AlfalfaGEDB | MsG0380013920.01

M. sativa cultivar ZhongmuNo.1 / MsG0380013920.01

KEGG: K02950
GO: GO:0003735 GO:0005622 GO:0005840 GO:0006412
PFAM: PF00164

Nr:

Query id	Subject id	identity %	alignment length	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013920.01.T02	QVX30020.1	100	53	1	53	71	123	8.72E-29	108
MsG0380013920.01.T01	AFN68312.1	100	54	6	59	32	85	1.93E-29	109
MsG0380013920.01.T04	AFN68312.1	100	40	1	40	46	85	1.36E-18	80.9
MsG0380013920.01.T03	AFN68312.1	100	45	1	45	41	85	1.17E-21	89

Swissprot:

Query id	Subject id	identity %	alignment length	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013920.01.T01	A4GGC9	100	54	6	59	70	123	3.90E-32	110
MsG0380013920.01.T02	Q9B133	100	53	1	53	71	123	1.28E-31	108
MsG0380013920.01.T03	A4GGC9	100	45	1	45	79	123	2.07E-24	89.7
MsG0380013920.01.T04	A4GGC9	100	40	1	40	84	123	2.96E-21	81.6

Trembl:

Query id	Subject id	identity %	alignment length	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013920.01.T01	A0A6H0DZ74	100.000	54	6	59	70	123	1.35e-29	110
MsG0380013920.01.T01	I6ZSR6	100.000	54	6	59	32	85	9.23e-30	109
MsG0380013920.01.T02	A0A6H0DZ74	100.000	53	1	53	71	123	4.47e-29	108
MsG0380013920.01.T03	I6ZSR6	100.000	45	1	45	41	85	5.61e-22	89.0
MsG0380013920.01.T04	I6ZSR6	100.000	40	1	40	46	85	6.49e-19	80.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048698.01	MsG0380013920.01	0.897883	9.124174e-77	1.052118e-72
MsG0080048699.01	MsG0380013920.01	0.881496	2.287056e-70	1.342999e-66
MsG0180002226.01	MsG0380013920.01	0.903586	2.976721e-79	4.434246e-75
MsG0180002435.01	MsG0380013920.01	0.863477	2.420532e-64	7.347064e-61
MsG0180004434.01	MsG0380013920.01	0.812860	3.428037e-51	2.203799e-48
MsG0180004435.01	MsG0380013920.01	0.821159	4.682705e-53	3.780255e-50
MsG0380013448.01	MsG0380013920.01	0.823606	1.265349e-53	1.094694e-50
MsG0380013915.01	MsG0380013920.01	0.838974	2.089767e-57	2.845169e-54
MsG0380013916.01	MsG0380013920.01	0.924242	9.144532e-90	3.820022e-85
MsG0380013917.01	MsG0380013920.01	0.865079	7.656178e-65	2.459657e-61
MsG0380013918.01	MsG0380013920.01	0.832040	1.185720e-55	1.309881e-52
MsG0380013920.01	MsG0380013923.01	0.884313	2.132939e-71	1.400658e-67
MsG0380013920.01	MsG0380013926.01	0.803289	3.742211e-49	1.873335e-46
MsG0380013920.01	MsG0380013929.01	0.853474	2.333629e-61	5.048400e-58
MsG0380013920.01	MsG0380013931.01	0.880576	4.899653e-70	2.777085e-66
MsG0380013920.01	MsG0380013932.01	0.856584	2.914060e-62	6.989890e-59
MsG0380013920.01	MsG0380013941.01	0.815958	7.080696e-52	4.951705e-49
MsG0380013920.01	MsG0380013942.01	0.895156	1.252690e-75	1.284175e-71
MsG0380013920.01	MsG0380013944.01	0.895537	8.725914e-76	9.092481e-72
MsG0380013920.01	MsG0380013947.01	0.857997	1.114134e-62	2.801977e-59
MsG0380013920.01	MsG0380013948.01	0.930376	1.792153e-93	1.058206e-88
MsG0380013920.01	MsG0380013949.01	0.898795	3.738497e-77	4.491611e-73
