Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024533.01.T01 | XP_039689995.1 | 91.558 | 154 | 13 | 0 | 21 | 174 | 321 | 474 | 1.32E-95 | 295 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024533.01.T01 | G7JXC4 | 88.272 | 162 | 18 | 1 | 14 | 174 | 430 | 591 | 1.06e-94 | 295 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000292.01 | MsG0580024533.01 | 0.806638 | 7.463738e-50 | 4.071648e-47 |
| MsG0180004103.01 | MsG0580024533.01 | 0.815511 | 8.905711e-52 | 6.152554e-49 |
| MsG0180005485.01 | MsG0580024533.01 | 0.805109 | 1.564227e-49 | 8.205989e-47 |
| MsG0180005807.01 | MsG0580024533.01 | 0.838648 | 2.538049e-57 | 3.421507e-54 |
| MsG0280010569.01 | MsG0580024533.01 | 0.806361 | 8.540130e-50 | 4.626085e-47 |
| MsG0280010611.01 | MsG0580024533.01 | 0.868403 | 6.701455e-66 | 2.420843e-62 |
| MsG0380014386.01 | MsG0580024533.01 | 0.807818 | 4.197081e-50 | 2.360940e-47 |
| MsG0380014909.01 | MsG0580024533.01 | 0.814133 | 1.799249e-51 | 1.197334e-48 |
| MsG0480020315.01 | MsG0580024533.01 | 0.817764 | 2.784470e-52 | 2.046055e-49 |
| MsG0480021060.01 | MsG0580024533.01 | 0.838944 | 2.128658e-57 | 2.895259e-54 |
| MsG0480023452.01 | MsG0580024533.01 | 0.802019 | 6.842283e-49 | 3.316081e-46 |
| MsG0480023553.01 | MsG0580024533.01 | 0.807900 | 4.033129e-50 | 2.273436e-47 |
| MsG0580024533.01 | MsG0580026179.01 | 0.803806 | 2.923617e-49 | 1.483135e-46 |
| MsG0580024533.01 | MsG0580026271.01 | 0.822277 | 2.581404e-53 | 2.150785e-50 |
| MsG0580024533.01 | MsG0580029529.01 | 0.805546 | 1.266860e-49 | 6.720881e-47 |
| MsG0580024533.01 | MsG0680030680.01 | 0.827223 | 1.761437e-54 | 1.689869e-51 |
| MsG0580024533.01 | MsG0680030908.01 | 0.817040 | 4.052685e-52 | 2.919429e-49 |
| MsG0580024533.01 | MsG0680032254.01 | 0.812454 | 4.205836e-51 | 2.675452e-48 |
| MsG0580024533.01 | MsG0680032413.01 | 0.801542 | 8.571302e-49 | 4.104116e-46 |
| MsG0580024533.01 | MsG0780036243.01 | 0.814405 | 1.566509e-51 | 1.050247e-48 |
| MsG0580024533.01 | MsG0780040278.01 | 0.842039 | 3.297251e-58 | 4.936697e-55 |
| MsG0580024533.01 | MsG0880043249.01 | 0.816522 | 5.296461e-52 | 3.761570e-49 |
| MsG0580024533.01 | MsG0880045558.01 | 0.811656 | 6.278609e-51 | 3.908600e-48 |
| MsG0580024533.01 | MsG0880045668.01 | 0.828446 | 8.948169e-55 | 8.896679e-52 |
| MsG0580024533.01 | MsG0880047770.01 | 0.803728 | 3.034902e-49 | 1.536537e-46 |
