Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030694.01.T01 | XP_003618437.1 | 94.981 | 518 | 26 | 0 | 42 | 559 | 1 | 518 | 0 | 944 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030694.01.T01 | Q9FLD5 | 62.609 | 460 | 162 | 5 | 46 | 498 | 4 | 460 | 0 | 592 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030694.01.T01 | G7KK16 | 94.981 | 518 | 26 | 0 | 42 | 559 | 1 | 518 | 0.0 | 944 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048650.01 | MsG0680030694.01 | 0.809466 | 1.866499e-50 | 1.096678e-47 |
| MsG0180000154.01 | MsG0680030694.01 | 0.811703 | 6.129953e-51 | 3.821026e-48 |
| MsG0180000198.01 | MsG0680030694.01 | 0.835588 | 1.538069e-56 | 1.889205e-53 |
| MsG0180000653.01 | MsG0680030694.01 | 0.807299 | 5.409432e-50 | 3.002160e-47 |
| MsG0180001122.01 | MsG0680030694.01 | 0.829711 | 4.418151e-55 | 4.558408e-52 |
| MsG0180001142.01 | MsG0680030694.01 | 0.801290 | 9.653871e-49 | 4.593239e-46 |
| MsG0180004114.01 | MsG0680030694.01 | 0.823412 | 1.404578e-53 | 1.208381e-50 |
| MsG0180005278.01 | MsG0680030694.01 | 0.805375 | 1.375916e-49 | 7.267630e-47 |
| MsG0180005279.01 | MsG0680030694.01 | 0.837866 | 4.036817e-57 | 5.313421e-54 |
| MsG0180006257.01 | MsG0680030694.01 | 0.801505 | 8.725585e-49 | 4.174069e-46 |
| MsG0280011163.01 | MsG0680030694.01 | 0.805997 | 1.018876e-49 | 5.468545e-47 |
| MsG0380012249.01 | MsG0680030694.01 | 0.809679 | 1.679449e-50 | 9.924246e-48 |
| MsG0380014573.01 | MsG0680030694.01 | 0.809570 | 1.772480e-50 | 1.044383e-47 |
| MsG0480022575.01 | MsG0680030694.01 | 0.816841 | 4.491737e-52 | 3.218137e-49 |
| MsG0480023898.01 | MsG0680030694.01 | 0.808054 | 3.738985e-50 | 2.116304e-47 |
| MsG0480023900.01 | MsG0680030694.01 | 0.811770 | 5.927584e-51 | 3.701545e-48 |
| MsG0580024853.01 | MsG0680030694.01 | 0.857832 | 1.247863e-62 | 3.120806e-59 |
| MsG0580025739.01 | MsG0680030694.01 | 0.801980 | 6.968354e-49 | 3.373693e-46 |
| MsG0380015001.01 | MsG0680030694.01 | 0.823379 | 1.429830e-53 | 1.228964e-50 |
| MsG0380015005.01 | MsG0680030694.01 | 0.817554 | 3.105133e-52 | 2.268519e-49 |
| MsG0380015877.01 | MsG0680030694.01 | 0.881011 | 3.421182e-70 | 1.971329e-66 |
| MsG0380015878.01 | MsG0680030694.01 | 0.846574 | 1.993572e-59 | 3.446449e-56 |
| MsG0380016708.01 | MsG0680030694.01 | 0.810166 | 1.319465e-50 | 7.896897e-48 |
| MsG0380016909.01 | MsG0680030694.01 | 0.804129 | 2.504829e-49 | 1.281281e-46 |
| MsG0380017220.01 | MsG0680030694.01 | 0.811366 | 7.256931e-51 | 4.483453e-48 |
| MsG0480018128.01 | MsG0680030694.01 | 0.807191 | 5.702065e-50 | 3.155638e-47 |
| MsG0580027832.01 | MsG0680030694.01 | 0.805487 | 1.303549e-49 | 6.905221e-47 |
| MsG0680030629.01 | MsG0680030694.01 | 0.842755 | 2.130564e-58 | 3.262184e-55 |
| MsG0680030694.01 | MsG0680030695.01 | 0.916828 | 1.113566e-85 | 3.118055e-81 |
| MsG0680030694.01 | MsG0680030696.01 | 0.851367 | 9.292472e-61 | 1.876813e-57 |
| MsG0680030694.01 | MsG0680035460.01 | 0.814618 | 1.405656e-51 | 9.477807e-49 |
| MsG0680030694.01 | MsG0680035656.01 | 0.810302 | 1.233293e-50 | 7.407176e-48 |
| MsG0680030694.01 | MsG0680035716.01 | 0.817357 | 3.438131e-52 | 2.498315e-49 |
| MsG0680030694.01 | MsG0780037859.01 | 0.815800 | 7.677564e-52 | 5.346341e-49 |
| MsG0680030694.01 | MsG0780038386.01 | 0.828620 | 8.126597e-55 | 8.120368e-52 |
| MsG0680030694.01 | MsG0780038401.01 | 0.811000 | 8.714304e-51 | 5.332877e-48 |
| MsG0680030694.01 | MsG0780039433.01 | 0.803979 | 2.691980e-49 | 1.371785e-46 |
