AlfalfaGEDB | MsG0580026027.01

M. sativa cultivar ZhongmuNo.1 / MsG0580026027.01

KEGG: K03671
GO: GO:0006662 GO:0015035 GO:0045454
PFAM: PF00085

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026027.01.T01	XP_003613548.1	98.438	128	2	0	1	128	1	128	1.03E-88	263

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026027.01.T01	Q38879	46.923	130	64	1	1	125	1	130	1.35E-41	137

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080049008.01	MsG0580026027.01	0.808794	2.599418e-50	1.500121e-47
MsG0180006182.01	MsG0580026027.01	0.813889	2.036925e-51	1.346582e-48
MsG0180006191.01	MsG0580026027.01	0.840787	7.043329e-58	1.014221e-54
MsG0280007427.01	MsG0580026027.01	0.841003	6.182029e-58	8.960685e-55
MsG0380013041.01	MsG0580026027.01	0.826183	3.120336e-54	2.905168e-51
MsG0380016372.01	MsG0580026027.01	0.800514	1.390862e-48	6.488679e-46
MsG0380016485.01	MsG0580026027.01	0.827548	1.472179e-54	1.425746e-51
MsG0380017898.01	MsG0580026027.01	0.822472	2.326497e-53	1.949179e-50
MsG0480022407.01	MsG0580026027.01	0.802786	4.756108e-49	2.350601e-46
MsG0480023322.01	MsG0580026027.01	0.810470	1.134584e-50	6.844954e-48

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PPI

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Gene1	Gene2	Type
MsG0580026027.01	MsG0280006562.01	PPI
MsG0580026027.01	MsG0280006562.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026027.01.T01	MTR_5g037930	98.438	128	2	0	1	128	1	128	1.24e-92	263
MsG0580026027.01.T01	MTR_5g037950	73.228	127	33	1	1	127	1	126	1.12e-54	167
MsG0580026027.01.T01	MTR_5g037890	68.750	128	38	1	1	128	1	126	6.31e-54	165
MsG0580026027.01.T01	MTR_7g009070	59.615	104	42	0	24	127	30	133	2.03e-44	142
MsG0580026027.01.T01	MTR_3g112410	47.664	107	56	0	20	126	6	112	8.67e-37	122
MsG0580026027.01.T01	MTR_1g023140	46.296	108	58	0	21	128	7	114	3.15e-36	120
MsG0580026027.01.T01	MTR_5g021180	42.478	113	65	0	14	126	2	114	9.52e-35	117
MsG0580026027.01.T01	MTR_8g107460	41.509	106	62	0	21	126	255	360	6.61e-28	105
MsG0580026027.01.T01	MTR_2g010750	38.095	105	65	0	20	124	9	113	2.11e-23	88.2
MsG0580026027.01.T01	MTR_7g073680	40.659	91	54	0	31	121	35	125	5.40e-22	85.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026027.01.T01	AT5G39950	46.923	130	64	1	1	125	1	130	1.37e-42	137
MsG0580026027.01.T01	AT1G59730	42.969	128	72	1	1	127	1	128	4.29e-38	125
MsG0580026027.01.T01	AT1G69880	49.074	108	55	0	19	126	36	143	1.94e-35	119
MsG0580026027.01.T01	AT5G42980	47.222	108	57	0	21	128	6	113	1.47e-34	116
MsG0580026027.01.T01	AT1G19730	49.533	107	53	1	21	126	7	113	1.84e-34	116
MsG0580026027.01.T01	AT3G51030	44.340	106	59	0	21	126	7	112	6.30e-34	114
MsG0580026027.01.T01	AT1G45145	44.860	107	59	0	20	126	5	111	7.99e-34	114
MsG0580026027.01.T01	AT3G17880	40.000	105	63	0	22	126	265	369	3.24e-25	98.2
MsG0580026027.01.T01	AT3G17880	39.623	106	64	0	21	126	271	376	4.10e-25	98.2
MsG0580026027.01.T01	AT3G56420	39.130	92	56	0	30	121	50	141	1.40e-23	89.7