| MsG0380015896.01 | MsG0580024533.01 | 0.815931 | 7.179523e-52 | 5.017050e-49 |
| MsG0280007075.01 | MsG0580024533.01 | 0.804574 | 2.023832e-49 | 1.047141e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024533.01.T01 | MTR_5g012790 | 88.272 | 162 | 18 | 1 | 14 | 174 | 430 | 591 | 2.68e-98 | 295 |
| MsG0580024533.01.T01 | MTR_8g078760 | 33.113 | 151 | 92 | 2 | 21 | 171 | 484 | 625 | 2.02e-25 | 102 |
| MsG0580024533.01.T01 | MTR_5g018820 | 32.450 | 151 | 94 | 2 | 21 | 171 | 481 | 623 | 2.87e-24 | 99.0 |
| MsG0580024533.01.T01 | MTR_4g068920 | 32.308 | 130 | 84 | 1 | 42 | 171 | 508 | 633 | 1.83e-21 | 90.9 |
| MsG0580024533.01.T01 | MTR_8g079840 | 30.894 | 123 | 85 | 0 | 46 | 168 | 475 | 597 | 4.91e-20 | 86.7 |
| MsG0580024533.01.T01 | MTR_5g017790 | 30.894 | 123 | 85 | 0 | 46 | 168 | 412 | 534 | 2.76e-18 | 81.6 |
| MsG0580024533.01.T01 | MTR_5g017790 | 30.894 | 123 | 85 | 0 | 46 | 168 | 424 | 546 | 2.98e-18 | 81.6 |
| MsG0580024533.01.T01 | MTR_1g021040 | 33.333 | 120 | 79 | 1 | 47 | 166 | 427 | 545 | 6.83e-18 | 80.5 |
| MsG0580024533.01.T01 | MTR_3g101340 | 31.746 | 126 | 85 | 1 | 42 | 167 | 414 | 538 | 8.98e-18 | 80.1 |
| MsG0580024533.01.T01 | MTR_3g101340 | 31.746 | 126 | 85 | 1 | 42 | 167 | 447 | 571 | 9.89e-18 | 80.1 |
| MsG0580024533.01.T01 | MTR_3g101340 | 31.746 | 126 | 85 | 1 | 42 | 167 | 440 | 564 | 1.04e-17 | 80.1 |
| MsG0580024533.01.T01 | MTR_5g079910 | 28.788 | 132 | 92 | 1 | 37 | 166 | 378 | 509 | 1.07e-17 | 80.1 |
| MsG0580024533.01.T01 | MTR_4g058015 | 27.891 | 147 | 102 | 3 | 25 | 167 | 544 | 690 | 3.59e-14 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024533.01.T01 | AT5G47380 | 59.740 | 154 | 59 | 2 | 21 | 173 | 386 | 537 | 6.20e-62 | 200 |
| MsG0580024533.01.T01 | AT5G47380 | 59.740 | 154 | 59 | 2 | 21 | 173 | 442 | 593 | 2.25e-61 | 200 |
| MsG0580024533.01.T01 | AT5G47380 | 59.740 | 154 | 59 | 2 | 21 | 173 | 442 | 593 | 2.33e-61 | 200 |
| MsG0580024533.01.T01 | AT4G37080 | 32.353 | 136 | 92 | 0 | 33 | 168 | 462 | 597 | 1.08e-22 | 94.4 |
| MsG0580024533.01.T01 | AT4G37080 | 32.353 | 136 | 92 | 0 | 33 | 168 | 475 | 610 | 1.19e-22 | 94.4 |
| MsG0580024533.01.T01 | AT4G37080 | 32.353 | 136 | 92 | 0 | 33 | 168 | 475 | 610 | 1.19e-22 | 94.4 |
| MsG0580024533.01.T01 | AT2G23700 | 31.579 | 152 | 94 | 4 | 21 | 171 | 564 | 706 | 2.27e-22 | 93.6 |