| MsG0680030694.01 | MsG0780040679.01 | 0.801119 | 1.046397e-48 | 4.957351e-46 |
| MsG0680030694.01 | MsG0780041485.01 | 0.846245 | 2.452384e-59 | 4.194442e-56 |
| MsG0680030694.01 | MsG0780041753.01 | 0.806079 | 9.790816e-50 | 5.265775e-47 |
| MsG0680030694.01 | MsG0780041777.01 | 0.867842 | 1.015747e-65 | 3.597086e-62 |
| MsG0680030694.01 | MsG0880045590.01 | 0.860838 | 1.562490e-63 | 4.330021e-60 |
| MsG0680030694.01 | MsG0880045706.01 | 0.803886 | 2.813967e-49 | 1.430554e-46 |
| MsG0680030694.01 | MsG0880046531.01 | 0.804554 | 2.043419e-49 | 1.056724e-46 |
| MsG0280007481.01 | MsG0680030694.01 | 0.802400 | 5.712044e-49 | 2.795444e-46 |
| MsG0280007826.01 | MsG0680030694.01 | 0.806326 | 8.686298e-50 | 4.701243e-47 |
| MsG0280008073.01 | MsG0680030694.01 | 0.803755 | 2.995878e-49 | 1.517842e-46 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030694.01.T01 | MTR_6g009520 | 94.981 | 518 | 26 | 0 | 42 | 559 | 1 | 518 | 0.0 | 944 |
| MsG0680030694.01.T01 | MTR_6g009540 | 86.822 | 516 | 68 | 0 | 44 | 559 | 1 | 516 | 0.0 | 858 |
| MsG0680030694.01.T01 | MTR_6g009720 | 72.332 | 506 | 120 | 5 | 42 | 534 | 1 | 499 | 0.0 | 709 |
| MsG0680030694.01.T01 | MTR_6g009553 | 75.442 | 452 | 92 | 3 | 66 | 502 | 1 | 448 | 0.0 | 686 |
| MsG0680030694.01.T01 | MTR_6g009770 | 70.809 | 507 | 106 | 6 | 42 | 535 | 1 | 478 | 0.0 | 674 |
| MsG0680030694.01.T01 | MTR_6g042290 | 70.149 | 469 | 119 | 4 | 53 | 520 | 21 | 469 | 0.0 | 643 |
| MsG0680030694.01.T01 | MTR_7g008040 | 67.171 | 463 | 143 | 4 | 46 | 502 | 1 | 460 | 0.0 | 642 |
| MsG0680030694.01.T01 | MTR_6g009510 | 66.667 | 504 | 92 | 5 | 52 | 554 | 20 | 448 | 0.0 | 609 |
| MsG0680030694.01.T01 | MTR_4g107370 | 59.708 | 479 | 175 | 7 | 48 | 514 | 8 | 480 | 0.0 | 584 |
| MsG0680030694.01.T01 | MTR_6g009660 | 62.620 | 519 | 123 | 10 | 44 | 551 | 1 | 459 | 0.0 | 583 |
| MsG0680030694.01.T01 | MTR_1g022250 | 58.996 | 478 | 179 | 5 | 44 | 518 | 1 | 464 | 0.0 | 575 |
| MsG0680030694.01.T01 | MTR_2g030420 | 57.660 | 470 | 182 | 5 | 48 | 513 | 8 | 464 | 0.0 | 555 |
| MsG0680030694.01.T01 | MTR_1g022245 | 52.371 | 464 | 205 | 5 | 48 | 501 | 3 | 460 | 1.40e-171 | 496 |
| MsG0680030694.01.T01 | MTR_4g128260 | 44.470 | 443 | 234 | 6 | 48 | 482 | 3 | 441 | 4.83e-127 | 382 |
| MsG0680030694.01.T01 | MTR_5g020980 | 39.059 | 489 | 256 | 8 | 49 | 525 | 13 | 471 | 3.11e-116 | 354 |
| MsG0680030694.01.T01 | MTR_2g076970 | 39.879 | 494 | 252 | 12 | 53 | 542 | 7 | 459 | 2.21e-110 | 338 |
| MsG0680030694.01.T01 | MTR_2g076710 | 40.987 | 466 | 232 | 12 | 55 | 510 | 14 | 446 | 3.13e-110 | 337 |
| MsG0680030694.01.T01 | MTR_2g061820 | 37.525 | 493 | 279 | 11 | 46 | 532 | 4 | 473 | 2.28e-107 | 332 |
| MsG0680030694.01.T01 | MTR_2g076690 | 40.249 | 482 | 234 | 13 | 55 | 523 | 16 | 456 | 1.99e-106 | 327 |
| MsG0680030694.01.T01 | MTR_2g076920 | 39.357 | 498 | 245 | 12 | 53 | 540 | 13 | 463 | 2.32e-106 | 328 |
| MsG0680030694.01.T01 | MTR_8g076920 | 38.901 | 473 | 258 | 9 | 54 | 522 | 28 | 473 | 3.93e-106 | 328 |
| MsG0680030694.01.T01 | MTR_2g076840 | 41.037 | 463 | 233 | 10 | 53 | 510 | 7 | 434 | 8.50e-105 | 332 |
| MsG0680030694.01.T01 | MTR_2g076840 | 48.592 | 284 | 114 | 6 | 221 | 502 | 450 | 703 | 2.49e-82 | 272 |
| MsG0680030694.01.T01 | MTR_2g076900 | 39.167 | 480 | 257 | 10 | 53 | 526 | 7 | 457 | 6.49e-104 | 321 |
| MsG0680030694.01.T01 | MTR_2g076990 | 42.092 | 411 | 202 | 9 | 106 | 510 | 1 | 381 | 3.63e-101 | 313 |
| MsG0680030694.01.T01 | MTR_5g020990 | 37.097 | 496 | 271 | 13 | 34 | 513 | 11 | 481 | 4.37e-95 | 299 |