MsG0380013920.01	MsG0380013950.01	0.818890	1.548055e-52	1.173394e-49
MsG0380013920.01	MsG0380013951.01	0.869240	3.591277e-66	1.338110e-62
MsG0380013920.01	MsG0380013952.01	0.878176	3.470373e-69	1.794042e-65
MsG0380013920.01	MsG0380013959.01	0.839918	1.188545e-57	1.665950e-54
MsG0380013920.01	MsG0380013962.01	0.802984	4.328197e-49	2.149763e-46
MsG0380013920.01	MsG0380013964.01	0.802812	4.697760e-49	2.323344e-46
MsG0380013920.01	MsG0380013966.01	0.829803	4.196981e-55	4.342164e-52
MsG0380013920.01	MsG0380013968.01	0.865380	6.157229e-65	1.999061e-61
MsG0380013920.01	MsG0380013969.01	0.875289	3.464608e-68	1.609034e-64
MsG0380013920.01	MsG0380013972.01	0.887456	1.404021e-72	1.044885e-68
MsG0380013920.01	MsG0380013981.01	0.879435	1.249383e-69	6.773516e-66
MsG0380013920.01	MsG0380013983.01	0.817958	2.517699e-52	1.859775e-49
MsG0380013920.01	MsG0380013995.01	0.882687	8.451298e-71	5.196893e-67
MsG0380013920.01	MsG0380014008.01	0.900859	4.802204e-78	6.321245e-74
MsG0380013920.01	MsG0380014009.01	0.802063	6.701015e-49	3.251271e-46
MsG0380013920.01	MsG0380014011.01	0.864683	1.019076e-64	3.227486e-61
MsG0380013920.01	MsG0380014018.01	0.874265	7.732089e-68	3.458511e-64
MsG0380013920.01	MsG0380014023.01	0.821732	3.453729e-53	2.833069e-50
MsG0380013920.01	MsG0380014026.01	0.818353	2.049347e-52	1.530518e-49
MsG0380013920.01	MsG0380014030.01	0.828425	9.054821e-55	8.997034e-52
MsG0380013920.01	MsG0380014035.01	0.801386	9.228673e-49	4.401440e-46
MsG0380013920.01	MsG0380014036.01	0.866897	2.037413e-65	6.979502e-62
MsG0380013920.01	MsG0380014039.01	0.872824	2.362713e-67	1.002683e-63
MsG0380013920.01	MsG0380014040.01	0.889922	1.569106e-73	1.291477e-69
MsG0380013920.01	MsG0380014041.01	0.812012	5.251740e-51	3.300843e-48
MsG0380013920.01	MsG0380014044.01	0.879757	9.599152e-70	5.268324e-66
MsG0380013920.01	MsG0380014048.01	0.800879	1.171383e-48	5.515954e-46
MsG0380013920.01	MsG0380014051.01	0.866919	2.004566e-65	6.872175e-62
MsG0380013920.01	MsG0380014055.01	0.802437	5.612232e-49	2.749145e-46
MsG0380013920.01	MsG0380014057.01	0.891470	3.858027e-74	3.387134e-70
MsG0380013920.01	MsG0380015307.01	0.803749	3.005066e-49	1.522225e-46
MsG0380013920.01	MsG0380017006.01	0.815736	7.932824e-52	5.514621e-49
MsG0380013920.01	MsG0380017007.01	0.886372	3.623529e-72	2.580910e-68
MsG0380013920.01	MsG0480018296.01	0.819637	1.046114e-52	8.094869e-50
MsG0380013920.01	MsG0580028230.01	0.807667	4.519725e-50	2.532383e-47
MsG0380013920.01	MsG0680032339.01	0.846822	1.706088e-59	2.973163e-56
MsG0380013920.01	MsG0780038140.01	0.805462	1.319381e-49	6.984795e-47
MsG0380013920.01	MsG0780040316.01	0.830908	2.253138e-55	2.407061e-52
MsG0380013920.01	MsG0780040317.01	0.817643	2.965010e-52	2.171382e-49
MsG0280007042.01	MsG0380013920.01	0.824607	7.366946e-54	6.558008e-51
MsG0280010145.01	MsG0380013920.01	0.894616	2.084965e-75	2.088583e-71

