Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002226.01.T01 | YP_009572825.1 | 96.774 | 155 | 5 | 0 | 1 | 155 | 1 | 155 | 4.70E-104 | 304 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002226.01.T01 | Q9B1A1 | 94.194 | 155 | 9 | 0 | 1 | 155 | 1 | 155 | 4.27E-105 | 300 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002226.01.T01 | A0A0K0LTL2 | 96.774 | 155 | 5 | 0 | 1 | 155 | 1 | 155 | 2.24e-104 | 304 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048698.01 | MsG0180002226.01 | 0.841980 | 3.418817e-58 | 5.109447e-55 |
| MsG0080048699.01 | MsG0180002226.01 | 0.808163 | 3.544239e-50 | 2.012010e-47 |
| MsG0180002226.01 | MsG0180002435.01 | 0.816247 | 6.100943e-52 | 4.300636e-49 |
| MsG0180002226.01 | MsG0280007042.01 | 0.806605 | 7.584242e-50 | 4.134065e-47 |
| MsG0180002226.01 | MsG0280010145.01 | 0.830616 | 2.656851e-55 | 2.813839e-52 |
| MsG0180002226.01 | MsG0380013448.01 | 0.802943 | 4.412623e-49 | 2.189452e-46 |
| MsG0180002226.01 | MsG0380013915.01 | 0.847140 | 1.396316e-59 | 2.458545e-56 |
| MsG0180002226.01 | MsG0380013916.01 | 0.875105 | 4.005187e-68 | 1.847770e-64 |
| MsG0180002226.01 | MsG0380013917.01 | 0.835980 | 1.223017e-56 | 1.520159e-53 |
| MsG0180002226.01 | MsG0380013918.01 | 0.800298 | 1.539221e-48 | 7.141773e-46 |
| MsG0180002226.01 | MsG0380013920.01 | 0.903586 | 2.976721e-79 | 4.434246e-75 |
| MsG0180002226.01 | MsG0380013923.01 | 0.828909 | 6.916994e-55 | 6.969426e-52 |
| MsG0180002226.01 | MsG0380013929.01 | 0.826912 | 2.090197e-54 | 1.987250e-51 |
| MsG0180002226.01 | MsG0380013931.01 | 0.850015 | 2.231350e-60 | 4.310694e-57 |
| MsG0180002226.01 | MsG0380013932.01 | 0.826357 | 2.835695e-54 | 2.653332e-51 |
| MsG0180002226.01 | MsG0380013939.01 | 0.807075 | 6.034618e-50 | 3.329472e-47 |
| MsG0180002226.01 | MsG0380013942.01 | 0.830191 | 3.375194e-55 | 3.530902e-52 |
| MsG0180002226.01 | MsG0380013944.01 | 0.837771 | 4.268684e-57 | 5.602905e-54 |
| MsG0180002226.01 | MsG0380013947.01 | 0.867617 | 1.199676e-65 | 4.215130e-62 |
| MsG0180002226.01 | MsG0380013948.01 | 0.874258 | 7.770243e-68 | 3.474502e-64 |
| MsG0180002226.01 | MsG0380013949.01 | 0.877701 | 5.089575e-69 | 2.584448e-65 |
| MsG0180002226.01 | MsG0380013951.01 | 0.809150 | 2.181376e-50 | 1.270970e-47 |
| MsG0180002226.01 | MsG0380013952.01 | 0.842231 | 2.933843e-58 | 4.419246e-55 |
| MsG0180002226.01 | MsG0380013964.01 | 0.822840 | 1.910275e-53 | 1.617303e-50 |
| MsG0180002226.01 | MsG0380013968.01 | 0.807046 | 6.119431e-50 | 3.373821e-47 |
| MsG0180002226.01 | MsG0380013969.01 | 0.831785 | 1.370938e-55 | 1.503341e-52 |
| MsG0180002226.01 | MsG0380013972.01 | 0.852427 | 4.649003e-61 | 9.710932e-58 |
| MsG0180002226.01 | MsG0380013980.01 | 0.846461 | 2.141166e-59 | 3.688522e-56 |
| MsG0180002226.01 | MsG0380013981.01 | 0.840257 | 9.696360e-58 | 1.373457e-54 |
