Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016818.01.T01 | XP_013468204.1 | 54.331 | 254 | 55 | 4 | 8 | 200 | 146 | 399 | 2.60E-77 | 247 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016818.01.T01 | A0A072VKT8 | 54.331 | 254 | 55 | 4 | 8 | 200 | 146 | 399 | 1.24e-77 | 247 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016818.01.T01 | MTR_1g064570 | 54.331 | 254 | 55 | 4 | 8 | 200 | 146 | 399 | 3.15e-81 | 247 |
| MsG0380016818.01.T01 | MTR_5g038260 | 43.443 | 244 | 82 | 4 | 14 | 201 | 140 | 383 | 3.64e-57 | 185 |
| MsG0380016818.01.T01 | MTR_5g038230 | 44.545 | 220 | 84 | 5 | 14 | 199 | 138 | 353 | 1.51e-52 | 172 |
| MsG0380016818.01.T01 | MTR_6g023955 | 41.624 | 197 | 65 | 5 | 4 | 200 | 63 | 209 | 2.91e-39 | 134 |
| MsG0380016818.01.T01 | MTR_5g082780 | 37.186 | 199 | 75 | 5 | 16 | 168 | 36 | 230 | 8.94e-29 | 107 |
| MsG0380016818.01.T01 | MTR_1g108740 | 35.470 | 234 | 82 | 10 | 14 | 199 | 132 | 344 | 4.02e-26 | 103 |
| MsG0380016818.01.T01 | MTR_7g076100 | 35.802 | 243 | 87 | 9 | 15 | 200 | 69 | 299 | 1.24e-23 | 95.5 |
| MsG0380016818.01.T01 | MTR_7g077620 | 32.231 | 242 | 97 | 8 | 14 | 199 | 140 | 370 | 1.29e-19 | 85.9 |
| MsG0380016818.01.T01 | MTR_7g077660 | 32.377 | 244 | 92 | 10 | 14 | 200 | 140 | 367 | 2.46e-19 | 84.7 |
| MsG0380016818.01.T01 | MTR_1g016150 | 41.935 | 124 | 44 | 4 | 14 | 137 | 141 | 236 | 8.10e-17 | 76.3 |
| MsG0380016818.01.T01 | MTR_7g077650 | 29.839 | 248 | 98 | 9 | 14 | 200 | 139 | 371 | 2.15e-15 | 73.9 |
| MsG0380016818.01.T01 | MTR_7g076110 | 31.020 | 245 | 98 | 11 | 15 | 200 | 80 | 312 | 9.20e-15 | 71.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGTTAAAACTTGATAGTT+TGG | 0.233392 | 3:-88234632 | MsG0380016818.01.T01:CDS |
| TAGTGATTCCTTATCATTAT+CGG | 0.290772 | 3:-88234584 | MsG0380016818.01.T01:CDS |
| CACGAATGCCACCTGCACTA+TGG | 0.300114 | 3:-88234399 | MsG0380016818.01.T01:CDS |
| TTCATATCAAATGGTCCATT+AGG | 0.318173 | 3:+88234481 | None:intergenic |
| TATCAACCAATCCTACAATT+AGG | 0.348220 | 3:-88235067 | MsG0380016818.01.T01:CDS |
| TGAAGTGTATAAGTTGGAAT+TGG | 0.373389 | 3:-88234680 | MsG0380016818.01.T01:CDS |
| TGTTATCCCGAACTTGTTGA+TGG | 0.375401 | 3:-88234745 | MsG0380016818.01.T01:CDS |
| ATGCCACTTCCTTACCCTAA+TGG | 0.387509 | 3:-88234496 | MsG0380016818.01.T01:CDS |
| ATGTTAAAACTTGATAGTTT+GGG | 0.387582 | 3:-88234631 | MsG0380016818.01.T01:CDS |
| AATATTTGAAGTGTATAAGT+TGG | 0.390550 | 3:-88234686 | MsG0380016818.01.T01:CDS |
| TCATCGCCATCAACAAGTTC+GGG | 0.406527 | 3:+88234739 | None:intergenic |
| ATCATCGCCATCAACAAGTT+CGG | 0.408035 | 3:+88234738 | None:intergenic |
| TTTCTCTAATTGCCTACAAA+AGG | 0.412681 | 3:-88234554 | MsG0380016818.01.T01:CDS |
| AGCTATCAATGCCACTGAAA+TGG | 0.414072 | 3:+88234367 | None:intergenic |