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013920.01.T02	MTR_7g055840	94.340	53	3	1	53	41	93	5.09e-30	100
MsG0380013920.01.T02	MTR_0625s0020	94.340	53	3	1	53	45	97	5.11e-30	100
MsG0380013920.01.T02	MTR_7g055910	88.679	53	6	1	53	143	195	5.30e-27	96.3
MsG0380013920.01.T02	MTR_3g035660	86.275	51	7	1	51	120	170	2.16e-25	96.7
MsG0380013920.01.T02	MTR_0002s0280	93.478	46	3	1	46	112	157	3.47e-25	90.5
MsG0380013920.01.T02	MTR_0082s0040	69.811	53	16	1	53	308	360	1.30e-20	82.4
MsG0380013920.01.T01	MTR_7g055840	94.444	54	3	6	59	40	93	1.65e-30	102
MsG0380013920.01.T01	MTR_0625s0020	94.444	54	3	6	59	44	97	1.83e-30	102
MsG0380013920.01.T01	MTR_7g055910	88.889	54	6	6	59	142	195	1.69e-27	97.8
MsG0380013920.01.T01	MTR_3g035660	86.538	52	7	6	57	119	170	7.19e-26	98.2
MsG0380013920.01.T01	MTR_0002s0280	93.617	47	3	6	52	111	157	7.68e-26	92.4
MsG0380013920.01.T01	MTR_0082s0040	69.811	53	16	7	59	308	360	3.98e-21	84.0
MsG0380013920.01.T04	MTR_7g055840	92.500	40	3	1	40	54	93	7.98e-20	74.3
MsG0380013920.01.T04	MTR_0625s0020	92.500	40	3	1	40	58	97	9.46e-20	74.3
MsG0380013920.01.T04	MTR_7g055910	87.500	40	5	1	40	156	195	9.50e-18	71.6
MsG0380013920.01.T04	MTR_3g035660	81.579	38	7	1	38	133	170	4.51e-16	69.3
MsG0380013920.01.T04	MTR_0002s0280	90.909	33	3	1	33	125	157	4.93e-15	63.9
MsG0380013920.01.T04	MTR_0082s0040	67.500	40	13	1	40	321	360	7.63e-13	60.1
MsG0380013920.01.T03	MTR_0625s0020	93.333	45	3	1	45	53	97	5.46e-23	82.8
MsG0380013920.01.T03	MTR_7g055840	93.333	45	3	1	45	49	93	6.45e-23	82.4
MsG0380013920.01.T03	MTR_7g055910	88.889	45	5	1	45	151	195	6.77e-21	80.1
MsG0380013920.01.T03	MTR_3g035660	83.721	43	7	1	43	128	170	5.00e-19	77.8
MsG0380013920.01.T03	MTR_0002s0280	92.105	38	3	1	38	120	157	5.13e-18	72.0
MsG0380013920.01.T03	MTR_0082s0040	68.182	44	14	2	45	317	360	4.91e-15	66.6

