AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020691.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020691.01.T01 MTR_4g057240 94.030 201 5 2 1 195 1 200 2.99e-131 367
MsG0480020691.01.T01 MTR_7g114240 64.384 146 45 2 56 195 3 147 6.68e-59 182
MsG0480020691.01.T01 MTR_6g029200 56.164 146 58 1 56 195 9 154 1.43e-48 155
MsG0480020691.01.T01 MTR_6g029200 58.156 141 51 2 63 195 4 144 1.17e-47 153
MsG0480020691.01.T01 MTR_7g114240 56.863 102 37 2 56 151 3 103 1.65e-31 111
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020691.01.T01 AT2G28190 69.608 204 50 4 3 195 8 210 4.77e-89 260
MsG0480020691.01.T01 AT1G08830 63.014 146 47 2 56 195 3 147 1.17e-55 173
MsG0480020691.01.T01 AT1G08830 63.014 146 47 2 56 195 3 147 1.17e-55 173
MsG0480020691.01.T01 AT5G18100 56.849 146 56 2 56 195 9 153 3.69e-51 162
MsG0480020691.01.T01 AT5G18100 55.118 127 50 2 56 176 9 134 8.92e-41 135
MsG0480020691.01.T01 AT1G12520 36.441 118 67 5 57 172 28 139 1.82e-11 60.8
MsG0480020691.01.T01 AT1G12520 36.441 118 67 5 57 172 164 275 3.01e-11 61.6
MsG0480020691.01.T01 AT1G12520 36.441 118 67 5 57 172 73 184 6.03e-11 60.1

Find 63 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAACAGCAACAGCTTTCT+TGG 0.257195 4:+46303879 None:intergenic
GGCCTTACTCCAGGGCTTCA+TGG 0.278416 4:-46303006 MsG0480020691.01.T01:CDS
GGCTCTCCCAATGACTGAAT+TGG 0.294518 4:+46302345 None:intergenic
GAAAGCTGTTGCTGTTCTTA+AGG 0.320183 4:-46303875 MsG0480020691.01.T01:CDS
AACGGAGAGAGGCTTGAAAT+TGG 0.326119 4:+46303912 None:intergenic
TTTATCTTGCAGCACGAGTA+TGG 0.331157 4:-46302887 MsG0480020691.01.T01:intron
GCTCTCCCAATGACTGAATT+GGG 0.359785 4:+46302346 None:intergenic
AAAGCTGTTGCTGTTCTTAA+GGG 0.366098 4:-46303874 MsG0480020691.01.T01:CDS
GCATGACGGATTTCATCTTC+AGG 0.389426 4:+46302705 None:intergenic
CGTATCACTGGCCTTACTCC+AGG 0.406214 4:-46303015 MsG0480020691.01.T01:CDS
GGCAACAATCGTTGACAATC+AGG 0.410618 4:-46302547 MsG0480020691.01.T01:intron
TTGTTAGGTGCATGAAGATT+AGG 0.415191 4:+46304049 None:intergenic
GAGTGAGAAGTGAGTGGCGA+AGG 0.415982 4:+46304003 None:intergenic
ACTGGCCCCAATTCAGTCAT+TGG 0.417542 4:-46302352 MsG0480020691.01.T01:CDS
AATCCGTCATGCGGGTGACC+TGG 0.434387 4:-46302694 MsG0480020691.01.T01:CDS
ATACGAACATTAACTGTTGT+TGG 0.436408 4:+46303033 None:intergenic
AACCATGAAGCCCTGGAGTA+AGG 0.443025 4:+46303004 None:intergenic
AGTCATTGGGAGAGCCTTAG+TGG 0.466496 4:-46302338 MsG0480020691.01.T01:CDS