| TCATATCAAATGGTCCATTA+GGG | 0.424543 | 3:+88234482 | None:intergenic |
| TAATCATGTGGCCTAATTGT+AGG | 0.430933 | 3:+88235056 | None:intergenic |
| TGTTGGATATTGATAGAAGA+AGG | 0.472402 | 3:-88235124 | MsG0380016818.01.T01:CDS |
| ACGAATGCCACCTGCACTAT+GGG | 0.477124 | 3:-88234398 | MsG0380016818.01.T01:CDS |
| TTATAGATCTTCATATCAAA+TGG | 0.481791 | 3:+88234472 | None:intergenic |
| AAGATCTATAACGTAAAAGA+TGG | 0.483831 | 3:-88234460 | MsG0380016818.01.T01:CDS |
| ATAAGAGCAATGTTGACTAA+AGG | 0.486756 | 3:+88234435 | None:intergenic |
| TTGTGAATGTAATGTGCATA+AGG | 0.487014 | 3:-88235096 | MsG0380016818.01.T01:CDS |
| AACATTGCTCTTATCAACAT+TGG | 0.493940 | 3:-88234426 | MsG0380016818.01.T01:CDS |
| ATATGAAGCCGATAATGATA+AGG | 0.517001 | 3:+88234576 | None:intergenic |
| TCAAATGGTCCATTAGGGTA+AGG | 0.534096 | 3:+88234487 | None:intergenic |
| TAAAGGTCTTGTCTATGCTG+TGG | 0.538623 | 3:-88234921 | MsG0380016818.01.T01:CDS |
| TTGTCTATGCTGTGGGACGA+TGG | 0.542229 | 3:-88234913 | MsG0380016818.01.T01:CDS |
| GGGTTCTTCCTCCATTTCAG+TGG | 0.542520 | 3:-88234378 | MsG0380016818.01.T01:CDS |
| GTTGGATATTGATAGAAGAA+GGG | 0.552961 | 3:-88235123 | MsG0380016818.01.T01:CDS |
| AAAGGTCTTGTCTATGCTGT+GGG | 0.553289 | 3:-88234920 | MsG0380016818.01.T01:CDS |
| ATGTGGCCTAATTGTAGGAT+TGG | 0.559107 | 3:+88235061 | None:intergenic |
| GGAGGAAGAACCCATAGTGC+AGG | 0.568705 | 3:+88234388 | None:intergenic |
| TTGGAATTGGATATTCAAAG+TGG | 0.583810 | 3:-88234667 | MsG0380016818.01.T01:CDS |
| TATCAATGCCACTGAAATGG+AGG | 0.597336 | 3:+88234370 | None:intergenic |
| GGTCCATTAGGGTAAGGAAG+TGG | 0.598558 | 3:+88234493 | None:intergenic |
| GATAGATTCATCCATAGTAG+TGG | 0.613859 | 3:-88234715 | MsG0380016818.01.T01:CDS |
| GGAAGAACCCATAGTGCAGG+TGG | 0.627590 | 3:+88234391 | None:intergenic |
| GCTACAACAACATAATCATG+TGG | 0.663446 | 3:+88235044 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACAGATGTTATATTTTATAA+AGG | - | Chr3:88234559-88234578 | MsG0380016818.01.T01:CDS | 15.0% |
| !! | TTATAGATCTTCATATCAAA+TGG | + | Chr3:88235028-88235047 | None:intergenic | 20.0% |
| !!! | AATATTTGAAGTGTATAAGT+TGG | - | Chr3:88234811-88234830 | MsG0380016818.01.T01:intron | 20.0% |
| !!! | AATGTTAAAACTTGATAGTT+TGG | - | Chr3:88234865-88234884 | MsG0380016818.01.T01:intron | 20.0% |
| !!! | ATGTTAAAACTTGATAGTTT+GGG | - | Chr3:88234866-88234885 | MsG0380016818.01.T01:intron | 20.0% |
| ! | AAGATCTATAACGTAAAAGA+TGG | - | Chr3:88235037-88235056 | MsG0380016818.01.T01:CDS | 25.0% |
| ! | TAGTGATTCCTTATCATTAT+CGG | - | Chr3:88234913-88234932 | MsG0380016818.01.T01:CDS | 25.0% |
| !! | AATAGATTGAATCCTTTTGT+AGG | + | Chr3:88234958-88234977 | None:intergenic | 25.0% |
| !! | TATAAGTCCAAATTTTTGTC+CGG | + | Chr3:88234517-88234536 | None:intergenic | 25.0% |
