AlfalfaGEDB | MsG0480020807.01

M. sativa cultivar ZhongmuNo.1 / MsG0480020807.01

KEGG: -
GO: GO:0008270
PFAM: PF12906

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020807.01.T01	XP_027345347.1	57.175	439	103	8	14	372	82	515	1.21E-148	440

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020807.01.T01	A0A072UK25	92.982	228	13	1	14	241	90	314	9.05e-147	429
MsG0480020807.01.T01	A0A396I7N5	92.982	228	13	1	14	241	90	314	8.54e-146	432

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180001938.01	MsG0480020807.01	0.808002	3.835653e-50	2.168107e-47
MsG0180002623.01	MsG0480020807.01	0.835434	1.681597e-56	2.056100e-53
MsG0180004005.01	MsG0480020807.01	0.817103	3.922688e-52	2.830525e-49
MsG0180004789.01	MsG0480020807.01	0.802113	6.544888e-49	3.179470e-46
MsG0180005842.01	MsG0480020807.01	0.803016	4.262806e-49	2.118959e-46
MsG0280006375.01	MsG0480020807.01	0.802811	4.700152e-49	2.324458e-46
MsG0280008894.01	MsG0480020807.01	0.801941	7.098472e-49	3.433356e-46
MsG0280011472.01	MsG0480020807.01	0.808275	3.354515e-50	1.909956e-47
MsG0380012330.01	MsG0480020807.01	0.829539	4.863687e-55	4.992387e-52
MsG0380012361.01	MsG0480020807.01	0.810057	1.392436e-50	8.310221e-48

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020807.01.T01	MTR_4g059540	92.982	228	13	1	14	241	90	314	2.29e-150	429
MsG0480020807.01.T01	MTR_4g059540	92.982	228	13	1	14	241	90	314	7.00e-150	431
MsG0480020807.01.T01	MTR_4g059540	92.982	228	13	1	14	241	90	314	2.17e-149	432
MsG0480020807.01.T01	MTR_4g059540	95.349	86	4	0	287	372	436	521	2.84e-50	176
MsG0480020807.01.T01	MTR_2g094130	57.034	263	96	10	1	256	53	305	1.93e-80	255
MsG0480020807.01.T01	MTR_2g094130	56.098	82	35	1	292	372	416	497	1.93e-19	90.1
MsG0480020807.01.T01	MTR_2g094130	57.034	263	96	10	1	256	53	305	2.13e-80	253
MsG0480020807.01.T01	MTR_2g094130	57.034	263	96	10	1	256	53	305	2.47e-80	254
MsG0480020807.01.T01	MTR_2g094130	56.098	82	35	1	292	372	421	502	2.15e-19	89.7
MsG0480020807.01.T01	MTR_2g094130	57.034	263	96	10	1	256	53	305	2.82e-80	253

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020807.01.T01	AT5G60580	52.893	242	104	7	16	256	79	311	1.68e-68	223
MsG0480020807.01.T01	AT5G60580	52.893	242	104	7	16	256	79	311	1.68e-68	223
MsG0480020807.01.T01	AT5G60580	52.893	242	104	7	16	256	79	311	2.73e-68	223
MsG0480020807.01.T01	AT5G60580	52.893	242	104	7	16	256	79	311	2.73e-68	223
MsG0480020807.01.T01	AT3G09760	42.366	262	126	8	6	256	73	320	6.98e-44	159
MsG0480020807.01.T01	AT5G03180	46.707	167	80	5	78	241	95	255	2.35e-35	134
MsG0480020807.01.T01	AT5G03180	46.707	167	80	5	78	241	130	290	4.90e-35	134
MsG0480020807.01.T01	AT5G03180	46.707	167	80	5	78	241	130	290	5.02e-35	133
MsG0480020807.01.T01	AT5G03180	46.707	167	76	6	78	241	130	286	4.03e-32	126
MsG0480020807.01.T01	AT5G03180	46.707	167	76	6	78	241	130	286	4.44e-32	125