| MsG0680030694.01.T01 | MTR_3g103150 | 44.702 | 302 | 138 | 4 | 203 | 503 | 4 | 277 | 8.95e-83 | 260 |
| MsG0680030694.01.T01 | MTR_3g092060 | 30.128 | 468 | 258 | 12 | 49 | 502 | 2 | 414 | 3.21e-65 | 221 |
| MsG0680030694.01.T01 | MTR_7g117950 | 33.251 | 403 | 204 | 11 | 104 | 496 | 59 | 406 | 6.47e-64 | 217 |
| MsG0680030694.01.T01 | MTR_5g021020 | 38.050 | 318 | 150 | 7 | 187 | 503 | 135 | 406 | 1.90e-57 | 198 |
| MsG0680030694.01.T01 | MTR_8g027200 | 27.455 | 448 | 247 | 13 | 81 | 522 | 35 | 410 | 1.18e-49 | 178 |
| MsG0680030694.01.T01 | MTR_1g011680 | 61.481 | 135 | 47 | 3 | 369 | 501 | 2 | 133 | 3.07e-42 | 150 |
| MsG0680030694.01.T01 | MTR_5g021000 | 52.294 | 109 | 48 | 2 | 396 | 503 | 1 | 106 | 1.40e-30 | 117 |
| MsG0680030694.01.T01 | MTR_5g020720 | 39.227 | 181 | 90 | 4 | 309 | 488 | 25 | 186 | 2.74e-27 | 109 |
| MsG0680030694.01.T01 | MTR_1g113750 | 51.852 | 108 | 50 | 2 | 429 | 535 | 16 | 122 | 3.33e-22 | 92.8 |
| MsG0680030694.01.T01 | MTR_5g021010 | 48.352 | 91 | 43 | 2 | 413 | 502 | 34 | 121 | 1.69e-17 | 79.3 |
| MsG0680030694.01.T01 | MTR_1g083330 | 32.105 | 190 | 96 | 6 | 262 | 442 | 180 | 345 | 3.17e-13 | 72.4 |
| MsG0680030694.01.T01 | MTR_4g033235 | 32.105 | 190 | 96 | 6 | 262 | 442 | 181 | 346 | 3.97e-13 | 72.0 |
| MsG0680030694.01.T01 | MTR_6g059690 | 27.302 | 315 | 183 | 13 | 229 | 521 | 177 | 467 | 3.63e-12 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030694.01.T01 | AT5G40010 | 62.609 | 460 | 162 | 5 | 46 | 498 | 4 | 460 | 0.0 | 592 |
| MsG0680030694.01.T01 | AT3G28580 | 60.606 | 462 | 163 | 8 | 46 | 498 | 4 | 455 | 0.0 | 551 |
| MsG0680030694.01.T01 | AT3G28510 | 59.002 | 461 | 187 | 2 | 47 | 507 | 5 | 463 | 0.0 | 533 |
| MsG0680030694.01.T01 | AT3G28580 | 57.143 | 462 | 157 | 9 | 46 | 498 | 4 | 433 | 2.77e-176 | 507 |
| MsG0680030694.01.T01 | AT3G28540 | 57.298 | 459 | 183 | 6 | 47 | 500 | 5 | 455 | 3.34e-172 | 498 |
| MsG0680030694.01.T01 | AT3G28540 | 57.298 | 459 | 183 | 6 | 47 | 500 | 5 | 455 | 4.69e-172 | 497 |
| MsG0680030694.01.T01 | AT3G28610 | 51.351 | 481 | 175 | 10 | 51 | 506 | 4 | 450 | 1.54e-165 | 479 |
| MsG0680030694.01.T01 | AT3G28600 | 51.357 | 479 | 190 | 8 | 42 | 506 | 1 | 450 | 1.95e-163 | 474 |
| MsG0680030694.01.T01 | AT5G40000 | 48.770 | 488 | 207 | 10 | 44 | 515 | 2 | 462 | 4.93e-156 | 455 |
| MsG0680030694.01.T01 | AT3G28520 | 53.498 | 486 | 208 | 7 | 58 | 534 | 2 | 478 | 4.41e-154 | 450 |
| MsG0680030694.01.T01 | AT3G28600 | 48.434 | 479 | 165 | 8 | 42 | 506 | 1 | 411 | 9.20e-145 | 425 |
| MsG0680030694.01.T01 | AT3G28570 | 47.009 | 468 | 197 | 8 | 51 | 505 | 14 | 443 | 6.25e-144 | 424 |
| MsG0680030694.01.T01 | AT3G28570 | 47.009 | 468 | 197 | 8 | 51 | 505 | 7 | 436 | 6.29e-144 | 423 |
| MsG0680030694.01.T01 | AT5G57480 | 39.448 | 507 | 285 | 7 | 48 | 544 | 3 | 497 | 2.04e-126 | 381 |
| MsG0680030694.01.T01 | AT4G25835 | 41.263 | 475 | 266 | 7 | 48 | 518 | 3 | 468 | 3.87e-125 | 377 |
| MsG0680030694.01.T01 | AT4G30250 | 40.336 | 476 | 262 | 8 | 44 | 507 | 1 | 466 | 1.32e-114 | 350 |
| MsG0680030694.01.T01 | AT4G30250 | 40.295 | 474 | 261 | 8 | 46 | 507 | 1 | 464 | 5.46e-114 | 349 |
| MsG0680030694.01.T01 | AT3G50940 | 39.866 | 449 | 225 | 9 | 69 | 512 | 43 | 451 | 1.17e-108 | 333 |
| MsG0680030694.01.T01 | AT3G50930 | 39.104 | 491 | 269 | 10 | 61 | 545 | 73 | 539 | 1.75e-108 | 337 |
| MsG0680030694.01.T01 | AT2G18193 | 39.419 | 482 | 257 | 10 | 54 | 532 | 19 | 468 | 4.59e-106 | 328 |
