Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013948.01.T01 | AWW91837.1 | 100 | 116 | 0 | 0 | 1 | 116 | 168 | 283 | 8.33E-79 | 243 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013948.01.T01 | P0CC48 | 95.327 | 107 | 5 | 0 | 1 | 107 | 186 | 292 | 4.14E-65 | 208 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013948.01.T01 | A0A2Z4KH50 | 100.000 | 116 | 0 | 0 | 1 | 116 | 168 | 283 | 3.98e-79 | 243 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048692.01 | MsG0380013948.01 | 0.809980 | 1.446857e-50 | 8.617712e-48 |
| MsG0080048698.01 | MsG0380013948.01 | 0.898883 | 3.427642e-77 | 4.132376e-73 |
| MsG0080048699.01 | MsG0380013948.01 | 0.871314 | 7.510835e-67 | 3.016401e-63 |
| MsG0180002226.01 | MsG0380013948.01 | 0.874258 | 7.770243e-68 | 3.474502e-64 |
| MsG0180002435.01 | MsG0380013948.01 | 0.858588 | 7.430874e-63 | 1.905961e-59 |
| MsG0180003624.01 | MsG0380013948.01 | 0.802896 | 4.512798e-49 | 2.236577e-46 |
| MsG0180004434.01 | MsG0380013948.01 | 0.837833 | 4.116092e-57 | 5.412547e-54 |
| MsG0180004435.01 | MsG0380013948.01 | 0.846721 | 1.817554e-59 | 3.157400e-56 |
| MsG0380013448.01 | MsG0380013948.01 | 0.836719 | 7.937087e-57 | 1.008698e-53 |
| MsG0380013888.01 | MsG0380013948.01 | 0.808519 | 2.976596e-50 | 1.705554e-47 |
| MsG0380013889.01 | MsG0380013948.01 | 0.801851 | 7.407298e-49 | 3.574734e-46 |
| MsG0380013915.01 | MsG0380013948.01 | 0.838203 | 3.306980e-57 | 4.397471e-54 |
| MsG0380013916.01 | MsG0380013948.01 | 0.969500 | 3.250573e-130 | 3.010666e-124 |
| MsG0380013917.01 | MsG0380013948.01 | 0.877274 | 7.165786e-69 | 3.582063e-65 |
| MsG0380013918.01 | MsG0380013948.01 | 0.849134 | 3.928267e-60 | 7.375603e-57 |
| MsG0380013920.01 | MsG0380013948.01 | 0.930376 | 1.792153e-93 | 1.058206e-88 |
| MsG0380013923.01 | MsG0380013948.01 | 0.868793 | 5.011991e-66 | 1.836645e-62 |
| MsG0380013926.01 | MsG0380013948.01 | 0.823087 | 1.672989e-53 | 1.426390e-50 |
| MsG0380013929.01 | MsG0380013948.01 | 0.845593 | 3.686145e-59 | 6.173368e-56 |
| MsG0380013931.01 | MsG0380013948.01 | 0.884552 | 1.739327e-71 | 1.152613e-67 |
| MsG0380013932.01 | MsG0380013948.01 | 0.859774 | 3.277349e-63 | 8.756437e-60 |
| MsG0380013941.01 | MsG0380013948.01 | 0.816148 | 6.420241e-52 | 4.513405e-49 |
| MsG0380013942.01 | MsG0380013948.01 | 0.885998 | 5.012518e-72 | 3.515929e-68 |
| MsG0380013944.01 | MsG0380013948.01 | 0.877866 | 4.455082e-69 | 2.276216e-65 |
| MsG0380013947.01 | MsG0380013948.01 | 0.871087 | 8.922813e-67 | 3.553546e-63 |
| MsG0380013948.01 | MsG0380013949.01 | 0.874375 | 7.093829e-68 | 3.185639e-64 |
