111 | 5 | 210 | 375 | 107 | 276 | 5.75e-13 | 70.5 |
| MsG0280010058.01.T01 | AT5G20000 | 29.310 | 174 | 111 | 5 | 210 | 375 | 165 | 334 | 9.69e-13 | 70.1 |
| MsG0280010058.01.T01 | AT5G19990 | 29.310 | 174 | 111 | 5 | 210 | 375 | 165 | 334 | 1.04e-12 | 70.1 |
| MsG0280010058.01.T01 | AT5G19990 | 29.310 | 174 | 111 | 5 | 210 | 375 | 165 | 334 | 1.04e-12 | 70.1 |
| MsG0280010058.01.T01 | AT2G03670 | 31.210 | 157 | 85 | 6 | 234 | 375 | 49 | 197 | 2.46e-11 | 66.2 |
| MsG0280010058.01.T01 | AT2G03670 | 31.210 | 157 | 85 | 6 | 234 | 375 | 49 | 197 | 2.98e-11 | 65.5 |
| MsG0280010058.01.T01 | AT3G53230 | 24.194 | 248 | 144 | 9 | 161 | 372 | 135 | 374 | 4.77e-11 | 65.9 |
| MsG0280010058.01.T01 | AT3G53230 | 29.167 | 168 | 107 | 5 | 213 | 372 | 487 | 650 | 6.19e-11 | 65.5 |
| MsG0280010058.01.T01 | AT3G09840 | 23.790 | 248 | 145 | 9 | 161 | 372 | 134 | 373 | 7.16e-11 | 65.1 |
| MsG0280010058.01.T01 | AT5G03340 | 23.790 | 248 | 145 | 9 | 161 | 372 | 134 | 373 | 7.29e-11 | 65.1 |
Find 72 sgRNAs with CRISPR-Local
Find 105 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAAGATTGAAGTTCTTCT+TGG | 0.223470 | 2:-63596852 | MsG0280010058.01.T01:CDS |
| CTTCTCTCTTAGTCTCTTCC+TGG | 0.235607 | 2:+63596721 | None:intergenic |
| TTTCAACAGATGACACTTTC+GGG | 0.253746 | 2:-63597080 | MsG0280010058.01.T01:intron |
| ATTGGATGGTTGAACTTAAT+AGG | 0.269533 | 2:+63597862 | None:intergenic |
| TTGAAATTCTTCAATGACTA+TGG | 0.286598 | 2:+63598284 | None:intergenic |
| AAGGGAAATTAAGAATGATT+TGG | 0.292518 | 2:-63597803 | MsG0280010058.01.T01:CDS |
| TTGTATGGTCCTCCTGGTAC+TGG | 0.303664 | 2:-63597715 | MsG0280010058.01.T01:CDS |
| GAAGATGAAGTTGTTCATAA+TGG | 0.306237 | 2:-63597517 | MsG0280010058.01.T01:CDS |
| AGTCCTAAACTTTGTTCAAT+TGG | 0.320894 | 2:-63598346 | MsG0280010058.01.T01:CDS |
| CAAGAATTCGACATTATGAT+AGG | 0.351643 | 2:-63598059 | MsG0280010058.01.T01:CDS |
| GTCCTAAACTTTGTTCAATT+GGG | 0.369166 | 2:-63598345 | MsG0280010058.01.T01:CDS |
| GTCCCAATTGAACAAAGTTT+AGG | 0.371014 | 2:+63598343 | None:intergenic |
| TGAATGCAGTAGATGGTCTT+TGG | 0.373799 | 2:-63597052 | MsG0280010058.01.T01:CDS |
| GAACAACTTCATCTTCTTCT+CGG | 0.380479 | 2:+63597524 | None:intergenic |
| TGTTCATAATGGGCACAATA+AGG | 0.386190 | 2:-63597506 | MsG0280010058.01.T01:intron |
| AGTTAAAGCAACCAAATCTA+AGG | 0.392234 | 2:-63598187 | MsG0280010058.01.T01:CDS |
| ACCGCGTTTCCAAGCTTTCC+CGG | 0.395418 | 2:+63597744 | None:intergenic |
| AGTTGCTTTAGTTCCTAGAT+AGG | 0.400041 | 2:+63598224 | None:intergenic |
