Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025620.01.T01 | XP_003613016.1 | 96.839 | 601 | 13 | 2 | 1 | 600 | 1 | 596 | 0 | 1181 |
| MsG0580025620.01.T02 | XP_003613016.1 | 96.839 | 601 | 13 | 2 | 1 | 600 | 1 | 596 | 0 | 1181 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047967.01 | MsG0580025620.01 | -0.847907 | 8.592621e-60 | 1.550205e-56 |
| MsG0080048694.01 | MsG0580025620.01 | -0.883027 | 6.349035e-71 | 3.958517e-67 |
| MsG0080048695.01 | MsG0580025620.01 | -0.882552 | 9.465999e-71 | 5.790946e-67 |
| MsG0080048697.01 | MsG0580025620.01 | -0.807631 | 4.598698e-50 | 2.574301e-47 |
| MsG0080048889.01 | MsG0580025620.01 | 0.860785 | 1.621588e-63 | 4.486024e-60 |
| MsG0080048919.01 | MsG0580025620.01 | -0.825961 | 3.523532e-54 | 3.259913e-51 |
| MsG0080048948.01 | MsG0580025620.01 | -0.821172 | 4.649689e-53 | 3.755006e-50 |
| MsG0080049008.01 | MsG0580025620.01 | 0.816302 | 5.931641e-52 | 4.187667e-49 |
| MsG0080049015.01 | MsG0580025620.01 | -0.809041 | 2.301631e-50 | 1.337087e-47 |
| MsG0080049019.01 | MsG0580025620.01 | -0.857239 | 1.868584e-62 | 4.581244e-59 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025620.01.T01 | MTR_5g031720 | 96.839 | 601 | 13 | 2 | 1 | 600 | 1 | 596 | 0.0 | 1181 |
| MsG0580025620.01.T01 | MTR_5g049770 | 80.000 | 215 | 38 | 3 | 380 | 592 | 28 | 239 | 9.17e-111 | 332 |
| MsG0580025620.01.T01 | MTR_5g049770 | 69.863 | 73 | 22 | 0 | 145 | 217 | 26 | 98 | 1.19e-25 | 106 |
| MsG0580025620.01.T01 | MTR_4g093650 | 34.861 | 611 | 333 | 15 | 37 | 594 | 13 | 611 | 3.90e-94 | 301 |
| MsG0580025620.01.T01 | MTR_2g009650 | 35.537 | 484 | 254 | 12 | 18 | 446 | 18 | 498 | 2.35e-71 | 244 |
| MsG0580025620.01.T01 | MTR_2g009650 | 30.387 | 362 | 190 | 15 | 276 | 594 | 419 | 761 | 6.29e-20 | 94.7 |
| MsG0580025620.01.T01 | MTR_3g110650 | 29.455 | 679 | 344 | 22 | 28 | 600 | 12 | 661 | 1.53e-52 | 191 |
| MsG0580025620.01.T01 | MTR_2g019090 | 25.705 | 603 | 380 | 13 | 44 | 594 | 42 | 628 | 1.30e-46 | 174 |
| MsG0580025620.01.T01 | MTR_4g008080 | 32.874 | 435 | 233 | 13 | 27 | 449 | 1 | 388 | 5.56e-45 | 169 |
