AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380014867.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014867.01.T01 MTR_3g465410 93.007 143 10 0 1 143 1 143 4.67e-95 271
MsG0380014867.01.T01 MTR_4g063790 91.608 143 12 0 1 143 29 171 1.30e-92 265
MsG0380014867.01.T01 MTR_3g467080 78.195 133 29 0 1 133 29 161 2.48e-70 209
MsG0380014867.01.T01 MTR_3g466980 49.306 144 70 2 1 142 29 171 2.61e-42 140
MsG0380014867.01.T01 MTR_3g031240 47.407 135 71 0 1 135 29 163 2.83e-38 128
MsG0380014867.01.T01 MTR_3g065100 47.244 127 66 1 4 130 32 157 2.32e-36 125
MsG0380014867.01.T01 MTR_2g016210 43.885 139 78 0 1 139 29 167 7.38e-36 124
MsG0380014867.01.T01 MTR_3g031100 42.446 139 80 0 1 139 29 167 3.46e-33 117
MsG0380014867.01.T01 MTR_5g075380 40.909 132 71 2 1 129 29 156 4.02e-32 112
MsG0380014867.01.T01 MTR_4g032620 39.416 137 83 0 1 137 29 165 1.03e-31 113
MsG0380014867.01.T01 MTR_4g032290 40.299 134 80 0 4 137 32 165 4.43e-31 111
MsG0380014867.01.T01 MTR_2g035580 47.200 125 65 1 2 126 30 153 1.00e-29 105
MsG0380014867.01.T01 MTR_4g031910 38.806 134 82 0 4 137 32 165 6.57e-29 105
MsG0380014867.01.T01 MTR_4g032260 35.938 128 82 0 4 131 32 159 9.00e-28 100
MsG0380014867.01.T01 MTR_8g036130 39.189 148 79 2 1 137 29 176 4.87e-27 101
MsG0380014867.01.T01 MTR_2g035610 46.512 86 46 0 4 89 32 117 2.95e-25 93.6
MsG0380014867.01.T01 MTR_5g047580 38.095 126 76 2 1 126 29 152 6.57e-23 88.6
MsG0380014867.01.T01 MTR_1g077320 36.719 128 79 2 4 131 32 157 2.08e-22 87.4
MsG0380014867.01.T01 MTR_1g077300 35.385 130 80 2 4 133 32 157 2.35e-22 87.0
MsG0380014867.01.T01 MTR_4g028720 35.938 128 80 2 4 131 32 157 2.78e-22 87.0
MsG0380014867.01.T01 MTR_3g466830 36.434 129 81 1 1 129 28 155 6.13e-22 86.3
MsG0380014867.01.T01 MTR_1g077390 36.641 131 81 2 1 131 29 157 1.03e-21 85.5
MsG0380014867.01.T01 MTR_7g011950 35.156 128 81 2 4 131 32 157 1.16e-21 85.5
MsG0380014867.01.T01 MTR_1g084950 34.127 126 81 2 4 129 32 155 1.89e-21 84.7
MsG0380014867.01.T01 MTR_3g466930 34.921 126 81 1 4 129 2 126 4.33e-20 80.5
MsG0380014867.01.T01 MTR_1g090783 34.921 126 80 2 1 126 28 151 1.20e-19 80.1
MsG0380014867.01.T01 MTR_3g466900 33.333 126 83 1 4 129 2 126 3.15e-18 75.9
MsG0380014867.01.T01 MTR_3g466890 33.594 128 84 1 2 129 29 155 2.76e-17 73.9
MsG0380014867.01.T01 MTR_1g090710 31.746 126 85 1 1 126 29 153 4.18e-16 70.9
MsG0380014867.01.T01 MTR_7g055800 37.931 87 39 1 3 89 18 89 1.58e-15 67.8
MsG0380014867.01.T01 MTR_1g090697 32.812 128 80 4 1 126 29 152 7.47e-15 67.8
MsG0380014867.01.T01 MTR_5g047560 35.398 113 71 2 14 126 26 136 2.51e-14 65.9
MsG0380014867.01.T01 MTR_7g055940 38.158 76 32 1 3 78 18 78 9.92e-14 62.8
MsG0380014867.01.T01 MTR_4g019670 32.727 110 64 2 1 107 32 134 2.95e-12 63.2
