AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039468.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039468.01.T01 MTR_7g077620 66.220 373 116 7 1 366 1 370 4.26e-161 457
MsG0780039468.01.T01 MTR_7g077650 61.097 383 123 13 1 368 1 372 2.89e-145 416
MsG0780039468.01.T01 MTR_7g077660 61.186 371 131 9 5 368 4 368 8.87e-141 404
MsG0780039468.01.T01 MTR_7g076100 73.418 237 56 4 137 368 66 300 1.85e-112 330
MsG0780039468.01.T01 MTR_7g076100 84.615 52 8 0 1 52 1 52 1.47e-24 102
MsG0780039468.01.T01 MTR_7g076110 58.163 294 112 6 85 368 21 313 8.92e-104 308
MsG0780039468.01.T01 MTR_5g038260 44.000 400 165 16 6 368 6 383 6.05e-84 260
MsG0780039468.01.T01 MTR_1g108740 44.180 378 156 16 6 366 5 344 6.18e-80 248
MsG0780039468.01.T01 MTR_5g038230 42.278 395 149 16 6 366 4 353 3.54e-77 242
MsG0780039468.01.T01 MTR_1g064570 40.380 421 171 17 5 368 3 400 1.24e-72 231
MsG0780039468.01.T01 MTR_5g082770 45.161 310 131 11 6 301 4 288 1.81e-67 214
MsG0780039468.01.T01 MTR_7g076130 70.968 124 32 2 167 286 25 148 7.46e-54 175
MsG0780039468.01.T01 MTR_1g016150 47.208 197 95 5 3 193 2 195 2.34e-48 164
MsG0780039468.01.T01 MTR_1g016150 59.375 64 18 2 254 314 177 235 2.29e-12 66.6
MsG0780039468.01.T01 MTR_5g082780 41.150 226 101 7 112 323 1 208 2.71e-41 145
MsG0780039468.01.T01 MTR_1g108750 44.156 154 52 4 6 158 4 124 8.25e-24 95.5
MsG0780039468.01.T01 MTR_3g114970 23.454 388 232 16 5 364 3 353 1.16e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 70 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTGGTTGGCTTTCATTAA+TGG 0.140857 7:+64538845 MsG0780039468.01.T01:CDS
GCAGCAACGACATAATTATT+TGG 0.166448 7:-64538987 None:intergenic
AAAGAGCAAGCCAACCATTT+TGG 0.203122 7:+64539039 MsG0780039468.01.T01:CDS
AAGTCTTGATGTTTATAAAT+TGG 0.216976 7:+64539334 MsG0780039468.01.T01:CDS
TTCTCATAGTAAGTAAAATT+AGG 0.229231 7:-64539191 None:intergenic
TATCCCATATGAAAATTAAA+TGG 0.231038 7:+64538808 MsG0780039468.01.T01:CDS
TAATTATTTGGGCTTGTAAT+GGG 0.291906 7:-64538975 None:intergenic
GGTATACTACTACACTTAAA+AGG 0.314179 7:-64538897 None:intergenic
CAGCAACGACATAATTATTT+GGG 0.330157 7:-64538986 None:intergenic
TATGTCGTTGCTGCCATTTA+CGG 0.330732 7:+64538996 MsG0780039468.01.T01:CDS
AAGATGAGAAAATCCGTAAA+TGG 0.376143 7:-64539009 None:intergenic
AAGAACACATCGATCACATT+AGG 0.376838 7:+64538598 MsG0780039468.01.T01:CDS
CGCATCCCCTTGAAGGAAAT+AGG 0.376883 7:-64539578 None:intergenic
AGAAGAACTACCTCTTAGTT+TGG 0.384813 7:+64538560 MsG0780039468.01.T01:CDS
