Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003371.01.T01 | XP_013468015.1 | 94.828 | 116 | 5 | 1 | 1 | 115 | 1 | 116 | 8.97E-73 | 222 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003371.01.T01 | A0A072VJR4 | 94.828 | 116 | 5 | 1 | 1 | 115 | 1 | 116 | 4.28e-73 | 222 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048856.01 | MsG0180003371.01 | 0.808038 | 3.768970e-50 | 2.132361e-47 |
| MsG0180000178.01 | MsG0180003371.01 | 0.807681 | 4.487800e-50 | 2.515461e-47 |
| MsG0180000824.01 | MsG0180003371.01 | 0.801285 | 9.679314e-49 | 4.604707e-46 |
| MsG0180001892.01 | MsG0180003371.01 | 0.806804 | 6.883732e-50 | 3.771644e-47 |
| MsG0180003094.01 | MsG0180003371.01 | 0.816636 | 4.994343e-52 | 3.558010e-49 |
| MsG0180003361.01 | MsG0180003371.01 | 0.814202 | 1.737197e-51 | 1.158175e-48 |
| MsG0180003371.01 | MsG0180003920.01 | 0.806264 | 8.950918e-50 | 4.836992e-47 |
| MsG0180003371.01 | MsG0180004099.01 | 0.815446 | 9.207870e-52 | 6.349842e-49 |
| MsG0180003371.01 | MsG0180004743.01 | 0.800077 | 1.706846e-48 | 7.875063e-46 |
| MsG0180003371.01 | MsG0180004754.01 | 0.844342 | 8.022063e-59 | 1.291185e-55 |
| MsG0180003371.01 | MsG0180005480.01 | 0.805289 | 1.434153e-49 | 7.558461e-47 |
| MsG0180003371.01 | MsG0180005575.01 | 0.826769 | 2.262037e-54 | 2.141415e-51 |
| MsG0180003371.01 | MsG0280007452.01 | 0.811655 | 6.279741e-51 | 3.909280e-48 |
| MsG0180003371.01 | MsG0280007491.01 | 0.803727 | 3.036528e-49 | 1.537303e-46 |
| MsG0180003371.01 | MsG0280007955.01 | 0.807242 | 5.560980e-50 | 3.081736e-47 |
| MsG0180003371.01 | MsG0280008614.01 | 0.803227 | 3.855025e-49 | 1.926717e-46 |
| MsG0180003371.01 | MsG0280008615.01 | 0.805793 | 1.124464e-49 | 6.003684e-47 |
| MsG0180003371.01 | MsG0280008909.01 | 0.811157 | 8.057745e-51 | 4.950795e-48 |
| MsG0180003371.01 | MsG0280009514.01 | 0.811747 | 5.996991e-51 | 3.742580e-48 |
| MsG0180003371.01 | MsG0280009794.01 | 0.830845 | 2.334173e-55 | 2.488938e-52 |
| MsG0180003371.01 | MsG0280010491.01 | 0.811038 | 8.548762e-51 | 5.236407e-48 |
| MsG0180003371.01 | MsG0280010907.01 | 0.808911 | 2.453592e-50 | 1.420378e-47 |
| MsG0180003371.01 | MsG0280011003.01 | 0.801274 | 9.727331e-49 | 4.626342e-46 |
| MsG0180003371.01 | MsG0280011013.01 | 0.812737 | 3.647331e-51 | 2.337400e-48 |
| MsG0180003371.01 | MsG0280011014.01 | 0.831331 | 1.773434e-55 | 1.918359e-52 |
| MsG0180003371.01 | MsG0280011258.01 | 0.811563 | 6.574931e-51 | 4.083429e-48 |
| MsG0180003371.01 | MsG0280011282.01 | 0.804267 | 2.344334e-49 | 1.203421e-46 |
| MsG0180003371.01 | MsG0280011388.01 | 0.823337 | 1.462307e-53 | 1.255445e-50 |
| MsG0180003371.01 | MsG0380013183.01 | 0.800528 | 1.381706e-48 | 6.448163e-46 |
| MsG0180003371.01 | MsG0380013475.01 | 0.822506 | 2.284612e-53 | 1.915957e-50 |
| MsG0180003371.01 | MsG0380015677.01 | 0.809654 | 1.700550e-50 | 1.004240e-47 |
