AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180002623.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002623.01.T01 MTR_4g097175 83.486 109 4 1 65 173 42 136 8.18e-60 182
MsG0180002623.01.T01 MTR_1g023630 83.486 109 4 1 65 173 42 136 8.18e-60 182
MsG0180002623.01.T01 MTR_8g061940 83.486 109 4 1 65 173 42 136 8.18e-60 182
MsG0180002623.01.T01 MTR_8g099430 82.569 109 5 1 65 173 42 136 3.28e-59 181
MsG0180002623.01.T01 MTR_4g097170 83.486 109 4 1 65 173 83 177 6.10e-59 182
MsG0180002623.01.T01 MTR_2g082370 81.651 109 6 1 65 173 52 146 1.97e-57 177
MsG0180002623.01.T01 MTR_7g059070 80.734 109 7 1 65 173 42 136 3.95e-57 176
MsG0180002623.01.T01 MTR_5g029820 80.734 109 7 1 65 173 42 136 3.95e-57 176
MsG0180002623.01.T01 MTR_5g029770 80.734 109 7 1 65 173 42 136 3.95e-57 176
MsG0180002623.01.T01 MTR_4g065990 80.734 109 7 1 65 173 42 136 3.95e-57 176
MsG0180002623.01.T01 MTR_4g088150 80.734 109 7 1 65 173 42 136 3.95e-57 176
MsG0180002623.01.T01 MTR_2g035230 80.734 109 7 1 65 173 42 136 3.95e-57 176
MsG0180002623.01.T01 MTR_8g092720 80.734 109 7 1 65 173 42 136 3.95e-57 176
MsG0180002623.01.T01 MTR_8g063500 80.734 109 7 1 65 173 42 136 3.95e-57 176
MsG0180002623.01.T01 MTR_8g103245 80.734 109 7 1 65 173 42 136 3.95e-57 176
MsG0180002623.01.T01 MTR_8g092820 80.734 109 7 1 65 173 42 136 3.95e-57 176
MsG0180002623.01.T01 MTR_2g082310 77.982 109 10 1 65 173 42 136 1.01e-55 172
MsG0180002623.01.T01 MTR_2g082340 77.982 109 10 1 65 173 42 136 1.01e-55 172
MsG0180002623.01.T01 MTR_7g013610 80.734 109 7 1 65 173 141 235 1.24e-55 175
MsG0180002623.01.T01 MTR_2g082400 75.229 109 12 2 65 172 41 135 2.39e-50 159
MsG0180002623.01.T01 MTR_7g013600 69.725 109 19 1 65 173 19 113 1.13e-45 146
MsG0180002623.01.T01 MTR_7g023830 53.333 135 45 2 37 171 17 133 1.55e-41 136
MsG0180002623.01.T01 MTR_7g023880 68.235 85 26 1 88 171 53 137 2.18e-36 123
MsG0180002623.01.T01 MTR_8g027840 47.170 106 41 2 66 170 34 125 3.67e-24 92.0
MsG0180002623.01.T01 MTR_1g029470 81.818 55 10 0 1 55 1 55 2.17e-23 95.1
MsG0180002623.01.T01 MTR_1g029460 89.796 49 5 0 1 49 1 49 7.21e-23 94.0
MsG0180002623.01.T01 MTR_8g105150 92.857 42 2 1 124 164 28 69 2.07e-20 80.5
MsG0180002623.01.T01 MTR_5g024530 64.516 62 22 0 104 165 93 154 3.08e-19 80.1
MsG0180002623.01.T01 MTR_8g092740 89.744 39 4 0 135 173 117 155 5.61e-19 79.3
MsG0180002623.01.T01 MTR_1g023630 51.190 84 25 2 65 148 42 109 4.88e-17 72.8