TCTCATAGTAAGTAAAATTA+GGG 0.387493 7:-64539190 None:intergenic
ATCTTTACCTATTTCCTTCA+AGG 0.391074 7:+64539571 MsG0780039468.01.T01:CDS
AAAACCTCGCATCCCCTTGA+AGG 0.402319 7:-64539585 None:intergenic
TTTGAGAGGTGGCAGACTAA+TGG 0.407722 7:-64538922 None:intergenic
GGGTTCTACCGCAATTTCAA+TGG 0.408089 7:+64539609 MsG0780039468.01.T01:CDS
TGATGTTTATAAATTGGAAC+TGG 0.417875 7:+64539340 MsG0780039468.01.T01:CDS
TTGAGAGGTGGCAGACTAAT+GGG 0.429349 7:-64538921 None:intergenic
TATGAAAATTAAATGGTGTA+TGG 0.432693 7:+64538815 MsG0780039468.01.T01:CDS
ATAATTATTTGGGCTTGTAA+TGG 0.437481 7:-64538976 None:intergenic
TTAGGTTTGGTGTGGTGTGC+AGG 0.455358 7:+64538616 MsG0780039468.01.T01:CDS
CACATCGATCACATTAGGTT+TGG 0.455542 7:+64538603 MsG0780039468.01.T01:CDS
AAAGCCTTTCATCATAGCAT+TGG 0.456480 7:-64539244 None:intergenic
AAGCCTTTCATCATAGCATT+GGG 0.457571 7:-64539243 None:intergenic
AAGAGGTAGTTCTTCTAAGT+TGG 0.460578 7:-64538553 None:intergenic
GTATGGACTATTCATTTGGT+TGG 0.468308 7:+64538832 MsG0780039468.01.T01:CDS
TGGTGTATGGACTATTCATT+TGG 0.472010 7:+64538828 MsG0780039468.01.T01:CDS
AATGAGGAAGGTAGTAGATT+GGG 0.475711 7:+64538530 None:intergenic
TAGTTTGGTTCTTGATAAGT+TGG 0.478359 7:+64538575 MsG0780039468.01.T01:CDS
GGTTCTACCGCAATTTCAAT+GGG 0.481590 7:+64539610 MsG0780039468.01.T01:CDS
CTATATGAATCCAAAATGGT+TGG 0.483203 7:-64539049 None:intergenic
CAATGAGGAAGGTAGTAGAT+TGG 0.484957 7:+64538529 None:intergenic
AGGAGGATTGTTGATCTTGA+AGG 0.506138 7:-64539150 None:intergenic
ATACAAGGATCTCTTCTCGT+TGG 0.507143 7:-64538748 None:intergenic
AATCTTTGAATGGAGACATA+TGG 0.507395 7:+64539276 MsG0780039468.01.T01:CDS
GAGATCTATAATGTGAAGGA+TGG 0.509549 7:+64539533 MsG0780039468.01.T01:CDS
CTGCTAATTGCTGATGGCAG+CGG 0.512572 7:+64539113 MsG0780039468.01.T01:CDS
ACCTCTCAAATCTGTTTACA+AGG 0.516115 7:+64538935 MsG0780039468.01.T01:CDS
TTATCAAGAACCAAACTAAG+AGG 0.520565 7:-64538570 None:intergenic
TCTTTACCTATTTCCTTCAA+GGG 0.520742 7:+64539572 MsG0780039468.01.T01:CDS
TTGGTGTGGTGTGCAGGAGT+TGG 0.525759 7:+64538622 MsG0780039468.01.T01:CDS
TACTACTACACTTAAAAGGA+TGG 0.531503 7:-64538893 None:intergenic
CATCCCAATGCTATGATGAA+AGG 0.550966 7:+64539240 MsG0780039468.01.T01:CDS
TTCTAACTTGGAGAAACAAG+AGG 0.552906 7:-64539459 None:intergenic
ATAAATTGGAACTGGATGCC+CGG 0.559787 7:+64539348 MsG0780039468.01.T01:CDS
GTTGATCTTGAAGGATACAA+TGG 0.562836 7:-64539141 None:intergenic
TGTAATGGGGTCAGCAGACA+AGG 0.568289 7:-64538961 None:intergenic
CTTTACCTATTTCCTTCAAG+GGG 0.577911 7:+64539573 MsG0780039468.01.T01:CDS