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013920.01.T02	ATCG01230	98.113	53	1	1	53	33	85	6.87e-32	105
MsG0380013920.01.T02	ATCG00905	98.113	53	1	1	53	33	85	6.87e-32	105
MsG0380013920.01.T02	ATMG00980	66.038	53	18	1	53	71	123	5.20e-20	76.6
MsG0380013920.01.T02	AT2G07675	66.038	53	18	1	53	71	123	5.20e-20	76.6
MsG0380013920.01.T01	ATCG01230	98.148	54	1	6	59	32	85	1.34e-32	107
MsG0380013920.01.T01	ATCG00905	98.148	54	1	6	59	32	85	1.34e-32	107
MsG0380013920.01.T01	ATMG00980	66.038	53	18	7	59	71	123	7.29e-20	76.3
MsG0380013920.01.T01	AT2G07675	66.038	53	18	7	59	71	123	7.29e-20	76.3
MsG0380013920.01.T04	ATCG01230	97.500	40	1	1	40	46	85	8.36e-22	79.3
MsG0380013920.01.T04	ATCG00905	97.500	40	1	1	40	46	85	8.36e-22	79.3
MsG0380013920.01.T04	ATMG00980	62.500	40	15	1	40	84	123	2.42e-11	53.9
MsG0380013920.01.T04	AT2G07675	62.500	40	15	1	40	84	123	2.42e-11	53.9
MsG0380013920.01.T03	ATCG01230	97.778	45	1	1	45	41	85	8.90e-25	87.0
MsG0380013920.01.T03	ATCG00905	97.778	45	1	1	45	41	85	8.90e-25	87.0
MsG0380013920.01.T03	ATMG00980	62.222	45	17	1	45	79	123	5.36e-14	60.8
MsG0380013920.01.T03	AT2G07675	62.222	45	17	1	45	79	123	5.36e-14	60.8

