Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019643.01.T01 | XP_013455329.1 | 97.537 | 203 | 5 | 0 | 1 | 203 | 1 | 203 | 1.04E-140 | 401 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019643.01.T01 | P17407 | 37.736 | 159 | 98 | 1 | 42 | 199 | 27 | 185 | 1.40E-28 | 108 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019643.01.T01 | A0A072UU39 | 97.537 | 203 | 5 | 0 | 1 | 203 | 1 | 203 | 4.99e-141 | 401 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003235.01 | MsG0480019643.01 | 0.808276 | 3.353603e-50 | 1.909468e-47 |
| MsG0180005542.01 | MsG0480019643.01 | 0.819947 | 8.887218e-53 | 6.936915e-50 |
| MsG0280011165.01 | MsG0480019643.01 | 0.824215 | 9.105471e-54 | 8.014228e-51 |
| MsG0280011193.01 | MsG0480019643.01 | 0.813061 | 3.097549e-51 | 2.002125e-48 |
| MsG0280011203.01 | MsG0480019643.01 | 0.807204 | 5.666009e-50 | 3.136756e-47 |
| MsG0380013272.01 | MsG0480019643.01 | 0.811812 | 5.805251e-51 | 3.629199e-48 |
| MsG0480019643.01 | MsG0480020784.01 | 0.850611 | 1.517997e-60 | 2.990204e-57 |
| MsG0480019643.01 | MsG0480023267.01 | 0.800994 | 1.109851e-48 | 5.241638e-46 |
| MsG0480019643.01 | MsG0580024220.01 | 0.806046 | 9.948916e-50 | 5.346314e-47 |
| MsG0480019643.01 | MsG0580024408.01 | 0.832920 | 7.178389e-56 | 8.139195e-53 |
| MsG0480019643.01 | MsG0780036661.01 | 0.825167 | 5.434458e-54 | 4.915597e-51 |
| MsG0480019643.01 | MsG0780039978.01 | 0.817779 | 2.762162e-52 | 2.030514e-49 |
| MsG0480019643.01 | MsG0780040806.01 | 0.823390 | 1.421442e-53 | 1.222103e-50 |
| MsG0480019643.01 | MsG0780041556.01 | 0.800086 | 1.699460e-48 | 7.842827e-46 |
| MsG0480019643.01 | MsG0880045458.01 | 0.807825 | 4.183268e-50 | 2.353574e-47 |
| MsG0480019643.01 | MsG0880046863.01 | 0.804802 | 1.813180e-49 | 9.437643e-47 |
| MsG0480019643.01 | MsG0880046996.01 | 0.801551 | 8.537249e-49 | 4.088605e-46 |
| MsG0380015369.01 | MsG0480019643.01 | 0.814294 | 1.658127e-51 | 1.108275e-48 |
| MsG0380016801.01 | MsG0480019643.01 | 0.800875 | 1.173972e-48 | 5.527425e-46 |
| MsG0380016839.01 | MsG0480019643.01 | 0.815578 | 8.605557e-52 | 5.955935e-49 |
| MsG0280007216.01 | MsG0480019643.01 | 0.801427 | 9.050315e-49 | 4.320874e-46 |
| MsG0280009167.01 | MsG0480019643.01 | 0.827859 | 1.239644e-54 | 1.211586e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019643.01.T01 | MTR_4g035855 | 97.537 | 203 | 5 | 0 | 1 | 203 | 1 | 203 | 1.27e-144 | 401 |
| MsG0480019643.01.T01 | MTR_3g008760 | 40.223 | 179 | 101 | 3 | 29 | 203 | 51 | 227 | 5.80e-37 | 128 |
| MsG0480019643.01.T01 | MTR_4g035870 | 36.735 | 196 | 113 | 4 | 8 | 202 | 8 | 193 | 5.01e-33 | 117 |
| MsG0480019643.01.T01 | MTR_4g035880 | 36.923 | 195 | 112 | 4 | 8 | 201 | 8 | 192 | 6.21e-33 | 117 |
