AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022407.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022407.01.T01 MTR_4g088980 97.059 306 8 1 1 305 1 306 0.0 606
MsG0480022407.01.T01 MTR_2g013220 68.439 301 83 5 9 305 21 313 3.28e-122 353
MsG0480022407.01.T01 MTR_8g098515 51.084 323 119 8 14 305 32 346 5.63e-73 229
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022407.01.T01 AT2G20760 52.303 304 119 8 21 301 22 322 1.15e-71 225
MsG0480022407.01.T01 AT2G40060 55.300 217 86 4 28 237 16 228 1.36e-65 207
MsG0480022407.01.T01 AT3G51890 61.635 159 58 1 87 245 85 240 5.46e-58 187

Find 112 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATCCTTTGGTTGTGATTC+AGG 0.112857 4:+72165811 None:intergenic
GTTTGCCTGGCTTTGGGCCT+TGG 0.150519 4:+72166013 None:intergenic
CGGAGGAAGGTAACGTTCTT+AGG 0.187267 4:-72168335 MsG0480022407.01.T01:CDS
CGGTGGGTTTGCCTGGCTTT+GGG 0.248540 4:+72166007 None:intergenic
GCAAACCCACCGACCTTTCT+AGG 0.257216 4:-72165996 MsG0480022407.01.T01:CDS
TCGGTGGGTTTGCCTGGCTT+TGG 0.260717 4:+72166006 None:intergenic
CCGAATACATCCGGAGATTC+CGG 0.326239 4:+72168501 None:intergenic
TTTGTGAAAATTCTCTTGAT+TGG 0.330165 4:+72166152 None:intergenic
GGCGACGACGACAACTATTC+TGG 0.330828 4:-72168585 MsG0480022407.01.T01:CDS
TGAATCTACAGTACCGTTAT+TGG 0.331003 4:+72165786 None:intergenic
GGTGCTGATCCATTTGGTTT+AGG 0.333375 4:+72165844 None:intergenic
CCAGTGCATGTGATGGAAAA+TGG 0.335341 4:-72168435 MsG0480022407.01.T01:CDS
CTTCCTCCTCCTGCTGAGAT+GGG 0.344842 4:-72168360 MsG0480022407.01.T01:CDS
GCTTCCTCCTCCTGCTGAGA+TGG 0.352439 4:-72168361 MsG0480022407.01.T01:CDS
TGAGAAGAAAAGAGGCAAAA+AGG 0.365407 4:-72166067 MsG0480022407.01.T01:CDS
AAAATTACTGGAAAGCAATT+GGG 0.366531 4:-72166119 MsG0480022407.01.T01:CDS
TGCCTGGCTTTGGGCCTTGG+AGG 0.380338 4:+72166016 None:intergenic
TTCCCTTTGGAGTCTTTAGC+AGG 0.387984 4:+72165907 None:intergenic
CCGTTATTGGCAGCATCCTT+TGG 0.394987 4:+72165799 None:intergenic
GGTTTAGGTGCTGATCCATT+TGG 0.396493 4:+72165859 None:intergenic
GAAAGGTCGGTGGGTTTGCC+TGG 0.402706 4:+72166000 None:intergenic
CCGGAATCTCCGGATGTATT+CGG 0.406444 4:-72168501 MsG0480022407.01.T01:CDS
GCTGCTGTTGTGTGATCTAC+AGG 0.409676 4:+72168525 None:intergenic
ATTATTGTCTATTATGACCT+TGG 0.418043 4:-72165573 MsG0480022407.01.T01:three_prime_UTR
ACTTCCGGTGCTGATCCATT+TGG 0.420411 4:+72165838 None:intergenic
AACTAGACGAGAAGGAGAAA+AGG 0.420773 4:-72166397 MsG0480022407.01.T01:CDS
AACAGCAGCGCCGGAATCTC+CGG 0.421407 4:-72168511 MsG0480022407.01.T01:CDS
TTGGAGTCTTTAGCAGGTGC+AGG 0.422981 4:+72165913 None:intergenic
TGTGATTCAGGTGCAACTTC+CGG 0.424313 4:+72165823 None:intergenic
CTCAATGTTGGCAACCTCAC+GGG 0.426203 4:+72166086 None:intergenic
GCAGACTGCTCCTCAGTGGC+AGG 0.431231 4:+72165748 None:intergenic
ACAAGGTTCAAAACAGAGAA+AGG 0.447399 4:-72166286 MsG0480022407.01.T01:CDS
TCCTCCGGCCCCATCTCAGC+AGG 0.449365 4:+72168351 None:intergenic
GTTTGTGGAACAGTTATACT+TGG 0.453267 4:-72166175 MsG0480022407.01.T01:intron
TCCTCCTGCTGAGATGGGGC+CGG 0.456220 4:-72168355 MsG0480022407.01.T01:CDS
GGTGAAGTGGTCTTCGTTGA+CGG 0.458970 4:+72168611 None:intergenic
AATGAGATTAAAGATTATTG+AGG 0.464614 4:-72166366 MsG0480022407.01.T01:CDS
ATGGAAAATGGAAACGGTTA+TGG 0.472080 4:-72168423 MsG0480022407.01.T01:CDS
GAAACAGCAACTAAACCAAA+TGG 0.475248 4:-72165874 MsG0480022407.01.T01:CDS
AAGGTCATAATAGACAATAA+TGG 0.479197 4:+72165575 None:intergenic
AGAATAGTTGTCGTCGTCGC+CGG 0.485975 4:+72168587 None:intergenic
AGGAGGAAGCACCGGACCAT+CGG 0.488490 4:+72168374 None:intergenic
GGATCAGCACCTAAACCAAA+TGG 0.488856 4:-72165853 MsG0480022407.01.T01:CDS