| MsG0380013948.01 | MsG0380013951.01 | 0.863333 | 2.682826e-64 | 8.104203e-61 |
| MsG0380013948.01 | MsG0380013952.01 | 0.886118 | 4.514579e-72 | 3.183089e-68 |
| MsG0380013948.01 | MsG0380013959.01 | 0.838766 | 2.366179e-57 | 3.201137e-54 |
| MsG0380013948.01 | MsG0380013962.01 | 0.817963 | 2.511180e-52 | 1.855207e-49 |
| MsG0380013948.01 | MsG0380013964.01 | 0.821883 | 3.185301e-53 | 2.624320e-50 |
| MsG0380013948.01 | MsG0380013966.01 | 0.838151 | 3.410186e-57 | 4.527102e-54 |
| MsG0380013948.01 | MsG0380013968.01 | 0.859387 | 4.284777e-63 | 1.129748e-59 |
| MsG0380013948.01 | MsG0380013969.01 | 0.883026 | 6.354207e-71 | 3.961597e-67 |
| MsG0380013948.01 | MsG0380013972.01 | 0.891473 | 3.847819e-74 | 3.378521e-70 |
| MsG0380013948.01 | MsG0380013981.01 | 0.890560 | 8.819359e-74 | 7.454949e-70 |
| MsG0380013948.01 | MsG0380013983.01 | 0.838479 | 2.807317e-57 | 3.764758e-54 |
| MsG0380013948.01 | MsG0380013995.01 | 0.872561 | 2.894284e-67 | 1.216777e-63 |
| MsG0380013948.01 | MsG0380014008.01 | 0.876087 | 1.845278e-68 | 8.829077e-65 |
| MsG0380013948.01 | MsG0380014009.01 | 0.805212 | 1.488306e-49 | 7.828419e-47 |
| MsG0380013948.01 | MsG0380014011.01 | 0.837171 | 6.082731e-57 | 7.836850e-54 |
| MsG0380013948.01 | MsG0380014018.01 | 0.863623 | 2.181729e-64 | 6.656909e-61 |
| MsG0380013948.01 | MsG0380014023.01 | 0.850348 | 1.800204e-60 | 3.515707e-57 |
| MsG0380013948.01 | MsG0380014026.01 | 0.826630 | 2.441995e-54 | 2.302644e-51 |
| MsG0380013948.01 | MsG0380014030.01 | 0.806620 | 7.528204e-50 | 4.104990e-47 |
| MsG0380013948.01 | MsG0380014033.01 | 0.811574 | 6.540632e-51 | 4.063237e-48 |
| MsG0380013948.01 | MsG0380014034.01 | 0.828035 | 1.124488e-54 | 1.104697e-51 |
| MsG0380013948.01 | MsG0380014035.01 | 0.818224 | 2.191040e-52 | 1.630385e-49 |
| MsG0380013948.01 | MsG0380014036.01 | 0.864849 | 9.044379e-65 | 2.880630e-61 |
| MsG0380013948.01 | MsG0380014039.01 | 0.863798 | 1.924213e-64 | 5.907765e-61 |
| MsG0380013948.01 | MsG0380014040.01 | 0.872932 | 2.173982e-67 | 9.261150e-64 |
| MsG0380013948.01 | MsG0380014041.01 | 0.811433 | 7.017855e-51 | 4.343654e-48 |
| MsG0380013948.01 | MsG0380014044.01 | 0.843814 | 1.111600e-58 | 1.759803e-55 |
| MsG0380013948.01 | MsG0380014051.01 | 0.854087 | 1.554793e-61 | 3.433003e-58 |
| MsG0380013948.01 | MsG0380014055.01 | 0.804237 | 2.379220e-49 | 1.220346e-46 |
| MsG0380013948.01 | MsG0380014057.01 | 0.872761 | 2.480493e-67 | 1.050358e-63 |
| MsG0380013948.01 | MsG0380017007.01 | 0.866131 | 3.568329e-65 | 1.189812e-61 |
| MsG0380013948.01 | MsG0480018296.01 | 0.836767 | 7.717435e-57 | 9.822597e-54 |
