AlfalfaGEDB | MsG0380015876.01

M. sativa cultivar ZhongmuNo.1 / MsG0380015876.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015876.01.T01	KAG8655586.1	45.217	115	52	4	1	108	1	111	1.20E-13	77

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015876.01.T01	A0A199UBK9	45.217	115	52	4	1	108	1	111	5.73e-14	77.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000470.01	MsG0380015876.01	0.808643	2.800275e-50	1.609636e-47
MsG0180001892.01	MsG0380015876.01	0.809175	2.154164e-50	1.255964e-47
MsG0180002492.01	MsG0380015876.01	0.805668	1.194722e-49	6.358222e-47
MsG0180004249.01	MsG0380015876.01	0.810930	9.024501e-51	5.512183e-48
MsG0280006324.01	MsG0380015876.01	0.801620	8.263444e-49	3.964332e-46
MsG0380012067.01	MsG0380015876.01	0.806098	9.699562e-50	5.219219e-47
MsG0380015332.01	MsG0380015876.01	0.806782	6.958079e-50	3.810073e-47
MsG0380015875.01	MsG0380015876.01	0.844322	8.123904e-59	1.306720e-55
MsG0380015876.01	MsG0380018005.01	0.805517	1.285068e-49	6.812198e-47
MsG0380015876.01	MsG0480018396.01	0.800051	1.727989e-48	7.967193e-46
MsG0380015876.01	MsG0480018653.01	0.801470	8.870075e-49	4.239390e-46
MsG0380015876.01	MsG0480020836.01	0.801493	8.774420e-49	4.196186e-46
MsG0380015876.01	MsG0480022827.01	0.821484	3.940718e-53	3.210104e-50
MsG0380015876.01	MsG0480023015.01	0.803291	3.739727e-49	1.872144e-46
MsG0380015876.01	MsG0480023202.01	0.803872	2.833691e-49	1.440023e-46
MsG0380015876.01	MsG0580024038.01	0.819964	8.807885e-53	6.878395e-50
MsG0380015876.01	MsG0580026515.01	0.813630	2.322633e-51	1.524716e-48
MsG0380015876.01	MsG0580027925.01	0.809174	2.155994e-50	1.256979e-47
MsG0380015876.01	MsG0680030880.01	0.807386	5.185501e-50	2.884324e-47
MsG0380015876.01	MsG0780037054.01	0.801876	7.321095e-49	3.535442e-46
MsG0380015876.01	MsG0780038232.01	0.804199	2.422399e-49	1.241327e-46
MsG0380015876.01	MsG0780038439.01	0.805140	1.541562e-49	8.093560e-47
MsG0380015876.01	MsG0780039423.01	0.803821	2.903060e-49	1.473308e-46
MsG0380015876.01	MsG0780039443.01	0.812234	4.697791e-51	2.970640e-48
MsG0380015876.01	MsG0780040074.01	0.814056	1.870919e-51	1.242418e-48
MsG0380015876.01	MsG0880043941.01	0.808818	2.568877e-50	1.483464e-47
MsG0280007432.01	MsG0380015876.01	0.800443	1.437879e-48	6.696060e-46
MsG0280007955.01	MsG0380015876.01	0.821105	4.819299e-53	3.884771e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015876.01.T01	MTR_3g079330	43.478	138	46	5	1	107	1	137	3.90e-18	79.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015876.01.T01	AT5G21060	35.762	151	65	6	1	119	1	151	5.08e-14	67.4
MsG0380015876.01.T01	AT5G21060	35.065	154	64	6	1	119	1	153	7.05e-14	67.0
MsG0380015876.01.T01	AT5G21060	35.065	154	64	6	1	119	1	153	7.05e-14	67.0