| MsG0480019643.01.T01 | MTR_4g035905 | 34.872 | 195 | 116 | 3 | 8 | 201 | 8 | 192 | 4.61e-31 | 112 |
| MsG0480019643.01.T01 | MTR_8g104765 | 34.762 | 210 | 124 | 6 | 1 | 199 | 1 | 208 | 6.86e-29 | 107 |
| MsG0480019643.01.T01 | MTR_4g120825 | 33.333 | 210 | 118 | 6 | 1 | 199 | 1 | 199 | 2.68e-26 | 100 |
| MsG0480019643.01.T01 | MTR_4g101760 | 29.016 | 193 | 130 | 2 | 8 | 199 | 10 | 196 | 5.59e-26 | 99.8 |
| MsG0480019643.01.T01 | MTR_4g120830 | 32.857 | 210 | 119 | 6 | 1 | 199 | 1 | 199 | 7.76e-26 | 99.4 |
| MsG0480019643.01.T01 | MTR_5g012000 | 29.319 | 191 | 123 | 2 | 12 | 201 | 28 | 207 | 3.51e-24 | 95.5 |
| MsG0480019643.01.T01 | MTR_3g008840 | 32.919 | 161 | 102 | 3 | 43 | 200 | 25 | 182 | 2.09e-21 | 87.4 |
| MsG0480019643.01.T01 | MTR_3g008820 | 31.902 | 163 | 101 | 4 | 43 | 200 | 24 | 181 | 2.68e-19 | 81.6 |
| MsG0480019643.01.T01 | MTR_2g043560 | 31.138 | 167 | 93 | 6 | 43 | 201 | 34 | 186 | 1.08e-15 | 72.8 |
| MsG0480019643.01.T01 | MTR_4g025610 | 27.411 | 197 | 127 | 6 | 9 | 197 | 5 | 193 | 3.18e-15 | 71.6 |
| MsG0480019643.01.T01 | MTR_4g025550 | 26.396 | 197 | 129 | 6 | 9 | 197 | 5 | 193 | 1.78e-14 | 69.7 |
| MsG0480019643.01.T01 | MTR_4g025620 | 26.396 | 197 | 129 | 6 | 9 | 197 | 5 | 193 | 2.96e-14 | 68.9 |
| MsG0480019643.01.T01 | MTR_0036s0140 | 31.098 | 164 | 94 | 4 | 44 | 203 | 78 | 226 | 2.11e-13 | 67.0 |
| MsG0480019643.01.T01 | MTR_8g023310 | 29.630 | 162 | 109 | 3 | 44 | 202 | 68 | 227 | 4.17e-12 | 64.7 |
| MsG0480019643.01.T01 | MTR_4g025590 | 26.380 | 163 | 111 | 5 | 43 | 197 | 3 | 164 | 1.32e-11 | 61.2 |
| MsG0480019643.01.T01 | MTR_4g124855 | 26.977 | 215 | 117 | 7 | 2 | 201 | 7 | 196 | 1.33e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019643.01.T01 | AT5G51520 | 35.849 | 212 | 119 | 4 | 1 | 203 | 1 | 204 | 1.18e-34 | 122 |
| MsG0480019643.01.T01 | AT2G01610 | 38.596 | 171 | 91 | 3 | 43 | 199 | 44 | 214 | 7.80e-31 | 112 |
| MsG0480019643.01.T01 | AT1G14890 | 34.653 | 202 | 121 | 4 | 1 | 199 | 19 | 212 | 2.32e-30 | 111 |
| MsG0480019643.01.T01 | AT4G25260 | 34.359 | 195 | 121 | 5 | 7 | 199 | 6 | 195 | 3.85e-29 | 107 |
| MsG0480019643.01.T01 | AT4G25250 | 32.990 | 194 | 125 | 3 | 8 | 198 | 2 | 193 | 1.44e-28 | 106 |
| MsG0480019643.01.T01 | AT1G62770 | 34.031 | 191 | 119 | 5 | 16 | 201 | 7 | 195 | 3.63e-28 | 105 |
| MsG0480019643.01.T01 | AT1G62760 | 39.873 | 158 | 92 | 2 | 43 | 199 | 147 | 302 | 3.34e-27 | 105 |
| MsG0480019643.01.T01 | AT5G62360 | 35.000 | 160 | 101 | 2 | 42 | 201 | 42 | 198 | 2.02e-26 | 100 |
| MsG0480019643.01.T01 | AT1G70720 | 36.250 | 160 | 97 | 3 | 43 | 200 | 36 | 192 | 7.57e-24 | 94.0 |