AAGGGTAACTCCACTGTTGA+AGG 0.471786 4:-46303856 MsG0480020691.01.T01:CDS
CTGGCCCCAATTCAGTCATT+GGG 0.477587 4:-46302351 MsG0480020691.01.T01:CDS
CTTGAAGATGACCTTGGAAA+GGG 0.496504 4:-46302307 MsG0480020691.01.T01:intron
CATCAGCAATTATGTTTCCC+AGG 0.498669 4:+46302676 None:intergenic
ACAGTTAATGTTCGTATCAC+TGG 0.500510 4:-46303027 MsG0480020691.01.T01:CDS
CTTCTCCGATCATCTTTCTC+CGG 0.517355 4:-46303979 MsG0480020691.01.T01:CDS
CGCGAAAGGGTCAAGAATTG+AGG 0.518782 4:+46303937 None:intergenic
GAGAGTGTGACGCCATTGTT+AGG 0.519424 4:+46304034 None:intergenic
ATCCGTCATGCGGGTGACCT+GGG 0.530041 4:-46302693 MsG0480020691.01.T01:CDS
CTTGAAATTGGAACGCGAAA+GGG 0.542665 4:+46303924 None:intergenic
GTATCACTGGCCTTACTCCA+GGG 0.550072 4:-46303014 MsG0480020691.01.T01:CDS
AGGTGAAAACCATGAAGCCC+TGG 0.553380 4:+46302997 None:intergenic
ACCAATGGGTGTATCTCGAC+AGG 0.554865 4:-46302857 MsG0480020691.01.T01:CDS
AGTTTGAGCACTGGAAATGC+CGG 0.557392 4:-46301985 MsG0480020691.01.T01:CDS
TTCAAGCTCATGAACCACTA+AGG 0.558296 4:+46302324 None:intergenic
CATGAGCTTGAAGATGACCT+TGG 0.559793 4:-46302313 MsG0480020691.01.T01:CDS
GAAGATGAAATCCGTCATGC+GGG 0.564092 4:-46302702 MsG0480020691.01.T01:CDS
CATGAACTCAGTTTGAGCAC+TGG 0.571075 4:-46301994 MsG0480020691.01.T01:CDS
GCTTGAAATTGGAACGCGAA+AGG 0.571306 4:+46303923 None:intergenic
ACCTGTCGAGATACACCCAT+TGG 0.575585 4:+46302856 None:intergenic
AGAGAGCTTGACGGAGACGC+CGG 0.577978 4:+46303960 None:intergenic
AGTAACAACACCTTCAACAG+TGG 0.579962 4:+46303846 None:intergenic
GCTTGAAGATGACCTTGGAA+AGG 0.581249 4:-46302308 MsG0480020691.01.T01:CDS
AACATAATTGCTGATGCCAA+CGG 0.589398 4:-46302669 MsG0480020691.01.T01:intron
GAGTATGGTGATACCACCAA+TGG 0.595372 4:-46302872 MsG0480020691.01.T01:CDS
GTGAAACACAGGCGTTGCAG+AGG 0.595775 4:-46302568 MsG0480020691.01.T01:intron
GGAAGAACTAACCCTTTCCA+AGG 0.597171 4:+46302296 None:intergenic
AGTATGGTGATACCACCAAT+GGG 0.608769 4:-46302871 MsG0480020691.01.T01:CDS
GAATTGAGGAGAGAGCTTGA+CGG 0.609197 4:+46303951 None:intergenic
TTTCTTGGCAGCAGCAACAA+CGG 0.616837 4:+46303894 None:intergenic
GCAGCAGCAACAACGGAGAG+AGG 0.618791 4:+46303901 None:intergenic
TGTCGAGATACACCCATTGG+TGG 0.618804 4:+46302859 None:intergenic
CTCTCCCAATGACTGAATTG+GGG 0.619637 4:+46302347 None:intergenic
ACCACAAGCTAATCTTCCAC+CGG 0.621370 4:+46301966 None:intergenic
AATCTTCATGCACCTAACAA+TGG 0.624185 4:-46304046 MsG0480020691.01.T01:exon