AATTATTTGGGCTTGTAATG+GGG 0.583882 7:-64538974 None:intergenic
AAAGGTCTGCTAATTGCTGA+TGG 0.586662 7:+64539107 MsG0780039468.01.T01:CDS
GTGACTTAGAATGCTATACA+AGG 0.596070 7:-64538763 None:intergenic
GTGAGGATCATGAGCATAGG+TGG 0.597353 7:-64538696 None:intergenic
TATCAATCCCATTGAAATTG+CGG 0.597459 7:-64539617 None:intergenic
CTTAGGCTTAGCAATGAGGA+AGG 0.598112 7:+64538518 None:intergenic
CTTTCATCATAGCATTGGGA+TGG 0.603237 7:-64539239 None:intergenic
TTGTAAACAGATTTGAGAGG+TGG 0.604090 7:-64538933 None:intergenic
CGATCACATTAGGTTTGGTG+TGG 0.607424 7:+64538608 MsG0780039468.01.T01:CDS
ATTTCCTTCAAGGGGATGCG+AGG 0.609973 7:+64539581 MsG0780039468.01.T01:CDS
GTTAGAGATCTATAATGTGA+AGG 0.621618 7:+64539529 MsG0780039468.01.T01:CDS
TTCGAGACAGTTCTTCTGTG+AGG 0.625233 7:-64538713 None:intergenic
TTGGAACTGGATGCCCGGAG+TGG 0.634177 7:+64539353 MsG0780039468.01.T01:CDS
TCTGTGAGGATCATGAGCAT+AGG 0.639271 7:-64538699 None:intergenic
GGGTCATATGAGTCAGCACA+AGG 0.657673 7:-64539170 None:intergenic
TTTGAATGGAGACATATGGA+TGG 0.693585 7:+64539280 MsG0780039468.01.T01:CDS
GCCTTGTAAACAGATTTGAG+AGG 0.700590 7:-64538936 None:intergenic
AGAGGTGGCAGACTAATGGG+AGG 0.724272 7:-64538918 None:intergenic
TCATATGAGTCAGCACAAGG+AGG 0.731539 7:-64539167 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGATGTTGTATTTTATAA+AGG + Chr7:64539089-64539108 MsG0780039468.01.T01:CDS 15.0%
!! AAGTCTTGATGTTTATAAAT+TGG + Chr7:64539334-64539353 MsG0780039468.01.T01:CDS 20.0%
!! ACCATAAAAATCATCAATTT+AGG + Chr7:64538664-64538683 MsG0780039468.01.T01:CDS 20.0%
!! TATCCCATATGAAAATTAAA+TGG + Chr7:64538808-64538827 MsG0780039468.01.T01:CDS 20.0%
!! TATGAAAATTAAATGGTGTA+TGG + Chr7:64538815-64538834 MsG0780039468.01.T01:CDS 20.0%
!! TCTCATAGTAAGTAAAATTA+GGG - Chr7:64539193-64539212 None:intergenic 20.0%
!! TTCTCATAGTAAGTAAAATT+AGG - Chr7:64539194-64539213 None:intergenic 20.0%
!!! AATTGAATCTTTTTCTAACT+TGG - Chr7:64539474-64539493 None:intergenic 20.0%
!!! ACACCATTTAATTTTCATAT+GGG - Chr7:64538814-64538833 None:intergenic 20.0%
!!! TACACCATTTAATTTTCATA+TGG - Chr7:64538815-64538834 None:intergenic 20.0%
!!! TATTTTGTGAAATCTTTGAA+TGG + Chr7:64539266-64539285 MsG0780039468.01.T01:CDS 20.0%
!!! TCCTAAATTGATGATTTTTA+TGG - Chr7:64538668-64538687 None:intergenic 20.0%
!!! TTTTCTATATGAATCCAAAA+TGG - Chr7:64539056-64539075 None:intergenic 20.0%
! ATAATTATTTGGGCTTGTAA+TGG - Chr7:64538979-64538998 None:intergenic 25.0%
! ATATGGGATAAAAATGCAAT+GGG - Chr7:64538798-64538817 None:intergenic 25.0%