CATCACATGCACTGGTTCGA+AGG 0.488969 4:+72168443 None:intergenic
GTGATCTACAGGGACATCTT+CGG 0.492594 4:+72168536 None:intergenic
AAGTGGGTTTCTATGAGAAA+AGG 0.493819 4:-72166328 MsG0480022407.01.T01:CDS
GTGGCAGGAGCTTCTTTCTG+AGG 0.503664 4:+72165763 None:intergenic
ACTAGACGAGAAGGAGAAAA+GGG 0.504571 4:-72166396 MsG0480022407.01.T01:CDS
ATGTAACATTTGAATTTCCA+AGG 0.506294 4:+72165556 None:intergenic
GGAGGAAGGTAACGTTCTTA+GGG 0.507710 4:-72168334 MsG0480022407.01.T01:CDS
GTCCTCCAAGGCCCAAAGCC+AGG 0.509150 4:-72166018 MsG0480022407.01.T01:CDS
TTAGCAGGTGCAGGTGGAGG+AGG 0.510354 4:+72165922 None:intergenic
GGCCCCATCTCAGCAGGAGG+AGG 0.510809 4:+72168357 None:intergenic
CCAAAGGATGCTGCCAATAA+CGG 0.510873 4:-72165799 MsG0480022407.01.T01:CDS
ATATCTGCCGCATCCTAGAA+AGG 0.513686 4:+72165983 None:intergenic
TCCTGCTGAGATGGGGCCGG+AGG 0.515292 4:-72168352 MsG0480022407.01.T01:CDS
TGTATTCGGATTCAGCGATC+AGG 0.515580 4:-72168487 MsG0480022407.01.T01:CDS
CGTCGTCGCCGGCGGTGAAG+TGG 0.522107 4:+72168598 None:intergenic
TCTTTAGCAGGTGCAGGTGG+AGG 0.524857 4:+72165919 None:intergenic
GACGACAACTATTCTGGATA+CGG 0.532542 4:-72168579 MsG0480022407.01.T01:CDS
CAAGGTTCAAAACAGAGAAA+GGG 0.537446 4:-72166285 MsG0480022407.01.T01:CDS
CTAAGAACGTTACCTTCCTC+CGG 0.537633 4:+72168336 None:intergenic
TTGGAAATTCAAATGTTACA+TGG 0.538799 4:-72165554 MsG0480022407.01.T01:three_prime_UTR
TCTCAATGTTGGCAACCTCA+CGG 0.539692 4:+72166085 None:intergenic
AAGGGAGACTGAGCGTAACT+CGG 0.540887 4:+72168462 None:intergenic
AAGGTAACGTTCTTAGGGAA+TGG 0.547512 4:-72168329 MsG0480022407.01.T01:CDS
CACCTGCTAAAGACTCCAAA+GGG 0.547927 4:-72165909 MsG0480022407.01.T01:CDS
AACGAAGACCACTTCACCGC+CGG 0.548831 4:-72168606 MsG0480022407.01.T01:CDS
GAGTCTTTAGCAGGTGCAGG+TGG 0.551285 4:+72165916 None:intergenic
TCGCTGAATCCGAATACATC+CGG 0.554031 4:+72168492 None:intergenic
TGCTATTCAACTAGACGAGA+AGG 0.554504 4:-72166405 MsG0480022407.01.T01:CDS
TCAAAACAGAGAAAGGGAAA+AGG 0.555817 4:-72166279 MsG0480022407.01.T01:intron
CTTCGAACCAGTGCATGTGA+TGG 0.556019 4:-72168442 MsG0480022407.01.T01:CDS
GCTGAGATGGGGCCGGAGGA+AGG 0.557921 4:-72168348 MsG0480022407.01.T01:CDS
GTGGTCTTCGTTGACGGTGA+AGG 0.559914 4:+72168617 None:intergenic
GCCAACATTGAGAAGAAAAG+AGG 0.561154 4:-72166075 MsG0480022407.01.T01:CDS
AAAGATTATTGAGGAAGCTG+AGG 0.562392 4:-72166357 MsG0480022407.01.T01:CDS
TCCGGCCCCATCTCAGCAGG+AGG 0.568004 4:+72168354 None:intergenic
GAAGCTGAGGATTACAAAGT+GGG 0.570600 4:-72166344 MsG0480022407.01.T01:CDS
TTCGTTGACGGTGAAGGACA+TGG 0.571845 4:+72168623 None:intergenic
ATCACATGCACTGGTTCGAA+GGG 0.575990 4:+72168444 None:intergenic
AGATCACACAACAGCAGCGC+CGG 0.576259 4:-72168520 MsG0480022407.01.T01:CDS
TCAGCAGGAGGAGGAAGCAC+CGG 0.587615 4:+72168366 None:intergenic
TTCCTCCTCCTGCTGAGATG+GGG 0.587840 4:-72168359 MsG0480022407.01.T01:CDS
AAATTACTGGAAAGCAATTG+GGG 0.589625 4:-72166118 MsG0480022407.01.T01:CDS
CCTTGGAGGACAGTAACTGA+TGG 0.599991 4:+72166030 None:intergenic
CCACCGACCTTTCTAGGATG+CGG 0.602155 4:-72165990 MsG0480022407.01.T01:CDS
TGCACCTGAATCACAACCAA+AGG 0.604455 4:-72165815 MsG0480022407.01.T01:CDS
ATAGTTGTCGTCGTCGCCGG+CGG 0.604799 4:+72168590 None:intergenic
GGAGGAGGAATCATGTGAAG+TGG 0.606489 4:+72165937 None:intergenic
TGCTAAAGACTCCAAAGGGA+AGG 0.606563 4:-72165905 MsG0480022407.01.T01:CDS
CTGCCGCATCCTAGAAAGGT+CGG 0.609710 4:+72165987 None:intergenic
GGAAGCTGAGGATTACAAAG+TGG 0.610216 4:-72166345 MsG0480022407.01.T01:CDS
CGCATCCTAGAAAGGTCGGT+GGG 0.610689 4:+72165991 None:intergenic
TCAATGTTGGCAACCTCACG+GGG 0.611587 4:+72166087 None:intergenic
CCATCAGTTACTGTCCTCCA+AGG 0.615544 4:-72166030 MsG0480022407.01.T01:CDS
