AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016553.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016553.01.T01 MTR_3g091820 96.939 98 2 1 30 126 128 225 8.78e-62 191
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGTCCTTGATGGATCTT+AGG 0.169048 3:-85074380 None:intergenic
GGTTAAGATCTTGTCCTTGA+TGG 0.347085 3:-85074388 None:intergenic
CTTGTCCTTGATGGATCTTA+GGG 0.390118 3:-85074379 None:intergenic
TGAAAACTGCTTCTTCATCA+AGG 0.432756 3:+85074519 MsG0380016553.01.T01:CDS
AAGCTCAATGAATTTAGATA+TGG 0.438747 3:-85074450 None:intergenic
TTGTCTTGATCAGTTGTGTA+AGG 0.463233 3:-85074473 None:intergenic
CTCTCTAACATAGAAGGTGT+TGG 0.476502 3:-85074039 None:intergenic
AATTTAGGGGAATATTGGAT+TGG 0.478716 3:+85073982 MsG0380016553.01.T01:intron
CTAGACTCAGACAAAGTCAT+AGG 0.485051 3:-85074560 None:intergenic
GAAAACTGCTTCTTCATCAA+GGG 0.488666 3:+85074520 MsG0380016553.01.T01:CDS
TAATTATCCTCAGTTGTTTG+TGG 0.490761 3:-85074422 None:intergenic
TTGTTTGTGGTGGATAAGCT+AGG 0.500159 3:-85074409 None:intergenic
TGGATTGGTTAAACAATGGA+TGG 0.507956 3:+85073997 MsG0380016553.01.T01:CDS
TTATCCTCAGTTGTTTGTGG+TGG 0.529976 3:-85074419 None:intergenic
AGACAAAACTACAAGTGCTA+TGG 0.533697 3:+85074490 MsG0380016553.01.T01:CDS
ATCTATGGACACAGCTCAGT+GGG 0.545119 3:+85073546 MsG0380016553.01.T01:exon
ATATTGGATTGGTTAAACAA+TGG 0.558827 3:+85073993 MsG0380016553.01.T01:CDS
AAAACTGCTTCTTCATCAAG+GGG 0.565168 3:+85074521 MsG0380016553.01.T01:CDS
AGTGCAATGTATAATTCAAG+TGG 0.566445 3:+85074581 MsG0380016553.01.T01:CDS
CAAAACCCTAAGATCCATCA+AGG 0.571674 3:+85074374 MsG0380016553.01.T01:CDS
GGACACAGCTCAGTGGGCAC+AGG 0.600044 3:+85073552 MsG0380016553.01.T01:CDS
AATCTATGGACACAGCTCAG+TGG 0.676107 3:+85073545 MsG0380016553.01.T01:exon
TTATCCACCACAAACAACTG+AGG 0.701982 3:+85074415 MsG0380016553.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTATATAAGAAATAATAA+AGG - Chr3:85073942-85073961 None:intergenic 10.0%
!!! ATTATTTTTGAAAAATTTAG+GGG + Chr3:85073969-85073988 MsG0380016553.01.T01:intron 10.0%
!! AGAAGAAAAAATAAATGAAA+AGG - Chr3:85073802-85073821 None:intergenic 15.0%
!! ATGATACATAAACTTTATTA+TGG - Chr3:85073663-85073682 None:intergenic 15.0%
!!! ATATTTTTTGTTATTGATGA+TGG - Chr3:85074300-85074319 None:intergenic 15.0%
!! CATAGATTTAAGAAGAAAAA+TGG - Chr3:85073533-85073552 None:intergenic 20.0%