| MsG0580024533.01.T01 | AT5G66600 | 32.258 | 155 | 93 | 5 | 13 | 165 | 463 | 607 | 5.63e-22 | 92.4 |
| MsG0580024533.01.T01 | AT5G66600 | 32.258 | 155 | 93 | 5 | 13 | 165 | 463 | 607 | 5.63e-22 | 92.4 |
| MsG0580024533.01.T01 | AT5G66600 | 32.258 | 155 | 93 | 5 | 13 | 165 | 463 | 607 | 5.63e-22 | 92.4 |
| MsG0580024533.01.T01 | AT5G66600 | 32.258 | 155 | 93 | 5 | 13 | 165 | 463 | 607 | 5.63e-22 | 92.4 |
| MsG0580024533.01.T01 | AT5G66600 | 32.258 | 155 | 93 | 5 | 13 | 165 | 463 | 607 | 5.63e-22 | 92.4 |
| MsG0580024533.01.T01 | AT5G66600 | 32.258 | 155 | 93 | 5 | 13 | 165 | 443 | 587 | 5.89e-22 | 92.4 |
| MsG0580024533.01.T01 | AT5G66600 | 32.258 | 155 | 93 | 5 | 13 | 165 | 409 | 553 | 6.23e-22 | 92.0 |
| MsG0580024533.01.T01 | AT5G42690 | 34.559 | 136 | 87 | 2 | 33 | 167 | 353 | 487 | 6.07e-20 | 86.3 |
| MsG0580024533.01.T01 | AT5G42690 | 34.559 | 136 | 87 | 2 | 33 | 167 | 404 | 538 | 8.00e-20 | 86.3 |
| MsG0580024533.01.T01 | AT5G42690 | 34.559 | 136 | 87 | 2 | 33 | 167 | 378 | 512 | 8.07e-20 | 85.9 |
| MsG0580024533.01.T01 | AT5G42690 | 34.559 | 136 | 87 | 2 | 33 | 167 | 405 | 539 | 8.09e-20 | 86.3 |
| MsG0580024533.01.T01 | AT5G42690 | 34.559 | 136 | 87 | 2 | 33 | 167 | 405 | 539 | 8.09e-20 | 86.3 |
| MsG0580024533.01.T01 | AT2G39690 | 30.827 | 133 | 90 | 1 | 37 | 167 | 285 | 417 | 7.28e-19 | 83.2 |
| MsG0580024533.01.T01 | AT2G39690 | 30.827 | 133 | 90 | 1 | 37 | 167 | 366 | 498 | 8.75e-19 | 83.2 |
| MsG0580024533.01.T01 | AT2G39690 | 30.827 | 133 | 90 | 1 | 37 | 167 | 365 | 497 | 9.35e-19 | 82.8 |
| MsG0580024533.01.T01 | AT2G39690 | 30.827 | 133 | 90 | 1 | 37 | 167 | 391 | 523 | 1.07e-18 | 82.8 |
| MsG0580024533.01.T01 | AT2G39690 | 30.827 | 133 | 90 | 1 | 37 | 167 | 392 | 524 | 1.08e-18 | 82.8 |
| MsG0580024533.01.T01 | AT1G43020 | 33.058 | 121 | 77 | 1 | 51 | 171 | 323 | 439 | 2.12e-18 | 82.0 |
| MsG0580024533.01.T01 | AT1G16750 | 32.867 | 143 | 76 | 5 | 33 | 166 | 396 | 527 | 2.42e-18 | 82.0 |
| MsG0580024533.01.T01 | AT3G18900 | 31.405 | 121 | 80 | 1 | 42 | 162 | 328 | 445 | 1.34e-17 | 79.7 |
| MsG0580024533.01.T01 | AT3G18900 | 31.405 | 121 | 80 | 1 | 42 | 162 | 328 | 445 | 1.34e-17 | 79.7 |
| MsG0580024533.01.T01 | AT3G18900 | 31.405 | 121 | 80 | 1 | 42 | 162 | 409 | 526 | 1.73e-17 | 79.3 |
| MsG0580024533.01.T01 | AT3G18900 | 31.405 | 121 | 80 | 1 | 42 | 162 | 404 | 521 | 1.76e-17 | 79.3 |