| MsG0480019643.01.T01 | AT5G62350 | 36.875 | 160 | 97 | 3 | 43 | 199 | 38 | 196 | 9.69e-24 | 94.0 |
| MsG0480019643.01.T01 | AT1G70720 | 35.625 | 160 | 98 | 2 | 43 | 200 | 48 | 204 | 1.24e-23 | 94.0 |
| MsG0480019643.01.T01 | AT4G12390 | 32.716 | 162 | 106 | 2 | 43 | 201 | 39 | 200 | 1.59e-23 | 93.6 |
| MsG0480019643.01.T01 | AT5G20740 | 30.583 | 206 | 114 | 7 | 7 | 201 | 6 | 193 | 1.96e-22 | 90.5 |
| MsG0480019643.01.T01 | AT5G20740 | 30.583 | 206 | 114 | 7 | 7 | 201 | 40 | 227 | 6.50e-22 | 90.1 |
| MsG0480019643.01.T01 | AT3G47380 | 31.443 | 194 | 125 | 5 | 12 | 199 | 5 | 196 | 4.01e-20 | 84.3 |
| MsG0480019643.01.T01 | AT1G23205 | 30.622 | 209 | 125 | 5 | 1 | 200 | 1 | 198 | 3.03e-17 | 77.0 |
| MsG0480019643.01.T01 | AT2G47670 | 29.213 | 178 | 119 | 2 | 28 | 203 | 33 | 205 | 4.22e-15 | 71.2 |
| MsG0480019643.01.T01 | AT3G14300 | 33.520 | 179 | 99 | 6 | 34 | 201 | 449 | 618 | 9.30e-15 | 72.8 |
| MsG0480019643.01.T01 | AT3G14300 | 28.646 | 192 | 108 | 5 | 30 | 201 | 252 | 434 | 7.79e-13 | 67.0 |
| MsG0480019643.01.T01 | AT3G14300 | 30.539 | 167 | 98 | 5 | 44 | 201 | 77 | 234 | 2.77e-11 | 62.4 |
| MsG0480019643.01.T01 | AT1G53830 | 31.892 | 185 | 120 | 4 | 22 | 202 | 38 | 220 | 1.62e-14 | 71.6 |
| MsG0480019643.01.T01 | AT3G62820 | 25.758 | 198 | 123 | 4 | 9 | 201 | 11 | 189 | 5.38e-13 | 65.1 |
| MsG0480019643.01.T01 | AT4G00080 | 27.692 | 195 | 129 | 6 | 11 | 198 | 7 | 196 | 3.47e-12 | 63.2 |
| MsG0480019643.01.T01 | AT1G53840 | 30.769 | 195 | 102 | 6 | 23 | 201 | 58 | 235 | 5.53e-12 | 64.3 |
| MsG0480019643.01.T01 | AT3G14310 | 30.337 | 178 | 119 | 4 | 28 | 202 | 42 | 217 | 2.05e-11 | 62.8 |
Find 31 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCATCATGTGTTAGCTTCT+TGG | 0.192985 | 4:+23967535 | None:intergenic |
| CTAATGATGATAAGTTTAAA+TGG | 0.212944 | 4:-23967415 | MsG0480019643.01.T01:CDS |
| AGCTAGATATATAGAGATTT+TGG | 0.223149 | 4:+23967604 | None:intergenic |
| CATTTAAAACCATGATTAAT+AGG | 0.235676 | 4:+23967226 | None:intergenic |
| AGCACTCATCCATGTCTTTA+TGG | 0.257024 | 4:+23967388 | None:intergenic |
| TAAGAGTTGAGAGGAATAGA+AGG | 0.274709 | 4:+23967789 | None:intergenic |
| TGAGTGTCATTTGGTTTGTT+TGG | 0.332747 | 4:+23967627 | None:intergenic |
| AGTGACTTGTAGCAATCATT+TGG | 0.363713 | 4:+23967671 | None:intergenic |
| TTCCTTGACAAGCAATGCTT+TGG | 0.406266 | 4:-23967267 | MsG0480019643.01.T01:CDS |
| TACGGATGAGTTGGATGAAA+TGG | 0.428692 | 4:-23967342 | MsG0480019643.01.T01:CDS |
| GTGACTTGTAGCAATCATTT+GGG | 0.434175 | 4:+23967672 | None:intergenic |
| GTAGCAATCATTTGGGTATG+TGG | 0.476953 | 4:+23967679 | None:intergenic |
| TAATGATGATAAGTTTAAAT+GGG | 0.477960 | 4:-23967414 | MsG0480019643.01.T01:CDS |
| TACTCGAGCAATCGTCTGAC+GGG | 0.498622 | 4:-23967463 | MsG0480019643.01.T01:CDS |
