AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024402.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024402.01.T01 MTR_1g104555 90.991 111 10 0 25 135 564 674 2.75e-70 223
MsG0580024402.01.T01 MTR_5g006160 55.963 109 38 2 25 133 672 770 3.83e-34 125
MsG0580024402.01.T01 MTR_2g067970 43.925 107 55 3 25 131 785 886 9.16e-24 95.9
MsG0580024402.01.T01 MTR_2g067990 43.925 107 55 2 25 131 897 998 3.18e-22 91.7
MsG0580024402.01.T01 MTR_2g067980 43.925 107 55 2 25 131 921 1022 5.59e-22 90.9
MsG0580024402.01.T01 MTR_2g068650 42.991 107 56 2 25 131 635 736 1.20e-21 90.1
MsG0580024402.01.T01 MTR_5g013070 40.187 107 60 2 25 131 692 794 2.91e-21 89.0
MsG0580024402.01.T01 MTR_5g013130 39.252 107 61 2 25 131 689 791 5.32e-20 85.1
MsG0580024402.01.T01 MTR_8g011370 40.187 107 60 1 25 131 702 804 3.11e-19 83.2
MsG0580024402.01.T01 MTR_8g011410 37.190 121 65 3 13 127 687 802 8.79e-17 75.9
MsG0580024402.01.T01 MTR_4g114280 36.792 106 62 2 26 131 734 834 1.51e-16 75.5
MsG0580024402.01.T01 MTR_4g114240 39.623 106 59 2 26 131 720 820 1.04e-15 72.8
MsG0580024402.01.T01 MTR_4g114270 32.110 109 66 3 26 134 706 806 7.44e-15 70.5
MsG0580024402.01.T01 MTR_8g067720 34.746 118 66 4 17 132 511 619 1.96e-14 69.3
MsG0580024402.01.T01 MTR_8g011440 33.871 124 71 3 13 130 699 817 3.10e-14 68.9
MsG0580024402.01.T01 MTR_8g067630 34.746 118 66 4 17 132 460 568 7.36e-14 67.8
MsG0580024402.01.T01 MTR_4g114250 31.132 106 65 3 26 131 708 805 1.83e-13 66.6
MsG0580024402.01.T01 MTR_3g107070 32.000 125 72 3 13 131 636 753 3.45e-13 65.9
MsG0580024402.01.T01 MTR_7g116130 31.731 104 65 1 17 120 528 625 3.47e-13 65.9
MsG0580024402.01.T01 MTR_6g083780 35.514 107 60 3 26 131 537 635 4.72e-13 65.5
MsG0580024402.01.T01 MTR_1g028020 33.645 107 63 3 26 131 331 430 6.48e-13 65.1
MsG0580024402.01.T01 MTR_8g067930 35.514 107 58 4 28 132 523 620 6.49e-13 65.1
MsG0580024402.01.T01 MTR_1g027320 33.654 104 55 3 26 126 186 278 4.06e-12 62.4
MsG0580024402.01.T01 MTR_4g113710 35.345 116 57 5 26 131 289 396 4.15e-12 62.4
MsG0580024402.01.T01 MTR_2g008390 31.481 108 70 1 17 124 468 571 5.89e-12 62.0
MsG0580024402.01.T01 MTR_7g082280 33.898 118 64 6 26 137 611 720 5.97e-12 62.0
MsG0580024402.01.T01 MTR_1g097160 32.407 108 69 1 17 124 478 581 6.96e-12 62.0
MsG0580024402.01.T01 MTR_7g082300 34.259 108 61 5 26 131 734 833 7.73e-12 62.0
MsG0580024402.01.T01 MTR_5g033820 29.630 108 71 1 17 124 476 578 7.81e-12 61.6
