AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580026179.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026179.01.T01 MTR_5g041040 90.336 238 13 1 1 238 1 228 5.58e-149 414
MsG0580026179.01.T01 MTR_5g041010 68.908 238 63 2 1 238 1 227 2.24e-120 342
MsG0580026179.01.T01 MTR_5g041080 71.130 239 58 2 1 238 1 229 1.61e-118 337
MsG0580026179.01.T01 MTR_5g041095 68.619 239 63 3 1 238 1 228 5.37e-106 305
MsG0580026179.01.T01 MTR_5g040940 57.009 214 82 1 25 238 25 228 1.31e-91 269
MsG0580026179.01.T01 MTR_5g040960 55.745 235 91 4 7 238 6 230 1.32e-89 264
MsG0580026179.01.T01 MTR_3g030640 35.176 199 115 9 43 236 79 268 1.74e-25 100
MsG0580026179.01.T01 MTR_3g030660 34.673 199 116 10 43 236 84 273 2.83e-24 97.8
MsG0580026179.01.T01 MTR_8g028080 36.139 202 95 7 26 204 41 231 9.13e-24 97.1
MsG0580026179.01.T01 MTR_5g086410 30.332 211 135 9 30 236 12 214 3.09e-21 88.6
MsG0580026179.01.T01 MTR_0129s0080 29.464 224 136 8 6 222 3 211 4.39e-21 88.6
MsG0580026179.01.T01 MTR_2g021750 33.708 178 107 7 46 222 165 332 1.26e-20 89.4
MsG0580026179.01.T01 MTR_6g071425 33.168 202 122 8 43 235 83 280 2.76e-20 87.4
MsG0580026179.01.T01 MTR_2g021830 32.597 181 105 8 46 224 14 179 1.72e-19 83.2
MsG0580026179.01.T01 MTR_5g086770 32.903 155 93 4 69 221 62 207 4.06e-18 80.5
MsG0580026179.01.T01 MTR_6g071505 33.005 203 117 8 43 235 76 269 1.71e-17 79.7
MsG0580026179.01.T01 MTR_2g104370 33.824 136 79 4 96 221 52 186 2.19e-17 77.8
MsG0580026179.01.T01 MTR_4g057825 28.910 211 120 10 28 236 21 203 1.03e-16 76.3
MsG0580026179.01.T01 MTR_6g090200 27.542 236 136 11 3 235 1 204 2.02e-16 75.5
MsG0580026179.01.T01 MTR_2g021910 28.037 214 128 9 21 232 534 723 2.65e-14 72.0
MsG0580026179.01.T01 MTR_2g021910 30.500 200 114 9 14 212 230 405 3.20e-14 72.0
MsG0580026179.01.T01 MTR_0002s1200 30.500 200 126 8 42 236 108 299 4.21e-14 70.5
MsG0580026179.01.T01 MTR_1g048200 28.141 199 114 9 43 222 109 297 1.35e-13 69.3
MsG0580026179.01.T01 MTR_5g022810 24.554 224 147 10 30 236 11 229 6.22e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026179.01.T01 AT1G26820 57.511 233 88 2 6 238 1 222 4.89e-101 293
MsG0580026179.01.T01 AT1G14220 55.794 233 92 2 6 238 7 228 2.74e-96 281
MsG0580026179.01.T01 AT2G02990 51.695 236 104 1 3 238 5 230 1.50e-89 264
MsG0580026179.01.T01 AT1G14210 45.690 232 111 4 9 236 6 226 6.73e-67 207
MsG0580026179.01.T01 AT2G39780 36.842 228 116 10 24 238 28 240 2.10e-33 121
MsG0580026179.01.T01 AT2G39780 30.837 227 99 10 24 238 28 208 1.98e-18 81.3

Find 47 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACATGTGTGCGGACATTTC+TGG 0.161608 5:+31857426 MsG0580026179.01.T01:CDS
TTAAGGACTTGGAAAATAAT+TGG 0.199378 5:+31856891 MsG0580026179.01.T01:CDS