!! TAAGAAGAAAAATGGAAAAA+AGG - Chr3:85073525-85073544 None:intergenic 20.0%
!!! AAAACTGAAATTTTTAGTTG+AGG - Chr3:85073753-85073772 None:intergenic 20.0%
!!! CATTTTTCTTCTTAAATCTA+TGG + Chr3:85073531-85073550 MsG0380016553.01.T01:exon 20.0%
!!! TTTTCTTCATGATTATAAGA+AGG - Chr3:85073607-85073626 None:intergenic 20.0%
! AAGCTCAATGAATTTAGATA+TGG - Chr3:85074453-85074472 None:intergenic 25.0%
! AATAAAGGAAACAAACAAGA+AGG - Chr3:85073927-85073946 None:intergenic 25.0%
! GAAAAATGGAAAAAAGGAAA+AGG - Chr3:85073519-85073538 None:intergenic 25.0%
! TCTAGAAACTAGAAAAAAGT+GGG + Chr3:85073463-85073482 MsG0380016553.01.T01:five_prime_UTR 25.0%
! TGAAAAATTTAGGGGAATAT+TGG + Chr3:85073977-85073996 MsG0380016553.01.T01:intron 25.0%
!! TTTTAAGCTTGGGTTAATTT+TGG + Chr3:85074619-85074638 MsG0380016553.01.T01:CDS 25.0%
!!! ACAATTCATCAACTTTTTCT+TGG - Chr3:85073859-85073878 None:intergenic 25.0%
!!! ATATTGGATTGGTTAAACAA+TGG + Chr3:85073993-85074012 MsG0380016553.01.T01:CDS 25.0%
!!! CAATTCATCAACTTTTTCTT+GGG - Chr3:85073858-85073877 None:intergenic 25.0%
!!! GTTGATTATTGTTAGTTCTA+AGG + Chr3:85073690-85073709 MsG0380016553.01.T01:intron 25.0%
!!! TTTAAAGCTTGATCTTTTTG+TGG - Chr3:85074075-85074094 None:intergenic 25.0%
!!! TTTTTTGTTATTGATGATGG+AGG - Chr3:85074297-85074316 None:intergenic 25.0%
AATTTAGGGGAATATTGGAT+TGG + Chr3:85073982-85074001 MsG0380016553.01.T01:intron 30.0%
AGTGCAATGTATAATTCAAG+TGG + Chr3:85074581-85074600 MsG0380016553.01.T01:CDS 30.0%
GAAGGAAAGAAAACAGAAAA+AGG - Chr3:85073589-85073608 None:intergenic 30.0%
TAATTATCCTCAGTTGTTTG+TGG - Chr3:85074425-85074444 None:intergenic 30.0%
! ACTTTTGCAAAACTTGTAGA+AGG + Chr3:85074166-85074185 MsG0380016553.01.T01:intron 30.0%
! CTCTAGAAACTAGAAAAAAG+TGG + Chr3:85073462-85073481 MsG0380016553.01.T01:five_prime_UTR 30.0%
! CTTTGCAAGATTTTAAGCTT+GGG + Chr3:85074609-85074628 MsG0380016553.01.T01:CDS 30.0%
! CTTTTTCTTGGGAAAAAGAA+AGG - Chr3:85073847-85073866 None:intergenic 30.0%
! TCTTTAAGAAATGTTCCTGT+AGG + Chr3:85074207-85074226 MsG0380016553.01.T01:intron 30.0%
!!! CACTTTTTTCTAGTTTCTAG+AGG - Chr3:85073464-85073483 None:intergenic 30.0%
!!! GAAATTTTTAGTTGAGGATC+AGG - Chr3:85073747-85073766 None:intergenic 30.0%
AAAACTGCTTCTTCATCAAG+GGG + Chr3:85074521-85074540 MsG0380016553.01.T01:CDS 35.0%
AGACAAAACTACAAGTGCTA+TGG + Chr3:85074490-85074509 MsG0380016553.01.T01:CDS 35.0%
