AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035896.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035896.01.T01 MTR_7g039210 27.928 222 130 4 26 217 9 230 1.89e-28 108
MsG0680035896.01.T01 MTR_1g041870 28.571 196 112 3 45 240 7 174 1.09e-26 102
MsG0680035896.01.T01 MTR_1g054495 25.676 222 164 1 18 239 1 221 1.35e-26 103
MsG0680035896.01.T01 MTR_1g054485 29.412 187 132 0 18 204 1 187 1.53e-26 102
MsG0680035896.01.T01 MTR_4g085790 29.646 226 150 3 18 239 1 221 1.19e-25 100
MsG0680035896.01.T01 MTR_7g025150 30.702 228 145 5 18 239 1 221 4.43e-24 96.3
MsG0680035896.01.T01 MTR_0014s0220 27.442 215 139 2 25 239 626 823 1.35e-21 93.6
MsG0680035896.01.T01 MTR_4g123470 27.053 207 151 0 32 238 18 224 3.29e-21 89.0
MsG0680035896.01.T01 MTR_4g053550 36.111 72 46 0 41 112 47 118 1.35e-11 60.5
MsG0680035896.01.T01 MTR_4g069750 35.052 97 63 0 18 114 1 97 2.66e-11 58.9
MsG0680035896.01.T01 MTR_5g074790 29.358 109 77 0 31 139 161 269 3.00e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 57 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACAACCCTCTATCTTTC+TGG 0.158834 6:+114102000 MsG0680035896.01.T01:CDS
CCGATTAATCAGCAGGATTT+TGG 0.187815 6:+114102091 MsG0680035896.01.T01:intron
TTTCACCTATGGAGAATTAT+TGG 0.194379 6:+114101947 MsG0680035896.01.T01:CDS
AAAACATGAACCTTCTTCTT+AGG 0.199782 6:-114102761 None:intergenic
GAGGTTGTTCTCTTCTACTA+TGG 0.301858 6:+114102289 MsG0680035896.01.T01:CDS
TACTGTTGCTGCCTCTCATT+TGG 0.302835 6:+114102435 MsG0680035896.01.T01:CDS
TACTATGGTAACGATCTCTT+TGG 0.314615 6:+114102304 MsG0680035896.01.T01:CDS
AAGGAGTTCTGTAAGGAAAT+TGG 0.327796 6:+114102692 MsG0680035896.01.T01:CDS
CACTCCTATTGCCGTCTTCA+TGG 0.357892 6:-114102663 None:intergenic
CAACTCTCATTCCACGACTA+TGG 0.397696 6:-114102375 None:intergenic
AGAGAACAACCTCCTGGTTT+CGG 0.410476 6:-114102279 None:intergenic
TTCCTTGATGAACAACCATA+TGG 0.430156 6:+114101973 MsG0680035896.01.T01:CDS
CAACAGTAAAAGCATTCAAC+TGG 0.433820 6:-114102420 None:intergenic
TAGTAAGAAGCTCGAAAATC+CGG 0.435576 6:+114102336 MsG0680035896.01.T01:CDS
TCAGTAAGAAGTGGATGAAC+AGG 0.440343 6:-114102220 None:intergenic
CCCTCCATGAAGACGGCAAT+AGG 0.440838 6:+114102659 MsG0680035896.01.T01:CDS
ACTATGGTAACGATCTCTTT+GGG 0.443044 6:+114102305 MsG0680035896.01.T01:CDS
TTACCTTGCCAGAAAGATAG+AGG 0.455528 6:-114102008 None:intergenic
GTTCATCCACTTCTTACTGA+TGG 0.459428 6:+114102223 MsG0680035896.01.T01:CDS
GAGAATCCATAGGGAGTTTG+AGG 0.460901 6:+114102547 MsG0680035896.01.T01:intron
CTCCATATGGTTGTTCATCA+AGG 0.462097 6:-114101975 None:intergenic
CATAGTCCTCAAACTCCCTA+TGG 0.468898 6:-114102553 None:intergenic
GAGGACTATGTTAGAGCTTG+TGG 0.470662 6:+114102566 MsG0680035896.01.T01:CDS
