Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014009.01.T01 | YP_009141647.1 | 100 | 141 | 0 | 0 | 1 | 141 | 74 | 214 | 8.82E-96 | 285 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014009.01.T01 | Q9BBP8 | 87.943 | 141 | 17 | 0 | 1 | 141 | 78 | 218 | 1.09E-86 | 254 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014009.01.T01 | A0A0F6NLE8 | 100.000 | 141 | 0 | 0 | 1 | 141 | 74 | 214 | 4.21e-96 | 285 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048689.01 | MsG0380014009.01 | 0.837244 | 5.826293e-57 | 7.522628e-54 |
| MsG0080049079.01 | MsG0380014009.01 | 0.824421 | 8.147632e-54 | 7.212816e-51 |
| MsG0080049080.01 | MsG0380014009.01 | 0.818938 | 1.509524e-52 | 1.145795e-49 |
| MsG0180002226.01 | MsG0380014009.01 | 0.804190 | 2.432939e-49 | 1.246431e-46 |
| MsG0180002435.01 | MsG0380014009.01 | 0.837867 | 4.034658e-57 | 5.310754e-54 |
| MsG0380013889.01 | MsG0380014009.01 | 0.801721 | 7.877772e-49 | 3.789009e-46 |
| MsG0380013916.01 | MsG0380014009.01 | 0.808530 | 2.960852e-50 | 1.697011e-47 |
| MsG0380013917.01 | MsG0380014009.01 | 0.836762 | 7.735830e-57 | 9.844666e-54 |
| MsG0380013918.01 | MsG0380014009.01 | 0.841529 | 4.497793e-58 | 6.627544e-55 |
| MsG0380013920.01 | MsG0380014009.01 | 0.802063 | 6.701015e-49 | 3.251271e-46 |
| MsG0380013930.01 | MsG0380014009.01 | 0.815853 | 7.470262e-52 | 5.209054e-49 |
| MsG0380013931.01 | MsG0380014009.01 | 0.801262 | 9.784158e-49 | 4.651879e-46 |
| MsG0380013932.01 | MsG0380014009.01 | 0.865013 | 8.032903e-65 | 2.574821e-61 |
| MsG0380013939.01 | MsG0380014009.01 | 0.808792 | 2.602025e-50 | 1.501562e-47 |
| MsG0380013944.01 | MsG0380014009.01 | 0.809221 | 2.105925e-50 | 1.229358e-47 |
| MsG0380013948.01 | MsG0380014009.01 | 0.805212 | 1.488306e-49 | 7.828419e-47 |
| MsG0380013949.01 | MsG0380014009.01 | 0.827916 | 1.201082e-54 | 1.175773e-51 |
| MsG0380013950.01 | MsG0380014009.01 | 0.823535 | 1.314787e-53 | 1.135141e-50 |
| MsG0380013952.01 | MsG0380014009.01 | 0.819674 | 1.025783e-52 | 7.945823e-50 |
| MsG0380013958.01 | MsG0380014009.01 | 0.835213 | 1.912772e-56 | 2.323478e-53 |
| MsG0380013959.01 | MsG0380014009.01 | 0.858161 | 9.959543e-63 | 2.518348e-59 |
| MsG0380013964.01 | MsG0380014009.01 | 0.848198 | 7.140722e-60 | 1.300150e-56 |
| MsG0380013965.01 | MsG0380014009.01 | 0.804602 | 1.996450e-49 | 1.033740e-46 |
| MsG0380013969.01 | MsG0380014009.01 | 0.815578 | 8.604423e-52 | 5.955192e-49 |
| MsG0380013972.01 | MsG0380014009.01 | 0.848481 | 5.965278e-60 | 1.096372e-56 |
| MsG0380013978.01 | MsG0380014009.01 | 0.802464 | 5.540787e-49 | 2.715976e-46 |
| MsG0380013979.01 | MsG0380014009.01 | 0.818392 | 2.007234e-52 | 1.500685e-49 |
| MsG0380013980.01 | MsG0380014009.01 | 0.845646 | 3.567600e-59 | 5.985702e-56 |
| MsG0380013981.01 | MsG0380014009.01 | 0.864797 | 9.385436e-65 | 2.984321e-61 |