| MsG0680030694.01.T01 | AT5G17740 | 36.452 | 513 | 278 | 12 | 47 | 536 | 11 | 498 | 4.42e-102 | 318 |
| MsG0680030694.01.T01 | AT4G05380 | 38.936 | 470 | 256 | 10 | 45 | 509 | 11 | 454 | 3.02e-101 | 314 |
| MsG0680030694.01.T01 | AT5G17730 | 36.550 | 487 | 262 | 12 | 51 | 523 | 15 | 468 | 1.13e-100 | 313 |
| MsG0680030694.01.T01 | AT5G17760 | 40.000 | 445 | 213 | 9 | 109 | 528 | 72 | 487 | 1.72e-100 | 313 |
| MsG0680030694.01.T01 | AT2G18190 | 37.367 | 471 | 264 | 9 | 38 | 505 | 4 | 446 | 2.65e-99 | 310 |
| MsG0680030694.01.T01 | AT1G43910 | 38.333 | 480 | 266 | 10 | 38 | 512 | 3 | 457 | 8.06e-97 | 303 |
| MsG0680030694.01.T01 | AT5G17750 | 34.499 | 429 | 220 | 10 | 49 | 467 | 13 | 390 | 5.67e-82 | 262 |
| MsG0680030694.01.T01 | AT2G46620 | 30.081 | 492 | 293 | 8 | 46 | 532 | 1 | 446 | 2.54e-70 | 234 |
| MsG0680030694.01.T01 | AT5G17760 | 37.405 | 262 | 138 | 4 | 109 | 355 | 72 | 322 | 9.87e-52 | 181 |
| MsG0680030694.01.T01 | AT3G28560 | 42.661 | 218 | 115 | 5 | 47 | 262 | 5 | 214 | 1.39e-47 | 167 |
| MsG0680030694.01.T01 | AT3G29800 | 32.087 | 321 | 166 | 8 | 184 | 499 | 104 | 377 | 3.85e-45 | 166 |
| MsG0680030694.01.T01 | AT4G05340 | 50.000 | 96 | 42 | 1 | 358 | 453 | 4 | 93 | 1.33e-22 | 92.8 |
| MsG0680030694.01.T01 | AT1G09100 | 32.632 | 190 | 95 | 6 | 262 | 442 | 180 | 345 | 6.85e-14 | 74.3 |
| MsG0680030694.01.T01 | AT3G05530 | 32.105 | 190 | 96 | 6 | 262 | 442 | 181 | 346 | 2.48e-13 | 72.8 |
Find 73 sgRNAs with CRISPR-Local
Find 105 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAGTAAGGTAACTCTTTC+TGG | 0.086559 | 6:-7286795 | MsG0680030694.01.T01:CDS |
| AAACGTGGTTATCTACTTTA+TGG | 0.185533 | 6:-7287089 | MsG0680030694.01.T01:CDS |
| TTTGAGCATCCTGCAAGATT+TGG | 0.271233 | 6:-7287212 | MsG0680030694.01.T01:CDS |
| CAAAGTATGAAGATGGGAAT+TGG | 0.285135 | 6:-7287827 | MsG0680030694.01.T01:CDS |
| CAAATAACCCTAGTAGTAAT+TGG | 0.316335 | 6:-7287271 | MsG0680030694.01.T01:CDS |
| ATAAAGTTTCATGAATTATC+AGG | 0.317330 | 6:-7287659 | MsG0680030694.01.T01:CDS |
| AAAGTATGAAGATGGGAATT+GGG | 0.332092 | 6:-7287826 | MsG0680030694.01.T01:CDS |
| AGAAAATATCTACGAAGATT+AGG | 0.335237 | 6:+7287728 | None:intergenic |
| TGAGGAGGAAGCAAAGAAAA+AGG | 0.337182 | 6:-7286421 | MsG0680030694.01.T01:CDS |
| AAAAGGGTGTGGTATTTATA+AGG | 0.337225 | 6:+7287923 | None:intergenic |
| GCTAGGTTTCCAAATCTTGC+AGG | 0.369566 | 6:+7287203 | None:intergenic |
| TCCAATTATTGAAAAGTTGT+TGG | 0.390561 | 6:-7286565 | MsG0680030694.01.T01:CDS |
| AAAGAATATTATGCTAAAGT+TGG | 0.397678 | 6:-7287122 | MsG0680030694.01.T01:CDS |
| CCAATTATTGAAAAGTTGTT+GGG | 0.400795 | 6:-7286564 | MsG0680030694.01.T01:CDS |
| AGTTCAATGGAGTCAAAGTT+TGG | 0.406209 | 6:-7287481 | MsG0680030694.01.T01:CDS |
| AGAAGAGAAGAATGAAAGTA+AGG | 0.407800 | 6:-7286808 | MsG0680030694.01.T01:CDS |
| CTTTATGGTCCTCCAGGAAC+TGG | 0.407812 | 6:-7287074 | MsG0680030694.01.T01:CDS |
| TGAAACTTTATGTGGATGTA+TGG | 0.409252 | 6:+7287671 | None:intergenic |
| TTTGTGTTGTATGAACTAAA+AGG | 0.411570 | 6:+7287906 | None:intergenic |
| AACATTTCAAAGTATGAAGA+TGG | 0.422358 | 6:-7287834 | MsG0680030694.01.T01:CDS |
| CAACACATGTTGAATGTAAG+AGG | 0.425064 | 6:+7287343 | None:intergenic |
| CTCTTACATTCAACATGTGT+TGG | 0.428090 | 6:-7287342 | MsG0680030694.01.T01:CDS |
| AGTTTGCACTAAGATATGTT+TGG | 0.446804 | 6:+7287600 | None:intergenic |
| ATTCAACATGTGTTGGATGA+AGG | 0.454671 | 6:-7287335 | MsG0680030694.01.T01:CDS |
| GATCTTCTTAAGTTCAAGAA+AGG | 0.458025 | 6:-7287146 | MsG0680030694.01.T01:CDS |