MsG0380014867.01.T01 MTR_7g106510 31.579 114 63 3 1 111 32 133 4.94e-12 62.4
MsG0380014867.01.T01 MTR_4g028800 50.000 44 22 0 1 44 27 70 1.45e-11 57.0
MsG0380014867.01.T01 MTR_4g084780 53.704 54 25 0 46 99 1 54 7.64e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014867.01.T01 AT5G48670 46.825 126 62 3 4 127 32 154 4.81e-31 113
MsG0380014867.01.T01 AT5G27810 52.809 89 42 0 1 89 1 89 2.30e-29 103
MsG0380014867.01.T01 AT1G65300 39.552 134 79 2 2 135 29 160 5.19e-23 91.7
MsG0380014867.01.T01 AT1G65330 39.683 126 75 1 1 126 28 152 1.38e-22 90.5
MsG0380014867.01.T01 AT3G05860 36.806 144 86 2 1 142 29 169 4.23e-21 85.1
MsG0380014867.01.T01 AT3G05860 36.806 144 86 2 1 142 29 169 5.18e-21 85.9
MsG0380014867.01.T01 AT3G05860 36.806 144 86 2 1 142 29 169 7.91e-21 85.5
MsG0380014867.01.T01 AT5G26650 34.586 133 78 2 1 129 27 154 6.11e-20 84.7
MsG0380014867.01.T01 AT5G26630 35.537 121 77 1 6 126 34 153 6.55e-20 82.4
MsG0380014867.01.T01 AT1G31640 37.037 135 71 4 2 130 29 155 1.83e-19 83.6
MsG0380014867.01.T01 AT5G27960 43.529 85 48 0 1 85 27 111 3.72e-18 79.3
MsG0380014867.01.T01 AT1G31630 33.333 129 82 3 3 130 30 155 6.69e-17 75.9
MsG0380014867.01.T01 AT1G22590 37.647 85 53 0 1 85 29 113 1.23e-15 69.7
MsG0380014867.01.T01 AT2G28700 31.081 148 91 4 1 143 29 170 7.87e-13 64.7
MsG0380014867.01.T01 AT5G06500 27.907 129 85 3 1 126 29 152 7.44e-12 61.2

Find 34 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGGTCATTTCCATCTTTC+TGG 0.077675 3:+61690095 None:intergenic
ATTCAAACCATAGAATTTAA+TGG 0.173791 3:-61690057 MsG0380014867.01.T01:CDS
CGAATTTCCATTAAATTCTA+TGG 0.218755 3:+61690050 None:intergenic
GAGGTTTGGCCATCAGATTC+AGG 0.258145 3:-61690252 MsG0380014867.01.T01:CDS
CAAGAGGAGGTTGGAAATGA+GGG 0.355119 3:-61689934 MsG0380014867.01.T01:CDS
TCTTCTTGAGGCAAAACATT+AGG 0.355934 3:-61690155 MsG0380014867.01.T01:CDS
AATATAATGATCAATGATAT+TGG 0.368342 3:+61690077 None:intergenic
GAAGTGTGATATCTAGGTTT+AGG 0.426765 3:-61690224 MsG0380014867.01.T01:CDS
TCAAGAGGAGGTTGGAAATG+AGG 0.431693 3:-61689935 MsG0380014867.01.T01:CDS
GGGTCAGAAGTGTGATATCT+AGG 0.436600 3:-61690230 MsG0380014867.01.T01:CDS
TCTAGGTTTAGGAGTTTGCC+TGG 0.445921 3:-61690213 MsG0380014867.01.T01:CDS
AGAATTTGAAGGAGGTTGAT+CGG 0.478161 3:-61689984 MsG0380014867.01.T01:CDS
ACAACCCTCGAGTAGAGGTT+TGG 0.487846 3:-61690266 MsG0380014867.01.T01:CDS
AGGTTTGGCCATCAGATTCA+GGG 0.511068 3:-61690251 MsG0380014867.01.T01:CDS
ACGAGCAGTTGAAGAAGCAA+AGG 0.512962 3:-61690125 MsG0380014867.01.T01:CDS
AAGGGAGGAAACCAGAAAGA+TGG 0.513695 3:-61690106 MsG0380014867.01.T01:CDS
ATCAAAGATCAAGAGGAGGT+TGG 0.534167 3:-61689943 MsG0380014867.01.T01:CDS
AATGACAATCAAAGATCAAG+AGG 0.572865 3:-61689950 MsG0380014867.01.T01:CDS