| GGAATTTGATAAGGTCTTGC+AGG | 0.402644 | 2:+63596763 | None:intergenic |
| ACTTGGATCTCACTAATGTT+GGG | 0.408726 | 2:-63597639 | MsG0280010058.01.T01:CDS |
| AACTCTTCTGCAATTTCAGC+TGG | 0.411104 | 2:+63596813 | None:intergenic |
| AAGATGAAGTTGTTCATAAT+GGG | 0.412763 | 2:-63597516 | MsG0280010058.01.T01:CDS |
| GAGGCAGCAGAAGCCTATCT+AGG | 0.421960 | 2:-63598237 | MsG0280010058.01.T01:CDS |
| TGGCTTGGAGGAATTTGATA+AGG | 0.429033 | 2:+63596754 | None:intergenic |
| ATTCCTCCAAGCCAAGAAAA+TGG | 0.431539 | 2:-63596745 | MsG0280010058.01.T01:CDS |
| GAGGCCACTGCAGATAAAAC+TGG | 0.436495 | 2:+63598414 | None:intergenic |
| TGGTGAATTGTGCGTTGAAT+TGG | 0.444709 | 2:+63598304 | None:intergenic |
| CCGTTCTATACTTGTGATAG+AGG | 0.447088 | 2:-63597575 | MsG0280010058.01.T01:CDS |
| GACTTGGATCTCACTAATGT+TGG | 0.453484 | 2:-63597640 | MsG0280010058.01.T01:CDS |
| TTGAAGGTGTTAGTGTAAAG+TGG | 0.455356 | 2:-63598110 | MsG0280010058.01.T01:CDS |
| TACTTGTTGTATGGTCCTCC+TGG | 0.457478 | 2:-63597721 | MsG0280010058.01.T01:CDS |
| GCAAGAGTGTTGAAACTCAT+TGG | 0.462379 | 2:+63597844 | None:intergenic |
| TTAGCTCTCTCCATCACATA+AGG | 0.464927 | 2:+63597952 | None:intergenic |
| TCACAACAAATCACAAAGAC+AGG | 0.471357 | 2:-63596998 | MsG0280010058.01.T01:CDS |
| AAACGCGGTTACTTGTTGTA+TGG | 0.474020 | 2:-63597730 | MsG0280010058.01.T01:CDS |
| ATAGAAGAACCGGGAAAGCT+TGG | 0.478091 | 2:-63597753 | MsG0280010058.01.T01:CDS |
| AGCAAAGAAGAACAAGATCT+AGG | 0.484846 | 2:-63596693 | MsG0280010058.01.T01:CDS |
| CAATTGATGAAGAGCTTCAA+AGG | 0.486235 | 2:-63597822 | MsG0280010058.01.T01:CDS |
| ATAGTCATTGAAGAATTTCA+AGG | 0.496242 | 2:-63598282 | MsG0280010058.01.T01:CDS |
| GTCTAGCTTGATTGCAGCCA+TGG | 0.496694 | 2:-63597689 | MsG0280010058.01.T01:CDS |
| AAGCTAGACTTCCCAGTACC+AGG | 0.497146 | 2:+63597703 | None:intergenic |
| GGACTATTGAATGCAGTAGA+TGG | 0.497808 | 2:-63597059 | MsG0280010058.01.T01:CDS |
| CCAAGCCAAGAAAATGGTCC+AGG | 0.508568 | 2:-63596739 | MsG0280010058.01.T01:CDS |
| GATCTAGGCAGAGAAAATAT+AGG | 0.509105 | 2:-63596678 | MsG0280010058.01.T01:CDS |
| CAACTATGATATCTATGACT+TGG | 0.513130 | 2:-63597656 | MsG0280010058.01.T01:CDS |
| ATTTGCTTGTCCATTCTACC+AGG | 0.513687 | 2:+63596954 | None:intergenic |
| AGTTCGCATTAGTGCAATTG+AGG | 0.518879 | 2:+63598395 | None:intergenic |
| TTCATATTTGCCTTATGTGA+TGG | 0.523892 | 2:-63597962 | MsG0280010058.01.T01:CDS |
| TGTATGGTCCTCCTGGTACT+GGG | 0.528478 | 2:-63597714 | MsG0280010058.01.T01:CDS |