| MsG0580024533.01.T01 | AT1G76620 | 26.174 | 149 | 102 | 2 | 23 | 171 | 381 | 521 | 4.97e-16 | 75.1 |
| MsG0580024533.01.T01 | AT3G13000 | 32.258 | 124 | 80 | 3 | 48 | 169 | 456 | 577 | 7.23e-15 | 72.0 |
| MsG0580024533.01.T01 | AT3G13000 | 32.258 | 124 | 80 | 3 | 48 | 169 | 456 | 577 | 7.23e-15 | 72.0 |
| MsG0580024533.01.T01 | AT3G13000 | 32.258 | 124 | 80 | 3 | 48 | 169 | 427 | 548 | 7.94e-15 | 71.6 |
| MsG0580024533.01.T01 | AT1G21060 | 35.088 | 114 | 69 | 2 | 52 | 165 | 394 | 502 | 1.25e-13 | 68.2 |
| MsG0580024533.01.T01 | AT1G21060 | 35.088 | 114 | 69 | 2 | 52 | 165 | 382 | 490 | 1.29e-13 | 68.2 |
| MsG0580024533.01.T01 | AT3G12540 | 24.638 | 138 | 99 | 1 | 35 | 167 | 367 | 504 | 1.69e-12 | 65.1 |
| MsG0580024533.01.T01 | AT3G12540 | 24.638 | 138 | 99 | 1 | 35 | 167 | 434 | 571 | 2.47e-12 | 64.7 |
Find 26 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAATCTTTCCACCAATTT+AGG | 0.083497 | 5:+6109030 | None:intergenic |
| ACAACTCATCAACTCAAATT+TGG | 0.154234 | 5:-6109450 | MsG0580024533.01.T01:CDS |
| AAAATCTTTCCACCAATTTA+GGG | 0.275012 | 5:+6109031 | None:intergenic |
| TAAAGTCATTCTCCCTAAAT+TGG | 0.293856 | 5:-6109043 | MsG0580024533.01.T01:CDS |
| GGCCTTACAGTTCAAAATTC+AGG | 0.310105 | 5:-6108873 | MsG0580024533.01.T01:CDS |
| CAACTCATCAACTCAAATTT+GGG | 0.385152 | 5:-6109449 | MsG0580024533.01.T01:CDS |
| CCTTTGCACCGGAGCATTGT+CGG | 0.387970 | 5:-6109393 | MsG0580024533.01.T01:CDS |
| GTCTGCTTTGCCCTTTGCAC+CGG | 0.407525 | 5:-6109404 | MsG0580024533.01.T01:CDS |
| CGGAGCATTGTCGGATCCTA+TGG | 0.417025 | 5:-6109384 | MsG0580024533.01.T01:intron |
| TGATATCCTTGGATGGATAA+TGG | 0.425516 | 5:-6108983 | MsG0580024533.01.T01:CDS |
| ATCAGCATTGATGATATCCT+TGG | 0.479859 | 5:-6108994 | MsG0580024533.01.T01:CDS |
| CGACAATGCTCCGGTGCAAA+GGG | 0.482820 | 5:+6109394 | None:intergenic |
| TGTCTGTAGAAACTCTCTCT+TGG | 0.502312 | 5:+6109089 | None:intergenic |
| CCGACAATGCTCCGGTGCAA+AGG | 0.512555 | 5:+6109393 | None:intergenic |
| GGCAAAGCAGACAAGTGGTT+GGG | 0.513513 | 5:+6109415 | None:intergenic |
| ATTGTAGCTAAAAGTTTACT+CGG | 0.519041 | 5:-6109142 | MsG0580024533.01.T01:intron |
| GTGTTGTGTCTGCTGCCCTT+AGG | 0.521903 | 5:-6109493 | MsG0580024533.01.T01:CDS |
| AGTCATTCTCCCTAAATTGG+TGG | 0.528274 | 5:-6109040 | MsG0580024533.01.T01:CDS |
| CATAGGATCCGACAATGCTC+CGG | 0.547311 | 5:+6109385 | None:intergenic |
