AlfalfaGEDB | MsG0680032476.01

M. sativa cultivar ZhongmuNo.1 / MsG0680032476.01

KEGG: -
GO: GO:0030001 GO:0046872
PFAM: PF00403

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032476.01.T01	XP_013448450.1	76.667	90	21	0	1	90	20	109	1.82E-43	147

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032476.01.T01	A0A072TYH4	76.667	90	21	0	1	90	20	109	8.71e-44	147

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000824.01	MsG0680032476.01	0.802776	4.777107e-49	2.360434e-46
MsG0180002075.01	MsG0680032476.01	0.803675	3.112457e-49	1.573593e-46
MsG0180003371.01	MsG0680032476.01	0.809838	1.552567e-50	9.212963e-48
MsG0180005233.01	MsG0680032476.01	0.807451	5.023082e-50	2.798800e-47
MsG0280010551.01	MsG0680032476.01	0.804503	2.094274e-49	1.081562e-46
MsG0280011258.01	MsG0680032476.01	0.804455	2.142453e-49	1.105123e-46
MsG0380013549.01	MsG0680032476.01	0.821623	3.658210e-53	2.991588e-50
MsG0680032476.01	MsG0680032896.01	0.801927	7.146882e-49	3.455608e-46
MsG0680032476.01	MsG0680034655.01	0.812719	3.680892e-51	2.357768e-48
MsG0680032476.01	MsG0780038443.01	0.801473	8.858442e-49	4.234187e-46
MsG0680032476.01	MsG0880043252.01	0.805007	1.643481e-49	8.599039e-47
MsG0480020167.01	MsG0680032476.01	0.823808	1.134421e-53	9.871393e-51
MsG0480022115.01	MsG0680032476.01	0.803818	2.906795e-49	1.475110e-46
MsG0380016765.01	MsG0680032476.01	0.804234	2.381815e-49	1.221597e-46
MsG0580027830.01	MsG0680032476.01	0.800840	1.193441e-48	5.614084e-46
MsG0280007547.01	MsG0680032476.01	0.817298	3.546597e-52	2.572839e-49
MsG0280007764.01	MsG0680032476.01	0.821769	3.384977e-53	2.779758e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032476.01.T01	MTR_7g451210	76.667	90	21	0	1	90	20	109	2.21e-47	147
MsG0680032476.01.T01	MTR_1173s0010	72.527	91	25	0	1	91	20	110	8.29e-46	142
MsG0680032476.01.T01	MTR_3g434890	72.527	91	25	0	1	91	20	110	2.08e-45	142
MsG0680032476.01.T01	MTR_7g451200	72.527	91	25	0	1	91	20	110	4.39e-45	141
MsG0680032476.01.T01	MTR_3g434960	72.527	91	25	0	1	91	20	110	4.95e-45	140
MsG0680032476.01.T01	MTR_3g435000	74.118	85	22	0	1	85	24	108	4.46e-41	130
MsG0680032476.01.T01	MTR_6g049260	68.132	91	29	0	1	91	20	110	4.48e-41	130
MsG0680032476.01.T01	MTR_3g434920	71.084	83	24	0	9	91	2	84	3.91e-40	127
MsG0680032476.01.T01	MTR_6g049320	54.545	88	38	1	1	86	20	107	2.93e-24	89.0
MsG0680032476.01.T01	MTR_6g049280	53.409	88	39	1	1	86	20	107	6.93e-24	87.8
MsG0680032476.01.T01	MTR_6g049320	54.545	88	38	1	1	86	50	137	7.28e-24	89.0
MsG0680032476.01.T01	MTR_6g049280	53.409	88	39	1	1	86	50	137	1.64e-23	87.8
MsG0680032476.01.T01	MTR_1g099800	64.583	48	17	0	1	48	20	67	4.25e-17	70.9
MsG0680032476.01.T01	MTR_1g099810	66.667	48	16	0	1	48	1	48	7.80e-17	69.7
MsG0680032476.01.T01	MTR_1g099810	66.667	48	16	0	1	48	20	67	1.48e-16	69.7
MsG0680032476.01.T01	MTR_8g098915	62.264	53	20	0	1	53	1	53	1.15e-15	66.6
MsG0680032476.01.T01	MTR_8g098915	62.264	53	20	0	1	53	20	72	2.20e-15	66.2
MsG0680032476.01.T01	MTR_6g051680	56.250	48	21	0	1	48	20	67	5.46e-15	64.7
MsG0680032476.01.T01	MTR_6g051690	56.250	48	21	0	1	48	20	67	1.66e-14	63.9
MsG0680032476.01.T01	MTR_1g099640	42.268	97	44	2	1	85	20	116	3.07e-14	63.5
MsG0680032476.01.T01	MTR_7g079110	58.333	48	20	0	1	48	20	67	1.40e-13	62.8
MsG0680032476.01.T01	MTR_1g099815	45.833	96	36	3	1	81	20	114	3.08e-13	60.8
MsG0680032476.01.T01	MTR_8g028615	60.870	46	18	0	3	48	22	67	4.08e-13	61.2
MsG0680032476.01.T01	MTR_1g102240	45.833	96	36	3	1	81	20	114	7.78e-13	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032476.01.T01	AT1G01490	57.143	49	20	1	1	48	47	95	2.18e-11	57.4
MsG0680032476.01.T01	AT1G01490	57.143	49	20	1	1	48	20	68	2.69e-11	57.0
MsG0680032476.01.T01	AT1G01490	57.143	49	20	1	1	48	20	68	2.69e-11	57.0