CCGCATCCTAGAAAGGTCGG+TGG 0.616621 4:+72165990 None:intergenic
TAAACCAAATGGATCAGCAC+CGG 0.618396 4:-72165842 MsG0480022407.01.T01:CDS
GAAAATGGAAACGGTTATGG+TGG 0.626405 4:-72168420 MsG0480022407.01.T01:CDS
AAAGACTCCAAAGGGAAGGA+CGG 0.629704 4:-72165901 MsG0480022407.01.T01:CDS
GCACCTGCTAAAGACTCCAA+AGG 0.630783 4:-72165910 MsG0480022407.01.T01:CDS
CTGCTGTTGTGTGATCTACA+GGG 0.636206 4:+72168526 None:intergenic
CATGTGATGGAAAATGGAAA+CGG 0.640946 4:-72168429 MsG0480022407.01.T01:CDS
GACAACTATTCTGGATACGG+CGG 0.653119 4:-72168576 MsG0480022407.01.T01:CDS
ACGTTCTTAGGGAATGGCGC+CGG 0.656812 4:-72168323 MsG0480022407.01.T01:intron
ATCTACAGGGACATCTTCGG+CGG 0.664893 4:+72168539 None:intergenic
TTAATGATTGAAATAAAATG+CGG 0.669839 4:+72165617 None:intergenic
GAAAGAAGCTCCTGCCACTG+AGG 0.676516 4:-72165758 MsG0480022407.01.T01:CDS
TGCAGCAGACTGCTCCTCAG+TGG 0.680257 4:+72165744 None:intergenic
GGAGGAATCATGTGAAGTGG+TGG 0.685796 4:+72165940 None:intergenic
TGGGGAGATAATTCCCCGTG+AGG 0.688947 4:-72166100 MsG0480022407.01.T01:CDS
GCTCAATGTTGAGACAAACA+AGG 0.723788 4:-72166303 MsG0480022407.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAATAGTATTTGTATTAA+TGG + Chr4:72167639-72167658 None:intergenic 15.0%
!! TAATAATTAAAAGTAGAGTA+AGG - Chr4:72167473-72167492 MsG0480022407.01.T01:intron 15.0%
!! TAATACAAATACTATTTGTT+TGG - Chr4:72167640-72167659 MsG0480022407.01.T01:intron 15.0%
!! TATAGTAAAAAATATAAGGA+AGG - Chr4:72166554-72166573 MsG0480022407.01.T01:intron 15.0%
!! TTAATGATTGAAATAAAATG+CGG + Chr4:72168592-72168611 None:intergenic 15.0%
!!! ATCTCTAAAAATTAAAAGAA+CGG + Chr4:72168004-72168023 None:intergenic 15.0%
!! AAAAAAAAGGTTAAAAATGC+AGG + Chr4:72168539-72168558 None:intergenic 20.0%
!! AAAAAAAGGTTAAAAATGCA+GGG + Chr4:72168538-72168557 None:intergenic 20.0%
!! AACATTTATTGGATTTAATG+AGG - Chr4:72166461-72166480 MsG0480022407.01.T01:intron 20.0%
!! AATGAGATTAAAGATTATTG+AGG - Chr4:72167840-72167859 MsG0480022407.01.T01:intron 20.0%
!! ACATTTATTGGATTTAATGA+GGG - Chr4:72166462-72166481 MsG0480022407.01.T01:intron 20.0%
!! AGATGAAATTAAGATATGTT+TGG - Chr4:72167171-72167190 MsG0480022407.01.T01:intron 20.0%
!! ATTGTCTTGAAAACATTTAT+TGG - Chr4:72166450-72166469 MsG0480022407.01.T01:intron 20.0%
!! CCATCATATTATTAAATGAT+GGG - Chr4:72167109-72167128 MsG0480022407.01.T01:intron 20.0%
!! TAAAAAATATAAGGAAGGAA+GGG - Chr4:72166559-72166578 MsG0480022407.01.T01:intron 20.0%
!! TAAAAAGTAAGTTCAGAATA+AGG - Chr4:72167302-72167321 MsG0480022407.01.T01:intron 20.0%
!! TCCATCATATTATTAAATGA+TGG - Chr4:72167108-72167127 MsG0480022407.01.T01:intron 20.0%
!! TTTATGTTAATTGTCTATGT+TGG - Chr4:72166649-72166668 MsG0480022407.01.T01:intron 20.0%
!!! GCATTTTATTTCAATCATTA+AGG - Chr4:72168591-72168610 MsG0480022407.01.T01:CDS 20.0%
!!! GTAAAAAGTAATGATAATGT+AGG + Chr4:72167966-72167985 None:intergenic 20.0%
!!! TAATTTTTTTCCTTCGAATT+TGG - Chr4:72166828-72166847 MsG0480022407.01.T01:intron 20.0%
!!! TTATATCCATCGATTAAAAA+AGG - Chr4:72166901-72166920 MsG0480022407.01.T01:intron 20.0%
! AAAAAAGGTTAAAAATGCAG+GGG + Chr4:72168537-72168556 None:intergenic 25.0%
! AAAAATTACTGGAAAGCAAT+TGG - Chr4:72168086-72168105 MsG0480022407.01.T01:intron 25.0%
! AAAATTACTGGAAAGCAATT+GGG - Chr4:72168087-72168106 MsG0480022407.01.T01:intron 25.0%
! AAAGTTTAAACTACCTAAGA+TGG + Chr4:72167393-72167412 None:intergenic 25.0%
! AAATACTTGTCCTACATTTA+AGG + Chr4:72167691-72167710 None:intergenic 25.0%
! AAATGGTTGATCTTTAAACA+TGG - Chr4:72167531-72167550 MsG0480022407.01.T01:intron 25.0%
! AACTAGAAGTCTAGAAATAA+AGG + Chr4:72166809-72166828 None:intergenic 25.0%
! AAGGTCATAATAGACAATAA+TGG + Chr4:72168634-72168653 None:intergenic 25.0%