MsG0580024402.01.T01 MTR_2g008370 34.343 99 60 1 26 124 472 565 7.95e-12 61.6
MsG0580024402.01.T01 MTR_8g068050 35.238 105 60 2 28 132 538 634 8.45e-12 61.6
MsG0580024402.01.T01 MTR_8g068050 35.238 105 60 2 28 132 428 524 1.01e-11 61.6
MsG0580024402.01.T01 MTR_1g028100 32.710 107 64 3 26 131 273 372 1.05e-11 61.2
MsG0580024402.01.T01 MTR_1g028170 32.710 107 64 3 26 131 300 399 1.06e-11 61.2
MsG0580024402.01.T01 MTR_7g085860 33.945 109 59 5 26 131 349 447 1.22e-11 61.2
MsG0580024402.01.T01 MTR_2g008360 31.481 108 70 1 17 124 467 570 1.72e-11 60.8
MsG0580024402.01.T01 MTR_5g077100 33.962 106 69 1 26 131 549 653 1.93e-11 60.8
MsG0580024402.01.T01 MTR_7g082110 32.743 113 58 6 25 131 719 819 2.03e-11 60.5
MsG0580024402.01.T01 MTR_8g088760 34.259 108 63 3 26 130 471 573 2.07e-11 60.5
MsG0580024402.01.T01 MTR_1g029610 31.132 106 66 1 26 131 474 572 2.10e-11 60.5
MsG0580024402.01.T01 MTR_7g018200 31.481 108 69 1 17 124 476 578 2.13e-11 60.5
MsG0580024402.01.T01 MTR_2g036460 31.008 129 76 4 10 132 304 425 2.57e-11 60.1
MsG0580024402.01.T01 MTR_2g054870 33.043 115 74 2 20 131 284 398 2.88e-11 60.1
MsG0580024402.01.T01 MTR_7g115740 31.897 116 71 4 17 132 531 638 9.70e-11 58.5
MsG0580024402.01.T01 MTR_7g082400 33.945 109 61 5 26 131 268 368 9.87e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024402.01.T01 AT1G70250 29.907 107 71 2 26 131 642 745 2.02e-13 66.6
MsG0580024402.01.T01 AT1G60800 29.630 108 71 1 17 124 408 510 3.84e-13 65.5
MsG0580024402.01.T01 AT1G60800 29.630 108 71 1 17 124 475 577 4.92e-13 65.5
MsG0580024402.01.T01 AT1G71830 32.407 108 69 1 17 124 476 579 1.49e-12 63.9
MsG0580024402.01.T01 AT1G34210 32.407 108 69 1 17 124 479 582 2.08e-12 63.5
MsG0580024402.01.T01 AT1G34210 32.407 108 69 1 17 124 479 582 2.08e-12 63.5
MsG0580024402.01.T01 AT4G33430 33.333 108 68 1 17 124 510 613 3.63e-12 62.8
MsG0580024402.01.T01 AT4G33430 33.333 108 68 1 17 124 463 566 3.80e-12 62.8
MsG0580024402.01.T01 AT5G03140 33.645 107 70 1 26 132 553 658 4.36e-12 62.8
MsG0580024402.01.T01 AT4G32300 32.031 128 69 5 13 131 656 774 7.39e-12 62.0
MsG0580024402.01.T01 AT1G29730 32.353 102 64 1 26 127 821 917 7.66e-12 62.0
MsG0580024402.01.T01 AT1G29730 32.353 102 64 1 26 127 853 949 7.68e-12 62.0
MsG0580024402.01.T01 AT1G66980 34.821 112 59 6 26 131 970 1073 1.61e-11 60.8
MsG0580024402.01.T01 AT1G66980 34.821 112 59 6 26 131 987 1090 1.61e-11 60.8