| MsG0380013948.01 | MsG0580027677.01 | 0.801357 | 9.355681e-49 | 4.458852e-46 |
| MsG0380013948.01 | MsG0580028230.01 | 0.805547 | 1.266299e-49 | 6.718050e-47 |
| MsG0380013948.01 | MsG0680032339.01 | 0.836596 | 8.527405e-57 | 1.079699e-53 |
| MsG0380013948.01 | MsG0780040316.01 | 0.853619 | 2.120054e-61 | 4.608807e-58 |
| MsG0380013948.01 | MsG0780040317.01 | 0.836149 | 1.108323e-56 | 1.384681e-53 |
| MsG0280007042.01 | MsG0380013948.01 | 0.831382 | 1.723364e-55 | 1.867079e-52 |
| MsG0280010145.01 | MsG0380013948.01 | 0.890484 | 9.447403e-74 | 7.956449e-70 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013948.01.T01 | MTR_1g088510 | 99.083 | 109 | 1 | 0 | 1 | 109 | 63 | 171 | 5.71e-76 | 222 |
| MsG0380013948.01.T01 | MTR_3g035660 | 94.495 | 109 | 6 | 0 | 1 | 109 | 490 | 598 | 2.29e-66 | 214 |
| MsG0380013948.01.T01 | MTR_0002s0310 | 92.135 | 89 | 7 | 0 | 1 | 89 | 63 | 151 | 1.97e-50 | 164 |
| MsG0380013948.01.T01 | MTR_1g019230 | 88.158 | 76 | 9 | 0 | 1 | 76 | 6 | 81 | 6.83e-43 | 135 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013948.01.T01 | ATCG01250 | 100.000 | 74 | 0 | 0 | 1 | 74 | 63 | 136 | 8.56e-46 | 152 |
| MsG0380013948.01.T01 | ATCG00890 | 100.000 | 74 | 0 | 0 | 1 | 74 | 63 | 136 | 8.56e-46 | 152 |
Find 36 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATGGTTTCTCTTGGCTATA+TGG | 0.197078 | 3:-48492635 | MsG0380013948.01.T01:CDS |
| TGGGGCAAGCTCTTCTATTC+TGG | 0.197255 | 3:-48492660 | MsG0380013948.01.T01:CDS |
| CTATCTATTTCTGAGATGTT+TGG | 0.246287 | 3:-48492413 | MsG0380013948.01.T01:CDS |
| ATATTCATCACTGTAGGAAT+TGG | 0.280413 | 3:-48492521 | MsG0380013948.01.T01:CDS |
| AGCTCTTCTATTCTGGTTCA+TGG | 0.287942 | 3:-48492653 | MsG0380013948.01.T01:CDS |
| TTCTGGTTCATGGTTTCTCT+TGG | 0.336946 | 3:-48492643 | MsG0380013948.01.T01:CDS |
| CCCTAGCCCCTTCTCATCAA+TGG | 0.393307 | 3:-48492487 | MsG0380013948.01.T01:CDS |
| TATTCATCACTGTAGGAATT+GGG | 0.417349 | 3:-48492520 | MsG0380013948.01.T01:CDS |
| TCTTGGCTATATGGTTCGTC+CGG | 0.418558 | 3:-48492626 | MsG0380013948.01.T01:CDS |
| CTTGGCTATATGGTTCGTCC+GGG | 0.468938 | 3:-48492625 | MsG0380013948.01.T01:CDS |
| TAAAATATTTACTCATGGGT+GGG | 0.477998 | 3:-48492679 | None:intergenic |
| TAACAAGTAAAGTTATGTCT+TGG | 0.481945 | 3:+48492338 | None:intergenic |
| ATAAGCGCAATTGAAATTCC+AGG | 0.496050 | 3:+48492542 | None:intergenic |
| CGCACTCCTTCATATACGTC+AGG | 0.510042 | 3:+48492461 | None:intergenic |
| TCCATTGATGAGAAGGGGCT+AGG | 0.510524 | 3:+48492486 | None:intergenic |
| TTGAAGCTCTATCTCTCCCC+CGG | 0.517737 | 3:+48492607 | None:intergenic |