AGGATCAGGAAAAGTAGTAA+AGG - Chr3:85073733-85073752 None:intergenic 35.0%
CTATGTTAGAGAGAAAAGCA+AGG + Chr3:85074049-85074068 MsG0380016553.01.T01:intron 35.0%
GAAAACTGCTTCTTCATCAA+GGG + Chr3:85074520-85074539 MsG0380016553.01.T01:CDS 35.0%
TGAAAACTGCTTCTTCATCA+AGG + Chr3:85074519-85074538 MsG0380016553.01.T01:CDS 35.0%
TTAAGAAATGTTCCTGTAGG+TGG + Chr3:85074210-85074229 MsG0380016553.01.T01:intron 35.0%
TTGTCTTGATCAGTTGTGTA+AGG - Chr3:85074476-85074495 None:intergenic 35.0%
! GCAAAACTTGTAGAAGGTAT+TGG + Chr3:85074172-85074191 MsG0380016553.01.T01:intron 35.0%
! GCTTTGCAAGATTTTAAGCT+TGG + Chr3:85074608-85074627 MsG0380016553.01.T01:CDS 35.0%
! GCTTTTCTCTCTAACATAGA+AGG - Chr3:85074048-85074067 None:intergenic 35.0%
!! ATCAAGCTTTAAACTGTCCA+AGG + Chr3:85074082-85074101 MsG0380016553.01.T01:intron 35.0%
!! TGGATTGGTTAAACAATGGA+TGG + Chr3:85073997-85074016 MsG0380016553.01.T01:CDS 35.0%
!!! CAAGTTTTGCAAAAGTACCT+TGG - Chr3:85074162-85074181 None:intergenic 35.0%
CAAAACCCTAAGATCCATCA+AGG + Chr3:85074374-85074393 MsG0380016553.01.T01:CDS 40.0%
CTAGACTCAGACAAAGTCAT+AGG - Chr3:85074563-85074582 None:intergenic 40.0%
GGTTAAGATCTTGTCCTTGA+TGG - Chr3:85074391-85074410 None:intergenic 40.0%
GGTTGTGATTGTGAGATTGA+AGG - Chr3:85074276-85074295 None:intergenic 40.0%
GTGTTAGTTGAATTGCACCT+TGG - Chr3:85074102-85074121 None:intergenic 40.0%
TCTTGGGAAAAAGAAAGGTG+TGG - Chr3:85073842-85073861 None:intergenic 40.0%
TTATCCACCACAAACAACTG+AGG + Chr3:85074415-85074434 MsG0380016553.01.T01:CDS 40.0%
TTATCCTCAGTTGTTTGTGG+TGG - Chr3:85074422-85074441 None:intergenic 40.0%
! CTCTCTAACATAGAAGGTGT+TGG - Chr3:85074042-85074061 None:intergenic 40.0%
! CTTGTCCTTGATGGATCTTA+GGG - Chr3:85074382-85074401 None:intergenic 40.0%
! GATTGAAGGTGATGATGATG+AGG - Chr3:85074262-85074281 None:intergenic 40.0%
! TCTTGTCCTTGATGGATCTT+AGG - Chr3:85074383-85074402 None:intergenic 40.0%
! TTTTGTGCTGTAGCAAGATC+AGG - Chr3:85074332-85074351 None:intergenic 40.0%
!! AAAAGTACCTTGGTTGTGAG+AGG - Chr3:85074152-85074171 None:intergenic 40.0%
!! TGTAGAAGGTATTGGACTGA+AGG + Chr3:85074180-85074199 MsG0380016553.01.T01:intron 40.0%
!! TTGTTTGTGGTGGATAAGCT+AGG - Chr3:85074412-85074431 None:intergenic 40.0%
!!! GGTTTTGAGAAGCAGATTGA+GGG - Chr3:85074361-85074380 None:intergenic 40.0%
AATCTATGGACACAGCTCAG+TGG + Chr3:85073545-85073564 MsG0380016553.01.T01:exon 45.0%