| AGATGGGAATTGGGGAACTA+TGG | 0.460698 | 6:-7287817 | MsG0680030694.01.T01:CDS |
| TCTTGAGTTAACAACAGTTA+AGG | 0.462521 | 6:-7286997 | MsG0680030694.01.T01:CDS |
| ATTGACTCAAGAGGAAGAAA+AGG | 0.464359 | 6:-7286352 | MsG0680030694.01.T01:CDS |
| ATGCTAAAGTTGGAAAGGCT+TGG | 0.473908 | 6:-7287112 | MsG0680030694.01.T01:CDS |
| AAGTCTTCAGTGATTGACTT+TGG | 0.475085 | 6:+7286498 | None:intergenic |
| AACTTGATCCTGCTCTCATA+AGG | 0.476557 | 6:-7286692 | MsG0680030694.01.T01:CDS |
| GAGGAAAAGGTGAAGGAGAA+TGG | 0.482194 | 6:-7286309 | MsG0680030694.01.T01:CDS |
| CTTCCCCTCCTTATGAGAGC+AGG | 0.493116 | 6:+7286684 | None:intergenic |
| AAGCTATTATGAGCAAGAAT+AGG | 0.495148 | 6:-7287310 | MsG0680030694.01.T01:CDS |
| CTCTCGATCTCACCGGGCAA+AGG | 0.502496 | 6:-7286899 | MsG0680030694.01.T01:CDS |
| TATCTACTTTATGGTCCTCC+AGG | 0.505261 | 6:-7287080 | MsG0680030694.01.T01:CDS |
| GAAAGAAGAATTGACTCAAG+AGG | 0.510872 | 6:-7286361 | MsG0680030694.01.T01:CDS |
| TTGTGTTGTATGAACTAAAA+GGG | 0.511187 | 6:+7287907 | None:intergenic |
| GACTTTGATTCAATCTCTTG+AGG | 0.516280 | 6:-7286460 | MsG0680030694.01.T01:CDS |
| ATAATTCATGAAACTTTATG+TGG | 0.520872 | 6:+7287663 | None:intergenic |
| AAATTCTCAGCAACATCAGC+AGG | 0.526229 | 6:+7286525 | None:intergenic |
| GAAATCATAGATGAGTTCAA+TGG | 0.530945 | 6:-7287494 | MsG0680030694.01.T01:CDS |
| GAGAATGGTGTCAAAGAGAA+TGG | 0.531190 | 6:-7286294 | MsG0680030694.01.T01:CDS |
| TCTCATAAGGAGGGGAAGAA+TGG | 0.535990 | 6:-7286679 | MsG0680030694.01.T01:CDS |
| TAACCCTAGTAGTAATTGGT+GGG | 0.547641 | 6:-7287267 | MsG0680030694.01.T01:CDS |
| AAATATCTACGAAGATTAGG+TGG | 0.547924 | 6:+7287731 | None:intergenic |
| GATGGAATTTGGTCAGCTTG+TGG | 0.549571 | 6:-7286756 | MsG0680030694.01.T01:CDS |
| AAAATTAGAGGAAAAGGTGA+AGG | 0.554515 | 6:-7286316 | MsG0680030694.01.T01:CDS |
| AAAACCACTTGTTCTTAGCA+TGG | 0.557204 | 6:-7287528 | MsG0680030694.01.T01:CDS |
| AACCCTAGTAGTAATTGGTG+GGG | 0.572012 | 6:-7287266 | MsG0680030694.01.T01:CDS |
| TCATCCATGCTAAGAACAAG+TGG | 0.573558 | 6:+7287524 | None:intergenic |
| ATATTATGCTAAAGTTGGAA+AGG | 0.575311 | 6:-7287117 | MsG0680030694.01.T01:CDS |
| GATTGTTCTCTCGATCTCAC+CGG | 0.575538 | 6:-7286906 | MsG0680030694.01.T01:CDS |
| ATTGTTCTCTCGATCTCACC+GGG | 0.579324 | 6:-7286905 | MsG0680030694.01.T01:CDS |
| TGATGAGACTGATGAAAAGA+AGG | 0.583033 | 6:-7286853 | MsG0680030694.01.T01:CDS |
| AAGTATGAAGATGGGAATTG+GGG | 0.585836 | 6:-7287825 | MsG0680030694.01.T01:CDS |
| TTGATCCTGCTCTCATAAGG+AGG | 0.589263 | 6:-7286689 | MsG0680030694.01.T01:CDS |
| TGATCCTGCTCTCATAAGGA+GGG | 0.594215 | 6:-7286688 | MsG0680030694.01.T01:CDS |
| TCAATGGAGTCAAAGTTTGG+TGG | 0.596166 | 6:-7287478 | MsG0680030694.01.T01:CDS |
| ACATTTCAAAGTATGAAGAT+GGG | 0.598757 | 6:-7287833 | MsG0680030694.01.T01:CDS |
| GTTGGAAAGGCTTGGAAACG+TGG | 0.601459 | 6:-7287104 | MsG0680030694.01.T01:CDS |
| AAGATTTGGAAACCTAGCTA+TGG | 0.607202 | 6:-7287198 | MsG0680030694.01.T01:CDS |
| GGATGAGGAAGCAAAGCTCA+AGG | 0.616773 | 6:-7286394 | MsG0680030694.01.T01:CDS |
| GAGAATGGTGTGAAAGAGAA+CGG | 0.617159 | 6:-7286279 | MsG0680030694.01.T01:CDS |
| ACCCTAGTAGTAATTGGTGG+GGG | 0.617964 | 6:-7287265 | MsG0680030694.01.T01:CDS |
| GGGGAACTATGGTCACAACT+AGG | 0.620126 | 6:-7287806 | MsG0680030694.01.T01:CDS |
| ATAACCCTAGTAGTAATTGG+TGG | 0.620183 | 6:-7287268 | MsG0680030694.01.T01:CDS |
| TTGTATGAACTAAAAGGGTG+TGG | 0.627142 | 6:+7287912 | None:intergenic |
| AATATCTACGAAGATTAGGT+GGG | 0.627753 | 6:+7287732 | None:intergenic |
| GATCCTGCTCTCATAAGGAG+GGG | 0.632718 | 6:-7286687 | MsG0680030694.01.T01:CDS |