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Find 128 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGCGAATACACCTACT+CGG	0.581781	4:-48666096	MsG0480020807.01.T01:CDS
AAAGGCGAATACACCTACTC+GGG	0.630530	4:-48666095	MsG0480020807.01.T01:CDS
AAAGTGAGAGTGAAGGGTTG+AGG	0.541365	4:-48663304	MsG0480020807.01.T01:three_prime_UTR
AAATTCACTCTCTTGGGAGT+AGG	0.568812	4:+48666250	None:intergenic
AACCAGCAAATGTTGCAAGA+AGG	0.594657	4:+48663964	None:intergenic
AACCCTGAATCTACTCATGG+TGG	0.624757	4:-48665950	MsG0480020807.01.T01:CDS
AACTTATTGTGTATAGGGTA+GGG	0.536965	4:-48663693	MsG0480020807.01.T01:three_prime_UTR
AAGTAACTACATATTGCATT+TGG	0.459146	4:+48663234	None:intergenic
AAGTTGGAATGTAGCTGTAA+AGG	0.537209	4:-48665459	MsG0480020807.01.T01:CDS
AATCTTCTGAACACTCTTCT+TGG	0.278807	4:+48666317	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACTTATTGTGTATAGGGTA+GGG	-	Chr4:48666950-48666969	MsG0480020807.01.T01:intron	30.0%
AGAAAATAAACATGTCACTG+AGG	-	Chr4:48663134-48663153	MsG0480020807.01.T01:three_prime_UTR	30.0%
ATATCAAGATCATTGTCACT+TGG	-	Chr4:48664606-48664625	MsG0480020807.01.T01:intron	30.0%
CATAAGAAAATTCACTCTCT+TGG	+	Chr4:48664403-48664422	None:intergenic	30.0%
CTGATTCATTAGAATGAACT+GGG	+	Chr4:48666535-48666554	None:intergenic	30.0%
CTGCATAACTTATTGTGTAT+AGG	-	Chr4:48666944-48666963	MsG0480020807.01.T01:intron	30.0%
CTTCTTACTAAGGTTCTTTA+AGG	-	Chr4:48663290-48663309	MsG0480020807.01.T01:three_prime_UTR	30.0%
GATGATGTTTCTTCTTACTA+AGG	-	Chr4:48663280-48663299	MsG0480020807.01.T01:three_prime_UTR	30.0%
GATTAAAATGACGGTTTACT+CGG	-	Chr4:48664118-48664137	MsG0480020807.01.T01:intron	30.0%
TAACTTATTGTGTATAGGGT+AGG	-	Chr4:48666949-48666968	MsG0480020807.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	48663075	48667590	48663075	ID=MsG0480020807.01;Name=MsG0480020807.01
Chr4	mRNA	48663075	48667590	48663075	ID=MsG0480020807.01.T01;Parent=MsG0480020807.01;Name=MsG0480020807.01.T01;_AED=0.22;_eAED=0.24;_QI=279\|0.6\|0.66\|1\|0.8\|0.66\|6\|683\|372
Chr4	exon	48663075	48664012	48663075	ID=MsG0480020807.01.T01:exon:7390;Parent=MsG0480020807.01.T01
Chr4	exon	48665152	48665205	48665152	ID=MsG0480020807.01.T01:exon:7389;Parent=MsG0480020807.01.T01
Chr4	exon	48665320	48665582	48665320	ID=MsG0480020807.01.T01:exon:7388;Parent=MsG0480020807.01.T01
Chr4	exon	48665673	48665838	48665673	ID=MsG0480020807.01.T01:exon:7387;Parent=MsG0480020807.01.T01
Chr4	exon	48665922	48666431	48665922	ID=MsG0480020807.01.T01:exon:7386;Parent=MsG0480020807.01.T01
Chr4	exon	48667441	48667590	48667441	ID=MsG0480020807.01.T01:exon:7385;Parent=MsG0480020807.01.T01
Chr4	five_prime_UTR	48667441	48667590	48667441	ID=MsG0480020807.01.T01:five_prime_utr;Parent=MsG0480020807.01.T01
Chr4	five_prime_UTR	48666303	48666431	48666303	ID=MsG0480020807.01.T01:five_prime_utr;Parent=MsG0480020807.01.T01
Chr4	CDS	48665922	48666302	48665922	ID=MsG0480020807.01.T01:cds;Parent=MsG0480020807.01.T01
Chr4	CDS	48665673	48665838	48665673	ID=MsG0480020807.01.T01:cds;Parent=MsG0480020807.01.T01
Chr4	CDS	48665320	48665582	48665320	ID=MsG0480020807.01.T01:cds;Parent=MsG0480020807.01.T01
Chr4	CDS	48665152	48665205	48665152	ID=MsG0480020807.01.T01:cds;Parent=MsG0480020807.01.T01
Chr4	CDS	48663758	48664012	48663758	ID=MsG0480020807.01.T01:cds;Parent=MsG0480020807.01.T01
Chr4	three_prime_UTR	48663075	48663757	48663075	ID=MsG0480020807.01.T01:three_prime_utr;Parent=MsG0480020807.01.T01