CGAGCAGTTGAAGAAGCAAA+GGG 0.580872 3:-61690124 MsG0380014867.01.T01:CDS
ACATGCTTCGACTCCACATA+GGG 0.581479 3:+61690308 None:intergenic
AAGAGGAGGTTGGAAATGAG+GGG 0.584160 3:-61689933 MsG0380014867.01.T01:CDS
TATTGATGAGAATTTGAAGG+AGG 0.592173 3:-61689992 MsG0380014867.01.T01:CDS
CACATGCTTCGACTCCACAT+AGG 0.603214 3:+61690307 None:intergenic
AATGAAATTAGCACCCTATG+TGG 0.604835 3:-61690321 MsG0380014867.01.T01:CDS
AGAGGAGGTTGGAAATGAGG+GGG 0.608627 3:-61689932 MsG0380014867.01.T01:CDS
GCAAAACAACCCTCGAGTAG+AGG 0.609086 3:-61690271 MsG0380014867.01.T01:CDS
CTTCTGACCCCTGAATCTGA+TGG 0.624024 3:+61690243 None:intergenic
GATGGCCAAACCTCTACTCG+AGG 0.634480 3:+61690261 None:intergenic
GAGGAGGTTGGAAATGAGGG+GGG 0.643505 3:-61689931 MsG0380014867.01.T01:CDS
GACAATCAAAGATCAAGAGG+AGG 0.656411 3:-61689947 MsG0380014867.01.T01:CDS
GGTTTGGCCATCAGATTCAG+GGG 0.657992 3:-61690250 MsG0380014867.01.T01:CDS
ATGGCCAAACCTCTACTCGA+GGG 0.659257 3:+61690262 None:intergenic
GCAGTTGAAGAAGCAAAGGG+AGG 0.693727 3:-61690121 MsG0380014867.01.T01:CDS
TGGATCAGGAGGTCTTCTTG+AGG 0.700337 3:-61690167 MsG0380014867.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATAATGATCAATGATAT+TGG + Chr3:61690183-61690202 None:intergenic 15.0%
!! ATTCAAACCATAGAATTTAA+TGG - Chr3:61690200-61690219 MsG0380014867.01.T01:CDS 20.0%
!!! TTTTATTGATGAGAATTTGA+AGG - Chr3:61690262-61690281 MsG0380014867.01.T01:CDS 20.0%
! ACATGAACAAAGTAAAAAGA+TGG - Chr3:61690067-61690086 MsG0380014867.01.T01:CDS 25.0%
! CGAATTTCCATTAAATTCTA+TGG + Chr3:61690210-61690229 None:intergenic 25.0%
AATGACAATCAAAGATCAAG+AGG - Chr3:61690307-61690326 MsG0380014867.01.T01:CDS 30.0%
TATTGATGAGAATTTGAAGG+AGG - Chr3:61690265-61690284 MsG0380014867.01.T01:CDS 30.0%
TGAACAAAGTAAAAAGATGG+TGG - Chr3:61690070-61690089 MsG0380014867.01.T01:CDS 30.0%
! TTTTTACTTTGTTCATGTCC+AGG + Chr3:61690065-61690084 None:intergenic 30.0%
AATGAAATTAGCACCCTATG+TGG - Chr3:61689936-61689955 MsG0380014867.01.T01:CDS 35.0%
ATTGGTCATTTCCATCTTTC+TGG + Chr3:61690165-61690184 None:intergenic 35.0%
GAAGTGTGATATCTAGGTTT+AGG - Chr3:61690033-61690052 MsG0380014867.01.T01:CDS 35.0%
TCTTCTTGAGGCAAAACATT+AGG - Chr3:61690102-61690121 MsG0380014867.01.T01:CDS 35.0%
! AGAATTTGAAGGAGGTTGAT+CGG - Chr3:61690273-61690292 MsG0380014867.01.T01:CDS 35.0%
!! AAGTAAAAAGATGGTGGATC+AGG - Chr3:61690076-61690095 MsG0380014867.01.T01:CDS 35.0%
ATCAAAGATCAAGAGGAGGT+TGG - Chr3:61690314-61690333 MsG0380014867.01.T01:CDS 40.0%
GACAATCAAAGATCAAGAGG+AGG - Chr3:61690310-61690329 MsG0380014867.01.T01:CDS 40.0%
!! TAAAAAGATGGTGGATCAGG+AGG - Chr3:61690079-61690098 MsG0380014867.01.T01:CDS 40.0%
AAGAGGAGGTTGGAAATGAG+GGG - Chr3:61690324-61690343 MsG0380014867.01.T01:CDS 45.0%
AAGGGAGGAAACCAGAAAGA+TGG - Chr3:61690151-61690170 MsG0380014867.01.T01:CDS 45.0%