| TTCGAATCTAAGAGAAGAGC+TGG | 0.561093 | 5:-6109118 | MsG0580024533.01.T01:CDS |
| GGGCAAAGCAGACAAGTGGT+TGG | 0.579650 | 5:+6109414 | None:intergenic |
| AGTCGTCTCAGATCATAGAA+TGG | 0.602234 | 5:-6108897 | MsG0580024533.01.T01:CDS |
| GCATTGATGATATCCTTGGA+TGG | 0.631536 | 5:-6108990 | MsG0580024533.01.T01:CDS |
| CAAAGGGCAAAGCAGACAAG+TGG | 0.655584 | 5:+6109410 | None:intergenic |
| GCTGCCCTTAGGAAGAAAAG+TGG | 0.661083 | 5:-6109482 | MsG0580024533.01.T01:CDS |
| CGTCTCAGATCATAGAATGG+CGG | 0.692691 | 5:-6108894 | MsG0580024533.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAGAGAAAATTACCAT+AGG | + | Chr5:6109038-6109057 | None:intergenic | 20.0% |
| !!! | TTGATCAACTTTTTAAATTC+AGG | + | Chr5:6109223-6109242 | None:intergenic | 20.0% |
| ! | AAAATCTTTCCACCAATTTA+GGG | + | Chr5:6109375-6109394 | None:intergenic | 25.0% |
| ! | ATTGTAGCTAAAAGTTTACT+CGG | - | Chr5:6109261-6109280 | MsG0580024533.01.T01:intron | 25.0% |
| ACAACTCATCAACTCAAATT+TGG | - | Chr5:6108953-6108972 | MsG0580024533.01.T01:CDS | 30.0% | |
| CAACTCATCAACTCAAATTT+GGG | - | Chr5:6108954-6108973 | MsG0580024533.01.T01:CDS | 30.0% | |
| GAAAATCTTTCCACCAATTT+AGG | + | Chr5:6109376-6109395 | None:intergenic | 30.0% | |
| TAAAGTCATTCTCCCTAAAT+TGG | - | Chr5:6109360-6109379 | MsG0580024533.01.T01:intron | 30.0% | |
| TAACTAACATTCACATTTCC+TGG | + | Chr5:6109084-6109103 | None:intergenic | 30.0% | |
| TTTGTAGTTTGAATATGACC+AGG | - | Chr5:6109063-6109082 | MsG0580024533.01.T01:CDS | 30.0% | |
| ! | TACCTGAATTTTGAACTGTA+AGG | + | Chr5:6109535-6109554 | None:intergenic | 30.0% |
| TGATATCCTTGGATGGATAA+TGG | - | Chr5:6109420-6109439 | MsG0580024533.01.T01:CDS | 35.0% | |
| ! | ATTCAGGTACATGTTTTCGA+AGG | - | Chr5:6109546-6109565 | MsG0580024533.01.T01:CDS | 35.0% |
| ! | CATTTTCCATTATCCATCCA+AGG | + | Chr5:6109429-6109448 | None:intergenic | 35.0% |
| ! | TGATGCATTGATGAATCACA+CGG | - | Chr5:6109151-6109170 | MsG0580024533.01.T01:intron | 35.0% |
| !! | ATCAGCATTGATGATATCCT+TGG | - | Chr5:6109409-6109428 | MsG0580024533.01.T01:CDS | 35.0% |
| !! | TTCACCACTTTTCTTCCTAA+GGG | + | Chr5:6108928-6108947 | None:intergenic | 35.0% |
| AGTCATTCTCCCTAAATTGG+TGG | - | Chr5:6109363-6109382 | MsG0580024533.01.T01:intron | 40.0% | |
| AGTCGTCTCAGATCATAGAA+TGG | - | Chr5:6109506-6109525 | MsG0580024533.01.T01:CDS | 40.0% | |
