AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580026027.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026027.01.T01 MTR_5g037930 98.438 128 2 0 1 128 1 128 1.24e-92 263
MsG0580026027.01.T01 MTR_5g037950 73.228 127 33 1 1 127 1 126 1.12e-54 167
MsG0580026027.01.T01 MTR_5g037890 68.750 128 38 1 1 128 1 126 6.31e-54 165
MsG0580026027.01.T01 MTR_7g009070 59.615 104 42 0 24 127 30 133 2.03e-44 142
MsG0580026027.01.T01 MTR_3g112410 47.664 107 56 0 20 126 6 112 8.67e-37 122
MsG0580026027.01.T01 MTR_1g023140 46.296 108 58 0 21 128 7 114 3.15e-36 120
MsG0580026027.01.T01 MTR_5g021180 42.478 113 65 0 14 126 2 114 9.52e-35 117
MsG0580026027.01.T01 MTR_8g107460 41.509 106 62 0 21 126 255 360 6.61e-28 105
MsG0580026027.01.T01 MTR_2g010750 38.095 105 65 0 20 124 9 113 2.11e-23 88.2
MsG0580026027.01.T01 MTR_7g073680 40.659 91 54 0 31 121 35 125 5.40e-22 85.1
MsG0580026027.01.T01 MTR_8g005320 35.238 105 68 0 19 123 25 129 8.15e-22 84.7
MsG0580026027.01.T01 MTR_8g006685 38.710 93 57 0 31 123 106 198 2.94e-21 85.1
MsG0580026027.01.T01 MTR_8g006560 38.710 93 57 0 31 123 106 198 2.94e-21 85.1
MsG0580026027.01.T01 MTR_2g082590 32.381 105 71 0 20 124 10 114 6.08e-21 82.0
MsG0580026027.01.T01 MTR_4g081380 37.273 110 67 2 20 128 10 118 1.41e-20 80.9
MsG0580026027.01.T01 MTR_2g082590 37.209 86 54 0 39 124 42 127 7.03e-19 77.0
MsG0580026027.01.T01 MTR_4g094690 36.585 82 50 1 46 125 85 166 2.33e-16 71.2
MsG0580026027.01.T01 MTR_1g098660 31.250 96 63 2 31 125 67 160 2.47e-16 71.2
MsG0580026027.01.T01 MTR_2g079420 37.500 88 53 2 40 125 84 171 2.38e-14 66.2
MsG0580026027.01.T01 MTR_8g059015 35.227 88 55 2 40 125 87 174 6.51e-14 65.1
MsG0580026027.01.T01 MTR_3g089065 32.143 84 55 2 45 126 89 172 3.15e-13 63.2
MsG0580026027.01.T01 MTR_7g080250 35.443 79 50 1 42 119 94 172 4.06e-13 63.2
MsG0580026027.01.T01 MTR_1g114290 32.911 79 49 2 40 117 84 159 5.73e-12 60.1
MsG0580026027.01.T01 MTR_4g085880 34.211 76 49 1 45 119 84 159 7.85e-12 59.3
MsG0580026027.01.T01 MTR_3g112400 30.928 97 47 3 20 116 5 81 2.95e-11 55.8
MsG0580026027.01.T01 MTR_2g079340 38.028 71 43 1 44 113 54 124 9.42e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026027.01.T01 AT5G39950 46.923 130 64 1 1 125 1 130 1.37e-42 137
MsG0580026027.01.T01 AT1G59730 42.969 128 72 1 1 127 1 128 4.29e-38 125
MsG0580026027.01.T01 AT1G69880 49.074 108 55 0 19 126 36 143 1.94e-35 119