| AATTGATGAAGAGCTTCAAA+GGG | 0.533769 | 2:-63597821 | MsG0280010058.01.T01:CDS |
| TCTGTTAAGACCTGGTAGAA+TGG | 0.545715 | 2:-63596964 | MsG0280010058.01.T01:CDS |
| CTACCAGGTCTTAACAGAGC+AGG | 0.546075 | 2:+63596969 | None:intergenic |
| TATCACAAGTATAGAACGGT+TGG | 0.555140 | 2:+63597579 | None:intergenic |
| ACAAATTAAACAAGGAATTG+TGG | 0.559399 | 2:-63597929 | MsG0280010058.01.T01:CDS |
| AAGAATTCGACATTATGATA+GGG | 0.568700 | 2:-63598058 | MsG0280010058.01.T01:CDS |
| ACTCAAATGAATATAATCGT+TGG | 0.570247 | 2:-63597894 | MsG0280010058.01.T01:CDS |
| ACATTATGATAGGGACTCCT+CGG | 0.578511 | 2:-63598049 | MsG0280010058.01.T01:CDS |
| CTTGGATCTCACTAATGTTG+GGG | 0.595649 | 2:-63597638 | MsG0280010058.01.T01:CDS |
| GATCCTGCTCTGTTAAGACC+TGG | 0.601081 | 2:-63596972 | MsG0280010058.01.T01:CDS |
| GTTCATGTTCAGTAATGCAA+AGG | 0.603050 | 2:+63596880 | None:intergenic |
| TAGTCATTGAAGAATTTCAA+GGG | 0.606257 | 2:-63598281 | MsG0280010058.01.T01:CDS |
| GAGTGTTGAAACTCATTGGA+TGG | 0.615289 | 2:+63597848 | None:intergenic |
| ACCGGGAAAGCTTGGAAACG+CGG | 0.621197 | 2:-63597745 | MsG0280010058.01.T01:CDS |
| TCTTATTTCTGACACAGCCG+AGG | 0.630279 | 2:+63598032 | None:intergenic |
| GATGGTCTTTGGTCGTGCTG+TGG | 0.632541 | 2:-63597041 | MsG0280010058.01.T01:CDS |
| CCTCTATCACAAGTATAGAA+CGG | 0.641804 | 2:+63597575 | None:intergenic |
| TCTTTGGTCGTGCTGTGGAG+AGG | 0.654429 | 2:-63597036 | MsG0280010058.01.T01:CDS |
| ATAGTTGAGATAGTTAGCCA+TGG | 0.661661 | 2:+63597672 | None:intergenic |
| ACAAAGTTTAGGACTTCACG+AGG | 0.668909 | 2:+63598354 | None:intergenic |
| AGAGCTAAACAAATTAAACA+AGG | 0.691321 | 2:-63597937 | MsG0280010058.01.T01:CDS |
| AATTGCAGAAGAGTTAACAA+AGG | 0.702150 | 2:-63596805 | MsG0280010058.01.T01:CDS |
| CTAGACTTCCCAGTACCAGG+AGG | 0.750100 | 2:+63597706 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATTTAGCTGAAAAAATT+TGG | - | Chr2:63597809-63597828 | MsG0280010058.01.T01:CDS | 15.0% |
| !! | TCTTTCAAATAATTACAGTT+TGG | + | Chr2:63597683-63597702 | None:intergenic | 20.0% |
| !!! | AAAGAATTTTATAGAAGAAC+CGG | - | Chr2:63597352-63597371 | MsG0280010058.01.T01:intron | 20.0% |
| !!! | ATTAGTTTAAAATAGTTGAC+AGG | + | Chr2:63598001-63598020 | None:intergenic | 20.0% |
| ! | AAGAATTCGACATTATGATA+GGG | - | Chr2:63597057-63597076 | MsG0280010058.01.T01:CDS | 25.0% |
| ! | AAGATGAAGTTGTTCATAAT+GGG | - | Chr2:63597599-63597618 | MsG0280010058.01.T01:CDS | 25.0% |
| ! | AAGGGAAATTAAGAATGATT+TGG | - | Chr2:63597312-63597331 | MsG0280010058.01.T01:intron | 25.0% |