| GGCCTTACAGTTCAAAATTC+AGG | - | Chr5:6109530-6109549 | MsG0580024533.01.T01:CDS | 40.0% | |
| TGTCTGTAGAAACTCTCTCT+TGG | + | Chr5:6109317-6109336 | None:intergenic | 40.0% | |
| TTCGAATCTAAGAGAAGAGC+TGG | - | Chr5:6109285-6109304 | MsG0580024533.01.T01:intron | 40.0% | |
| ! | GCATTGATGATATCCTTGGA+TGG | - | Chr5:6109413-6109432 | MsG0580024533.01.T01:CDS | 40.0% |
| ! | TGCGTTTTTATCAAACGCAG+TGG | - | Chr5:6108880-6108899 | MsG0580024533.01.T01:CDS | 40.0% |
| !! | CTTCACCACTTTTCTTCCTA+AGG | + | Chr5:6108929-6108948 | None:intergenic | 40.0% |
| CGTCTCAGATCATAGAATGG+CGG | - | Chr5:6109509-6109528 | MsG0580024533.01.T01:CDS | 45.0% | |
| CAAAGGGCAAAGCAGACAAG+TGG | + | Chr5:6108996-6109015 | None:intergenic | 50.0% | |
| CATAGGATCCGACAATGCTC+CGG | + | Chr5:6109021-6109040 | None:intergenic | 50.0% | |
| GCTGCCCTTAGGAAGAAAAG+TGG | - | Chr5:6108921-6108940 | MsG0580024533.01.T01:CDS | 50.0% | |
| GGCAAAGCAGACAAGTGGTT+GGG | + | Chr5:6108991-6109010 | None:intergenic | 50.0% | |
| CGACAATGCTCCGGTGCAAA+GGG | + | Chr5:6109012-6109031 | None:intergenic | 55.0% | |
| GGGCAAAGCAGACAAGTGGT+TGG | + | Chr5:6108992-6109011 | None:intergenic | 55.0% | |
| GTCTGCTTTGCCCTTTGCAC+CGG | - | Chr5:6108999-6109018 | MsG0580024533.01.T01:CDS | 55.0% | |
| GTGTTGTGTCTGCTGCCCTT+AGG | - | Chr5:6108910-6108929 | MsG0580024533.01.T01:CDS | 55.0% | |
| !! | CCTTTGCACCGGAGCATTGT+CGG | - | Chr5:6109010-6109029 | MsG0580024533.01.T01:CDS | 55.0% |
| !! | CGGAGCATTGTCGGATCCTA+TGG | - | Chr5:6109019-6109038 | MsG0580024533.01.T01:CDS | 55.0% |
| CCGACAATGCTCCGGTGCAA+AGG | + | Chr5:6109013-6109032 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 6108837 | 6109588 | 6108837 | ID=MsG0580024533.01;Name=MsG0580024533.01 |
| Chr5 | mRNA | 6108837 | 6109588 | 6108837 | ID=MsG0580024533.01.T01;Parent=MsG0580024533.01;Name=MsG0580024533.01.T01;_AED=0.50;_eAED=0.54;_QI=0|0|0|1|1|1|2|0|174 |
| Chr5 | exon | 6109385 | 6109588 | 6109385 | ID=MsG0580024533.01.T01:exon:19269;Parent=MsG0580024533.01.T01 |
| Chr5 | exon | 6108837 | 6109157 | 6108837 | ID=MsG0580024533.01.T01:exon:19268;Parent=MsG0580024533.01.T01 |
| Chr5 | CDS | 6109385 | 6109588 | 6109385 | ID=MsG0580024533.01.T01:cds;Parent=MsG0580024533.01.T01 |
| Chr5 | CDS | 6108837 | 6109157 | 6108837 | ID=MsG0580024533.01.T01:cds;Parent=MsG0580024533.01.T01 |
| Gene Sequence |
| Protein sequence |