MsG0180002623.01.T01 MTR_5g089120 63.265 49 18 0 119 167 6 54 1.06e-15 68.9
MsG0180002623.01.T01 MTR_5g024630 53.030 66 29 1 88 153 50 113 4.61e-15 67.8
MsG0180002623.01.T01 MTR_5g004570 54.839 62 28 0 1 62 1 62 2.53e-14 68.9
MsG0180002623.01.T01 MTR_4g128040 58.824 51 20 1 103 152 6 56 9.20e-13 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002623.01.T01 AT5G10980 83.486 109 4 1 65 173 42 136 8.56e-60 182
MsG0180002623.01.T01 AT4G40040 83.486 109 4 1 65 173 42 136 8.56e-60 182
MsG0180002623.01.T01 AT4G40040 83.486 109 4 1 65 173 42 136 8.56e-60 182
MsG0180002623.01.T01 AT4G40040 83.486 109 4 1 65 173 42 136 8.56e-60 182
MsG0180002623.01.T01 AT4G40030 83.486 109 4 1 65 173 42 136 8.56e-60 182
MsG0180002623.01.T01 AT4G40030 83.486 109 4 1 65 173 42 136 8.56e-60 182
MsG0180002623.01.T01 AT4G40030 83.486 109 4 1 65 173 42 136 8.56e-60 182
MsG0180002623.01.T01 AT4G40030 83.486 109 4 1 65 173 70 164 3.22e-59 182
MsG0180002623.01.T01 AT1G75600 81.651 109 6 1 65 173 42 136 8.52e-59 180
MsG0180002623.01.T01 AT5G65360 80.734 109 7 1 65 173 42 136 2.85e-57 176
MsG0180002623.01.T01 AT5G10390 80.734 109 7 1 65 173 42 136 2.85e-57 176
MsG0180002623.01.T01 AT5G10400 80.734 109 7 1 65 173 42 136 2.85e-57 176
MsG0180002623.01.T01 AT3G27360 80.734 109 7 1 65 173 42 136 2.85e-57 176
MsG0180002623.01.T01 AT1G09200 80.734 109 7 1 65 173 42 136 2.85e-57 176
MsG0180002623.01.T01 AT1G13370 79.817 109 8 1 65 173 42 136 1.06e-56 175
MsG0180002623.01.T01 AT1G19890 79.817 109 8 1 65 173 43 137 2.96e-56 174
MsG0180002623.01.T01 AT5G65350 67.153 137 27 2 37 173 18 136 1.61e-55 172
MsG0180002623.01.T01 AT5G12910 67.593 108 21 1 65 172 37 130 1.21e-47 151
MsG0180002623.01.T01 AT1G01370 48.624 109 40 2 65 171 80 174 2.55e-23 91.3
MsG0180002623.01.T01 AT1G01370 48.624 109 40 2 65 171 80 174 2.55e-23 91.3
MsG0180002623.01.T01 AT1G01370 48.624 109 40 2 65 171 80 174 2.55e-23 91.3
MsG0180002623.01.T01 AT1G01370 48.624 109 40 2 65 171 80 174 2.55e-23 91.3
MsG0180002623.01.T01 AT1G76700 60.000 55 22 0 1 55 1 55 2.11e-15 73.2
MsG0180002623.01.T01 AT1G21080 58.182 55 23 0 1 55 1 55 5.35e-15 72.0
MsG0180002623.01.T01 AT1G21080 58.182 55 23 0 1 55 1 55 6.04e-15 71.6
MsG0180002623.01.T01 AT1G77020 61.818 55 21 0 1 55 1 55 1.28e-14 70.9
MsG0180002623.01.T01 AT2G21510 56.364 55 24 0 1 55 1 55 3.06e-13 66.6
MsG0180002623.01.T01 AT2G21510 56.364 55 24 0 1 55 1 55 3.52e-13 66.6
MsG0180002623.01.T01 AT2G21510 56.364 55 24 0 1 55 1 55 3.52e-13 66.6