Find 17 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCTTGGACACTGCCTTTAC+TGG	0.181442	6:-42370177	None:intergenic
AAGACAGCATCTAGCCTTTC+AGG	0.206805	6:+42369631	MsG0680032476.01.T01:CDS
CGAGAAGAAATTGACATTAA+AGG	0.241272	6:+42370143	MsG0680032476.01.T01:CDS
GAGAAGAAATTGACATTAAA+GGG	0.319300	6:+42370144	MsG0680032476.01.T01:CDS
TAGGTTTCAAAGTATTGAAT+TGG	0.328085	6:-42370285	None:intergenic
CAGTGTCCAAGCTAAGAAAA+TGG	0.399021	6:+42370187	MsG0680032476.01.T01:CDS
TTCTGTTGAATCTCTAAAAG+AGG	0.445440	6:+42370227	MsG0680032476.01.T01:CDS
GGGGTCATCTGATGGTAATA+CGG	0.511171	6:-42370309	None:intergenic
AATCTCGAAAATATTGTGGT+GGG	0.543098	6:-42370329	None:intergenic
TAATCTCGAAAATATTGTGG+TGG	0.564645	6:-42370330	None:intergenic
CATCTGATGGTAATACGGAT+AGG	0.572481	6:-42370304	None:intergenic
AAATTCAATATGTACCTGAA+AGG	0.592630	6:-42369645	None:intergenic
ATCTCGAAAATATTGTGGTG+GGG	0.596272	6:-42370328	None:intergenic
TAGTAATCTCGAAAATATTG+TGG	0.619288	6:-42370333	None:intergenic
AGAAGAAATTGACATTAAAG+GGG	0.621172	6:+42370145	MsG0680032476.01.T01:CDS
ATTGTGGTGGGGTCATCTGA+TGG	0.647064	6:-42370317	None:intergenic
GAAGAAATTGACATTAAAGG+GGG	0.651809	6:+42370146	MsG0680032476.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTTTATTTTTATTTTGC+AGG	+	Chr6:42370093-42370112	MsG0680032476.01.T01:intron	10.0%
!!!	TTTTTTATTTTTATTTTGCA+GGG	+	Chr6:42370094-42370113	MsG0680032476.01.T01:intron	10.0%
!!!	TTTTTATTTTTATTTTGCAG+GGG	+	Chr6:42370095-42370114	MsG0680032476.01.T01:intron	15.0%
!!	AAAAATAAAATCGATCATGA+TGG	-	Chr6:42369817-42369836	None:intergenic	20.0%
!!	CACAAAAGTCAAATTAAATT+AGG	-	Chr6:42370037-42370056	None:intergenic	20.0%
!	AAATTCAATATGTACCTGAA+AGG	-	Chr6:42369648-42369667	None:intergenic	25.0%
!	AAGTTGTGTTCAAAAGTTTA+GGG	+	Chr6:42369716-42369735	MsG0680032476.01.T01:intron	25.0%
!	AATGAATCTTTCGTGTTATA+TGG	-	Chr6:42370076-42370095	None:intergenic	25.0%
!	AGAAGAAATTGACATTAAAG+GGG	+	Chr6:42370145-42370164	MsG0680032476.01.T01:CDS	25.0%
!	GAGAAGAAATTGACATTAAA+GGG	+	Chr6:42370144-42370163	MsG0680032476.01.T01:CDS	25.0%
!	TAAGTTGTGTTCAAAAGTTT+AGG	+	Chr6:42369715-42369734	MsG0680032476.01.T01:intron	25.0%
!	TAGTAATCTCGAAAATATTG+TGG	-	Chr6:42370336-42370355	None:intergenic	25.0%
!	TGAAGATGATAAAAGATTAC+AGG	-	Chr6:42369785-42369804	None:intergenic	25.0%
!!!	TAGGTTTCAAAGTATTGAAT+TGG	-	Chr6:42370288-42370307	None:intergenic	25.0%
	AATCTCGAAAATATTGTGGT+GGG	-	Chr6:42370332-42370351	None:intergenic	30.0%
	AGTTGTGTTCAAAAGTTTAG+GGG	+	Chr6:42369717-42369736	MsG0680032476.01.T01:intron	30.0%
	CGAGAAGAAATTGACATTAA+AGG	+	Chr6:42370143-42370162	MsG0680032476.01.T01:CDS	30.0%
