AlfalfaGEDB | MsG0380014041.01

M. sativa cultivar ZhongmuNo.1 / MsG0380014041.01

KEGG: K02982
GO: GO:0003723 GO:0003735 GO:0005840 GO:0006412 GO:0015935
PFAM: PF00189

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014041.01.T01	YP_009141647.1	100	141	0	0	1	141	74	214	8.82E-96	285

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014041.01.T01	Q9BBP8	87.943	141	17	0	1	141	78	218	1.09E-86	254

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014041.01.T01	A0A4P8F265	100.000	141	0	0	1	141	74	214	4.21e-96	285

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048689.01	MsG0380014041.01	0.836284	1.024102e-56	1.284817e-53
MsG0080049079.01	MsG0380014041.01	0.822372	2.454026e-53	2.050324e-50
MsG0080049080.01	MsG0380014041.01	0.812416	4.286431e-51	2.723941e-48
MsG0180002226.01	MsG0380014041.01	0.811151	8.079431e-51	4.963528e-48
MsG0180002435.01	MsG0380014041.01	0.843581	1.282808e-58	2.016305e-55
MsG0380013889.01	MsG0380014041.01	0.803211	3.884898e-49	1.940873e-46
MsG0380013916.01	MsG0380014041.01	0.814497	1.495346e-51	1.005001e-48
MsG0380013917.01	MsG0380014041.01	0.841335	5.057710e-58	7.407136e-55
MsG0380013918.01	MsG0380014041.01	0.844454	7.483948e-59	1.208775e-55
MsG0380013920.01	MsG0380014041.01	0.812012	5.251740e-51	3.300843e-48
MsG0380013930.01	MsG0380014041.01	0.815548	8.738404e-52	6.042883e-49
MsG0380013931.01	MsG0380014041.01	0.808164	3.543396e-50	2.011566e-47
MsG0380013932.01	MsG0380014041.01	0.869142	3.865149e-66	1.434988e-62
MsG0380013939.01	MsG0380014041.01	0.806610	7.567207e-50	4.125229e-47
MsG0380013944.01	MsG0380014041.01	0.817385	3.389063e-52	2.464641e-49
MsG0380013948.01	MsG0380014041.01	0.811433	7.017855e-51	4.343654e-48
MsG0380013949.01	MsG0380014041.01	0.835852	1.318113e-56	1.631947e-53
MsG0380013950.01	MsG0380014041.01	0.830761	2.448063e-55	2.603861e-52
MsG0380013952.01	MsG0380014041.01	0.824532	7.670099e-54	6.812538e-51
MsG0380013958.01	MsG0380014041.01	0.831391	1.713943e-55	1.857379e-52
MsG0380013959.01	MsG0380014041.01	0.859088	5.266476e-63	1.374140e-59
MsG0380013964.01	MsG0380014041.01	0.844612	6.788941e-59	1.102011e-55
MsG0380013965.01	MsG0380014041.01	0.805375	1.376208e-49	7.269062e-47
MsG0380013969.01	MsG0380014041.01	0.820115	8.135251e-53	6.380685e-50
MsG0380013972.01	MsG0380014041.01	0.853142	2.905592e-61	6.215235e-58
MsG0380013978.01	MsG0380014041.01	0.804152	2.477316e-49	1.267983e-46
MsG0380013979.01	MsG0380014041.01	0.817705	2.871581e-52	2.106575e-49
MsG0380013980.01	MsG0380014041.01	0.851005	1.175859e-60	2.346664e-57
MsG0380013981.01	MsG0380014041.01	0.868080	8.518159e-66	3.041987e-62
MsG0380013983.01	MsG0380014041.01	0.831425	1.681586e-55	1.824050e-52
MsG0380013988.01	MsG0380014041.01	0.944934	7.812032e-104	1.154529e-98
MsG0380013995.01	MsG0380014041.01	0.865486	5.701639e-65	1.858182e-61
MsG0380013996.01	MsG0380014041.01	0.804862	1.761804e-49	9.184523e-47
MsG0380014008.01	MsG0380014041.01	0.851918	6.489646e-61	1.333611e-57
MsG0380014009.01	MsG0380014041.01	0.995603	6.033759e-218	8.917207e-211
MsG0380014010.01	MsG0380014041.01	0.819206	1.312042e-52	1.003134e-49
MsG0380014011.01	MsG0380014041.01	0.841360	4.981671e-58	7.301989e-55
MsG0380014013.01	MsG0380014041.01	0.812657	3.797499e-51	2.428430e-48
MsG0380014030.01	MsG0380014041.01	0.805628	1.218079e-49	6.475809e-47
MsG0380014034.01	MsG0380014041.01	0.817319	3.507780e-52	2.546141e-49
MsG0380014035.01	MsG0380014041.01	0.841117	5.772549e-58	8.396533e-55
MsG0380014036.01	MsG0380014041.01	0.864861	8.963075e-65	2.855979e-61
MsG0380014037.01	MsG0380014041.01	0.829505	4.958734e-55	5.084869e-52
MsG0380014038.01	MsG0380014041.01	0.800126	1.667740e-48	7.704358e-46
MsG0380014040.01	MsG0380014041.01	0.835596	1.530458e-56	1.880338e-53
MsG0380014041.01	MsG0380014042.01	0.826880	2.127846e-54	2.020976e-51
MsG0380014041.01	MsG0380014044.01	0.861840	7.730689e-64	2.217001e-60
MsG0380014041.01	MsG0380014052.01	0.812661	3.788588e-51	2.423062e-48
MsG0380014041.01	MsG0380014055.01	0.996530	1.010181e-228	1.962140e-221
MsG0380014041.01	MsG0380014056.01	0.858067	1.062648e-62	2.678487e-59
MsG0380014041.01	MsG0380014057.01	0.816136	6.459794e-52	4.539760e-49
MsG0380014041.01	MsG0380017006.01	0.816008	6.899822e-52	4.831990e-49
MsG0380014041.01	MsG0580028230.01	0.805466	1.317139e-49	6.973447e-47
MsG0380014041.01	MsG0780040316.01	0.842754	2.131597e-58	3.263692e-55
MsG0380014041.01	MsG0780040317.01	0.819045	1.427017e-52	1.086298e-49
MsG0380014041.01	MsG0880043541.01	0.817658	2.941433e-52	2.155078e-49
MsG0380014041.01	MsG0880044440.01	0.832670	8.278862e-56	9.317746e-53
MsG0280007041.01	MsG0380014041.01	0.817472	3.240387e-52	2.362154e-49
MsG0280007042.01	MsG0380014041.01	0.868920	4.561592e-66	1.679364e-62