! ACTAGAAGTCTAGAAATAAA+GGG + Chr4:72166808-72166827 None:intergenic 25.0%
! ATGTAACATTTGAATTTCCA+AGG + Chr4:72168653-72168672 None:intergenic 25.0%
! ATTATTGTCTATTATGACCT+TGG - Chr4:72168633-72168652 MsG0480022407.01.T01:exon 25.0%
! CCCATCATTTAATAATATGA+TGG + Chr4:72167112-72167131 None:intergenic 25.0%
! GTAAAAAATATAAGGAAGGA+AGG - Chr4:72166558-72166577 MsG0480022407.01.T01:intron 25.0%
! TTATTCTGAACCTTAAATGT+AGG - Chr4:72167678-72167697 MsG0480022407.01.T01:intron 25.0%
! TTGGAAATTCAAATGTTACA+TGG - Chr4:72168652-72168671 MsG0480022407.01.T01:five_prime_UTR 25.0%
! TTTGTGAAAATTCTCTTGAT+TGG + Chr4:72168057-72168076 None:intergenic 25.0%
!! AATTTGGTTTCTTTATGTGA+TGG - Chr4:72166844-72166863 MsG0480022407.01.T01:intron 25.0%
!! ACTAAAAGCATGCAAAAAAA+AGG + Chr4:72168552-72168571 None:intergenic 25.0%
!! ATGTTTTCTTTTGAATGCTT+AGG - Chr4:72166016-72166035 MsG0480022407.01.T01:CDS 25.0%
!! GATCTTAAATCTCTAAAGAT+CGG + Chr4:72167723-72167742 None:intergenic 25.0%
!! TTATTTTCGTAGGAAAAGTA+GGG - Chr4:72166876-72166895 MsG0480022407.01.T01:intron 25.0%
!!! ATTCGTAAAAGCATCAATAA+CGG - Chr4:72167429-72167448 MsG0480022407.01.T01:intron 25.0%
!!! TTTATTTTCGTAGGAAAAGT+AGG - Chr4:72166875-72166894 MsG0480022407.01.T01:intron 25.0%
!!! TTTTTTTCTTCTTCCATCTT+AGG - Chr4:72167377-72167396 MsG0480022407.01.T01:intron 25.0%
AAACACGATATGCATATGTT+TGG + Chr4:72166631-72166650 None:intergenic 30.0%
AAATTACTGGAAAGCAATTG+GGG - Chr4:72168088-72168107 MsG0480022407.01.T01:intron 30.0%
AATATGTCTGTGGATCAATT+GGG - Chr4:72166318-72166337 MsG0480022407.01.T01:CDS 30.0%
AATGCTTAGGATTATGAATC+AGG - Chr4:72166029-72166048 MsG0480022407.01.T01:CDS 30.0%
ACTATTTGTTTGGTCATATG+AGG - Chr4:72167650-72167669 MsG0480022407.01.T01:intron 30.0%
ATGCTTAGGATTATGAATCA+GGG - Chr4:72166030-72166049 MsG0480022407.01.T01:CDS 30.0%
CATAAAGAAACCAAATTCGA+AGG + Chr4:72166841-72166860 None:intergenic 30.0%
CCATCAAAAAAACTTGGTTT+CGG + Chr4:72166132-72166151 None:intergenic 30.0%
CTAGATCTAAGTTTAACAGA+CGG + Chr4:72166372-72166391 None:intergenic 30.0%
GAACATATGAGAAACATGTA+GGG - Chr4:72166490-72166509 MsG0480022407.01.T01:intron 30.0%
GAACATCATAAAACTACACA+AGG + Chr4:72166761-72166780 None:intergenic 30.0%
TCTCAACCATCAAAAAAACT+TGG + Chr4:72166138-72166157 None:intergenic 30.0%
TGAACATATGAGAAACATGT+AGG - Chr4:72166489-72166508 MsG0480022407.01.T01:intron 30.0%
TGACTATTTACAATCTGACA+CGG + Chr4:72167281-72167300 None:intergenic 30.0%
TTAGAGATGAACTATGTTTG+TGG - Chr4:72168016-72168035 MsG0480022407.01.T01:intron 30.0%
TTGTCTATGTTGGAAAAACT+TGG - Chr4:72166659-72166678 MsG0480022407.01.T01:intron 30.0%
! AAAATTGTTTTCTCACTACG+TGG + Chr4:72167602-72167621 None:intergenic 30.0%
! AAATTGTTTTCTCACTACGT+GGG + Chr4:72167601-72167620 None:intergenic 30.0%
! GTAGTGAGAAAACAATTTTC+TGG - Chr4:72167603-72167622 MsG0480022407.01.T01:intron 30.0%
! TCAAGAGAATTTTCACAAAG+AGG - Chr4:72168058-72168077 MsG0480022407.01.T01:intron 30.0%
!! ATAATGGATGAATATGTCTG+TGG - Chr4:72166308-72166327 MsG0480022407.01.T01:CDS 30.0%
!! GATCATGGATTTATTTTCGT+AGG - Chr4:72166866-72166885 MsG0480022407.01.T01:intron 30.0%
!! TTTCTTTATGTGATGGATCA+TGG - Chr4:72166851-72166870 MsG0480022407.01.T01:intron 30.0%
!!! TTGCATGCTTTTAGTTTGTA+TGG - Chr4:72168557-72168576 MsG0480022407.01.T01:CDS 30.0%
AAAACAGATCCTACGAACTA+TGG + Chr4:72166264-72166283 None:intergenic 35.0%
AAAGATTATTGAGGAAGCTG+AGG - Chr4:72167849-72167868 MsG0480022407.01.T01:intron 35.0%
AAAGTTGTGCTTCAGTTGAA+AGG + Chr4:72167007-72167026 None:intergenic 35.0%
AAGAGGCTGACAAAAATTAC+TGG - Chr4:72168075-72168094 MsG0480022407.01.T01:intron 35.0%