| ! | ACAAATTAAACAAGGAATTG+TGG | - | Chr2:63597186-63597205 | MsG0280010058.01.T01:intron | 25.0% |
| ! | ACTCAAATGAATATAATCGT+TGG | - | Chr2:63597221-63597240 | MsG0280010058.01.T01:intron | 25.0% |
| ! | AGAGCTAAACAAATTAAACA+AGG | - | Chr2:63597178-63597197 | MsG0280010058.01.T01:intron | 25.0% |
| ! | ATAAGTCATGTTGTTAACAA+AGG | + | Chr2:63597774-63597793 | None:intergenic | 25.0% |
| ! | ATAGTCATTGAAGAATTTCA+AGG | - | Chr2:63596833-63596852 | MsG0280010058.01.T01:CDS | 25.0% |
| ! | TAGTCATTGAAGAATTTCAA+GGG | - | Chr2:63596834-63596853 | MsG0280010058.01.T01:CDS | 25.0% |
| ! | TTGAAATTCTTCAATGACTA+TGG | + | Chr2:63596834-63596853 | None:intergenic | 25.0% |
| !! | AAGAATTTTATAGAAGAACC+GGG | - | Chr2:63597353-63597372 | MsG0280010058.01.T01:intron | 25.0% |
| !! | ATGACATGTATTTTCAAACT+AGG | - | Chr2:63597880-63597899 | MsG0280010058.01.T01:CDS | 25.0% |
| !! | CTTTTTATGTTCCTTAGATT+TGG | + | Chr2:63596942-63596961 | None:intergenic | 25.0% |
| !!! | CTTTTCTGCTTTTAAGAAAT+TGG | - | Chr2:63598202-63598221 | MsG0280010058.01.T01:CDS | 25.0% |
| AATTGATGAAGAGCTTCAAA+GGG | - | Chr2:63597294-63597313 | MsG0280010058.01.T01:intron | 30.0% | |
| AATTGCAGAAGAGTTAACAA+AGG | - | Chr2:63598310-63598329 | MsG0280010058.01.T01:CDS | 30.0% | |
| ACAGGAACAAAAGAATATGT+TGG | + | Chr2:63597983-63598002 | None:intergenic | 30.0% | |
| AGTCCTAAACTTTGTTCAAT+TGG | - | Chr2:63596769-63596788 | MsG0280010058.01.T01:CDS | 30.0% | |
| AGTTAAAGCAACCAAATCTA+AGG | - | Chr2:63596928-63596947 | MsG0280010058.01.T01:CDS | 30.0% | |
| ATGATGTCTGTTCATATCAT+GGG | - | Chr2:63597939-63597958 | MsG0280010058.01.T01:CDS | 30.0% | |
| ATTGGATGGTTGAACTTAAT+AGG | + | Chr2:63597256-63597275 | None:intergenic | 30.0% | |
| CAACTATGATATCTATGACT+TGG | - | Chr2:63597459-63597478 | MsG0280010058.01.T01:intron | 30.0% | |
| CAAGAATTCGACATTATGAT+AGG | - | Chr2:63597056-63597075 | MsG0280010058.01.T01:CDS | 30.0% | |
| GAAAAGATTGAAGTTCTTCT+TGG | - | Chr2:63598263-63598282 | MsG0280010058.01.T01:CDS | 30.0% | |
| GAAGATGAAGTTGTTCATAA+TGG | - | Chr2:63597598-63597617 | MsG0280010058.01.T01:CDS | 30.0% | |
| GAATTTGACATACATGAATC+TGG | - | Chr2:63597701-63597720 | MsG0280010058.01.T01:CDS | 30.0% | |
| GTCCTAAACTTTGTTCAATT+GGG | - | Chr2:63596770-63596789 | MsG0280010058.01.T01:CDS | 30.0% | |
| TATGATGTCTGTTCATATCA+TGG | - | Chr2:63597938-63597957 | MsG0280010058.01.T01:CDS | 30.0% | |
| TTCATATTTGCCTTATGTGA+TGG | - | Chr2:63597153-63597172 | MsG0280010058.01.T01:intron | 30.0% | |