| !!! | ATATTTTAGTACCACTACTA+TGG | + | Chr3:88234796-88234815 | None:intergenic | 25.0% |
| AACATTGCTCTTATCAACAT+TGG | - | Chr3:88235071-88235090 | MsG0380016818.01.T01:CDS | 30.0% | |
| ATAAAAGCCGGACAAAAATT+TGG | - | Chr3:88234507-88234526 | MsG0380016818.01.T01:CDS | 30.0% | |
| ATATGAAGCCGATAATGATA+AGG | + | Chr3:88234924-88234943 | None:intergenic | 30.0% | |
| GTTGGATATTGATAGAAGAA+GGG | - | Chr3:88234374-88234393 | MsG0380016818.01.T01:CDS | 30.0% | |
| TATCAACCAATCCTACAATT+AGG | - | Chr3:88234430-88234449 | MsG0380016818.01.T01:CDS | 30.0% | |
| TCATATCAAATGGTCCATTA+GGG | + | Chr3:88235018-88235037 | None:intergenic | 30.0% | |
| TGTTGGATATTGATAGAAGA+AGG | - | Chr3:88234373-88234392 | MsG0380016818.01.T01:CDS | 30.0% | |
| TTCATATCAAATGGTCCATT+AGG | + | Chr3:88235019-88235038 | None:intergenic | 30.0% | |
| TTGGAATTGGATATTCAAAG+TGG | - | Chr3:88234830-88234849 | MsG0380016818.01.T01:intron | 30.0% | |
| TTGTGAATGTAATGTGCATA+AGG | - | Chr3:88234401-88234420 | MsG0380016818.01.T01:CDS | 30.0% | |
| TTTCTCTAATTGCCTACAAA+AGG | - | Chr3:88234943-88234962 | MsG0380016818.01.T01:intron | 30.0% | |
| ! | ATAAGAGCAATGTTGACTAA+AGG | + | Chr3:88235065-88235084 | None:intergenic | 30.0% |
| ! | TGAAGTGTATAAGTTGGAAT+TGG | - | Chr3:88234817-88234836 | MsG0380016818.01.T01:intron | 30.0% |
| !! | GGAGACAATGTTTTATTCTT+GGG | - | Chr3:88234887-88234906 | MsG0380016818.01.T01:intron | 30.0% |
| AGGAATTACCTTCTCATTCT+TGG | + | Chr3:88234641-88234660 | None:intergenic | 35.0% | |
| GATAGATTCATCCATAGTAG+TGG | - | Chr3:88234782-88234801 | MsG0380016818.01.T01:intron | 35.0% | |
| GCTACAACAACATAATCATG+TGG | + | Chr3:88234456-88234475 | None:intergenic | 35.0% | |
| TAATCATGTGGCCTAATTGT+AGG | + | Chr3:88234444-88234463 | None:intergenic | 35.0% | |
| TATCTTGTGAAATCGTTGGA+GGG | - | Chr3:88234702-88234721 | MsG0380016818.01.T01:CDS | 35.0% | |
| TCCTAATGTTCTTTCTTCCT+GGG | - | Chr3:88234657-88234676 | MsG0380016818.01.T01:CDS | 35.0% | |
| TGTCTTGCTTTCATAAAAGC+CGG | - | Chr3:88234495-88234514 | MsG0380016818.01.T01:CDS | 35.0% | |
| TTATCTTGTGAAATCGTTGG+AGG | - | Chr3:88234701-88234720 | MsG0380016818.01.T01:CDS | 35.0% | |
| TTCCTAATGTTCTTTCTTCC+TGG | - | Chr3:88234656-88234675 | MsG0380016818.01.T01:CDS | 35.0% | |
| !! | GGGAGACAATGTTTTATTCT+TGG | - | Chr3:88234886-88234905 | MsG0380016818.01.T01:intron | 35.0% |
| ATCATCGCCATCAACAAGTT+CGG | + | Chr3:88234762-88234781 | None:intergenic | 40.0% | |
| ATGTGGCCTAATTGTAGGAT+TGG | + | Chr3:88234439-88234458 | None:intergenic | 40.0% | |
| CCTAATGTTCTTTCTTCCTG+GGG | - | Chr3:88234658-88234677 | MsG0380016818.01.T01:CDS | 40.0% | |
| CTAATGTTCTTTCTTCCTGG+GGG | - | Chr3:88234659-88234678 | MsG0380016818.01.T01:CDS | 40.0% | |
| GGCTTATCTTGTGAAATCGT+TGG | - | Chr3:88234698-88234717 | MsG0380016818.01.T01:CDS | 40.0% | |