MsG0580024402.01.T01 AT1G66980 34.821 112 59 6 26 131 1014 1117 1.62e-11 60.8
MsG0580024402.01.T01 AT1G66980 34.821 112 59 6 26 131 1016 1119 1.62e-11 60.8
MsG0580024402.01.T01 AT4G18250 35.455 110 63 4 26 131 539 644 1.90e-11 60.8
MsG0580024402.01.T01 AT3G25560 32.407 108 68 1 17 124 482 584 1.94e-11 60.8
MsG0580024402.01.T01 AT3G25560 32.407 108 68 1 17 124 483 585 1.94e-11 60.8
MsG0580024402.01.T01 AT3G25560 32.407 108 68 1 17 124 482 584 1.94e-11 60.8
MsG0580024402.01.T01 AT1G67000 28.947 114 71 4 26 131 739 850 3.36e-11 60.1
MsG0580024402.01.T01 AT1G29740 33.684 95 58 1 26 120 857 946 5.40e-11 59.7
MsG0580024402.01.T01 AT1G29740 33.684 95 58 1 26 120 857 946 5.43e-11 59.7
MsG0580024402.01.T01 AT3G53380 33.962 106 70 0 26 131 556 661 6.64e-11 59.3
MsG0580024402.01.T01 AT5G38280 29.907 107 70 3 26 131 514 616 7.75e-11 58.9
MsG0580024402.01.T01 AT5G38280 29.907 107 70 3 26 131 514 616 7.76e-11 58.9
MsG0580024402.01.T01 AT1G53430 33.028 109 62 4 26 132 810 909 8.41e-11 58.9
MsG0580024402.01.T01 AT1G53430 33.028 109 62 4 26 132 851 950 8.44e-11 58.9
MsG0580024402.01.T01 AT4G02420 29.630 108 71 1 17 124 522 624 8.46e-11 58.9

Find 33 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATAGATTGGGTTCTATAT+TGG 0.218702 5:+4346055 MsG0580024402.01.T01:CDS
TAGAGGGACAATGGGATATT+TGG 0.257183 5:+4345872 MsG0580024402.01.T01:CDS
TATAGATTGGGTTCTATATT+GGG 0.279264 5:+4346056 MsG0580024402.01.T01:CDS
GATGGCCTAATAGTTGGATT+AGG 0.320977 5:-4346183 None:intergenic
TGCAACACCTTTGCAATAGA+TGG 0.340385 5:-4346201 None:intergenic
ATGACATGATACTTATAGAT+TGG 0.369608 5:+4346043 MsG0580024402.01.T01:CDS
AAGTCGTTAATGATTTCATT+AGG 0.378871 5:+4346121 MsG0580024402.01.T01:CDS
TGTGCCCTAATCCAACTATT+AGG 0.379826 5:+4346178 MsG0580024402.01.T01:CDS
AGTGTTCCACCATTGTTTGA+TGG 0.394571 5:+4346249 MsG0580024402.01.T01:CDS
GCAATAGATGGCCTAATAGT+TGG 0.413635 5:-4346189 None:intergenic
ATGGCCTAATAGTTGGATTA+GGG 0.430405 5:-4346182 None:intergenic
TAGGTTTGAGAGAATGACTA+TGG 0.451333 5:+4346140 MsG0580024402.01.T01:CDS
TGACATGATACTTATAGATT+GGG 0.452945 5:+4346044 MsG0580024402.01.T01:CDS
GGTTCTATATTGGGCAAAAG+AGG 0.465154 5:+4346065 MsG0580024402.01.T01:CDS
AGAACAAGCACAAATGTTAG+AGG 0.472788 5:+4345855 None:intergenic
GCAAAAGTGGATTGCAAAAG+TGG 0.476992 5:+4345927 MsG0580024402.01.T01:CDS
GAGTTGCATCAAGTTGAAGA+TGG 0.493345 5:+4346006 MsG0580024402.01.T01:CDS
TGCACCTATCACTGCAAAAG+TGG 0.498118 5:+4345914 MsG0580024402.01.T01:CDS