MsG0180002623.01.T01 AT2G21510 56.364 55 24 0 1 55 1 55 3.61e-13 66.6
MsG0180002623.01.T01 AT2G21510 56.364 55 24 0 1 55 1 55 3.61e-13 66.6
MsG0180002623.01.T01 AT4G39150 54.545 55 25 0 1 55 1 55 5.00e-12 63.2
MsG0180002623.01.T01 AT4G39150 54.545 55 25 0 1 55 1 55 5.00e-12 63.2
MsG0180002623.01.T01 AT4G39150 54.545 55 25 0 1 55 1 55 5.00e-12 63.2

Find 37 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTATGGCGGATTAGTATTT+TGG 0.183704 1:-41853357 MsG0180002623.01.T01:three_prime_UTR
CCATGCAGTTCTTGCACTTC+AGG 0.275497 1:-41853529 MsG0180002623.01.T01:CDS
TACCGAAGTGCAACGGTTGC+AGG 0.285394 1:+41853903 None:intergenic
GTGCAGCATTAGGATCATTT+TGG 0.296107 1:+41853988 None:intergenic
TGAAATTTGTGTGCAGCATT+AGG 0.307048 1:+41853978 None:intergenic
CCTTCGCGGTGAACGTGCTT+AGG 0.332251 1:-41853400 MsG0180002623.01.T01:exon
TCAAAAGCTCAGTACTCTTC+TGG 0.362715 1:+41853726 None:intergenic
AGCTTTGCGAATTTGATTAT+CGG 0.381909 1:+41854147 None:intergenic
GTGCAAGAACTGCATGGCTC+TGG 0.421979 1:+41853535 None:intergenic
CAGTTTGCTCGTCGCCTTCG+CGG 0.426096 1:-41853414 MsG0180002623.01.T01:CDS
TTACCTGGTTGGATTGTTTG+AGG 0.445449 1:-41853493 MsG0180002623.01.T01:CDS
GGAAGCAGCTGAGGCTTACC+TGG 0.448380 1:-41853508 MsG0180002623.01.T01:CDS
TCACGGACAAGACGCTGAAA+TGG 0.451915 1:+41853692 None:intergenic
CGAGCAAACTGAATGTCCTT+TGG 0.459254 1:+41853426 None:intergenic
TGAAATCTTGAGCAATTTCA+CGG 0.459574 1:+41853675 None:intergenic
TGAAATTGCTCAAGATTTCA+AGG 0.466768 1:-41853672 MsG0180002623.01.T01:intron
ATAGCAGTTACTCCTACTAC+TGG 0.475822 1:-41853953 MsG0180002623.01.T01:CDS
CTTCGCGGTGAACGTGCTTA+GGG 0.476687 1:-41853399 MsG0180002623.01.T01:exon
CAGTACTCTTCTGGTACTTA+CGG 0.482178 1:+41853735 None:intergenic
TGCACTTCAGGAAGCAGCTG+AGG 0.513447 1:-41853517 MsG0180002623.01.T01:CDS
GCAACGGTTGCAGGACGATA+CGG 0.518775 1:+41853912 None:intergenic
GCAGCTGAGGCTTACCTGGT+TGG 0.521313 1:-41853504 MsG0180002623.01.T01:CDS
CGCGGTGAACGTGCTTAGGG+TGG 0.576180 1:-41853396 MsG0180002623.01.T01:exon
TCGCAAAGCTTGTTATCACA+AGG 0.578010 1:-41854134 MsG0180002623.01.T01:intron
AAGCAGAAGAAGATACTTCG+AGG 0.583997 1:+41854170 None:intergenic
GTCCTGCAACCGTTGCACTT+CGG 0.588718 1:-41853905 MsG0180002623.01.T01:intron
CCTGAAGTGCAAGAACTGCA+TGG 0.601251 1:+41853529 None:intergenic
ACGAAATTACCGAAGTGCAA+CGG 0.605114 1:+41853896 None:intergenic
CCTAAGCACGTTCACCGCGA+AGG 0.618575 1:+41853400 None:intergenic
GAACGTGCTTAGGGTGGTGA+AGG 0.623913 1:-41853390 MsG0180002623.01.T01:exon