| MsG0180002226.01 | MsG0380013983.01 | 0.807536 | 4.819085e-50 | 2.690951e-47 |
| MsG0180002226.01 | MsG0380013995.01 | 0.839971 | 1.151502e-57 | 1.616761e-54 |
| MsG0180002226.01 | MsG0380014008.01 | 0.842780 | 2.097415e-58 | 3.214107e-55 |
| MsG0180002226.01 | MsG0380014009.01 | 0.804190 | 2.432939e-49 | 1.246431e-46 |
| MsG0180002226.01 | MsG0380014011.01 | 0.837542 | 4.888186e-57 | 6.370005e-54 |
| MsG0180002226.01 | MsG0380014018.01 | 0.819832 | 9.443215e-53 | 7.347146e-50 |
| MsG0180002226.01 | MsG0380014030.01 | 0.827308 | 1.680508e-54 | 1.616341e-51 |
| MsG0180002226.01 | MsG0380014036.01 | 0.827603 | 1.428493e-54 | 1.385546e-51 |
| MsG0180002226.01 | MsG0380014039.01 | 0.808555 | 2.923412e-50 | 1.676665e-47 |
| MsG0180002226.01 | MsG0380014040.01 | 0.832314 | 1.014319e-55 | 1.129425e-52 |
| MsG0180002226.01 | MsG0380014041.01 | 0.811151 | 8.079431e-51 | 4.963528e-48 |
| MsG0180002226.01 | MsG0380014044.01 | 0.819870 | 9.255448e-53 | 7.208685e-50 |
| MsG0180002226.01 | MsG0380014048.01 | 0.861275 | 1.150322e-63 | 3.237201e-60 |
| MsG0180002226.01 | MsG0380014051.01 | 0.821710 | 3.493940e-53 | 2.864381e-50 |
| MsG0180002226.01 | MsG0380014055.01 | 0.801242 | 9.874235e-49 | 4.692497e-46 |
| MsG0180002226.01 | MsG0380014057.01 | 0.824114 | 9.617659e-54 | 8.440391e-51 |
| MsG0180002226.01 | MsG0380017007.01 | 0.830647 | 2.609919e-55 | 2.766746e-52 |
| MsG0180002226.01 | MsG0580028230.01 | 0.816619 | 5.036378e-52 | 3.586455e-49 |
| MsG0180002226.01 | MsG0680032339.01 | 0.863857 | 1.845575e-64 | 5.677251e-61 |
| MsG0180002226.01 | MsG0780038140.01 | 0.812548 | 4.010912e-51 | 2.557729e-48 |
| MsG0180002226.01 | MsG0780040316.01 | 0.801736 | 7.821866e-49 | 3.763529e-46 |
| MsG0180002226.01 | MsG0780040317.01 | 0.805316 | 1.415853e-49 | 7.467161e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002226.01.T01 | MTR_3g035660 | 89.404 | 151 | 14 | 1 | 3 | 151 | 160 | 310 | 3.33e-87 | 272 |
| MsG0180002226.01.T01 | MTR_0002s0300 | 79.545 | 132 | 26 | 1 | 25 | 155 | 1 | 132 | 8.25e-70 | 207 |
| MsG0180002226.01.T01 | MTR_4g103280 | 87.356 | 87 | 11 | 0 | 66 | 152 | 22 | 108 | 9.31e-48 | 152 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002226.01.T01 | ATCG01240 | 92.903 | 155 | 11 | 0 | 1 | 155 | 1 | 155 | 5.03e-105 | 297 |
| MsG0180002226.01.T01 | ATCG00900 | 92.903 | 155 | 11 | 0 | 1 | 155 | 1 | 155 | 5.03e-105 | 297 |
| MsG0180002226.01.T01 | ATMG01270 | 31.429 | 140 | 86 | 3 | 22 | 155 | 11 | 146 | 3.20e-15 | 68.9 |
| MsG0180002226.01.T01 | AT2G07696 | 31.429 | 140 | 86 | 3 | 22 | 155 | 11 | 146 | 3.20e-15 | 68.9 |
Find 25 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTAATCGATTACGATAAAT+TGG | 0.238141 | 1:+35094514 | None:intergenic |
| CTTGCCATTCGTTGGTTATT+AGG | 0.268444 | 1:-35094265 | MsG0180002226.01.T01:CDS |
| CATCAAGTTCACGTTGAAAT+AGG | 0.304216 | 1:-35094307 | MsG0180002226.01.T01:CDS |