AAGTTGTGCTTCAGTTGAAA+GGG + Chr4:72167006-72167025 None:intergenic 35.0%
AATAACGGTTGAGCAAATGA+AGG - Chr4:72167444-72167463 MsG0480022407.01.T01:intron 35.0%
ACAAGGTTCAAAACAGAGAA+AGG - Chr4:72167920-72167939 MsG0480022407.01.T01:intron 35.0%
AGGATTATGAATCAGGGATT+AGG - Chr4:72166036-72166055 MsG0480022407.01.T01:CDS 35.0%
ATATGTCTGTGGATCAATTG+GGG - Chr4:72166319-72166338 MsG0480022407.01.T01:CDS 35.0%
ATGAACCAGAAGATCAAAGT+AGG - Chr4:72167146-72167165 MsG0480022407.01.T01:intron 35.0%
ATGGAAAATGGAAACGGTTA+TGG - Chr4:72165783-72165802 MsG0480022407.01.T01:CDS 35.0%
CAAGGTTCAAAACAGAGAAA+GGG - Chr4:72167921-72167940 MsG0480022407.01.T01:intron 35.0%
CGTCTGTTAAACTTAGATCT+AGG - Chr4:72166370-72166389 MsG0480022407.01.T01:CDS 35.0%
GAAACAGCAACTAAACCAAA+TGG - Chr4:72168332-72168351 MsG0480022407.01.T01:CDS 35.0%
GAATATGTCTGTGGATCAAT+TGG - Chr4:72166317-72166336 MsG0480022407.01.T01:CDS 35.0%
GAGTTCACTAAATCAGCATA+TGG + Chr4:72166170-72166189 None:intergenic 35.0%
GTAAGGTCAAAGAAAGCAAT+AGG - Chr4:72167490-72167509 MsG0480022407.01.T01:intron 35.0%
GTTTGTGGAACAGTTATACT+TGG - Chr4:72168031-72168050 MsG0480022407.01.T01:intron 35.0%
TCAAAACAGAGAAAGGGAAA+AGG - Chr4:72167927-72167946 MsG0480022407.01.T01:intron 35.0%
TCTATCCTACTTTGATCTTC+TGG + Chr4:72167154-72167173 None:intergenic 35.0%
TGAATCTACAGTACCGTTAT+TGG + Chr4:72168423-72168442 None:intergenic 35.0%
TGAGAAGAAAAGAGGCAAAA+AGG - Chr4:72168139-72168158 MsG0480022407.01.T01:intron 35.0%
TGGTGTATGACAAGAAGTTA+TGG - Chr4:72167551-72167570 MsG0480022407.01.T01:intron 35.0%
! AAGTGGGTTTCTATGAGAAA+AGG - Chr4:72167878-72167897 MsG0480022407.01.T01:intron 35.0%
! CATGTGATGGAAAATGGAAA+CGG - Chr4:72165777-72165796 MsG0480022407.01.T01:CDS 35.0%
! GTTTATGCGGGTTGAAATAA+TGG - Chr4:72166292-72166311 MsG0480022407.01.T01:CDS 35.0%
! GTTTTGCATGCTTGTTTATG+CGG - Chr4:72166279-72166298 MsG0480022407.01.T01:intron 35.0%
! TTTTGCATGCTTGTTTATGC+GGG - Chr4:72166280-72166299 MsG0480022407.01.T01:CDS 35.0%
!! ATGCAGAGCTTATAGTTTTG+TGG + Chr4:72167240-72167259 None:intergenic 35.0%
!!! CCGAAACCAAGTTTTTTTGA+TGG - Chr4:72166129-72166148 MsG0480022407.01.T01:CDS 35.0%
!!! CTACAGCCTTTTTTAATCGA+TGG + Chr4:72166910-72166929 None:intergenic 35.0%
AACTAGACGAGAAGGAGAAA+AGG - Chr4:72167809-72167828 MsG0480022407.01.T01:intron 40.0%
AAGGTAACGTTCTTAGGGAA+TGG - Chr4:72165877-72165896 MsG0480022407.01.T01:CDS 40.0%
ACCATTGTGAGTGCTTCTAA+CGG - Chr4:72166601-72166620 MsG0480022407.01.T01:intron 40.0%
ACGTGTTGCAACAATGAGAT+AGG + Chr4:72166783-72166802 None:intergenic 40.0%
ACTAGACGAGAAGGAGAAAA+GGG - Chr4:72167810-72167829 MsG0480022407.01.T01:intron 40.0%
AGTTTCTACCATGTGATTGG+AGG + Chr4:72166963-72166982 None:intergenic 40.0%
CAAACGAGATCTAGCATAGA+AGG + Chr4:72165924-72165943 None:intergenic 40.0%
GAAAAGAGGCAAAAAGGATC+AGG - Chr4:72168145-72168164 MsG0480022407.01.T01:intron 40.0%
GAAAATGGAAACGGTTATGG+TGG - Chr4:72165786-72165805 MsG0480022407.01.T01:CDS 40.0%
GAAGCTGAGGATTACAAAGT+GGG - Chr4:72167862-72167881 MsG0480022407.01.T01:intron 40.0%
GACGACAACTATTCTGGATA+CGG - Chr4:72165627-72165646 MsG0480022407.01.T01:three_prime_UTR 40.0%
GCCAACATTGAGAAGAAAAG+AGG - Chr4:72168131-72168150 MsG0480022407.01.T01:intron 40.0%
GCTCAATGTTGAGACAAACA+AGG - Chr4:72167903-72167922 MsG0480022407.01.T01:intron 40.0%
GTTCGTATTCCATAGTTCGT+AGG - Chr4:72166252-72166271 MsG0480022407.01.T01:intron 40.0%
TAAACCAAATGGATCAGCAC+CGG - Chr4:72168343-72168362 MsG0480022407.01.T01:CDS 40.0%
TGATTTAGTGAACTCTTGCG+TGG - Chr4:72166175-72166194 MsG0480022407.01.T01:intron 40.0%