MsG0580026027.01.T01 AT5G42980 47.222 108 57 0 21 128 6 113 1.47e-34 116
MsG0580026027.01.T01 AT1G19730 49.533 107 53 1 21 126 7 113 1.84e-34 116
MsG0580026027.01.T01 AT3G51030 44.340 106 59 0 21 126 7 112 6.30e-34 114
MsG0580026027.01.T01 AT1G45145 44.860 107 59 0 20 126 5 111 7.99e-34 114
MsG0580026027.01.T01 AT3G17880 40.000 105 63 0 22 126 265 369 3.24e-25 98.2
MsG0580026027.01.T01 AT3G17880 39.623 106 64 0 21 126 271 376 4.10e-25 98.2
MsG0580026027.01.T01 AT3G56420 39.130 92 56 0 30 121 50 141 1.40e-23 89.7
MsG0580026027.01.T01 AT3G56420 39.130 92 56 0 30 121 50 141 1.40e-23 89.7
MsG0580026027.01.T01 AT3G56420 39.130 92 56 0 30 121 50 141 1.40e-23 89.7
MsG0580026027.01.T01 AT1G11530 34.286 105 69 0 20 124 2 106 9.24e-21 81.3
MsG0580026027.01.T01 AT2G40790 39.130 92 56 0 30 121 50 141 1.22e-19 79.3
MsG0580026027.01.T01 AT2G40790 39.130 92 56 0 30 121 50 141 1.22e-19 79.3
MsG0580026027.01.T01 AT3G08710 34.951 103 65 1 21 123 26 126 1.27e-19 79.0
MsG0580026027.01.T01 AT3G08710 34.951 103 65 1 21 123 26 126 1.27e-19 79.0
MsG0580026027.01.T01 AT3G08710 34.951 103 65 1 21 123 26 126 1.27e-19 79.0
MsG0580026027.01.T01 AT3G15360 37.363 91 49 3 40 125 102 189 4.50e-16 71.2
MsG0580026027.01.T01 AT1G43560 30.159 126 79 5 9 128 39 161 9.80e-16 69.7
MsG0580026027.01.T01 AT2G35010 38.554 83 47 2 46 125 110 191 1.69e-14 67.0
MsG0580026027.01.T01 AT2G35010 38.554 83 47 2 46 125 110 191 1.69e-14 67.0
MsG0580026027.01.T01 AT1G76760 33.333 75 49 1 41 114 80 154 2.27e-14 66.2
MsG0580026027.01.T01 AT4G03520 34.940 83 52 2 45 125 101 183 8.46e-14 65.1
MsG0580026027.01.T01 AT1G31020 30.556 108 73 1 20 125 49 156 9.99e-14 64.3
MsG0580026027.01.T01 AT1G03680 35.802 81 50 2 47 125 97 177 2.42e-13 63.5
MsG0580026027.01.T01 AT3G02730 35.632 87 55 1 43 128 88 174 2.96e-13 63.5
MsG0580026027.01.T01 AT5G16400 34.091 88 57 1 42 128 97 184 4.07e-13 63.2
MsG0580026027.01.T01 AT1G52990 31.522 92 61 2 36 126 222 312 7.86e-12 61.2
MsG0580026027.01.T01 AT4G04950 29.787 94 66 0 32 125 11 104 2.59e-11 60.1
MsG0580026027.01.T01 AT4G32580 27.660 94 68 0 32 125 11 104 7.95e-11 56.6
MsG0580026027.01.T01 AT2G15570 25.843 89 65 1 32 119 74 162 8.91e-11 56.6
MsG0580026027.01.T01 AT2G15570 25.843 89 65 1 32 119 75 163 9.63e-11 56.6

Find 25 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCAGCTTGTTAGTTTCTT+TGG 0.210380 5:+29338101 None:intergenic