| GAGATCTTCAATAAAGAAGA+TGG | 0.505339 | 4:-23967315 | MsG0480019643.01.T01:CDS |
| GGTTGTTGAAGTGGACTTTG+TGG | 0.509749 | 4:+23967736 | None:intergenic |
| GACGGGCTCGTGCATTTGAA+TGG | 0.515185 | 4:-23967446 | MsG0480019643.01.T01:CDS |
| TGTTGAAGTGGACTTTGTGG+TGG | 0.524165 | 4:+23967739 | None:intergenic |
| AACTGATGAGGGAACATGTA+CGG | 0.549911 | 4:-23967360 | MsG0480019643.01.T01:CDS |
| AAGAGTTGAGAGGAATAGAA+GGG | 0.556118 | 4:+23967790 | None:intergenic |
| TAGATTGAATAAGAGTTGAG+AGG | 0.562356 | 4:+23967780 | None:intergenic |
| CTACTCGAGCAATCGTCTGA+CGG | 0.571083 | 4:-23967464 | MsG0480019643.01.T01:CDS |
| AAGATGGTCAACACAAGTGT+TGG | 0.572025 | 4:-23967299 | MsG0480019643.01.T01:CDS |
| GCTAACACATGATGAAACAT+TGG | 0.573094 | 4:-23967528 | MsG0480019643.01.T01:CDS |
| AGCCAAAGCATTGCTTGTCA+AGG | 0.575594 | 4:+23967265 | None:intergenic |
| GGGAACATGTACGGATGAGT+TGG | 0.586993 | 4:-23967351 | MsG0480019643.01.T01:CDS |
| AGTGCTGCTATAACTGATGA+GGG | 0.626850 | 4:-23967371 | MsG0480019643.01.T01:CDS |
| GAGTGCTGCTATAACTGATG+AGG | 0.652188 | 4:-23967372 | MsG0480019643.01.T01:CDS |
| AATGGGATACCATAAAGACA+TGG | 0.682100 | 4:-23967397 | MsG0480019643.01.T01:CDS |
| TAGAGAAAACATCGATGACA+CGG | 0.685307 | 4:-23967492 | MsG0480019643.01.T01:CDS |
| AGAAAACATCGATGACACGG+TGG | 0.757437 | 4:-23967489 | MsG0480019643.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATGATGATAAGTTTAAAT+GGG | - | Chr4:23967657-23967676 | MsG0480019643.01.T01:CDS | 15.0% |
| !! | CTAATGATGATAAGTTTAAA+TGG | - | Chr4:23967656-23967675 | MsG0480019643.01.T01:CDS | 20.0% |
| !! | AGCTAGATATATAGAGATTT+TGG | + | Chr4:23967470-23967489 | None:intergenic | 25.0% |
| !!! | AGTCTTTAAGAACTTTTTGT+AGG | + | Chr4:23967359-23967378 | None:intergenic | 25.0% |
| GAGATCTTCAATAAAGAAGA+TGG | - | Chr4:23967756-23967775 | MsG0480019643.01.T01:CDS | 30.0% | |
| TAGATTGAATAAGAGTTGAG+AGG | + | Chr4:23967294-23967313 | None:intergenic | 30.0% | |
| ! | ATCGATGTTTTCTCTACAAT+CGG | + | Chr4:23967575-23967594 | None:intergenic | 30.0% |
| ! | GTTTGGATTTTTGATGTGTA+TGG | + | Chr4:23967430-23967449 | None:intergenic | 30.0% |
| !!! | ATGCTTTGGCTTTTGTTAAT+AGG | - | Chr4:23967818-23967837 | MsG0480019643.01.T01:CDS | 30.0% |
| !!! | GGAACTTAGTTTTTTCAATG+TGG | + | Chr4:23967518-23967537 | None:intergenic | 30.0% |
| AAGAGTTGAGAGGAATAGAA+GGG | + | Chr4:23967284-23967303 | None:intergenic | 35.0% | |
| AATGGGATACCATAAAGACA+TGG | - | Chr4:23967674-23967693 | MsG0480019643.01.T01:CDS | 35.0% | |
| GCTAACACATGATGAAACAT+TGG | - | Chr4:23967543-23967562 | MsG0480019643.01.T01:CDS | 35.0% | |
| TAAGAGTTGAGAGGAATAGA+AGG | + | Chr4:23967285-23967304 | None:intergenic | 35.0% | |