| MsG0380013983.01 | MsG0380014009.01 | 0.829109 | 6.187111e-55 | 6.271311e-52 |
| MsG0380013988.01 | MsG0380014009.01 | 0.944032 | 4.097072e-103 | 5.732254e-98 |
| MsG0380013995.01 | MsG0380014009.01 | 0.859445 | 4.117104e-63 | 1.087635e-59 |
| MsG0380013996.01 | MsG0380014009.01 | 0.808854 | 2.523929e-50 | 1.458896e-47 |
| MsG0380014008.01 | MsG0380014009.01 | 0.844297 | 8.250919e-59 | 1.326134e-55 |
| MsG0380014009.01 | MsG0380014010.01 | 0.821373 | 4.179009e-53 | 3.393676e-50 |
| MsG0380014009.01 | MsG0380014011.01 | 0.839838 | 1.246721e-57 | 1.742991e-54 |
| MsG0380014009.01 | MsG0380014013.01 | 0.812453 | 4.208233e-51 | 2.676915e-48 |
| MsG0380014009.01 | MsG0380014030.01 | 0.805310 | 1.420005e-49 | 7.487872e-47 |
| MsG0380014009.01 | MsG0380014034.01 | 0.810887 | 9.218635e-51 | 5.624186e-48 |
| MsG0380014009.01 | MsG0380014035.01 | 0.841317 | 5.113444e-58 | 7.484535e-55 |
| MsG0380014009.01 | MsG0380014036.01 | 0.860705 | 1.714480e-63 | 4.729953e-60 |
| MsG0380014009.01 | MsG0380014037.01 | 0.832519 | 9.027537e-56 | 1.011429e-52 |
| MsG0380014009.01 | MsG0380014038.01 | 0.802228 | 6.196856e-49 | 3.019302e-46 |
| MsG0380014009.01 | MsG0380014040.01 | 0.826901 | 2.103144e-54 | 1.998870e-51 |
| MsG0380014009.01 | MsG0380014041.01 | 0.995603 | 6.033759e-218 | 8.917207e-211 |
| MsG0380014009.01 | MsG0380014042.01 | 0.830668 | 2.579755e-55 | 2.736433e-52 |
| MsG0380014009.01 | MsG0380014044.01 | 0.857970 | 1.135385e-62 | 2.852832e-59 |
| MsG0380014009.01 | MsG0380014052.01 | 0.811474 | 6.874659e-51 | 4.259631e-48 |
| MsG0380014009.01 | MsG0380014055.01 | 0.994602 | 1.299804e-208 | 1.472738e-201 |
| MsG0380014009.01 | MsG0380014056.01 | 0.861939 | 7.212315e-64 | 2.075469e-60 |
| MsG0380014009.01 | MsG0380014057.01 | 0.813763 | 2.170629e-51 | 1.430031e-48 |
| MsG0380014009.01 | MsG0380017006.01 | 0.808978 | 2.374278e-50 | 1.376940e-47 |
| MsG0380014009.01 | MsG0580028230.01 | 0.801732 | 7.837297e-49 | 3.770596e-46 |
| MsG0380014009.01 | MsG0680033966.01 | 0.801871 | 7.339853e-49 | 3.543999e-46 |
| MsG0380014009.01 | MsG0780040316.01 | 0.840205 | 1.000475e-57 | 1.414924e-54 |
| MsG0380014009.01 | MsG0780040317.01 | 0.820938 | 5.264041e-53 | 4.223685e-50 |
| MsG0380014009.01 | MsG0880043541.01 | 0.821148 | 4.709428e-53 | 3.800658e-50 |
| MsG0380014009.01 | MsG0880044440.01 | 0.828220 | 1.014775e-54 | 1.002263e-51 |
| MsG0280007041.01 | MsG0380014009.01 | 0.822010 | 2.977069e-53 | 2.461453e-50 |
| MsG0280007042.01 | MsG0380014009.01 | 0.866911 | 2.016612e-65 | 6.911451e-62 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014009.01.T01 | MTR_4g051410 | 97.163 | 141 | 4 | 0 | 1 | 141 | 74 | 214 | 2.19e-95 | 274 |
| MsG0380014009.01.T01 | MTR_8g038470 | 79.310 | 116 | 17 | 1 | 13 | 128 | 63 | 171 | 1.04e-56 | 176 |