TGCTATTCAACTAGACGAGA+AGG - Chr4:72167801-72167820 MsG0480022407.01.T01:intron 40.0%
TGGAATGCAAGTTTAGCAAG+AGG - Chr4:72167060-72167079 MsG0480022407.01.T01:intron 40.0%
TGGAGTTTCTACCATGTGAT+TGG + Chr4:72166966-72166985 None:intergenic 40.0%
! AAGGAATCTGAAACACTCAC+CGG + Chr4:72165905-72165924 None:intergenic 40.0%
! GCCTCTTTTCTTCTCAATGT+TGG + Chr4:72168135-72168154 None:intergenic 40.0%
!!! GGGTTAGTTTTATGCTCTGT+TGG - Chr4:72166212-72166231 MsG0480022407.01.T01:intron 40.0%
AAAGACTCCAAAGGGAAGGA+CGG - Chr4:72168305-72168324 MsG0480022407.01.T01:intron 45.0%
ACTACGTGGGATTGACTACA+AGG + Chr4:72167588-72167607 None:intergenic 45.0%
ACTCTTGACCTCCAATCACA+TGG - Chr4:72166952-72166971 MsG0480022407.01.T01:intron 45.0%
ATATCTGCCGCATCCTAGAA+AGG + Chr4:72168226-72168245 None:intergenic 45.0%
CACCTGCTAAAGACTCCAAA+GGG - Chr4:72168297-72168316 MsG0480022407.01.T01:intron 45.0%
CCAAAGGATGCTGCCAATAA+CGG - Chr4:72168407-72168426 MsG0480022407.01.T01:CDS 45.0%
CTAAGAACGTTACCTTCCTC+CGG + Chr4:72165873-72165892 None:intergenic 45.0%
CTGCTGTTGTGTGATCTACA+GGG + Chr4:72165683-72165702 None:intergenic 45.0%
CTTCAGTTGAAAGGGAGATG+TGG + Chr4:72166998-72167017 None:intergenic 45.0%
GACAACTATTCTGGATACGG+CGG - Chr4:72165630-72165649 MsG0480022407.01.T01:three_prime_UTR 45.0%
GCATCCTTTGGTTGTGATTC+AGG + Chr4:72168398-72168417 None:intergenic 45.0%
GGAAGCTGAGGATTACAAAG+TGG - Chr4:72167861-72167880 MsG0480022407.01.T01:intron 45.0%
GGAGGAAGGTAACGTTCTTA+GGG - Chr4:72165872-72165891 MsG0480022407.01.T01:CDS 45.0%
GGATCAGCACCTAAACCAAA+TGG - Chr4:72168353-72168372 MsG0480022407.01.T01:CDS 45.0%
GTGATCTACAGGGACATCTT+CGG + Chr4:72165673-72165692 None:intergenic 45.0%
GTTTGACAACAATGCAGCGT+AGG - Chr4:72166516-72166535 MsG0480022407.01.T01:intron 45.0%
TCGCTGAATCCGAATACATC+CGG + Chr4:72165717-72165736 None:intergenic 45.0%
TCTCAATGTTGGCAACCTCA+CGG + Chr4:72168124-72168143 None:intergenic 45.0%
TGCACCTGAATCACAACCAA+AGG - Chr4:72168391-72168410 MsG0480022407.01.T01:CDS 45.0%
TGCTAAAGACTCCAAAGGGA+AGG - Chr4:72168301-72168320 MsG0480022407.01.T01:intron 45.0%
TGCTCTGTTGGATTGCAGTT+TGG - Chr4:72166224-72166243 MsG0480022407.01.T01:intron 45.0%
TGTATTCGGATTCAGCGATC+AGG - Chr4:72165719-72165738 MsG0480022407.01.T01:three_prime_UTR 45.0%
TGTGATTCAGGTGCAACTTC+CGG + Chr4:72168386-72168405 None:intergenic 45.0%
! CCAGTGCATGTGATGGAAAA+TGG - Chr4:72165771-72165790 MsG0480022407.01.T01:CDS 45.0%
! CCATTTTCCATCACATGCAC+TGG + Chr4:72165774-72165793 None:intergenic 45.0%
! GAGGTCAAGAGTGTGTGTAA+TGG + Chr4:72166944-72166963 None:intergenic 45.0%
! TTCTTTTCCGTCCTTCCCTT+TGG + Chr4:72168315-72168334 None:intergenic 45.0%
!! ACCGTTAGAAGCACTCACAA+TGG + Chr4:72166605-72166624 None:intergenic 45.0%
!! ATCACATGCACTGGTTCGAA+GGG + Chr4:72165765-72165784 None:intergenic 45.0%
!! GGTGCTGATCCATTTGGTTT+AGG + Chr4:72168344-72168363 None:intergenic 45.0%
!! GGTTTAGGTGCTGATCCATT+TGG + Chr4:72168350-72168369 None:intergenic 45.0%
!! TTCCCTTTGGAGTCTTTAGC+AGG + Chr4:72168302-72168321 None:intergenic 45.0%
!! AAAAATTAAAACAAAATATA+TGG - Chr4:72167040-72167059 MsG0480022407.01.T01:intron 5.0%
!! ATAATTATTAAGAATTTAAA+TGG - Chr4:72167514-72167533 MsG0480022407.01.T01:intron 5.0%
!! TATATATAGTAAAAAATATA+AGG - Chr4:72166550-72166569 MsG0480022407.01.T01:intron 5.0%
AAGGGAGACTGAGCGTAACT+CGG + Chr4:72165747-72165766 None:intergenic 50.0%
AGAATAGTTGTCGTCGTCGC+CGG + Chr4:72165622-72165641 None:intergenic 50.0%
ATCTACAGGGACATCTTCGG+CGG + Chr4:72165670-72165689 None:intergenic 50.0%
CCATCAGTTACTGTCCTCCA+AGG - Chr4:72168176-72168195 MsG0480022407.01.T01:intron 50.0%