GTGGAGTAAAATTTATTATC+TGG 0.272659 5:-31857306 None:intergenic
TCTCTATTACATTCTATTCC+TGG 0.336181 5:-31857372 None:intergenic
CTGAAAATGTATCTTCAATA+AGG 0.341118 5:+31857609 MsG0580026179.01.T01:three_prime_UTR
ATAGATCTCAGACCTTATTA+AGG 0.345632 5:+31856874 MsG0580026179.01.T01:intron
CTCAGACCTTATTAAGGACT+TGG 0.350106 5:+31856880 MsG0580026179.01.T01:CDS
AAGTGTCTTTGATAAATCTA+AGG 0.352998 5:+31856752 MsG0580026179.01.T01:CDS
TCTTGTAACCTGCATTCTTA+AGG 0.363598 5:-31857063 None:intergenic
ACATGTGTGCGGACATTTCT+GGG 0.368274 5:+31857427 MsG0580026179.01.T01:CDS
AAATAAATTTACTTAAAACT+TGG 0.373695 5:-31857511 None:intergenic
AATAAATTTACTTAAAACTT+GGG 0.399663 5:-31857510 None:intergenic
CTCTATTACATTCTATTCCT+GGG 0.404362 5:-31857371 None:intergenic
AACCTGCATTCTTAAGGATC+TGG 0.416497 5:-31857057 None:intergenic
AGTGAAATACCATTGCTACT+AGG 0.448454 5:-31856929 None:intergenic
GTGAAATACCATTGCTACTA+GGG 0.468797 5:-31856928 None:intergenic
CTCCAGATCCTTAAGAATGC+AGG 0.469885 5:+31857055 MsG0580026179.01.T01:CDS
GAGTGCCCTGGTCTTCCAAT+GGG 0.481203 5:+31857462 MsG0580026179.01.T01:CDS
AATCAAATTCTTGAGCAGAA+AGG 0.483669 5:-31856422 None:intergenic
TTTGAGTCACAGATTGCTCC+TGG 0.486696 5:-31856621 None:intergenic
ATACCCTGTAGCCTGTTGTA+TGG 0.491172 5:-31857346 None:intergenic
TTTCACTATGGTCACATGAA+TGG 0.494000 5:+31856945 MsG0580026179.01.T01:CDS
TGAGTGCCCTGGTCTTCCAA+TGG 0.494252 5:+31857461 MsG0580026179.01.T01:CDS
CAGCTACCCATTGGAAGACC+AGG 0.504798 5:-31857468 None:intergenic
CTTAGGCCTAACTTCAATGA+TGG 0.510786 5:+31856702 MsG0580026179.01.T01:CDS
CTACTAGGGCAGCTCAAAGA+TGG 0.516232 5:-31856914 None:intergenic
TTGCTAATGATGCAGTGGCC+AGG 0.521593 5:+31856603 MsG0580026179.01.T01:CDS
GATGATCCATCATTGAAGTT+AGG 0.526231 5:-31856708 None:intergenic
GTAGCAATGGTATTTCACTA+TGG 0.532633 5:+31856933 MsG0580026179.01.T01:CDS
TCAAAATTCATTGAGTGCCC+TGG 0.537116 5:+31857450 MsG0580026179.01.T01:CDS
ATATTGAAGTATCAAAAGCT+TGG 0.542439 5:-31856385 None:intergenic
ACATGTAAACTTGATAAAGC+TGG 0.553440 5:-31857410 None:intergenic
GATGCCATACAACAGGCTAC+AGG 0.561281 5:+31857342 MsG0580026179.01.T01:CDS
ATAAATTTACTTAAAACTTG+GGG 0.568252 5:-31857509 None:intergenic
TTGAGCTGCCCTAGTAGCAA+TGG 0.583698 5:+31856920 MsG0580026179.01.T01:CDS
TTAGCGCCACAGCTACCCAT+TGG 0.584200 5:-31857477 None:intergenic
GGTCTTCCAATGGGTAGCTG+TGG 0.598566 5:+31857471 MsG0580026179.01.T01:CDS
GGCATCTAAAATGTTACCTG+TGG 0.603734 5:-31857325 None:intergenic
TTATCAAGTTTACATGTGTG+CGG 0.617597 5:+31857416 MsG0580026179.01.T01:CDS
ATGCCATACAACAGGCTACA+GGG 0.622104 5:+31857343 MsG0580026179.01.T01:CDS
CAGGCTACAGGGTATTCCCC+AGG 0.622346 5:+31857354 MsG0580026179.01.T01:CDS
TCTATTACATTCTATTCCTG+GGG 0.626261 5:-31857370 None:intergenic