CCTTCCATTCCACTGCTAAA+TGG 0.240816 5:-29338143 MsG0580026027.01.T01:CDS
TAAGATGGTAATTGAATTTA+CGG 0.284078 5:-29337986 MsG0580026027.01.T01:intron
GATTGACGTGGATGAATTAA+TGG 0.293121 5:-29337881 MsG0580026027.01.T01:intron
GCAGCAAAGTCTTGAATGAT+TGG 0.331944 5:+29337931 None:intergenic
AAAGTTGTTGACAAGGTTGT+AGG 0.405303 5:-29337263 MsG0580026027.01.T01:CDS
CAGCTTGTTAGTTTCTTTGG+AGG 0.429788 5:+29338104 None:intergenic
TTCTTCATCAGTATGAATGT+TGG 0.495900 5:+29337290 None:intergenic
AAGTTGTTGACAAGGTTGTA+GGG 0.506438 5:-29337262 MsG0580026027.01.T01:CDS
CCTTCCATTTAGCAGTGGAA+TGG 0.530623 5:+29338139 None:intergenic
CTCCAAAGAAACTAACAAGC+TGG 0.546462 5:-29338103 MsG0580026027.01.T01:intron
GTGTGGACCTTGCAAATACA+TGG 0.552168 5:-29337956 MsG0580026027.01.T01:CDS
CCATTTAGCAGTGGAATGGA+AGG 0.552428 5:+29338143 None:intergenic
CCATTCCACTGCTAAATGGA+AGG 0.553763 5:-29338139 MsG0580026027.01.T01:CDS
ACATTCATACTGATGAAGAA+AGG 0.574346 5:-29337287 MsG0580026027.01.T01:CDS
ATTGGATCCATGTATTTGCA+AGG 0.586486 5:+29337949 None:intergenic
GGGAGGCAATTTATCCAACA+TGG 0.596510 5:-29338211 MsG0580026027.01.T01:CDS
GAATTTACGGCTGCATGGTG+TGG 0.600755 5:-29337973 MsG0580026027.01.T01:CDS
GAAAGGAAAAGTTGTTGACA+AGG 0.605147 5:-29337270 MsG0580026027.01.T01:CDS
AATTTATCCAACATGGAGCA+TGG 0.613422 5:-29338204 MsG0580026027.01.T01:CDS
ATAAGCCTTCCATTTAGCAG+TGG 0.613671 5:+29338134 None:intergenic
AGTTGTTGACAAGGTTGTAG+GGG 0.631631 5:-29337261 MsG0580026027.01.T01:CDS
TGTATGACCATGCTCCATGT+TGG 0.633501 5:+29338197 None:intergenic
AGATTTCATCAAGATTGACG+TGG 0.662014 5:-29337893 MsG0580026027.01.T01:CDS
TAATTGAATTTACGGCTGCA+TGG 0.665704 5:-29337978 MsG0580026027.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCTTTATATAATGTATAGA+TGG + Chr5:29337644-29337663 None:intergenic 15.0%
!!! AGATGAACTTTTTTTTTAAA+CGG - Chr5:29337981-29338000 MsG0580026027.01.T01:CDS 15.0%
!!! ATTATTTTTCAATTGTAAAC+AGG - Chr5:29338082-29338101 MsG0580026027.01.T01:intron 15.0%
!!! TATACTCAAATGATTATTTT+AGG + Chr5:29337808-29337827 None:intergenic 15.0%
!!! TTATTTTTCAATTGTAAACA+GGG - Chr5:29338083-29338102 MsG0580026027.01.T01:intron 15.0%
!! ATAATTAATGTATCAAACTC+CGG - Chr5:29337825-29337844 MsG0580026027.01.T01:intron 20.0%
!! TAAGATGGTAATTGAATTTA+CGG - Chr5:29337441-29337460 MsG0580026027.01.T01:intron 20.0%
!!! AATACTGTTAAGTTGAATTA+AGG + Chr5:29337861-29337880 None:intergenic 20.0%