GGTTGCAGGACGATACGGTA+AGG 0.624479 1:+41853917 None:intergenic
GTGTGCAATTCATGCTAAGA+GGG 0.629194 1:-41853460 MsG0180002623.01.T01:CDS
GAGGGTGACAATTATGCCAA+AGG 0.629580 1:-41853442 MsG0180002623.01.T01:CDS
GCAGTTACTCCTACTACTGG+AGG 0.642966 1:-41853950 MsG0180002623.01.T01:CDS
TGTGTGCAATTCATGCTAAG+AGG 0.646985 1:-41853461 MsG0180002623.01.T01:CDS
GACGCTGAAATGGAAGCTTG+CGG 0.649029 1:+41853702 None:intergenic
TGTCCTCAAACAATCCAACC+AGG 0.680035 1:+41853490 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTAAAAATTGAACATTAT+AGG + Chr1:41854107-41854126 None:intergenic 15.0%
!! ACAAATTAAATAAACCAAAG+AGG + Chr1:41853785-41853804 None:intergenic 20.0%
!!! AAGTTTGTACTAGATTTTAT+TGG - Chr1:41854166-41854185 MsG0180002623.01.T01:CDS 20.0%
!!! AGAGTTTTGAATTTGAATTT+TGG - Chr1:41853531-41853550 MsG0180002623.01.T01:CDS 20.0%
!!! AGTTTGTACTAGATTTTATT+GGG - Chr1:41854167-41854186 MsG0180002623.01.T01:CDS 20.0%
!!! TTTGATATTGTTGTTATAGA+CGG - Chr1:41853815-41853834 MsG0180002623.01.T01:intron 20.0%
!!! TTTTGGATTCATACTTTTTT+AGG - Chr1:41853548-41853567 MsG0180002623.01.T01:CDS 20.0%
!!! TTTTTAGGAATTTGAATTTG+CGG - Chr1:41853563-41853582 MsG0180002623.01.T01:intron 20.0%
! TCATACTTTCTTATTGTTAC+AGG - Chr1:41853347-41853366 MsG0180002623.01.T01:three_prime_UTR 25.0%
!! AACTCTGAAAACCTAAAAAT+AGG + Chr1:41853517-41853536 None:intergenic 25.0%
!! ACTCTGAAAACCTAAAAATA+GGG + Chr1:41853516-41853535 None:intergenic 25.0%
!!! ATTTCGTTTTCCCTATTTTT+AGG - Chr1:41853503-41853522 MsG0180002623.01.T01:CDS 25.0%
!!! TAGTATTTTGGAAGGATTTA+GGG - Chr1:41854038-41854057 MsG0180002623.01.T01:intron 25.0%
!!! TTAGTATTTTGGAAGGATTT+AGG - Chr1:41854037-41854056 MsG0180002623.01.T01:intron 25.0%
!!! TTTTAGGAATTTGAATTTGC+GGG - Chr1:41853564-41853583 MsG0180002623.01.T01:intron 25.0%
AGCTTTGCGAATTTGATTAT+CGG + Chr1:41853239-41853258 None:intergenic 30.0%
TGAAATCTTGAGCAATTTCA+CGG + Chr1:41853711-41853730 None:intergenic 30.0%
TGAAATTGCTCAAGATTTCA+AGG - Chr1:41853711-41853730 MsG0180002623.01.T01:CDS 30.0%
!!! GGATCATTTTGGTTTCTATA+AGG + Chr1:41853387-41853406 None:intergenic 30.0%
TGAAATTTGTGTGCAGCATT+AGG + Chr1:41853408-41853427 None:intergenic 35.0%
TGTGAAATCTTGTTCCTCTT+TGG - Chr1:41853768-41853787 MsG0180002623.01.T01:CDS 35.0%
! ATTTGCGGGTTATGATTGTT+AGG - Chr1:41853578-41853597 MsG0180002623.01.T01:intron 35.0%
!!! GTTATGGCGGATTAGTATTT+TGG - Chr1:41854026-41854045 MsG0180002623.01.T01:intron 35.0%
AAGCAGAAGAAGATACTTCG+AGG + Chr1:41853216-41853235 None:intergenic 40.0%
