AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380014177.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014177.01.T01 MTR_3g052330 98.000 200 1 2 1 197 1 200 1.28e-141 393
MsG0380014177.01.T01 MTR_2g023390 53.211 109 25 1 87 195 74 156 2.79e-27 101
MsG0380014177.01.T01 MTR_4g132020 32.584 178 79 5 22 193 32 174 4.85e-21 85.9
MsG0380014177.01.T01 MTR_2g094410 43.925 107 34 3 87 193 74 154 1.41e-17 76.3
MsG0380014177.01.T01 MTR_4g059320 44.860 107 32 3 87 193 78 157 4.45e-17 75.1
MsG0380014177.01.T01 MTR_2g086660 37.879 132 54 4 64 195 152 255 3.08e-16 74.7
MsG0380014177.01.T01 MTR_2g086660 36.842 114 49 3 84 197 167 257 5.20e-15 71.6
MsG0380014177.01.T01 MTR_7g105130 62.500 48 18 0 148 195 170 217 3.45e-13 65.9
MsG0380014177.01.T01 MTR_2g018175 61.224 49 17 1 147 195 80 126 1.86e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014177.01.T01 AT4G21970 54.386 114 29 4 83 196 56 146 8.22e-31 110
MsG0380014177.01.T01 AT4G04630 40.678 177 72 5 20 195 24 168 3.26e-30 109
MsG0380014177.01.T01 AT4G26950 37.234 188 66 8 10 195 7 144 1.00e-19 81.6
MsG0380014177.01.T01 AT4G26950 37.234 188 66 8 10 195 7 144 1.00e-19 81.6
MsG0380014177.01.T01 AT3G45210 40.187 107 31 4 89 195 75 148 3.23e-17 75.1
MsG0380014177.01.T01 AT2G28400 41.176 102 38 4 87 188 76 155 4.58e-17 75.1
MsG0380014177.01.T01 AT1G29640 37.692 130 46 3 67 196 46 140 1.11e-15 70.9
MsG0380014177.01.T01 AT3G15040 59.184 49 20 0 147 195 195 243 4.56e-14 68.9
MsG0380014177.01.T01 AT2G34340 62.000 50 18 1 147 196 88 136 4.72e-13 63.9
MsG0380014177.01.T01 AT5G60680 40.000 105 40 2 89 193 80 161 1.36e-12 63.2
MsG0380014177.01.T01 AT1G11700 33.628 113 47 2 84 193 112 199 2.92e-12 63.2

Find 39 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGATTGGTCAAAGATTTA+TGG 0.145804 3:+50913386 MsG0380014177.01.T01:CDS
ACTTTGAAGAAGAAGAAATT+TGG 0.205691 3:+50913174 MsG0380014177.01.T01:CDS
TGAACTTCAGATATGTAATT+TGG 0.234956 3:-50913215 None:intergenic
CTTTGAAGAAGAAGAAATTT+GGG 0.267189 3:+50913175 MsG0380014177.01.T01:CDS
GATAGTTCCTTCTTCTCCTT+TGG 0.307953 3:-50913242 None:intergenic
AGGATAAATATAGTCTAATT+AGG 0.329079 3:+50913114 MsG0380014177.01.T01:CDS
GTTTCTAGGTATAATGAATA+TGG 0.339484 3:+50913443 MsG0380014177.01.T01:CDS
ATTCTTGCAGCTGTTGGAAT+TGG 0.357285 3:-50913272 None:intergenic
CCATAAATCTTTGACCAATC+AGG 0.358217 3:-50913386 None:intergenic