| MsG0180003371.01 | MsG0380015944.01 | 0.810488 | 1.124112e-50 | 6.785051e-48 |
| MsG0180003371.01 | MsG0380016131.01 | 0.810466 | 1.136435e-50 | 6.855482e-48 |
| MsG0180003371.01 | MsG0380016247.01 | 0.818682 | 1.725610e-52 | 1.300366e-49 |
| MsG0180003371.01 | MsG0380016994.01 | 0.811175 | 7.984762e-51 | 4.908380e-48 |
| MsG0180003371.01 | MsG0380017996.01 | 0.816496 | 5.367151e-52 | 3.808901e-49 |
| MsG0180003371.01 | MsG0480018313.01 | 0.830034 | 3.685959e-55 | 3.838202e-52 |
| MsG0180003371.01 | MsG0480018894.01 | 0.801831 | 7.480188e-49 | 3.607995e-46 |
| MsG0180003371.01 | MsG0480019922.01 | 0.818283 | 2.124712e-52 | 1.583600e-49 |
| MsG0180003371.01 | MsG0480020780.01 | 0.816324 | 5.864877e-52 | 4.143049e-49 |
| MsG0180003371.01 | MsG0480020879.01 | 0.809880 | 1.520149e-50 | 9.030447e-48 |
| MsG0180003371.01 | MsG0480022620.01 | 0.838460 | 2.838232e-57 | 3.804228e-54 |
| MsG0180003371.01 | MsG0480023463.01 | 0.806351 | 8.582535e-50 | 4.647907e-47 |
| MsG0180003371.01 | MsG0580026059.01 | 0.801157 | 1.027765e-48 | 4.873787e-46 |
| MsG0180003371.01 | MsG0580026593.01 | 0.802456 | 5.562311e-49 | 2.725969e-46 |
| MsG0180003371.01 | MsG0580027431.01 | 0.815376 | 9.542285e-52 | 6.567680e-49 |
| MsG0180003371.01 | MsG0580027650.01 | 0.812708 | 3.700556e-51 | 2.369653e-48 |
| MsG0180003371.01 | MsG0580029127.01 | 0.823650 | 1.235745e-53 | 1.070517e-50 |
| MsG0180003371.01 | MsG0580029729.01 | 0.813790 | 2.141879e-51 | 1.412133e-48 |
| MsG0180003371.01 | MsG0680030447.01 | 0.810165 | 1.319880e-50 | 7.899241e-48 |
| MsG0180003371.01 | MsG0680031549.01 | 0.801778 | 7.668146e-49 | 3.693604e-46 |
| MsG0180003371.01 | MsG0680032476.01 | 0.809838 | 1.552567e-50 | 9.212963e-48 |
| MsG0180003371.01 | MsG0680035475.01 | 0.808910 | 2.455575e-50 | 1.421467e-47 |
| MsG0180003371.01 | MsG0780036477.01 | 0.814014 | 1.911618e-51 | 1.268014e-48 |
| MsG0180003371.01 | MsG0780036815.01 | 0.806975 | 6.336649e-50 | 3.487137e-47 |
| MsG0180003371.01 | MsG0780037054.01 | 0.829853 | 4.079109e-55 | 4.226474e-52 |
| MsG0180003371.01 | MsG0780037460.01 | 0.806132 | 9.543933e-50 | 5.139874e-47 |
| MsG0180003371.01 | MsG0780038188.01 | 0.811883 | 5.601265e-51 | 3.508190e-48 |
| MsG0180003371.01 | MsG0780038424.01 | 0.837142 | 6.188808e-57 | 7.966210e-54 |
| MsG0180003371.01 | MsG0780040926.01 | 0.841180 | 5.556714e-58 | 8.098945e-55 |
| MsG0180003371.01 | MsG0780041535.01 | 0.811007 | 8.684278e-51 | 5.315449e-48 |
| MsG0180003371.01 | MsG0880041986.01 | 0.813948 | 1.976075e-51 | 1.308396e-48 |
| MsG0180003371.01 | MsG0880042129.01 | 0.801763 | 7.724517e-49 | 3.719194e-46 |
| MsG0180003371.01 | MsG0880042961.01 | 0.824025 | 1.009271e-53 | 8.834909e-51 |
| MsG0180003371.01 | MsG0880043034.01 | 0.808994 | 2.356288e-50 | 1.367087e-47 |
| MsG0180003371.01 | MsG0880043479.01 | 0.800929 | 1.144258e-48 | 5.394972e-46 |