| TAGAGAAAACATCGATGACA+CGG | - | Chr4:23967579-23967598 | MsG0480019643.01.T01:CDS | 35.0% | |
| ! | AGTGACTTGTAGCAATCATT+TGG | + | Chr4:23967403-23967422 | None:intergenic | 35.0% |
| ! | CTTTTTGTAGGTTGTTGAAG+TGG | + | Chr4:23967347-23967366 | None:intergenic | 35.0% |
| ! | GTGACTTGTAGCAATCATTT+GGG | + | Chr4:23967402-23967421 | None:intergenic | 35.0% |
| ! | TTCATCATGTGTTAGCTTCT+TGG | + | Chr4:23967539-23967558 | None:intergenic | 35.0% |
| !! | AGATTTTGGTGAGTGTCATT+TGG | + | Chr4:23967456-23967475 | None:intergenic | 35.0% |
| !! | TGAGTGTCATTTGGTTTGTT+TGG | + | Chr4:23967447-23967466 | None:intergenic | 35.0% |
| AACTGATGAGGGAACATGTA+CGG | - | Chr4:23967711-23967730 | MsG0480019643.01.T01:CDS | 40.0% | |
| AAGATGGTCAACACAAGTGT+TGG | - | Chr4:23967772-23967791 | MsG0480019643.01.T01:CDS | 40.0% | |
| AGCACTCATCCATGTCTTTA+TGG | + | Chr4:23967686-23967705 | None:intergenic | 40.0% | |
| GTAGCAATCATTTGGGTATG+TGG | + | Chr4:23967395-23967414 | None:intergenic | 40.0% | |
| TTCCTTGACAAGCAATGCTT+TGG | - | Chr4:23967804-23967823 | MsG0480019643.01.T01:CDS | 40.0% | |
| ! | AGTGCTGCTATAACTGATGA+GGG | - | Chr4:23967700-23967719 | MsG0480019643.01.T01:CDS | 40.0% |
| ! | TACGGATGAGTTGGATGAAA+TGG | - | Chr4:23967729-23967748 | MsG0480019643.01.T01:CDS | 40.0% |
| AGAAAACATCGATGACACGG+TGG | - | Chr4:23967582-23967601 | MsG0480019643.01.T01:CDS | 45.0% | |
| ! | GAGTGCTGCTATAACTGATG+AGG | - | Chr4:23967699-23967718 | MsG0480019643.01.T01:CDS | 45.0% |
| ! | GGTTGTTGAAGTGGACTTTG+TGG | + | Chr4:23967338-23967357 | None:intergenic | 45.0% |
| ! | TGTTGAAGTGGACTTTGTGG+TGG | + | Chr4:23967335-23967354 | None:intergenic | 45.0% |
| !! | AGCCAAAGCATTGCTTGTCA+AGG | + | Chr4:23967809-23967828 | None:intergenic | 45.0% |
| CTACTCGAGCAATCGTCTGA+CGG | - | Chr4:23967607-23967626 | MsG0480019643.01.T01:CDS | 50.0% | |
| GGGAACATGTACGGATGAGT+TGG | - | Chr4:23967720-23967739 | MsG0480019643.01.T01:CDS | 50.0% | |
| TACTCGAGCAATCGTCTGAC+GGG | - | Chr4:23967608-23967627 | MsG0480019643.01.T01:CDS | 50.0% | |
| ! | GACGGGCTCGTGCATTTGAA+TGG | - | Chr4:23967625-23967644 | MsG0480019643.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 23967241 | 23967852 | 23967241 | ID=MsG0480019643.01;Name=MsG0480019643.01 |
| Chr4 | mRNA | 23967241 | 23967852 | 23967241 | ID=MsG0480019643.01.T01;Parent=MsG0480019643.01;Name=MsG0480019643.01.T01;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|203 |
| Chr4 | exon | 23967241 | 23967852 | 23967241 | ID=MsG0480019643.01.T01:exon:24787;Parent=MsG0480019643.01.T01 |
| Chr4 | CDS | 23967241 | 23967852 | 23967241 | ID=MsG0480019643.01.T01:cds;Parent=MsG0480019643.01.T01 |
| Gene Sequence |
| Protein sequence |