GACCAATCAGGAATGTTAAC+TGG 0.371584 3:-50913374 None:intergenic
CCACTACTAGTCCTTGGAAT+TGG 0.373701 3:-50913299 None:intergenic
AGAGTACTAAGAAGGTTTCT+AGG 0.383351 3:+50913429 MsG0380014177.01.T01:CDS
CCCTTGATCACAATGATTCA+AGG 0.409615 3:-50913688 None:intergenic
GGTGTTGGGAGGACACTTAA+AGG 0.420120 3:+50913617 MsG0380014177.01.T01:CDS
GGAATCATTCTTGCAGCTGT+TGG 0.438949 3:-50913278 None:intergenic
CACATGAAATTATTTCAAGA+AGG 0.469100 3:+50913555 MsG0380014177.01.T01:CDS
AATGAATATGGTTACTATGA+AGG 0.483633 3:+50913455 MsG0380014177.01.T01:CDS
CTACTTCCACTACTAGTCCT+TGG 0.486375 3:-50913305 None:intergenic
CACCAGTTAACATTCCTGAT+TGG 0.491163 3:+50913372 MsG0380014177.01.T01:CDS
TCATAAGCCAAAGGAGAAGA+AGG 0.492413 3:+50913235 MsG0380014177.01.T01:CDS
CTTGAAATAATTTCATGTGG+TGG 0.493248 3:-50913551 None:intergenic
ATGATGATGAGTATAGTACT+AGG 0.508127 3:+50913525 MsG0380014177.01.T01:CDS
TTAGGAAGCATAGTGAGCTA+TGG 0.542528 3:+50913132 MsG0380014177.01.T01:CDS
TTATTTCAAGAAGGCTTGCA+AGG 0.546896 3:+50913564 MsG0380014177.01.T01:CDS
ACAAAACAAGAGTACTAAGA+AGG 0.549017 3:+50913421 MsG0380014177.01.T01:CDS
CAAGAATGATTCCAATTCCA+AGG 0.572753 3:+50913288 MsG0380014177.01.T01:CDS
TGCTGATTGCTGAAAAGCCA+TGG 0.574690 3:-50913351 None:intergenic
CTTCTTGAAATAATTTCATG+TGG 0.578625 3:-50913554 None:intergenic
TTGGGAGGACACTTAAAGGT+AGG 0.583242 3:+50913621 MsG0380014177.01.T01:CDS
GTAGGGATCTTAGCAAAATG+AGG 0.584011 3:+50913639 MsG0380014177.01.T01:CDS
TAATTTATCTCATGAAACCA+TGG 0.591728 3:+50913334 MsG0380014177.01.T01:CDS
GGAAGCATAGTGAGCTATGG+AGG 0.596588 3:+50913135 MsG0380014177.01.T01:CDS
CCAATTCCAAGGACTAGTAG+TGG 0.605832 3:+50913299 MsG0380014177.01.T01:CDS
ATCTGAAGTTCATAAGCCAA+AGG 0.606512 3:+50913226 MsG0380014177.01.T01:CDS
GATGATGAAGTTGTATATCA+TGG 0.608206 3:+50913479 MsG0380014177.01.T01:CDS
TGGGAGGACACTTAAAGGTA+GGG 0.611531 3:+50913622 MsG0380014177.01.T01:CDS
TGATGATGAGTATAGTACTA+GGG 0.628457 3:+50913526 MsG0380014177.01.T01:CDS
GATGAAGTTGTATATCATGG+TGG 0.704062 3:+50913482 MsG0380014177.01.T01:CDS
AGTTGTATATCATGGTGGTG+AGG 0.715304 3:+50913487 MsG0380014177.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGGATAAATATAGTCTAATT+AGG + Chr3:50913114-50913133 MsG0380014177.01.T01:CDS 20.0%
! AATGAATATGGTTACTATGA+AGG + Chr3:50913455-50913474 MsG0380014177.01.T01:CDS 25.0%
! CACATGAAATTATTTCAAGA+AGG + Chr3:50913555-50913574 MsG0380014177.01.T01:CDS 25.0%