Find 27 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GACAATTCCTTTGATTCTAA+TGG	0.207940	3:-76197737	MsG0380015876.01.T01:CDS
ACAATTCCTTTGATTCTAAT+GGG	0.346091	3:-76197736	MsG0380015876.01.T01:CDS
CTATCAAAACTTGGTGATCT+TGG	0.347928	3:-76196924	MsG0380015876.01.T01:CDS
ATTGTGTTGTTGTGTTGTTA+TGG	0.378649	3:-76196549	MsG0380015876.01.T01:CDS
GCAGTGGTCTTAGCTTGTTT+TGG	0.382582	3:-76196890	MsG0380015876.01.T01:CDS
GTGAAAGATTAAATTAAAGT+TGG	0.384171	3:+76197794	None:intergenic
TCTTACCATTGTAATGTAAG+AGG	0.410149	3:+76196515	None:intergenic
CTTTGCGGCTCAAGAATTTG+TGG	0.413156	3:-76196954	MsG0380015876.01.T01:CDS
TTTGTGGAAAGTAAGTAGTA+AGG	0.441056	3:+76197863	None:intergenic
AGCTCTTGTTAGAAACTTTG+CGG	0.458678	3:-76196969	MsG0380015876.01.T01:CDS
CTAATGGGTTGTGGAGGAGT+TGG	0.462718	3:-76197721	MsG0380015876.01.T01:CDS
CCTTTGATTCTAATGGGTTG+TGG	0.464584	3:-76197730	MsG0380015876.01.T01:CDS
GGCGAATCTCTATCAAAACT+TGG	0.521436	3:-76196933	MsG0380015876.01.T01:CDS
ATAATGGAAGAAGCAGTTTG+TGG	0.529193	3:+76197847	None:intergenic
CCACAACCCATTAGAATCAA+AGG	0.529303	3:+76197730	None:intergenic
TGCTAGCGCTGACACTATAG+TGG	0.538497	3:-76196590	MsG0380015876.01.T01:CDS
GCCGTTCTCTCACTCTTCAC+AGG	0.544212	3:-76197669	MsG0380015876.01.T01:intron
TTGATTCTAATGGGTTGTGG+AGG	0.548675	3:-76197727	MsG0380015876.01.T01:CDS
TTGTGGAAAGTAAGTAGTAA+GGG	0.557846	3:+76197864	None:intergenic
AACGGCACGAGACAATGTGT+TGG	0.574800	3:+76197686	None:intergenic
ACCTGTGAAGAGTGAGAGAA+CGG	0.598591	3:+76197668	None:intergenic
AGTGGTACTTAAACAAGTGG+TGG	0.609746	3:-76196572	MsG0380015876.01.T01:CDS
AAAAGCCTCTTACATTACAA+TGG	0.612103	3:-76196520	MsG0380015876.01.T01:CDS
TATAGTGGTACTTAAACAAG+TGG	0.613328	3:-76196575	MsG0380015876.01.T01:CDS
ACGAGACAATGTGTTGGAGA+AGG	0.615279	3:+76197692	None:intergenic
GCTAGCAGTGCAATCTACAA+AGG	0.654213	3:+76196606	None:intergenic
CTTGGTAGATATCTACGCAG+TGG	0.685714	3:-76196906	MsG0380015876.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTATGGATTTTATGAAA+TGG	+	Chr3:76196559-76196578	None:intergenic	15.0%
!!!	TCATCTTTTTGAAATTTTAT+TGG	-	Chr3:76197164-76197183	MsG0380015876.01.T01:intron	15.0%
!!!	TTGTTTGTTTTTTTTATCAT+GGG	-	Chr3:76196993-76197012	MsG0380015876.01.T01:intron	15.0%
!!!	TTTGTTTGTTTTTTTTATCA+TGG	-	Chr3:76196992-76197011	MsG0380015876.01.T01:intron	15.0%
!!	AAAATTATCTAGAAGACAAA+TGG	+	Chr3:76196971-76196990	None:intergenic	20.0%
!!	AAATTATCTAGAAGACAAAT+GGG	+	Chr3:76196970-76196989	None:intergenic	20.0%