AAAGTGGATATCTACAGCTT+TGG 0.498218 5:+4345943 MsG0580024402.01.T01:CDS
ACAAATGTTAGAGGGACAAT+GGG 0.506091 5:+4345864 None:intergenic
TTTGAGAGAATGACTATGGT+TGG 0.507037 5:+4346144 MsG0580024402.01.T01:CDS
GTTCTATATTGGGCAAAAGA+GGG 0.512150 5:+4346066 MsG0580024402.01.T01:CDS
CACAAATGTTAGAGGGACAA+TGG 0.540064 5:+4345863 None:intergenic
TGCAAAGGTGTTGCAGATGT+CGG 0.550893 5:+4346209 MsG0580024402.01.T01:CDS
CATTAACGACTTCAACATCA+TGG 0.554782 5:-4346110 None:intergenic
CAAGTTGAAGATGGAACAGA+AGG 0.575586 5:+4346015 MsG0580024402.01.T01:CDS
TGGGATATTTGGCACCAGAA+TGG 0.579107 5:+4345883 MsG0580024402.01.T01:CDS
TTCTATATTGGGCAAAAGAG+GGG 0.600711 5:+4346067 MsG0580024402.01.T01:CDS
AAGGTGTTGCAGATGTCGGA+AGG 0.614948 5:+4346213 MsG0580024402.01.T01:CDS
TATTAGGCCATCTATTGCAA+AGG 0.622506 5:+4346194 MsG0580024402.01.T01:CDS
GAACAAGCACAAATGTTAGA+GGG 0.627687 5:+4345856 None:intergenic
GTTGAAGATGGAACAGAAGG+TGG 0.634859 5:+4346018 MsG0580024402.01.T01:CDS
GAATGACTATGGTTGGACTG+TGG 0.741878 5:+4346151 MsG0580024402.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTTTTCCATCAAACAA+TGG - Chr5:4346261-4346280 None:intergenic 20.0%
!!! TTGAAATCTTATTTTGTAGA+AGG + Chr5:4345977-4345996 MsG0580024402.01.T01:CDS 20.0%
! AAGTCGTTAATGATTTCATT+AGG + Chr5:4346121-4346140 MsG0580024402.01.T01:CDS 25.0%
! ATGACATGATACTTATAGAT+TGG + Chr5:4346043-4346062 MsG0580024402.01.T01:CDS 25.0%
! TATAGATTGGGTTCTATATT+GGG + Chr5:4346056-4346075 MsG0580024402.01.T01:CDS 25.0%
! TGACATGATACTTATAGATT+GGG + Chr5:4346044-4346063 MsG0580024402.01.T01:CDS 25.0%
! TTATAGATTGGGTTCTATAT+TGG + Chr5:4346055-4346074 MsG0580024402.01.T01:CDS 25.0%
! ATTTTTCCATCAAACAATGG+TGG - Chr5:4346258-4346277 None:intergenic 30.0%
AAAGTGGATATCTACAGCTT+TGG + Chr5:4345943-4345962 MsG0580024402.01.T01:CDS 35.0%
ATGGCCTAATAGTTGGATTA+GGG - Chr5:4346185-4346204 None:intergenic 35.0%
CATTAACGACTTCAACATCA+TGG - Chr5:4346113-4346132 None:intergenic 35.0%
GTTCTATATTGGGCAAAAGA+GGG + Chr5:4346066-4346085 MsG0580024402.01.T01:CDS 35.0%
TAGGTTTGAGAGAATGACTA+TGG + Chr5:4346140-4346159 MsG0580024402.01.T01:CDS 35.0%
TATTAGGCCATCTATTGCAA+AGG + Chr5:4346194-4346213 MsG0580024402.01.T01:CDS 35.0%
TTCTATATTGGGCAAAAGAG+GGG + Chr5:4346067-4346086 MsG0580024402.01.T01:CDS 35.0%
TTTGAGAGAATGACTATGGT+TGG + Chr5:4346144-4346163 MsG0580024402.01.T01:CDS 35.0%
AGTGTTCCACCATTGTTTGA+TGG + Chr5:4346249-4346268 MsG0580024402.01.T01:CDS 40.0%