! TAATTATTTGGGCTTGTAAT+GGG - Chr7:64538978-64538997 None:intergenic 25.0%
! TGATGTTTATAAATTGGAAC+TGG + Chr7:64539340-64539359 MsG0780039468.01.T01:CDS 25.0%
!!! AAGACAACATTTTATTTGTC+GGG + Chr7:64539411-64539430 MsG0780039468.01.T01:CDS 25.0%
!!! GAAGACAACATTTTATTTGT+CGG + Chr7:64539410-64539429 MsG0780039468.01.T01:CDS 25.0%
AAGATGAGAAAATCCGTAAA+TGG - Chr7:64539012-64539031 None:intergenic 30.0%
AATCTTTGAATGGAGACATA+TGG + Chr7:64539276-64539295 MsG0780039468.01.T01:CDS 30.0%
AATGAAAAAGCTTGAGAGTT+TGG + Chr7:64539388-64539407 MsG0780039468.01.T01:CDS 30.0%
AATTATTTGGGCTTGTAATG+GGG - Chr7:64538977-64538996 None:intergenic 30.0%
ATCTTTACCTATTTCCTTCA+AGG + Chr7:64539571-64539590 MsG0780039468.01.T01:CDS 30.0%
CAGCAACGACATAATTATTT+GGG - Chr7:64538989-64539008 None:intergenic 30.0%
CATATGGGATAAAAATGCAA+TGG - Chr7:64538799-64538818 None:intergenic 30.0%
CTATATGAATCCAAAATGGT+TGG - Chr7:64539052-64539071 None:intergenic 30.0%
GGTATACTACTACACTTAAA+AGG - Chr7:64538900-64538919 None:intergenic 30.0%
GTTAGAGATCTATAATGTGA+AGG + Chr7:64539529-64539548 MsG0780039468.01.T01:CDS 30.0%
TACTACTACACTTAAAAGGA+TGG - Chr7:64538896-64538915 None:intergenic 30.0%
TATGGGATAAAAATGCAATG+GGG - Chr7:64538797-64538816 None:intergenic 30.0%
TCTTTACCTATTTCCTTCAA+GGG + Chr7:64539572-64539591 MsG0780039468.01.T01:CDS 30.0%
TTATCAAGAACCAAACTAAG+AGG - Chr7:64538573-64538592 None:intergenic 30.0%
!! AACATTTTATTTGTCGGGAT+TGG + Chr7:64539416-64539435 MsG0780039468.01.T01:CDS 30.0%
!! ATTTGGTTGGCTTTCATTAA+TGG + Chr7:64538845-64538864 MsG0780039468.01.T01:CDS 30.0%
!! TAGTTTGGTTCTTGATAAGT+TGG + Chr7:64538575-64538594 MsG0780039468.01.T01:CDS 30.0%
AAAGCCTTTCATCATAGCAT+TGG - Chr7:64539247-64539266 None:intergenic 35.0%
AAGAACACATCGATCACATT+AGG + Chr7:64538598-64538617 MsG0780039468.01.T01:CDS 35.0%
AAGAGGTAGTTCTTCTAAGT+TGG - Chr7:64538556-64538575 None:intergenic 35.0%
AAGCCTTTCATCATAGCATT+GGG - Chr7:64539246-64539265 None:intergenic 35.0%
ACCTCTCAAATCTGTTTACA+AGG + Chr7:64538935-64538954 MsG0780039468.01.T01:CDS 35.0%
AGAAGAACTACCTCTTAGTT+TGG + Chr7:64538560-64538579 MsG0780039468.01.T01:CDS 35.0%
CTTTACCTATTTCCTTCAAG+GGG + Chr7:64539573-64539592 MsG0780039468.01.T01:CDS 35.0%
GAGATCTATAATGTGAAGGA+TGG + Chr7:64539533-64539552 MsG0780039468.01.T01:CDS 35.0%
GCAGCAACGACATAATTATT+TGG - Chr7:64538990-64539009 None:intergenic 35.0%
GTGACTTAGAATGCTATACA+AGG - Chr7:64538766-64538785 None:intergenic 35.0%
GTTGATCTTGAAGGATACAA+TGG - Chr7:64539144-64539163 None:intergenic 35.0%