!!	GTGAAAGATTAAATTAAAGT+TGG	+	Chr3:76196588-76196607	None:intergenic	20.0%
!!	TTGTTGAGATTATTGAATAA+GGG	-	Chr3:76197377-76197396	MsG0380015876.01.T01:intron	20.0%
!!!	AAAGTTGGAAATTGATTTTA+TGG	+	Chr3:76196573-76196592	None:intergenic	20.0%
!!!	AAATTTTTAAGCTGTTTTTG+TGG	-	Chr3:76197269-76197288	MsG0380015876.01.T01:intron	20.0%
!!!	ATGAAATTCTACTCTTATTT+AGG	+	Chr3:76197712-76197731	None:intergenic	20.0%
!!!	GAATTTGATTGTTATTTGAT+TGG	-	Chr3:76197301-76197320	MsG0380015876.01.T01:intron	20.0%
!!!	GATTTTATGAAATGGAATAA+TGG	+	Chr3:76196551-76196570	None:intergenic	20.0%
!	ACAATTCCTTTGATTCTAAT+GGG	-	Chr3:76196643-76196662	MsG0380015876.01.T01:intron	25.0%
!	ACTCTTTGATTGTAATTGAA+AGG	-	Chr3:76197548-76197567	MsG0380015876.01.T01:intron	25.0%
!	CCAAAGAATAGATGAATTAA+TGG	+	Chr3:76197092-76197111	None:intergenic	25.0%
!	CCATTAATTCATCTATTCTT+TGG	-	Chr3:76197089-76197108	MsG0380015876.01.T01:intron	25.0%
!	GTTGTTGAGATTATTGAATA+AGG	-	Chr3:76197376-76197395	MsG0380015876.01.T01:intron	25.0%
!	TAATTCATCTATTCTTTGGA+AGG	-	Chr3:76197093-76197112	MsG0380015876.01.T01:intron	25.0%
!	TGTTGAGATTATTGAATAAG+GGG	-	Chr3:76197378-76197397	MsG0380015876.01.T01:intron	25.0%
!	TTTGATACTAAAACGCAAAT+GGG	+	Chr3:76196899-76196918	None:intergenic	25.0%
!!	CAATAGCTAGATGAATTTTA+AGG	+	Chr3:76197044-76197063	None:intergenic	25.0%
!!	TTTTGATACTAAAACGCAAA+TGG	+	Chr3:76196900-76196919	None:intergenic	25.0%
!!!	AATCTCTAAAATCTTTCCTT+CGG	+	Chr3:76197603-76197622	None:intergenic	25.0%
	AAAAGCCTCTTACATTACAA+TGG	-	Chr3:76197859-76197878	MsG0380015876.01.T01:five_prime_UTR	30.0%
	GACAATTCCTTTGATTCTAA+TGG	-	Chr3:76196642-76196661	MsG0380015876.01.T01:intron	30.0%
	TATAGTGGTACTTAAACAAG+TGG	-	Chr3:76197804-76197823	MsG0380015876.01.T01:five_prime_UTR	30.0%
	TCTTACCATTGTAATGTAAG+AGG	+	Chr3:76197867-76197886	None:intergenic	30.0%
	TTGTGGAAAGTAAGTAGTAA+GGG	+	Chr3:76196518-76196537	None:intergenic	30.0%
	TTTGTGGAAAGTAAGTAGTA+AGG	+	Chr3:76196519-76196538	None:intergenic	30.0%
!	ATTGTGTTGTTGTGTTGTTA+TGG	-	Chr3:76197830-76197849	MsG0380015876.01.T01:five_prime_UTR	30.0%
!	TTTTGATACTAGAAGGCAAA+TGG	+	Chr3:76196828-76196847	None:intergenic	30.0%
!!	GCAATGATTTTGATACTAGA+AGG	+	Chr3:76196835-76196854	None:intergenic	30.0%
!!	TGATTGTTATTTGATTGGCA+GGG	-	Chr3:76197306-76197325	MsG0380015876.01.T01:intron	30.0%
!!	TTGATTGTTATTTGATTGGC+AGG	-	Chr3:76197305-76197324	MsG0380015876.01.T01:intron	30.0%
!!!	AGACTTTTTCATTGTAGGTT+TGG	-	Chr3:76197750-76197769	MsG0380015876.01.T01:exon	30.0%