GTTAGGCCTAAGTCCAGCAA+TGG 0.644262 5:-31856691 None:intergenic
TTAATTTGCTAATGATGCAG+TGG 0.649424 5:+31856598 MsG0580026179.01.T01:CDS
GCAATGGTAAAATCTGCTGT+CGG 0.669210 5:-31856675 None:intergenic
AGCTACCCATTGGAAGACCA+GGG 0.682922 5:-31857467 None:intergenic
TCACATGAATGGATGAAACA+TGG 0.720853 5:+31856956 MsG0580026179.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAATTTACTTAAAACT+TGG - Chr5:31857514-31857533 None:intergenic 10.0%
!! AATAAATTTACTTAAAACTT+GGG - Chr5:31857513-31857532 None:intergenic 10.0%
!!! AATTAAATGATTAGAAATTT+TGG - Chr5:31857264-31857283 None:intergenic 10.0%
!!! TATTTTTATTATATAGAACA+TGG + Chr5:31857529-31857548 MsG0580026179.01.T01:three_prime_UTR 10.0%
!! ATAAATTTACTTAAAACTTG+GGG - Chr5:31857512-31857531 None:intergenic 15.0%
!! ATTTAATTGCATTGAATTTC+AGG + Chr5:31857276-31857295 MsG0580026179.01.T01:intron 20.0%
!! TTTAATTGCATTGAATTTCA+GGG + Chr5:31857277-31857296 MsG0580026179.01.T01:intron 20.0%
!!! ACTTTTAATATTACAGTTTG+AGG - Chr5:31856736-31856755 None:intergenic 20.0%
!!! CTTTTAATATTACAGTTTGA+GGG - Chr5:31856735-31856754 None:intergenic 20.0%
! AAGTGTCTTTGATAAATCTA+AGG + Chr5:31856752-31856771 MsG0580026179.01.T01:CDS 25.0%
! CTGAAAATGTATCTTCAATA+AGG + Chr5:31857609-31857628 MsG0580026179.01.T01:three_prime_UTR 25.0%
! GTGGAGTAAAATTTATTATC+TGG - Chr5:31857309-31857328 None:intergenic 25.0%
! GTTACATAAGTAATCATCAA+AGG - Chr5:31857102-31857121 None:intergenic 25.0%
! TTAAGGACTTGGAAAATAAT+TGG + Chr5:31856891-31856910 MsG0580026179.01.T01:CDS 25.0%
!! ATATTGAAGTATCAAAAGCT+TGG - Chr5:31856388-31856407 None:intergenic 25.0%
!! GATAATAAATTTTACTCCAC+AGG + Chr5:31857309-31857328 MsG0580026179.01.T01:CDS 25.0%
!! TATTTTCCAAGTCCTTAATA+AGG - Chr5:31856889-31856908 None:intergenic 25.0%
!! TTTCTTCTATTTTATTCTGC+AGG + Chr5:31856443-31856462 MsG0580026179.01.T01:CDS 25.0%
AAGCTTGGACAAAAATGAAA+TGG - Chr5:31856373-31856392 None:intergenic 30.0%
AATCAAATTCTTGAGCAGAA+AGG - Chr5:31856425-31856444 None:intergenic 30.0%
ACATGTAAACTTGATAAAGC+TGG - Chr5:31857413-31857432 None:intergenic 30.0%
ATAGATCTCAGACCTTATTA+AGG + Chr5:31856874-31856893 MsG0580026179.01.T01:intron 30.0%
CTCTATTACATTCTATTCCT+GGG - Chr5:31857374-31857393 None:intergenic 30.0%
TCTATTACATTCTATTCCTG+GGG - Chr5:31857373-31857392 None:intergenic 30.0%
TCTCTATTACATTCTATTCC+TGG - Chr5:31857375-31857394 None:intergenic 30.0%
TTATCAAGTTTACATGTGTG+CGG + Chr5:31857416-31857435 MsG0580026179.01.T01:CDS 30.0%
! TGTTTTCTCTTTCTCTTGAA+AGG - Chr5:31856330-31856349 None:intergenic 30.0%
! TTTCTCTTGAAAGGAGTTTT+TGG - Chr5:31856321-31856340 None:intergenic 30.0%
!! TTAATTTGCTAATGATGCAG+TGG + Chr5:31856598-31856617 MsG0580026179.01.T01:CDS 30.0%
AGTGAAATACCATTGCTACT+AGG - Chr5:31856932-31856951 None:intergenic 35.0%