Find 21 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGGCTAAAATAATTTATTT+TGG	0.180484	3:+48401872	None:intergenic
CAACAAGGTCGTTCTAAATA+TGG	0.216871	3:-48401905	MsG0380013920.01.T01:CDS
CAGAATGTTCTTGTAAATTA+TGG	0.231614	3:+48402023	None:intergenic
CCTTTACTCCGACAGCATCT+AGG	0.372692	3:+48401933	None:intergenic
CTTGTAAATTATGGCCAATA+CGG	0.377175	3:+48402032	None:intergenic
AACAAGGTCGTTCTAAATAT+GGG	0.408379	3:-48401904	MsG0380013920.01.T01:CDS
CATATTTAGAACGACCTTGT+TGG	0.411865	3:+48401906	None:intergenic
CATTCTGTAGTCTTAGTAAG+AGG	0.441296	3:-48402007	MsG0380013920.01.T01:CDS
CACTGCTTATATACCCGTAT+TGG	0.457936	3:-48402046	MsG0380013920.01.T01:CDS
CTTTACTCCGACAGCATCTA+GGG	0.502834	3:+48401934	None:intergenic
CCTAGATGCTGTCGGAGTAA+AGG	0.506750	3:-48401933	MsG0380013920.01.T01:CDS
TTGTAAATTATGGCCAATAC+GGG	0.513748	3:+48402033	None:intergenic
ATTCTGTAGTCTTAGTAAGA+GGG	0.565369	3:-48402006	MsG0380013920.01.T01:CDS
GTGAGATATCACATTGTTCG+AGG	0.586809	3:-48401959	MsG0380013920.01.T01:CDS
TTCTGTAGTCTTAGTAAGAG+GGG	0.592028	3:-48402005	MsG0380013920.01.T01:CDS
GGAGTAAAGGATCGCCAACA+AGG	0.599055	3:-48401920	MsG0380013920.01.T01:CDS
CGAGGAACCCTAGATGCTGT+CGG	0.617344	3:-48401941	MsG0380013920.01.T01:CDS
ACAAGGTCGTTCTAAATATG+GGG	0.627597	3:-48401903	MsG0380013920.01.T01:CDS
GGAAGGGTTAAGGATTTACC+TGG	0.642987	3:-48401983	MsG0380013920.01.T01:CDS
TCTGTAGTCTTAGTAAGAGG+GGG	0.664205	3:-48402004	MsG0380013920.01.T01:CDS
ACAATGTGATATCTCACACC+AGG	0.678462	3:+48401965	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CAGAATGTTCTTGTAAATTA+TGG	+	Chr3:48401899-48401918	None:intergenic	25.0%
	CTTGTAAATTATGGCCAATA+CGG	+	Chr3:48401890-48401909	None:intergenic	30.0%
	TTGTAAATTATGGCCAATAC+GGG	+	Chr3:48401889-48401908	None:intergenic	30.0%
!	AACAAGGTCGTTCTAAATAT+GGG	-	Chr3:48402015-48402034	MsG0380013920.01.T01:CDS	30.0%
!	ATTCTGTAGTCTTAGTAAGA+GGG	-	Chr3:48401913-48401932	MsG0380013920.01.T01:CDS	30.0%
!	ACAAGGTCGTTCTAAATATG+GGG	-	Chr3:48402016-48402035	MsG0380013920.01.T01:CDS	35.0%
!	CAACAAGGTCGTTCTAAATA+TGG	-	Chr3:48402014-48402033	MsG0380013920.01.T01:CDS	35.0%
!	CATATTTAGAACGACCTTGT+TGG	+	Chr3:48402016-48402035	None:intergenic	35.0%
!	CATTCTGTAGTCTTAGTAAG+AGG	-	Chr3:48401912-48401931	MsG0380013920.01.T01:CDS	35.0%
!	TTCTGTAGTCTTAGTAAGAG+GGG	-	Chr3:48401914-48401933	MsG0380013920.01.T01:CDS	35.0%
	ACAATGTGATATCTCACACC+AGG	+	Chr3:48401957-48401976	None:intergenic	40.0%
	GTGAGATATCACATTGTTCG+AGG	-	Chr3:48401960-48401979	MsG0380013920.01.T01:CDS	40.0%
!	TCTGTAGTCTTAGTAAGAGG+GGG	-	Chr3:48401915-48401934	MsG0380013920.01.T01:CDS	40.0%
	AGTCTTAGTAAGAGGGGGAA+GGG	-	Chr3:48401920-48401939	MsG0380013920.01.T01:CDS	45.0%
	CTTTACTCCGACAGCATCTA+GGG	+	Chr3:48401988-48402007	None:intergenic	45.0%
	GGAAGGGTTAAGGATTTACC+TGG	-	Chr3:48401936-48401955	MsG0380013920.01.T01:CDS	45.0%
!	TAGTCTTAGTAAGAGGGGGA+AGG	-	Chr3:48401919-48401938	MsG0380013920.01.T01:CDS	45.0%
	AGTAAGAGGGGGAAGGGTTA+AGG	-	Chr3:48401926-48401945	MsG0380013920.01.T01:CDS	50.0%
	CCTAGATGCTGTCGGAGTAA+AGG	-	Chr3:48401986-48402005	MsG0380013920.01.T01:CDS	50.0%
	CCTTTACTCCGACAGCATCT+AGG	+	Chr3:48401989-48402008	None:intergenic	50.0%
	GGAGTAAAGGATCGCCAACA+AGG	-	Chr3:48401999-48402018	MsG0380013920.01.T01:CDS	50.0%
	CGAGGAACCCTAGATGCTGT+CGG	-	Chr3:48401978-48401997	MsG0380013920.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	48401876	48402065	48401876	ID=MsG0380013920.01;Name=MsG0380013920.01
Chr3	mRNA	48401886	48402065	48401886	ID=MsG0380013920.01.T01;Parent=MsG0380013920.01;Name=MsG0380013920.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|59
Chr3	exon	48401886	48402065	48401886	ID=MsG0380013920.01.T01:exon:9432;Parent=MsG0380013920.01.T01
Chr3	CDS	48401886	48402065	48401886	ID=MsG0380013920.01.T01:cds;Parent=MsG0380013920.01.T01
Chr3	mRNA	48401886	48402047	48401886	ID=MsG0380013920.01.T02;Parent=MsG0380013920.01;Name=MsG0380013920.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|53
Chr3	exon	48401886	48402047	48401886	ID=MsG0380013920.01.T02:exon:9433;Parent=MsG0380013920.01.T02
Chr3	CDS	48401886	48402047	48401886	ID=MsG0380013920.01.T02:cds;Parent=MsG0380013920.01.T02
Chr3	mRNA	48401886	48402023	48401886	ID=MsG0380013920.01.T03;Parent=MsG0380013920.01;Name=MsG0380013920.01.T03;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|45
Chr3	exon	48401886	48402023	48401886	ID=MsG0380013920.01.T03:exon:9434;Parent=MsG0380013920.01.T03
Chr3	CDS	48401886	48402023	48401886	ID=MsG0380013920.01.T03:cds;Parent=MsG0380013920.01.T03
Chr3	mRNA	48401886	48402008	48401886	ID=MsG0380013920.01.T04;Parent=MsG0380013920.01;Name=MsG0380013920.01.T04;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|40
Chr3	exon	48401886	48402008	48401886	ID=MsG0380013920.01.T04:exon:9435;Parent=MsG0380013920.01.T04
Chr3	CDS	48401886	48402008	48401886	ID=MsG0380013920.01.T04:cds;Parent=MsG0380013920.01.T04
Chr3	mRNA	48401876	48401908	48401876	ID=MsG0380013920.01.T05;Parent=MsG0380013920.01;Name=MsG0380013920.01.T05;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|10
Chr3	exon	48401876	48401908	48401876	ID=MsG0380013920.01.T05:exon:9436;Parent=MsG0380013920.01.T05
Chr3	CDS	48401876	48401908	48401876	ID=MsG0380013920.01.T05:cds;Parent=MsG0380013920.01.T05