| GTCACAACTAGGTTCAATCA+CGG | 0.654145 | 6:-7287795 | MsG0680030694.01.T01:CDS |
| TCAAGAGGAAGAAAAGGTGA+AGG | 0.669369 | 6:-7286346 | MsG0680030694.01.T01:CDS |
| GGTCAGCTTGTGGATCAGAG+AGG | 0.714357 | 6:-7286746 | MsG0680030694.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAATATTATGCTAAAGT+TGG | - | Chr6:7287088-7287107 | MsG0680030694.01.T01:CDS | 20.0% |
| !! | ATAAAGTTTCATGAATTATC+AGG | - | Chr6:7286551-7286570 | MsG0680030694.01.T01:CDS | 20.0% |
| !! | ATAATTCATGAAACTTTATG+TGG | + | Chr6:7286550-7286569 | None:intergenic | 20.0% |
| !!! | AAAATACTTTGAAGTGTTTT+TGG | + | Chr6:7286344-7286363 | None:intergenic | 20.0% |
| !!! | AATTTTCACAACAAATTTTG+TGG | - | Chr6:7287492-7287511 | MsG0680030694.01.T01:CDS | 20.0% |
| !!! | TGAATTTTATTGATGGAATT+TGG | - | Chr6:7287443-7287462 | MsG0680030694.01.T01:CDS | 20.0% |
| !!! | TTCATACTTTGAAATGTTTT+TGG | + | Chr6:7286374-7286393 | None:intergenic | 20.0% |
| ! | AACATTTCAAAGTATGAAGA+TGG | - | Chr6:7286376-7286395 | MsG0680030694.01.T01:CDS | 25.0% |
| ! | ACATTTCAAAGTATGAAGAT+GGG | - | Chr6:7286377-7286396 | MsG0680030694.01.T01:CDS | 25.0% |
| ! | AGAAAATATCTACGAAGATT+AGG | + | Chr6:7286485-7286504 | None:intergenic | 25.0% |
| ! | ATATTATGCTAAAGTTGGAA+AGG | - | Chr6:7287093-7287112 | MsG0680030694.01.T01:CDS | 25.0% |
| ! | CCAATTATTGAAAAGTTGTT+GGG | - | Chr6:7287646-7287665 | MsG0680030694.01.T01:CDS | 25.0% |
| ! | TCCAATTATTGAAAAGTTGT+TGG | - | Chr6:7287645-7287664 | MsG0680030694.01.T01:CDS | 25.0% |
| ! | TCTTGAGGAAAAAAAGAAAA+AGG | - | Chr6:7287765-7287784 | MsG0680030694.01.T01:CDS | 25.0% |
| ! | TGATGAAAAATTAGAGGAAA+AGG | - | Chr6:7287888-7287907 | MsG0680030694.01.T01:CDS | 25.0% |
| ! | TTGTGTTGTATGAACTAAAA+GGG | + | Chr6:7286306-7286325 | None:intergenic | 25.0% |
| ! | TTTGTGTTGTATGAACTAAA+AGG | + | Chr6:7286307-7286326 | None:intergenic | 25.0% |
| !! | AGAAAATGATTGTGATTTTG+AGG | + | Chr6:7286774-7286793 | None:intergenic | 25.0% |
| !!! | AAAATCTCTTGTTTCTTTTC+TGG | + | Chr6:7287040-7287059 | None:intergenic | 25.0% |
| !!! | AATCTTTTTTCATCTGAAGT+AGG | + | Chr6:7286803-7286822 | None:intergenic | 25.0% |
| !!! | GGTTTGTTGAATTTTATTGA+TGG | - | Chr6:7287436-7287455 | MsG0680030694.01.T01:CDS | 25.0% |
| !!! | TCTTTTTCAGCTTTCTTAAT+AGG | + | Chr6:7287385-7287404 | None:intergenic | 25.0% |
| AAAAAAGAAAAAGGATGAGG+AGG | - | Chr6:7287774-7287793 | MsG0680030694.01.T01:CDS | 30.0% | |
| AAAATTAGAGGAAAAGGTGA+AGG | - | Chr6:7287894-7287913 | MsG0680030694.01.T01:CDS | 30.0% | |
| AAACGTGGTTATCTACTTTA+TGG | - | Chr6:7287121-7287140 | MsG0680030694.01.T01:CDS | 30.0% | |
| AAAGTATGAAGATGGGAATT+GGG | - | Chr6:7286384-7286403 | MsG0680030694.01.T01:CDS | 30.0% | |
| AAATATCTACGAAGATTAGG+TGG | + | Chr6:7286482-7286501 | None:intergenic | 30.0% | |
| AAGCTATTATGAGCAAGAAT+AGG | - | Chr6:7286900-7286919 | MsG0680030694.01.T01:CDS | 30.0% | |
| AATATCTACGAAGATTAGGT+GGG | + | Chr6:7286481-7286500 | None:intergenic | 30.0% | |
| AGAAGAGAAGAATGAAAGTA+AGG | - | Chr6:7287402-7287421 | MsG0680030694.01.T01:CDS | 30.0% | |
| AGTTTGCACTAAGATATGTT+TGG | + | Chr6:7286613-7286632 | None:intergenic | 30.0% | |
| CAAATAACCCTAGTAGTAAT+TGG | - | Chr6:7286939-7286958 | MsG0680030694.01.T01:CDS | 30.0% | |
| GAAATCATAGATGAGTTCAA+TGG | - | Chr6:7286716-7286735 | MsG0680030694.01.T01:CDS | 30.0% | |
| GATCTTCTTAAGTTCAAGAA+AGG | - | Chr6:7287064-7287083 | MsG0680030694.01.T01:CDS | 30.0% | |
| GGAAAAAAAGAAAAAGGATG+AGG | - | Chr6:7287771-7287790 | MsG0680030694.01.T01:CDS | 30.0% | |