| ! | TTCTCGGTTTTGCAATTTTA+CGG | + | Chr2:63597578-63597597 | None:intergenic | 30.0% |
| ! | TTTTCAACAGATGACACTTT+CGG | - | Chr2:63598034-63598053 | MsG0280010058.01.T01:CDS | 30.0% |
| !! | CTGAAAAAATTTGGTCTTGT+TGG | - | Chr2:63597818-63597837 | MsG0280010058.01.T01:CDS | 30.0% |
| AATCATCTGCATGATTTAGC+CGG | - | Chr2:63597737-63597756 | MsG0280010058.01.T01:CDS | 35.0% | |
| ACTTGGATCTCACTAATGTT+GGG | - | Chr2:63597476-63597495 | MsG0280010058.01.T01:intron | 35.0% | |
| AGCAAAGAAGAACAAGATCT+AGG | - | Chr2:63598422-63598441 | MsG0280010058.01.T01:CDS | 35.0% | |
| ATAGTTGAGATAGTTAGCCA+TGG | + | Chr2:63597446-63597465 | None:intergenic | 35.0% | |
| CAATTGATGAAGAGCTTCAA+AGG | - | Chr2:63597293-63597312 | MsG0280010058.01.T01:intron | 35.0% | |
| CCTCTATCACAAGTATAGAA+CGG | + | Chr2:63597543-63597562 | None:intergenic | 35.0% | |
| CGTTGAATTGGCAAAAAAGA+TGG | + | Chr2:63596802-63596821 | None:intergenic | 35.0% | |
| CTAAATATTATGTCCTCGTG+CGG | + | Chr2:63597798-63597817 | None:intergenic | 35.0% | |
| GAACAACTTCATCTTCTTCT+CGG | + | Chr2:63597594-63597613 | None:intergenic | 35.0% | |
| GATCTAGGCAGAGAAAATAT+AGG | - | Chr2:63598437-63598456 | MsG0280010058.01.T01:CDS | 35.0% | |
| GTCCCAATTGAACAAAGTTT+AGG | + | Chr2:63596775-63596794 | None:intergenic | 35.0% | |
| GTTCATGTTCAGTAATGCAA+AGG | + | Chr2:63598238-63598257 | None:intergenic | 35.0% | |
| TATCACAAGTATAGAACGGT+TGG | + | Chr2:63597539-63597558 | None:intergenic | 35.0% | |
| TCACAACAAATCACAAAGAC+AGG | - | Chr2:63598117-63598136 | MsG0280010058.01.T01:CDS | 35.0% | |
| TGATGTCTGTTCATATCATG+GGG | - | Chr2:63597940-63597959 | MsG0280010058.01.T01:CDS | 35.0% | |
| TTTCAACAGATGACACTTTC+GGG | - | Chr2:63598035-63598054 | MsG0280010058.01.T01:CDS | 35.0% | |
| ! | AAGATTTTGTCGACAATGGT+TGG | - | Chr2:63597842-63597861 | MsG0280010058.01.T01:CDS | 35.0% |
| ! | AGTTGCTTTAGTTCCTAGAT+AGG | + | Chr2:63596894-63596913 | None:intergenic | 35.0% |
| ! | GACAAGAAACCAAGTTTTTG+AGG | - | Chr2:63596859-63596878 | MsG0280010058.01.T01:CDS | 35.0% |
| ! | TGGTAAGATTTTGTCGACAA+TGG | - | Chr2:63597838-63597857 | MsG0280010058.01.T01:CDS | 35.0% |
| !! | GAAGTGAGTGATGTTTTTGA+AGG | - | Chr2:63596989-63597008 | MsG0280010058.01.T01:CDS | 35.0% |
| !! | TGTTCATAATGGGCACAATA+AGG | - | Chr2:63597609-63597628 | MsG0280010058.01.T01:CDS | 35.0% |
| !! | TTGAAGGTGTTAGTGTAAAG+TGG | - | Chr2:63597005-63597024 | MsG0280010058.01.T01:CDS | 35.0% |
| AACAAAGGACCAAAATTCGC+CGG | + | Chr2:63597759-63597778 | None:intergenic | 40.0% | |