ACGAAATTACCGAAGTGCAA+CGG + Chr1:41853490-41853509 None:intergenic 40.0%
ATAGCAGTTACTCCTACTAC+TGG - Chr1:41853430-41853449 MsG0180002623.01.T01:CDS 40.0%
CAGTACTCTTCTGGTACTTA+CGG + Chr1:41853651-41853670 None:intergenic 40.0%
GTGTGCAATTCATGCTAAGA+GGG - Chr1:41853923-41853942 MsG0180002623.01.T01:CDS 40.0%
TCAAAAGCTCAGTACTCTTC+TGG + Chr1:41853660-41853679 None:intergenic 40.0%
TGTGTGCAATTCATGCTAAG+AGG - Chr1:41853922-41853941 MsG0180002623.01.T01:CDS 40.0%
TTACCTGGTTGGATTGTTTG+AGG - Chr1:41853890-41853909 MsG0180002623.01.T01:intron 40.0%
! GTGCAGCATTAGGATCATTT+TGG + Chr1:41853398-41853417 None:intergenic 40.0%
!! TCGCAAAGCTTGTTATCACA+AGG - Chr1:41853249-41853268 MsG0180002623.01.T01:three_prime_UTR 40.0%
!! TTTTTGACTCCTCCAGTAGT+AGG + Chr1:41853445-41853464 None:intergenic 40.0%
!!! TGAAGGTGCTTTTAGGGTTA+TGG - Chr1:41854010-41854029 MsG0180002623.01.T01:CDS 40.0%
!!! TGGCGGATTAGTATTTTGGA+AGG - Chr1:41854030-41854049 MsG0180002623.01.T01:intron 40.0%
CGAGCAAACTGAATGTCCTT+TGG + Chr1:41853960-41853979 None:intergenic 45.0%
GAGGGTGACAATTATGCCAA+AGG - Chr1:41853941-41853960 MsG0180002623.01.T01:CDS 45.0%
TGTCCTCAAACAATCCAACC+AGG + Chr1:41853896-41853915 None:intergenic 45.0%
!!! AGGTGCTTTTAGGGTTATGG+CGG - Chr1:41854013-41854032 MsG0180002623.01.T01:CDS 45.0%
CCATGCAGTTCTTGCACTTC+AGG - Chr1:41853854-41853873 MsG0180002623.01.T01:intron 50.0%
CCTGAAGTGCAAGAACTGCA+TGG + Chr1:41853857-41853876 None:intergenic 50.0%
GACGCTGAAATGGAAGCTTG+CGG + Chr1:41853684-41853703 None:intergenic 50.0%
GCAGTTACTCCTACTACTGG+AGG - Chr1:41853433-41853452 MsG0180002623.01.T01:CDS 50.0%
TCACGGACAAGACGCTGAAA+TGG + Chr1:41853694-41853713 None:intergenic 50.0%
!!! AGGGTGGTGAAGGTGCTTTT+AGG - Chr1:41854003-41854022 MsG0180002623.01.T01:CDS 50.0%
!!! GGGTGGTGAAGGTGCTTTTA+GGG - Chr1:41854004-41854023 MsG0180002623.01.T01:CDS 50.0%
CTTCGCGGTGAACGTGCTTA+GGG - Chr1:41853984-41854003 MsG0180002623.01.T01:CDS 55.0%
GAACGTGCTTAGGGTGGTGA+AGG - Chr1:41853993-41854012 MsG0180002623.01.T01:CDS 55.0%
GCAACGGTTGCAGGACGATA+CGG + Chr1:41853474-41853493 None:intergenic 55.0%
GGTTGCAGGACGATACGGTA+AGG + Chr1:41853469-41853488 None:intergenic 55.0%
GTCCTGCAACCGTTGCACTT+CGG - Chr1:41853478-41853497 MsG0180002623.01.T01:CDS 55.0%
GTGCAAGAACTGCATGGCTC+TGG + Chr1:41853851-41853870 None:intergenic 55.0%
TACCGAAGTGCAACGGTTGC+AGG + Chr1:41853483-41853502 None:intergenic 55.0%
TGCACTTCAGGAAGCAGCTG+AGG - Chr1:41853866-41853885 MsG0180002623.01.T01:intron 55.0%
