AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047289.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047289.01.T01 MTR_4g124370 97.101 69 2 0 3 71 2 70 4.09e-46 148
MsG0880047289.01.T01 MTR_4g124370 97.101 69 2 0 3 71 2 70 4.12e-46 148
MsG0880047289.01.T01 MTR_4g124370 97.101 69 2 0 3 71 2 70 4.74e-46 148
MsG0880047289.01.T01 MTR_4g124370 97.101 69 2 0 3 71 2 70 4.97e-46 148
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATTAGTATATAAGAAAT+TGG 0.152950 8:+84214203 MsG0880047289.01.T01:five_prime_UTR
CAACACATCGTGCTGCTTCT+TGG 0.262025 8:-84214285 None:intergenic
ATGTAGGCTTTACCACAATT+TGG 0.291221 8:+84214799 MsG0880047289.01.T01:intron
GTTGCAGTTTCAACACCTTC+AGG 0.398198 8:+84214314 MsG0880047289.01.T01:CDS
AAATTAGTATATAAGAAATT+GGG 0.445778 8:+84214204 MsG0880047289.01.T01:five_prime_UTR
CGAACATTCATCGGATCAAA+TGG 0.459353 8:+84214695 MsG0880047289.01.T01:CDS
AGCATCATTGTCGTTGTTGT+GGG 0.498777 8:+84214658 MsG0880047289.01.T01:intron
CAGCATCATTGTCGTTGTTG+TGG 0.502093 8:+84214657 MsG0880047289.01.T01:intron
TCATTGCTTCATTGAAGAAG+AGG 0.508340 8:-84214126 None:intergenic
TTTCAACACCTTCAGGAGAC+GGG 0.531812 8:+84214321 MsG0880047289.01.T01:CDS
CATCGTGCTGCTTCTTGGAA+AGG 0.541545 8:-84214280 None:intergenic
ATTGTGCAGCGAACATTCAT+CGG 0.567403 8:+84214686 MsG0880047289.01.T01:CDS
GTTTCAACACCTTCAGGAGA+CGG 0.574200 8:+84214320 MsG0880047289.01.T01:CDS
GCTGCTTCTTGGAAAGGAGG+AGG 0.581803 8:-84214274 None:intergenic
CGTGCTGCTTCTTGGAAAGG+AGG 0.583012 8:-84214277 None:intergenic
AAAGCATACCCGTCTCCTGA+AGG 0.594623 8:-84214329 None:intergenic
TTCGAGTAAATGCCAAATTG+TGG 0.602887 8:-84214811 None:intergenic
CAAATCATTGCAGAGTACAA+AGG 0.608841 8:-84214155 None:intergenic
CTGATTGTTTCAACAACTCG+CGG 0.618861 8:+84214848 MsG0880047289.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAGTATATAAGAAAT+TGG + Chr8:84214203-84214222 MsG0880047289.01.T01:five_prime_UTR 10.0%
!! AAATTAGTATATAAGAAATT+GGG + Chr8:84214204-84214223 MsG0880047289.01.T01:five_prime_UTR 10.0%
!! TATTCTTAACTGATAAATTT+GGG - Chr8:84214443-84214462 None:intergenic 15.0%
!! TTATTCTTAACTGATAAATT+TGG - Chr8:84214444-84214463 None:intergenic 15.0%
!! TTTGATTATTCAAATTTGAT+CGG + Chr8:84214386-84214405 MsG0880047289.01.T01:intron 15.0%
!!! AATTTTTGTTTTTTAACTTC+AGG + Chr8:84214722-84214741 MsG0880047289.01.T01:intron 15.0%
!!! ATTATGTTTGTAAATTTTAC+TGG + Chr8:84214590-84214609 MsG0880047289.01.T01:intron 15.0%
!!! ATTTTTGTTTTTTAACTTCA+GGG + Chr8:84214723-84214742 MsG0880047289.01.T01:intron 15.0%
!! AGTTAGACTAAAATTTGTAT+TGG + Chr8:84214512-84214531 MsG0880047289.01.T01:intron 20.0%
!!! TGACATTTTCAACAAAATTA+GGG - Chr8:84214239-84214258 None:intergenic 20.0%
!!! TTGACATTTTCAACAAAATT+AGG - Chr8:84214240-84214259 None:intergenic 20.0%
! AAAATCATCTTATGCATGAA+TGG + Chr8:84214561-84214580 MsG0880047289.01.T01:intron 25.0%
! ATCAAATCAAATTCCGAAAT+TGG - Chr8:84214372-84214391 None:intergenic 25.0%
! ATCTTCTTCATTTCCAATTT+CGG + Chr8:84214356-84214375 MsG0880047289.01.T01:intron 25.0%
! CATCACTATAAAATTCATCA+AGG - Chr8:84214770-84214789 None:intergenic 25.0%