| TCTTGAGTTAACAACAGTTA+AGG | - | Chr6:7287213-7287232 | MsG0680030694.01.T01:CDS | 30.0% | |
| TGAAACTTTATGTGGATGTA+TGG | + | Chr6:7286542-7286561 | None:intergenic | 30.0% | |
| TGGGAAAAATTTGTCAAACA+TGG | + | Chr6:7286462-7286481 | None:intergenic | 30.0% | |
| ! | AAAAGGGTGTGGTATTTATA+AGG | + | Chr6:7286290-7286309 | None:intergenic | 30.0% |
| ! | CCCAACAACTTTTCAATAAT+TGG | + | Chr6:7287649-7287668 | None:intergenic | 30.0% |
| ! | TTGTAGCTATCAAGCTTTTA+AGG | - | Chr6:7287576-7287595 | MsG0680030694.01.T01:CDS | 30.0% |
| !! | AAATGATTGTGATTTTGAGG+TGG | + | Chr6:7286771-7286790 | None:intergenic | 30.0% |
| !! | ATGCTTTTTATGGAAGGTTA+GGG | + | Chr6:7286831-7286850 | None:intergenic | 30.0% |
| !! | GAAGGTTGATGAAAAATTAG+AGG | - | Chr6:7287882-7287901 | MsG0680030694.01.T01:CDS | 30.0% |
| AAAACCACTTGTTCTTAGCA+TGG | - | Chr6:7286682-7286701 | MsG0680030694.01.T01:CDS | 35.0% | |
| AAAGAAAAAGGTAGAGGATG+AGG | - | Chr6:7287801-7287820 | MsG0680030694.01.T01:CDS | 35.0% | |
| AAGATTTGGAAACCTAGCTA+TGG | - | Chr6:7287012-7287031 | MsG0680030694.01.T01:CDS | 35.0% | |
| AAGTATGAAGATGGGAATTG+GGG | - | Chr6:7286385-7286404 | MsG0680030694.01.T01:CDS | 35.0% | |
| AGTTCAATGGAGTCAAAGTT+TGG | - | Chr6:7286729-7286748 | MsG0680030694.01.T01:CDS | 35.0% | |
| ATAACCCTAGTAGTAATTGG+TGG | - | Chr6:7286942-7286961 | MsG0680030694.01.T01:CDS | 35.0% | |
| ATTCAACATGTGTTGGATGA+AGG | - | Chr6:7286875-7286894 | MsG0680030694.01.T01:CDS | 35.0% | |
| CAAAGTATGAAGATGGGAAT+TGG | - | Chr6:7286383-7286402 | MsG0680030694.01.T01:CDS | 35.0% | |
| CAACACATGTTGAATGTAAG+AGG | + | Chr6:7286870-7286889 | None:intergenic | 35.0% | |
| CTCTTACATTCAACATGTGT+TGG | - | Chr6:7286868-7286887 | MsG0680030694.01.T01:CDS | 35.0% | |
| GAAAGTAAGGTAACTCTTTC+TGG | - | Chr6:7287415-7287434 | MsG0680030694.01.T01:CDS | 35.0% | |
| GACTTTGATTCAATCTCTTG+AGG | - | Chr6:7287750-7287769 | MsG0680030694.01.T01:CDS | 35.0% | |
| TAACCCTAGTAGTAATTGGT+GGG | - | Chr6:7286943-7286962 | MsG0680030694.01.T01:CDS | 35.0% | |
| TGATGAGACTGATGAAAAGA+AGG | - | Chr6:7287357-7287376 | MsG0680030694.01.T01:CDS | 35.0% | |
| TTGTATGAACTAAAAGGGTG+TGG | + | Chr6:7286301-7286320 | None:intergenic | 35.0% | |
| ! | AAGTCTTCAGTGATTGACTT+TGG | + | Chr6:7287715-7287734 | None:intergenic | 35.0% |
| ! | ATTGACTCAAGAGGAAGAAA+AGG | - | Chr6:7287858-7287877 | MsG0680030694.01.T01:CDS | 35.0% |
| ! | ATTGTAGATTTTCCAGTTCC+TGG | + | Chr6:7287151-7287170 | None:intergenic | 35.0% |
| ! | GAAAGAAGAATTGACTCAAG+AGG | - | Chr6:7287849-7287868 | MsG0680030694.01.T01:CDS | 35.0% |
| ! | TGCTAAGAACAAGTGGTTTT+TGG | + | Chr6:7286682-7286701 | None:intergenic | 35.0% |
| !! | GATGCTTTTTATGGAAGGTT+AGG | + | Chr6:7286832-7286851 | None:intergenic | 35.0% |
| !! | TGAGTTCACGATGCTTTTTA+TGG | + | Chr6:7286841-7286860 | None:intergenic | 35.0% |
| !! | TTCACGATGCTTTTTATGGA+AGG | + | Chr6:7286837-7286856 | None:intergenic | 35.0% |
| !!! | TTTTTTCTTCTTCCTTTGCC+CGG | + | Chr6:7287326-7287345 | None:intergenic | 35.0% |
| AAATTCTCAGCAACATCAGC+AGG | + | Chr6:7287688-7287707 | None:intergenic | 40.0% | |
| AACCCTAGTAGTAATTGGTG+GGG | - | Chr6:7286944-7286963 | MsG0680030694.01.T01:CDS | 40.0% | |
| AACTTGATCCTGCTCTCATA+AGG | - | Chr6:7287518-7287537 | MsG0680030694.01.T01:CDS | 40.0% | |
| ATGCTAAAGTTGGAAAGGCT+TGG | - | Chr6:7287098-7287117 | MsG0680030694.01.T01:CDS | 40.0% | |
| GAGAATGGTGTGAAAGAGAA+CGG | - | Chr6:7287931-7287950 | MsG0680030694.01.T01:CDS | 40.0% | |