AATCCTAACCAGTTGACACA+CGG 0.627360 4:-46302732 MsG0480020691.01.T01:intron
TTCCCAGGTCACCCGCATGA+CGG 0.629118 4:+46302691 None:intergenic
TGAAGATGAAATCCGTCATG+CGG 0.631482 4:-46302703 MsG0480020691.01.T01:CDS
ATCATGCAGATACCACTCAC+TGG 0.633823 4:-46302370 MsG0480020691.01.T01:intron
AGACGCCGGAGAAAGATGAT+CGG 0.638557 4:+46303974 None:intergenic
TTGAGCACTGGAAATGCCGG+TGG 0.641041 4:-46301982 MsG0480020691.01.T01:CDS
AGAAGAGAGTGAGAAGTGAG+TGG 0.648773 4:+46303997 None:intergenic
ACTGAATTGGGGCCAGTGAG+TGG 0.664251 4:+46302358 None:intergenic
GCCGGTGGAAGATTAGCTTG+TGG 0.683068 4:-46301967 MsG0480020691.01.T01:CDS
ACTCTTACTCAAGAAAACGA+AGG 0.698943 4:-46303826 MsG0480020691.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAAATAAATATTTAAAA+TGG + Chr4:46303810-46303829 None:intergenic 0.0%
!! ATCAATTATCAATAATAGTT+TGG - Chr4:46302320-46302339 MsG0480020691.01.T01:CDS 15.0%
!!! AACTAAATAAACAATTTTGA+AGG + Chr4:46303135-46303154 None:intergenic 15.0%
!!! ACTGTTTTTATATAATCTAT+AGG - Chr4:46302581-46302600 MsG0480020691.01.T01:intron 15.0%
!!! TAAAAAAACACATTTTTTAG+AGG + Chr4:46303179-46303198 None:intergenic 15.0%
!!! TATAAATCTTTGTAGTTTTT+GGG - Chr4:46302426-46302445 MsG0480020691.01.T01:intron 15.0%
!!! TTATAAATCTTTGTAGTTTT+TGG - Chr4:46302425-46302444 MsG0480020691.01.T01:intron 15.0%
!! AAACTAGAATAATTGAACTT+TGG - Chr4:46302384-46302403 MsG0480020691.01.T01:intron 20.0%
!! AAGTATATGTGGTAATATAA+TGG - Chr4:46302968-46302987 MsG0480020691.01.T01:intron 20.0%
!! CAGTTATCTATTGTTTATTT+GGG + Chr4:46303892-46303911 None:intergenic 20.0%
!!! AAATAAACAATTTTGAAGGA+AGG + Chr4:46303131-46303150 None:intergenic 20.0%
!!! AATAAACAATTTTGAAGGAA+GGG + Chr4:46303130-46303149 None:intergenic 20.0%
!!! AGTTTTTTTAAGAAACTACA+TGG - Chr4:46303651-46303670 MsG0480020691.01.T01:intron 20.0%
!!! CAATAATAGTTTGGTTTTTT+GGG - Chr4:46302329-46302348 MsG0480020691.01.T01:CDS 20.0%
!!! TAACTGAAGTATTTTTACTT+TGG - Chr4:46304030-46304049 MsG0480020691.01.T01:CDS 20.0%
!!! TCAATAATAGTTTGGTTTTT+TGG - Chr4:46302328-46302347 MsG0480020691.01.T01:CDS 20.0%
!!! TTGATAATTGATAACCAAAA+TGG + Chr4:46302312-46302331 None:intergenic 20.0%
!!! TTTTTTTTTTTGTGAAACAC+AGG - Chr4:46303503-46303522 MsG0480020691.01.T01:intron 20.0%
! AAAAAATGGAAACTAAGGTT+AGG + Chr4:46303259-46303278 None:intergenic 25.0%
! ATCTACAGCATAAAATCATA+TGG + Chr4:46303835-46303854 None:intergenic 25.0%
! ATTGGATAAACAACTTGATT+GGG - Chr4:46302706-46302725 MsG0480020691.01.T01:CDS 25.0%