TGAAAGTTGATGAAGTTGTT+TGG 0.470795 6:-114101929 None:intergenic
ATCCACTTCTTACTGATGGT+TGG 0.473720 6:+114102227 MsG0680035896.01.T01:CDS
AATTCTTAATCCTAAGAAGA+AGG 0.474339 6:+114102751 MsG0680035896.01.T01:CDS
ACTTCATCAACTTTCACCTA+TGG 0.475861 6:+114101936 MsG0680035896.01.T01:CDS
TTGTTGTCTTCAAAGAAAAG+TGG 0.498344 6:-114102124 None:intergenic
CCTATTGCCGTCTTCATGGA+GGG 0.526335 6:-114102659 None:intergenic
GGGGTGGAAGGAGTTCTGTA+AGG 0.538186 6:+114102685 MsG0680035896.01.T01:CDS
GGCAATAGGAGTGGGGTGGA+AGG 0.540633 6:+114102673 MsG0680035896.01.T01:CDS
ACTTCTTACTGATGGTTGGA+AGG 0.546167 6:+114102231 MsG0680035896.01.T01:CDS
ATGAAGACGGCAATAGGAGT+GGG 0.547210 6:+114102665 MsG0680035896.01.T01:CDS
ATTCCACGACTATGGTAAGT+AGG 0.548596 6:-114102367 None:intergenic
TGATGGTTGGAAGGATATGC+AGG 0.555282 6:+114102240 MsG0680035896.01.T01:CDS
TTCCAACCATCAGTAAGAAG+TGG 0.557115 6:-114102229 None:intergenic
CATAGTCGTGGAATGAGAGT+TGG 0.563775 6:+114102376 MsG0680035896.01.T01:CDS
TTGACTCTGTGTTGCGACAA+TGG 0.565518 6:+114102599 MsG0680035896.01.T01:CDS
TCCTATTGCCGTCTTCATGG+AGG 0.571158 6:-114102660 None:intergenic
AACCCTCTATCTTTCTGGCA+AGG 0.572362 6:+114102005 MsG0680035896.01.T01:CDS
TGGTAAGTAGGAATCTGATC+CGG 0.572710 6:-114102355 None:intergenic
AGGGTTGTAAAACCTCCATA+TGG 0.588874 6:-114101988 None:intergenic
AGTAGAAGAGAACAACCTCC+TGG 0.595482 6:-114102285 None:intergenic
CCTCAAGGTTACTTTCAACA+AGG 0.595975 6:+114102195 MsG0680035896.01.T01:CDS
CTTGATGAACAACCATATGG+AGG 0.601812 6:+114101976 MsG0680035896.01.T01:CDS
AAATTCCAATAATTCTCCAT+AGG 0.603347 6:-114101952 None:intergenic
CATGAAGACGGCAATAGGAG+TGG 0.603758 6:+114102664 MsG0680035896.01.T01:CDS
GGATGAACCCTCCATGAAGA+CGG 0.619923 6:+114102652 MsG0680035896.01.T01:CDS
ATTCCTACTTACCATAGTCG+TGG 0.621922 6:+114102364 MsG0680035896.01.T01:CDS
AAACTAACTAACCAAATGAG+AGG 0.627212 6:-114102446 None:intergenic
TACCTTGCCAGAAAGATAGA+GGG 0.634498 6:-114102007 None:intergenic
AGACGGCAATAGGAGTGGGG+TGG 0.655910 6:+114102669 MsG0680035896.01.T01:CDS
CCTTGTTGAAAGTAACCTTG+AGG 0.679764 6:-114102195 None:intergenic
TGAAGACGGCAATAGGAGTG+GGG 0.695742 6:+114102666 MsG0680035896.01.T01:CDS
CTACGCGGATAAGAGCCTCA+AGG 0.697712 6:+114102180 MsG0680035896.01.T01:CDS
CAACTGGAGATAGAAATGCA+TGG 0.726975 6:-114102404 None:intergenic
ATGGACGATAATGAACTACG+CGG 0.807507 6:+114102165 MsG0680035896.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAACAAATCACAAAACA+TGG - Chr6:114102062-114102081 None:intergenic 20.0%
! AAATTCCAATAATTCTCCAT+AGG - Chr6:114101955-114101974 None:intergenic 25.0%
! AATTCTTAATCCTAAGAAGA+AGG + Chr6:114102751-114102770 MsG0680035896.01.T01:CDS 25.0%