| AACTCTTCTGCAATTTCAGC+TGG | + | Chr2:63598305-63598324 | None:intergenic | 40.0% | |
| ACATTATGATAGGGACTCCT+CGG | - | Chr2:63597066-63597085 | MsG0280010058.01.T01:CDS | 40.0% | |
| AGTTCGCATTAGTGCAATTG+AGG | + | Chr2:63596723-63596742 | None:intergenic | 40.0% | |
| ATTCCTCCAAGCCAAGAAAA+TGG | - | Chr2:63598370-63598389 | MsG0280010058.01.T01:CDS | 40.0% | |
| ATTTGCTTGTCCATTCTACC+AGG | + | Chr2:63598164-63598183 | None:intergenic | 40.0% | |
| CCGTTCTATACTTGTGATAG+AGG | - | Chr2:63597540-63597559 | MsG0280010058.01.T01:CDS | 40.0% | |
| CTTGGATCTCACTAATGTTG+GGG | - | Chr2:63597477-63597496 | MsG0280010058.01.T01:intron | 40.0% | |
| GACTTGGATCTCACTAATGT+TGG | - | Chr2:63597475-63597494 | MsG0280010058.01.T01:intron | 40.0% | |
| GGAATTTGATAAGGTCTTGC+AGG | + | Chr2:63598355-63598374 | None:intergenic | 40.0% | |
| GGACTATTGAATGCAGTAGA+TGG | - | Chr2:63598056-63598075 | MsG0280010058.01.T01:CDS | 40.0% | |
| TCTGTTAAGACCTGGTAGAA+TGG | - | Chr2:63598151-63598170 | MsG0280010058.01.T01:CDS | 40.0% | |
| TGAATGCAGTAGATGGTCTT+TGG | - | Chr2:63598063-63598082 | MsG0280010058.01.T01:CDS | 40.0% | |
| TGGTGAATTGTGCGTTGAAT+TGG | + | Chr2:63596814-63596833 | None:intergenic | 40.0% | |
| ! | ACAAAGTTTAGGACTTCACG+AGG | + | Chr2:63596764-63596783 | None:intergenic | 40.0% |
| ! | ATGATTTAGCCGGCGAATTT+TGG | - | Chr2:63597747-63597766 | MsG0280010058.01.T01:CDS | 40.0% |
| ! | TGGCTTGGAGGAATTTGATA+AGG | + | Chr2:63598364-63598383 | None:intergenic | 40.0% |
| ! | TTAGCTCTCTCCATCACATA+AGG | + | Chr2:63597166-63597185 | None:intergenic | 40.0% |
| !! | AAACGCGGTTACTTGTTGTA+TGG | - | Chr2:63597385-63597404 | MsG0280010058.01.T01:intron | 40.0% |
| !! | GAGTGTTGAAACTCATTGGA+TGG | + | Chr2:63597270-63597289 | None:intergenic | 40.0% |
| !! | GCAAGAGTGTTGAAACTCAT+TGG | + | Chr2:63597274-63597293 | None:intergenic | 40.0% |
| AACATGACTTATACCGCACG+AGG | - | Chr2:63597782-63597801 | MsG0280010058.01.T01:CDS | 45.0% | |
| ATAGAAGAACCGGGAAAGCT+TGG | - | Chr2:63597362-63597381 | MsG0280010058.01.T01:intron | 45.0% | |
| CTTCTCTCTTAGTCTCTTCC+TGG | + | Chr2:63598397-63598416 | None:intergenic | 45.0% | |
| CTTCTGCTGCCTCAAAAACT+TGG | + | Chr2:63596871-63596890 | None:intergenic | 45.0% | |
| ! | CTCTTCCTGGACCATTTTCT+TGG | + | Chr2:63598384-63598403 | None:intergenic | 45.0% |
| ! | TCGACAATGGTTGGTCAAGT+TGG | - | Chr2:63597851-63597870 | MsG0280010058.01.T01:CDS | 45.0% |
| !! | CAAGCCAGTTTTATCTGCAG+TGG | - | Chr2:63596697-63596716 | MsG0280010058.01.T01:CDS | 45.0% |