| AAAATATTTACTCATGGGTG+GGG | 0.521587 | 3:-48492678 | None:intergenic |
| ACACAAATGTATAACTCCCC+TGG | 0.530663 | 3:-48492560 | MsG0380013948.01.T01:CDS |
| CAGGAGTCCATTGATGAGAA+GGG | 0.542754 | 3:+48492480 | None:intergenic |
| GAGCTTCAAGAAATAGTGAA+TGG | 0.546870 | 3:-48492593 | MsG0380013948.01.T01:CDS |
| TCAGGAGTCCATTGATGAGA+AGG | 0.551074 | 3:+48492479 | None:intergenic |
| CCATTGATGAGAAGGGGCTA+GGG | 0.552174 | 3:+48492487 | None:intergenic |
| ATAAAATATTTACTCATGGG+TGG | 0.560266 | 3:-48492680 | None:intergenic |
| TAAGCGCAATTGAAATTCCA+GGG | 0.565360 | 3:+48492543 | None:intergenic |
| TGGCTATATGGTTCGTCCGG+GGG | 0.574450 | 3:-48492623 | MsG0380013948.01.T01:CDS |
| TTGGCTATATGGTTCGTCCG+GGG | 0.582473 | 3:-48492624 | MsG0380013948.01.T01:CDS |
| TGGACTCCTGACGTATATGA+AGG | 0.588163 | 3:-48492467 | MsG0380013948.01.T01:CDS |
| CATCTCAGAAATAGATAGAG+AGG | 0.593972 | 3:+48492418 | None:intergenic |
| GCGCTTATATTCATCACTGT+AGG | 0.594447 | 3:-48492527 | MsG0380013948.01.T01:CDS |
| AGTTATGTCTTGGTCCGAGT+GGG | 0.616525 | 3:+48492348 | None:intergenic |
| AGGAGTCCATTGATGAGAAG+GGG | 0.632944 | 3:+48492481 | None:intergenic |
| TCAGAAATAGATAGAGAGGT+CGG | 0.633834 | 3:+48492422 | None:intergenic |
| AAGTTATGTCTTGGTCCGAG+TGG | 0.648471 | 3:+48492347 | None:intergenic |
| AGAGACATGCTATTCCCACT+CGG | 0.691777 | 3:-48492362 | MsG0380013948.01.T01:CDS |
| CTGACGTATATGAAGGAGTG+CGG | 0.702764 | 3:-48492460 | MsG0380013948.01.T01:CDS |
| AAGCGCAATTGAAATTCCAG+GGG | 0.720091 | 3:+48492544 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAACAAGTAAAGTTATGTCT+TGG | + | Chr3:48492667-48492686 | None:intergenic | 25.0% |
| !!! | TTGGATTTTTCAAAACTCTA+TGG | - | Chr3:48492608-48492627 | MsG0380013948.01.T01:CDS | 25.0% |
| ATATTCATCACTGTAGGAAT+TGG | - | Chr3:48492481-48492500 | MsG0380013948.01.T01:CDS | 30.0% | |
| TATTCATCACTGTAGGAATT+GGG | - | Chr3:48492482-48492501 | MsG0380013948.01.T01:CDS | 30.0% | |
| !! | CTATCTATTTCTGAGATGTT+TGG | - | Chr3:48492589-48492608 | MsG0380013948.01.T01:CDS | 30.0% |
| ATAAGCGCAATTGAAATTCC+AGG | + | Chr3:48492463-48492482 | None:intergenic | 35.0% | |
| CATCTCAGAAATAGATAGAG+AGG | + | Chr3:48492587-48492606 | None:intergenic | 35.0% | |
| GAGCTTCAAGAAATAGTGAA+TGG | - | Chr3:48492409-48492428 | MsG0380013948.01.T01:CDS | 35.0% | |
| TAAGCGCAATTGAAATTCCA+GGG | + | Chr3:48492462-48492481 | None:intergenic | 35.0% | |
| TCAGAAATAGATAGAGAGGT+CGG | + | Chr3:48492583-48492602 | None:intergenic | 35.0% | |
| AAGCGCAATTGAAATTCCAG+GGG | + | Chr3:48492461-48492480 | None:intergenic | 40.0% | |
| ACACAAATGTATAACTCCCC+TGG | - | Chr3:48492442-48492461 | MsG0380013948.01.T01:CDS | 40.0% | |