AAAACATGAACCTTCTTCTT+AGG - Chr6:114102764-114102783 None:intergenic 30.0%
AAACTAACTAACCAAATGAG+AGG - Chr6:114102449-114102468 None:intergenic 30.0%
TTGTTGTCTTCAAAGAAAAG+TGG - Chr6:114102127-114102146 None:intergenic 30.0%
TTTCACCTATGGAGAATTAT+TGG + Chr6:114101947-114101966 MsG0680035896.01.T01:CDS 30.0%
! TTTTGTTCAGAGAATCCATA+GGG + Chr6:114102538-114102557 MsG0680035896.01.T01:intron 30.0%
!! ACTTTTCTTTGAAGACAACA+AGG + Chr6:114102126-114102145 MsG0680035896.01.T01:CDS 30.0%
!! GAAGAAGGTTCATGTTTTTA+AGG + Chr6:114102766-114102785 MsG0680035896.01.T01:CDS 30.0%
!! TCATTATCGTCCATTTTTCA+GGG - Chr6:114102159-114102178 None:intergenic 30.0%
!! TGAAAGTTGATGAAGTTGTT+TGG - Chr6:114101932-114101951 None:intergenic 30.0%
!! TTCATTATCGTCCATTTTTC+AGG - Chr6:114102160-114102179 None:intergenic 30.0%
!!! AGGTTCATGTTTTTAAGGTT+AGG + Chr6:114102771-114102790 MsG0680035896.01.T01:CDS 30.0%
!!! CTGTAAGGAAATTGGTTTTA+AGG + Chr6:114102700-114102719 MsG0680035896.01.T01:CDS 30.0%
AAGGAGTTCTGTAAGGAAAT+TGG + Chr6:114102692-114102711 MsG0680035896.01.T01:CDS 35.0%
ACTTCATCAACTTTCACCTA+TGG + Chr6:114101936-114101955 MsG0680035896.01.T01:CDS 35.0%
TAGTAAGAAGCTCGAAAATC+CGG + Chr6:114102336-114102355 MsG0680035896.01.T01:CDS 35.0%
TTCCTTGATGAACAACCATA+TGG + Chr6:114101973-114101992 MsG0680035896.01.T01:CDS 35.0%
TTTACAACCCTCTATCTTTC+TGG + Chr6:114102000-114102019 MsG0680035896.01.T01:CDS 35.0%
! AAGGATATGCAGGATGTTTT+TGG + Chr6:114102250-114102269 MsG0680035896.01.T01:CDS 35.0%
! ACTATGGTAACGATCTCTTT+GGG + Chr6:114102305-114102324 MsG0680035896.01.T01:CDS 35.0%
! CCAAAATCCTGCTGATTAAT+CGG - Chr6:114102094-114102113 None:intergenic 35.0%
! GAAAACCTGAACACTCTTTT+TGG - Chr6:114102627-114102646 None:intergenic 35.0%
! TACTATGGTAACGATCTCTT+TGG + Chr6:114102304-114102323 MsG0680035896.01.T01:CDS 35.0%
!! CAACAGTAAAAGCATTCAAC+TGG - Chr6:114102423-114102442 None:intergenic 35.0%
!! GTTTTGTTCAGAGAATCCAT+AGG + Chr6:114102537-114102556 MsG0680035896.01.T01:intron 35.0%
ACTTCTTACTGATGGTTGGA+AGG + Chr6:114102231-114102250 MsG0680035896.01.T01:CDS 40.0%
AGGATGATCTCCCTGAAAAA+TGG + Chr6:114102146-114102165 MsG0680035896.01.T01:CDS 40.0%
AGGGTTGTAAAACCTCCATA+TGG - Chr6:114101991-114102010 None:intergenic 40.0%
ATCCACTTCTTACTGATGGT+TGG + Chr6:114102227-114102246 MsG0680035896.01.T01:CDS 40.0%
ATGGACGATAATGAACTACG+CGG + Chr6:114102165-114102184 MsG0680035896.01.T01:CDS 40.0%
ATTCCACGACTATGGTAAGT+AGG - Chr6:114102370-114102389 None:intergenic 40.0%
ATTCCTACTTACCATAGTCG+TGG + Chr6:114102364-114102383 MsG0680035896.01.T01:CDS 40.0%
CAACTGGAGATAGAAATGCA+TGG - Chr6:114102407-114102426 None:intergenic 40.0%