| MsG0580025620.01.T01 | MTR_4g008080 | 28.947 | 228 | 139 | 7 | 8 | 227 | 186 | 398 | 7.66e-18 | 87.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025620.01.T01 | AT2G22600 | 34.748 | 636 | 325 | 13 | 31 | 594 | 12 | 629 | 4.35e-103 | 325 |
| MsG0580025620.01.T01 | AT1G51580 | 31.062 | 631 | 332 | 17 | 44 | 599 | 19 | 621 | 4.15e-67 | 230 |
| MsG0580025620.01.T01 | AT1G51580 | 33.846 | 195 | 109 | 6 | 16 | 204 | 248 | 428 | 1.44e-19 | 93.2 |
| MsG0580025620.01.T01 | AT5G46190 | 27.083 | 624 | 360 | 17 | 44 | 596 | 44 | 643 | 5.43e-48 | 178 |
| MsG0580025620.01.T01 | AT5G46190 | 27.485 | 171 | 108 | 3 | 43 | 210 | 316 | 473 | 1.53e-11 | 67.8 |
| MsG0580025620.01.T01 | AT5G46190 | 27.083 | 624 | 360 | 17 | 44 | 596 | 44 | 643 | 5.43e-48 | 178 |
| MsG0580025620.01.T01 | AT5G46190 | 27.485 | 171 | 108 | 3 | 43 | 210 | 316 | 473 | 1.53e-11 | 67.8 |
| MsG0580025620.01.T01 | AT5G46190 | 27.083 | 624 | 360 | 17 | 44 | 596 | 44 | 643 | 5.43e-48 | 178 |
| MsG0580025620.01.T01 | AT5G46190 | 27.485 | 171 | 108 | 3 | 43 | 210 | 316 | 473 | 1.53e-11 | 67.8 |
| MsG0580025620.01.T01 | AT5G53060 | 28.595 | 598 | 363 | 17 | 46 | 598 | 70 | 648 | 6.65e-47 | 175 |
Find 33 sgRNAs with CRISPR-Local
Find 529 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACTGCACAAGCAAGCTGA+TGG | 0.546668 | 5:-21375953 | MsG0580025620.01.T01:exon |
| AACCTTCCCTCCACTTGTAC+CGG | 0.534868 | 5:+21376037 | None:intergenic |
| AATGTGATAGTACCAATAAA+CGG | 0.506544 | 5:+21375668 | None:intergenic |
| AATGTGTGTTAGTCTACAGG+AGG | 0.692029 | 5:+21375761 | None:intergenic |
| ACAGGAGGAGGTGCCATTAG+TGG | 0.480446 | 5:-21375882 | MsG0580025620.01.T01:three_prime_UTR |
| ACATTTGAGACTGAATTAAT+TGG | 0.262244 | 5:-21375650 | MsG0580025620.01.T01:intron |
| AGCAGCACCGGTACAAGTGG+AGG | 0.530406 | 5:-21376044 | MsG0580025620.01.T01:CDS |
| AGTACCAATAAACGGTATAA+CGG | 0.506573 | 5:+21375676 | None:intergenic |
| AGTTCTTCCTTCAAAACAGG+AGG | 0.563223 | 5:-21375897 | MsG0580025620.01.T01:three_prime_UTR |
| ATATGTACACCAACCACTAA+TGG | 0.431634 | 5:+21375869 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGGATTCATTCATACTAAG+TGG | - | Chr5:21375221-21375240 | MsG0580025620.01.T01:intron | 30.0% | |
| AATCAAAAGTTTGAACCCTT+TGG | - | Chr5:21374799-21374818 | MsG0580025620.01.T01:intron | 30.0% | |