| TCAAATGGTCCATTAGGGTA+AGG | + | Chr3:88235013-88235032 | None:intergenic | 40.0% | |
| ! | AAAGGTCTTGTCTATGCTGT+GGG | - | Chr3:88234577-88234596 | MsG0380016818.01.T01:CDS | 40.0% |
| ! | GTGTTACTCCAAGAATGAGA+AGG | - | Chr3:88234630-88234649 | MsG0380016818.01.T01:CDS | 40.0% |
| ! | TAAAGGTCTTGTCTATGCTG+TGG | - | Chr3:88234576-88234595 | MsG0380016818.01.T01:CDS | 40.0% |
| !! | TGTTATCCCGAACTTGTTGA+TGG | - | Chr3:88234752-88234771 | MsG0380016818.01.T01:CDS | 40.0% |
| CCCCAGGAAGAAAGAACATT+AGG | + | Chr3:88234661-88234680 | None:intergenic | 45.0% | |
| TCATCGCCATCAACAAGTTC+GGG | + | Chr3:88234761-88234780 | None:intergenic | 45.0% | |
| ! | ATGCCACTTCCTTACCCTAA+TGG | - | Chr3:88235001-88235020 | MsG0380016818.01.T01:intron | 45.0% |
| AATCGTTGGAGGGTGAGTTG+TGG | - | Chr3:88234712-88234731 | MsG0380016818.01.T01:CDS | 50.0% | |
| ACGAATGCCACCTGCACTAT+GGG | - | Chr3:88235099-88235118 | MsG0380016818.01.T01:CDS | 50.0% | |
| GGGTTCTTCCTCCATTTCAG+TGG | - | Chr3:88235119-88235138 | MsG0380016818.01.T01:CDS | 50.0% | |
| GGTCCATTAGGGTAAGGAAG+TGG | + | Chr3:88235007-88235026 | None:intergenic | 50.0% | |
| TTGTCTATGCTGTGGGACGA+TGG | - | Chr3:88234584-88234603 | MsG0380016818.01.T01:CDS | 50.0% | |
| CACGAATGCCACCTGCACTA+TGG | - | Chr3:88235098-88235117 | MsG0380016818.01.T01:CDS | 55.0% | |
| GGAAGAACCCATAGTGCAGG+TGG | + | Chr3:88235109-88235128 | None:intergenic | 55.0% | |
| GGAGGAAGAACCCATAGTGC+AGG | + | Chr3:88235112-88235131 | None:intergenic | 55.0% | |
| ! | TGGGGGACTGTTTTGCGCAT+CGG | - | Chr3:88234676-88234695 | MsG0380016818.01.T01:CDS | 55.0% |
| GATGCGCAAAACAGTCCCCC+AGG | + | Chr3:88234677-88234696 | None:intergenic | 60.0% | |
| ! | GGGGGACTGTTTTGCGCATC+GGG | - | Chr3:88234677-88234696 | MsG0380016818.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 88234372 | 88235147 | 88234372 | ID=MsG0380016818.01;Name=MsG0380016818.01 |
| Chr3 | mRNA | 88234372 | 88235147 | 88234372 | ID=MsG0380016818.01.T01;Parent=MsG0380016818.01;Name=MsG0380016818.01.T01;_AED=0.50;_eAED=0.54;_QI=0|0|0|1|0|0|3|0|201 |
| Chr3 | exon | 88235030 | 88235147 | 88235030 | ID=MsG0380016818.01.T01:exon:12197;Parent=MsG0380016818.01.T01 |
| Chr3 | exon | 88234888 | 88234956 | 88234888 | ID=MsG0380016818.01.T01:exon:12196;Parent=MsG0380016818.01.T01 |
| Chr3 | exon | 88234372 | 88234790 | 88234372 | ID=MsG0380016818.01.T01:exon:12195;Parent=MsG0380016818.01.T01 |
| Chr3 | CDS | 88235030 | 88235147 | 88235030 | ID=MsG0380016818.01.T01:cds;Parent=MsG0380016818.01.T01 |
| Chr3 | CDS | 88234888 | 88234956 | 88234888 | ID=MsG0380016818.01.T01:cds;Parent=MsG0380016818.01.T01 |
| Chr3 | CDS | 88234372 | 88234790 | 88234372 | ID=MsG0380016818.01.T01:cds;Parent=MsG0380016818.01.T01 |
| Gene Sequence |
| Protein sequence |