CCTTGTTGAAAGTAACCTTG+AGG - Chr6:114102198-114102217 None:intergenic 40.0%
CTCCATATGGTTGTTCATCA+AGG - Chr6:114101978-114101997 None:intergenic 40.0%
CTTGATGAACAACCATATGG+AGG + Chr6:114101976-114101995 MsG0680035896.01.T01:CDS 40.0%
GAGGTTGTTCTCTTCTACTA+TGG + Chr6:114102289-114102308 MsG0680035896.01.T01:CDS 40.0%
GTTCATCCACTTCTTACTGA+TGG + Chr6:114102223-114102242 MsG0680035896.01.T01:CDS 40.0%
TACCTTGCCAGAAAGATAGA+GGG - Chr6:114102010-114102029 None:intergenic 40.0%
TCAGTAAGAAGTGGATGAAC+AGG - Chr6:114102223-114102242 None:intergenic 40.0%
TGGTAAGTAGGAATCTGATC+CGG - Chr6:114102358-114102377 None:intergenic 40.0%
TTACCTTGCCAGAAAGATAG+AGG - Chr6:114102011-114102030 None:intergenic 40.0%
TTCCAACCATCAGTAAGAAG+TGG - Chr6:114102232-114102251 None:intergenic 40.0%
TTTCTGACCGATTAATCAGC+AGG + Chr6:114102084-114102103 MsG0680035896.01.T01:intron 40.0%
! CCGATTAATCAGCAGGATTT+TGG + Chr6:114102091-114102110 MsG0680035896.01.T01:intron 40.0%
! CCTCAAGGTTACTTTCAACA+AGG + Chr6:114102195-114102214 MsG0680035896.01.T01:CDS 40.0%
!! GAGTGTTCAGGTTTTCTATG+TGG + Chr6:114102631-114102650 MsG0680035896.01.T01:CDS 40.0%
!! TAATCAGCAGGATTTTGGAG+AGG + Chr6:114102096-114102115 MsG0680035896.01.T01:intron 40.0%
!!! TTTTGGTTTTCACCGAAACC+AGG + Chr6:114102267-114102286 MsG0680035896.01.T01:CDS 40.0%
AGAGAACAACCTCCTGGTTT+CGG - Chr6:114102282-114102301 None:intergenic 45.0%
AGTAGAAGAGAACAACCTCC+TGG - Chr6:114102288-114102307 None:intergenic 45.0%
ATGAAGACGGCAATAGGAGT+GGG + Chr6:114102665-114102684 MsG0680035896.01.T01:CDS 45.0%
CAACTCTCATTCCACGACTA+TGG - Chr6:114102378-114102397 None:intergenic 45.0%
CATAGTCCTCAAACTCCCTA+TGG - Chr6:114102556-114102575 None:intergenic 45.0%
CATAGTCGTGGAATGAGAGT+TGG + Chr6:114102376-114102395 MsG0680035896.01.T01:CDS 45.0%
GAGAATCCATAGGGAGTTTG+AGG + Chr6:114102547-114102566 MsG0680035896.01.T01:intron 45.0%
GAGGACTATGTTAGAGCTTG+TGG + Chr6:114102566-114102585 MsG0680035896.01.T01:CDS 45.0%
TACTGTTGCTGCCTCTCATT+TGG + Chr6:114102435-114102454 MsG0680035896.01.T01:CDS 45.0%
TGATGGTTGGAAGGATATGC+AGG + Chr6:114102240-114102259 MsG0680035896.01.T01:CDS 45.0%
TTGACTCTGTGTTGCGACAA+TGG + Chr6:114102599-114102618 MsG0680035896.01.T01:CDS 45.0%
! AACCCTCTATCTTTCTGGCA+AGG + Chr6:114102005-114102024 MsG0680035896.01.T01:CDS 45.0%
!! TGGTGCCAAAAAGAGTGTTC+AGG + Chr6:114102619-114102638 MsG0680035896.01.T01:CDS 45.0%
CACTCCTATTGCCGTCTTCA+TGG - Chr6:114102666-114102685 None:intergenic 50.0%
CATGAAGACGGCAATAGGAG+TGG + Chr6:114102664-114102683 MsG0680035896.01.T01:CDS 50.0%
CCTATTGCCGTCTTCATGGA+GGG - Chr6:114102662-114102681 None:intergenic 50.0%
GGATGAACCCTCCATGAAGA+CGG + Chr6:114102652-114102671 MsG0680035896.01.T01:CDS 50.0%