CCTAAGCACGTTCACCGCGA+AGG + Chr1:41853986-41854005 None:intergenic 60.0%
CCTTCGCGGTGAACGTGCTT+AGG - Chr1:41853983-41854002 MsG0180002623.01.T01:CDS 60.0%
GCAGCTGAGGCTTACCTGGT+TGG - Chr1:41853879-41853898 MsG0180002623.01.T01:intron 60.0%
GGAAGCAGCTGAGGCTTACC+TGG - Chr1:41853875-41853894 MsG0180002623.01.T01:intron 60.0%
! CAGTTTGCTCGTCGCCTTCG+CGG - Chr1:41853969-41853988 MsG0180002623.01.T01:CDS 60.0%
CGCGGTGAACGTGCTTAGGG+TGG - Chr1:41853987-41854006 MsG0180002623.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 41853184 41854221 41853184 ID=MsG0180002623.01;Name=MsG0180002623.01
Chr1 mRNA 41853184 41854221 41853184 ID=MsG0180002623.01.T01;Parent=MsG0180002623.01;Name=MsG0180002623.01.T01;_AED=0.18;_eAED=0.32;_QI=0|0.33|0.25|0.75|1|0.75|4|217|173
Chr1 exon 41853184 41853571 41853184 ID=MsG0180002623.01.T01:exon:21490;Parent=MsG0180002623.01.T01
Chr1 exon 41853673 41853805 41853673 ID=MsG0180002623.01.T01:exon:21489;Parent=MsG0180002623.01.T01
Chr1 exon 41853906 41854036 41853906 ID=MsG0180002623.01.T01:exon:21488;Parent=MsG0180002623.01.T01
Chr1 exon 41854135 41854221 41854135 ID=MsG0180002623.01.T01:exon:21487;Parent=MsG0180002623.01.T01
Chr1 CDS 41854135 41854221 41854135 ID=MsG0180002623.01.T01:cds;Parent=MsG0180002623.01.T01
Chr1 CDS 41853906 41854036 41853906 ID=MsG0180002623.01.T01:cds;Parent=MsG0180002623.01.T01
Chr1 CDS 41853673 41853805 41853673 ID=MsG0180002623.01.T01:cds;Parent=MsG0180002623.01.T01
Chr1 CDS 41853401 41853571 41853401 ID=MsG0180002623.01.T01:cds;Parent=MsG0180002623.01.T01
Chr1 three_prime_UTR 41853184 41853400 41853184 ID=MsG0180002623.01.T01:three_prime_utr;Parent=MsG0180002623.01.T01
Gene Sequence

>MsG0180002623.01.T01

ATGGTGAAAGAAACAGAGTATTACGATATCCTCGAAGTATCTTCTTCTGCTTCCGATAATCAAATTCGCAAAGCTTGTTATCACAAGGCAAAGCAAGTTCATCCTTATAGAAACCAAAATGATCCTAATGCTGCACACAAATTTCAGTATAGCAGTTACTCCTACTACTGGAGGAGTCAAAAAACCTTACCGTATCGTCCTGCAACCGTTGCACTTCGGTTTTCGTTGTTGTTATTTTCGATTGATGTTATTTACAGTGTTGAGATCCGTAAGTACCAGAAGAGTACTGAGCTTTTGATCCGCAAGCTTCCATTTCAGCGTCTTGTCCGTGAAATTGCTCAAGATTTCAAGACGGATCTGAGATTCCAGAGCCATGCAGTTCTTGCACTTCAGGAAGCAGCTGAGGCTTACCTGGTTGGATTGTTTGAGGACACAAATATGTGTGCAATTCATGCTAAGAGGGTGACAATTATGCCAAAGGACATTCAGTTTGCTCGTCGCCTTCGCGGTGAACGTGCTTAG

Protein sequence

>MsG0180002623.01.T01

MVKETEYYDILEVSSSASDNQIRKACYHKAKQVHPYRNQNDPNAAHKFQYSSYSYYWRSQKTLPYRPATVALRFSLLLFSIDVIYSVEIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNMCAIHAKRVTIMPKDIQFARRLRGERA*