	GAAGAAATTGACATTAAAGG+GGG	+	Chr6:42370146-42370165	MsG0680032476.01.T01:CDS	30.0%
	TAATCTCGAAAATATTGTGG+TGG	-	Chr6:42370333-42370352	None:intergenic	30.0%
!	TTCTGTTGAATCTCTAAAAG+AGG	+	Chr6:42370227-42370246	MsG0680032476.01.T01:CDS	30.0%
!!!	TAGGTTTTATTCTCAAACGA+CGG	-	Chr6:42370018-42370037	None:intergenic	30.0%
	ATCTCGAAAATATTGTGGTG+GGG	-	Chr6:42370331-42370350	None:intergenic	35.0%
	GTTGTGTTCAAAAGTTTAGG+GGG	+	Chr6:42369718-42369737	MsG0680032476.01.T01:intron	35.0%
	TACACTTGCTCAAAACAGAA+AGG	+	Chr6:42369861-42369880	MsG0680032476.01.T01:intron	35.0%
	TTGTGTTCAAAAGTTTAGGG+GGG	+	Chr6:42369719-42369738	MsG0680032476.01.T01:intron	35.0%
!	TTGACACCATTTTCTTAGCT+TGG	-	Chr6:42370196-42370215	None:intergenic	35.0%
	CAGTGTCCAAGCTAAGAAAA+TGG	+	Chr6:42370187-42370206	MsG0680032476.01.T01:CDS	40.0%
	CATCTGATGGTAATACGGAT+AGG	-	Chr6:42370307-42370326	None:intergenic	40.0%
	GGGGGATATTGATCCAGTAA+AGG	+	Chr6:42370164-42370183	MsG0680032476.01.T01:CDS	45.0%
	GGGGTCATCTGATGGTAATA+CGG	-	Chr6:42370312-42370331	None:intergenic	45.0%
!	AAGACAGCATCTAGCCTTTC+AGG	+	Chr6:42369631-42369650	MsG0680032476.01.T01:CDS	45.0%
!!	TAACTTTATAAAAAAATATA+TGG	+	Chr6:42369928-42369947	MsG0680032476.01.T01:intron	5.0%
!!!	ATATATTTTTTTATAAAGTT+AGG	-	Chr6:42369929-42369948	None:intergenic	5.0%
	AGCTTGGACACTGCCTTTAC+TGG	-	Chr6:42370180-42370199	None:intergenic	50.0%
!	ATTGTGGTGGGGTCATCTGA+TGG	-	Chr6:42370320-42370339	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	42369628	42370365	42369628	ID=MsG0680032476.01;Name=MsG0680032476.01
Chr6	mRNA	42369628	42370365	42369628	ID=MsG0680032476.01.T01;Parent=MsG0680032476.01;Name=MsG0680032476.01.T01;_AED=0.42;_eAED=0.44;_QI=0\|0\|0\|1\|0\|0\|2\|0\|91
Chr6	exon	42370115	42370365	42370115	ID=MsG0680032476.01.T01:exon:16355;Parent=MsG0680032476.01.T01
Chr6	exon	42369628	42369652	42369628	ID=MsG0680032476.01.T01:exon:16354;Parent=MsG0680032476.01.T01
Chr6	CDS	42369628	42369652	42369628	ID=MsG0680032476.01.T01:cds;Parent=MsG0680032476.01.T01
Chr6	CDS	42370115	42370365	42370115	ID=MsG0680032476.01.T01:cds;Parent=MsG0680032476.01.T01

Gene Sequence	>MsG0680032476.01.T01 ATGAAGACAGCATCTAGCCTTTCAGGGGTTGAATCAATATCAATAGACTTGAACGAGAAGAAATTGACATTAAAGGGGGATATTGATCCAGTAAAGGCAGTGTCCAAGCTAAGAAAATGGTGTCAAACTGAAATAGTTTCTGTTGAATCTCTAAAAGAGGAGACGAAAGAGTCAATAAATACAAATGAAATTATTCCAATTCAATACTTTGAAACCTATCCGTATTACCATCAGATGACCCCACCACAATATTTTCGAGATTACTACTATGTTTGA
Protein sequence	>MsG0680032476.01.T01 MKTASSLSGVESISIDLNEKKLTLKGDIDPVKAVSKLRKWCQTEIVSVESLKEETKESINTNEIIPIQYFETYPYYHQMTPPQYFRDYYYV*