| MsG0180003371.01 | MsG0880044782.01 | 0.818938 | 1.509439e-52 | 1.145740e-49 |
| MsG0180003371.01 | MsG0880046012.01 | 0.810584 | 1.071850e-50 | 6.486266e-48 |
| MsG0180003371.01 | MsG0880047473.01 | 0.800757 | 1.240926e-48 | 5.824849e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003371.01.T01 | MTR_1g060430 | 94.828 | 116 | 5 | 1 | 1 | 115 | 1 | 116 | 1.09e-76 | 222 |
| MsG0180003371.01.T01 | MTR_7g104250 | 65.385 | 104 | 25 | 3 | 10 | 112 | 1 | 94 | 1.02e-40 | 130 |
| MsG0180003371.01.T01 | MTR_8g043660 | 59.048 | 105 | 37 | 2 | 6 | 110 | 13 | 111 | 9.55e-36 | 119 |
| MsG0180003371.01.T01 | MTR_8g032890 | 56.604 | 106 | 38 | 3 | 6 | 110 | 13 | 111 | 3.24e-33 | 112 |
| MsG0180003371.01.T01 | MTR_1g101680 | 51.961 | 102 | 38 | 1 | 7 | 108 | 2 | 92 | 5.42e-33 | 111 |
| MsG0180003371.01.T01 | MTR_8g032940 | 55.238 | 105 | 41 | 2 | 6 | 110 | 13 | 111 | 3.84e-29 | 102 |
| MsG0180003371.01.T01 | MTR_1g101750 | 87.755 | 49 | 6 | 0 | 60 | 108 | 6 | 54 | 3.69e-28 | 98.2 |
| MsG0180003371.01.T01 | MTR_5g038200 | 50.000 | 48 | 24 | 0 | 53 | 100 | 27 | 74 | 6.70e-14 | 62.0 |
| MsG0180003371.01.T01 | MTR_7g055590 | 72.222 | 36 | 8 | 1 | 75 | 110 | 461 | 494 | 5.25e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003371.01.T01 | AT3G52790 | 56.604 | 106 | 42 | 2 | 6 | 110 | 5 | 107 | 2.47e-36 | 120 |
| MsG0180003371.01.T01 | AT5G62150 | 80.392 | 51 | 10 | 0 | 61 | 111 | 46 | 96 | 1.20e-27 | 97.8 |
| MsG0180003371.01.T01 | AT4G25433 | 75.510 | 49 | 12 | 0 | 61 | 109 | 57 | 105 | 8.94e-25 | 90.9 |
Find 19 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATTCTCTTCAACAATATA+AGG | 0.330783 | 1:-61283548 | None:intergenic |
| ATATTCAAATTAGCCTAGAT+TGG | 0.387158 | 1:-61283712 | None:intergenic |
| ATGGAACTGTGCACTGTTTG+TGG | 0.421993 | 1:+61283340 | MsG0180003371.01.T01:CDS |
| ATTTATGAACTAACAGAGAA+AGG | 0.438055 | 1:-61283623 | None:intergenic |
| TCTGTGTATGTACCCAATCT+AGG | 0.441471 | 1:+61283699 | MsG0180003371.01.T01:three_prime_UTR |
| CAATGGCGGAGAAGATTTCA+TGG | 0.451007 | 1:+61283321 | MsG0180003371.01.T01:CDS |
| ACTGTTTGTGGCATTGATGT+TGG | 0.479292 | 1:+61283352 | MsG0180003371.01.T01:CDS |
| TTTATGAACTAACAGAGAAA+GGG | 0.480365 | 1:-61283622 | None:intergenic |
| AGGCCAGGGAAAACATCATC+AGG | 0.484403 | 1:-61283584 | None:intergenic |
| TATTCAAATTAGCCTAGATT+GGG | 0.494140 | 1:-61283711 | None:intergenic |
| TTAATCTTGATAACAAGGCC+AGG | 0.525582 | 1:-61283599 | None:intergenic |
| TCATCAGGATCATGAATATG+TGG | 0.554352 | 1:-61283569 | None:intergenic |
| TGTGATGAGATATACGTTGT+TGG | 0.555389 | 1:+61283482 | MsG0180003371.01.T01:CDS |
| GAGATATACGTTGTTGGCGA+AGG | 0.561886 | 1:+61283488 | MsG0180003371.01.T01:CDS |
| AAGGGTTAATCTTGATAACA+AGG | 0.616888 | 1:-61283604 | None:intergenic |
| ACACAAATCAGAATGGCTTG+TGG | 0.618503 | 1:+61283284 | None:intergenic |