! ATTGTTTATTTGGGTAATAC+AGG + Chr4:46303883-46303902 None:intergenic 25.0%
! CCTATTCATTGAAAACTTTA+TGG - Chr4:46303453-46303472 MsG0480020691.01.T01:intron 25.0%
! CTAGAGAGATTATGAAAATA+AGG - Chr4:46302621-46302640 MsG0480020691.01.T01:intron 25.0%
! GCAGTTATCTATTGTTTATT+TGG + Chr4:46303893-46303912 None:intergenic 25.0%
! GTAGTTATCTTAAGTATATG+TGG - Chr4:46302957-46302976 MsG0480020691.01.T01:intron 25.0%
! TATTGGATAAACAACTTGAT+TGG - Chr4:46302705-46302724 MsG0480020691.01.T01:CDS 25.0%
! TTTAAAGAACATGTTTGGAT+TGG + Chr4:46302931-46302950 None:intergenic 25.0%
!! ACTAGTTTAAAGAACATGTT+TGG + Chr4:46302936-46302955 None:intergenic 25.0%
!! CCATAAAGTTTTCAATGAAT+AGG + Chr4:46303456-46303475 None:intergenic 25.0%
!!! ATAAACAATTTTGAAGGAAG+GGG + Chr4:46303129-46303148 None:intergenic 25.0%
!!! ATATGAGCTTGTTTTGATTT+GGG - Chr4:46302467-46302486 MsG0480020691.01.T01:intron 25.0%
!!! CTTATTTTCATAATCTCTCT+AGG + Chr4:46302623-46302642 None:intergenic 25.0%
!!! TAAGCTATTTCTATAACACA+AGG - Chr4:46302544-46302563 MsG0480020691.01.T01:intron 25.0%
AACCAAAATGGAGAATTGAA+CGG + Chr4:46302300-46302319 None:intergenic 30.0%
ATACGAACATTAACTGTTGT+TGG + Chr4:46303052-46303071 None:intergenic 30.0%
ATGTAAACAAGTATTACCGT+TGG + Chr4:46303432-46303451 None:intergenic 30.0%
TTAAGCTCTTTATCCAAACA+TGG + Chr4:46302493-46302512 None:intergenic 30.0%
! GCTTCTTGAATCTCTTATTT+AGG - Chr4:46303317-46303336 MsG0480020691.01.T01:intron 30.0%
!! TTTGTGTTTTTGTGATGAGT+AGG - Chr4:46303025-46303044 MsG0480020691.01.T01:CDS 30.0%
!!! GATATGAGCTTGTTTTGATT+TGG - Chr4:46302466-46302485 MsG0480020691.01.T01:intron 30.0%
!!! GCTTATTTTCATTAGCTCTA+CGG + Chr4:46302815-46302834 None:intergenic 30.0%
!!! GTTTTTTGGGTTAGTGAAAA+TGG - Chr4:46302342-46302361 MsG0480020691.01.T01:CDS 30.0%
!!! TTAGTTTCCATTTTTTGCCA+CGG - Chr4:46303263-46303282 MsG0480020691.01.T01:intron 30.0%
AACATAATTGCTGATGCCAA+CGG - Chr4:46303413-46303432 MsG0480020691.01.T01:intron 35.0%
AACTGGTTAGGATTGAAATG+TGG + Chr4:46303344-46303363 None:intergenic 35.0%
AATCAAACCGTGGCAAAAAA+TGG + Chr4:46303273-46303292 None:intergenic 35.0%
AATCTTCATGCACCTAACAA+TGG - Chr4:46302036-46302055 MsG0480020691.01.T01:intron 35.0%
ACAGTTAATGTTCGTATCAC+TGG - Chr4:46303055-46303074 MsG0480020691.01.T01:intron 35.0%
ACAGTTAGGTACTTTCAACA+AGG + Chr4:46303679-46303698 None:intergenic 35.0%