GATGGCCTAATAGTTGGATT+AGG - Chr5:4346186-4346205 None:intergenic 40.0%
GCAATAGATGGCCTAATAGT+TGG - Chr5:4346192-4346211 None:intergenic 40.0%
GGTTCTATATTGGGCAAAAG+AGG + Chr5:4346065-4346084 MsG0580024402.01.T01:CDS 40.0%
TAGAGGGACAATGGGATATT+TGG + Chr5:4345872-4345891 MsG0580024402.01.T01:CDS 40.0%
TGCAACACCTTTGCAATAGA+TGG - Chr5:4346204-4346223 None:intergenic 40.0%
TGTGCCCTAATCCAACTATT+AGG + Chr5:4346178-4346197 MsG0580024402.01.T01:CDS 40.0%
! CAAGTTGAAGATGGAACAGA+AGG + Chr5:4346015-4346034 MsG0580024402.01.T01:CDS 40.0%
!! CAATCCACTTTTGCAGTGAT+AGG - Chr5:4345921-4345940 None:intergenic 40.0%
!! GAGTTGCATCAAGTTGAAGA+TGG + Chr5:4346006-4346025 MsG0580024402.01.T01:CDS 40.0%
!! GCAAAAGTGGATTGCAAAAG+TGG + Chr5:4345927-4345946 MsG0580024402.01.T01:CDS 40.0%
GAATGACTATGGTTGGACTG+TGG + Chr5:4346151-4346170 MsG0580024402.01.T01:CDS 45.0%
GTTGAAGATGGAACAGAAGG+TGG + Chr5:4346018-4346037 MsG0580024402.01.T01:CDS 45.0%
TGCACCTATCACTGCAAAAG+TGG + Chr5:4345914-4345933 MsG0580024402.01.T01:CDS 45.0%
TGGGATATTTGGCACCAGAA+TGG + Chr5:4345883-4345902 MsG0580024402.01.T01:CDS 45.0%
!! GGTGCATTTTTGAGCCATTC+TGG - Chr5:4345900-4345919 None:intergenic 45.0%
!! TGCAAAGGTGTTGCAGATGT+CGG + Chr5:4346209-4346228 MsG0580024402.01.T01:CDS 45.0%
!! AAGGTGTTGCAGATGTCGGA+AGG + Chr5:4346213-4346232 MsG0580024402.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr5 gene 4345868 4346284 4345868 ID=MsG0580024402.01;Name=MsG0580024402.01
Chr5 mRNA 4345868 4346284 4345868 ID=MsG0580024402.01.T01;Parent=MsG0580024402.01;Name=MsG0580024402.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|138
Chr5 exon 4345868 4346284 4345868 ID=MsG0580024402.01.T01:exon:4470;Parent=MsG0580024402.01.T01
Chr5 CDS 4345868 4346284 4345868 ID=MsG0580024402.01.T01:cds;Parent=MsG0580024402.01.T01
Gene Sequence

>MsG0580024402.01.T01

ATGTTAGAGGGACAATGGGATATTTGGCACCAGAATGGCTCAAAAATGCACCTATCACTGCAAAAGTGGATTGCAAAAGTGGATATCTACAGCTTTGGTGTCATGTTGCTTGAAATCTTATTTTGTAGAAGGCACATTGAGTTGCATCAAGTTGAAGATGGAACAGAAGGTGGTGATGACATGATACTTATAGATTGGGTTCTATATTGGGCAAAAGAGGGGAAACTAAGAGACATTCTTAGCCATGATGTTGAAGTCGTTAATGATTTCATTAGGTTTGAGAGAATGACTATGGTTGGACTGTGGTGCTTGTGCCCTAATCCAACTATTAGGCCATCTATTGCAAAGGTGTTGCAGATGTCGGAAGGAGATTTTGAAGTTAGTGTTCCACCATTGTTTGATGGAAAAATTTTGTAA

Protein sequence

>MsG0580024402.01.T01

MLEGQWDIWHQNGSKMHLSLQKWIAKVDIYSFGVMLLEILFCRRHIELHQVEDGTEGGDDMILIDWVLYWAKEGKLRDILSHDVEVVNDFIRFERMTMVGLWCLCPNPTIRPSIAKVLQMSEGDFEVSVPPLFDGKIL*