| AATCTTGTACTAAAAACCCT+TGG | + | Chr5:21377731-21377750 | None:intergenic | 30.0% | |
| AATTACTTGATTCAAGGTTC+TGG | + | Chr5:21376096-21376115 | None:intergenic | 30.0% | |
| ACAAGATACATAATTGTACC+TGG | + | Chr5:21376369-21376388 | None:intergenic | 30.0% | |
| AGCTATTGTATCCAACATAA+AGG | - | Chr5:21376920-21376939 | MsG0580025620.01.T01:intron | 30.0% | |
| AGTACCAATAAACGGTATAA+CGG | + | Chr5:21379171-21379190 | None:intergenic | 30.0% | |
| ATAACCAATCTCCTTTCTTA+AGG | + | Chr5:21375151-21375170 | None:intergenic | 30.0% | |
| ATAGATAAACCTTCCATTGA+AGG | + | Chr5:21375758-21375777 | None:intergenic | 30.0% | |
| ATCAAAAGTTTGAACCCTTT+GGG | - | Chr5:21374800-21374819 | MsG0580025620.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 21373474 | 21381392 | 21373474 | ID=MsG0580025620.01;Name=MsG0580025620.01 |
| Chr5 | mRNA | 21373474 | 21381392 | 21373474 | ID=MsG0580025620.01.T01;Parent=MsG0580025620.01;Name=MsG0580025620.01.T01;_AED=0.46;_eAED=0.46;_QI=154|0.85|0.75|1|0.85|0.75|8|301|600 |
| Chr5 | exon | 21373474 | 21373499 | 21373474 | ID=MsG0580025620.01.T01:exon:9115;Parent=MsG0580025620.01.T01 |
| Chr5 | exon | 21375681 | 21376099 | 21375681 | ID=MsG0580025620.01.T01:exon:9114;Parent=MsG0580025620.01.T01 |
| Chr5 | five_prime_UTR | 21381239 | 21381392 | 21381239 | ID=MsG0580025620.01.T01:five_prime_utr;Parent=MsG0580025620.01.T01 |
| Chr5 | CDS | 21380672 | 21381238 | 21380672 | ID=MsG0580025620.01.T01:cds;Parent=MsG0580025620.01.T01 |
| Chr5 | CDS | 21378854 | 21379285 | 21378854 | ID=MsG0580025620.01.T01:cds;Parent=MsG0580025620.01.T01 |
| Chr5 | CDS | 21378497 | 21378769 | 21378497 | ID=MsG0580025620.01.T01:cds;Parent=MsG0580025620.01.T01 |
| Chr5 | CDS | 21377198 | 21377389 | 21377198 | ID=MsG0580025620.01.T01:cds;Parent=MsG0580025620.01.T01 |
| Chr5 | CDS | 21377009 | 21377091 | 21377009 | ID=MsG0580025620.01.T01:cds;Parent=MsG0580025620.01.T01 |
| Chr5 | CDS | 21376662 | 21376773 | 21376662 | ID=MsG0580025620.01.T01:cds;Parent=MsG0580025620.01.T01 |
| Chr5 | CDS | 21375956 | 21376099 | 21375956 | ID=MsG0580025620.01.T01:cds;Parent=MsG0580025620.01.T01 |