!!! ATACTGTTAAGTTGAATTAA+GGG + Chr5:29337860-29337879 None:intergenic 20.0%
!!! GATGAACTTTTTTTTTAAAC+GGG - Chr5:29337982-29338001 MsG0580026027.01.T01:CDS 20.0%
!!! GTATTTTATGTGATTTACTA+TGG - Chr5:29337729-29337748 MsG0580026027.01.T01:intron 20.0%
!!! TTTGTTTTTTTAACTCTCAT+GGG - Chr5:29337399-29337418 MsG0580026027.01.T01:intron 20.0%
!!! TTTTGTTTTTTTAACTCTCA+TGG - Chr5:29337398-29337417 MsG0580026027.01.T01:intron 20.0%
! ATGCTATATCTCCTAATATA+AGG - Chr5:29337610-29337629 MsG0580026027.01.T01:intron 25.0%
! TGCTATATCTCCTAATATAA+GGG - Chr5:29337611-29337630 MsG0580026027.01.T01:intron 25.0%
!!! TGTATTTTTTTGGCTTAAGA+TGG - Chr5:29337426-29337445 MsG0580026027.01.T01:intron 25.0%
ACATTCATACTGATGAAGAA+AGG - Chr5:29338140-29338159 MsG0580026027.01.T01:CDS 30.0%
GAATTAAGGGATATAAAAGC+CGG + Chr5:29337847-29337866 None:intergenic 30.0%
GTTATGACTAGACACAAAAT+TGG - Chr5:29338006-29338025 MsG0580026027.01.T01:intron 30.0%
TTCTTCATCAGTATGAATGT+TGG + Chr5:29338140-29338159 None:intergenic 30.0%
!! ATTTGAAACGAGACTTTTTG+AGG - Chr5:29338044-29338063 MsG0580026027.01.T01:intron 30.0%
AAAGTTGTTGACAAGGTTGT+AGG - Chr5:29338164-29338183 MsG0580026027.01.T01:CDS 35.0%
AAGTTGTTGACAAGGTTGTA+GGG - Chr5:29338165-29338184 MsG0580026027.01.T01:CDS 35.0%
AATTTATCCAACATGGAGCA+TGG - Chr5:29337223-29337242 MsG0580026027.01.T01:CDS 35.0%
AGATTTCATCAAGATTGACG+TGG - Chr5:29337534-29337553 MsG0580026027.01.T01:intron 35.0%
ATTGGATCCATGTATTTGCA+AGG + Chr5:29337481-29337500 None:intergenic 35.0%
GAAAGGAAAAGTTGTTGACA+AGG - Chr5:29338157-29338176 MsG0580026027.01.T01:CDS 35.0%
GATTGACGTGGATGAATTAA+TGG - Chr5:29337546-29337565 MsG0580026027.01.T01:intron 35.0%
TAATTGAATTTACGGCTGCA+TGG - Chr5:29337449-29337468 MsG0580026027.01.T01:intron 35.0%
! TGGTCTTTTTCCATACAAAC+AGG - Chr5:29337749-29337768 MsG0580026027.01.T01:intron 35.0%
!!! CATGGGATCGTGTATTTTTT+TGG - Chr5:29337416-29337435 MsG0580026027.01.T01:intron 35.0%
AGTTGTTGACAAGGTTGTAG+GGG - Chr5:29338166-29338185 MsG0580026027.01.T01:CDS 40.0%
ATAAGCCTTCCATTTAGCAG+TGG + Chr5:29337296-29337315 None:intergenic 40.0%
CCATACAAACAGGATGACTA+AGG - Chr5:29337759-29337778 MsG0580026027.01.T01:intron 40.0%
CCTTAGTCATCCTGTTTGTA+TGG + Chr5:29337762-29337781 None:intergenic 40.0%
CTCCAAAGAAACTAACAAGC+TGG - Chr5:29337324-29337343 MsG0580026027.01.T01:CDS 40.0%
GCAGCAAAGTCTTGAATGAT+TGG + Chr5:29337499-29337518 None:intergenic 40.0%