TCCTATTGCCGTCTTCATGG+AGG - Chr6:114102663-114102682 None:intergenic 50.0%
TGAAGACGGCAATAGGAGTG+GGG + Chr6:114102666-114102685 MsG0680035896.01.T01:CDS 50.0%
! TGGTTTTCACCGAAACCAGG+AGG + Chr6:114102270-114102289 MsG0680035896.01.T01:CDS 50.0%
CCCTCCATGAAGACGGCAAT+AGG + Chr6:114102659-114102678 MsG0680035896.01.T01:CDS 55.0%
CTACGCGGATAAGAGCCTCA+AGG + Chr6:114102180-114102199 MsG0680035896.01.T01:CDS 55.0%
GGGGTGGAAGGAGTTCTGTA+AGG + Chr6:114102685-114102704 MsG0680035896.01.T01:CDS 55.0%
AGACGGCAATAGGAGTGGGG+TGG + Chr6:114102669-114102688 MsG0680035896.01.T01:CDS 60.0%
GGCAATAGGAGTGGGGTGGA+AGG + Chr6:114102673-114102692 MsG0680035896.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr6 gene 114101904 114102799 114101904 ID=MsG0680035896.01;Name=MsG0680035896.01
Chr6 mRNA 114101904 114102799 114101904 ID=MsG0680035896.01.T01;Parent=MsG0680035896.01;Name=MsG0680035896.01.T01;_AED=0.50;_eAED=0.78;_QI=0|0|0|1|1|1|3|0|241
Chr6 exon 114101904 114102026 114101904 ID=MsG0680035896.01.T01:exon:10054;Parent=MsG0680035896.01.T01
Chr6 exon 114102106 114102456 114102106 ID=MsG0680035896.01.T01:exon:10055;Parent=MsG0680035896.01.T01
Chr6 exon 114102548 114102799 114102548 ID=MsG0680035896.01.T01:exon:10056;Parent=MsG0680035896.01.T01
Chr6 CDS 114101904 114102026 114101904 ID=MsG0680035896.01.T01:cds;Parent=MsG0680035896.01.T01
Chr6 CDS 114102106 114102456 114102106 ID=MsG0680035896.01.T01:cds;Parent=MsG0680035896.01.T01
Chr6 CDS 114102548 114102799 114102548 ID=MsG0680035896.01.T01:cds;Parent=MsG0680035896.01.T01
Gene Sequence

>MsG0680035896.01.T01

ATGGACAACATGCGTATTATTTTCACCAAACAACTTCATCAACTTTCACCTATGGAGAATTATTGGAATTTCCTTGATGAACAACCATATGGAGGTTTTACAACCCTCTATCTTTCTGGCAAGGATTTTGGAGAGGTTGATCCACTTTTCTTTGAAGACAACAAGGATGATCTCCCTGAAAAATGGACGATAATGAACTACGCGGATAAGAGCCTCAAGGTTACTTTCAACAAGGATCCTGTTCATCCACTTCTTACTGATGGTTGGAAGGATATGCAGGATGTTTTTGGTTTTCACCGAAACCAGGAGGTTGTTCTCTTCTACTATGGTAACGATCTCTTTGGGATATATCATAGTAAGAAGCTCGAAAATCCGGATCAGATTCCTACTTACCATAGTCGTGGAATGAGAGTTGGACAGACCATGCATTTCTATCTCCAGTTGAATGCTTTTACTGTTGCTGCCTCTCATTTGAGAATCCATAGGGAGTTTGAGGACTATGTTAGAGCTTGTGGTTTTACTAATTTGACTCTGTGTTGCGACAATGGTGCCAAAAAGAGTGTTCAGGTTTTCTATGTGGATGAACCCTCCATGAAGACGGCAATAGGAGTGGGGTGGAAGGAGTTCTGTAAGGAAATTGGTTTTAAGGATCGTGACATTTTGTGTTTCAAGTTTGAAATTCTTAATCCTAAGAAGAAGGTTCATGTTTTTAAGGTTAGGATGTGA

Protein sequence

>MsG0680035896.01.T01

MDNMRIIFTKQLHQLSPMENYWNFLDEQPYGGFTTLYLSGKDFGEVDPLFFEDNKDDLPEKWTIMNYADKSLKVTFNKDPVHPLLTDGWKDMQDVFGFHRNQEVVLFYYGNDLFGIYHSKKLENPDQIPTYHSRGMRVGQTMHFYLQLNAFTVAASHLRIHREFEDYVRACGFTNLTLCCDNGAKKSVQVFYVDEPSMKTAIGVGWKEFCKEIGFKDRDILCFKFEILNPKKKVHVFKVRM*