! TTATTCAAATTTGATCGGAT+CGG + Chr8:84214391-84214410 MsG0880047289.01.T01:intron 25.0%
!!! AACTGATAAATTTGGGATTT+TGG - Chr8:84214436-84214455 None:intergenic 25.0%
!!! AGTGATGTTTTTTTGTATGT+AGG + Chr8:84214783-84214802 MsG0880047289.01.T01:intron 25.0%
TAACTTCAGGGTAATATTAG+TGG + Chr8:84214735-84214754 MsG0880047289.01.T01:intron 30.0%
! CTATACTGTATACTTTTGTG+TGG + Chr8:84214620-84214639 MsG0880047289.01.T01:intron 30.0%
ATGTAGGCTTTACCACAATT+TGG + Chr8:84214799-84214818 MsG0880047289.01.T01:intron 35.0%
CAAATCATTGCAGAGTACAA+AGG - Chr8:84214158-84214177 None:intergenic 35.0%
TCATTGCTTCATTGAAGAAG+AGG - Chr8:84214129-84214148 None:intergenic 35.0%
TTCGAGTAAATGCCAAATTG+TGG - Chr8:84214814-84214833 None:intergenic 35.0%
AGCATCATTGTCGTTGTTGT+GGG + Chr8:84214658-84214677 MsG0880047289.01.T01:intron 40.0%
ATTGTGCAGCGAACATTCAT+CGG + Chr8:84214686-84214705 MsG0880047289.01.T01:CDS 40.0%
CGAACATTCATCGGATCAAA+TGG + Chr8:84214695-84214714 MsG0880047289.01.T01:CDS 40.0%
CTGATTGTTTCAACAACTCG+CGG + Chr8:84214848-84214867 MsG0880047289.01.T01:CDS 40.0%
CAGCATCATTGTCGTTGTTG+TGG + Chr8:84214657-84214676 MsG0880047289.01.T01:intron 45.0%
GTTGCAGTTTCAACACCTTC+AGG + Chr8:84214314-84214333 MsG0880047289.01.T01:CDS 45.0%
GTTTCAACACCTTCAGGAGA+CGG + Chr8:84214320-84214339 MsG0880047289.01.T01:CDS 45.0%
TTTCAACACCTTCAGGAGAC+GGG + Chr8:84214321-84214340 MsG0880047289.01.T01:CDS 45.0%
AAAGCATACCCGTCTCCTGA+AGG - Chr8:84214332-84214351 None:intergenic 50.0%
!! CAACACATCGTGCTGCTTCT+TGG - Chr8:84214288-84214307 None:intergenic 50.0%
!! CATCGTGCTGCTTCTTGGAA+AGG - Chr8:84214283-84214302 None:intergenic 50.0%
GCTGCTTCTTGGAAAGGAGG+AGG - Chr8:84214277-84214296 None:intergenic 55.0%
!! CGTGCTGCTTCTTGGAAAGG+AGG - Chr8:84214280-84214299 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 84214109 84214873 84214109 ID=MsG0880047289.01;Name=MsG0880047289.01
Chr8 mRNA 84214109 84214873 84214109 ID=MsG0880047289.01.T01;Parent=MsG0880047289.01;Name=MsG0880047289.01.T01;_AED=0.48;_eAED=0.48;_QI=144|1|0.66|1|0.5|0.66|3|0|71
Chr8 exon 84214109 84214342 84214109 ID=MsG0880047289.01.T01:exon:35916;Parent=MsG0880047289.01.T01
Chr8 exon 84214660 84214716 84214660 ID=MsG0880047289.01.T01:exon:35917;Parent=MsG0880047289.01.T01
Chr8 exon 84214805 84214873 84214805 ID=MsG0880047289.01.T01:exon:35918;Parent=MsG0880047289.01.T01
Chr8 five_prime_UTR 84214109 84214252 84214109 ID=MsG0880047289.01.T01:five_prime_utr;Parent=MsG0880047289.01.T01
Chr8 CDS 84214253 84214342 84214253 ID=MsG0880047289.01.T01:cds;Parent=MsG0880047289.01.T01
Chr8 CDS 84214660 84214716 84214660 ID=MsG0880047289.01.T01:cds;Parent=MsG0880047289.01.T01
Chr8 CDS 84214805 84214873 84214805 ID=MsG0880047289.01.T01:cds;Parent=MsG0880047289.01.T01
Gene Sequence

>MsG0880047289.01.T01

ATGTCAACAACAACAGAAACTCCTCCTCCTTTCCAAGAAGCAGCACGATGTGTTGTCTGCAGTTGCAGTTTCAACACCTTCAGGAGACGGCATCATTGTCGTTGTTGTGGGAGAACATTGTGCAGCGAACATTCATCGGATCAAATGGCTTTACCACAATTTGGCATTTACTCGAATGTACGTGTATGTGCTGATTGTTTCAACAACTCGCGGTAA

Protein sequence

>MsG0880047289.01.T01

MSTTTETPPPFQEAARCVVCSCSFNTFRRRHHCRCCGRTLCSEHSSDQMALPQFGIYSNVRVCADCFNNSR*