| GGAAGCAAAGAAAAAGGTAG+AGG | - | Chr6:7287795-7287814 | MsG0680030694.01.T01:CDS | 40.0% | |
| GTCACAACTAGGTTCAATCA+CGG | - | Chr6:7286415-7286434 | MsG0680030694.01.T01:CDS | 40.0% | |
| TATCTACTTTATGGTCCTCC+AGG | - | Chr6:7287130-7287149 | MsG0680030694.01.T01:CDS | 40.0% | |
| TCAAGAGGAAGAAAAGGTGA+AGG | - | Chr6:7287864-7287883 | MsG0680030694.01.T01:CDS | 40.0% | |
| TCAATGGAGTCAAAGTTTGG+TGG | - | Chr6:7286732-7286751 | MsG0680030694.01.T01:CDS | 40.0% | |
| TCATCCATGCTAAGAACAAG+TGG | + | Chr6:7286689-7286708 | None:intergenic | 40.0% | |
| TGAGGAGGAAGCAAAGAAAA+AGG | - | Chr6:7287789-7287808 | MsG0680030694.01.T01:CDS | 40.0% | |
| TTTGAGCATCCTGCAAGATT+TGG | - | Chr6:7286998-7287017 | MsG0680030694.01.T01:CDS | 40.0% | |
| ! | GAGAATGGTGTCAAAGAGAA+TGG | - | Chr6:7287916-7287935 | MsG0680030694.01.T01:CDS | 40.0% |
| !!! | TCTTTTCTGGTTCCATAGCT+AGG | + | Chr6:7287027-7287046 | None:intergenic | 40.0% |
| ACCCCCACCAATTACTACTA+GGG | + | Chr6:7286949-7286968 | None:intergenic | 45.0% | |
| ACCCTAGTAGTAATTGGTGG+GGG | - | Chr6:7286945-7286964 | MsG0680030694.01.T01:CDS | 45.0% | |
| AGATGGGAATTGGGGAACTA+TGG | - | Chr6:7286393-7286412 | MsG0680030694.01.T01:CDS | 45.0% | |
| ATTGTTCTCTCGATCTCACC+GGG | - | Chr6:7287305-7287324 | MsG0680030694.01.T01:CDS | 45.0% | |
| GAGGAAAAGGTGAAGGAGAA+TGG | - | Chr6:7287901-7287920 | MsG0680030694.01.T01:CDS | 45.0% | |
| GATGGAATTTGGTCAGCTTG+TGG | - | Chr6:7287454-7287473 | MsG0680030694.01.T01:CDS | 45.0% | |
| GATTGTTCTCTCGATCTCAC+CGG | - | Chr6:7287304-7287323 | MsG0680030694.01.T01:CDS | 45.0% | |
| GCTAGGTTTCCAAATCTTGC+AGG | + | Chr6:7287010-7287029 | None:intergenic | 45.0% | |
| TACCCCCACCAATTACTACT+AGG | + | Chr6:7286950-7286969 | None:intergenic | 45.0% | |
| TCTCATAAGGAGGGGAAGAA+TGG | - | Chr6:7287531-7287550 | MsG0680030694.01.T01:CDS | 45.0% | |
| TGATCCTGCTCTCATAAGGA+GGG | - | Chr6:7287522-7287541 | MsG0680030694.01.T01:CDS | 45.0% | |
| TTGATCCTGCTCTCATAAGG+AGG | - | Chr6:7287521-7287540 | MsG0680030694.01.T01:CDS | 45.0% | |
| ! | GGGGGTATAAAAGCACAAAG+TGG | - | Chr6:7286963-7286982 | MsG0680030694.01.T01:CDS | 45.0% |
| ! | GTAGATTTTCCAGTTCCTGG+AGG | + | Chr6:7287148-7287167 | None:intergenic | 45.0% |
| CTTTATGGTCCTCCAGGAAC+TGG | - | Chr6:7287136-7287155 | MsG0680030694.01.T01:CDS | 50.0% | |
| GATCCTGCTCTCATAAGGAG+GGG | - | Chr6:7287523-7287542 | MsG0680030694.01.T01:CDS | 50.0% | |
| GGATGAGGAAGCAAAGCTCA+AGG | - | Chr6:7287816-7287835 | MsG0680030694.01.T01:CDS | 50.0% | |
| GGGGAACTATGGTCACAACT+AGG | - | Chr6:7286404-7286423 | MsG0680030694.01.T01:CDS | 50.0% | |
| GTTGGAAAGGCTTGGAAACG+TGG | - | Chr6:7287106-7287125 | MsG0680030694.01.T01:CDS | 50.0% | |
| CTTCCCCTCCTTATGAGAGC+AGG | + | Chr6:7287529-7287548 | None:intergenic | 55.0% | |
| GGTCAGCTTGTGGATCAGAG+AGG | - | Chr6:7287464-7287483 | MsG0680030694.01.T01:CDS | 55.0% | |
| CTCTCGATCTCACCGGGCAA+AGG | - | Chr6:7287311-7287330 | MsG0680030694.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 7286266 | 7287966 | 7286266 | ID=MsG0680030694.01;Name=MsG0680030694.01 |
| Chr6 | mRNA | 7286266 | 7287966 | 7286266 | ID=MsG0680030694.01.T01;Parent=MsG0680030694.01;Name=MsG0680030694.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|566 |
| Chr6 | exon | 7286266 | 7287966 | 7286266 | ID=MsG0680030694.01.T01:exon:6719;Parent=MsG0680030694.01.T01 |
| Chr6 | CDS | 7286266 | 7287966 | 7286266 | ID=MsG0680030694.01.T01:cds;Parent=MsG0680030694.01.T01 |
| Gene Sequence |
| Protein sequence |