! CTTCTTGAAATAATTTCATG+TGG - Chr3:50913557-50913576 None:intergenic 25.0%
! TAATTTATCTCATGAAACCA+TGG + Chr3:50913334-50913353 MsG0380014177.01.T01:CDS 25.0%
! TGAACTTCAGATATGTAATT+TGG - Chr3:50913218-50913237 None:intergenic 25.0%
!! ACTTTGAAGAAGAAGAAATT+TGG + Chr3:50913174-50913193 MsG0380014177.01.T01:CDS 25.0%
!! CTTTGAAGAAGAAGAAATTT+GGG + Chr3:50913175-50913194 MsG0380014177.01.T01:CDS 25.0%
!! GTTTCTAGGTATAATGAATA+TGG + Chr3:50913443-50913462 MsG0380014177.01.T01:CDS 25.0%
!!! TCATCATTTTCTGTTTTTGA+AGG + Chr3:50913596-50913615 MsG0380014177.01.T01:CDS 25.0%
AATTCTGTCCTCATAAAAAC+TGG + Chr3:50913662-50913681 MsG0380014177.01.T01:CDS 30.0%
CTTGAAATAATTTCATGTGG+TGG - Chr3:50913554-50913573 None:intergenic 30.0%
GATGATGAAGTTGTATATCA+TGG + Chr3:50913479-50913498 MsG0380014177.01.T01:CDS 30.0%
! ATGATGATGAGTATAGTACT+AGG + Chr3:50913525-50913544 MsG0380014177.01.T01:CDS 30.0%
! TGATGATGAGTATAGTACTA+GGG + Chr3:50913526-50913545 MsG0380014177.01.T01:CDS 30.0%
!! ACAAAACAAGAGTACTAAGA+AGG + Chr3:50913421-50913440 MsG0380014177.01.T01:CDS 30.0%
!!! TTTCTGTTTTTGAAGGTGTT+GGG + Chr3:50913603-50913622 MsG0380014177.01.T01:CDS 30.0%
!!! TTTTCTGTTTTTGAAGGTGT+TGG + Chr3:50913602-50913621 MsG0380014177.01.T01:CDS 30.0%
CAAGAATGATTCCAATTCCA+AGG + Chr3:50913288-50913307 MsG0380014177.01.T01:CDS 35.0%
CCATAAATCTTTGACCAATC+AGG - Chr3:50913389-50913408 None:intergenic 35.0%
GATGAAGTTGTATATCATGG+TGG + Chr3:50913482-50913501 MsG0380014177.01.T01:CDS 35.0%
TTATTTCAAGAAGGCTTGCA+AGG + Chr3:50913564-50913583 MsG0380014177.01.T01:CDS 35.0%
! AGAGTACTAAGAAGGTTTCT+AGG + Chr3:50913429-50913448 MsG0380014177.01.T01:CDS 35.0%
! ATCTGAAGTTCATAAGCCAA+AGG + Chr3:50913226-50913245 MsG0380014177.01.T01:CDS 35.0%
! CAAGGAAACCAGTTTTTATG+AGG - Chr3:50913673-50913692 None:intergenic 35.0%
! CCTGATTGGTCAAAGATTTA+TGG + Chr3:50913386-50913405 MsG0380014177.01.T01:CDS 35.0%
ATTCTTGCAGCTGTTGGAAT+TGG - Chr3:50913275-50913294 None:intergenic 40.0%
CACCAGTTAACATTCCTGAT+TGG + Chr3:50913372-50913391 MsG0380014177.01.T01:CDS 40.0%
GACCAATCAGGAATGTTAAC+TGG - Chr3:50913377-50913396 None:intergenic 40.0%
GATAGTTCCTTCTTCTCCTT+TGG - Chr3:50913245-50913264 None:intergenic 40.0%
GTAGGGATCTTAGCAAAATG+AGG + Chr3:50913639-50913658 MsG0380014177.01.T01:CDS 40.0%
TCATAAGCCAAAGGAGAAGA+AGG + Chr3:50913235-50913254 MsG0380014177.01.T01:CDS 40.0%