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014041.01.T01	MTR_4g051410	97.163	141	4	0	1	141	74	214	2.19e-95	274
MsG0380014041.01.T01	MTR_8g038470	79.310	116	17	1	13	128	63	171	1.04e-56	176
MsG0380014041.01.T01	MTR_0002s0790	59.574	141	55	1	1	141	101	239	2.10e-56	176

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014041.01.T01	ATCG00800	82.979	141	24	0	1	141	78	218	2.62e-84	246

Find 27 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATACAGTTCGAACTATTTA+TGG	0.120735	3:-48719436	MsG0380014041.01.T01:CDS
ATAAATTCTGCAAGAATATT+AGG	0.153378	3:+48719658	None:intergenic
CGAACTATTTATGGGGTATT+AGG	0.214307	3:-48719427	MsG0380014041.01.T01:CDS
GGGACTTCCAACGTTATTAA+TGG	0.264579	3:-48719794	MsG0380014041.01.T01:CDS
TAAAGAATAGAATTTCGTTT+AGG	0.349086	3:-48719624	MsG0380014041.01.T01:CDS
AGCTATTGAATTAGCTGAAC+AGG	0.363850	3:-48719587	MsG0380014041.01.T01:CDS
ATACAGTTCGAACTATTTAT+GGG	0.369096	3:-48719435	MsG0380014041.01.T01:CDS
GGGTATTAGGAATCAAAGTT+TGG	0.387551	3:-48719414	MsG0380014041.01.T01:CDS
TTAGGGTCGCTATAAGCATT+TGG	0.411303	3:+48719676	None:intergenic
TAAATTCTGCAAGAATATTA+GGG	0.426926	3:+48719659	None:intergenic
ATAAATAGTTCGAACTGTAT+AGG	0.427068	3:+48719438	None:intergenic
GGAGTTCAAGTACAAATTGC+GGG	0.442137	3:-48719553	MsG0380014041.01.T01:CDS
TGTAATTCTTCGATTCTTCG+AGG	0.463402	3:+48719763	None:intergenic
ATTCTTGCAGAATTTATAGC+TGG	0.468432	3:-48719652	MsG0380014041.01.T01:CDS
AAGAAATTGCACGTGTCGAA+TGG	0.483791	3:-48719516	MsG0380014041.01.T01:CDS
GCTGAACAGGCAGGTACAAA+AGG	0.497837	3:-48719574	MsG0380014041.01.T01:CDS
CGAATGGATTAGAGAAGGTA+GGG	0.541012	3:-48719500	MsG0380014041.01.T01:CDS
CAAATTGCGGGACGTATCGA+CGG	0.567491	3:-48719541	MsG0380014041.01.T01:CDS
TTAGCTCGAATGGTTTGTAG+AGG	0.585057	3:+48719475	None:intergenic
ACTTCCAACGTTATTAATGG+AGG	0.585517	3:-48719791	MsG0380014041.01.T01:CDS
TCGAATGGATTAGAGAAGGT+AGG	0.595160	3:-48719501	MsG0380014041.01.T01:CDS
CTTCCAACGTTATTAATGGA+GGG	0.595517	3:-48719790	MsG0380014041.01.T01:CDS
ATTGAATTAGCTGAACAGGC+AGG	0.608696	3:-48719583	MsG0380014041.01.T01:CDS
TTACCCTCCATTAATAACGT+TGG	0.614669	3:+48719787	None:intergenic
CGTGTCGAATGGATTAGAGA+AGG	0.634314	3:-48719505	MsG0380014041.01.T01:CDS
TACAGTTCGAACTATTTATG+GGG	0.637128	3:-48719434	MsG0380014041.01.T01:CDS
AGGAGTTCAAGTACAAATTG+CGG	0.647406	3:-48719554	MsG0380014041.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAATTGTAATGTTAATTTTT+CGG	+	Chr3:48719485-48719504	None:intergenic	10.0%
!!!	AATTGTAATGTTAATTTTTC+GGG	+	Chr3:48719484-48719503	None:intergenic	15.0%
!!	ATAAATTCTGCAAGAATATT+AGG	+	Chr3:48719534-48719553	None:intergenic	20.0%