ACATGCTTCGACTCCACATA+GGG + Chr3:61689952-61689971 None:intergenic 45.0%
ACGAGCAGTTGAAGAAGCAA+AGG - Chr3:61690132-61690151 MsG0380014867.01.T01:CDS 45.0%
AGGTTTGGCCATCAGATTCA+GGG - Chr3:61690006-61690025 MsG0380014867.01.T01:CDS 45.0%
CAAGAGGAGGTTGGAAATGA+GGG - Chr3:61690323-61690342 MsG0380014867.01.T01:CDS 45.0%
CGAGCAGTTGAAGAAGCAAA+GGG - Chr3:61690133-61690152 MsG0380014867.01.T01:CDS 45.0%
TCAAGAGGAGGTTGGAAATG+AGG - Chr3:61690322-61690341 MsG0380014867.01.T01:CDS 45.0%
TCTAGGTTTAGGAGTTTGCC+TGG - Chr3:61690044-61690063 MsG0380014867.01.T01:CDS 45.0%
! GGGTCAGAAGTGTGATATCT+AGG - Chr3:61690027-61690046 MsG0380014867.01.T01:CDS 45.0%
ACAACCCTCGAGTAGAGGTT+TGG - Chr3:61689991-61690010 MsG0380014867.01.T01:CDS 50.0%
AGAGGAGGTTGGAAATGAGG+GGG - Chr3:61690325-61690344 MsG0380014867.01.T01:CDS 50.0%
ATGGCCAAACCTCTACTCGA+GGG + Chr3:61689998-61690017 None:intergenic 50.0%
CACATGCTTCGACTCCACAT+AGG + Chr3:61689953-61689972 None:intergenic 50.0%
CTTCTGACCCCTGAATCTGA+TGG + Chr3:61690017-61690036 None:intergenic 50.0%
GAGGTTTGGCCATCAGATTC+AGG - Chr3:61690005-61690024 MsG0380014867.01.T01:CDS 50.0%
GCAAAACAACCCTCGAGTAG+AGG - Chr3:61689986-61690005 MsG0380014867.01.T01:CDS 50.0%
GCAGTTGAAGAAGCAAAGGG+AGG - Chr3:61690136-61690155 MsG0380014867.01.T01:CDS 50.0%
GGTTTGGCCATCAGATTCAG+GGG - Chr3:61690007-61690026 MsG0380014867.01.T01:CDS 50.0%
TGGATCAGGAGGTCTTCTTG+AGG - Chr3:61690090-61690109 MsG0380014867.01.T01:CDS 50.0%
GAGGAGGTTGGAAATGAGGG+GGG - Chr3:61690326-61690345 MsG0380014867.01.T01:CDS 55.0%
GATGGCCAAACCTCTACTCG+AGG + Chr3:61689999-61690018 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 61689924 61690355 61689924 ID=MsG0380014867.01;Name=MsG0380014867.01
Chr3 mRNA 61689924 61690355 61689924 ID=MsG0380014867.01.T01;Parent=MsG0380014867.01;Name=MsG0380014867.01.T01;_AED=0.23;_eAED=0.23;_QI=0|-1|0|1|-1|1|1|0|143
Chr3 exon 61689924 61690355 61689924 ID=MsG0380014867.01.T01:exon:29326;Parent=MsG0380014867.01.T01
Chr3 CDS 61689924 61690355 61689924 ID=MsG0380014867.01.T01:cds;Parent=MsG0380014867.01.T01
Gene Sequence

>MsG0380014867.01.T01

ATGAAGAAGATTAATGAAATTAGCACCCTATGTGGAGTCGAAGCATGTGCAATAATTTCAGAGCAAAACAACCCTCGAGTAGAGGTTTGGCCATCAGATTCAGGGGTCAGAAGTGTGATATCTAGGTTTAGGAGTTTGCCTGGACATGAACAAAGTAAAAAGATGGTGGATCAGGAGGTCTTCTTGAGGCAAAACATTAGGAAAGTCTACGAGCAGTTGAAGAAGCAAAGGGAGGAAACCAGAAAGATGGAAATGACCAATATCATTGATCATTATATTCAAACCATAGAATTTAATGGAAATTCGATGAGCAAATCTGATCTTAATGATTTTTCTTCTTTTATTGATGAGAATTTGAAGGAGGTTGATCGGAAGATGAAAGAAATGACAATCAAAGATCAAGAGGAGGTTGGAAATGAGGGGGGATTATGA

Protein sequence

>MsG0380014867.01.T01

MKKINEISTLCGVEACAIISEQNNPRVEVWPSDSGVRSVISRFRSLPGHEQSKKMVDQEVFLRQNIRKVYEQLKKQREETRKMEMTNIIDHYIQTIEFNGNSMSKSDLNDFSSFIDENLKEVDRKMKEMTIKDQEEVGNEGGL*