CCGAATACATCCGGAGATTC+CGG + Chr4:72165708-72165727 None:intergenic 50.0%
CCGGAATCTCCGGATGTATT+CGG - Chr4:72165705-72165724 MsG0480022407.01.T01:three_prime_UTR 50.0%
CCGTTATTGGCAGCATCCTT+TGG + Chr4:72168410-72168429 None:intergenic 50.0%
CCTTGGAGGACAGTAACTGA+TGG + Chr4:72168179-72168198 None:intergenic 50.0%
CGGAGGAAGGTAACGTTCTT+AGG - Chr4:72165871-72165890 MsG0480022407.01.T01:CDS 50.0%
CTCAATGTTGGCAACCTCAC+GGG + Chr4:72168123-72168142 None:intergenic 50.0%
CTTCGAACCAGTGCATGTGA+TGG - Chr4:72165764-72165783 MsG0480022407.01.T01:CDS 50.0%
GAGGTAGATCACAAGTCTCG+TGG - Chr4:72167079-72167098 MsG0480022407.01.T01:intron 50.0%
GCACCTGCTAAAGACTCCAA+AGG - Chr4:72168296-72168315 MsG0480022407.01.T01:intron 50.0%
GCTGCTGTTGTGTGATCTAC+AGG + Chr4:72165684-72165703 None:intergenic 50.0%
GGAGGAATCATGTGAAGTGG+TGG + Chr4:72168269-72168288 None:intergenic 50.0%
GGAGGAGGAATCATGTGAAG+TGG + Chr4:72168272-72168291 None:intergenic 50.0%
GGGAGATGTGGTGCAATTGT+TGG + Chr4:72166986-72167005 None:intergenic 50.0%
GGTGAAGTGGTCTTCGTTGA+CGG + Chr4:72165598-72165617 None:intergenic 50.0%
TCAATGTTGGCAACCTCACG+GGG + Chr4:72168122-72168141 None:intergenic 50.0%
TTCGTTGACGGTGAAGGACA+TGG + Chr4:72165586-72165605 None:intergenic 50.0%
TTGGAGTCTTTAGCAGGTGC+AGG + Chr4:72168296-72168315 None:intergenic 50.0%
! CGTTTTTGTCTCCGATGGTC+CGG - Chr4:72165821-72165840 MsG0480022407.01.T01:CDS 50.0%
! GACGACGTTTTTGTCTCCGA+TGG - Chr4:72165816-72165835 MsG0480022407.01.T01:CDS 50.0%
!! ACTTCCGGTGCTGATCCATT+TGG + Chr4:72168371-72168390 None:intergenic 50.0%
!! CATCACATGCACTGGTTCGA+AGG + Chr4:72165766-72165785 None:intergenic 50.0%
AACGAAGACCACTTCACCGC+CGG - Chr4:72165600-72165619 MsG0480022407.01.T01:three_prime_UTR 55.0%
ACGTTCTTAGGGAATGGCGC+CGG - Chr4:72165883-72165902 MsG0480022407.01.T01:CDS 55.0%
AGATCACACAACAGCAGCGC+CGG - Chr4:72165686-72165705 MsG0480022407.01.T01:three_prime_UTR 55.0%
CGCATCCTAGAAAGGTCGGT+GGG + Chr4:72168218-72168237 None:intergenic 55.0%
CTGCCGCATCCTAGAAAGGT+CGG + Chr4:72168222-72168241 None:intergenic 55.0%
CTTCCTCCTCCTGCTGAGAT+GGG - Chr4:72165846-72165865 MsG0480022407.01.T01:CDS 55.0%
GAAAGAAGCTCCTGCCACTG+AGG - Chr4:72168448-72168467 MsG0480022407.01.T01:CDS 55.0%
GAGTCTTTAGCAGGTGCAGG+TGG + Chr4:72168293-72168312 None:intergenic 55.0%
GCAAACCCACCGACCTTTCT+AGG - Chr4:72168210-72168229 MsG0480022407.01.T01:intron 55.0%
GGCGACGACGACAACTATTC+TGG - Chr4:72165621-72165640 MsG0480022407.01.T01:three_prime_UTR 55.0%
GTGGCAGGAGCTTCTTTCTG+AGG + Chr4:72168446-72168465 None:intergenic 55.0%
GTGGTCTTCGTTGACGGTGA+AGG + Chr4:72165592-72165611 None:intergenic 55.0%
TCTTTAGCAGGTGCAGGTGG+AGG + Chr4:72168290-72168309 None:intergenic 55.0%
TGGGGAGATAATTCCCCGTG+AGG - Chr4:72168106-72168125 MsG0480022407.01.T01:intron 55.0%
TTCCTCCTCCTGCTGAGATG+GGG - Chr4:72165847-72165866 MsG0480022407.01.T01:CDS 55.0%
!! CCACCGACCTTTCTAGGATG+CGG - Chr4:72168216-72168235 MsG0480022407.01.T01:intron 55.0%
!! GCGTGGTGACGAGCTTTTGA+GGG - Chr4:72166192-72166211 MsG0480022407.01.T01:intron 55.0%
!! TGCGTGGTGACGAGCTTTTG+AGG - Chr4:72166191-72166210 MsG0480022407.01.T01:intron 55.0%
ATAGTTGTCGTCGTCGCCGG+CGG + Chr4:72165619-72165638 None:intergenic 60.0%
CCGCATCCTAGAAAGGTCGG+TGG + Chr4:72168219-72168238 None:intergenic 60.0%
GCTTCCTCCTCCTGCTGAGA+TGG - Chr4:72165845-72165864 MsG0480022407.01.T01:CDS 60.0%
TGCAGCAGACTGCTCCTCAG+TGG + Chr4:72168465-72168484 None:intergenic 60.0%
TTAGCAGGTGCAGGTGGAGG+AGG + Chr4:72168287-72168306 None:intergenic 60.0%
! AACAGCAGCGCCGGAATCTC+CGG - Chr4:72165695-72165714 MsG0480022407.01.T01:three_prime_UTR 60.0%