| TAATCTTGATAACAAGGCCA+GGG | 0.622108 | 1:-61283598 | None:intergenic |
| TGGATGTTCATCAACAGCAA+TGG | 0.627186 | 1:+61283304 | MsG0180003371.01.T01:CDS |
| ATGTTCATCAACAGCAATGG+CGG | 0.639515 | 1:+61283307 | MsG0180003371.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTATGTGTTTTTAAAATTGA+AGG | + | Chr1:61283673-61283692 | MsG0180003371.01.T01:three_prime_UTR | 20.0% |
| ! | ATTTATGAACTAACAGAGAA+AGG | - | Chr1:61283626-61283645 | None:intergenic | 25.0% |
| ! | CAAACAATAAGTGAAAAATG+TGG | + | Chr1:61283521-61283540 | MsG0180003371.01.T01:CDS | 25.0% |
| ! | TTTATGAACTAACAGAGAAA+GGG | - | Chr1:61283625-61283644 | None:intergenic | 25.0% |
| !!! | TTTCAGCTTTTAATTTGATC+AGG | + | Chr1:61283649-61283668 | MsG0180003371.01.T01:three_prime_UTR | 25.0% |
| GGATTCTCTTCAACAATATA+AGG | - | Chr1:61283551-61283570 | None:intergenic | 30.0% | |
| ! | AAGGGTTAATCTTGATAACA+AGG | - | Chr1:61283607-61283626 | None:intergenic | 30.0% |
| TCATCAGGATCATGAATATG+TGG | - | Chr1:61283572-61283591 | None:intergenic | 35.0% | |
| TGTGATGAGATATACGTTGT+TGG | + | Chr1:61283482-61283501 | MsG0180003371.01.T01:CDS | 35.0% | |
| ! | TAATCTTGATAACAAGGCCA+GGG | - | Chr1:61283601-61283620 | None:intergenic | 35.0% |
| ! | TTAATCTTGATAACAAGGCC+AGG | - | Chr1:61283602-61283621 | None:intergenic | 35.0% |
| ATGTTCATCAACAGCAATGG+CGG | + | Chr1:61283307-61283326 | MsG0180003371.01.T01:CDS | 40.0% | |
| TCTGTGTATGTACCCAATCT+AGG | + | Chr1:61283699-61283718 | MsG0180003371.01.T01:three_prime_UTR | 40.0% | |
| TGGATGTTCATCAACAGCAA+TGG | + | Chr1:61283304-61283323 | MsG0180003371.01.T01:CDS | 40.0% | |
| !! | ACTGTTTGTGGCATTGATGT+TGG | + | Chr1:61283352-61283371 | MsG0180003371.01.T01:CDS | 40.0% |
| ATGGAACTGTGCACTGTTTG+TGG | + | Chr1:61283340-61283359 | MsG0180003371.01.T01:CDS | 45.0% | |
| GAGATATACGTTGTTGGCGA+AGG | + | Chr1:61283488-61283507 | MsG0180003371.01.T01:CDS | 45.0% | |
| ! | CAATGGCGGAGAAGATTTCA+TGG | + | Chr1:61283321-61283340 | MsG0180003371.01.T01:CDS | 45.0% |
| !! | GATCCTGATGATGTTTTCCC+TGG | + | Chr1:61283581-61283600 | MsG0180003371.01.T01:CDS | 45.0% |
| AGGCCAGGGAAAACATCATC+AGG | - | Chr1:61283587-61283606 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 61283296 | 61283721 | 61283296 | ID=MsG0180003371.01;Name=MsG0180003371.01 |
| Chr1 | mRNA | 61283296 | 61283721 | 61283296 | ID=MsG0180003371.01.T01;Parent=MsG0180003371.01;Name=MsG0180003371.01.T01;_AED=0.03;_eAED=0.03;_QI=0|-1|0|1|-1|1|1|78|115 |
| Chr1 | exon | 61283296 | 61283721 | 61283296 | ID=MsG0180003371.01.T01:exon:4225;Parent=MsG0180003371.01.T01 |
| Chr1 | CDS | 61283296 | 61283643 | 61283296 | ID=MsG0180003371.01.T01:cds;Parent=MsG0180003371.01.T01 |
| Chr1 | three_prime_UTR | 61283644 | 61283721 | 61283644 | ID=MsG0180003371.01.T01:three_prime_utr;Parent=MsG0180003371.01.T01 |
| Gene Sequence |
| Protein sequence |