| AAAACGTCCGGGTCGAAATA+TGG | 0.311130 | 1:-35094233 | MsG0180002226.01.T01:CDS |
| CAAATTAAGTTCCGAATTAG+TGG | 0.342047 | 1:-35094206 | MsG0180002226.01.T01:CDS |
| TGCCGATGCCATACGCAAAA+AGG | 0.355357 | 1:-35094167 | MsG0180002226.01.T01:CDS |
| TTTGGCAACATCCACTAATT+CGG | 0.408012 | 1:+35094195 | None:intergenic |
| TATGGCATCGGCACTCCCTT+TGG | 0.413652 | 1:+35094177 | None:intergenic |
| GCTTGACGTAAAACAGATAG+TGG | 0.426676 | 1:+35094388 | None:intergenic |
| AGGCGCATCCCGAAAACGTC+CGG | 0.430208 | 1:-35094245 | MsG0180002226.01.T01:CDS |
| TTATCTATCGAGCTATGAAA+AGG | 0.512086 | 1:-35094435 | MsG0180002226.01.T01:CDS |
| GAAAAGCACTTGCCATTCGT+TGG | 0.526859 | 1:-35094273 | MsG0180002226.01.T01:CDS |
| TAATCGATTAGTTAACATGT+TGG | 0.538486 | 1:-35094503 | MsG0180002226.01.T01:CDS |
| AAAGGAAGAGACTCATAGAA+TGG | 0.548096 | 1:-35094149 | MsG0180002226.01.T01:CDS |
| GGCGCATCCCGAAAACGTCC+GGG | 0.548729 | 1:-35094244 | MsG0180002226.01.T01:CDS |
| GTAAAAGCAAGACGCGTAAG+CGG | 0.560461 | 1:-35094337 | MsG0180002226.01.T01:CDS |
| GAATTAGTGGATGTTGCCAA+AGG | 0.563584 | 1:-35094193 | MsG0180002226.01.T01:CDS |
| AATTAGTGGATGTTGCCAAA+GGG | 0.582053 | 1:-35094192 | MsG0180002226.01.T01:CDS |
| AGAGACTCATAGAATGGCAG+AGG | 0.593635 | 1:-35094143 | MsG0180002226.01.T01:CDS |
| TGCGCCTAATAACCAACGAA+TGG | 0.620213 | 1:+35094261 | None:intergenic |
| TTGAAAGCCATATTTCGACC+CGG | 0.633461 | 1:+35094226 | None:intergenic |
| GTTAACCGTATTATGAAACA+CGG | 0.650772 | 1:-35094481 | MsG0180002226.01.T01:CDS |
| TTTACGCTCATGTCACGGCG+AGG | 0.672550 | 1:-35094568 | None:intergenic |
| TTTACGTCAAGCAATACGTG+GGG | 0.729232 | 1:-35094377 | MsG0180002226.01.T01:CDS |
| GTTGAAATAGGATCCACACA+AGG | 0.756933 | 1:-35094295 | MsG0180002226.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACTAATCGATTACGATAAAT+TGG | + | Chr1:35094162-35094181 | None:intergenic | 25.0% |
| !! | TAATCGATTAGTTAACATGT+TGG | - | Chr1:35094170-35094189 | MsG0180002226.01.T01:CDS | 25.0% |
| !! | TTTTTCCGTGTTTCATAATA+CGG | + | Chr1:35094200-35094219 | None:intergenic | 25.0% |
| CAAATTAAGTTCCGAATTAG+TGG | - | Chr1:35094467-35094486 | MsG0180002226.01.T01:CDS | 30.0% | |
| GAAACACGGAAAAAAATCAT+TGG | - | Chr1:35094206-35094225 | MsG0180002226.01.T01:CDS | 30.0% | |
| GTTAACCGTATTATGAAACA+CGG | - | Chr1:35094192-35094211 | MsG0180002226.01.T01:CDS | 30.0% | |
| TTATCTATCGAGCTATGAAA+AGG | - | Chr1:35094238-35094257 | MsG0180002226.01.T01:CDS | 30.0% | |
| ! | AGGTACTGCAGAAAAAAAAA+CGG | - | Chr1:35094125-35094144 | MsG0180002226.01.T01:CDS | 30.0% |
| !! | TCTTGCTTTTACTGGTATAT+CGG | + | Chr1:35094328-35094347 | None:intergenic | 30.0% |
| AAAGGAAGAGACTCATAGAA+TGG | - | Chr1:35094524-35094543 | MsG0180002226.01.T01:CDS | 35.0% | |
| CATCAAGTTCACGTTGAAAT+AGG | - | Chr1:35094366-35094385 | MsG0180002226.01.T01:CDS | 35.0% | |
| TTTGGCAACATCCACTAATT+CGG | + | Chr1:35094481-35094500 | None:intergenic | 35.0% | |