!!!	TAGACAGACTTTTTCATTGT+AGG	-	Chr3:76197745-76197764	MsG0380015876.01.T01:CDS	30.0%
	AAAATCTTTCCTTCGGATTC+CGG	+	Chr3:76197596-76197615	None:intergenic	35.0%
	AATCACACATGTTAGTGTCA+CGG	+	Chr3:76197131-76197150	None:intergenic	35.0%
	AGCTCTTGTTAGAAACTTTG+CGG	-	Chr3:76197410-76197429	MsG0380015876.01.T01:intron	35.0%
	GAGATTATTGAATAAGGGGT+TGG	-	Chr3:76197382-76197401	MsG0380015876.01.T01:intron	35.0%
	TCTCAACTTGGTAAAAAGAC+AGG	-	Chr3:76197626-76197645	MsG0380015876.01.T01:intron	35.0%
	TTAGAGATTGCATCTCAACT+TGG	-	Chr3:76197614-76197633	MsG0380015876.01.T01:intron	35.0%
!	ATAATGGAAGAAGCAGTTTG+TGG	+	Chr3:76196535-76196554	None:intergenic	35.0%
!	CTATCAAAACTTGGTGATCT+TGG	-	Chr3:76197455-76197474	MsG0380015876.01.T01:intron	35.0%
!	TGATCGTTTTCTGACATTGA+TGG	-	Chr3:76197233-76197252	MsG0380015876.01.T01:intron	35.0%
!!	GATTGTTATTTGATTGGCAG+GGG	-	Chr3:76197307-76197326	MsG0380015876.01.T01:intron	35.0%
	AGTGGTACTTAAACAAGTGG+TGG	-	Chr3:76197807-76197826	MsG0380015876.01.T01:five_prime_UTR	40.0%
	ATGTCAAGTATGTGTGCATC+CGG	-	Chr3:76197574-76197593	MsG0380015876.01.T01:intron	40.0%
	CAACTTGGTAAAAAGACAGG+TGG	-	Chr3:76197629-76197648	MsG0380015876.01.T01:intron	40.0%
	CCACAACCCATTAGAATCAA+AGG	+	Chr3:76196652-76196671	None:intergenic	40.0%
	CGATCATACTCTATGAAGCA+CGG	+	Chr3:76197219-76197238	None:intergenic	40.0%
	CTAAAACGCAAATGGGTCAT+CGG	+	Chr3:76196892-76196911	None:intergenic	40.0%
	GGCGAATCTCTATCAAAACT+TGG	-	Chr3:76197446-76197465	MsG0380015876.01.T01:intron	40.0%
!	TATTCTTTGGAAGGCATGTC+TGG	-	Chr3:76197102-76197121	MsG0380015876.01.T01:intron	40.0%
!	TTGGTGATAGTAATCTCTGG+TGG	-	Chr3:76197354-76197373	MsG0380015876.01.T01:intron	40.0%
!!	AGGTTGGTGATAGTAATCTC+TGG	-	Chr3:76197351-76197370	MsG0380015876.01.T01:intron	40.0%
!!	CCTTTGATTCTAATGGGTTG+TGG	-	Chr3:76196649-76196668	MsG0380015876.01.T01:intron	40.0%
!!	TTGATTCTAATGGGTTGTGG+AGG	-	Chr3:76196652-76196671	MsG0380015876.01.T01:intron	40.0%
	ACCTGTGAAGAGTGAGAGAA+CGG	+	Chr3:76196714-76196733	None:intergenic	45.0%
	ACGAGACAATGTGTTGGAGA+AGG	+	Chr3:76196690-76196709	None:intergenic	45.0%
	CTTGGTAGATATCTACGCAG+TGG	-	Chr3:76197473-76197492	MsG0380015876.01.T01:intron	45.0%
	CTTTGCGGCTCAAGAATTTG+TGG	-	Chr3:76197425-76197444	MsG0380015876.01.T01:intron	45.0%
	GCTAGCAGTGCAATCTACAA+AGG	+	Chr3:76197776-76197795	None:intergenic	45.0%
	GGACTATGCTTGAGAGTTGT+GGG	-	Chr3:76197328-76197347	MsG0380015876.01.T01:intron	45.0%
!!!	GCAGTGGTCTTAGCTTGTTT+TGG	-	Chr3:76197489-76197508	MsG0380015876.01.T01:intron	45.0%