ACCAAAATGGAGAATTGAAC+GGG + Chr4:46302299-46302318 None:intergenic 35.0%
ACTCTTACTCAAGAAAACGA+AGG - Chr4:46302256-46302275 MsG0480020691.01.T01:intron 35.0%
AGATAACTGCTACATATCAC+AGG - Chr4:46303903-46303922 MsG0480020691.01.T01:CDS 35.0%
ATTGAACGGGATTAAGAAGA+TGG + Chr4:46302286-46302305 None:intergenic 35.0%
GATAACTGCTACATATCACA+GGG - Chr4:46303904-46303923 MsG0480020691.01.T01:CDS 35.0%
GTGGCAAAAAATGGAAACTA+AGG + Chr4:46303264-46303283 None:intergenic 35.0%
GTGTCACAAGTGTAGATTAT+TGG - Chr4:46302688-46302707 MsG0480020691.01.T01:CDS 35.0%
GTTTATTTGAGCTTGTCTAC+CGG + Chr4:46302909-46302928 None:intergenic 35.0%
TAAATCAGAATTAGACCCAC+TGG + Chr4:46303632-46303651 None:intergenic 35.0%
TTGAACGGGATTAAGAAGAT+GGG + Chr4:46302285-46302304 None:intergenic 35.0%
TTGTTAGGTGCATGAAGATT+AGG + Chr4:46302036-46302055 None:intergenic 35.0%
! AAAGCTGTTGCTGTTCTTAA+GGG - Chr4:46302208-46302227 MsG0480020691.01.T01:intron 35.0%
! AAGTACCTAACTGTGTTGTT+TGG - Chr4:46303685-46303704 MsG0480020691.01.T01:intron 35.0%
! TTTTGCCAAACAACACAGTT+AGG + Chr4:46303693-46303712 None:intergenic 35.0%
AAATCACTTGTGAGGCTGTT+TGG + Chr4:46302884-46302903 None:intergenic 40.0%
AAGAACAGCAACAGCTTTCT+TGG + Chr4:46302206-46302225 None:intergenic 40.0%
AATCACTTGTGAGGCTGTTT+GGG + Chr4:46302883-46302902 None:intergenic 40.0%
AATCCTAACCAGTTGACACA+CGG - Chr4:46303350-46303369 MsG0480020691.01.T01:intron 40.0%
AGTAACAACACCTTCAACAG+TGG + Chr4:46302239-46302258 None:intergenic 40.0%
AGTATGGTGATACCACCAAT+GGG - Chr4:46303211-46303230 MsG0480020691.01.T01:intron 40.0%
CATCAGCAATTATGTTTCCC+AGG + Chr4:46303409-46303428 None:intergenic 40.0%
CTTGAAATTGGAACGCGAAA+GGG + Chr4:46302161-46302180 None:intergenic 40.0%
CTTGAAGATGACCTTGGAAA+GGG - Chr4:46303775-46303794 MsG0480020691.01.T01:intron 40.0%
TCTTCAGTCAAATCAAACCG+TGG + Chr4:46303283-46303302 None:intergenic 40.0%
TGAAGATGAAATCCGTCATG+CGG - Chr4:46303379-46303398 MsG0480020691.01.T01:intron 40.0%
TTCAAGCTCATGAACCACTA+AGG + Chr4:46303761-46303780 None:intergenic 40.0%
TTTATCTTGCAGCACGAGTA+TGG - Chr4:46303195-46303214 MsG0480020691.01.T01:intron 40.0%
! AACCATAGTTCTTTTGCCAG+TGG - Chr4:46303613-46303632 MsG0480020691.01.T01:intron 40.0%
! ACCATAGTTCTTTTGCCAGT+GGG - Chr4:46303614-46303633 MsG0480020691.01.T01:intron 40.0%
! CAACTTGATTGGGCACTTTT+TGG - Chr4:46302716-46302735 MsG0480020691.01.T01:CDS 40.0%
! GAAAGCTGTTGCTGTTCTTA+AGG - Chr4:46302207-46302226 MsG0480020691.01.T01:intron 40.0%