!!	TAAAGAATAGAATTTCGTTT+AGG	-	Chr3:48719565-48719584	MsG0380014041.01.T01:CDS	20.0%
!!	TAAATTCTGCAAGAATATTA+GGG	+	Chr3:48719533-48719552	None:intergenic	20.0%
!	ATAAATAGTTCGAACTGTAT+AGG	+	Chr3:48719754-48719773	None:intergenic	25.0%
!	ATACAGTTCGAACTATTTAT+GGG	-	Chr3:48719754-48719773	MsG0380014041.01.T01:CDS	25.0%
!	TATACAGTTCGAACTATTTA+TGG	-	Chr3:48719753-48719772	MsG0380014041.01.T01:CDS	25.0%
!!!	ATAATCAATTTTAGCTCGAA+TGG	+	Chr3:48719727-48719746	None:intergenic	25.0%
	ATTCTTGCAGAATTTATAGC+TGG	-	Chr3:48719537-48719556	MsG0380014041.01.T01:CDS	30.0%
	TACAGTTCGAACTATTTATG+GGG	-	Chr3:48719755-48719774	MsG0380014041.01.T01:CDS	30.0%
	ACTTCCAACGTTATTAATGG+AGG	-	Chr3:48719398-48719417	MsG0380014041.01.T01:CDS	35.0%
	AGCTATTGAATTAGCTGAAC+AGG	-	Chr3:48719602-48719621	MsG0380014041.01.T01:CDS	35.0%
	AGGAGTTCAAGTACAAATTG+CGG	-	Chr3:48719635-48719654	MsG0380014041.01.T01:CDS	35.0%
	CTTCCAACGTTATTAATGGA+GGG	-	Chr3:48719399-48719418	MsG0380014041.01.T01:CDS	35.0%
	GGGTATTAGGAATCAAAGTT+TGG	-	Chr3:48719775-48719794	MsG0380014041.01.T01:CDS	35.0%
	TGTAATTCTTCGATTCTTCG+AGG	+	Chr3:48719429-48719448	None:intergenic	35.0%
	TTACCCTCCATTAATAACGT+TGG	+	Chr3:48719405-48719424	None:intergenic	35.0%
!	CGAACTATTTATGGGGTATT+AGG	-	Chr3:48719762-48719781	MsG0380014041.01.T01:CDS	35.0%
	AAGAAATTGCACGTGTCGAA+TGG	-	Chr3:48719673-48719692	MsG0380014041.01.T01:CDS	40.0%
	ATTGAATTAGCTGAACAGGC+AGG	-	Chr3:48719606-48719625	MsG0380014041.01.T01:CDS	40.0%
	GGAGTTCAAGTACAAATTGC+GGG	-	Chr3:48719636-48719655	MsG0380014041.01.T01:CDS	40.0%
	GGGACTTCCAACGTTATTAA+TGG	-	Chr3:48719395-48719414	MsG0380014041.01.T01:CDS	40.0%
	TTAGGGTCGCTATAAGCATT+TGG	+	Chr3:48719516-48719535	None:intergenic	40.0%
!	TTAGCTCGAATGGTTTGTAG+AGG	+	Chr3:48719717-48719736	None:intergenic	40.0%
!!	CGAATGGATTAGAGAAGGTA+GGG	-	Chr3:48719689-48719708	MsG0380014041.01.T01:CDS	40.0%
!!	TCGAATGGATTAGAGAAGGT+AGG	-	Chr3:48719688-48719707	MsG0380014041.01.T01:CDS	40.0%
!!	CGTGTCGAATGGATTAGAGA+AGG	-	Chr3:48719684-48719703	MsG0380014041.01.T01:CDS	45.0%
	CAAATTGCGGGACGTATCGA+CGG	-	Chr3:48719648-48719667	MsG0380014041.01.T01:CDS	50.0%
	GCTGAACAGGCAGGTACAAA+AGG	-	Chr3:48719615-48719634	MsG0380014041.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	48719393	48719818	48719393	ID=MsG0380014041.01;Name=MsG0380014041.01
Chr3	mRNA	48719393	48719818	48719393	ID=MsG0380014041.01.T01;Parent=MsG0380014041.01;Name=MsG0380014041.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|141
Chr3	exon	48719393	48719818	48719393	ID=MsG0380014041.01.T01:exon:9270;Parent=MsG0380014041.01.T01
Chr3	CDS	48719393	48719818	48719393	ID=MsG0380014041.01.T01:cds;Parent=MsG0380014041.01.T01