| !! | TACTTGTTGTATGGTCCTCC+TGG | - | Chr2:63597394-63597413 | MsG0280010058.01.T01:intron | 45.0% |
| !! | TCTTATTTCTGACACAGCCG+AGG | + | Chr2:63597086-63597105 | None:intergenic | 45.0% |
| AAGCTAGACTTCCCAGTACC+AGG | + | Chr2:63597415-63597434 | None:intergenic | 50.0% | |
| CCAAGCCAAGAAAATGGTCC+AGG | - | Chr2:63598376-63598395 | MsG0280010058.01.T01:CDS | 50.0% | |
| CTACCAGGTCTTAACAGAGC+AGG | + | Chr2:63598149-63598168 | None:intergenic | 50.0% | |
| GAGGCCACTGCAGATAAAAC+TGG | + | Chr2:63596704-63596723 | None:intergenic | 50.0% | |
| GATCCTGCTCTGTTAAGACC+TGG | - | Chr2:63598143-63598162 | MsG0280010058.01.T01:CDS | 50.0% | |
| TGTATGGTCCTCCTGGTACT+GGG | - | Chr2:63597401-63597420 | MsG0280010058.01.T01:intron | 50.0% | |
| TTGTATGGTCCTCCTGGTAC+TGG | - | Chr2:63597400-63597419 | MsG0280010058.01.T01:intron | 50.0% | |
| ! | CCTGGACCATTTTCTTGGCT+TGG | + | Chr2:63598379-63598398 | None:intergenic | 50.0% |
| ! | GTCTAGCTTGATTGCAGCCA+TGG | - | Chr2:63597426-63597445 | MsG0280010058.01.T01:intron | 50.0% |
| !!! | GGACCATTTTCTTGGCTTGG+AGG | + | Chr2:63598376-63598395 | None:intergenic | 50.0% |
| ACCGCGTTTCCAAGCTTTCC+CGG | + | Chr2:63597374-63597393 | None:intergenic | 55.0% | |
| ACCGGGAAAGCTTGGAAACG+CGG | - | Chr2:63597370-63597389 | MsG0280010058.01.T01:intron | 55.0% | |
| CTAGACTTCCCAGTACCAGG+AGG | + | Chr2:63597412-63597431 | None:intergenic | 55.0% | |
| GAGGCAGCAGAAGCCTATCT+AGG | - | Chr2:63596878-63596897 | MsG0280010058.01.T01:CDS | 55.0% | |
| GATGGTCTTTGGTCGTGCTG+TGG | - | Chr2:63598074-63598093 | MsG0280010058.01.T01:CDS | 55.0% | |
| TCTTTGGTCGTGCTGTGGAG+AGG | - | Chr2:63598079-63598098 | MsG0280010058.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 63596665 | 63598472 | 63596665 | ID=MsG0280010058.01;Name=MsG0280010058.01 |
| Chr2 | mRNA | 63596665 | 63598472 | 63596665 | ID=MsG0280010058.01.T01;Parent=MsG0280010058.01;Name=MsG0280010058.01.T01;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|1|1|2|0|464 |
| Chr2 | exon | 63597507 | 63598472 | 63597507 | ID=MsG0280010058.01.T01:exon:25312;Parent=MsG0280010058.01.T01 |
| Chr2 | exon | 63596665 | 63597093 | 63596665 | ID=MsG0280010058.01.T01:exon:25311;Parent=MsG0280010058.01.T01 |
| Chr2 | CDS | 63597507 | 63598472 | 63597507 | ID=MsG0280010058.01.T01:cds;Parent=MsG0280010058.01.T01 |
| Chr2 | CDS | 63596665 | 63597093 | 63596665 | ID=MsG0280010058.01.T01:cds;Parent=MsG0280010058.01.T01 |
| Gene Sequence |
| Protein sequence |