	AACGGCACGAGACAATGTGT+TGG	+	Chr3:76196696-76196715	None:intergenic	50.0%
	ACTCTATGAAGCACGGACAC+CGG	+	Chr3:76197212-76197231	None:intergenic	50.0%
	CTATGCTTGAGAGTTGTGGG+AGG	-	Chr3:76197331-76197350	MsG0380015876.01.T01:intron	50.0%
	GGGACTATGCTTGAGAGTTG+TGG	-	Chr3:76197327-76197346	MsG0380015876.01.T01:intron	50.0%
!	TGACCTGTAAGTGTCGTGTC+CGG	-	Chr3:76197190-76197209	MsG0380015876.01.T01:intron	50.0%
!!	CTAATGGGTTGTGGAGGAGT+TGG	-	Chr3:76196658-76196677	MsG0380015876.01.T01:intron	50.0%
!!	TGCTAGCGCTGACACTATAG+TGG	-	Chr3:76197789-76197808	MsG0380015876.01.T01:five_prime_UTR	50.0%
	ACACCGGACACGACACTTAC+AGG	+	Chr3:76197196-76197215	None:intergenic	55.0%
	GCCGTTCTCTCACTCTTCAC+AGG	-	Chr3:76196710-76196729	MsG0380015876.01.T01:intron	55.0%
	GCTTGAGAGTTGTGGGAGGT+TGG	-	Chr3:76197335-76197354	MsG0380015876.01.T01:intron	55.0%
	TGTGTGCATCCGGAATCCGA+AGG	-	Chr3:76197584-76197603	MsG0380015876.01.T01:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	76196495	76197906	76196495	ID=MsG0380015876.01;Name=MsG0380015876.01
Chr3	mRNA	76196495	76197906	76196495	ID=MsG0380015876.01.T01;Parent=MsG0380015876.01;Name=MsG0380015876.01.T01;_AED=0.49;_eAED=0.68;_QI=142\|0\|0.33\|1\|0\|0\|3\|0\|127
Chr3	exon	76196495	76196653	76196495	ID=MsG0380015876.01.T01:exon:1167;Parent=MsG0380015876.01.T01
Chr3	exon	76196874	76197003	76196874	ID=MsG0380015876.01.T01:exon:1166;Parent=MsG0380015876.01.T01
Chr3	exon	76197670	76197906	76197670	ID=MsG0380015876.01.T01:exon:1165;Parent=MsG0380015876.01.T01
Chr3	five_prime_UTR	76197765	76197906	76197765	ID=MsG0380015876.01.T01:five_prime_utr;Parent=MsG0380015876.01.T01
Chr3	CDS	76197670	76197764	76197670	ID=MsG0380015876.01.T01:cds;Parent=MsG0380015876.01.T01
Chr3	CDS	76196874	76197003	76196874	ID=MsG0380015876.01.T01:cds;Parent=MsG0380015876.01.T01
Chr3	CDS	76196495	76196653	76196495	ID=MsG0380015876.01.T01:cds;Parent=MsG0380015876.01.T01

Gene Sequence

>MsG0380015876.01.T01

ATGAAGACAATTCCTTTGATTCTAATGGGTTGTGGAGGAGTTGGTTCTCACCTTCTCCAACACATTGTCTCGTGCCGTTCTCTCACTCTTCACAGGGGTTGGATGATAGCTCTTGTTAGAAACTTTGCGGCTCAAGAATTTGTGGCGAATCTCTATCAAAACTTGGTGATCTTGGTAGATATCTACGCAGTGGTCTTAGCTTGTTTTGGTTGTGATATGAATGTTACAGACTTTTTCATTGTAGGTTTGGCCTTTGTAGATTGCACTGCTAGCGCTGACACTATAGTGGTACTTAAACAAGTGGTGGATTGTGTTGTTGTGTTGTTATGGCAAATAAAAAGCCTCTTACATTACAATGGTAAGATCATCACAATGTGTTTATAA

Protein sequence

>MsG0380015876.01.T01

MKTIPLILMGCGGVGSHLLQHIVSCRSLTLHRGWMIALVRNFAAQEFVANLYQNLVILVDIYAVVLACFGCDMNVTDFFIVGLAFVDCTASADTIVVLKQVVDCVVVLLWQIKSLLHYNGKIITMCL*