Gene Sequence

>MsG0380014041.01.T01

ATGGGACTTCCAACGTTATTAATGGAGGGTAAGCCTCGAAGAATCGAAGAATTACAGACTAATGTGCAAAAAAAACTTAATTGTGTGACCCGAAAAATTAACATTACAATTACAAGAATTCCAAATGCTTATAGCGACCCTAATATTCTTGCAGAATTTATAGCTGGACAATTAAAGAATAGAATTTCGTTTAGGAAAGCAATCAAAAAAGCTATTGAATTAGCTGAACAGGCAGGTACAAAAGGAGTTCAAGTACAAATTGCGGGACGTATCGACGGAAAAGAAATTGCACGTGTCGAATGGATTAGAGAAGGTAGGGTTCCTCTACAAACCATTCGAGCTAAAATTGATTATTGTTCCTATACAGTTCGAACTATTTATGGGGTATTAGGAATCAAAGTTTGGATATTTCTAAACAACGACTAA

Protein sequence

>MsG0380014041.01.T01

MGLPTLLMEGKPRRIEELQTNVQKKLNCVTRKINITITRIPNAYSDPNILAEFIAGQLKNRISFRKAIKKAIELAEQAGTKGVQVQIAGRIDGKEIARVEWIREGRVPLQTIRAKIDYCSYTVRTIYGVLGIKVWIFLNND*