| ! | AATTAGTGGATGTTGCCAAA+GGG | - | Chr1:35094481-35094500 | MsG0180002226.01.T01:CDS | 35.0% |
| ! | TTTTACGTCAAGCAATACGT+GGG | - | Chr1:35094295-35094314 | MsG0180002226.01.T01:CDS | 35.0% |
| !! | CTTGCTTTTACTGGTATATC+GGG | + | Chr1:35094327-35094346 | None:intergenic | 35.0% |
| GCTTGACGTAAAACAGATAG+TGG | + | Chr1:35094288-35094307 | None:intergenic | 40.0% | |
| GTTGAAATAGGATCCACACA+AGG | - | Chr1:35094378-35094397 | MsG0180002226.01.T01:CDS | 40.0% | |
| TTGAAAGCCATATTTCGACC+CGG | + | Chr1:35094450-35094469 | None:intergenic | 40.0% | |
| TTTACGTCAAGCAATACGTG+GGG | - | Chr1:35094296-35094315 | MsG0180002226.01.T01:CDS | 40.0% | |
| ! | AGTCTCTTCCTTTTTGCGTA+TGG | + | Chr1:35094517-35094536 | None:intergenic | 40.0% |
| ! | CTTGCCATTCGTTGGTTATT+AGG | - | Chr1:35094408-35094427 | MsG0180002226.01.T01:CDS | 40.0% |
| ! | GAATTAGTGGATGTTGCCAA+AGG | - | Chr1:35094480-35094499 | MsG0180002226.01.T01:CDS | 40.0% |
| ! | GTTTTACGTCAAGCAATACG+TGG | - | Chr1:35094294-35094313 | MsG0180002226.01.T01:CDS | 40.0% |
| ! | TTCCTTTTTGCGTATGGCAT+CGG | + | Chr1:35094511-35094530 | None:intergenic | 40.0% |
| AAAACGTCCGGGTCGAAATA+TGG | - | Chr1:35094440-35094459 | MsG0180002226.01.T01:CDS | 45.0% | |
| AGAGACTCATAGAATGGCAG+AGG | - | Chr1:35094530-35094549 | MsG0180002226.01.T01:CDS | 45.0% | |
| GTAAAAGCAAGACGCGTAAG+CGG | - | Chr1:35094336-35094355 | MsG0180002226.01.T01:CDS | 45.0% | |
| TGCGCCTAATAACCAACGAA+TGG | + | Chr1:35094415-35094434 | None:intergenic | 45.0% | |
| ! | TATTTCGACCCGGACGTTTT+CGG | + | Chr1:35094440-35094459 | None:intergenic | 45.0% |
| !! | CTTACGCGTCTTGCTTTTAC+TGG | + | Chr1:35094336-35094355 | None:intergenic | 45.0% |
| !! | GAAAAGCACTTGCCATTCGT+TGG | - | Chr1:35094400-35094419 | MsG0180002226.01.T01:CDS | 45.0% |
| TGCCGATGCCATACGCAAAA+AGG | - | Chr1:35094506-35094525 | MsG0180002226.01.T01:CDS | 50.0% | |
| ! | ATTTCGACCCGGACGTTTTC+GGG | + | Chr1:35094439-35094458 | None:intergenic | 50.0% |
| ! | GGCAAGTGCTTTTCCTTGTG+TGG | + | Chr1:35094394-35094413 | None:intergenic | 50.0% |
| !! | TATGGCATCGGCACTCCCTT+TGG | + | Chr1:35094499-35094518 | None:intergenic | 55.0% |
| AGGCGCATCCCGAAAACGTC+CGG | - | Chr1:35094428-35094447 | MsG0180002226.01.T01:CDS | 60.0% | |
| GGCGCATCCCGAAAACGTCC+GGG | - | Chr1:35094429-35094448 | MsG0180002226.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 35094114 | 35094581 | 35094114 | ID=MsG0180002226.01;Name=MsG0180002226.01 |
| Chr1 | mRNA | 35094114 | 35094581 | 35094114 | ID=MsG0180002226.01.T01;Parent=MsG0180002226.01;Name=MsG0180002226.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|155 |
| Chr1 | exon | 35094114 | 35094581 | 35094114 | ID=MsG0180002226.01.T01:exon:46119;Parent=MsG0180002226.01.T01 |
| Chr1 | CDS | 35094114 | 35094581 | 35094114 | ID=MsG0180002226.01.T01:cds;Parent=MsG0180002226.01.T01 |
| Gene Sequence |
| Protein sequence |