ACTATAGCCTCTCACAACCA+AGG + Chr3:85074142-85074161 MsG0380016553.01.T01:intron 45.0%
AGAAATGTTCCTGTAGGTGG+TGG + Chr3:85074213-85074232 MsG0380016553.01.T01:intron 45.0%
ATCTATGGACACAGCTCAGT+GGG + Chr3:85073546-85073565 MsG0380016553.01.T01:exon 45.0%
TTTCTTGAACCACCACCTAC+AGG - Chr3:85074225-85074244 None:intergenic 45.0%
!! AGAAGGTATTGGACTGAAGG+TGG + Chr3:85074183-85074202 MsG0380016553.01.T01:intron 45.0%
!!! GGGTTTTGAGAAGCAGATTG+AGG - Chr3:85074362-85074381 None:intergenic 45.0%
!!! TATTATTTTTGAAAAATTTA+GGG + Chr3:85073968-85073987 MsG0380016553.01.T01:intron 5.0%
!!! TTATTATTTTTGAAAAATTT+AGG + Chr3:85073967-85073986 MsG0380016553.01.T01:intron 5.0%
GGACACAGCTCAGTGGGCAC+AGG + Chr3:85073552-85073571 MsG0380016553.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 85073440 85074646 85073440 ID=MsG0380016553.01;Name=MsG0380016553.01
Chr3 mRNA 85073440 85074646 85073440 ID=MsG0380016553.01.T01;Parent=MsG0380016553.01;Name=MsG0380016553.01.T01;_AED=0.32;_eAED=0.32;_QI=110|0.5|0.33|1|0|0|3|0|129
Chr3 exon 85073440 85073573 85073440 ID=MsG0380016553.01.T01:exon:16160;Parent=MsG0380016553.01.T01
Chr3 exon 85073989 85074052 85073989 ID=MsG0380016553.01.T01:exon:16161;Parent=MsG0380016553.01.T01
Chr3 exon 85074345 85074646 85074345 ID=MsG0380016553.01.T01:exon:16162;Parent=MsG0380016553.01.T01
Chr3 five_prime_UTR 85073440 85073549 85073440 ID=MsG0380016553.01.T01:five_prime_utr;Parent=MsG0380016553.01.T01
Chr3 CDS 85073550 85073573 85073550 ID=MsG0380016553.01.T01:cds;Parent=MsG0380016553.01.T01
Chr3 CDS 85073989 85074052 85073989 ID=MsG0380016553.01.T01:cds;Parent=MsG0380016553.01.T01
Chr3 CDS 85074345 85074646 85074345 ID=MsG0380016553.01.T01:cds;Parent=MsG0380016553.01.T01
Gene Sequence

>MsG0380016553.01.T01

ATGGACACAGCTCAGTGGGCACAGGGGAATATTGGATTGGTTAAACAATGGATGGATCAACTTCAAATTCAAAACCAACACCTTCTATCACAAAATAATTTCCCTCAATCTGCTTCTCAAAACCCTAAGATCCATCAAGGACAAGATCTTAACCTAGCTTATCCACCACAAACAACTGAGGATAATTATAATACCATATCTAAATTCATTGAGCTTCCTTACACAACTGATCAAGACAAAACTACAAGTGCTATGGAGCTTCTGAAAACTGCTTCTTCATCAAGGGGTTTGAATTCTTTTATGCCTATGACTTTGTCTGAGTCTAGTGCAATGTATAATTCAAGTGGTTTTGCTTTGCAAGATTTTAAGCTTGGGTTAATTTTGGTTTAG

Protein sequence

>MsG0380016553.01.T01

MDTAQWAQGNIGLVKQWMDQLQIQNQHLLSQNNFPQSASQNPKIHQGQDLNLAYPPQTTEDNYNTISKFIELPYTTDQDKTTSAMELLKTASSSRGLNSFMPMTLSESSAMYNSSGFALQDFKLGLILV*