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Find 25 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTTGTTGACAAGGTTGT+AGG	0.405303	5:-29337263	MsG0580026027.01.T01:CDS
AAGTTGTTGACAAGGTTGTA+GGG	0.506438	5:-29337262	MsG0580026027.01.T01:CDS
AATTTATCCAACATGGAGCA+TGG	0.613422	5:-29338204	MsG0580026027.01.T01:CDS
ACATTCATACTGATGAAGAA+AGG	0.574346	5:-29337287	MsG0580026027.01.T01:CDS
AGATTTCATCAAGATTGACG+TGG	0.662014	5:-29337893	MsG0580026027.01.T01:CDS
AGTTGTTGACAAGGTTGTAG+GGG	0.631631	5:-29337261	MsG0580026027.01.T01:CDS
ATAAGCCTTCCATTTAGCAG+TGG	0.613671	5:+29338134	None:intergenic
ATTGGATCCATGTATTTGCA+AGG	0.586486	5:+29337949	None:intergenic
CACCAGCTTGTTAGTTTCTT+TGG	0.210380	5:+29338101	None:intergenic
CAGCTTGTTAGTTTCTTTGG+AGG	0.429788	5:+29338104	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACATTCATACTGATGAAGAA+AGG	-	Chr5:29338140-29338159	MsG0580026027.01.T01:CDS	30.0%
GAATTAAGGGATATAAAAGC+CGG	+	Chr5:29337847-29337866	None:intergenic	30.0%
GTTATGACTAGACACAAAAT+TGG	-	Chr5:29338006-29338025	MsG0580026027.01.T01:intron	30.0%
TTCTTCATCAGTATGAATGT+TGG	+	Chr5:29338140-29338159	None:intergenic	30.0%
AAAGTTGTTGACAAGGTTGT+AGG	-	Chr5:29338164-29338183	MsG0580026027.01.T01:CDS	35.0%
AAGTTGTTGACAAGGTTGTA+GGG	-	Chr5:29338165-29338184	MsG0580026027.01.T01:CDS	35.0%
AATTTATCCAACATGGAGCA+TGG	-	Chr5:29337223-29337242	MsG0580026027.01.T01:CDS	35.0%
AGATTTCATCAAGATTGACG+TGG	-	Chr5:29337534-29337553	MsG0580026027.01.T01:intron	35.0%
ATTGGATCCATGTATTTGCA+AGG	+	Chr5:29337481-29337500	None:intergenic	35.0%
GAAAGGAAAAGTTGTTGACA+AGG	-	Chr5:29338157-29338176	MsG0580026027.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	29337214	29338235	29337214	ID=MsG0580026027.01;Name=MsG0580026027.01
Chr5	mRNA	29337214	29338235	29337214	ID=MsG0580026027.01.T01;Parent=MsG0580026027.01;Name=MsG0580026027.01.T01;_AED=0.36;_eAED=0.36;_QI=0\|0\|0\|1\|1\|1\|3\|0\|128
Chr5	exon	29338104	29338235	29338104	ID=MsG0580026027.01.T01:exon:33477;Parent=MsG0580026027.01.T01
Chr5	exon	29337882	29338004	29337882	ID=MsG0580026027.01.T01:exon:33476;Parent=MsG0580026027.01.T01
Chr5	exon	29337214	29337345	29337214	ID=MsG0580026027.01.T01:exon:33475;Parent=MsG0580026027.01.T01
Chr5	CDS	29338104	29338235	29338104	ID=MsG0580026027.01.T01:cds;Parent=MsG0580026027.01.T01
Chr5	CDS	29337882	29338004	29337882	ID=MsG0580026027.01.T01:cds;Parent=MsG0580026027.01.T01
Chr5	CDS	29337214	29337345	29337214	ID=MsG0580026027.01.T01:cds;Parent=MsG0580026027.01.T01

Gene Sequence

>MsG0580026027.01.T01

ATGGGAGGCAATTTATCCAACATGGAGCATGGTCATACATCAGCAAAGTCATTATCATCTCACATTCTTACCTTCCATTCCACTGCTAAATGGAAGGCTTATTTTGATGCCTCCAAAGAAACTAACAAGCTGATGGTAATTGAATTTACGGCTGCATGGTGTGGACCTTGCAAATACATGGATCCAATCATTCAAGACTTTGCTGCAAAATACATAAAAGTAGATTTCATCAAGATTGACGTGGATGAATTAATGGGTGTCTCTCAAGAGTTTCAAGTGCAAGCAATGCCAACATTCATACTGATGAAGAAAGGAAAAGTTGTTGACAAGGTTGTAGGGGCAAAAAAGGAAGAGCTTGAAAAGTTGATTGAGAAGCATCAAAACTAA

Protein sequence

>MsG0580026027.01.T01

MGGNLSNMEHGHTSAKSLSSHILTFHSTAKWKAYFDASKETNKLMVIEFTAAWCGPCKYMDPIIQDFAAKYIKVDFIKIDVDELMGVSQEFQVQAMPTFILMKKGKVVDKVVGAKKEELEKLIEKHQN*