TTCTAACTTGGAGAAACAAG+AGG - Chr7:64539462-64539481 None:intergenic 35.0%
TTGTAAACAGATTTGAGAGG+TGG - Chr7:64538936-64538955 None:intergenic 35.0%
TTTGAATGGAGACATATGGA+TGG + Chr7:64539280-64539299 MsG0780039468.01.T01:CDS 35.0%
! GTATGGACTATTCATTTGGT+TGG + Chr7:64538832-64538851 MsG0780039468.01.T01:CDS 35.0%
! TGGTGTATGGACTATTCATT+TGG + Chr7:64538828-64538847 MsG0780039468.01.T01:CDS 35.0%
!! TTTGTTCAAGTTTTCCACTC+CGG - Chr7:64539370-64539389 None:intergenic 35.0%
AAAGGTCTGCTAATTGCTGA+TGG + Chr7:64539107-64539126 MsG0780039468.01.T01:CDS 40.0%
AGGAGGATTGTTGATCTTGA+AGG - Chr7:64539153-64539172 None:intergenic 40.0%
ATAAATTGGAACTGGATGCC+CGG + Chr7:64539348-64539367 MsG0780039468.01.T01:CDS 40.0%
ATACAAGGATCTCTTCTCGT+TGG - Chr7:64538751-64538770 None:intergenic 40.0%
CACATCGATCACATTAGGTT+TGG + Chr7:64538603-64538622 MsG0780039468.01.T01:CDS 40.0%
CATCCCAATGCTATGATGAA+AGG + Chr7:64539240-64539259 MsG0780039468.01.T01:CDS 40.0%
GCCTTGTAAACAGATTTGAG+AGG - Chr7:64538939-64538958 None:intergenic 40.0%
GGTTCTACCGCAATTTCAAT+GGG + Chr7:64539610-64539629 MsG0780039468.01.T01:CDS 40.0%
! AAAGAGCAAGCCAACCATTT+TGG + Chr7:64539039-64539058 MsG0780039468.01.T01:CDS 40.0%
! CTTTCATCATAGCATTGGGA+TGG - Chr7:64539242-64539261 None:intergenic 40.0%
! TATGTCGTTGCTGCCATTTA+CGG + Chr7:64538996-64539015 MsG0780039468.01.T01:CDS 40.0%
!! TTGTTCAAGTTTTCCACTCC+GGG - Chr7:64539369-64539388 None:intergenic 40.0%
GGGTTCTACCGCAATTTCAA+TGG + Chr7:64539609-64539628 MsG0780039468.01.T01:CDS 45.0%
TCATATGAGTCAGCACAAGG+AGG - Chr7:64539170-64539189 None:intergenic 45.0%
TCTGTGAGGATCATGAGCAT+AGG - Chr7:64538702-64538721 None:intergenic 45.0%
TTCGAGACAGTTCTTCTGTG+AGG - Chr7:64538716-64538735 None:intergenic 45.0%
TTGAGAGGTGGCAGACTAAT+GGG - Chr7:64538924-64538943 None:intergenic 45.0%
TTTGAGAGGTGGCAGACTAA+TGG - Chr7:64538925-64538944 None:intergenic 45.0%
!! CGATCACATTAGGTTTGGTG+TGG + Chr7:64538608-64538627 MsG0780039468.01.T01:CDS 45.0%
AAAACCTCGCATCCCCTTGA+AGG - Chr7:64539588-64539607 None:intergenic 50.0%
ATTTCCTTCAAGGGGATGCG+AGG + Chr7:64539581-64539600 MsG0780039468.01.T01:CDS 50.0%
CGCATCCCCTTGAAGGAAAT+AGG - Chr7:64539581-64539600 None:intergenic 50.0%
CTGCTAATTGCTGATGGCAG+CGG + Chr7:64539113-64539132 MsG0780039468.01.T01:CDS 50.0%
GGGTCATATGAGTCAGCACA+AGG - Chr7:64539173-64539192 None:intergenic 50.0%
GTGAGGATCATGAGCATAGG+TGG - Chr7:64538699-64538718 None:intergenic 50.0%
! CAAGGGGATGCGAGGTTTTT+GGG + Chr7:64539589-64539608 MsG0780039468.01.T01:CDS 50.0%