| MsG0380014009.01.T01 | MTR_0002s0790 | 59.574 | 141 | 55 | 1 | 1 | 141 | 101 | 239 | 2.10e-56 | 176 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014009.01.T01 | ATCG00800 | 82.979 | 141 | 24 | 0 | 1 | 141 | 78 | 218 | 2.62e-84 | 246 |
Find 27 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATACAGTTCGAACTATTTA+TGG | 0.120735 | 3:-48640224 | MsG0380014009.01.T01:CDS |
| ATAAATTCTGCAAGAATATT+AGG | 0.153378 | 3:+48640446 | None:intergenic |
| CGAACTATTTATGGGGTATT+AGG | 0.214307 | 3:-48640215 | MsG0380014009.01.T01:CDS |
| GGGACTTCCAACGTTATTAA+TGG | 0.264579 | 3:-48640582 | MsG0380014009.01.T01:CDS |
| TAAAGAATAGAATTTCGTTT+AGG | 0.349086 | 3:-48640412 | MsG0380014009.01.T01:CDS |
| AGCTATTGAATTAGCTGAAC+AGG | 0.363850 | 3:-48640375 | MsG0380014009.01.T01:CDS |
| ATACAGTTCGAACTATTTAT+GGG | 0.369096 | 3:-48640223 | MsG0380014009.01.T01:CDS |
| GGGTATTAGGAATCAAAGTT+TGG | 0.387551 | 3:-48640202 | MsG0380014009.01.T01:CDS |
| TTAGGGTCGCTATAAGCATT+TGG | 0.411303 | 3:+48640464 | None:intergenic |
| TAAATTCTGCAAGAATATTA+GGG | 0.426926 | 3:+48640447 | None:intergenic |
| ATAAATAGTTCGAACTGTAT+AGG | 0.427068 | 3:+48640226 | None:intergenic |
| GGAGTTCAAGTACAAATTGC+GGG | 0.442137 | 3:-48640341 | MsG0380014009.01.T01:CDS |
| TGTAATTCTTCGATTCTTCG+AGG | 0.463402 | 3:+48640551 | None:intergenic |
| ATTCTTGCAGAATTTATAGC+TGG | 0.468432 | 3:-48640440 | MsG0380014009.01.T01:CDS |
| AAGAAATTGCACGTGTCGAA+TGG | 0.483791 | 3:-48640304 | MsG0380014009.01.T01:CDS |
| GCTGAACAGGCAGGTACAAA+AGG | 0.497837 | 3:-48640362 | MsG0380014009.01.T01:CDS |
| CGAATGGATTAGAGAAGGTA+GGG | 0.541012 | 3:-48640288 | MsG0380014009.01.T01:CDS |
| CAAATTGCGGGACGTATCGA+CGG | 0.567491 | 3:-48640329 | MsG0380014009.01.T01:CDS |
| TTAGCTCGAATGGTTTGTAG+AGG | 0.585057 | 3:+48640263 | None:intergenic |
| ACTTCCAACGTTATTAATGG+AGG | 0.585517 | 3:-48640579 | MsG0380014009.01.T01:CDS |
| TCGAATGGATTAGAGAAGGT+AGG | 0.595160 | 3:-48640289 | MsG0380014009.01.T01:CDS |
| CTTCCAACGTTATTAATGGA+GGG | 0.595517 | 3:-48640578 | MsG0380014009.01.T01:CDS |
| ATTGAATTAGCTGAACAGGC+AGG | 0.608696 | 3:-48640371 | MsG0380014009.01.T01:CDS |
| TTACCCTCCATTAATAACGT+TGG | 0.614669 | 3:+48640575 | None:intergenic |
| CGTGTCGAATGGATTAGAGA+AGG | 0.634314 | 3:-48640293 | MsG0380014009.01.T01:CDS |
| TACAGTTCGAACTATTTATG+GGG | 0.637128 | 3:-48640222 | MsG0380014009.01.T01:CDS |
| AGGAGTTCAAGTACAAATTG+CGG | 0.647406 | 3:-48640342 | MsG0380014009.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATTGTAATGTTAATTTTT+CGG | + | Chr3:48640273-48640292 | None:intergenic | 10.0% |
| !!! | AATTGTAATGTTAATTTTTC+GGG | + | Chr3:48640272-48640291 | None:intergenic | 15.0% |
| !! | ATAAATTCTGCAAGAATATT+AGG | + | Chr3:48640322-48640341 | None:intergenic | 20.0% |
| !! | TAAAGAATAGAATTTCGTTT+AGG | - | Chr3:48640353-48640372 | MsG0380014009.01.T01:CDS | 20.0% |
| !! | TAAATTCTGCAAGAATATTA+GGG | + | Chr3:48640321-48640340 | None:intergenic | 20.0% |