! TCCCGTTCAATTCTCCATTT+TGG - Chr4:46302295-46302314 MsG0480020691.01.T01:intron 40.0%
!!! GTTTTGATTTGGGCCATGTT+TGG - Chr4:46302477-46302496 MsG0480020691.01.T01:intron 40.0%
AACGGAGAGAGGCTTGAAAT+TGG + Chr4:46302173-46302192 None:intergenic 45.0%
AAGGGTAACTCCACTGTTGA+AGG - Chr4:46302226-46302245 MsG0480020691.01.T01:intron 45.0%
ACCACAAGCTAATCTTCCAC+CGG + Chr4:46304119-46304138 None:intergenic 45.0%
ACCCACTGGCAAAAGAACTA+TGG + Chr4:46303618-46303637 None:intergenic 45.0%
ACTGCTACATATCACAGGGA+AGG - Chr4:46303908-46303927 MsG0480020691.01.T01:CDS 45.0%
AGAAGAGAGTGAGAAGTGAG+TGG + Chr4:46302088-46302107 None:intergenic 45.0%
ATCATGCAGATACCACTCAC+TGG - Chr4:46303712-46303731 MsG0480020691.01.T01:intron 45.0%
CATGAACTCAGTTTGAGCAC+TGG - Chr4:46304088-46304107 MsG0480020691.01.T01:five_prime_UTR 45.0%
CATGAGCTTGAAGATGACCT+TGG - Chr4:46303769-46303788 MsG0480020691.01.T01:intron 45.0%
CTCTCCCAATGACTGAATTG+GGG + Chr4:46303738-46303757 None:intergenic 45.0%
CTTCTCCGATCATCTTTCTC+CGG - Chr4:46302103-46302122 MsG0480020691.01.T01:intron 45.0%
GAAGATGAAATCCGTCATGC+GGG - Chr4:46303380-46303399 MsG0480020691.01.T01:intron 45.0%
GAATTGAGGAGAGAGCTTGA+CGG + Chr4:46302134-46302153 None:intergenic 45.0%
GAGTATGGTGATACCACCAA+TGG - Chr4:46303210-46303229 MsG0480020691.01.T01:intron 45.0%
GCTCTCCCAATGACTGAATT+GGG + Chr4:46303739-46303758 None:intergenic 45.0%
GCTTGAAATTGGAACGCGAA+AGG + Chr4:46302162-46302181 None:intergenic 45.0%
GCTTGAAGATGACCTTGGAA+AGG - Chr4:46303774-46303793 MsG0480020691.01.T01:intron 45.0%
GGAAGAACTAACCCTTTCCA+AGG + Chr4:46303789-46303808 None:intergenic 45.0%
GGCAACAATCGTTGACAATC+AGG - Chr4:46303535-46303554 MsG0480020691.01.T01:intron 45.0%
TACCGGCAAAATCACTTGTG+AGG + Chr4:46302892-46302911 None:intergenic 45.0%
TTTCTTGGCAGCAGCAACAA+CGG + Chr4:46302191-46302210 None:intergenic 45.0%
! AGCCTCACAAGTGATTTTGC+CGG - Chr4:46302887-46302906 MsG0480020691.01.T01:intron 45.0%
!! AGTTTGAGCACTGGAAATGC+CGG - Chr4:46304097-46304116 MsG0480020691.01.T01:five_prime_UTR 45.0%
!! GCATGACGGATTTCATCTTC+AGG + Chr4:46303380-46303399 None:intergenic 45.0%
AACCATGAAGCCCTGGAGTA+AGG + Chr4:46303081-46303100 None:intergenic 50.0%
ACCAATGGGTGTATCTCGAC+AGG - Chr4:46303225-46303244 MsG0480020691.01.T01:intron 50.0%
ACCTGTCGAGATACACCCAT+TGG + Chr4:46303229-46303248 None:intergenic 50.0%
ACTGGCCCCAATTCAGTCAT+TGG - Chr4:46303730-46303749 MsG0480020691.01.T01:intron 50.0%