! AGTTGTATATCATGGTGGTG+AGG + Chr3:50913487-50913506 MsG0380014177.01.T01:CDS 40.0%
! TTAGGAAGCATAGTGAGCTA+TGG + Chr3:50913132-50913151 MsG0380014177.01.T01:CDS 40.0%
CCACTACTAGTCCTTGGAAT+TGG - Chr3:50913302-50913321 None:intergenic 45.0%
CTACTTCCACTACTAGTCCT+TGG - Chr3:50913308-50913327 None:intergenic 45.0%
GGAATCATTCTTGCAGCTGT+TGG - Chr3:50913281-50913300 None:intergenic 45.0%
TGGGAGGACACTTAAAGGTA+GGG + Chr3:50913622-50913641 MsG0380014177.01.T01:CDS 45.0%
TTGGGAGGACACTTAAAGGT+AGG + Chr3:50913621-50913640 MsG0380014177.01.T01:CDS 45.0%
! CCAATTCCAAGGACTAGTAG+TGG + Chr3:50913299-50913318 MsG0380014177.01.T01:CDS 45.0%
! TGCTGATTGCTGAAAAGCCA+TGG - Chr3:50913354-50913373 None:intergenic 45.0%
!!! CTGTTTTTGAAGGTGTTGGG+AGG + Chr3:50913606-50913625 MsG0380014177.01.T01:CDS 45.0%
! GGAAGCATAGTGAGCTATGG+AGG + Chr3:50913135-50913154 MsG0380014177.01.T01:CDS 50.0%
! GGTGTTGGGAGGACACTTAA+AGG + Chr3:50913617-50913636 MsG0380014177.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr3 gene 50913110 50913703 50913110 ID=MsG0380014177.01;Name=MsG0380014177.01
Chr3 mRNA 50913110 50913703 50913110 ID=MsG0380014177.01.T01;Parent=MsG0380014177.01;Name=MsG0380014177.01.T01;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|197
Chr3 exon 50913110 50913703 50913110 ID=MsG0380014177.01.T01:exon:9824;Parent=MsG0380014177.01.T01
Chr3 CDS 50913110 50913703 50913110 ID=MsG0380014177.01.T01:cds;Parent=MsG0380014177.01.T01
Gene Sequence

>MsG0380014177.01.T01

ATGGAGGATAAATATAGTCTAATTAGGAAGCATAGTGAGCTATGGAGGACATTGAGAGATGAAGACTTTGAAGAAGAAGAAATTTGGGATGTTGTGAAAGAAAGACCAAATTACATATCTGAAGTTCATAAGCCAAAGGAGAAGAAGGAACTATCTTCTCTTCCAATTCCAACAGCTGCAAGAATGATTCCAATTCCAAGGACTAGTAGTGGAAGTAGTAGTGCTAATTTATCTCATGAAACCATGGCTTTTCAGCAATCAGCACCAGTTAACATTCCTGATTGGTCAAAGATTTATGGTAATAATAATAAACAAAACAAGAGTACTAAGAAGGTTTCTAGGTATAATGAATATGGTTACTATGAAGGTGATGATGAAGTTGTATATCATGGTGGTGAGGATGAAGAAGATGATGATGATGATGAGTATAGTACTAGGGTTCCACCACATGAAATTATTTCAAGAAGGCTTGCAAGGAGTCAAATATCATCATTTTCTGTTTTTGAAGGTGTTGGGAGGACACTTAAAGGTAGGGATCTTAGCAAAATGAGGAATTCTGTCCTCATAAAAACTGGTTTCCTTGAATCATTGTGA

Protein sequence

>MsG0380014177.01.T01

MEDKYSLIRKHSELWRTLRDEDFEEEEIWDVVKERPNYISEVHKPKEKKELSSLPIPTAARMIPIPRTSSGSSSANLSHETMAFQQSAPVNIPDWSKIYGNNNKQNKSTKKVSRYNEYGYYEGDDEVVYHGGEDEEDDDDDEYSTRVPPHEIISRRLARSQISSFSVFEGVGRTLKGRDLSKMRNSVLIKTGFLESL*