!! CACCAGCTTGTTAGTTTCTT+TGG + Chr5:29337329-29337348 None:intergenic 40.0%
!! CAGCTTGTTAGTTTCTTTGG+AGG + Chr5:29337326-29337345 None:intergenic 40.0%
CAAGGTTGTAGGGGCAAAAA+AGG - Chr5:29338175-29338194 MsG0580026027.01.T01:CDS 45.0%
CCATTCCACTGCTAAATGGA+AGG - Chr5:29337288-29337307 MsG0580026027.01.T01:CDS 45.0%
CCATTTAGCAGTGGAATGGA+AGG + Chr5:29337287-29337306 None:intergenic 45.0%
CCTTCCATTCCACTGCTAAA+TGG - Chr5:29337284-29337303 MsG0580026027.01.T01:CDS 45.0%
CCTTCCATTTAGCAGTGGAA+TGG + Chr5:29337291-29337310 None:intergenic 45.0%
GGGAGGCAATTTATCCAACA+TGG - Chr5:29337216-29337235 MsG0580026027.01.T01:CDS 45.0%
GTGTGGACCTTGCAAATACA+TGG - Chr5:29337471-29337490 MsG0580026027.01.T01:intron 45.0%
TGGTGTGCTGCCCTTATATT+AGG + Chr5:29337624-29337643 None:intergenic 45.0%
TGTATGACCATGCTCCATGT+TGG + Chr5:29337233-29337252 None:intergenic 45.0%
GAATTTACGGCTGCATGGTG+TGG - Chr5:29337454-29337473 MsG0580026027.01.T01:intron 50.0%
Chromosome Type Strat End Strand Name
Chr5 gene 29337214 29338235 29337214 ID=MsG0580026027.01;Name=MsG0580026027.01
Chr5 mRNA 29337214 29338235 29337214 ID=MsG0580026027.01.T01;Parent=MsG0580026027.01;Name=MsG0580026027.01.T01;_AED=0.36;_eAED=0.36;_QI=0|0|0|1|1|1|3|0|128
Chr5 exon 29338104 29338235 29338104 ID=MsG0580026027.01.T01:exon:33477;Parent=MsG0580026027.01.T01
Chr5 exon 29337882 29338004 29337882 ID=MsG0580026027.01.T01:exon:33476;Parent=MsG0580026027.01.T01
Chr5 exon 29337214 29337345 29337214 ID=MsG0580026027.01.T01:exon:33475;Parent=MsG0580026027.01.T01
Chr5 CDS 29338104 29338235 29338104 ID=MsG0580026027.01.T01:cds;Parent=MsG0580026027.01.T01
Chr5 CDS 29337882 29338004 29337882 ID=MsG0580026027.01.T01:cds;Parent=MsG0580026027.01.T01
Chr5 CDS 29337214 29337345 29337214 ID=MsG0580026027.01.T01:cds;Parent=MsG0580026027.01.T01
Gene Sequence

>MsG0580026027.01.T01

ATGGGAGGCAATTTATCCAACATGGAGCATGGTCATACATCAGCAAAGTCATTATCATCTCACATTCTTACCTTCCATTCCACTGCTAAATGGAAGGCTTATTTTGATGCCTCCAAAGAAACTAACAAGCTGATGGTAATTGAATTTACGGCTGCATGGTGTGGACCTTGCAAATACATGGATCCAATCATTCAAGACTTTGCTGCAAAATACATAAAAGTAGATTTCATCAAGATTGACGTGGATGAATTAATGGGTGTCTCTCAAGAGTTTCAAGTGCAAGCAATGCCAACATTCATACTGATGAAGAAAGGAAAAGTTGTTGACAAGGTTGTAGGGGCAAAAAAGGAAGAGCTTGAAAAGTTGATTGAGAAGCATCAAAACTAA

Protein sequence

>MsG0580026027.01.T01

MGGNLSNMEHGHTSAKSLSSHILTFHSTAKWKAYFDASKETNKLMVIEFTAAWCGPCKYMDPIIQDFAAKYIKVDFIKIDVDELMGVSQEFQVQAMPTFILMKKGKVVDKVVGAKKEELEKLIEKHQN*