| ! | ATAAATAGTTCGAACTGTAT+AGG | + | Chr3:48640542-48640561 | None:intergenic | 25.0% |
| ! | ATACAGTTCGAACTATTTAT+GGG | - | Chr3:48640542-48640561 | MsG0380014009.01.T01:CDS | 25.0% |
| ! | TATACAGTTCGAACTATTTA+TGG | - | Chr3:48640541-48640560 | MsG0380014009.01.T01:CDS | 25.0% |
| !!! | ATAATCAATTTTAGCTCGAA+TGG | + | Chr3:48640515-48640534 | None:intergenic | 25.0% |
| ATTCTTGCAGAATTTATAGC+TGG | - | Chr3:48640325-48640344 | MsG0380014009.01.T01:CDS | 30.0% | |
| TACAGTTCGAACTATTTATG+GGG | - | Chr3:48640543-48640562 | MsG0380014009.01.T01:CDS | 30.0% | |
| ACTTCCAACGTTATTAATGG+AGG | - | Chr3:48640186-48640205 | MsG0380014009.01.T01:CDS | 35.0% | |
| AGCTATTGAATTAGCTGAAC+AGG | - | Chr3:48640390-48640409 | MsG0380014009.01.T01:CDS | 35.0% | |
| AGGAGTTCAAGTACAAATTG+CGG | - | Chr3:48640423-48640442 | MsG0380014009.01.T01:CDS | 35.0% | |
| CTTCCAACGTTATTAATGGA+GGG | - | Chr3:48640187-48640206 | MsG0380014009.01.T01:CDS | 35.0% | |
| GGGTATTAGGAATCAAAGTT+TGG | - | Chr3:48640563-48640582 | MsG0380014009.01.T01:CDS | 35.0% | |
| TGTAATTCTTCGATTCTTCG+AGG | + | Chr3:48640217-48640236 | None:intergenic | 35.0% | |
| TTACCCTCCATTAATAACGT+TGG | + | Chr3:48640193-48640212 | None:intergenic | 35.0% | |
| ! | CGAACTATTTATGGGGTATT+AGG | - | Chr3:48640550-48640569 | MsG0380014009.01.T01:CDS | 35.0% |
| AAGAAATTGCACGTGTCGAA+TGG | - | Chr3:48640461-48640480 | MsG0380014009.01.T01:CDS | 40.0% | |
| ATTGAATTAGCTGAACAGGC+AGG | - | Chr3:48640394-48640413 | MsG0380014009.01.T01:CDS | 40.0% | |
| GGAGTTCAAGTACAAATTGC+GGG | - | Chr3:48640424-48640443 | MsG0380014009.01.T01:CDS | 40.0% | |
| GGGACTTCCAACGTTATTAA+TGG | - | Chr3:48640183-48640202 | MsG0380014009.01.T01:CDS | 40.0% | |
| TTAGGGTCGCTATAAGCATT+TGG | + | Chr3:48640304-48640323 | None:intergenic | 40.0% | |
| ! | TTAGCTCGAATGGTTTGTAG+AGG | + | Chr3:48640505-48640524 | None:intergenic | 40.0% |
| !! | CGAATGGATTAGAGAAGGTA+GGG | - | Chr3:48640477-48640496 | MsG0380014009.01.T01:CDS | 40.0% |
| !! | TCGAATGGATTAGAGAAGGT+AGG | - | Chr3:48640476-48640495 | MsG0380014009.01.T01:CDS | 40.0% |
| !! | CGTGTCGAATGGATTAGAGA+AGG | - | Chr3:48640472-48640491 | MsG0380014009.01.T01:CDS | 45.0% |
| CAAATTGCGGGACGTATCGA+CGG | - | Chr3:48640436-48640455 | MsG0380014009.01.T01:CDS | 50.0% | |
| GCTGAACAGGCAGGTACAAA+AGG | - | Chr3:48640403-48640422 | MsG0380014009.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 48640181 | 48640606 | 48640181 | ID=MsG0380014009.01;Name=MsG0380014009.01 |
| Chr3 | mRNA | 48640181 | 48640606 | 48640181 | ID=MsG0380014009.01.T01;Parent=MsG0380014009.01;Name=MsG0380014009.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|141 |
| Chr3 | exon | 48640181 | 48640606 | 48640181 | ID=MsG0380014009.01.T01:exon:56994;Parent=MsG0380014009.01.T01 |
| Chr3 | CDS | 48640181 | 48640606 | 48640181 | ID=MsG0380014009.01.T01:cds;Parent=MsG0380014009.01.T01 |
| Gene Sequence |
| Protein sequence |