! CGGTGGGTTTGCCTGGCTTT+GGG + Chr4:72168202-72168221 None:intergenic 60.0%
! GAAAGGTCGGTGGGTTTGCC+TGG + Chr4:72168209-72168228 None:intergenic 60.0%
! TCAGCAGGAGGAGGAAGCAC+CGG + Chr4:72165843-72165862 None:intergenic 60.0%
! TCGGTGGGTTTGCCTGGCTT+TGG + Chr4:72168203-72168222 None:intergenic 60.0%
!! AGGAGGAAGCACCGGACCAT+CGG + Chr4:72165835-72165854 None:intergenic 60.0%
!! GTTTGCCTGGCTTTGGGCCT+TGG + Chr4:72168196-72168215 None:intergenic 60.0%
GCAGACTGCTCCTCAGTGGC+AGG + Chr4:72168461-72168480 None:intergenic 65.0%
GTCCTCCAAGGCCCAAAGCC+AGG - Chr4:72168188-72168207 MsG0480022407.01.T01:intron 65.0%
TCCTCCTGCTGAGATGGGGC+CGG - Chr4:72165851-72165870 MsG0480022407.01.T01:CDS 65.0%
!! TGCCTGGCTTTGGGCCTTGG+AGG + Chr4:72168193-72168212 None:intergenic 65.0%
GCTGAGATGGGGCCGGAGGA+AGG - Chr4:72165858-72165877 MsG0480022407.01.T01:CDS 70.0%
GGCCCCATCTCAGCAGGAGG+AGG + Chr4:72165852-72165871 None:intergenic 70.0%
TCCGGCCCCATCTCAGCAGG+AGG + Chr4:72165855-72165874 None:intergenic 70.0%
TCCTCCGGCCCCATCTCAGC+AGG + Chr4:72165858-72165877 None:intergenic 70.0%
TCCTGCTGAGATGGGGCCGG+AGG - Chr4:72165854-72165873 MsG0480022407.01.T01:CDS 70.0%
CGTCGTCGCCGGCGGTGAAG+TGG + Chr4:72165611-72165630 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 72165509 72168719 72165509 ID=MsG0480022407.01;Name=MsG0480022407.01
Chr4 mRNA 72165509 72168719 72165509 ID=MsG0480022407.01.T01;Parent=MsG0480022407.01;Name=MsG0480022407.01.T01;_AED=0.31;_eAED=0.31;_QI=76|1|1|1|1|1|3|232|305
Chr4 exon 72165509 72166184 72165509 ID=MsG0480022407.01.T01:exon:31016;Parent=MsG0480022407.01.T01
Chr4 exon 72166280 72166433 72166280 ID=MsG0480022407.01.T01:exon:31015;Parent=MsG0480022407.01.T01
Chr4 exon 72168324 72168719 72168324 ID=MsG0480022407.01.T01:exon:31014;Parent=MsG0480022407.01.T01
Chr4 five_prime_UTR 72168644 72168719 72168644 ID=MsG0480022407.01.T01:five_prime_utr;Parent=MsG0480022407.01.T01
Chr4 CDS 72168324 72168643 72168324 ID=MsG0480022407.01.T01:cds;Parent=MsG0480022407.01.T01
Chr4 CDS 72166280 72166433 72166280 ID=MsG0480022407.01.T01:cds;Parent=MsG0480022407.01.T01
Chr4 CDS 72165741 72166184 72165741 ID=MsG0480022407.01.T01:cds;Parent=MsG0480022407.01.T01
Chr4 three_prime_UTR 72165509 72165740 72165509 ID=MsG0480022407.01.T01:three_prime_utr;Parent=MsG0480022407.01.T01
Gene Sequence

>MsG0480022407.01.T01

ATGTCCTTCACCGTCAACGAAGACCACTTCACCGCCGGCGACGACGACAACTATTCTGGATACGGCGGATTCTCAAGCTACTCCGCCGAAGATGTCCCTGTAGATCACACAACAGCAGCGCCGGAATCTCCGGATGTATTCGGATTCAGCGATCAGGATCCGAGTTACGCTCAGTCTCCCTTCGAACCAGTGCATGTGATGGAAAATGGAAACGGTTATGGTGGTCACGATGACGACGTTTTTGTCTCCGATGGTCCGGTGCTTCCTCCTCCTGCTGAGATGGGGCCGGAGGAAGGTAACGTTCTTAGGGAATGGCGCCGTCAAAATGCTATTCAACTAGACGAGAAGGAGAAAAGGGAAAAAGAAATGAGATTAAAGATTATTGAGGAAGCTGAGGATTACAAAGTGGGTTTCTATGAGAAAAGGAAGCTCAATGTTGAGACAAACAAGGTTCAAAACAGAGAAAGGGAAAAGTTATACTTGGCCAATCAAGAGAATTTTCACAAAGAGGCTGACAAAAATTACTGGAAAGCAATTGGGGAGATAATTCCCCGTGAGGTTGCCAACATTGAGAAGAAAAGAGGCAAAAAGGATCAGGATAAGAATCCATCAGTTACTGTCCTCCAAGGCCCAAAGCCAGGCAAACCCACCGACCTTTCTAGGATGCGGCAGATATTGTTAAAGCTGAAACATACGCCACCACTTCACATGATTCCTCCTCCACCTGCACCTGCTAAAGACTCCAAAGGGAAGGACGGAAAAGAAACAGCAACTAAACCAAATGGATCAGCACCTAAACCAAATGGATCAGCACCGGAAGTTGCACCTGAATCACAACCAAAGGATGCTGCCAATAACGGTACTGTAGATTCACCTCAGAAAGAAGCTCCTGCCACTGAGGAGCAGTCTGCTGCATAA
Protein sequence

>MsG0480022407.01.T01

MSFTVNEDHFTAGDDDNYSGYGGFSSYSAEDVPVDHTTAAPESPDVFGFSDQDPSYAQSPFEPVHVMENGNGYGGHDDDVFVSDGPVLPPPAEMGPEEGNVLREWRRQNAIQLDEKEKREKEMRLKIIEEAEDYKVGFYEKRKLNVETNKVQNREREKLYLANQENFHKEADKNYWKAIGEIIPREVANIEKKRGKKDQDKNPSVTVLQGPKPGKPTDLSRMRQILLKLKHTPPLHMIPPPPAPAKDSKGKDGKETATKPNGSAPKPNGSAPEVAPESQPKDAANNGTVDSPQKEAPATEEQSAA*