| CATGGTTTCTCTTGGCTATA+TGG | - | Chr3:48492367-48492386 | MsG0380013948.01.T01:CDS | 40.0% | |
| GCGCTTATATTCATCACTGT+AGG | - | Chr3:48492475-48492494 | MsG0380013948.01.T01:CDS | 40.0% | |
| !! | AGCTCTTCTATTCTGGTTCA+TGG | - | Chr3:48492349-48492368 | MsG0380013948.01.T01:CDS | 40.0% |
| !! | TTCTGGTTCATGGTTTCTCT+TGG | - | Chr3:48492359-48492378 | MsG0380013948.01.T01:CDS | 40.0% |
| AAGTTATGTCTTGGTCCGAG+TGG | + | Chr3:48492658-48492677 | None:intergenic | 45.0% | |
| AGAGACATGCTATTCCCACT+CGG | - | Chr3:48492640-48492659 | MsG0380013948.01.T01:CDS | 45.0% | |
| AGGAGTCCATTGATGAGAAG+GGG | + | Chr3:48492524-48492543 | None:intergenic | 45.0% | |
| AGTTATGTCTTGGTCCGAGT+GGG | + | Chr3:48492657-48492676 | None:intergenic | 45.0% | |
| CAGGAGTCCATTGATGAGAA+GGG | + | Chr3:48492525-48492544 | None:intergenic | 45.0% | |
| CTGACGTATATGAAGGAGTG+CGG | - | Chr3:48492542-48492561 | MsG0380013948.01.T01:CDS | 45.0% | |
| TCAGGAGTCCATTGATGAGA+AGG | + | Chr3:48492526-48492545 | None:intergenic | 45.0% | |
| TCTTGGCTATATGGTTCGTC+CGG | - | Chr3:48492376-48492395 | MsG0380013948.01.T01:CDS | 45.0% | |
| TGGACTCCTGACGTATATGA+AGG | - | Chr3:48492535-48492554 | MsG0380013948.01.T01:CDS | 45.0% | |
| CCATTGATGAGAAGGGGCTA+GGG | + | Chr3:48492518-48492537 | None:intergenic | 50.0% | |
| CGCACTCCTTCATATACGTC+AGG | + | Chr3:48492544-48492563 | None:intergenic | 50.0% | |
| CTTGGCTATATGGTTCGTCC+GGG | - | Chr3:48492377-48492396 | MsG0380013948.01.T01:CDS | 50.0% | |
| TCCATTGATGAGAAGGGGCT+AGG | + | Chr3:48492519-48492538 | None:intergenic | 50.0% | |
| TGGGGCAAGCTCTTCTATTC+TGG | - | Chr3:48492342-48492361 | MsG0380013948.01.T01:CDS | 50.0% | |
| TTGAAGCTCTATCTCTCCCC+CGG | + | Chr3:48492398-48492417 | None:intergenic | 50.0% | |
| TTGGCTATATGGTTCGTCCG+GGG | - | Chr3:48492378-48492397 | MsG0380013948.01.T01:CDS | 50.0% | |
| CCCTAGCCCCTTCTCATCAA+TGG | - | Chr3:48492515-48492534 | MsG0380013948.01.T01:CDS | 55.0% | |
| TGGCTATATGGTTCGTCCGG+GGG | - | Chr3:48492379-48492398 | MsG0380013948.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 48492337 | 48492687 | 48492337 | ID=MsG0380013948.01;Name=MsG0380013948.01 |
| Chr3 | mRNA | 48492337 | 48492687 | 48492337 | ID=MsG0380013948.01.T01;Parent=MsG0380013948.01;Name=MsG0380013948.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|116 |
| Chr3 | exon | 48492337 | 48492687 | 48492337 | ID=MsG0380013948.01.T01:exon:102;Parent=MsG0380013948.01.T01 |
| Chr3 | CDS | 48492337 | 48492687 | 48492337 | ID=MsG0380013948.01.T01:cds;Parent=MsG0380013948.01.T01 |
| Gene Sequence |
| Protein sequence |