Gene Sequence

>MsG0480020807.01.T01

ATGCCAATGACACCAAGTCCTACTCCAACTCCTACTCCCAAGAGAGTGAATTTTCTTATGACTTCTCGTTCGGTTGATGCTCCGACAAATAATTCTCCTGGATCTGCAACACCAAAAGGCAAATCATCTATAAGAAAGATGCTGCCAAAATTGAGTTTCAGGTATAGGACACCGGCAGATATTGAAAAGGCGAATACACCTACTCGGGAAGTTTCCTCTTCGAGCACTCGAGAAAAGCCTTTAATATCAAGATCATTGTCACTTGGTAAAATATTTACTCCTAGGATGAAAAGAACTTCATCATTGCCATTAGGAGAAATTGGACATTCAAACCCTGAATCTACTCATGGTGGAAATGGAAGTGTTGGCGGTCCTCTCAGTAAAAAAGAGACTCGGCGAAAGATAGCGCGTTCTCTTTCAATGCCTGCCAACAACAGCAAGGACAAAAGTATAAGGAGGATGGATTCGTTTTTTCGTGTTGTTCCTTCTACTCCTCGAGTAAAGGAAGGAAATGAATTGTTGAAGACGTCCCCGCCAAAGGATACTGAAATCGAAGATGATGATGATGATGATGGTGAAGATATACCTGAAGAAGAGGCGGTGTGTAGAATCTGTCTGGTTGAATTATGTGAAGGAGGCGAGACCTTCAAGTTGGAATGTAGCTGTAAAGGCGAACTTGCTTTGGCTCACAAAGAATGTGCTATTAAATGGTTTAGTATAAGGGAAACAAGACATGTGATGTCTGCAAAAAGGAGGTTACAAACCTTCCTGTCACTCTATTACGGATCCAGAGTGTTCGAAATCGGAATGTGTAACTGCACGCAGTCAGCCACATCTGGATCGTCTGATCAGACTTCAAGTTCGGTTCATGTGCAAGCTGTTCTGTCAATCCTTCTTGCAACATTTGCTGGTTTTGGTGTAGTAATGAGTGGAAGTTCTATGCTTGTTGAGTTCTTTAGATGGAGAAGGAGATGGCAAGCTTCATTAGAGCAACAACATGGCCCTCTACCGATGATACAAGCAGGACAGCAACCTCGAACTGTAACAACGCCACGTTCAGGCCAATCCAACCACAATCAATCTATAGTGCAAAATCAACAAGATTCAAATCAAAGCTGA

Protein sequence

>MsG0480020807.01.T01

MPMTPSPTPTPTPKRVNFLMTSRSVDAPTNNSPGSATPKGKSSIRKMLPKLSFRYRTPADIEKANTPTREVSSSSTREKPLISRSLSLGKIFTPRMKRTSSLPLGEIGHSNPESTHGGNGSVGGPLSKKETRRKIARSLSMPANNSKDKSIRRMDSFFRVVPSTPRVKEGNELLKTSPPKDTEIEDDDDDDGEDIPEEEAVCRICLVELCEGGETFKLECSCKGELALAHKECAIKWFSIRETRHVMSAKRRLQTFLSLYYGSRVFEIGMCNCTQSATSGSSDQTSSSVHVQAVLSILLATFAGFGVVMSGSSMLVEFFRWRRRWQASLEQQHGPLPMIQAGQQPRTVTTPRSGQSNHNQSIVQNQQDSNQS*