AGACGCCGGAGAAAGATGAT+CGG + Chr4:46302111-46302130 None:intergenic 50.0%
AGGTGAAAACCATGAAGCCC+TGG + Chr4:46303088-46303107 None:intergenic 50.0%
AGTCATTGGGAGAGCCTTAG+TGG - Chr4:46303744-46303763 MsG0480020691.01.T01:intron 50.0%
CGCGAAAGGGTCAAGAATTG+AGG + Chr4:46302148-46302167 None:intergenic 50.0%
CTGGCCCCAATTCAGTCATT+GGG - Chr4:46303731-46303750 MsG0480020691.01.T01:intron 50.0%
GCATCTGCTCGTACTTATGC+AGG - Chr4:46304061-46304080 MsG0480020691.01.T01:exon 50.0%
GGCATGAAGTAACACTCACC+AGG + Chr4:46303108-46303127 None:intergenic 50.0%
GGCTCTCCCAATGACTGAAT+TGG + Chr4:46303740-46303759 None:intergenic 50.0%
TCTGCTCGTACTTATGCAGG+TGG - Chr4:46304064-46304083 MsG0480020691.01.T01:five_prime_UTR 50.0%
TGTCGAGATACACCCATTGG+TGG + Chr4:46303226-46303245 None:intergenic 50.0%
! AGGGCTTCATGGTTTTCACC+TGG - Chr4:46303087-46303106 MsG0480020691.01.T01:intron 50.0%
! GAGAGTGTGACGCCATTGTT+AGG + Chr4:46302051-46302070 None:intergenic 50.0%
!! GTATCACTGGCCTTACTCCA+GGG - Chr4:46303068-46303087 MsG0480020691.01.T01:intron 50.0%
ACTGAATTGGGGCCAGTGAG+TGG + Chr4:46303727-46303746 None:intergenic 55.0%
GAGTGAGAAGTGAGTGGCGA+AGG + Chr4:46302082-46302101 None:intergenic 55.0%
GCACCGTGTGTCAACTGGTT+AGG + Chr4:46303356-46303375 None:intergenic 55.0%
! GTGAAACACAGGCGTTGCAG+AGG - Chr4:46303514-46303533 MsG0480020691.01.T01:intron 55.0%
!! CGTATCACTGGCCTTACTCC+AGG - Chr4:46303067-46303086 MsG0480020691.01.T01:intron 55.0%
!! GCCGGTGGAAGATTAGCTTG+TGG - Chr4:46304115-46304134 MsG0480020691.01.T01:five_prime_UTR 55.0%
!! TTGAGCACTGGAAATGCCGG+TGG - Chr4:46304100-46304119 MsG0480020691.01.T01:five_prime_UTR 55.0%
AATCCGTCATGCGGGTGACC+TGG - Chr4:46303388-46303407 MsG0480020691.01.T01:intron 60.0%
AGAGAGCTTGACGGAGACGC+CGG + Chr4:46302125-46302144 None:intergenic 60.0%
ATCCGTCATGCGGGTGACCT+GGG - Chr4:46303389-46303408 MsG0480020691.01.T01:intron 60.0%
GCAGCAGCAACAACGGAGAG+AGG + Chr4:46302184-46302203 None:intergenic 60.0%
GGCCTTACTCCAGGGCTTCA+TGG - Chr4:46303076-46303095 MsG0480020691.01.T01:intron 60.0%
TTCCCAGGTCACCCGCATGA+CGG + Chr4:46303394-46303413 None:intergenic 60.0%
!! CAGGAGCACCGTGTGTCAAC+TGG + Chr4:46303361-46303380 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 46301960 46304144 46301960 ID=MsG0480020691.01;Name=MsG0480020691.01
Chr4 mRNA 46301960 46304144 46301960 ID=MsG0480020691.01.T01;Parent=MsG0480020691.01;Name=MsG0480020691.01.T01;_AED=0.42;_eAED=0.42;_QI=83|0.66|0.42|1|0.5|0.57|7|0|195