! TCAAGGGGATGCGAGGTTTT+TGG + Chr7:64539588-64539607 MsG0780039468.01.T01:CDS 50.0%
! TGTAATGGGGTCAGCAGACA+AGG - Chr7:64538964-64538983 None:intergenic 50.0%
!! TTAGGTTTGGTGTGGTGTGC+AGG + Chr7:64538616-64538635 MsG0780039468.01.T01:CDS 50.0%
AGAGGTGGCAGACTAATGGG+AGG - Chr7:64538921-64538940 None:intergenic 55.0%
TTGGTGTGGTGTGCAGGAGT+TGG + Chr7:64538622-64538641 MsG0780039468.01.T01:CDS 55.0%
TTGGAACTGGATGCCCGGAG+TGG + Chr7:64539353-64539372 MsG0780039468.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 64538531 64539637 64538531 ID=MsG0780039468.01;Name=MsG0780039468.01
Chr7 mRNA 64538531 64539637 64538531 ID=MsG0780039468.01.T01;Parent=MsG0780039468.01;Name=MsG0780039468.01.T01;_AED=0.31;_eAED=0.31;_QI=0|-1|0|1|-1|1|1|0|368
Chr7 exon 64538531 64539637 64538531 ID=MsG0780039468.01.T01:exon:9891;Parent=MsG0780039468.01.T01
Chr7 CDS 64538531 64539637 64538531 ID=MsG0780039468.01.T01:cds;Parent=MsG0780039468.01.T01
Gene Sequence

>MsG0780039468.01.T01

ATGAGGAAGGTAGTAGATTGGGCCAACTTAGAAGAACTACCTCTTAGTTTGGTTCTTGATAAGTTGGAAGAACACATCGATCACATTAGGTTTGGTGTGGTGTGCAGGAGTTGGCTATCTGTTGCAAAGCTTAACCATAAAAATCATCAATTTAGGATTAGTATACCACCTATGCTCATGATCCTCACAGAAGAACTGTCTCGAAGACGTAAAAAAACCAACGAGAAGAGATCCTTGTATAGCATTCTAAGTCACAAAGAGTACCCCATTGCATTTTTATCCCATATGAAAATTAAATGGTGTATGGACTATTCATTTGGTTGGCTTTCATTAATGGACTATAACAATGTCATAACTCTATTCCATCCTTTTAAGTGTAGTAGTATACCTCCCATTAGTCTGCCACCTCTCAAATCTGTTTACAAGGCTACCTTGTCTGCTGACCCCATTACAAGCCCAAATAATTATGTCGTTGCTGCCATTTACGGATTTTCTCATCTTGCATTCAAAAGAGCAAGCCAACCATTTTGGATTCATATAGAAAATGAATGTGCTATCATTGATGTTGTATTTTATAAAGGTCTGCTAATTGCTGATGGCAGCGGAAAAACCATTGTATCCTTCAAGATCAACAATCCTCCTTGTGCTGACTCATATGACCCTAATTTTACTTACTATGAGAAGATAGCTAGCACTAGATATAGTGTTCCATCCCAATGCTATGATGAAAGGCTTTATTTTGTGAAATCTTTGAATGGAGACATATGGATGGTGAGAAGATATTTAAATATCGTTTTAGATGAAAGTCTTGATGTTTATAAATTGGAACTGGATGCCCGGAGTGGAAAACTTGAACAAATGAAAAAGCTTGAGAGTTTGGAAGACAACATTTTATTTGTCGGGATTGGTGATTCAATTTCTGTGTCAGCCTCTTGTTTCTCCAAGTTAGAAAAAGATTCAATTTATTTTATTTATGACAATGATATGCATGATTTAGCGTTAGAGATCTATAATGTGAAGGATGGAAGCTGTCAGAGACAATCTTTACCTATTTCCTTCAAGGGGATGCGAGGTTTTTGGGTTCTACCGCAATTTCAATGGGATTGA
Protein sequence

>MsG0780039468.01.T01

MRKVVDWANLEELPLSLVLDKLEEHIDHIRFGVVCRSWLSVAKLNHKNHQFRISIPPMLMILTEELSRRRKKTNEKRSLYSILSHKEYPIAFLSHMKIKWCMDYSFGWLSLMDYNNVITLFHPFKCSSIPPISLPPLKSVYKATLSADPITSPNNYVVAAIYGFSHLAFKRASQPFWIHIENECAIIDVVFYKGLLIADGSGKTIVSFKINNPPCADSYDPNFTYYEKIASTRYSVPSQCYDERLYFVKSLNGDIWMVRRYLNIVLDESLDVYKLELDARSGKLEQMKKLESLEDNILFVGIGDSISVSASCFSKLEKDSIYFIYDNDMHDLALEIYNVKDGSCQRQSLPISFKGMRGFWVLPQFQWD*