GATGATCCATCATTGAAGTT+AGG - Chr5:31856711-31856730 None:intergenic 35.0%
GTAGCAATGGTATTTCACTA+TGG + Chr5:31856933-31856952 MsG0580026179.01.T01:CDS 35.0%
GTGAAATACCATTGCTACTA+GGG - Chr5:31856931-31856950 None:intergenic 35.0%
TCACATGAATGGATGAAACA+TGG + Chr5:31856956-31856975 MsG0580026179.01.T01:CDS 35.0%
TCTTGTAACCTGCATTCTTA+AGG - Chr5:31857066-31857085 None:intergenic 35.0%
TTTCACTATGGTCACATGAA+TGG + Chr5:31856945-31856964 MsG0580026179.01.T01:CDS 35.0%
! ATTTTACCATTGCTGGACTT+AGG + Chr5:31856685-31856704 MsG0580026179.01.T01:CDS 35.0%
! CATTTTAGATGCCATACAAC+AGG + Chr5:31857335-31857354 MsG0580026179.01.T01:CDS 35.0%
AACCTGCATTCTTAAGGATC+TGG - Chr5:31857060-31857079 None:intergenic 40.0%
CTCAGACCTTATTAAGGACT+TGG + Chr5:31856880-31856899 MsG0580026179.01.T01:CDS 40.0%
CTTAGGCCTAACTTCAATGA+TGG + Chr5:31856702-31856721 MsG0580026179.01.T01:CDS 40.0%
GCAATGGTAAAATCTGCTGT+CGG - Chr5:31856678-31856697 None:intergenic 40.0%
TCAAAATTCATTGAGTGCCC+TGG + Chr5:31857450-31857469 MsG0580026179.01.T01:CDS 40.0%
! ACAGCAGATTTTACCATTGC+TGG + Chr5:31856678-31856697 MsG0580026179.01.T01:CDS 40.0%
! GGCATCTAAAATGTTACCTG+TGG - Chr5:31857328-31857347 None:intergenic 40.0%
!!! AAAATCTGCTGTCGGTTTTC+CGG - Chr5:31856670-31856689 None:intergenic 40.0%
ACATGTGTGCGGACATTTCT+GGG + Chr5:31857427-31857446 MsG0580026179.01.T01:CDS 45.0%
ATACCCTGTAGCCTGTTGTA+TGG - Chr5:31857349-31857368 None:intergenic 45.0%
ATGCCATACAACAGGCTACA+GGG + Chr5:31857343-31857362 MsG0580026179.01.T01:CDS 45.0%
ATGTACAGCTCTCAACGCTT+TGG - Chr5:31857239-31857258 None:intergenic 45.0%
CTCCAGATCCTTAAGAATGC+AGG + Chr5:31857055-31857074 MsG0580026179.01.T01:CDS 45.0%
TACATGTGTGCGGACATTTC+TGG + Chr5:31857426-31857445 MsG0580026179.01.T01:CDS 45.0%
TGTGTCAGACGAGACAAGTA+TGG - Chr5:31857208-31857227 None:intergenic 45.0%
TTTGAGTCACAGATTGCTCC+TGG - Chr5:31856624-31856643 None:intergenic 45.0%
! TTTTCGCAAAACAGCAGCTC+TGG - Chr5:31856647-31856666 None:intergenic 45.0%
!!! AGCTGCTGTTTTGCGAAAAC+CGG + Chr5:31856648-31856667 MsG0580026179.01.T01:CDS 45.0%
AGCTACCCATTGGAAGACCA+GGG - Chr5:31857470-31857489 None:intergenic 50.0%
CTACTAGGGCAGCTCAAAGA+TGG - Chr5:31856917-31856936 None:intergenic 50.0%
GATGCCATACAACAGGCTAC+AGG + Chr5:31857342-31857361 MsG0580026179.01.T01:CDS 50.0%
GTTAGGCCTAAGTCCAGCAA+TGG - Chr5:31856694-31856713 None:intergenic 50.0%
TTGAGCTGCCCTAGTAGCAA+TGG + Chr5:31856920-31856939 MsG0580026179.01.T01:CDS 50.0%
TTGCTAATGATGCAGTGGCC+AGG + Chr5:31856603-31856622 MsG0580026179.01.T01:CDS 50.0%
CAGCTACCCATTGGAAGACC+AGG - Chr5:31857471-31857490 None:intergenic 55.0%
GGTCTTCCAATGGGTAGCTG+TGG + Chr5:31857471-31857490 MsG0580026179.01.T01:CDS 55.0%