| Chr5 | three_prime_UTR | 21375681 | 21375955 | 21375681 | ID=MsG0580025620.01.T01:three_prime_utr;Parent=MsG0580025620.01.T01 |
| Chr5 | three_prime_UTR | 21373474 | 21373499 | 21373474 | ID=MsG0580025620.01.T01:three_prime_utr;Parent=MsG0580025620.01.T01 |
| Chr5 | mRNA | 21375641 | 21381392 | 21375641 | ID=MsG0580025620.01.T02;Parent=MsG0580025620.01;Name=MsG0580025620.01.T02;_AED=0.46;_eAED=0.46;_QI=154|0.83|0.71|1|1|1|7|315|600 |
| Chr5 | exon | 21375641 | 21376099 | 21375641 | ID=MsG0580025620.01.T02:exon:9116;Parent=MsG0580025620.01.T02 |
| Chr5 | five_prime_UTR | 21381239 | 21381392 | 21381239 | ID=MsG0580025620.01.T02:five_prime_utr;Parent=MsG0580025620.01.T02 |
| Chr5 | CDS | 21380672 | 21381238 | 21380672 | ID=MsG0580025620.01.T02:cds;Parent=MsG0580025620.01.T02 |
| Chr5 | CDS | 21378854 | 21379285 | 21378854 | ID=MsG0580025620.01.T02:cds;Parent=MsG0580025620.01.T02 |
| Chr5 | CDS | 21378497 | 21378769 | 21378497 | ID=MsG0580025620.01.T02:cds;Parent=MsG0580025620.01.T02 |
| Chr5 | CDS | 21377198 | 21377389 | 21377198 | ID=MsG0580025620.01.T02:cds;Parent=MsG0580025620.01.T02 |
| Chr5 | CDS | 21377009 | 21377091 | 21377009 | ID=MsG0580025620.01.T02:cds;Parent=MsG0580025620.01.T02 |
| Chr5 | CDS | 21376662 | 21376773 | 21376662 | ID=MsG0580025620.01.T02:cds;Parent=MsG0580025620.01.T02 |
| Chr5 | CDS | 21375956 | 21376099 | 21375956 | ID=MsG0580025620.01.T02:cds;Parent=MsG0580025620.01.T02 |
| Chr5 | three_prime_UTR | 21375641 | 21375955 | 21375641 | ID=MsG0580025620.01.T02:three_prime_utr;Parent=MsG0580025620.01.T02 |
| Gene Sequence |
>MsG0580025620.01.T01 ATGCAAGACCCCACTTTCTCCGCCGCCGTTGAAACCACTGATCACCACCACCCACAACCACCACCACCACCGTCGTCGTTGAAACGGAACAACCGAAAACGACCCGTTTTCAAACTTCTACCGGGTCAGATCGCGTTTCGTTTGGTCTGTCATTCGTCAACGGTTGGTGGGTTGATCGGAAGTTCAGGGTCAATCGTTTCTCAGCTCCGGCGAGAAACCGGATGCAAAATCCACTGCGAGGATGCGGTGGTTGGGACTGAAGATAGGGTTATTTTGGTAATTGGGTCGATGTCGGATCGAAAAGGGATTGTTTTGGGTGATAGAGAAATGGAGGTTTCTAATGCACAAGAAGGTGTTGTTAGGGTTTTTGATAGGGTTTGGGGGCTTGAAAGTGAGAAAGGGGGAAATGGTGAAGTTTATGGGAAATTGTTGGCACATAGTTCTCAAATTGGTGCTATTGTTGGGAAAGGAGGGAAGAATATAAGTAATATTAGGAATAATAGTGGTTCGAATATTAGGGTTTGTCCTGCTCCACATTGTGCTGCTAAAGACGAGGAGTTAATTCTTATAACTGGTGAAAGTTTGGCTGTAAAGAAAGCACTGATTTATGTATCTTATTGTCTTCAAGATTGTCCACCGATGGGCAAAGTATCTTTGAACACGCCACCAACTATTAACTCGTCTGATAGGTCAACCTCTTACACACATGAGGATCTTTTTCCTCACCTTAATTCATGGTTACCTTCAATGGAAGGTTTATCTATAAACGATGCTTCAAAACAGACAACAAACGCTAACGGAAACTCTAGCATTGACTCAAAAGGTTCCGGACACGCAGTTGTTTTCAGACTACTTTGCTCTAACAACGTAGCTGGTAGTGTGATCGGCAAAAAAGGGTCTATAGTTAGAACTTTTGAAATCCAAACTGGTGCTTCTATTGTCTTTGCCCCTCCTTTAGGTCAGTATGAAGAGCGCATCGTCACTATTTCTGCTTTTGAGAACCTTGAATCAAGTAATTCTCCTGCACAAGATGCTGTTATTCTTGTCTTCACTAGAATTGCTGAAGATCACATTCGAAATGGATTTCAGCCAGCTACTGCTGTGGAATCACCTGTAACTGCGAGGCTTTTAATCACAACAAGCACGTTACATTTATTGACTGGTAATGAAGGTCAGGTGATTTCAGAATTGAGAGAAGTGAGTCGGGCTGATATTCAACTACTGCATGGAGAGCCAATTCCTAATGCTTCAGATAATGATGTGGTAGTCCAGATCACTGGTGGATACAGATGCGTAGAAAATGCTTTACGTAAAATCACTTCTATAATTAGGGATAATCCTTTGCCCAATGAGGTGCTTGCTGAAGCAAGAATCAAACCAAGTTTTCCTTTAAATAAAGATACTGTTAGGAGTAAGTTCACTACCCGCAAAAAACCTTTTCCTTTTGCGAGGTTTCTACCACAGAATGCAGGGGTGTATCAAGCTAAAAAGGTCACAGAAAATGGGGAATCACATACGAATTTAATCGAGAATGTGGAACCTGGCAGAGGAAACATTGTTGCCACTGTGACAAACACTACCGTGGAGATTATAGTCTCGGAGCATGTCTTTGGTTCTGTTTATGGTGAAGATGGCGGCAATTTAGATCGAATTAGACAGATTTCAGGTGCAGATGTTACTGTATTTGATCCCAGCAGCACCGGTACAAGTGGAGGGAAGGTTGTGATATCTGGAACACCTGATCAAACATTTGCAGCTCAGAGTTTGCTTCAAGCTTTCATTCAAACTGCACAAGCAAGCTGA >MsG0580025620.01.T02 ATGCAAGACCCCACTTTCTCCGCCGCCGTTGAAACCACTGATCACCACCACCCACAACCACCACCACCACCGTCGTCGTTGAAACGGAACAACCGAAAACGACCCGTTTTCAAACTTCTACCGGGTCAGATCGCGTTTCGTTTGGTCTGTCATTCGTCAACGGTTGGTGGGTTGATCGGAAGTTCAGGGTCAATCGTTTCTCAGCTCCGGCGAGAAACCGGATGCAAAATCCACTGCGAGGATGCGGTGGTTGGGACTGAAGATAGGGTTATTTTGGTAATTGGGTCGATGTCGGATCGAAAAGGGATTGTTTTGGGTGATAGAGAAATGGAGGTTTCTAATGCACAAGAAGGTGTTGTTAGGGTTTTTGATAGGGTTTGGGGGCTTGAAAGTGAGAAAGGGGGAAATGGTGAAGTTTATGGGAAATTGTTGGCACATAGTTCTCAAATTGGTGCTATTGTTGGGAAAGGAGGGAAGAATATAAGTAATATTAGGAATAATAGTGGTTCGAATATTAGGGTTTGTCCTGCTCCACATTGTGCTGCTAAAGACGAGGAGTTAATTCTTATAACTGGTGAAAGTTTGGCTGTAAAGAAAGCACTGATTTATGTATCTTATTGTCTTCAAGATTGTCCACCGATGGGCAAAGTATCTTTGAACACGCCACCAACTATTAACTCGTCTGATAGGTCAACCTCTTACACACATGAGGATCTTTTTCCTCACCTTAATTCATGGTTACCTTCAATGGAAGGTTTATCTATAAACGATGCTTCAAAACAGACAACAAACGCTAACGGAAACTCTAGCATTGACTCAAAAGGTTCCGGACACGCAGTTGTTTTCAGACTACTTTGCTCTAACAACGTAGCTGGTAGTGTGATCGGCAAAAAAGGGTCTATAGTTAGAACTTTTGAAATCCAAACTGGTGCTTCTATTGTCTTTGCCCCTCCTTTAGGTCAGTATGAAGAGCGCATCGTCACTATTTCTGCTTTTGAGAACCTTGAATCAAGTAATTCTCCTGCACAAGATGCTGTTATTCTTGTCTTCACTAGAATTGCTGAAGATCACATTCGAAATGGATTTCAGCCAGCTACTGCTGTGGAATCACCTGTAACTGCGAGGCTTTTAATCACAACAAGCACGTTACATTTATTGACTGGTAATGAAGGTCAGGTGATTTCAGAATTGAGAGAAGTGAGTCGGGCTGATATTCAACTACTGCATGGAGAGCCAATTCCTAATGCTTCAGATAATGATGTGGTAGTCCAGATCACTGGTGGATACAGATGCGTAGAAAATGCTTTACGTAAAATCACTTCTATAATTAGGGATAATCCTTTGCCCAATGAGGTGCTTGCTGAAGCAAGAATCAAACCAAGTTTTCCTTTAAATAAAGATACTGTTAGGAGTAAGTTCACTACCCGCAAAAAACCTTTTCCTTTTGCGAGGTTTCTACCACAGAATGCAGGGGTGTATCAAGCTAAAAAGGTCACAGAAAATGGGGAATCACATACGAATTTAATCGAGAATGTGGAACCTGGCAGAGGAAACATTGTTGCCACTGTGACAAACACTACCGTGGAGATTATAGTCTCGGAGCATGTCTTTGGTTCTGTTTATGGTGAAGATGGCGGCAATTTAGATCGAATTAGACAGATTTCAGGTGCAGATGTTACTGTATTTGATCCCAGCAGCACCGGTACAAGTGGAGGGAAGGTTGTGATATCTGGAACACCTGATCAAACATTTGCAGCTCAGAGTTTGCTTCAAGCTTTCATTCAAACTGCACAAGCAAGCTGA |
| Protein sequence |
>MsG0580025620.01.T01 MQDPTFSAAVETTDHHHPQPPPPPSSLKRNNRKRPVFKLLPGQIAFRLVCHSSTVGGLIGSSGSIVSQLRRETGCKIHCEDAVVGTEDRVILVIGSMSDRKGIVLGDREMEVSNAQEGVVRVFDRVWGLESEKGGNGEVYGKLLAHSSQIGAIVGKGGKNISNIRNNSGSNIRVCPAPHCAAKDEELILITGESLAVKKALIYVSYCLQDCPPMGKVSLNTPPTINSSDRSTSYTHEDLFPHLNSWLPSMEGLSINDASKQTTNANGNSSIDSKGSGHAVVFRLLCSNNVAGSVIGKKGSIVRTFEIQTGASIVFAPPLGQYEERIVTISAFENLESSNSPAQDAVILVFTRIAEDHIRNGFQPATAVESPVTARLLITTSTLHLLTGNEGQVISELREVSRADIQLLHGEPIPNASDNDVVVQITGGYRCVENALRKITSIIRDNPLPNEVLAEARIKPSFPLNKDTVRSKFTTRKKPFPFARFLPQNAGVYQAKKVTENGESHTNLIENVEPGRGNIVATVTNTTVEIIVSEHVFGSVYGEDGGNLDRIRQISGADVTVFDPSSTGTSGGKVVISGTPDQTFAAQSLLQAFIQTAQAS* >MsG0580025620.01.T02 MQDPTFSAAVETTDHHHPQPPPPPSSLKRNNRKRPVFKLLPGQIAFRLVCHSSTVGGLIGSSGSIVSQLRRETGCKIHCEDAVVGTEDRVILVIGSMSDRKGIVLGDREMEVSNAQEGVVRVFDRVWGLESEKGGNGEVYGKLLAHSSQIGAIVGKGGKNISNIRNNSGSNIRVCPAPHCAAKDEELILITGESLAVKKALIYVSYCLQDCPPMGKVSLNTPPTINSSDRSTSYTHEDLFPHLNSWLPSMEGLSINDASKQTTNANGNSSIDSKGSGHAVVFRLLCSNNVAGSVIGKKGSIVRTFEIQTGASIVFAPPLGQYEERIVTISAFENLESSNSPAQDAVILVFTRIAEDHIRNGFQPATAVESPVTARLLITTSTLHLLTGNEGQVISELREVSRADIQLLHGEPIPNASDNDVVVQITGGYRCVENALRKITSIIRDNPLPNEVLAEARIKPSFPLNKDTVRSKFTTRKKPFPFARFLPQNAGVYQAKKVTENGESHTNLIENVEPGRGNIVATVTNTTVEIIVSEHVFGSVYGEDGGNLDRIRQISGADVTVFDPSSTGTSGGKVVISGTPDQTFAAQSLLQAFIQTAQAS* |