Chr4 exon 46301960 46302021 46301960 ID=MsG0480020691.01.T01:exon:7900;Parent=MsG0480020691.01.T01
Chr4 exon 46302308 46302383 46302308 ID=MsG0480020691.01.T01:exon:7899;Parent=MsG0480020691.01.T01
Chr4 exon 46302548 46302579 46302548 ID=MsG0480020691.01.T01:exon:7898;Parent=MsG0480020691.01.T01
Chr4 exon 46302670 46302742 46302670 ID=MsG0480020691.01.T01:exon:7897;Parent=MsG0480020691.01.T01
Chr4 exon 46302853 46302897 46302853 ID=MsG0480020691.01.T01:exon:7896;Parent=MsG0480020691.01.T01
Chr4 exon 46302996 46303060 46302996 ID=MsG0480020691.01.T01:exon:7895;Parent=MsG0480020691.01.T01
Chr4 exon 46303827 46304144 46303827 ID=MsG0480020691.01.T01:exon:7894;Parent=MsG0480020691.01.T01
Chr4 five_prime_UTR 46304062 46304144 46304062 ID=MsG0480020691.01.T01:five_prime_utr;Parent=MsG0480020691.01.T01
Chr4 CDS 46303827 46304061 46303827 ID=MsG0480020691.01.T01:cds;Parent=MsG0480020691.01.T01
Chr4 CDS 46302996 46303060 46302996 ID=MsG0480020691.01.T01:cds;Parent=MsG0480020691.01.T01
Chr4 CDS 46302853 46302897 46302853 ID=MsG0480020691.01.T01:cds;Parent=MsG0480020691.01.T01
Chr4 CDS 46302670 46302742 46302670 ID=MsG0480020691.01.T01:cds;Parent=MsG0480020691.01.T01
Chr4 CDS 46302548 46302579 46302548 ID=MsG0480020691.01.T01:cds;Parent=MsG0480020691.01.T01
Chr4 CDS 46302308 46302383 46302308 ID=MsG0480020691.01.T01:cds;Parent=MsG0480020691.01.T01
Chr4 CDS 46301960 46302021 46301960 ID=MsG0480020691.01.T01:cds;Parent=MsG0480020691.01.T01
Gene Sequence

>MsG0480020691.01.T01

ATGCACCTAACAATGGCGTCACACTCTCTCATGTCACCTTCGCCACTCACTTCTCACTCTCTTCTCCGATCATCTTTCTCCGGCGTCTCCGTCAAGCTCTCTCCTCAATTCTTGACCCTTTCGCGTTCCAATTTCAAGCCTCTCTCCGTTGTTGCTGCTGCCAAGAAAGCTGTTGCTGTTCTTAAGGGTAACTCCACTGTTGAAGGTGTTGTTACTCTTACTCAAGAAAACGAAGTAGGTCCAACAACAGTTAATGTTCGTATCACTGGCCTTACTCCAGGGCTTCATGGTTTTCACCTGCACGAGTATGGTGATACCACCAATGGGTGTATCTCGACAGGTAATTTGACACACGGTGCTCCTGAAGATGAAATCCGTCATGCGGGTGACCTGGGAAACATAATTGCTGATGCCAACGGCGTTGCAGAGGCAACAATCGTTGACAATCAGATACCACTCACTGGCCCCAATTCAGTCATTGGGAGAGCCTTAGTGGTTCATGAGCTTGAAGATGACCTTGGAAAGGGTGGACATGAACTCAGTTTGAGCACTGGAAATGCCGGTGGAAGATTAGCTTGTGGTATGTAG
Protein sequence

>MsG0480020691.01.T01

MHLTMASHSLMSPSPLTSHSLLRSSFSGVSVKLSPQFLTLSRSNFKPLSVVAAAKKAVAVLKGNSTVEGVVTLTQENEVGPTTVNVRITGLTPGLHGFHLHEYGDTTNGCISTGNLTHGAPEDEIRHAGDLGNIIADANGVAEATIVDNQIPLTGPNSVIGRALVVHELEDDLGKGGHELSLSTGNAGGRLACGM*