Gene Sequence

>MsG0380013920.01.T02

GGCCATAATTTACAAGAACATTCTGTAGTCTTAGTAAGAGGGGGAAGGGTTAAGGATTTACCTGGTGTGAGATATCACATTGTTCGAGGAACCCTAGATGCTGTCGGAGTAAAGGATCGCCAACAAGGTCGTTCTAAATATGGGGCAAAAAAGCCAAAATAA

>MsG0380013920.01.T01

TGCTTATATACCCGTATTGGCCATAATTTACAAGAACATTCTGTAGTCTTAGTAAGAGGGGGAAGGGTTAAGGATTTACCTGGTGTGAGATATCACATTGTTCGAGGAACCCTAGATGCTGTCGGAGTAAAGGATCGCCAACAAGGTCGTTCTAAATATGGGGCAAAAAAGCCAAAATAA

>MsG0380013920.01.T04

GGGGGAAGGGTTAAGGATTTACCTGGTGTGAGATATCACATTGTTCGAGGAACCCTAGATGCTGTCGGAGTAAAGGATCGCCAACAAGGTCGTTCTAAATATGGGGCAAAAAAGCCAAAATAA

>MsG0380013920.01.T05

ATGGGGCAAAAAAGCCAAAATAAATTATTTTAG

>MsG0380013920.01.T03

GTAGTCTTAGTAAGAGGGGGAAGGGTTAAGGATTTACCTGGTGTGAGATATCACATTGTTCGAGGAACCCTAGATGCTGTCGGAGTAAAGGATCGCCAACAAGGTCGTTCTAAATATGGGGCAAAAAAGCCAAAATAA

Protein sequence

>MsG0380013920.01.T02

GHNLQEHSVVLVRGGRVKDLPGVRYHIVRGTLDAVGVKDRQQGRSKYGAKKPK*

>MsG0380013920.01.T01

CLYTRIGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGTLDAVGVKDRQQGRSKYGAKKPK*

>MsG0380013920.01.T04

GGRVKDLPGVRYHIVRGTLDAVGVKDRQQGRSKYGAKKPK*

>MsG0380013920.01.T05

MGQKSQNKLF*

>MsG0380013920.01.T03

VVLVRGGRVKDLPGVRYHIVRGTLDAVGVKDRQQGRSKYGAKKPK*