GGTGTCTGACTGACACATCG+TGG - Chr5:31857187-31857206 None:intergenic 55.0%
TTAGCGCCACAGCTACCCAT+TGG - Chr5:31857480-31857499 None:intergenic 55.0%
! GAGTGCCCTGGTCTTCCAAT+GGG + Chr5:31857462-31857481 MsG0580026179.01.T01:CDS 55.0%
! TGAGTGCCCTGGTCTTCCAA+TGG + Chr5:31857461-31857480 MsG0580026179.01.T01:CDS 55.0%
! CAGGCTACAGGGTATTCCCC+AGG + Chr5:31857354-31857373 MsG0580026179.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 31856312 31857669 31856312 ID=MsG0580026179.01;Name=MsG0580026179.01
Chr5 mRNA 31856312 31857669 31856312 ID=MsG0580026179.01.T01;Parent=MsG0580026179.01;Name=MsG0580026179.01.T01;_AED=0.48;_eAED=0.48;_QI=45|0.66|0.75|1|1|1|4|148|238
Chr5 exon 31856312 31856464 31856312 ID=MsG0580026179.01.T01:exon:24956;Parent=MsG0580026179.01.T01
Chr5 exon 31856588 31856773 31856588 ID=MsG0580026179.01.T01:exon:24957;Parent=MsG0580026179.01.T01
Chr5 exon 31856878 31857076 31856878 ID=MsG0580026179.01.T01:exon:24958;Parent=MsG0580026179.01.T01
Chr5 exon 31857298 31857669 31857298 ID=MsG0580026179.01.T01:exon:24959;Parent=MsG0580026179.01.T01
Chr5 five_prime_UTR 31856312 31856356 31856312 ID=MsG0580026179.01.T01:five_prime_utr;Parent=MsG0580026179.01.T01
Chr5 CDS 31856357 31856464 31856357 ID=MsG0580026179.01.T01:cds;Parent=MsG0580026179.01.T01
Chr5 CDS 31856588 31856773 31856588 ID=MsG0580026179.01.T01:cds;Parent=MsG0580026179.01.T01
Chr5 CDS 31856878 31857076 31856878 ID=MsG0580026179.01.T01:cds;Parent=MsG0580026179.01.T01
Chr5 CDS 31857298 31857521 31857298 ID=MsG0580026179.01.T01:cds;Parent=MsG0580026179.01.T01
Chr5 three_prime_UTR 31857522 31857669 31857522 ID=MsG0580026179.01.T01:three_prime_utr;Parent=MsG0580026179.01.T01
Gene Sequence

>MsG0580026179.01.T01

ATGAAACTCAACACCATTTCATTTTTGTCCAAGCTTTTGATACTTCAATATCTTTCTGTTCAAAGCCTTTCTGCTCAAGAATTTGATTTCTTCTATTTTATTCTGCAGTATTGCACTGTTAATTTGCTAATGATGCAGTGGCCAGGAGCAATCTGTGACTCAAACCAGAGCTGCTGTTTTGCGAAAACCGGAAAACCGACAGCAGATTTTACCATTGCTGGACTTAGGCCTAACTTCAATGATGGATCATCTCCCTCAAACTGTAATATTAAAAGTGTCTTTGATAAATCTAAGATCTCAGACCTTATTAAGGACTTGGAAAATAATTGGCCATCTTTGAGCTGCCCTAGTAGCAATGGTATTTCACTATGGTCACATGAATGGATGAAACATGGTACATGTTCAGAATCTAAGCTTACTCAACATGACTACTTTCAAACAGCTCTCAAACTCAAGAAAAAATCAAATCTTCTCCAGATCCTTAAGAATGCAGGGATTGAACCAGATAATAAATTTTACTCCACAGGTAACATTTTAGATGCCATACAACAGGCTACAGGGTATTCCCCAGGAATAGAATGTAATAGAGATTCAGCTCATAACAGCCAGCTTTATCAAGTTTACATGTGTGCGGACATTTCTGGGTCAAAATTCATTGAGTGCCCTGGTCTTCCAATGGGTAGCTGTGGCGCTAATGTACAATTCCCCAAGTTTTAA

Protein sequence

>MsG0580026179.01.T01

MKLNTISFLSKLLILQYLSVQSLSAQEFDFFYFILQYCTVNLLMMQWPGAICDSNQSCCFAKTGKPTADFTIAGLRPNFNDGSSPSNCNIKSVFDKSKISDLIKDLENNWPSLSCPSSNGISLWSHEWMKHGTCSESKLTQHDYFQTALKLKKKSNLLQILKNAGIEPDNKFYSTGNILDAIQQATGYSPGIECNRDSAHNSQLYQVYMCADISGSKFIECPGLPMGSCGANVQFPKF*